MD12G1075000.v1.1

RRP12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0011076
Physical Location & Seq
Forward (+)
9087498 .. 9099061
11564 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1075000.v1.1.491

Sequence Viewer

Length: 3498 bp
ATGGACGAAGACCACCACCACGACCTAGACGCAGTCGAGCCATTCAACGATGTCGCAGACATTTGCCAACAGCTCATGGACCGCTACAAAAAGTCCTCGGCTCCCCAGCACCGCCACCTCCTCGCCACCGCGGCTGCTATGCGCGCCATCCTCGCCGCCGAGTCCCTCCCCCTCACTCCACCTGCCTATTTCGCTGCCACCATCTCCTCCATCGACGACATGTCTTCATCCGCGTCCCAGACCCTAGAACCCACTGCCACAGCGGCGCTGTTGTCGTTCTTGGCCATGGTGCTCCCGTTGGTGCCTCCGGGGAGCGTCTCGTCGGCCAAGGCGGGCGAGGCTGTGTCGATGCTGATGGGACTTCTGAAGAGAGAGGAGGAGCTATCCATGCCCGCTGTGAGGGCTTTGGTAAAGTGCTTGGGGGTTTTGGTAGGGTTTTGTGATTTGGAGGATTGGGGATCTATCAAATTGGGATTGGAGACGTTACTCAAGTTTTCCGTCGATCGGCGGCCCAAGGTCCGGAAATGTTCTCAAGACTGTCTCGAGAACGTTTTTAAGTCGCTCCAGTCCCGCACTGTTATCAAGGAGGTTAGCAAGTTGATACTGTCTAAGCTCAAAGGATACATGCCTTTGGCGGTTGAATTAAGTGCTTCACAGACATCAGATGGACCTAAAAATCTAGAGGTCCTTCACATGCTAAATGTGGTGAAACTTACCATTCCCTTTCTGTCAAAGAAAGTCGGTTCAAAGCTTCTCTCAGAAATAAATGAGCTTGTTGTTTCACGGTTCTCAACACTCACAAGGCATGTACTCCAAATTATTGAAACGTTGTTTGAGAGCTCAAGAGCTAATGCCATTGTTTCTGAAACAGAGGCGACTATAGCTTCTCTTGCTTCATACGTGTCTTTGGGAGATGAGGTTCCCTCGGACACTGTCATGTTGGCAGCAACCTTGTTAAAATCTTCTTTGTTTATACTTCATGCTGGAGAATCGACATCGTGGATTAATAGTCTTCCTCTAGTTTGTAGTTCTGTGGCAGGTCTTCTGACTTCTGAGGCTAGCACTGCATCACACGCTTCAGATATTCTTAAAGAGCTGATAAGCCAATTTGTGGATCAAAAAAGTCTGCTGGTGGGTGAAAGCCAACCTTCAGATGATGAAGCACAAGAGACTATGAAAGCAAGTGCACTAATATCAATCTGTACTGCTTTTGAGGATGCCCTTAGAACTTGTAAAGGAGTGCCAAATGAGCATCTTTTGGGGGTTATATCGGCATTGTTCCTCAAGCTCGGAGGAATCTCCTATGTCTATATGAGAAATATTCTTCTCAACCTTGCCAACTCAATGACCCTTGCTAGTGGGGACAAATCTAACACCGATCATCTTCAGAAGTGTATTGGAACTGCTGTAATTGCAATTGGACCTGAGAGGATACTTGAACTTGTTCCTATATCTCTCAATGCTAGTGACTTCACCTGTTCAAACATTTGGTTGGTACCTATTTTGAAAGATTATGTTGTTGGAGCATCACTTGGTTACTATATGGAGCATATTATGCCTCTTGCAAATTCCTTTTGTCGGGCCAGTGGTAAAGTTAAAAAGTCAATAACCAGTCAAGATCTACAGGCTCGTGCACGTGATCTGTTGGGATTGCTGCCTGCTTTCTGTAATCTTCCAACTGATATATGCCAAAAATTTCGACCGTTGGCTGAGATTTTAGTAACATTTCTTAAGGACTCCTTAATGCATGAAAATATTGCAGTTGCCTTGCAGGTTCTTGTGAATCAGAACAAAAGTGTACTTGACCAAAAAGATGGTGCTGGTGACACGAAGTCCTATGCAGTAAACAAATCTTTGTTAGAGTTCGGCAACTTACCCACTTATTCAAAGAAAACTGCTACCAGAAACATTAAGGCTTTGACATCATGTTCTTCTGAGCTGCTTATTGCTCTAACAGACTTATTTCTTTATTCACCCCCTGAGAAGCGTTTATATCTAAAGGATGCCATAGGATGCTTGGCTTCAGTAACTGATTCTTCCAATGCCAAGAAGATTTTTATATCATTGCTTGAGAAGTTCCAGTTCAAAGATAGTAGGCATGAGCTTGGAAAGTTGGAGAGTCAGACTGATGCATCAGCTGGTGAGGAAGAACACAACCTAAGTACTAGAGAGAAAGATGCTCTGCGATGCGTGATGATGGAATTAGCATCTTCTTTTGTTAAAGGAGCCAATGAGGATCTTATTGATCTAATATATACGTTTGCTAAAGATACTTTACTGAACGATGATGAGATCGCCATTTATGAAGCATATCATACTTTGAGCAGAATTCTAGAGGAGCATGATTGGTTCTGTTCTTCTCGGTTTGCTGAATTGATTGATCTGCTACTTGGTTTGAGATCTCCTGTTGATATCATATCTCTAAGGAGTCGCTTTGCTAGTTTCCAGACTTTAATGATTCATACTTTGAAGATAGACTCAGAGGTAGAAAATTCAAAGTCGTTTCTTATTCTCAATGAGATCATACTCAAGTTAAAAGATGCCAAGGATGAAGCAGTCAGGGAGGCAGCTTACGATGTACTCCTCAAAATAAGTGTTAGCTTGAGAGATCCATCGTGTGTCAGTTCAGATGGACCTTACCAGAAATTAATTAATATGATAATGGGCTATCTTACCGGTGCATCACCTCACATAAAGAGTGGAGCAGTGTCTGTGCTATCAGTGCTGGTGTATAAAGATACAGATATTTGTCTTTCAATGCCCGATCTTGTTCCCTCTATTTTATCTTTGTTGCAAGGGAAAGCTTTAGAAGTTATAAAAGCTGTTTTGGGCTTTGTAAAAGTACTAGTATCCTGTTTAGAGACTAGACCTCTGCAACGGCTTCTTCCTGATATTGTCAATGCAGTTCTCCCATGGTCACCAGTTTCGAGACATCATTTCAGATCAAAGGTTACAGTCATAATAGAGATTTTGTTAAGGAAGTGCGGTACTGCATCAGTTCAGTTTGCTACACCTGATAAATACAAGGGTTTCCTGAAGGGCATTGTGGAGAGCCGCCATAACAAGAAATCTTCCAAGGAAGTTGCAGCAACCGATATAGACACGGGTCATGCAGACACTACAACCAACAGGACGGAGGATAAAAAGCGCAAGAGATTTGGTAAGCCACATGAAAAGAACGGATCAATGGAACATAGAGAGATGAAAAGGTTGAAGAGACACATTCCTAACAACCAGAGAACCAACGACCTTCATACTTCTGGCGGTGATGGATTCAAGTGGACTAATAAAGGTAGGCAATCTGATCGAGTAAAATCAAATAAAGACGAGTCAGAAAGAAGTGGAAAGACAAATAAAGAGAAATATAGTAAAGGTCCCGCAAGTGGCTTCAAGAGGAAGACGGATAGCACAAATACGAGGAAGGATGCAGCTGCAATGCCTAGACCTCACACTTCATCCAAATCATTCAAACACAAGAAGGTTGGGAGAAAAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUC173

Protein Analysis

1166

Amino Acids

128.14

Weight (kDa)

8.47

Isoelectric Point (pI)

42.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HEAT_RRP12_N PF25772 11 - 283 7.6e-101 RRP12-like, N-terminal HEAT repeats
RRP12_HEAT PF08161 354 - 655 1.9e-41 Ribosomal RNA-processing protein 12, HEAT repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011076)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23540
fragaria_vesca FvH4_6g24030 FvH4_6g24030 FvH4_6g24030 FvH4_6g24030
malus_domestica MD12G1075000.v1.1
prunus_persica Prupe.7G058600_v2.0.a1
pyrus_communis pycom12g06460 pycom14g06290
rosa_chinensis RchiOBHm_Chr3g0482611
rosa_laevigata RLG00000023336
rosa_multiflora Rmu_co8265035.1_g000001 Rmu_sc0015494.1_g000006
rosa_roxburghii Rroxscaffold_6G00399070
rosa_rugosa Rorug03G0197600
rosa_samantha Rh3AG248700 Rh3BG284200 Rh3CG281700 Rh3DG277900
rosa_wichuraiana Rw3G022460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2816
AarI CACCTGC 1 cut(s) 190
Acc36I ACCTGC 3 cut(s) 190, 1028, 1762
Acc65I GGTACC 1 cut(s) 1495
AccB1I GGYRCC 2 cut(s) 301, 1495
AccB7I CCANNNNNTGG 1 cut(s) 1111
AccII CGCG 3 cut(s) 131, 144, 233
AccIII TCCGGA 1 cut(s) 519
AclI AACGTT 2 cut(s) 549, 827
AclWI GGATC 5 cut(s) 466, 1122, 2244, 2603, 3190
AcoI YGGCCR 2 cut(s) 282, 324
AcsI RAATTY 4 cut(s) 1567, 1694, 2328, 2491
AcuI CTGAAG 6 cut(s) 386, 1062, 1134, 1370, 2007, 3056
AcvI CACGTG 1 cut(s) 1637
AfaI GTAC 8 cut(s) 810, 1204, 1497, 1800, 2164, 2580, 2844, 2989
AfiI CCNNNNNNNGG 6 cut(s) 399, 504, 519, 1111, 1578, 3383
AflII CTTAAG 1 cut(s) 1730
AflIII ACRYGT 2 cut(s) 219, 900
AgeI ACCGGT 1 cut(s) 2675
AhdI GACNNNNNGTC 2 cut(s) 220, 1831
AhlI ACTAGT 1 cut(s) 2845
AloI GAACNNNNNNTCC 2 cut(s) 903, 935
Alw21I GWGCWC 4 cut(s) 294, 842, 1189, 1636
Alw26I GTCTC 7 cut(s) 322, 473, 545, 1163, 2855, 2923, 3211
Alw44I GTGCAC 2 cut(s) 1185, 1632
AlwI GGATC 5 cut(s) 466, 1122, 2244, 2603, 3190
AlwNI CAGNNNCTG 2 cut(s) 2030, 2711
Ama87I CYCGRG 1 cut(s) 542
Aor13HI TCCGGA 1 cut(s) 519
AoxI GGCC 4 cut(s) 282, 324, 509, 1581
ApaLI GTGCAC 2 cut(s) 1185, 1632
ApeKI GCWGC 9 cut(s) 134, 194, 944, 1654, 1939, 2567, 3086, 3428, 3431
ApoI RAATTY 4 cut(s) 1567, 1694, 2328, 2491
AseI ATTAAT 3 cut(s) 1005, 2648, 2652
AsiGI ACCGGT 1 cut(s) 2675
Asp700I GAANNNNTTC 1 cut(s) 1443
Asp718I GGTACC 1 cut(s) 1495
AspLEI GCGC 4 cut(s) 144, 146, 268, 3150
AspS9I GGNCC 9 cut(s) 79, 510, 517, 668, 685, 1421, 1581, 2633, 3374
AsuC2I CCSGG 1 cut(s) 309
AsuHPI GGTGA 9 cut(s) 718, 1148, 1465, 1835, 1965, 2153, 2676, 2910, 3278
AsuNHI GCTAGC 1 cut(s) 1058
AvaI CYCGRG 1 cut(s) 542
AvaII GGWCC 7 cut(s) 79, 517, 668, 685, 1421, 2633, 3374
BaeGI GKGCMC 2 cut(s) 1189, 1636
BalI TGGCCA 1 cut(s) 284
BanI GGYRCC 2 cut(s) 301, 1495
BanII GRGCYC 1 cut(s) 842
BauI CACGAG 1 cut(s) 1629
BbrPI CACGTG 1 cut(s) 1637
BbsI GAAGAC 5 cut(s) 15, 216, 1004, 1034, 3404
Bbv12I GWGCWC 4 cut(s) 294, 842, 1189, 1636
BbvI GCAGC 9 cut(s) 121, 181, 956, 1641, 1926, 2579, 3098, 3418, 3440
BceAI ACGGC 1 cut(s) 2894
BcgI CGANNNNNNTGC 2 cut(s) 3287, 3321
BciVI GTATCC 3 cut(s) 614, 1425, 2860
BcnI CCSGG 1 cut(s) 309
BcoDI GTCTC 7 cut(s) 322, 473, 545, 1163, 2855, 2923, 3211
BcuI ACTAGT 1 cut(s) 2845
BfmI CTRYAG 2 cut(s) 879, 1622
BfoI RGCGCY 1 cut(s) 269
BfrI CTTAAG 1 cut(s) 1730
BfuAI ACCTGC 3 cut(s) 190, 1028, 1762
BfuI GTATCC 3 cut(s) 614, 1425, 2860
BglI GCCNNNNNGGC 2 cut(s) 131, 263
BglII AGATCT 2 cut(s) 1618, 2399
BmcAI AGTACT 2 cut(s) 2164, 2844
Bme1390I CCNGG 1 cut(s) 309
Bme18I GGWCC 7 cut(s) 79, 517, 668, 685, 1421, 2633, 3374
BmeRI GACNNNNNGTC 2 cut(s) 220, 1831
BmeT110I CYCGRG 1 cut(s) 542
BmgT120I GGNCC 9 cut(s) 79, 510, 517, 668, 685, 1421, 1581, 2633, 3374
BmiI GGNNCC 6 cut(s) 102, 303, 921, 1497, 2227, 3376
BmrFI CCNGG 1 cut(s) 309
BmtI GCTAGC 1 cut(s) 1062
BoxI GACNNNNGTC 1 cut(s) 3105
BpiI GAAGAC 5 cut(s) 15, 216, 1004, 1034, 3404
BplI GAGNNNNNCTC 2 cut(s) 908, 940
BpmI CTGGAG 2 cut(s) 548, 1005
BpuEI CTTGAG 7 cut(s) 473, 516, 826, 1268, 2090, 2513, 2623
BpuMI CCSGG 1 cut(s) 309
BsaAI YACGTR 2 cut(s) 901, 1637
BsaWI WCCGGW 2 cut(s) 519, 2675
BsaXI ACNNNNNCTCC 2 cut(s) 903, 933
Bsc4I CCNNNNNNNGG 6 cut(s) 399, 504, 519, 1111, 1578, 3383
Bse118I RCCGGY 1 cut(s) 2675
Bse1I ACTGG 5 cut(s) 565, 1584, 1611, 2080, 2921
Bse3DI GCAATG 2 cut(s) 2063, 3441
BseAI TCCGGA 1 cut(s) 519
BseGI GGATG 8 cut(s) 147, 227, 1222, 2008, 2018, 2554, 3430, 3455
BseLI CCNNNNNNNGG 6 cut(s) 399, 504, 519, 1111, 1578, 3383
BseMI GCAATG 2 cut(s) 2063, 3441
BseMII CTCAG 7 cut(s) 771, 1044, 1416, 1701, 1926, 1971, 2493
BseNI ACTGG 5 cut(s) 565, 1584, 1611, 2080, 2921
BsePI GCGCGC 1 cut(s) 142
BseRI GAGGAG 6 cut(s) 110, 196, 389, 392, 2351, 2573
BseSI GKGCMC 2 cut(s) 1189, 1636
BseXI GCAGC 9 cut(s) 121, 181, 956, 1641, 1926, 2579, 3098, 3418, 3440
BseYI CCCAGC 1 cut(s) 105
Bsh1236I CGCG 3 cut(s) 131, 144, 233
Bsh1285I CGRYCG 2 cut(s) 505, 1703
BshFI GGCC 4 cut(s) 284, 326, 511, 1583
BshNI GGYRCC 2 cut(s) 301, 1495
BshTI ACCGGT 1 cut(s) 2675
BsiEI CGRYCG 2 cut(s) 505, 1703
BsiHKAI GWGCWC 4 cut(s) 294, 842, 1189, 1636
BsiHKCI CYCGRG 1 cut(s) 542
BsiSI CCGG 3 cut(s) 308, 520, 2676
BslFI GGGAC 6 cut(s) 148, 220, 372, 553, 1376, 3360
BslI CCNNNNNNNGG 6 cut(s) 399, 504, 519, 1111, 1578, 3383
BsmAI GTCTC 7 cut(s) 322, 473, 545, 1163, 2855, 2923, 3211
BsmBI CGTCTC 2 cut(s) 322, 473
BsmFI GGGAC 6 cut(s) 148, 220, 372, 553, 1376, 3360
BsnI GGCC 4 cut(s) 284, 326, 511, 1583
BsoBI CYCGRG 1 cut(s) 542
Bsp1286I GDGCHC 4 cut(s) 294, 842, 1189, 1636
Bsp13I TCCGGA 1 cut(s) 519
Bsp19I CCATGG 2 cut(s) 285, 2912
BspANI GGCC 4 cut(s) 284, 326, 511, 1583
BspCNI CTCAG 7 cut(s) 770, 1045, 1417, 1702, 1927, 1972, 2492
BspEI TCCGGA 1 cut(s) 519
BspFNI CGCG 3 cut(s) 131, 144, 233
BspLI GGNNCC 6 cut(s) 102, 303, 921, 1497, 2227, 3376
BspMI ACCTGC 3 cut(s) 190, 1028, 1762
BspOI GCTAGC 1 cut(s) 1062
BspPI GGATC 5 cut(s) 466, 1122, 2244, 2603, 3190
BspT107I GGYRCC 2 cut(s) 301, 1495
BspTI CTTAAG 1 cut(s) 1730
BsrDI GCAATG 2 cut(s) 2063, 3441
BsrFI RCCGGY 1 cut(s) 2675
BsrI ACTGG 5 cut(s) 565, 1584, 1611, 2080, 2921
BssAI RCCGGY 1 cut(s) 2675
BssHII GCGCGC 1 cut(s) 142
BssSI CACGAG 1 cut(s) 1629
BssT1I CCWWGG 6 cut(s) 285, 327, 513, 2544, 2912, 3075
Bst2BI CACGAG 1 cut(s) 1629
Bst4CI ACNGT 7 cut(s) 539, 577, 606, 786, 934, 1704, 2956
Bst6I CTCTTC 2 cut(s) 362, 3209
BstAFI CTTAAG 1 cut(s) 1730
BstAPI GCANNNNNTGC 1 cut(s) 1555
BstBAI YACGTR 2 cut(s) 901, 1637
BstC8I GCNNGC 5 cut(s) 144, 334, 393, 1060, 1659
BstDSI CCRYGG 3 cut(s) 129, 285, 2912
BstEII GGTNACC 1 cut(s) 2916
BstF5I GGATG 8 cut(s) 147, 227, 1222, 2008, 2018, 2554, 3430, 3455
BstFNI CGCG 3 cut(s) 131, 144, 233
BstH2I RGCGCY 1 cut(s) 269
BstHHI GCGC 4 cut(s) 144, 146, 268, 3150
BstMAI GTCTC 7 cut(s) 322, 473, 545, 1163, 2855, 2923, 3211
BstMCI CGRYCG 2 cut(s) 505, 1703
BstNSI RCATGY 4 cut(s) 223, 628, 697, 809
BstPAI GACNNNNGTC 1 cut(s) 3105
BstPI GGTNACC 1 cut(s) 2916
BstSCI CCNGG 1 cut(s) 307
BstSFI CTRYAG 2 cut(s) 879, 1622
BstSLI GKGCMC 2 cut(s) 1189, 1636
BstUI CGCG 3 cut(s) 131, 144, 233
BstV1I GCAGC 9 cut(s) 121, 181, 956, 1641, 1926, 2579, 3098, 3418, 3440
BstV2I GAAGAC 5 cut(s) 15, 216, 1004, 1034, 3404
BstX2I RGATCY 5 cut(s) 458, 1618, 2236, 2399, 2608
BstXI CCANNNNNNTGG 1 cut(s) 1814
BstYI RGATCY 5 cut(s) 458, 1618, 2236, 2399, 2608
BsuI GTATCC 3 cut(s) 614, 1425, 2860
BsuRI GGCC 4 cut(s) 284, 326, 511, 1583
BtgI CCRYGG 3 cut(s) 129, 285, 2912
BtgZI GCGATG 1 cut(s) 2200
BtsCI GGATG 8 cut(s) 147, 227, 1222, 2008, 2018, 2554, 3430, 3455
BtsI GCAGTG 3 cut(s) 252, 1062, 2712
BtsIMutI CAGTG 7 cut(s) 252, 573, 930, 1062, 1591, 2712, 2727
BveI ACCTGC 3 cut(s) 190, 1028, 1762
Cac8I GCNNGC 5 cut(s) 144, 334, 393, 1060, 1659
CaiI CAGNNNCTG 2 cut(s) 2030, 2711
CfoI GCGC 4 cut(s) 144, 146, 268, 3150
Cfr10I RCCGGY 1 cut(s) 2675
Cfr13I GGNCC 9 cut(s) 79, 510, 517, 668, 685, 1421, 1581, 2633, 3374
Cfr42I CCGCGG 1 cut(s) 132
CseI GACGC 3 cut(s) 38, 222, 304
Csp6I GTAC 8 cut(s) 809, 1203, 1496, 1799, 2163, 2579, 2843, 2988
CspAI ACCGGT 1 cut(s) 2675
CviQI GTAC 8 cut(s) 809, 1203, 1496, 1799, 2163, 2579, 2843, 2988
DriI GACNNNNNGTC 2 cut(s) 220, 1831
EaeI YGGCCR 2 cut(s) 282, 324
Eam1104I CTCTTC 2 cut(s) 362, 3209
Eam1105I GACNNNNNGTC 2 cut(s) 220, 1831
EarI CTCTTC 2 cut(s) 362, 3209
Ecl136II GAGCTC 1 cut(s) 840
Eco130I CCWWGG 6 cut(s) 285, 327, 513, 2544, 2912, 3075
Eco24I GRGCYC 1 cut(s) 842
Eco32I GATATC 1 cut(s) 2413
Eco47I GGWCC 7 cut(s) 79, 517, 668, 685, 1421, 2633, 3374
Eco53kI GAGCTC 1 cut(s) 840
Eco57I CTGAAG 6 cut(s) 386, 1062, 1134, 1370, 2007, 3056
Eco72I CACGTG 1 cut(s) 1637
Eco88I CYCGRG 1 cut(s) 542
Eco91I GGTNACC 1 cut(s) 2916
EcoICRI GAGCTC 1 cut(s) 840
EcoO109I RGGNCCY 2 cut(s) 685, 3374
EcoO65I GGTNACC 1 cut(s) 2916
EcoRI GAATTC 1 cut(s) 2328
EcoRV GATATC 1 cut(s) 2413
EcoT14I CCWWGG 6 cut(s) 285, 327, 513, 2544, 2912, 3075
EcoT22I ATGCAT 2 cut(s) 1749, 2134
EcoT38I GRGCYC 1 cut(s) 842
ErhI CCWWGG 6 cut(s) 285, 327, 513, 2544, 2912, 3075
Esp3I CGTCTC 2 cut(s) 322, 473
FalI AAGNNNNNCTT 4 cut(s) 1132, 1164, 1724, 1756
FaqI GGGAC 6 cut(s) 148, 220, 372, 553, 1376, 3360
FauI CCCGC 4 cut(s) 325, 400, 578, 3385
FokI GGATG 8 cut(s) 134, 214, 1229, 2015, 2025, 2561, 3437, 3442
FriOI GRGCYC 1 cut(s) 842
GlaI GCGC 4 cut(s) 143, 145, 267, 3149
GsaI CCCAGC 1 cut(s) 109
GsuI CTGGAG 2 cut(s) 548, 1005
HaeII RGCGCY 1 cut(s) 269
HaeIII GGCC 4 cut(s) 284, 326, 511, 1583
HapII CCGG 3 cut(s) 308, 520, 2676
HgaI GACGC 3 cut(s) 38, 222, 304
HhaI GCGC 4 cut(s) 144, 146, 268, 3150
Hin6I GCGC 4 cut(s) 142, 144, 266, 3148
HinP1I GCGC 4 cut(s) 142, 144, 266, 3148
HindIII AAGCTT 2 cut(s) 749, 2802
HpaII CCGG 3 cut(s) 308, 520, 2676
HphI GGTGA 9 cut(s) 718, 1148, 1465, 1835, 1965, 2153, 2676, 2910, 3278
Hpy166II GTNNAC 5 cut(s) 1187, 1634, 1799, 1846, 3282
Hpy8I GTNNAC 5 cut(s) 1187, 1634, 1799, 1846, 3282
Hpy99I CGWCG 3 cut(s) 218, 325, 503
HpyAV CCTTC 6 cut(s) 698, 1158, 3031, 3260, 3415, 3472
HpyCH4III ACNGT 7 cut(s) 539, 577, 606, 786, 934, 1704, 2956
HpyCH4IV ACGT 6 cut(s) 482, 549, 827, 900, 1636, 2258
HpySE526I ACGT 6 cut(s) 482, 549, 827, 900, 1636, 2258
HspAI GCGC 4 cut(s) 142, 144, 266, 3148
Kpn2I TCCGGA 1 cut(s) 519
KpnI GGTACC 1 cut(s) 1499
KspI CCGCGG 1 cut(s) 132
Lsp1109I GCAGC 9 cut(s) 121, 181, 956, 1641, 1926, 2579, 3098, 3418, 3440
MaeII ACGT 6 cut(s) 482, 549, 827, 900, 1636, 2258
MaeIII GTNAC 8 cut(s) 483, 1466, 1535, 1720, 1823, 2026, 2916, 2950
MfeI CAATTG 1 cut(s) 1416
MflI RGATCY 5 cut(s) 458, 1618, 2236, 2399, 2608
MhlI GDGCHC 4 cut(s) 294, 842, 1189, 1636
MlsI TGGCCA 1 cut(s) 284
MluNI TGGCCA 1 cut(s) 284
MlyI GAGTC 6 cut(s) 170, 1730, 2128, 2437, 2471, 3338
MmeI TCCRAC 3 cut(s) 1501, 1700, 2094
Mox20I TGGCCA 1 cut(s) 284
Mph1103I ATGCAT 2 cut(s) 1749, 2134
MroI TCCGGA 1 cut(s) 519
MroXI GAANNNNTTC 1 cut(s) 1443
MscI TGGCCA 1 cut(s) 284
MslI CAYNNNNRTG 1 cut(s) 935
Msp20I TGGCCA 1 cut(s) 284
MspA1I CMGCKG 5 cut(s) 131, 263, 395, 2138, 3431
MspCI CTTAAG 1 cut(s) 1730
MspI CCGG 3 cut(s) 308, 520, 2676
MspR9I CCNGG 1 cut(s) 309
MunI CAATTG 1 cut(s) 1416
MvnI CGCG 3 cut(s) 131, 144, 233
NciI CCSGG 1 cut(s) 309
NcoI CCATGG 2 cut(s) 285, 2912
NheI GCTAGC 1 cut(s) 1058
NlaIV GGNNCC 6 cut(s) 102, 303, 921, 1497, 2227, 3376
NmeAIII GCCGAG 2 cut(s) 77, 184
NmuCI GTSAC 3 cut(s) 1466, 1823, 2916
NsiI ATGCAT 2 cut(s) 1749, 2134
NspI RCATGY 4 cut(s) 223, 628, 697, 809
PacI TTAATTAA 1 cut(s) 2652
PaeR7I CTCGAG 1 cut(s) 542
PaqCI CACCTGC 1 cut(s) 190
PauI GCGCGC 1 cut(s) 142
PciI ACATGT 1 cut(s) 219
PdmI GAANNNNTTC 1 cut(s) 1443
PfeI GAWTC 6 cut(s) 989, 1296, 1783, 2033, 2458, 3273
PflFI GACNNNGTC 2 cut(s) 32, 932
PflMI CCANNNNNTGG 1 cut(s) 1111
PinAI ACCGGT 1 cut(s) 2675
Ple19I CGATCG 1 cut(s) 505
PleI GAGTC 6 cut(s) 169, 1730, 2127, 2436, 2471, 3337
PmaCI CACGTG 1 cut(s) 1637
PmlI CACGTG 1 cut(s) 1637
PpsI GAGTC 6 cut(s) 169, 1730, 2127, 2436, 2471, 3337
Ppu21I YACGTR 2 cut(s) 901, 1637
PpuMI RGGWCCY 2 cut(s) 685, 3374
PscI ACATGT 1 cut(s) 219
PshAI GACNNNNGTC 1 cut(s) 3105
PshBI ATTAAT 3 cut(s) 1005, 2648, 2652
PsiI TTATAA 1 cut(s) 2816
Psp124BI GAGCTC 1 cut(s) 842
Psp1406I AACGTT 2 cut(s) 549, 827
Psp5II RGGWCCY 2 cut(s) 685, 3374
PspCI CACGTG 1 cut(s) 1637
PspEI GGTNACC 1 cut(s) 2916
PspFI CCCAGC 1 cut(s) 105
PspN4I GGNNCC 6 cut(s) 102, 303, 921, 1497, 2227, 3376
PspPI GGNCC 9 cut(s) 79, 510, 517, 668, 685, 1421, 1581, 2633, 3374
PspPPI RGGWCCY 2 cut(s) 685, 3374
PstNI CAGNNNCTG 2 cut(s) 2030, 2711
PsuI RGATCY 5 cut(s) 458, 1618, 2236, 2399, 2608
PsyI GACNNNGTC 2 cut(s) 32, 932
PteI GCGCGC 1 cut(s) 142
PvuI CGATCG 1 cut(s) 505
PvuII CAGCTG 2 cut(s) 2138, 3431
RsaI GTAC 8 cut(s) 810, 1204, 1497, 1800, 2164, 2580, 2844, 2989
RsaNI GTAC 8 cut(s) 809, 1203, 1496, 1799, 2163, 2579, 2843, 2988
RseI CAYNNNNRTG 1 cut(s) 935
SacI GAGCTC 1 cut(s) 842
SacII CCGCGG 1 cut(s) 132
Sau96I GGNCC 9 cut(s) 79, 510, 517, 668, 685, 1421, 1581, 2633, 3374
ScaI AGTACT 2 cut(s) 2164, 2844
SchI GAGTC 6 cut(s) 170, 1730, 2128, 2437, 2471, 3338
ScrFI CCNGG 1 cut(s) 309
SduI GDGCHC 4 cut(s) 294, 842, 1189, 1636
SfcI CTRYAG 2 cut(s) 879, 1622
Sfr274I CTCGAG 1 cut(s) 542
Sfr303I CCGCGG 1 cut(s) 132
SgrBI CCGCGG 1 cut(s) 132
SinI GGWCC 7 cut(s) 79, 517, 668, 685, 1421, 2633, 3374
SlaI CTCGAG 1 cut(s) 542
SmiMI CAYNNNNRTG 1 cut(s) 935
SmlI CTYRAG 9 cut(s) 488, 531, 542, 841, 1283, 1730, 2069, 2528, 2602
SmoI CTYRAG 9 cut(s) 488, 531, 542, 841, 1283, 1730, 2069, 2528, 2602
SpeI ACTAGT 1 cut(s) 2845
SspI AATATT 2 cut(s) 1321, 1756
SstI GAGCTC 1 cut(s) 842
StyD4I CCNGG 1 cut(s) 307
StyI CCWWGG 6 cut(s) 285, 327, 513, 2544, 2912, 3075
TaaI ACNGT 7 cut(s) 539, 577, 606, 786, 934, 1704, 2956
TaiI ACGT 6 cut(s) 485, 552, 830, 903, 1639, 2261
TaqI TCGA 9 cut(s) 36, 213, 347, 501, 543, 992, 1699, 2927, 3307
TatI WGTACW 6 cut(s) 808, 1202, 1798, 2162, 2578, 2842
TauI GCSGC 5 cut(s) 134, 158, 266, 511, 3057
TfiI GAWTC 6 cut(s) 989, 1296, 1783, 2033, 2458, 3273
TscAI CASTG 7 cut(s) 259, 580, 937, 1069, 1591, 2712, 2727
TseFI GTSAC 3 cut(s) 1466, 1823, 2916
TseI GCWGC 9 cut(s) 134, 194, 944, 1654, 1939, 2567, 3086, 3428, 3431
Tsp45I GTSAC 3 cut(s) 1466, 1823, 2916
TspGWI ACGGA 4 cut(s) 487, 3149, 3195, 3416
TspRI CASTG 7 cut(s) 259, 580, 937, 1069, 1591, 2712, 2727
Tth111I GACNNNGTC 2 cut(s) 32, 932
Van91I CCANNNNNTGG 1 cut(s) 1111
Vha464I CTTAAG 1 cut(s) 1730
VneI GTGCAC 2 cut(s) 1185, 1632
VpaK11BI GGWCC 7 cut(s) 79, 517, 668, 685, 1421, 2633, 3374
VspI ATTAAT 3 cut(s) 1005, 2648, 2652
XapI RAATTY 4 cut(s) 1567, 1694, 2328, 2491
XbaI TCTAGA 2 cut(s) 679, 2332
XceI RCATGY 4 cut(s) 223, 628, 697, 809
XcmI CCANNNNNNNNNTGG 1 cut(s) 2014
XhoI CTCGAG 1 cut(s) 542
XmnI GAANNNNTTC 1 cut(s) 1443
ZrmI AGTACT 2 cut(s) 2164, 2844
Zsp2I ATGCAT 2 cut(s) 1749, 2134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.