RchiOBHm_Chr3g0480711

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0016410
Physical Location & Seq
Reverse (-)
26533561 .. 26537816
4256 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44572

Sequence Viewer

Length: 528 bp
ATGAGAGATTATGCAGTGACTAAATGCCAGTTATTTCGTCACATGAGTGATGCCAAGCTTGGGCTTCTTTGTTTTGGGAACCAGTACTTAAATGAAGCAGTTATGGGAAGCTTGAATGAAATTAATCTTGCTTGGATAGGAGCCACTCCAGGCGTAAGAATTGATATGGAAGCAAAAATTGCAAGCTTTGAAGTGCCTGCCTTGGGAATCAATTCTCAACTACAATTGGGCGCAATGACCATGATTGGAACACAGAACTGTCACAATGCTGCAGTTGCTGCTTTATCTGTTGTTGGACTGGATGTAGGACTTGATGTTGAAGCCATTGGTCCATGTATAGAAAAGTTAAGGCCACCACCTCATCGTATACAAATTGTATGCAAGGATATGCACGGGGTAACTTGGGTTGATGACAGCAAGGCAACAAGTGTAGAAGCTACGTACACAGGACTAATGGGTCTTAAAGAAGCAAAAGTCTGTGATTCTACTTGGTGGCCTTGTGAAGGTTGTGGACGGACATGTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2-Hacid_dh_C Mur_ligase_C Mur_ligase_M

Protein Analysis

175

Amino Acids

18.89

Weight (kDa)

5.51

Isoelectric Point (pI)

22.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016410)

Species Orthologous Gene IDs
malus_domestica MD12G1063100.v1.1
prunus_persica Prupe.7G070400_v2.0.a1
pyrus_communis pycom12g05560
rosa_chinensis RchiOBHm_Chr3g0480711 RchiOBHm_Chr3g0480721
rosa_multiflora Rmu_sc0001135.1_g000007 Rmu_sc0003902.1_g000026
rosa_roxburghii Rroxscaffold_6G00401140
rosa_rugosa Rorug03G0185000 Rorug03G0185100
rosa_samantha Rh3AG237200 Rh3BG270300 Rh3CG266500 Rh3DG262700
rosa_wichuraiana Rw3G021240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 456
AccI GTMKAC 1 cut(s) 367
AfaI GTAC 2 cut(s) 86, 443
AfiI CCNNNNNNNGG 3 cut(s) 60, 203, 503
AflIII ACRYGT 1 cut(s) 518
AgsI TTSAA 3 cut(s) 115, 191, 320
AjnI CCWGG 1 cut(s) 148
AleI CACNNNNGTG 1 cut(s) 45
AluBI AGCT 4 cut(s) 58, 111, 186, 437
AluI AGCT 4 cut(s) 58, 111, 186, 437
AlwNI CAGNNNCTG 1 cut(s) 278
AoxI GGCC 2 cut(s) 350, 494
ApeKI GCWGC 2 cut(s) 269, 278
AseI ATTAAT 1 cut(s) 123
Asp700I GAANNNNTTC 1 cut(s) 211
AspLEI GCGC 1 cut(s) 233
AspS9I GGNCC 1 cut(s) 329
AvaII GGWCC 1 cut(s) 329
BbvI GCAGC 2 cut(s) 256, 265
BciT130I CCWGG 1 cut(s) 150
BfmI CTRYAG 1 cut(s) 270
BisI GCNGC 2 cut(s) 270, 279
BlsI GCNGC 2 cut(s) 271, 280
BmcAI AGTACT 1 cut(s) 86
Bme1390I CCNGG 1 cut(s) 150
Bme18I GGWCC 1 cut(s) 329
BmgT120I GGNCC 1 cut(s) 329
BmiI GGNNCC 2 cut(s) 80, 142
BmrFI CCNGG 1 cut(s) 150
BmsI GCATC 1 cut(s) 40
BoxI GACNNNNGTC 1 cut(s) 521
BpmI CTGGAG 1 cut(s) 132
BsaAI YACGTR 1 cut(s) 441
BsaJI CCNNGG 1 cut(s) 201
Bsc4I CCNNNNNNNGG 3 cut(s) 60, 203, 503
Bse1I ACTGG 3 cut(s) 28, 82, 303
Bse3DI GCAATG 1 cut(s) 240
BseBI CCWGG 1 cut(s) 150
BseDI CCNNGG 1 cut(s) 201
BseGI GGATG 1 cut(s) 307
BseLI CCNNNNNNNGG 3 cut(s) 60, 203, 503
BseMI GCAATG 1 cut(s) 240
BseNI ACTGG 3 cut(s) 28, 82, 303
BseXI GCAGC 2 cut(s) 256, 265
BshFI GGCC 2 cut(s) 352, 496
BslI CCNNNNNNNGG 3 cut(s) 60, 203, 503
BsnI GGCC 2 cut(s) 352, 496
BspANI GGCC 2 cut(s) 352, 496
BspLI GGNNCC 2 cut(s) 80, 142
BspMAI CTGCAG 1 cut(s) 274
BsrDI GCAATG 1 cut(s) 240
BsrI ACTGG 3 cut(s) 28, 82, 303
BssECI CCNNGG 1 cut(s) 201
BssNAI GTATAC 1 cut(s) 368
BssT1I CCWWGG 1 cut(s) 201
Bst1107I GTATAC 1 cut(s) 368
Bst2UI CCWGG 1 cut(s) 150
Bst4CI ACNGT 1 cut(s) 260
BstAPI GCANNNNNTGC 2 cut(s) 179, 278
BstBAI YACGTR 1 cut(s) 441
BstC8I GCNNGC 2 cut(s) 184, 198
BstENI CCTNNNNNAGG 1 cut(s) 501
BstF5I GGATG 1 cut(s) 307
BstHHI GCGC 1 cut(s) 233
BstMWI GCNNNNNNNGC 3 cut(s) 179, 275, 278
BstNI CCWGG 1 cut(s) 150
BstNSI RCATGY 1 cut(s) 522
BstPAI GACNNNNGTC 1 cut(s) 521
BstSCI CCNGG 1 cut(s) 148
BstSFI CTRYAG 1 cut(s) 270
BstSNI TACGTA 1 cut(s) 441
BstV1I GCAGC 2 cut(s) 256, 265
BstZ17I GTATAC 1 cut(s) 368
BsuRI GGCC 2 cut(s) 352, 496
BtsCI GGATG 1 cut(s) 307
BtsI GCAGTG 1 cut(s) 21
BtsIMutI CAGTG 1 cut(s) 21
Cac8I GCNNGC 2 cut(s) 184, 198
CaiI CAGNNNCTG 1 cut(s) 278
CfoI GCGC 1 cut(s) 233
Cfr13I GGNCC 1 cut(s) 329
Csp6I GTAC 2 cut(s) 85, 442
CviAII CATG 4 cut(s) 43, 241, 333, 519
CviJI RGCY 9 cut(s) 58, 64, 111, 143, 186, 323, 352, 437, 496
CviKI_1 RGCY 9 cut(s) 58, 64, 111, 143, 186, 323, 352, 437, 496
CviQI GTAC 2 cut(s) 85, 442
DrdI GACNNNNNNGTC 1 cut(s) 456
DseDI GACNNNNNNGTC 1 cut(s) 456
Eco105I TACGTA 1 cut(s) 441
Eco130I CCWWGG 1 cut(s) 201
Eco47I GGWCC 1 cut(s) 329
EcoNI CCTNNNNNAGG 1 cut(s) 501
EcoRII CCWGG 1 cut(s) 148
EcoT14I CCWWGG 1 cut(s) 201
ErhI CCWWGG 1 cut(s) 201
FaeI CATG 4 cut(s) 46, 244, 336, 522
FatI CATG 4 cut(s) 42, 240, 332, 518
FblI GTMKAC 1 cut(s) 367
Fnu4HI GCNGC 2 cut(s) 270, 279
FokI GGATG 1 cut(s) 314
Fsp4HI GCNGC 2 cut(s) 270, 279
GlaI GCGC 1 cut(s) 232
GluI GCNGC 2 cut(s) 270, 279
GsuI CTGGAG 1 cut(s) 132
HaeIII GGCC 2 cut(s) 352, 496
HhaI GCGC 1 cut(s) 233
Hin1II CATG 4 cut(s) 46, 244, 336, 522
Hin6I GCGC 1 cut(s) 231
HinP1I GCGC 1 cut(s) 231
HindIII AAGCTT 3 cut(s) 56, 109, 184
HinfI GANTC 2 cut(s) 207, 482
Hpy166II GTNNAC 3 cut(s) 368, 444, 512
Hpy8I GTNNAC 3 cut(s) 368, 444, 512
HpyAV CCTTC 1 cut(s) 497
HpyCH4III ACNGT 1 cut(s) 260
HpyCH4IV ACGT 1 cut(s) 440
HpyCH4V TGCA 5 cut(s) 14, 182, 272, 381, 391
HpyF10VI GCNNNNNNNGC 3 cut(s) 179, 275, 278
HpySE526I ACGT 1 cut(s) 440
Hsp92II CATG 4 cut(s) 46, 244, 336, 522
HspAI GCGC 1 cut(s) 231
LmnI GCTCC 1 cut(s) 140
LpnPI CCDG 7 cut(s) 41, 95, 135, 162, 210, 284, 432
Lsp1109I GCAGC 2 cut(s) 256, 265
LweI GCATC 1 cut(s) 40
MaeII ACGT 1 cut(s) 440
MaeIII GTNAC 4 cut(s) 16, 38, 260, 397
MfeI CAATTG 1 cut(s) 224
MluCI AATT 6 cut(s) 120, 159, 177, 211, 224, 372
MmeI TCCRAC 1 cut(s) 274
MnlI CCTC 1 cut(s) 369
MroXI GAANNNNTTC 1 cut(s) 211
MseI TTAA 4 cut(s) 89, 123, 347, 462
MslI CAYNNNNRTG 1 cut(s) 45
MspR9I CCNGG 1 cut(s) 150
MunI CAATTG 1 cut(s) 224
MvaI CCWGG 1 cut(s) 150
MwoI GCNNNNNNNGC 3 cut(s) 179, 275, 278
NlaIII CATG 4 cut(s) 46, 244, 336, 522
NlaIV GGNNCC 2 cut(s) 80, 142
NmuCI GTSAC 3 cut(s) 16, 38, 260
NspI RCATGY 1 cut(s) 522
OliI CACNNNNGTG 1 cut(s) 45
PciI ACATGT 1 cut(s) 518
PdmI GAANNNNTTC 1 cut(s) 211
PfeI GAWTC 2 cut(s) 207, 482
PkrI GCNGC 2 cut(s) 271, 280
Ppu21I YACGTR 1 cut(s) 441
PscI ACATGT 1 cut(s) 518
PshAI GACNNNNGTC 1 cut(s) 521
PshBI ATTAAT 1 cut(s) 123
Psp6I CCWGG 1 cut(s) 148
PspGI CCWGG 1 cut(s) 148
PspN4I GGNNCC 2 cut(s) 80, 142
PspPI GGNCC 1 cut(s) 329
PstI CTGCAG 1 cut(s) 274
PstNI CAGNNNCTG 1 cut(s) 278
RsaI GTAC 2 cut(s) 86, 443
RsaNI GTAC 2 cut(s) 85, 442
RseI CAYNNNNRTG 1 cut(s) 45
SaqAI TTAA 4 cut(s) 89, 123, 347, 462
SatI GCNGC 2 cut(s) 270, 279
Sau96I GGNCC 1 cut(s) 329
ScaI AGTACT 1 cut(s) 86
ScrFI CCNGG 1 cut(s) 150
SetI ASST 7 cut(s) 60, 113, 188, 361, 439, 443, 508
SfaNI GCATC 1 cut(s) 40
SfcI CTRYAG 1 cut(s) 270
SinI GGWCC 1 cut(s) 329
SmiMI CAYNNNNRTG 1 cut(s) 45
SnaBI TACGTA 1 cut(s) 441
Sse9I AATT 6 cut(s) 120, 159, 177, 211, 224, 372
StyD4I CCNGG 1 cut(s) 148
StyI CCWWGG 1 cut(s) 201
TaaI ACNGT 1 cut(s) 260
TaiI ACGT 1 cut(s) 443
TasI AATT 6 cut(s) 120, 159, 177, 211, 224, 372
TatI WGTACW 1 cut(s) 84
TfiI GAWTC 2 cut(s) 207, 482
Tru1I TTAA 4 cut(s) 89, 123, 347, 462
Tru9I TTAA 4 cut(s) 89, 123, 347, 462
TscAI CASTG 1 cut(s) 21
TseFI GTSAC 3 cut(s) 16, 38, 260
TseI GCWGC 2 cut(s) 269, 278
Tsp45I GTSAC 3 cut(s) 16, 38, 260
TspDTI ATGAA 2 cut(s) 108, 132
TspRI CASTG 1 cut(s) 21
VpaK11BI GGWCC 1 cut(s) 329
VspI ATTAAT 1 cut(s) 123
XagI CCTNNNNNAGG 1 cut(s) 501
XceI RCATGY 1 cut(s) 522
XmiI GTMKAC 1 cut(s) 367
XmnI GAANNNNTTC 1 cut(s) 211
ZrmI AGTACT 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.