Rroxscaffold_4G00310140

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0015683
Physical Location & Seq
Forward (+)
32047003 .. 32047527
525 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00310140.1

Sequence Viewer

Length: 525 bp
ATGATGACTTCCGAATATCCCAATAGTTGTGCCATGAGAATGCTCGCGGATGGCACAGGACAAGTTACACCACAACAAAAACCACAAGTGTATCCCCAGTGCAGCCCTCCCCACCCTCTGGATTCCTTGGGATATTCGTTGGACCAGTGCCCGATTTGCTTGCAGCCGTGTCCCTACGAAGGCAACCATGAAAGAGTCTGCCTAGTCTCTTGTGGGCATGTATACGGCCGGTCTTGCATAACTAGATGGATTGAACACAAAAAGGCCAACGCAAAGTGCCCGTCGTGCAACAAGAAATGCAAGTTGAGGGACATGAAAAGGATTTATGCATCACCCGCGGAGGATCAAAGTAGTGCACAAAGAATGAAAGAACTCGAGGCTGAGGTAGTTTCTCTGAGGGCTTCAAACGCTGCTTTAATCGATATAGGGAAGTTAAATGGCTATGTATATAATGCACAACAAAAAGAAAACAAGAAGTTGAAGGCAATGCGCATCAGGGAGACAGCAAGTTATAGTATGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000278 GO:0000724 GO:0000725 GO:0002039 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006302 GO:0006310 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009628 GO:0009987 GO:0010212 GO:0010564 GO:0010948 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0022402 GO:0031297 GO:0031570 GO:0031571 GO:0031974 GO:0031981 GO:0032446 GO:0033554 GO:0034641 GO:0034645 GO:0036211 GO:0036297 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0044773 GO:0044774 GO:0044783 GO:0044819 GO:0045005 GO:0045786 GO:0045930 GO:0046483 GO:0048519 GO:0048523 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0051726 GO:0065007 GO:0070013 GO:0070647 GO:0071704 GO:0080134 GO:0080135 GO:0090304 GO:0090734 GO:0097371 GO:0140096 GO:1901360 GO:1901564 GO:1901576 GO:1901976 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902806 GO:1902807 GO:1903047 GO:2000001 GO:2000045 GO:2000134 GO:2001020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

174

Amino Acids

19.58

Weight (kDa)

8.94

Isoelectric Point (pI)

52.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 48 - 97 1.6e-08 Ring finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 491
AccB7I CCANNNNNTGG 1 cut(s) 118
AccI GTMKAC 1 cut(s) 222
AccII CGCG 2 cut(s) 47, 338
AciI CCGC 3 cut(s) 47, 336, 338
AclWI GGATC 1 cut(s) 351
AcoI YGGCCR 1 cut(s) 226
AfiI CCNNNNNNNGG 2 cut(s) 118, 179
AgsI TTSAA 3 cut(s) 254, 405, 481
Alw21I GWGCWC 1 cut(s) 358
Alw26I GTCTC 2 cut(s) 211, 494
Alw44I GTGCAC 1 cut(s) 354
AlwI GGATC 1 cut(s) 351
Ama87I CYCGRG 1 cut(s) 374
AoxI GGCC 2 cut(s) 226, 264
ApaLI GTGCAC 1 cut(s) 354
ApeKI GCWGC 3 cut(s) 102, 163, 410
ArsI GACNNNNNNTTYG 2 cut(s) 266, 298
AspLEI GCGC 1 cut(s) 492
AspS9I GGNCC 1 cut(s) 142
AsuHPI GGTGA 1 cut(s) 324
AvaI CYCGRG 1 cut(s) 374
AvaII GGWCC 1 cut(s) 142
BaeGI GKGCMC 3 cut(s) 152, 281, 358
BaeI ACNNNNGTAYC 2 cut(s) 74, 107
Bbv12I GWGCWC 1 cut(s) 358
BbvCI CCTCAGC 1 cut(s) 381
BbvI GCAGC 3 cut(s) 114, 175, 397
BccI CCATC 2 cut(s) 44, 240
BceAI ACGGC 2 cut(s) 151, 241
BcgI CGANNNNNNTGC 2 cut(s) 142, 176
BciVI GTATCC 1 cut(s) 102
BcoDI GTCTC 2 cut(s) 211, 494
BfaI CTAG 2 cut(s) 203, 243
BfuI GTATCC 1 cut(s) 102
BisI GCNGC 3 cut(s) 103, 164, 411
BlsI GCNGC 3 cut(s) 104, 165, 412
Bme18I GGWCC 1 cut(s) 142
BmeT110I CYCGRG 1 cut(s) 374
BmgT120I GGNCC 1 cut(s) 142
BmrI ACTGGG 1 cut(s) 91
BmsI GCATC 2 cut(s) 338, 501
BmuI ACTGGG 1 cut(s) 91
Bpu10I CCTNAGC 1 cut(s) 381
Bsa29I ATCGAT 1 cut(s) 420
BsaJI CCNNGG 2 cut(s) 126, 336
Bsc4I CCNNNNNNNGG 2 cut(s) 118, 179
Bse118I RCCGGY 1 cut(s) 228
Bse1I ACTGG 2 cut(s) 97, 145
Bse3DI GCAATG 1 cut(s) 492
BseCI ATCGAT 1 cut(s) 420
BseDI CCNNGG 2 cut(s) 126, 336
BseGI GGATG 1 cut(s) 55
BseLI CCNNNNNNNGG 2 cut(s) 118, 179
BseMI GCAATG 1 cut(s) 492
BseMII CTCAG 2 cut(s) 372, 386
BseNI ACTGG 2 cut(s) 97, 145
BseSI GKGCMC 3 cut(s) 152, 281, 358
BseX3I CGGCCG 1 cut(s) 226
BseXI GCAGC 3 cut(s) 114, 175, 397
BsgI GTGCAG 1 cut(s) 121
Bsh1236I CGCG 2 cut(s) 47, 338
Bsh1285I CGRYCG 1 cut(s) 229
BshFI GGCC 2 cut(s) 228, 266
BshVI ATCGAT 1 cut(s) 420
BsiEI CGRYCG 1 cut(s) 229
BsiHKAI GWGCWC 1 cut(s) 358
BsiHKCI CYCGRG 1 cut(s) 374
BsiSI CCGG 1 cut(s) 229
BslFI GGGAC 2 cut(s) 156, 323
BslI CCNNNNNNNGG 2 cut(s) 118, 179
BsmAI GTCTC 2 cut(s) 211, 494
BsmFI GGGAC 2 cut(s) 156, 323
BsmI GAATGC 1 cut(s) 45
BsnI GGCC 2 cut(s) 228, 266
BsoBI CYCGRG 1 cut(s) 374
Bsp1286I GDGCHC 3 cut(s) 152, 281, 358
Bsp143I GATC 1 cut(s) 343
BspACI CCGC 3 cut(s) 47, 336, 338
BspANI GGCC 2 cut(s) 228, 266
BspCNI CTCAG 2 cut(s) 373, 387
BspDI ATCGAT 1 cut(s) 420
BspFNI CGCG 2 cut(s) 47, 338
BspPI GGATC 1 cut(s) 351
BsrDI GCAATG 1 cut(s) 492
BsrFI RCCGGY 1 cut(s) 228
BsrI ACTGG 2 cut(s) 97, 145
BssAI RCCGGY 1 cut(s) 228
BssECI CCNNGG 2 cut(s) 126, 336
BssMI GATC 1 cut(s) 343
BssNAI GTATAC 1 cut(s) 223
BssT1I CCWWGG 1 cut(s) 126
Bst1107I GTATAC 1 cut(s) 223
BstC8I GCNNGC 2 cut(s) 45, 161
BstDEI CTNAG 2 cut(s) 381, 395
BstDSI CCRYGG 1 cut(s) 336
BstF5I GGATG 1 cut(s) 55
BstFNI CGCG 2 cut(s) 47, 338
BstHHI GCGC 1 cut(s) 492
BstKTI GATC 1 cut(s) 346
BstMAI GTCTC 2 cut(s) 211, 494
BstMBI GATC 1 cut(s) 343
BstMCI CGRYCG 1 cut(s) 229
BstMWI GCNNNNNNNGC 5 cut(s) 156, 234, 285, 335, 407
BstNSI RCATGY 1 cut(s) 221
BstSLI GKGCMC 3 cut(s) 152, 281, 358
BstUI CGCG 2 cut(s) 47, 338
BstV1I GCAGC 3 cut(s) 114, 175, 397
BstZ17I GTATAC 1 cut(s) 223
BstZI CGGCCG 1 cut(s) 226
Bsu15I ATCGAT 1 cut(s) 420
BsuI GTATCC 1 cut(s) 102
BsuRI GGCC 2 cut(s) 228, 266
BsuTUI ATCGAT 1 cut(s) 420
BtgI CCRYGG 1 cut(s) 336
BtsCI GGATG 1 cut(s) 55
BtsIMutI CAGTG 2 cut(s) 104, 152
Cac8I GCNNGC 2 cut(s) 45, 161
CfoI GCGC 1 cut(s) 492
Cfr10I RCCGGY 1 cut(s) 228
Cfr13I GGNCC 1 cut(s) 142
Cfr42I CCGCGG 1 cut(s) 339
ClaI ATCGAT 1 cut(s) 420
CviAII CATG 4 cut(s) 34, 188, 218, 313
CviJI RGCY 7 cut(s) 105, 166, 228, 266, 380, 401, 441
CviKI_1 RGCY 7 cut(s) 105, 166, 228, 266, 380, 401, 441
DdeI CTNAG 2 cut(s) 381, 395
DpnI GATC 1 cut(s) 345
DpnII GATC 1 cut(s) 343
EaeI YGGCCR 1 cut(s) 226
EagI CGGCCG 1 cut(s) 226
EclXI CGGCCG 1 cut(s) 226
Eco130I CCWWGG 1 cut(s) 126
Eco47I GGWCC 1 cut(s) 142
Eco52I CGGCCG 1 cut(s) 226
Eco88I CYCGRG 1 cut(s) 374
EcoT14I CCWWGG 1 cut(s) 126
EcoT22I ATGCAT 1 cut(s) 331
ErhI CCWWGG 1 cut(s) 126
FaeI CATG 4 cut(s) 37, 191, 221, 316
FaqI GGGAC 2 cut(s) 156, 323
FatI CATG 4 cut(s) 33, 187, 217, 312
FauI CCCGC 1 cut(s) 343
FblI GTMKAC 1 cut(s) 222
Fnu4HI GCNGC 3 cut(s) 103, 164, 411
FokI GGATG 1 cut(s) 62
Fsp4HI GCNGC 3 cut(s) 103, 164, 411
FspAI RTGCGCAY 1 cut(s) 491
FspBI CTAG 2 cut(s) 203, 243
FspI TGCGCA 1 cut(s) 491
GlaI GCGC 1 cut(s) 491
GluI GCNGC 3 cut(s) 103, 164, 411
HaeIII GGCC 2 cut(s) 228, 266
HapII CCGG 1 cut(s) 229
HhaI GCGC 1 cut(s) 492
Hin1II CATG 4 cut(s) 37, 191, 221, 316
Hin6I GCGC 1 cut(s) 490
HinP1I GCGC 1 cut(s) 490
HinfI GANTC 2 cut(s) 122, 195
HpaII CCGG 1 cut(s) 229
HphI GGTGA 1 cut(s) 324
Hpy166II GTNNAC 2 cut(s) 223, 356
Hpy188I TCNGA 2 cut(s) 13, 396
Hpy188III TCNNGA 1 cut(s) 119
Hpy8I GTNNAC 2 cut(s) 223, 356
Hpy99I CGWCG 1 cut(s) 286
HpyAV CCTTC 2 cut(s) 173, 475
HpyCH4V TGCA 8 cut(s) 102, 163, 237, 288, 300, 329, 356, 455
HpyF10VI GCNNNNNNNGC 5 cut(s) 156, 234, 285, 335, 407
HpyF3I CTNAG 2 cut(s) 381, 395
Hsp92II CATG 4 cut(s) 37, 191, 221, 316
HspAI GCGC 1 cut(s) 490
KspI CCGCGG 1 cut(s) 339
Kzo9I GATC 1 cut(s) 343
LpnPI CCDG 6 cut(s) 42, 104, 110, 158, 242, 481
Lsp1109I GCAGC 3 cut(s) 114, 175, 397
LweI GCATC 2 cut(s) 338, 501
MaeI CTAG 2 cut(s) 203, 243
MaeIII GTNAC 1 cut(s) 64
MalI GATC 1 cut(s) 345
MboI GATC 1 cut(s) 343
MhlI GDGCHC 3 cut(s) 152, 281, 358
MlyI GAGTC 1 cut(s) 204
MmeI TCCRAC 1 cut(s) 120
MnlI CCTC 7 cut(s) 117, 126, 300, 334, 370, 376, 390
Mph1103I ATGCAT 1 cut(s) 331
MseI TTAA 3 cut(s) 416, 434, 523
MslI CAYNNNNRTG 1 cut(s) 38
MspA1I CMGCKG 1 cut(s) 338
MspI CCGG 1 cut(s) 229
Mva1269I GAATGC 1 cut(s) 45
MvnI CGCG 2 cut(s) 47, 338
MwoI GCNNNNNNNGC 5 cut(s) 156, 234, 285, 335, 407
NdeII GATC 1 cut(s) 343
NlaIII CATG 4 cut(s) 37, 191, 221, 316
NsbI TGCGCA 1 cut(s) 491
NsiI ATGCAT 1 cut(s) 331
NspI RCATGY 1 cut(s) 221
PaeR7I CTCGAG 1 cut(s) 374
PctI GAATGC 1 cut(s) 45
PfeI GAWTC 1 cut(s) 122
PflMI CCANNNNNTGG 1 cut(s) 118
PkrI GCNGC 3 cut(s) 104, 165, 412
PleI GAGTC 1 cut(s) 203
PpsI GAGTC 1 cut(s) 203
PspPI GGNCC 1 cut(s) 142
PspXI VCTCGAGB 1 cut(s) 374
RseI CAYNNNNRTG 1 cut(s) 38
SacII CCGCGG 1 cut(s) 339
SaqAI TTAA 3 cut(s) 416, 434, 523
SatI GCNGC 3 cut(s) 103, 164, 411
Sau3AI GATC 1 cut(s) 343
Sau96I GGNCC 1 cut(s) 142
SchI GAGTC 1 cut(s) 204
SduI GDGCHC 3 cut(s) 152, 281, 358
SetI ASST 1 cut(s) 387
SfaNI GCATC 2 cut(s) 338, 501
Sfr274I CTCGAG 1 cut(s) 374
Sfr303I CCGCGG 1 cut(s) 339
SgrBI CCGCGG 1 cut(s) 339
SinI GGWCC 1 cut(s) 142
SlaI CTCGAG 1 cut(s) 374
SmiMI CAYNNNNRTG 1 cut(s) 38
SmlI CTYRAG 1 cut(s) 374
SmoI CTYRAG 1 cut(s) 374
SsiI CCGC 3 cut(s) 47, 336, 338
SspMI CTAG 2 cut(s) 203, 243
StyI CCWWGG 1 cut(s) 126
TaqI TCGA 2 cut(s) 375, 420
TfiI GAWTC 1 cut(s) 122
Tru1I TTAA 3 cut(s) 416, 434, 523
Tru9I TTAA 3 cut(s) 416, 434, 523
TscAI CASTG 2 cut(s) 104, 152
TseI GCWGC 3 cut(s) 102, 163, 410
TspDTI ATGAA 3 cut(s) 204, 329, 380
TspRI CASTG 2 cut(s) 104, 152
Van91I CCANNNNNTGG 1 cut(s) 118
VneI GTGCAC 1 cut(s) 354
VpaK11BI GGWCC 1 cut(s) 142
XceI RCATGY 1 cut(s) 221
XhoI CTCGAG 1 cut(s) 374
XmiI GTMKAC 1 cut(s) 222
XspI CTAG 2 cut(s) 203, 243
Zsp2I ATGCAT 1 cut(s) 331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.