MD12G1100400.v1.1

Protein of unknown function, DUF617

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0016130
Physical Location & Seq
Reverse (-)
15608037 .. 15608795
759 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1100400.v1.1.491

Sequence Viewer

Length: 759 bp
ATGGGGGAGCCGAAATCTCCAATATCACAAGACCATCAGACGACGGCAGCAGCCACAACAATATCCTCGTCATCTACAGTCCCGCCACCACCGCCACTTCCCCGCCAGCAATCCTCCCTTCTCGAACCATGCCATAAAAAGAAAAGCAAAACCAAAGTCTTCCGCGTCTTTCGCTCTGTATTCCGATCCTTCCCCATCATCACCCCCACCTGCAAGATGCCCTCACTCCCGATGGGCAGGCTCGACTCTAGCCGGGCCATCTCCTCTGGCACTCGGGTCAGTGGCACGCTGTTTGGGTACCGAAAGGGACGTGTGTCTCTTTCGGTACAAGAAACCCCTAGGTGTCTTCCGAGCCTAGTGGTGGAGATGGCCATGCAGACAAATGTGCTGCAGAAGGAGATGGGGTCAGGGATGGTCAGAATCGCGTTGGAATGCGAAAAGAGAGCGGACAAGGAGAAGACGAGGCTTATTGACGAGCCGGTGTGGACCATATACTGCAATGGGAAGAAGACAGGGTATGGAATGAAGAGGGAGGCCACAGAGGAGGACTTGAACGTTATGGAACTTCTCAAGGCAGTGACTATGGGCGCAGGTGTGTTGCCCGGGAAGTCTGAGGTAGAGGGTCCGGATGGCGAAATGGCGTATATGCGGGCCCTCTTCGAACGTGTTGTTGGCTCAAAAGACTCGGAAACTTTGTACATGTTGAGTCCTGAGGGGAATAATGGGCCCGAGCTCAGTATTTTCTTTGTAAGGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF617

Protein Analysis

253

Amino Acids

27.75

Weight (kDa)

9.28

Isoelectric Point (pI)

51.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF617 PF04759 93 - 251 4.3e-71 Protein of unknown function, DUF617
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016130)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21990
fragaria_vesca FvH4_1g22210
malus_domestica MD12G1100400.v1.1
prunus_persica Prupe.6G173500_v2.0.a1
pyrus_communis pycom12g09880
rosa_chinensis RchiOBHm_Chr2g0116351
rosa_laevigata RLG00000018261
rosa_multiflora Rmu_sc0015281.1_g000003
rosa_roxburghii Rroxscaffold_2G00127300
rosa_rugosa Rorug02G0205800
rosa_samantha Rh2AG261300 Rh2BG272700 Rh2CG267700 Rh2DG269500
rosa_wichuraiana Rw2G020490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 218, 581
Acc36I ACCTGC 2 cut(s) 218, 581
Acc65I GGTACC 1 cut(s) 297
AccB1I GGYRCC 1 cut(s) 297
AccBSI CCGCTC 1 cut(s) 446
AccII CGCG 2 cut(s) 165, 425
AccIII TCCGGA 1 cut(s) 625
AciI CCGC 6 cut(s) 83, 92, 103, 163, 446, 649
AclI AACGTT 1 cut(s) 555
AclWI GGATC 1 cut(s) 180
AcoI YGGCCR 1 cut(s) 369
AfaI GTAC 3 cut(s) 299, 327, 698
AfiI CCNNNNNNNGG 1 cut(s) 361
AflIII ACRYGT 3 cut(s) 310, 664, 699
AgsI TTSAA 1 cut(s) 553
AjiI CACGTC 1 cut(s) 311
AjuI GAANNNNNNNTTGG 2 cut(s) 654, 686
AluBI AGCT 1 cut(s) 733
AluI AGCT 1 cut(s) 733
Alw21I GWGCWC 1 cut(s) 735
Alw26I GTCTC 1 cut(s) 321
AlwI GGATC 1 cut(s) 180
Ama87I CYCGRG 3 cut(s) 273, 602, 728
Aor13HI TCCGGA 1 cut(s) 625
AoxI GGCC 5 cut(s) 255, 369, 534, 651, 725
ApaI GGGCCC 2 cut(s) 655, 729
ApeKI GCWGC 3 cut(s) 47, 50, 388
ArsI GACNNNNNNTTYG 2 cut(s) 141, 173
Asp718I GGTACC 1 cut(s) 297
AspA2I CCTAGG 1 cut(s) 338
AspLEI GCGC 1 cut(s) 590
AspS9I GGNCC 7 cut(s) 255, 486, 623, 651, 652, 725, 726
AsuC2I CCSGG 3 cut(s) 254, 603, 604
AsuHPI GGTGA 1 cut(s) 193
AsuII TTCGAA 1 cut(s) 660
AvaI CYCGRG 3 cut(s) 273, 602, 728
AvaII GGWCC 2 cut(s) 486, 623
AvrII CCTAGG 1 cut(s) 338
AxyI CCTNAGG 1 cut(s) 711
BaeGI GKGCMC 2 cut(s) 655, 729
BalI TGGCCA 1 cut(s) 371
BanI GGYRCC 1 cut(s) 297
BanII GRGCYC 3 cut(s) 655, 729, 735
BbsI GAAGAC 4 cut(s) 151, 338, 464, 515
Bbv12I GWGCWC 1 cut(s) 735
BbvI GCAGC 3 cut(s) 59, 62, 375
BccI CCATC 8 cut(s) 42, 203, 226, 266, 361, 394, 406, 623
BceAI ACGGC 1 cut(s) 60
BcnI CCSGG 3 cut(s) 254, 603, 604
BcoDI GTCTC 1 cut(s) 321
BfaI CTAG 3 cut(s) 249, 339, 356
BfmI CTRYAG 2 cut(s) 75, 389
BfuAI ACCTGC 2 cut(s) 218, 581
BisI GCNGC 3 cut(s) 48, 51, 389
BlnI CCTAGG 1 cut(s) 338
BlsI GCNGC 3 cut(s) 49, 52, 390
Bme1390I CCNGG 3 cut(s) 254, 603, 604
Bme18I GGWCC 2 cut(s) 486, 623
BmeT110I CYCGRG 3 cut(s) 273, 602, 728
BmgBI CACGTC 1 cut(s) 311
BmgT120I GGNCC 7 cut(s) 255, 486, 623, 651, 652, 725, 726
BmiI GGNNCC 5 cut(s) 9, 299, 624, 653, 727
BmrFI CCNGG 3 cut(s) 254, 603, 604
BmsI GCATC 1 cut(s) 207
BoxI GACNNNNGTC 1 cut(s) 313
BpiI GAAGAC 4 cut(s) 151, 338, 464, 515
Bpu14I TTCGAA 1 cut(s) 660
BpuEI CTTGAG 1 cut(s) 554
BpuMI CCSGG 3 cut(s) 254, 603, 604
BsaJI CCNNGG 2 cut(s) 338, 602
BsaWI WCCGGW 1 cut(s) 625
BsaXI ACNNNNNCTCC 2 cut(s) 389, 419
Bsc4I CCNNNNNNNGG 1 cut(s) 361
Bse118I RCCGGY 1 cut(s) 478
Bse21I CCTNAGG 1 cut(s) 711
Bse3DI GCAATG 1 cut(s) 505
BseAI TCCGGA 1 cut(s) 625
BseDI CCNNGG 2 cut(s) 338, 602
BseGI GGATG 2 cut(s) 417, 634
BseLI CCNNNNNNNGG 1 cut(s) 361
BseMI GCAATG 1 cut(s) 505
BseMII CTCAG 3 cut(s) 603, 702, 748
BseRI GAGGAG 2 cut(s) 253, 557
BseSI GKGCMC 2 cut(s) 655, 729
BseXI GCAGC 3 cut(s) 59, 62, 375
Bsh1236I CGCG 2 cut(s) 165, 425
BshFI GGCC 5 cut(s) 257, 371, 536, 653, 727
BshNI GGYRCC 1 cut(s) 297
BsiHKAI GWGCWC 1 cut(s) 735
BsiHKCI CYCGRG 3 cut(s) 273, 602, 728
BsiSI CCGG 4 cut(s) 253, 479, 603, 626
BslFI GGGAC 2 cut(s) 65, 321
BslI CCNNNNNNNGG 1 cut(s) 361
BsmAI GTCTC 1 cut(s) 321
BsmFI GGGAC 2 cut(s) 65, 321
BsmI GAATGC 1 cut(s) 437
BsnI GGCC 5 cut(s) 257, 371, 536, 653, 727
BsoBI CYCGRG 3 cut(s) 273, 602, 728
Bsp119I TTCGAA 1 cut(s) 660
Bsp120I GGGCCC 2 cut(s) 651, 725
Bsp1286I GDGCHC 3 cut(s) 655, 729, 735
Bsp13I TCCGGA 1 cut(s) 625
Bsp1407I TGTACA 1 cut(s) 696
Bsp143I GATC 1 cut(s) 185
BspACI CCGC 6 cut(s) 83, 92, 103, 163, 446, 649
BspANI GGCC 5 cut(s) 257, 371, 536, 653, 727
BspCNI CTCAG 3 cut(s) 604, 703, 747
BspEI TCCGGA 1 cut(s) 625
BspFNI CGCG 2 cut(s) 165, 425
BspLI GGNNCC 5 cut(s) 9, 299, 624, 653, 727
BspMAI CTGCAG 1 cut(s) 393
BspMI ACCTGC 2 cut(s) 218, 581
BspPI GGATC 1 cut(s) 180
BspT104I TTCGAA 1 cut(s) 660
BspT107I GGYRCC 1 cut(s) 297
BsrBI CCGCTC 1 cut(s) 446
BsrDI GCAATG 1 cut(s) 505
BsrFI RCCGGY 1 cut(s) 478
BsrGI TGTACA 1 cut(s) 696
BssAI RCCGGY 1 cut(s) 478
BssECI CCNNGG 2 cut(s) 338, 602
BssMI GATC 1 cut(s) 185
BssT1I CCWWGG 1 cut(s) 338
Bst4CI ACNGT 1 cut(s) 79
Bst6I CTCTTC 2 cut(s) 521, 662
BstAUI TGTACA 1 cut(s) 696
BstBI TTCGAA 1 cut(s) 660
BstC8I GCNNGC 4 cut(s) 107, 239, 287, 651
BstDEI CTNAG 3 cut(s) 612, 711, 734
BstF5I GGATG 2 cut(s) 417, 634
BstFNI CGCG 2 cut(s) 165, 425
BstHHI GCGC 1 cut(s) 590
BstKTI GATC 1 cut(s) 188
BstMAI GTCTC 1 cut(s) 321
BstMBI GATC 1 cut(s) 185
BstMWI GCNNNNNNNGC 2 cut(s) 91, 171
BstNSI RCATGY 1 cut(s) 703
BstPAI GACNNNNGTC 1 cut(s) 313
BstSCI CCNGG 3 cut(s) 252, 601, 602
BstSFI CTRYAG 2 cut(s) 75, 389
BstSLI GKGCMC 2 cut(s) 655, 729
BstUI CGCG 2 cut(s) 165, 425
BstV1I GCAGC 3 cut(s) 59, 62, 375
BstV2I GAAGAC 4 cut(s) 151, 338, 464, 515
Bsu36I CCTNAGG 1 cut(s) 711
BsuRI GGCC 5 cut(s) 257, 371, 536, 653, 727
BtrI CACGTC 1 cut(s) 311
BtsCI GGATG 2 cut(s) 417, 634
BtsI GCAGTG 1 cut(s) 582
BtsIMutI CAGTG 2 cut(s) 286, 582
BveI ACCTGC 2 cut(s) 218, 581
Cac8I GCNNGC 4 cut(s) 107, 239, 287, 651
CfoI GCGC 1 cut(s) 590
Cfr10I RCCGGY 1 cut(s) 478
Cfr13I GGNCC 7 cut(s) 255, 486, 623, 651, 652, 725, 726
Cfr9I CCCGGG 1 cut(s) 602
CseI GACGC 1 cut(s) 154
Csp6I GTAC 3 cut(s) 298, 326, 697
CviAII CATG 3 cut(s) 129, 373, 700
CviQI GTAC 3 cut(s) 298, 326, 697
DdeI CTNAG 3 cut(s) 612, 711, 734
DpnI GATC 1 cut(s) 187
DpnII GATC 1 cut(s) 185
EaeI YGGCCR 1 cut(s) 369
Eam1104I CTCTTC 2 cut(s) 521, 662
EarI CTCTTC 2 cut(s) 521, 662
Ecl136II GAGCTC 1 cut(s) 733
Eco130I CCWWGG 1 cut(s) 338
Eco24I GRGCYC 3 cut(s) 655, 729, 735
Eco47I GGWCC 2 cut(s) 486, 623
Eco53kI GAGCTC 1 cut(s) 733
Eco81I CCTNAGG 1 cut(s) 711
Eco88I CYCGRG 3 cut(s) 273, 602, 728
EcoICRI GAGCTC 1 cut(s) 733
EcoO109I RGGNCCY 1 cut(s) 652
EcoT14I CCWWGG 1 cut(s) 338
EcoT38I GRGCYC 3 cut(s) 655, 729, 735
ErhI CCWWGG 1 cut(s) 338
FaeI CATG 3 cut(s) 132, 376, 703
FaqI GGGAC 2 cut(s) 65, 321
FatI CATG 3 cut(s) 128, 372, 699
FauI CCCGC 3 cut(s) 90, 110, 642
Fnu4HI GCNGC 3 cut(s) 48, 51, 389
FokI GGATG 2 cut(s) 424, 641
FriOI GRGCYC 3 cut(s) 655, 729, 735
Fsp4HI GCNGC 3 cut(s) 48, 51, 389
FspBI CTAG 3 cut(s) 249, 339, 356
GlaI GCGC 1 cut(s) 589
GluI GCNGC 3 cut(s) 48, 51, 389
HaeIII GGCC 5 cut(s) 257, 371, 536, 653, 727
HapII CCGG 4 cut(s) 253, 479, 603, 626
HgaI GACGC 1 cut(s) 154
HhaI GCGC 1 cut(s) 590
Hin1II CATG 3 cut(s) 132, 376, 703
Hin6I GCGC 1 cut(s) 588
HinP1I GCGC 1 cut(s) 588
HinfI GANTC 4 cut(s) 245, 420, 683, 706
HpaII CCGG 4 cut(s) 253, 479, 603, 626
HphI GGTGA 1 cut(s) 193
Hpy166II GTNNAC 1 cut(s) 486
Hpy188I TCNGA 6 cut(s) 39, 185, 351, 419, 613, 688
Hpy188III TCNNGA 4 cut(s) 122, 229, 626, 710
Hpy8I GTNNAC 1 cut(s) 486
Hpy99I CGWCG 1 cut(s) 46
HpyAV CCTTC 3 cut(s) 128, 199, 388
HpyCH4III ACNGT 1 cut(s) 79
HpyCH4IV ACGT 3 cut(s) 310, 555, 664
HpyCH4V TGCA 4 cut(s) 213, 376, 391, 498
HpyF10VI GCNNNNNNNGC 2 cut(s) 91, 171
HpyF3I CTNAG 3 cut(s) 612, 711, 734
HpySE526I ACGT 3 cut(s) 310, 555, 664
Hsp92II CATG 3 cut(s) 132, 376, 703
HspAI GCGC 1 cut(s) 588
Kpn2I TCCGGA 1 cut(s) 625
KpnI GGTACC 1 cut(s) 301
Kzo9I GATC 1 cut(s) 185
LmnI GCTCC 1 cut(s) 7
Lsp1109I GCAGC 3 cut(s) 59, 62, 375
LweI GCATC 1 cut(s) 207
MaeI CTAG 3 cut(s) 249, 339, 356
MaeII ACGT 3 cut(s) 310, 555, 664
MaeIII GTNAC 1 cut(s) 577
MalI GATC 1 cut(s) 187
MbiI CCGCTC 1 cut(s) 446
MboI GATC 1 cut(s) 185
MboII GAAGA 7 cut(s) 151, 338, 469, 517, 520, 538, 649
MhlI GDGCHC 3 cut(s) 655, 729, 735
MlsI TGGCCA 1 cut(s) 371
MluNI TGGCCA 1 cut(s) 371
MlyI GAGTC 3 cut(s) 239, 677, 715
MmeI TCCRAC 1 cut(s) 408
Mox20I TGGCCA 1 cut(s) 371
MroI TCCGGA 1 cut(s) 625
MscI TGGCCA 1 cut(s) 371
Msp20I TGGCCA 1 cut(s) 371
MspI CCGG 4 cut(s) 253, 479, 603, 626
MspR9I CCNGG 3 cut(s) 254, 603, 604
Mva1269I GAATGC 1 cut(s) 437
MvnI CGCG 2 cut(s) 165, 425
MwoI GCNNNNNNNGC 2 cut(s) 91, 171
NciI CCSGG 3 cut(s) 254, 603, 604
NdeII GATC 1 cut(s) 185
NlaIII CATG 3 cut(s) 132, 376, 703
NlaIV GGNNCC 5 cut(s) 9, 299, 624, 653, 727
NmuCI GTSAC 1 cut(s) 577
NspI RCATGY 1 cut(s) 703
NspV TTCGAA 1 cut(s) 660
PaqCI CACCTGC 2 cut(s) 218, 581
PciI ACATGT 1 cut(s) 699
PctI GAATGC 1 cut(s) 437
PfeI GAWTC 1 cut(s) 420
PkrI GCNGC 3 cut(s) 49, 52, 390
PleI GAGTC 3 cut(s) 239, 677, 714
PpsI GAGTC 3 cut(s) 239, 677, 714
PscI ACATGT 1 cut(s) 699
PshAI GACNNNNGTC 1 cut(s) 313
Psp124BI GAGCTC 1 cut(s) 735
Psp1406I AACGTT 1 cut(s) 555
PspN4I GGNNCC 5 cut(s) 9, 299, 624, 653, 727
PspOMI GGGCCC 2 cut(s) 651, 725
PspPI GGNCC 7 cut(s) 255, 486, 623, 651, 652, 725, 726
PstI CTGCAG 1 cut(s) 393
RsaI GTAC 3 cut(s) 299, 327, 698
RsaNI GTAC 3 cut(s) 298, 326, 697
SacI GAGCTC 1 cut(s) 735
SatI GCNGC 3 cut(s) 48, 51, 389
Sau3AI GATC 1 cut(s) 185
Sau96I GGNCC 7 cut(s) 255, 486, 623, 651, 652, 725, 726
SchI GAGTC 3 cut(s) 239, 677, 715
ScrFI CCNGG 3 cut(s) 254, 603, 604
SduI GDGCHC 3 cut(s) 655, 729, 735
SetI ASST 8 cut(s) 212, 313, 344, 558, 595, 618, 667, 735
SfaNI GCATC 1 cut(s) 207
SfcI CTRYAG 2 cut(s) 75, 389
SfuI TTCGAA 1 cut(s) 660
SinI GGWCC 2 cut(s) 486, 623
SmaI CCCGGG 1 cut(s) 604
SmlI CTYRAG 1 cut(s) 569
SmoI CTYRAG 1 cut(s) 569
SsiI CCGC 6 cut(s) 83, 92, 103, 163, 446, 649
SspMI CTAG 3 cut(s) 249, 339, 356
SstI GAGCTC 1 cut(s) 735
StyD4I CCNGG 3 cut(s) 252, 601, 602
StyI CCWWGG 1 cut(s) 338
TaaI ACNGT 1 cut(s) 79
TaiI ACGT 3 cut(s) 313, 558, 667
TaqI TCGA 3 cut(s) 123, 243, 660
TatI WGTACW 1 cut(s) 696
TfiI GAWTC 1 cut(s) 420
TscAI CASTG 2 cut(s) 286, 582
TseFI GTSAC 1 cut(s) 577
TseI GCWGC 3 cut(s) 47, 50, 388
Tsp45I GTSAC 1 cut(s) 577
TspDTI ATGAA 1 cut(s) 539
TspMI CCCGGG 1 cut(s) 602
TspRI CASTG 2 cut(s) 286, 582
VpaK11BI GGWCC 2 cut(s) 486, 623
XceI RCATGY 1 cut(s) 703
XmaI CCCGGG 1 cut(s) 602
XmaJI CCTAGG 1 cut(s) 338
XspI CTAG 3 cut(s) 249, 339, 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.