MD12G1092300.v1.1

R3H domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0017502
Physical Location & Seq
Forward (+)
11820303 .. 11823732
3430 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1092300.v1.1.491

Sequence Viewer

Length: 1059 bp
ATGGAATCCTCATCTTCTTCAGCTTCCAGCGATGGCCTTAACCTCAAAGAAGGGTCAATCGTTGACCCTTTCTTGGTCGAGGCTCTCCAGAATCCTCGCCACCGTCTCACCATTTTGCGAATGGAACTTGATATCCAGAGGTTTCTGAAAAGTCCTGATCAGCAGCTGTTTGAGTTCCAACATTTCCCTACATCCTACCTTCGACTTGCTGCACATCGTGTTTCTCAACACTATGGCTTGCAGACTATGGTTCAGGATTGGGGCTCAGATGGCCAAGGGAATAGGATTTTGGTGAGGAAAACAGCAGAAAGCAAATACCCAGCAATTCAGTTATCCGAAATACCGGCTAAGCAGTCAGAAAACAATAGACATGAGCAGATTAAAATTGTCATCAGGCCCAGGTCTGATAAAAATAATATGAATGGAGTTAATGGAGCCGGGATCAAACGAAGTCCTGTGAGAAGTGTGGAAGAGAGGAAGGAGGAGTACGATCGGGCACGGGCTCGCATCTTTAGCAGCACTAGCAGTTCTGATTCTGATGATACGCCTTTTCAGTCCCTTACAGATGCGAAAAATGCATCTTTTAGCAGGGATGAGAATGAAGGTTTTAGGAACTCTGTGGTTGATACAGAAAAGAATTTAAACGTGAAGGATCTTGGTTCTTCATCTCGAGTTGCCATTTTTAGAGATAGGGAAAAAGACCGTACTGACCCAGATTATGATCGAAGTTATGAAAGGTACGTTAGGAGTCTTCCTACCAATCAAGGATTTAACTTGGCACCTTTCAATATGCAAAAGGTTGAACCTCAGTTTTTGCAGTACGATGCTGTTTTCTCTCAGAGGCCAAGACATCAAGCTTCAATTGGTTACAGGCCTCCTTCGACCCCAGCTATGAGCCCTTTTGGTGCTGTGGGATTCAATCAGACAGGCGATGCTACGTACATGCAGTGGCCAAGTGCTTCAATGATGTATGCGCATTCATATGATCAGTTTAGGCAAGCTGTTATTCAGGCTCCATTCTGTCACCATCCCCTGAGCTTTCATTCACAAAACCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
R3H SUZ

Protein Analysis

353

Amino Acids

40.19

Weight (kDa)

8.51

Isoelectric Point (pI)

54.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
R3H PF01424 37 - 89 9.8e-09 R3H domain
SUZ PF12752 126 - 174 3.4e-11 SUZ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 975
AccB1I GGYRCC 1 cut(s) 778
AclWI GGATC 2 cut(s) 449, 660
AcoI YGGCCR 2 cut(s) 271, 950
AcsI RAATTY 1 cut(s) 637
AdeI CACNNNGTG 1 cut(s) 218
AfaI GTAC 5 cut(s) 488, 706, 740, 821, 941
AfiI CCNNNNNNNGG 1 cut(s) 73
AgsI TTSAA 5 cut(s) 787, 803, 861, 919, 963
AjnI CCWGG 1 cut(s) 398
AjuI GAANNNNNNNTTGG 2 cut(s) 272, 304
AloI GAACNNNNNNTCC 2 cut(s) 117, 149
AluBI AGCT 6 cut(s) 23, 166, 857, 890, 1001, 1038
AluI AGCT 6 cut(s) 23, 166, 857, 890, 1001, 1038
Alw26I GTCTC 1 cut(s) 110
AlwI GGATC 2 cut(s) 449, 660
AlwNI CAGNNNCTG 1 cut(s) 166
Ama87I CYCGRG 1 cut(s) 669
AoxI GGCC 6 cut(s) 34, 271, 395, 842, 872, 950
ApeKI GCWGC 3 cut(s) 163, 209, 516
ApoI RAATTY 1 cut(s) 637
AspLEI GCGC 1 cut(s) 976
AspS9I GGNCC 1 cut(s) 396
AsuC2I CCSGG 1 cut(s) 439
AsuHPI GGTGA 3 cut(s) 100, 304, 1016
AvaI CYCGRG 1 cut(s) 669
BaeGI GKGCMC 1 cut(s) 499
BalI TGGCCA 2 cut(s) 273, 952
BanI GGYRCC 1 cut(s) 778
BanII GRGCYC 3 cut(s) 266, 505, 899
BarI GAAGNNNNNNTAC 2 cut(s) 470, 502
BbsI GAAGAC 1 cut(s) 743
BbvI GCAGC 3 cut(s) 175, 196, 528
BccI CCATC 3 cut(s) 26, 263, 1035
BciT130I CCWGG 1 cut(s) 400
BclI TGATCA 2 cut(s) 157, 985
BcnI CCSGG 1 cut(s) 439
BcoDI GTCTC 1 cut(s) 110
BfaI CTAG 1 cut(s) 522
BisI GCNGC 3 cut(s) 164, 210, 517
BlpI GCTNAGC 1 cut(s) 348
BlsI GCNGC 3 cut(s) 165, 211, 518
Bme1390I CCNGG 2 cut(s) 400, 439
BmeT110I CYCGRG 1 cut(s) 669
BmgT120I GGNCC 1 cut(s) 396
BmiI GGNNCC 3 cut(s) 436, 780, 1014
BmrFI CCNGG 2 cut(s) 400, 439
BmsI GCATC 5 cut(s) 516, 556, 587, 814, 922
BpiI GAAGAC 1 cut(s) 743
BpmI CTGGAG 1 cut(s) 71
Bpu10I CCTNAGC 1 cut(s) 1034
Bpu1102I GCTNAGC 1 cut(s) 348
BpuMI CCSGG 1 cut(s) 439
BsaAI YACGTR 1 cut(s) 939
BsaBI GATNNNNATC 1 cut(s) 720
BsaJI CCNNGG 2 cut(s) 274, 398
Bsc4I CCNNNNNNNGG 1 cut(s) 73
Bse118I RCCGGY 1 cut(s) 343
Bse8I GATNNNNATC 1 cut(s) 720
BseBI CCWGG 1 cut(s) 400
BseDI CCNNGG 2 cut(s) 274, 398
BseGI GGATG 3 cut(s) 191, 598, 1027
BseJI GATNNNNATC 1 cut(s) 720
BseLI CCNNNNNNNGG 1 cut(s) 73
BseMII CTCAG 4 cut(s) 279, 821, 851, 1025
BseRI GAGGAG 1 cut(s) 497
BseSI GKGCMC 1 cut(s) 499
BseXI GCAGC 3 cut(s) 175, 196, 528
BseYI CCCAGC 2 cut(s) 319, 886
BsgI GTGCAG 1 cut(s) 195
Bsh1285I CGRYCG 1 cut(s) 493
BshFI GGCC 6 cut(s) 36, 273, 397, 844, 874, 952
BshNI GGYRCC 1 cut(s) 778
BsiEI CGRYCG 1 cut(s) 493
BsiHKCI CYCGRG 1 cut(s) 669
BsiSI CCGG 2 cut(s) 344, 438
BslFI GGGAC 1 cut(s) 541
BslI CCNNNNNNNGG 1 cut(s) 73
BsmAI GTCTC 1 cut(s) 110
BsmBI CGTCTC 1 cut(s) 110
BsmFI GGGAC 1 cut(s) 541
BsmI GAATGC 1 cut(s) 976
BsnI GGCC 6 cut(s) 36, 273, 397, 844, 874, 952
BsoBI CYCGRG 1 cut(s) 669
Bsp1286I GDGCHC 4 cut(s) 266, 499, 505, 899
Bsp143I GATC 6 cut(s) 157, 441, 490, 652, 721, 985
Bsp1720I GCTNAGC 1 cut(s) 348
BspANI GGCC 6 cut(s) 36, 273, 397, 844, 874, 952
BspCNI CTCAG 4 cut(s) 278, 820, 850, 1026
BspLI GGNNCC 3 cut(s) 436, 780, 1014
BspPI GGATC 2 cut(s) 449, 660
BspT107I GGYRCC 1 cut(s) 778
BsrFI RCCGGY 1 cut(s) 343
BssAI RCCGGY 1 cut(s) 343
BssECI CCNNGG 2 cut(s) 274, 398
BssMI GATC 6 cut(s) 157, 441, 490, 652, 721, 985
BssT1I CCWWGG 1 cut(s) 274
Bst2UI CCWGG 1 cut(s) 400
Bst4CI ACNGT 2 cut(s) 104, 704
Bst6I CTCTTC 1 cut(s) 465
BstBAI YACGTR 1 cut(s) 939
BstC8I GCNNGC 3 cut(s) 239, 505, 999
BstDEI CTNAG 5 cut(s) 265, 348, 807, 837, 1034
BstF5I GGATG 3 cut(s) 191, 598, 1027
BstHHI GCGC 1 cut(s) 976
BstKTI GATC 6 cut(s) 160, 444, 493, 655, 724, 988
BstMAI GTCTC 1 cut(s) 110
BstMBI GATC 6 cut(s) 157, 441, 490, 652, 721, 985
BstMCI CGRYCG 1 cut(s) 493
BstMWI GCNNNNNNNGC 4 cut(s) 270, 513, 522, 575
BstNI CCWGG 1 cut(s) 400
BstNSI RCATGY 1 cut(s) 946
BstSCI CCNGG 2 cut(s) 398, 437
BstSLI GKGCMC 1 cut(s) 499
BstSNI TACGTA 1 cut(s) 939
BstV1I GCAGC 3 cut(s) 175, 196, 528
BstV2I GAAGAC 1 cut(s) 743
BstX2I RGATCY 1 cut(s) 652
BstYI RGATCY 1 cut(s) 652
BsuRI GGCC 6 cut(s) 36, 273, 397, 844, 874, 952
BtgZI GCGATG 2 cut(s) 45, 945
BtsCI GGATG 3 cut(s) 191, 598, 1027
BtsI GCAGTG 1 cut(s) 953
BtsIMutI CAGTG 1 cut(s) 953
Cac8I GCNNGC 3 cut(s) 239, 505, 999
CaiI CAGNNNCTG 1 cut(s) 166
CfoI GCGC 1 cut(s) 976
Cfr10I RCCGGY 1 cut(s) 343
Cfr13I GGNCC 1 cut(s) 396
Csp6I GTAC 5 cut(s) 487, 705, 739, 820, 940
CviAII CATG 2 cut(s) 371, 943
CviQI GTAC 5 cut(s) 487, 705, 739, 820, 940
DdeI CTNAG 5 cut(s) 265, 348, 807, 837, 1034
DpnI GATC 6 cut(s) 159, 443, 492, 654, 723, 987
DpnII GATC 6 cut(s) 157, 441, 490, 652, 721, 985
DraI TTTAAA 1 cut(s) 642
DraIII CACNNNGTG 1 cut(s) 218
EaeI YGGCCR 2 cut(s) 271, 950
Eam1104I CTCTTC 1 cut(s) 465
EarI CTCTTC 1 cut(s) 465
Eco105I TACGTA 1 cut(s) 939
Eco130I CCWWGG 1 cut(s) 274
Eco147I AGGCCT 1 cut(s) 874
Eco24I GRGCYC 3 cut(s) 266, 505, 899
Eco32I GATATC 1 cut(s) 133
Eco88I CYCGRG 1 cut(s) 669
EcoRII CCWGG 1 cut(s) 398
EcoRV GATATC 1 cut(s) 133
EcoT14I CCWWGG 1 cut(s) 274
EcoT22I ATGCAT 1 cut(s) 580
EcoT38I GRGCYC 3 cut(s) 266, 505, 899
ErhI CCWWGG 1 cut(s) 274
Esp3I CGTCTC 1 cut(s) 110
FaeI CATG 2 cut(s) 374, 946
FaqI GGGAC 1 cut(s) 541
FatI CATG 2 cut(s) 370, 942
FauNDI CATATG 1 cut(s) 982
FbaI TGATCA 2 cut(s) 157, 985
Fnu4HI GCNGC 3 cut(s) 164, 210, 517
FokI GGATG 3 cut(s) 178, 605, 1014
FriOI GRGCYC 3 cut(s) 266, 505, 899
Fsp4HI GCNGC 3 cut(s) 164, 210, 517
FspAI RTGCGCAY 1 cut(s) 975
FspBI CTAG 1 cut(s) 522
FspI TGCGCA 1 cut(s) 975
GlaI GCGC 1 cut(s) 975
GluI GCNGC 3 cut(s) 164, 210, 517
GsaI CCCAGC 2 cut(s) 323, 890
GsuI CTGGAG 1 cut(s) 71
HaeIII GGCC 6 cut(s) 36, 273, 397, 844, 874, 952
HapII CCGG 2 cut(s) 344, 438
HhaI GCGC 1 cut(s) 976
Hin1II CATG 2 cut(s) 374, 946
Hin6I GCGC 1 cut(s) 974
HinP1I GCGC 1 cut(s) 974
HincII GTYRAC 1 cut(s) 64
HindII GTYRAC 1 cut(s) 64
HindIII AAGCTT 1 cut(s) 855
HinfI GANTC 5 cut(s) 5, 91, 533, 748, 915
HpaII CCGG 2 cut(s) 344, 438
HphI GGTGA 3 cut(s) 100, 304, 1016
Hpy166II GTNNAC 1 cut(s) 64
Hpy188I TCNGA 9 cut(s) 147, 268, 337, 358, 406, 532, 538, 840, 924
Hpy188III TCNNGA 5 cut(s) 88, 136, 155, 254, 669
Hpy8I GTNNAC 1 cut(s) 64
HpyAV CCTTC 6 cut(s) 44, 209, 472, 596, 643, 888
HpyCH4III ACNGT 2 cut(s) 104, 704
HpyCH4IV ACGT 3 cut(s) 645, 741, 938
HpyCH4V TGCA 6 cut(s) 212, 241, 578, 793, 817, 946
HpyF10VI GCNNNNNNNGC 4 cut(s) 270, 513, 522, 575
HpyF3I CTNAG 5 cut(s) 265, 348, 807, 837, 1034
HpySE526I ACGT 3 cut(s) 645, 741, 938
Hsp92II CATG 2 cut(s) 374, 946
HspAI GCGC 1 cut(s) 974
Ksp22I TGATCA 2 cut(s) 157, 985
Kzo9I GATC 6 cut(s) 157, 441, 490, 652, 721, 985
LmnI GCTCC 2 cut(s) 434, 1018
Lsp1109I GCAGC 3 cut(s) 175, 196, 528
LweI GCATC 5 cut(s) 516, 556, 587, 814, 922
MaeI CTAG 1 cut(s) 522
MaeII ACGT 3 cut(s) 645, 741, 938
MaeIII GTNAC 2 cut(s) 866, 1022
MalI GATC 6 cut(s) 159, 443, 492, 654, 723, 987
MboI GATC 6 cut(s) 157, 441, 490, 652, 721, 985
MboII GAAGA 5 cut(s) 6, 9, 482, 654, 743
MfeI CAATTG 1 cut(s) 861
MflI RGATCY 1 cut(s) 652
MhlI GDGCHC 4 cut(s) 266, 499, 505, 899
MlsI TGGCCA 2 cut(s) 273, 952
MluCI AATT 4 cut(s) 324, 384, 637, 861
MluNI TGGCCA 2 cut(s) 273, 952
MlyI GAGTC 1 cut(s) 757
MmeI TCCRAC 1 cut(s) 202
Mox20I TGGCCA 2 cut(s) 273, 952
Mph1103I ATGCAT 1 cut(s) 580
MscI TGGCCA 2 cut(s) 273, 952
MseI TTAA 6 cut(s) 39, 381, 429, 641, 771, 1057
MslI CAYNNNNRTG 1 cut(s) 981
Msp20I TGGCCA 2 cut(s) 273, 952
MspA1I CMGCKG 1 cut(s) 166
MspI CCGG 2 cut(s) 344, 438
MspR9I CCNGG 2 cut(s) 400, 439
MunI CAATTG 1 cut(s) 861
Mva1269I GAATGC 1 cut(s) 976
MvaI CCWGG 1 cut(s) 400
MwoI GCNNNNNNNGC 4 cut(s) 270, 513, 522, 575
NciI CCSGG 1 cut(s) 439
NdeI CATATG 1 cut(s) 982
NdeII GATC 6 cut(s) 157, 441, 490, 652, 721, 985
NlaIII CATG 2 cut(s) 374, 946
NlaIV GGNNCC 3 cut(s) 436, 780, 1014
NmuCI GTSAC 1 cut(s) 1022
NsbI TGCGCA 1 cut(s) 975
NsiI ATGCAT 1 cut(s) 580
NspI RCATGY 1 cut(s) 946
PaeR7I CTCGAG 1 cut(s) 669
PceI AGGCCT 1 cut(s) 874
PctI GAATGC 1 cut(s) 976
PfeI GAWTC 4 cut(s) 5, 91, 533, 915
PkrI GCNGC 3 cut(s) 165, 211, 518
Ple19I CGATCG 1 cut(s) 493
PleI GAGTC 1 cut(s) 756
PpsI GAGTC 1 cut(s) 756
Ppu21I YACGTR 1 cut(s) 939
Psp6I CCWGG 1 cut(s) 398
PspFI CCCAGC 2 cut(s) 319, 886
PspGI CCWGG 1 cut(s) 398
PspN4I GGNNCC 3 cut(s) 436, 780, 1014
PspPI GGNCC 1 cut(s) 396
PstNI CAGNNNCTG 1 cut(s) 166
PsuI RGATCY 1 cut(s) 652
PvuI CGATCG 1 cut(s) 493
PvuII CAGCTG 1 cut(s) 166
RsaI GTAC 5 cut(s) 488, 706, 740, 821, 941
RsaNI GTAC 5 cut(s) 487, 705, 739, 820, 940
RseI CAYNNNNRTG 1 cut(s) 981
SaqAI TTAA 6 cut(s) 39, 381, 429, 641, 771, 1057
SatI GCNGC 3 cut(s) 164, 210, 517
Sau3AI GATC 6 cut(s) 157, 441, 490, 652, 721, 985
Sau96I GGNCC 1 cut(s) 396
SchI GAGTC 1 cut(s) 757
ScrFI CCNGG 2 cut(s) 400, 439
SduI GDGCHC 4 cut(s) 266, 499, 505, 899
SfaNI GCATC 5 cut(s) 516, 556, 587, 814, 922
Sfr274I CTCGAG 1 cut(s) 669
SlaI CTCGAG 1 cut(s) 669
SmiMI CAYNNNNRTG 1 cut(s) 981
SmlI CTYRAG 1 cut(s) 669
SmoI CTYRAG 1 cut(s) 669
SnaBI TACGTA 1 cut(s) 939
Sse9I AATT 4 cut(s) 324, 384, 637, 861
SseBI AGGCCT 1 cut(s) 874
SspMI CTAG 1 cut(s) 522
StuI AGGCCT 1 cut(s) 874
StyD4I CCNGG 2 cut(s) 398, 437
StyI CCWWGG 1 cut(s) 274
TaaI ACNGT 2 cut(s) 104, 704
TaiI ACGT 3 cut(s) 648, 744, 941
TaqI TCGA 5 cut(s) 78, 202, 670, 724, 881
TasI AATT 4 cut(s) 324, 384, 637, 861
TfiI GAWTC 4 cut(s) 5, 91, 533, 915
Tru1I TTAA 6 cut(s) 39, 381, 429, 641, 771, 1057
Tru9I TTAA 6 cut(s) 39, 381, 429, 641, 771, 1057
TscAI CASTG 1 cut(s) 953
TseFI GTSAC 1 cut(s) 1022
TseI GCWGC 3 cut(s) 163, 209, 516
Tsp45I GTSAC 1 cut(s) 1022
TspDTI ATGAA 6 cut(s) 434, 615, 654, 747, 969, 1031
TspRI CASTG 1 cut(s) 953
XapI RAATTY 1 cut(s) 637
XceI RCATGY 1 cut(s) 946
XcmI CCANNNNNNNNNTGG 1 cut(s) 118
XhoI CTCGAG 1 cut(s) 669
XspI CTAG 1 cut(s) 522
Zsp2I ATGCAT 1 cut(s) 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.