Rroxscaffold_2G00127300

Protein of unknown function, DUF617

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0016130
Physical Location & Seq
Reverse (-)
62756443 .. 62757207
765 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00127300.1

Sequence Viewer

Length: 765 bp
ATGGGGGAGCCAACAAAATCTGTATCAAAAGAACAGGACCGTCCGTCAGCATCCTCCTCCACCCAGACAACCAACGTACCACCGGCGCCGCCACTTCCCAATCCCAGCACACACTCGAGCCTCCTCCAGCCCTCCATTAAAAAGAAAAACAAAACCAAAGTCTTCCGCGTCTTCAGATCCGTCATCCGGTCCTTTCCCATCATCACACCCACAGCATGCAAAATGCCGTCCCTCCCCGGCGGCAAGCTCGATTCCAACCGCACCGTTACTTCTGGTGTCCGAGTCAGCGGCACGCTGTTCGGGTACCGGAAGGGCCGGGTGAGCCTGTCAATACAGGAAAGCTCTAAGACTCTCCCCAGCTTCATTGTCGAGCTAGCCATGCAAACCAACGTGCTGCAGAAGGAGATGAGCTCAGGGATGGTGAGGATCGCTCTCGAGTGCGAGAAGAGAGGTGAAAAAGATAAGACGAAGCTCATGGACGAGCCTGTCTGGACTATGTTTTGTAATGGGAAGAAGACGGGTTATGCGGTTAAGAGGGAGGCCACGGAGGAGGACTTGAATGTCATGGAGCTGCTCAAGGCTGTGACTATGGGAGCGGGCGTGTTGCCCGGGAAGTCAGACGTTGAGGGCCCGGATGGCGAGATGGCGTACATGCGGGCCCTCTTCGAGCGGGTTGTAGGTTCGAAGGATTCCGAAACGTTGTATATGTTGAGCCCGGAAGGGAATAATGGGCCTGAGCTCAGTATTTTCTTTGTAAGGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF617

Protein Analysis

254

Amino Acids

27.81

Weight (kDa)

9.45

Isoelectric Point (pI)

49.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF617 PF04759 95 - 253 3.7e-72 Protein of unknown function, DUF617
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016130)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21990
fragaria_vesca FvH4_1g22210
malus_domestica MD12G1100400.v1.1
prunus_persica Prupe.6G173500_v2.0.a1
pyrus_communis pycom12g09880
rosa_chinensis RchiOBHm_Chr2g0116351
rosa_laevigata RLG00000018261
rosa_multiflora Rmu_sc0015281.1_g000003
rosa_roxburghii Rroxscaffold_2G00127300
rosa_rugosa Rorug02G0205800
rosa_samantha Rh2AG261300 Rh2BG272700 Rh2CG267700 Rh2DG269500
rosa_wichuraiana Rw2G020490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 485, 560
Acc65I GGTACC 1 cut(s) 303
AccB1I GGYRCC 2 cut(s) 85, 303
AccBSI CCGCTC 2 cut(s) 596, 670
AccII CGCG 1 cut(s) 168
AciI CCGC 9 cut(s) 89, 166, 240, 259, 288, 527, 596, 655, 670
AclI AACGTT 1 cut(s) 698
AclWI GGATC 2 cut(s) 171, 434
AcuI CTGAAG 1 cut(s) 157
AcyI GRCGYC 1 cut(s) 86
AfaI GTAC 3 cut(s) 78, 305, 650
AfiI CCNNNNNNNGG 1 cut(s) 186
AgsI TTSAA 1 cut(s) 559
AhdI GACNNNNNGTC 1 cut(s) 43
AluBI AGCT 8 cut(s) 247, 342, 360, 373, 411, 472, 571, 739
AluI AGCT 8 cut(s) 247, 342, 360, 373, 411, 472, 571, 739
Alw21I GWGCWC 2 cut(s) 413, 741
AlwI GGATC 2 cut(s) 171, 434
Ama87I CYCGRG 3 cut(s) 115, 434, 608
AoxI GGCC 5 cut(s) 313, 540, 628, 657, 731
ApaI GGGCCC 2 cut(s) 632, 661
ApeKI GCWGC 2 cut(s) 394, 571
ArsI GACNNNNNNTTYG 2 cut(s) 144, 176
Asp718I GGTACC 1 cut(s) 303
AspLEI GCGC 1 cut(s) 88
AspS9I GGNCC 8 cut(s) 37, 189, 313, 628, 629, 657, 658, 731
AsuC2I CCSGG 6 cut(s) 237, 317, 609, 610, 632, 716
AsuHPI GGTGA 3 cut(s) 331, 433, 464
AsuII TTCGAA 1 cut(s) 683
AsuNHI GCTAGC 1 cut(s) 373
AvaI CYCGRG 3 cut(s) 115, 434, 608
AvaII GGWCC 2 cut(s) 37, 189
BaeGI GKGCMC 2 cut(s) 632, 661
BaeI ACNNNNGTAYC 2 cut(s) 60, 93
BanI GGYRCC 2 cut(s) 85, 303
BanII GRGCYC 5 cut(s) 413, 632, 661, 716, 741
BbsI GAAGAC 3 cut(s) 154, 163, 521
Bbv12I GWGCWC 2 cut(s) 413, 741
BbvI GCAGC 2 cut(s) 381, 558
BccI CCATC 4 cut(s) 206, 412, 629, 637
BceAI ACGGC 1 cut(s) 211
BcgI CGANNNNNNTGC 2 cut(s) 280, 314
BcnI CCSGG 6 cut(s) 237, 317, 609, 610, 632, 716
BfaI CTAG 1 cut(s) 374
BfmI CTRYAG 1 cut(s) 395
BfoI RGCGCY 1 cut(s) 89
BglI GCCNNNNNGGC 1 cut(s) 636
BisI GCNGC 5 cut(s) 89, 241, 289, 395, 572
BlsI GCNGC 5 cut(s) 90, 242, 290, 396, 573
Bme1390I CCNGG 6 cut(s) 237, 317, 609, 610, 632, 716
Bme18I GGWCC 2 cut(s) 37, 189
BmeRI GACNNNNNGTC 1 cut(s) 43
BmeT110I CYCGRG 3 cut(s) 115, 434, 608
BmgT120I GGNCC 8 cut(s) 37, 189, 313, 628, 629, 657, 658, 731
BmiI GGNNCC 5 cut(s) 9, 87, 305, 630, 659
BmrFI CCNGG 6 cut(s) 237, 317, 609, 610, 632, 716
BmsI GCATC 1 cut(s) 59
BmtI GCTAGC 1 cut(s) 377
BpiI GAAGAC 3 cut(s) 154, 163, 521
BplI GAGNNNNNCTC 4 cut(s) 395, 427, 415, 447
BpmI CTGGAG 1 cut(s) 110
Bpu10I CCTNAGC 2 cut(s) 412, 735
Bpu14I TTCGAA 1 cut(s) 683
BpuEI CTTGAG 1 cut(s) 560
BpuMI CCSGG 6 cut(s) 237, 317, 609, 610, 632, 716
BsaHI GRCGYC 1 cut(s) 86
BsaJI CCNNGG 3 cut(s) 235, 543, 608
BsaWI WCCGGW 2 cut(s) 186, 306
BsaXI ACNNNNNCTCC 2 cut(s) 585, 615
Bsc4I CCNNNNNNNGG 1 cut(s) 186
Bse118I RCCGGY 1 cut(s) 82
BseDI CCNNGG 3 cut(s) 235, 543, 608
BseGI GGATG 4 cut(s) 50, 183, 423, 640
BseLI CCNNNNNNNGG 1 cut(s) 186
BseMII CTCAG 3 cut(s) 426, 726, 754
BseRI GAGGAG 3 cut(s) 46, 113, 563
BseSI GKGCMC 2 cut(s) 632, 661
BseXI GCAGC 2 cut(s) 381, 558
BseYI CCCAGC 2 cut(s) 104, 356
Bsh1236I CGCG 1 cut(s) 168
BshFI GGCC 5 cut(s) 315, 542, 630, 659, 733
BshNI GGYRCC 2 cut(s) 85, 303
BsiHKAI GWGCWC 2 cut(s) 413, 741
BsiHKCI CYCGRG 3 cut(s) 115, 434, 608
BsiSI CCGG 8 cut(s) 83, 187, 237, 307, 316, 609, 632, 716
BslFI GGGAC 1 cut(s) 214
BslI CCNNNNNNNGG 1 cut(s) 186
BsmFI GGGAC 1 cut(s) 214
BsnI GGCC 5 cut(s) 315, 542, 630, 659, 733
BsoBI CYCGRG 3 cut(s) 115, 434, 608
Bsp119I TTCGAA 1 cut(s) 683
Bsp120I GGGCCC 2 cut(s) 628, 657
Bsp1286I GDGCHC 5 cut(s) 413, 632, 661, 716, 741
Bsp143I GATC 2 cut(s) 176, 426
BspACI CCGC 9 cut(s) 89, 166, 240, 259, 288, 527, 596, 655, 670
BspANI GGCC 5 cut(s) 315, 542, 630, 659, 733
BspCNI CTCAG 3 cut(s) 425, 727, 753
BspFNI CGCG 1 cut(s) 168
BspLI GGNNCC 5 cut(s) 9, 87, 305, 630, 659
BspMAI CTGCAG 1 cut(s) 399
BspOI GCTAGC 1 cut(s) 377
BspPI GGATC 2 cut(s) 171, 434
BspT104I TTCGAA 1 cut(s) 683
BspT107I GGYRCC 2 cut(s) 85, 303
BsrBI CCGCTC 2 cut(s) 596, 670
BsrFI RCCGGY 1 cut(s) 82
BssAI RCCGGY 1 cut(s) 82
BssECI CCNNGG 3 cut(s) 235, 543, 608
BssMI GATC 2 cut(s) 176, 426
BssNI GRCGYC 1 cut(s) 86
Bst4CI ACNGT 2 cut(s) 41, 265
Bst6I CTCTTC 2 cut(s) 440, 668
BstACI GRCGYC 1 cut(s) 86
BstBI TTCGAA 1 cut(s) 683
BstC8I GCNNGC 6 cut(s) 217, 245, 293, 375, 598, 657
BstDEI CTNAG 4 cut(s) 345, 412, 735, 740
BstDSI CCRYGG 1 cut(s) 543
BstF5I GGATG 4 cut(s) 50, 183, 423, 640
BstFNI CGCG 1 cut(s) 168
BstH2I RGCGCY 1 cut(s) 89
BstHHI GCGC 1 cut(s) 88
BstKTI GATC 2 cut(s) 179, 429
BstMBI GATC 2 cut(s) 176, 426
BstMWI GCNNNNNNNGC 3 cut(s) 321, 379, 636
BstNSI RCATGY 2 cut(s) 219, 655
BstSCI CCNGG 6 cut(s) 235, 315, 607, 608, 630, 714
BstSFI CTRYAG 1 cut(s) 395
BstSLI GKGCMC 2 cut(s) 632, 661
BstUI CGCG 1 cut(s) 168
BstV1I GCAGC 2 cut(s) 381, 558
BstV2I GAAGAC 3 cut(s) 154, 163, 521
BstX2I RGATCY 1 cut(s) 176
BstYI RGATCY 1 cut(s) 176
BsuRI GGCC 5 cut(s) 315, 542, 630, 659, 733
BtgI CCRYGG 1 cut(s) 543
BtsCI GGATG 4 cut(s) 50, 183, 423, 640
Cac8I GCNNGC 6 cut(s) 217, 245, 293, 375, 598, 657
CfoI GCGC 1 cut(s) 88
Cfr10I RCCGGY 1 cut(s) 82
Cfr13I GGNCC 8 cut(s) 37, 189, 313, 628, 629, 657, 658, 731
Cfr9I CCCGGG 1 cut(s) 608
CseI GACGC 1 cut(s) 157
Csp6I GTAC 3 cut(s) 77, 304, 649
CviAII CATG 5 cut(s) 216, 379, 475, 565, 652
CviQI GTAC 3 cut(s) 77, 304, 649
DdeI CTNAG 4 cut(s) 345, 412, 735, 740
DinI GGCGCC 1 cut(s) 87
DpnI GATC 2 cut(s) 178, 428
DpnII GATC 2 cut(s) 176, 426
DrdI GACNNNNNNGTC 2 cut(s) 485, 560
DriI GACNNNNNGTC 1 cut(s) 43
DseDI GACNNNNNNGTC 2 cut(s) 485, 560
Eam1104I CTCTTC 2 cut(s) 440, 668
Eam1105I GACNNNNNGTC 1 cut(s) 43
EarI CTCTTC 2 cut(s) 440, 668
Ecl136II GAGCTC 2 cut(s) 411, 739
Eco24I GRGCYC 5 cut(s) 413, 632, 661, 716, 741
Eco47I GGWCC 2 cut(s) 37, 189
Eco53kI GAGCTC 2 cut(s) 411, 739
Eco57I CTGAAG 1 cut(s) 157
Eco88I CYCGRG 3 cut(s) 115, 434, 608
EcoICRI GAGCTC 2 cut(s) 411, 739
EcoO109I RGGNCCY 2 cut(s) 628, 658
EcoT38I GRGCYC 5 cut(s) 413, 632, 661, 716, 741
EgeI GGCGCC 1 cut(s) 87
EheI GGCGCC 1 cut(s) 87
FaeI CATG 5 cut(s) 219, 382, 478, 568, 655
FaqI GGGAC 1 cut(s) 214
FatI CATG 5 cut(s) 215, 378, 474, 564, 651
FauI CCCGC 3 cut(s) 589, 648, 663
Fnu4HI GCNGC 5 cut(s) 89, 241, 289, 395, 572
FokI GGATG 4 cut(s) 37, 170, 430, 647
FriOI GRGCYC 5 cut(s) 413, 632, 661, 716, 741
Fsp4HI GCNGC 5 cut(s) 89, 241, 289, 395, 572
FspBI CTAG 1 cut(s) 374
GlaI GCGC 1 cut(s) 87
GluI GCNGC 5 cut(s) 89, 241, 289, 395, 572
GsaI CCCAGC 2 cut(s) 108, 360
GsuI CTGGAG 1 cut(s) 110
HaeII RGCGCY 1 cut(s) 89
HaeIII GGCC 5 cut(s) 315, 542, 630, 659, 733
HapII CCGG 8 cut(s) 83, 187, 237, 307, 316, 609, 632, 716
HgaI GACGC 1 cut(s) 157
HhaI GCGC 1 cut(s) 88
Hin1I GRCGYC 1 cut(s) 86
Hin1II CATG 5 cut(s) 219, 382, 478, 568, 655
Hin6I GCGC 1 cut(s) 86
HinP1I GCGC 1 cut(s) 86
HinfI GANTC 4 cut(s) 251, 282, 349, 689
HpaII CCGG 8 cut(s) 83, 187, 237, 307, 316, 609, 632, 716
HphI GGTGA 3 cut(s) 331, 433, 464
Hpy188I TCNGA 4 cut(s) 176, 281, 619, 694
Hpy188III TCNNGA 2 cut(s) 434, 490
HpyAV CCTTC 4 cut(s) 304, 394, 679, 713
HpyCH4III ACNGT 2 cut(s) 41, 265
HpyCH4IV ACGT 4 cut(s) 75, 390, 621, 698
HpyCH4V TGCA 3 cut(s) 219, 382, 397
HpyF10VI GCNNNNNNNGC 3 cut(s) 321, 379, 636
HpyF3I CTNAG 4 cut(s) 345, 412, 735, 740
HpySE526I ACGT 4 cut(s) 75, 390, 621, 698
Hsp92I GRCGYC 1 cut(s) 86
Hsp92II CATG 5 cut(s) 219, 382, 478, 568, 655
HspAI GCGC 1 cut(s) 86
KasI GGCGCC 1 cut(s) 85
KpnI GGTACC 1 cut(s) 307
Kzo9I GATC 2 cut(s) 176, 426
LmnI GCTCC 3 cut(s) 7, 568, 593
Lsp1109I GCAGC 2 cut(s) 381, 558
LweI GCATC 1 cut(s) 59
MaeI CTAG 1 cut(s) 374
MaeII ACGT 4 cut(s) 75, 390, 621, 698
MaeIII GTNAC 2 cut(s) 265, 583
MalI GATC 2 cut(s) 178, 428
MbiI CCGCTC 2 cut(s) 596, 670
MboI GATC 2 cut(s) 176, 426
MboII GAAGA 6 cut(s) 154, 163, 457, 523, 526, 655
MflI RGATCY 1 cut(s) 176
MhlI GDGCHC 5 cut(s) 413, 632, 661, 716, 741
Mly113I GGCGCC 1 cut(s) 86
MlyI GAGTC 2 cut(s) 291, 343
MmeI TCCRAC 1 cut(s) 279
MseI TTAA 2 cut(s) 138, 531
MspA1I CMGCKG 1 cut(s) 288
MspI CCGG 8 cut(s) 83, 187, 237, 307, 316, 609, 632, 716
MspR9I CCNGG 6 cut(s) 237, 317, 609, 610, 632, 716
MvnI CGCG 1 cut(s) 168
MwoI GCNNNNNNNGC 3 cut(s) 321, 379, 636
NarI GGCGCC 1 cut(s) 86
NciI CCSGG 6 cut(s) 237, 317, 609, 610, 632, 716
NdeII GATC 2 cut(s) 176, 426
NheI GCTAGC 1 cut(s) 373
NlaIII CATG 5 cut(s) 219, 382, 478, 568, 655
NlaIV GGNNCC 5 cut(s) 9, 87, 305, 630, 659
NmuCI GTSAC 1 cut(s) 583
NspI RCATGY 2 cut(s) 219, 655
NspV TTCGAA 1 cut(s) 683
PaeI GCATGC 1 cut(s) 219
PaeR7I CTCGAG 2 cut(s) 115, 434
PfeI GAWTC 2 cut(s) 251, 689
PkrI GCNGC 5 cut(s) 90, 242, 290, 396, 573
PleI GAGTC 2 cut(s) 290, 343
PluTI GGCGCC 1 cut(s) 89
PpsI GAGTC 2 cut(s) 290, 343
Psp124BI GAGCTC 2 cut(s) 413, 741
Psp1406I AACGTT 1 cut(s) 698
PspFI CCCAGC 2 cut(s) 104, 356
PspN4I GGNNCC 5 cut(s) 9, 87, 305, 630, 659
PspOMI GGGCCC 2 cut(s) 628, 657
PspPI GGNCC 8 cut(s) 37, 189, 313, 628, 629, 657, 658, 731
PspXI VCTCGAGB 1 cut(s) 115
PstI CTGCAG 1 cut(s) 399
PsuI RGATCY 1 cut(s) 176
RsaI GTAC 3 cut(s) 78, 305, 650
RsaNI GTAC 3 cut(s) 77, 304, 649
SacI GAGCTC 2 cut(s) 413, 741
SaqAI TTAA 2 cut(s) 138, 531
SatI GCNGC 5 cut(s) 89, 241, 289, 395, 572
Sau3AI GATC 2 cut(s) 176, 426
Sau96I GGNCC 8 cut(s) 37, 189, 313, 628, 629, 657, 658, 731
SchI GAGTC 2 cut(s) 291, 343
ScrFI CCNGG 6 cut(s) 237, 317, 609, 610, 632, 716
SduI GDGCHC 5 cut(s) 413, 632, 661, 716, 741
SfaNI GCATC 1 cut(s) 59
SfcI CTRYAG 1 cut(s) 395
SfoI GGCGCC 1 cut(s) 87
Sfr274I CTCGAG 2 cut(s) 115, 434
SfuI TTCGAA 1 cut(s) 683
SgrAI CRCCGGYG 1 cut(s) 82
SinI GGWCC 2 cut(s) 37, 189
SlaI CTCGAG 2 cut(s) 115, 434
SmaI CCCGGG 1 cut(s) 610
SmlI CTYRAG 3 cut(s) 115, 434, 575
SmoI CTYRAG 3 cut(s) 115, 434, 575
SphI GCATGC 1 cut(s) 219
SsiI CCGC 9 cut(s) 89, 166, 240, 259, 288, 527, 596, 655, 670
SspDI GGCGCC 1 cut(s) 85
SspMI CTAG 1 cut(s) 374
SstI GAGCTC 2 cut(s) 413, 741
StyD4I CCNGG 6 cut(s) 235, 315, 607, 608, 630, 714
TaaI ACNGT 2 cut(s) 41, 265
TaiI ACGT 4 cut(s) 78, 393, 624, 701
TaqI TCGA 6 cut(s) 116, 249, 369, 435, 666, 683
TauI GCSGC 3 cut(s) 91, 243, 291
TfiI GAWTC 2 cut(s) 251, 689
Tru1I TTAA 2 cut(s) 138, 531
Tru9I TTAA 2 cut(s) 138, 531
TseFI GTSAC 1 cut(s) 583
TseI GCWGC 2 cut(s) 394, 571
Tsp45I GTSAC 1 cut(s) 583
TspDTI ATGAA 1 cut(s) 352
TspGWI ACGGA 3 cut(s) 33, 169, 560
TspMI CCCGGG 1 cut(s) 608
VpaK11BI GGWCC 2 cut(s) 37, 189
XceI RCATGY 2 cut(s) 219, 655
XhoI CTCGAG 2 cut(s) 115, 434
XmaI CCCGGG 1 cut(s) 608
XspI CTAG 1 cut(s) 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.