MD12G1109100.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0015683
Physical Location & Seq
Forward (+)
17287964 .. 17289854
1891 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1109100.v1.1.491

Sequence Viewer

Length: 831 bp
ATGGCAGACATTTACCCTTCCTACATCGAAATTAGCGACGACGACGACCAAATCCAACAGGAGCAACACCCAGAGCTGCCGATCAACGATCATCCAGAACACGAAGACCTCGACGACGACTTAGCAGTAGACCCGGATTATGAACAGTACCTTGCCGGACTACTAGCCTCTAGCTTCTCTCAAAATGCTGCCGGAATTGATTCCGGCGACGAGGATGGGTTCGGTGTTCCTCCAAGTGCTGCCGAAATTGCTGACGAAACAGATTTTGGCGACGAGACTGAGGAAGCTGATTTTTTGAATGTTGCCGCAATTGCCGGCGAAACGGAACAGTCAGGTGGGGCCGAGGAAGGCGGAGAGAAGCGGAGAAGAATAGGAGGGCCCGAAACGTCGTCGTCGGCGGCGGGTGGAATTGGCAGCAAACAGGGCTTGATTTGCTCCATTTGCTTGGATTCTAGGACCGTTGAGGGTGATCACCGCATCTGTTCTCTTCCTTGTGGGCACCTATTTGGGATGTCTTGCATAAACAGATGGCTACAGCATAAAGATACCGGGAAGTGTCCTATATGCAACCGAAAAAAATGCGAGTTGAAGGATGTTTTGAAACTTTTCGTATCATATCCATGGGATGATTCTGTTGATGATGATGAATCAGAAAAGCAAAAGACGATTCGGGAACTCAAGGATAAATGTGCTTCTCTTGAGAATGAGCTTGCTGCGTTGCGCAAGAACGAAGCTAAACTGCAGCTTGAAGATGAACAACGTACGAAGGGTATGGAAGAAAAATTCACTGATCAACTTGCTCGTAGTTTGGACACAGATAGAAATTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000278 GO:0000724 GO:0000725 GO:0002039 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006302 GO:0006310 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009628 GO:0009987 GO:0010212 GO:0010564 GO:0010948 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0022402 GO:0031297 GO:0031570 GO:0031571 GO:0031974 GO:0031981 GO:0032446 GO:0033554 GO:0034641 GO:0034645 GO:0036211 GO:0036297 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0044773 GO:0044774 GO:0044783 GO:0044819 GO:0045005 GO:0045786 GO:0045930 GO:0046483 GO:0048519 GO:0048523 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0051726 GO:0065007 GO:0070013 GO:0070647 GO:0071704 GO:0080134 GO:0080135 GO:0090304 GO:0090734 GO:0097371 GO:0140096 GO:1901360 GO:1901564 GO:1901576 GO:1901976 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902806 GO:1902807 GO:1903047 GO:2000001 GO:2000045 GO:2000134 GO:2001020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

277

Amino Acids

30.41

Weight (kDa)

4.31

Isoelectric Point (pI)

51.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 145 - 190 1.8e-10 Ring finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 722
AccB1I GGYRCC 1 cut(s) 498
AccI GTMKAC 1 cut(s) 129
AciI CCGC 6 cut(s) 306, 351, 361, 398, 401, 475
AcsI RAATTY 2 cut(s) 782, 823
AfaI GTAC 2 cut(s) 149, 763
AgsI TTSAA 4 cut(s) 298, 589, 601, 749
AjuI GAANNNNNNNTTGG 2 cut(s) 249, 281
AluBI AGCT 6 cut(s) 76, 174, 287, 709, 734, 745
AluI AGCT 6 cut(s) 76, 174, 287, 709, 734, 745
Alw26I GTCTC 1 cut(s) 269
AoxI GGCC 2 cut(s) 339, 377
ApaI GGGCCC 1 cut(s) 381
ApeKI GCWGC 6 cut(s) 76, 188, 239, 414, 713, 742
ApoI RAATTY 2 cut(s) 782, 823
Asp700I GAANNNNTTC 2 cut(s) 199, 605
AspLEI GCGC 1 cut(s) 723
AspS9I GGNCC 4 cut(s) 339, 377, 378, 456
AsuC2I CCSGG 2 cut(s) 134, 550
AsuHPI GGTGA 2 cut(s) 464, 479
AvaII GGWCC 1 cut(s) 456
BaeGI GKGCMC 2 cut(s) 381, 501
BanI GGYRCC 1 cut(s) 498
BanII GRGCYC 1 cut(s) 381
BbsI GAAGAC 1 cut(s) 111
BbvI GCAGC 6 cut(s) 63, 175, 226, 426, 700, 754
BccI CCATC 2 cut(s) 209, 522
BcgI CGANNNNNNTGC 2 cut(s) 561, 595
BclI TGATCA 2 cut(s) 469, 790
BcnI CCSGG 2 cut(s) 134, 550
BcoDI GTCTC 1 cut(s) 269
BfaI CTAG 3 cut(s) 164, 171, 453
BfmI CTRYAG 2 cut(s) 533, 740
BisI GCNGC 8 cut(s) 77, 189, 240, 306, 399, 415, 714, 743
BlsI GCNGC 8 cut(s) 78, 190, 241, 307, 400, 416, 715, 744
Bme1390I CCNGG 2 cut(s) 134, 550
Bme18I GGWCC 1 cut(s) 456
BmgT120I GGNCC 4 cut(s) 339, 377, 378, 456
BmiI GGNNCC 3 cut(s) 340, 379, 500
BmrFI CCNGG 2 cut(s) 134, 550
BmsI GCATC 1 cut(s) 486
BpiI GAAGAC 1 cut(s) 111
BpuEI CTTGAG 2 cut(s) 662, 719
BpuMI CCSGG 2 cut(s) 134, 550
BsaJI CCNNGG 2 cut(s) 342, 620
Bse118I RCCGGY 1 cut(s) 314
BseDI CCNNGG 2 cut(s) 342, 620
BseGI GGATG 5 cut(s) 91, 220, 516, 598, 631
BseMII CTCAG 1 cut(s) 270
BseSI GKGCMC 2 cut(s) 381, 501
BseXI GCAGC 6 cut(s) 63, 175, 226, 426, 700, 754
BshFI GGCC 2 cut(s) 341, 379
BshNI GGYRCC 1 cut(s) 498
BsiSI CCGG 6 cut(s) 134, 156, 192, 204, 315, 549
BsiWI CGTACG 1 cut(s) 761
BsmAI GTCTC 1 cut(s) 269
BsnI GGCC 2 cut(s) 341, 379
Bsp120I GGGCCC 1 cut(s) 377
Bsp1286I GDGCHC 2 cut(s) 381, 501
Bsp143I GATC 4 cut(s) 81, 88, 469, 790
Bsp19I CCATGG 1 cut(s) 620
BspACI CCGC 6 cut(s) 306, 351, 361, 398, 401, 475
BspANI GGCC 2 cut(s) 341, 379
BspCNI CTCAG 1 cut(s) 271
BspLI GGNNCC 3 cut(s) 340, 379, 500
BspMAI CTGCAG 1 cut(s) 744
BspT107I GGYRCC 1 cut(s) 498
BsrFI RCCGGY 1 cut(s) 314
BssAI RCCGGY 1 cut(s) 314
BssECI CCNNGG 2 cut(s) 342, 620
BssMI GATC 4 cut(s) 81, 88, 469, 790
BssT1I CCWWGG 1 cut(s) 620
Bst4CI ACNGT 3 cut(s) 147, 330, 460
Bst6I CTCTTC 1 cut(s) 492
BstC8I GCNNGC 2 cut(s) 316, 711
BstDEI CTNAG 2 cut(s) 121, 279
BstDSI CCRYGG 1 cut(s) 620
BstF5I GGATG 5 cut(s) 91, 220, 516, 598, 631
BstHHI GCGC 1 cut(s) 723
BstKTI GATC 4 cut(s) 84, 91, 472, 793
BstMAI GTCTC 1 cut(s) 269
BstMBI GATC 4 cut(s) 81, 88, 469, 790
BstMWI GCNNNNNNNGC 5 cut(s) 248, 311, 423, 432, 441
BstSCI CCNGG 2 cut(s) 132, 548
BstSFI CTRYAG 2 cut(s) 533, 740
BstSLI GKGCMC 2 cut(s) 381, 501
BstV1I GCAGC 6 cut(s) 63, 175, 226, 426, 700, 754
BstV2I GAAGAC 1 cut(s) 111
BstXI CCANNNNNNTGG 1 cut(s) 445
BsuRI GGCC 2 cut(s) 341, 379
BtgI CCRYGG 1 cut(s) 620
BtsCI GGATG 5 cut(s) 91, 220, 516, 598, 631
BtsIMutI CAGTG 1 cut(s) 786
Cac8I GCNNGC 2 cut(s) 316, 711
CfoI GCGC 1 cut(s) 723
Cfr10I RCCGGY 1 cut(s) 314
Cfr13I GGNCC 4 cut(s) 339, 377, 378, 456
Csp6I GTAC 2 cut(s) 148, 762
CviAII CATG 1 cut(s) 621
CviQI GTAC 2 cut(s) 148, 762
DdeI CTNAG 2 cut(s) 121, 279
DpnI GATC 4 cut(s) 83, 90, 471, 792
DpnII GATC 4 cut(s) 81, 88, 469, 790
Eam1104I CTCTTC 1 cut(s) 492
EarI CTCTTC 1 cut(s) 492
EciI GGCGGA 1 cut(s) 366
Eco130I CCWWGG 1 cut(s) 620
Eco24I GRGCYC 1 cut(s) 381
Eco47I GGWCC 1 cut(s) 456
EcoO109I RGGNCCY 1 cut(s) 377
EcoT14I CCWWGG 1 cut(s) 620
EcoT38I GRGCYC 1 cut(s) 381
ErhI CCWWGG 1 cut(s) 620
FaeI CATG 1 cut(s) 624
FaiI YATR 8 cut(s) 141, 521, 540, 563, 565, 616, 622, 773
FatI CATG 1 cut(s) 620
FauI CCCGC 1 cut(s) 394
FbaI TGATCA 2 cut(s) 469, 790
FblI GTMKAC 1 cut(s) 129
Fnu4HI GCNGC 8 cut(s) 77, 189, 240, 306, 399, 415, 714, 743
FokI GGATG 5 cut(s) 78, 227, 523, 605, 638
FriOI GRGCYC 1 cut(s) 381
Fsp4HI GCNGC 8 cut(s) 77, 189, 240, 306, 399, 415, 714, 743
FspBI CTAG 3 cut(s) 164, 171, 453
FspI TGCGCA 1 cut(s) 722
GlaI GCGC 1 cut(s) 722
GluI GCNGC 8 cut(s) 77, 189, 240, 306, 399, 415, 714, 743
HaeIII GGCC 2 cut(s) 341, 379
HapII CCGG 6 cut(s) 134, 156, 192, 204, 315, 549
HhaI GCGC 1 cut(s) 723
Hin1II CATG 1 cut(s) 624
Hin6I GCGC 1 cut(s) 721
HinP1I GCGC 1 cut(s) 721
HinfI GANTC 5 cut(s) 200, 449, 629, 647, 667
HpaII CCGG 6 cut(s) 134, 156, 192, 204, 315, 549
HphI GGTGA 2 cut(s) 464, 479
Hpy166II GTNNAC 1 cut(s) 130
Hpy188I TCNGA 1 cut(s) 652
Hpy188III TCNNGA 4 cut(s) 95, 671, 698, 828
Hpy8I GTNNAC 1 cut(s) 130
HpyAV CCTTC 4 cut(s) 27, 341, 583, 760
HpyCH4III ACNGT 3 cut(s) 147, 330, 460
HpyCH4IV ACGT 2 cut(s) 386, 760
HpyCH4V TGCA 3 cut(s) 519, 567, 742
HpyF10VI GCNNNNNNNGC 5 cut(s) 248, 311, 423, 432, 441
HpyF3I CTNAG 2 cut(s) 121, 279
HpySE526I ACGT 2 cut(s) 386, 760
Hsp92II CATG 1 cut(s) 624
HspAI GCGC 1 cut(s) 721
KroI GCCGGC 1 cut(s) 314
KroNI GCCGGC 1 cut(s) 316
Ksp22I TGATCA 2 cut(s) 469, 790
Kzo9I GATC 4 cut(s) 81, 88, 469, 790
LmnI GCTCC 2 cut(s) 61, 440
Lsp1109I GCAGC 6 cut(s) 63, 175, 226, 426, 700, 754
LweI GCATC 1 cut(s) 486
MaeI CTAG 3 cut(s) 164, 171, 453
MaeII ACGT 2 cut(s) 386, 760
MalI GATC 4 cut(s) 83, 90, 471, 792
MboI GATC 4 cut(s) 81, 88, 469, 790
MboII GAAGA 5 cut(s) 116, 378, 479, 761, 788
MfeI CAATTG 1 cut(s) 309
MhlI GDGCHC 2 cut(s) 381, 501
MluCI AATT 7 cut(s) 30, 195, 246, 309, 408, 782, 823
MmeI TCCRAC 1 cut(s) 79
MnlI CCTC 8 cut(s) 119, 178, 205, 240, 274, 337, 368, 457
MroNI GCCGGC 1 cut(s) 314
MroXI GAANNNNTTC 2 cut(s) 199, 605
MslI CAYNNNNRTG 1 cut(s) 619
MspI CCGG 6 cut(s) 134, 156, 192, 204, 315, 549
MspR9I CCNGG 2 cut(s) 134, 550
MunI CAATTG 1 cut(s) 309
MwoI GCNNNNNNNGC 5 cut(s) 248, 311, 423, 432, 441
NaeI GCCGGC 1 cut(s) 316
NciI CCSGG 2 cut(s) 134, 550
NcoI CCATGG 1 cut(s) 620
NdeII GATC 4 cut(s) 81, 88, 469, 790
NgoMIV GCCGGC 1 cut(s) 314
NlaIII CATG 1 cut(s) 624
NlaIV GGNNCC 3 cut(s) 340, 379, 500
NmeAIII GCCGAG 1 cut(s) 367
NsbI TGCGCA 1 cut(s) 722
PcsI WCGNNNNNNNCGW 2 cut(s) 33, 108
PdiI GCCGGC 1 cut(s) 316
PdmI GAANNNNTTC 2 cut(s) 199, 605
PfeI GAWTC 5 cut(s) 200, 449, 629, 647, 667
Pfl23II CGTACG 1 cut(s) 761
PkrI GCNGC 8 cut(s) 78, 190, 241, 307, 400, 416, 715, 744
PspLI CGTACG 1 cut(s) 761
PspN4I GGNNCC 3 cut(s) 340, 379, 500
PspOMI GGGCCC 1 cut(s) 377
PspPI GGNCC 4 cut(s) 339, 377, 378, 456
PstI CTGCAG 1 cut(s) 744
RsaI GTAC 2 cut(s) 149, 763
RsaNI GTAC 2 cut(s) 148, 762
RseI CAYNNNNRTG 1 cut(s) 619
SatI GCNGC 8 cut(s) 77, 189, 240, 306, 399, 415, 714, 743
Sau3AI GATC 4 cut(s) 81, 88, 469, 790
Sau96I GGNCC 4 cut(s) 339, 377, 378, 456
ScrFI CCNGG 2 cut(s) 134, 550
SduI GDGCHC 2 cut(s) 381, 501
SfaNI GCATC 1 cut(s) 486
SfcI CTRYAG 2 cut(s) 533, 740
SinI GGWCC 1 cut(s) 456
SmiMI CAYNNNNRTG 1 cut(s) 619
SmlI CTYRAG 2 cut(s) 677, 698
SmoI CTYRAG 2 cut(s) 677, 698
Sse9I AATT 7 cut(s) 30, 195, 246, 309, 408, 782, 823
SsiI CCGC 6 cut(s) 306, 351, 361, 398, 401, 475
SspMI CTAG 3 cut(s) 164, 171, 453
StyD4I CCNGG 2 cut(s) 132, 548
StyI CCWWGG 1 cut(s) 620
TaaI ACNGT 3 cut(s) 147, 330, 460
TaiI ACGT 2 cut(s) 389, 763
TaqI TCGA 2 cut(s) 27, 111
TasI AATT 7 cut(s) 30, 195, 246, 309, 408, 782, 823
TauI GCSGC 2 cut(s) 308, 401
TfiI GAWTC 5 cut(s) 200, 449, 629, 647, 667
TscAI CASTG 1 cut(s) 793
TseI GCWGC 6 cut(s) 76, 188, 239, 414, 713, 742
TspDTI ATGAA 3 cut(s) 156, 660, 768
TspGWI ACGGA 1 cut(s) 338
TspRI CASTG 1 cut(s) 793
VpaK11BI GGWCC 1 cut(s) 456
XapI RAATTY 2 cut(s) 782, 823
XmiI GTMKAC 1 cut(s) 129
XmnI GAANNNNTTC 2 cut(s) 199, 605
XspI CTAG 3 cut(s) 164, 171, 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.