Rorug01G0170800

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0015683
Physical Location & Seq
Forward (+)
23225894 .. 23232844
6951 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0170800.1

Sequence Viewer

Length: 3288 bp
ATGATTTCTCATACTTCGCACTCCTCTATCAGCCACCATGGCTACCTCACTTGTGCGGCTCAACCCCCAGCACACTCCACTCCTCCACATCTTCAACACGACGTCGTCCATGGCGGCCATCATAAGAGGACTCTTTGTGAACTTTATAATGATACTATTGTGACTTCCTCTGCAATGGAAGTTGATGCCGGAACTCCGAATTCACAAATTCCAGCTCAACACAAAAAAAGAAGGACCGGAGTTTTGTTGTCCGAGACTTTTAGTAATGGTAATGGCGCTTTTTCCTTACCCTACCTACCAACTCTTGAAGCAGCTGATTACTATGTGCAGCCTTCTTTGAAGGAACTGGCTGCTCGAGAGCGTATGGATCCTGGTTATTCTACCCGAGTTTTGGACTTTACGGTTGGGAGGTTTGGTTATGGATCTGTCAAGTATCCTGGGGAGACTGATGTTAGGTGTTTAGAATTAGATAGAATTGTCAAGTTCCGCAGGCATGAGGTGATTGTATATGAAGATGAGAGTGCCAAGCCTTTGGTTGGCGAGGGCCTTAACAAGCCTGCTGAAGTAACTTTGATGCTACAAACAAGGGTATCGTGTGTTGACCAGAGACAGAAAGAAAATATCGTGAAAATATTGAGACAAAGTGTGGAGGGACAAGGAGCTCATTTTGTTTCGTTTAATCCAGAAAATGGGGAATGGAAATTCTTTGTTAACCATTTTAGCAGATTTGGGTTGACTGAAGAAGATGAAGAAGATATGATGATGGAAGATACTGATGTAGCTGAAGATCCTCCGGAATTAAACCATGATGAGATCTCTGATGCTGATGAAGAAAACCAAATGGATCCTACAGGAATTGTGCTCTCGCATTCTCTTCCTGCTCATCTTGGGCTTGACCCCATCAAGATGCAAGAAATGAGAATGTTGATGTTTTCTGATTTGGATGAGGAAGCCGAGGATTTGAAAGAATTACCTGCACATTATAATCCATCATTTGGTAAAGAATACTCAAGATCTCCTTTAGAAAATGCTCAAAGGATGAGTTATCGATCTACTCCACCAGCTGTGCGAAAGACCCCTCTAGCATTGCTTGAGTACAAGCATGGTAGTTTTGACTCAAACTCTCCTGGAGCCATTCTAATGGCCCAGGAAAATAAAGCCTTGCCATTGAAGACACTAAAAGAAGGTTTTAAGCTTGATCTCAGTAGTGAAACACCAGTAACTAGAAAGGATTCTCGCAACATAGTTGATGCAGGTTTGTTAATGGGTAGGTCTTTTCGAGTAGGATGGGGCCCAAATGGAATTCTTGTCCATGCTGGCACACCAATAGGCAGTAATGGTTCCCAAAGGGTGCTGTCATCTGTGATTAATATAGAGAAGGTTGCTATTGACAATGTTGTCAGAGATGAAAGTACCAAGGTTAGAGCAGAACTTGTCGAAACGGCTATCACTTCTCCATTGGAGCTTCACAAGGGACTGTTTCATGAAACAAAAGAAGTTGAAATTGGATCCTTCAGTCTGAGACTTCAGAAGCTTGTCTCGAATCGGTTTATGCTATCAGATATTTGCAGGAGTTATGTAGATATTATTGAGAGGCAGCTGGAGGTTCCTGGTTTATCTTCCTCTCCTTGTTTGGTTTTGACACATCAAATTATGATTTGGGAATTGATAAAAGTTCTTTTCTCTGAAAGGGAAAATGGCGAGAAAATGGAATCTTTTGGTGCTGATGATGAGGAAGAAATGGTGCAGGATGAGAAGCTCCCTTCACAGGAAACCGACCCGGAAGCATTACCTCTAATTAGGAGGGCCGAATTCAGTTACTGGTTACAAGAGAATGTGCGCCATCGAGTAGAAGAAATCATCTCCTTGAATGAGTCCAATTATCTGGAATCCATATTATTACTTCTGAGTGGGCGGCAGCTGGATGAAGCTGTGGAACTGGCTGCTTCTCAGGGAGATGTGAGATTGGCTTGTTTGTTAAGTCAGTCTGGTGGGTCCATAGTTAATCGTTCTGACATTGCACGACAGCTTGAGCTTTGGAGGATCAATGGACTGGATTTCAGTTTCATTGAGAAAGATAGGATAAGGCTTTATGAGTTGCTTGCGGGTAATGTCCACAGTGCTTTTCATGATATTGAAGTTGATTGGAAGAGGTTTCTTGGGTTATTGATGTGGTATCACTTAGCACCTAATACTCCATTACCCACTGTTTTCCGCACATATCATGATCTTCTTAATGAGAATAAGGCTCCATATCCTGTTCCTGTTTACATTGATGGACGTGTAGAAGTGACTGTGGATCGGAGTGCAGAGAAACGTTATGACCTATCATATTACCTTATGATGCTACATGGCAATGAAGATAGTGAAGTTGATTTCCTGAAGACCATGTTCAGTGCTTTCTCATCAACATATGATCCACTTGATTACCATATGATCTGGCATCAGCGTGCTGTGTTGGAAGCAGTTGGGGTCATCAGCGCCAATGATCTTCATGTTCTTGACATGGGATTTGTTTCCCAGTTGTTGTGTCTTGGGCAATGTCATTGGGCCATCTATGTGGTCCTTCACATGGCCCATTGTGAAGACTTTCCATATCTTCATGCTAATCTTATTAGGGAAATTTTGTTCCAATATTGTGATTCTTGGAGCTCACAAATATCACAACGCCAATTCATTGAGGACTTGGGTATTCCTAAAGCATGGCTTCATGAGGCTATGGCAGTTTATTTCAATTATTATGGGGATCTGTCAAAGGCACTTGAACACTTCATGGAATGTGCAAACTGGCAGAAAGCGCATTCTATTTTCGTAACTTCTGTTGCTCACACATTATTCTTGTCAGCCAAACACTCAGAGATATGGAGGCTAGCGACTTCCATGGAGGACCACAAATCAGAAATTGAAAATTGGGACTTGGGAGCTGGCATCTACATTTCATTCTATCTAACAAGAAGTTCATTGCAGGGAGCTGACGATGCCATGAGTGAACTGGGTTCTGTTGAAAGCAAAAATGCTGCTTCCAGAAAGTTTCTTGGTCAGTTAAACTTGTCTTTGGCTGTTTGGGGTGCCAAGCTACCAATGGATGTGAGAGTCGTCTATTCGAAGATGGCGGATGAAATATGCAACTTGATTTTGTCTGATATTAGCGAGGGCCCAACACGAGATGTTCAGTTAAGTTGCTTTGACACCGTTTTCAGTGCGCCCATACCTCAGGACCTTCGCTCAAATCATTTGCAAGATGCGGTGTCTCTCTTTACATGCTTTCTATCCGAAGTTGCTACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000278 GO:0000724 GO:0000725 GO:0002039 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006302 GO:0006310 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009628 GO:0009987 GO:0010212 GO:0010564 GO:0010948 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0022402 GO:0031297 GO:0031570 GO:0031571 GO:0031974 GO:0031981 GO:0032446 GO:0033554 GO:0034641 GO:0034645 GO:0036211 GO:0036297 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0044773 GO:0044774 GO:0044783 GO:0044819 GO:0045005 GO:0045786 GO:0045930 GO:0046483 GO:0048519 GO:0048523 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0051726 GO:0065007 GO:0070013 GO:0070647 GO:0071704 GO:0080134 GO:0080135 GO:0090304 GO:0090734 GO:0097371 GO:0140096 GO:1901360 GO:1901564 GO:1901576 GO:1901976 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902806 GO:1902807 GO:1903047 GO:2000001 GO:2000045 GO:2000134 GO:2001020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

1095

Amino Acids

123.85

Weight (kDa)

5.12

Isoelectric Point (pI)

44.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nucleoporin2 PF04096 105 - 245 2.1e-38 Nup98-96 autopeptidase S59
Nup96 PF12110 630 - 910 9.6e-77 Nuclear protein 96
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 147, 984
AasI GACNNNNNNGTC 1 cut(s) 1397
AatII GACGTC 1 cut(s) 105
Acc36I ACCTGC 2 cut(s) 982, 1244
AccB1I GGYRCC 1 cut(s) 3068
AccB7I CCANNNNNTGG 2 cut(s) 689, 995
AccIII TCCGGA 1 cut(s) 793
AciI CCGC 8 cut(s) 56, 114, 487, 1915, 2105, 2215, 3113, 3245
AclI AACGTT 1 cut(s) 2317
AcoI YGGCCR 1 cut(s) 115
AcsI RAATTY 6 cut(s) 199, 207, 701, 1302, 1811, 2622
AcuI CTGAAG 6 cut(s) 582, 759, 804, 1498, 1511, 2402
AcyI GRCGYC 1 cut(s) 102
AfaI GTAC 2 cut(s) 1097, 1414
AfiI CCNNNNNNNGG 6 cut(s) 391, 536, 689, 995, 1768, 2572
AflIII ACRYGT 1 cut(s) 2281
AjiI CACGTC 1 cut(s) 2282
AjnI CCWGG 5 cut(s) 370, 436, 1126, 1146, 1609
AjuI GAANNNNNNNTTGG 2 cut(s) 1488, 1520
AloI GAACNNNNNNTCC 2 cut(s) 2612, 2644
Alw21I GWGCWC 3 cut(s) 664, 864, 2654
Alw26I GTCTC 7 cut(s) 248, 437, 601, 631, 1516, 1543, 3255
AlwNI CAGNNNCTG 1 cut(s) 1821
Ama87I CYCGRG 2 cut(s) 354, 384
Aor13HI TCCGGA 1 cut(s) 793
AoxI GGCC 8 cut(s) 115, 544, 1143, 1291, 1806, 2550, 2574, 3154
ApaI GGGCCC 2 cut(s) 1295, 3158
ApeKI GCWGC 7 cut(s) 311, 328, 350, 1597, 1918, 1943, 3017
ApoI RAATTY 6 cut(s) 199, 207, 701, 1302, 1811, 2622
ArsI GACNNNNNNTTYG 4 cut(s) 226, 258, 2495, 2527
AseI ATTAAT 1 cut(s) 1368
Asp700I GAANNNNTTC 2 cut(s) 1231, 2589
AspLEI GCGC 5 cut(s) 278, 1842, 2483, 2800, 3205
AsuC2I CCSGG 1 cut(s) 1781
AsuHPI GGTGA 1 cut(s) 511
AsuII TTCGAA 1 cut(s) 3104
AsuNHI GCTAGC 1 cut(s) 2869
AvaI CYCGRG 2 cut(s) 354, 384
AvaII GGWCC 5 cut(s) 234, 1995, 2563, 2887, 3217
AxyI CCTNAGG 1 cut(s) 3213
BaeGI GKGCMC 2 cut(s) 1295, 3158
BaeI ACNNNNGTAYC 6 cut(s) 144, 177, 573, 606, 762, 795
BamHI GGATCC 3 cut(s) 367, 844, 1508
BanI GGYRCC 1 cut(s) 3068
BanII GRGCYC 4 cut(s) 664, 1295, 2654, 3158
BauI CACGAG 1 cut(s) 3162
BbsI GAAGAC 3 cut(s) 1178, 2390, 2592
Bbv12I GWGCWC 3 cut(s) 664, 864, 2654
BbvI GCAGC 7 cut(s) 323, 337, 340, 1609, 1930, 1930, 3004
BccI CCATC 9 cut(s) 126, 757, 908, 997, 1281, 1851, 2270, 2561, 3103
BceAI ACGGC 1 cut(s) 1458
BciT130I CCWGG 5 cut(s) 372, 438, 1128, 1148, 1611
BciVI GTATCC 1 cut(s) 444
BcnI CCSGG 1 cut(s) 1781
BcoDI GTCTC 7 cut(s) 248, 437, 601, 631, 1516, 1543, 3255
BfaI CTAG 3 cut(s) 1082, 1224, 2870
BfmI CTRYAG 1 cut(s) 849
BfoI RGCGCY 2 cut(s) 279, 2484
BfuAI ACCTGC 2 cut(s) 982, 1244
BfuI GTATCC 1 cut(s) 444
BglI GCCNNNNNGGC 1 cut(s) 39
BglII AGATCT 2 cut(s) 813, 1013
Bme1390I CCNGG 6 cut(s) 372, 438, 1128, 1148, 1611, 1781
Bme18I GGWCC 5 cut(s) 234, 1995, 2563, 2887, 3217
BmeT110I CYCGRG 2 cut(s) 354, 384
BmgBI CACGTC 1 cut(s) 2282
BmrFI CCNGG 6 cut(s) 372, 438, 1128, 1148, 1611, 1781
BmrI ACTGGG 2 cut(s) 2515, 3002
BmtI GCTAGC 1 cut(s) 2873
BmuI ACTGGG 2 cut(s) 2515, 3002
BpiI GAAGAC 3 cut(s) 1178, 2390, 2592
BpmI CTGGAG 2 cut(s) 1149, 1622
Bpu14I TTCGAA 1 cut(s) 3104
BpuEI CTTGAG 3 cut(s) 994, 1112, 2051
BpuMI CCSGG 1 cut(s) 1781
Bsa29I ATCGAT 1 cut(s) 1048
BsaHI GRCGYC 1 cut(s) 102
BsaJI CCNNGG 7 cut(s) 37, 109, 437, 954, 1146, 1416, 2880
BsaWI WCCGGW 2 cut(s) 236, 793
BsaXI ACNNNNNCTCC 2 cut(s) 400, 430
Bsc4I CCNNNNNNNGG 6 cut(s) 391, 536, 689, 995, 1768, 2572
Bse1I ACTGG 8 cut(s) 351, 1217, 1826, 1944, 2058, 2521, 2792, 2997
Bse21I CCTNAGG 1 cut(s) 3213
Bse3DI GCAATG 6 cut(s) 180, 1085, 2016, 2362, 2546, 2960
BseAI TCCGGA 1 cut(s) 793
BseBI CCWGG 5 cut(s) 372, 438, 1128, 1148, 1611
BseCI ATCGAT 1 cut(s) 1048
BseDI CCNNGG 7 cut(s) 37, 109, 437, 954, 1146, 1416, 2880
BseGI GGATG 7 cut(s) 949, 1044, 1292, 1756, 1930, 3091, 3121
BseLI CCNNNNNNNGG 6 cut(s) 391, 536, 689, 995, 1768, 2572
BseMI GCAATG 6 cut(s) 180, 1085, 2016, 2362, 2546, 2960
BseMII CTCAG 6 cut(s) 1216, 1511, 1898, 1964, 2868, 3227
BseNI ACTGG 8 cut(s) 351, 1217, 1826, 1944, 2058, 2521, 2792, 2997
BseRI GAGGAG 2 cut(s) 13, 72
BseSI GKGCMC 2 cut(s) 1295, 3158
BseXI GCAGC 7 cut(s) 323, 337, 340, 1609, 1930, 1930, 3004
BseYI CCCAGC 1 cut(s) 67
BsgI GTGCAG 4 cut(s) 347, 960, 1766, 2328
BshFI GGCC 8 cut(s) 117, 546, 1145, 1293, 1808, 2552, 2576, 3156
BshNI GGYRCC 1 cut(s) 3068
BshVI ATCGAT 1 cut(s) 1048
BsiHKAI GWGCWC 3 cut(s) 664, 864, 2654
BsiHKCI CYCGRG 2 cut(s) 354, 384
BsiSI CCGG 4 cut(s) 189, 237, 794, 1781
BslFI GGGAC 3 cut(s) 666, 1488, 2927
BslI CCNNNNNNNGG 6 cut(s) 391, 536, 689, 995, 1768, 2572
BsmAI GTCTC 7 cut(s) 248, 437, 601, 631, 1516, 1543, 3255
BsmFI GGGAC 3 cut(s) 666, 1488, 2927
BsmI GAATGC 2 cut(s) 868, 2800
BsnI GGCC 8 cut(s) 117, 546, 1145, 1293, 1808, 2552, 2576, 3156
BsoBI CYCGRG 2 cut(s) 354, 384
Bsp119I TTCGAA 1 cut(s) 3104
Bsp120I GGGCCC 2 cut(s) 1291, 3154
Bsp1286I GDGCHC 5 cut(s) 664, 864, 1295, 2654, 3158
Bsp13I TCCGGA 1 cut(s) 793
Bsp19I CCATGG 3 cut(s) 37, 109, 2880
BspACI CCGC 8 cut(s) 56, 114, 487, 1915, 2105, 2215, 3113, 3245
BspANI GGCC 8 cut(s) 117, 546, 1145, 1293, 1808, 2552, 2576, 3156
BspCNI CTCAG 6 cut(s) 1215, 1512, 1899, 1963, 2867, 3226
BspDI ATCGAT 1 cut(s) 1048
BspEI TCCGGA 1 cut(s) 793
BspHI TCATGA 4 cut(s) 1483, 2128, 2224, 2710
BspMI ACCTGC 2 cut(s) 982, 1244
BspOI GCTAGC 1 cut(s) 2873
BspT104I TTCGAA 1 cut(s) 3104
BspT107I GGYRCC 1 cut(s) 3068
BsrDI GCAATG 6 cut(s) 180, 1085, 2016, 2362, 2546, 2960
BsrI ACTGG 8 cut(s) 351, 1217, 1826, 1944, 2058, 2521, 2792, 2997
BssECI CCNNGG 7 cut(s) 37, 109, 437, 954, 1146, 1416, 2880
BssNI GRCGYC 1 cut(s) 102
BssSI CACGAG 1 cut(s) 3162
BssT1I CCWWGG 4 cut(s) 37, 109, 1416, 2880
Bst2BI CACGAG 1 cut(s) 3162
Bst2UI CCWGG 5 cut(s) 372, 438, 1128, 1148, 1611
Bst4CI ACNGT 6 cut(s) 403, 1479, 2120, 2209, 2296, 3193
Bst6I CTCTTC 2 cut(s) 879, 2144
BstACI GRCGYC 1 cut(s) 102
BstBI TTCGAA 1 cut(s) 3104
BstC8I GCNNGC 7 cut(s) 491, 558, 1318, 2103, 2451, 2871, 2926
BstDEI CTNAG 7 cut(s) 1202, 1520, 1907, 1950, 2182, 2854, 3213
BstDSI CCRYGG 3 cut(s) 37, 109, 2880
BstF5I GGATG 7 cut(s) 949, 1044, 1292, 1756, 1930, 3091, 3121
BstH2I RGCGCY 2 cut(s) 279, 2484
BstHHI GCGC 5 cut(s) 278, 1842, 2483, 2800, 3205
BstMAI GTCTC 7 cut(s) 248, 437, 601, 631, 1516, 1543, 3255
BstMWI GCNNNNNNNGC 3 cut(s) 39, 2797, 2978
BstNI CCWGG 5 cut(s) 372, 438, 1128, 1148, 1611
BstNSI RCATGY 1 cut(s) 3264
BstSCI CCNGG 6 cut(s) 370, 436, 1126, 1146, 1609, 1779
BstSFI CTRYAG 1 cut(s) 849
BstSLI GKGCMC 2 cut(s) 1295, 3158
BstV1I GCAGC 7 cut(s) 323, 337, 340, 1609, 1930, 1930, 3004
BstV2I GAAGAC 3 cut(s) 1178, 2390, 2592
BstX2I RGATCY 8 cut(s) 367, 422, 787, 813, 844, 1013, 1508, 2746
BstXI CCANNNNNNTGG 4 cut(s) 532, 1141, 1885, 2560
BstYI RGATCY 8 cut(s) 367, 422, 787, 813, 844, 1013, 1508, 2746
Bsu15I ATCGAT 1 cut(s) 1048
Bsu36I CCTNAGG 1 cut(s) 3213
BsuI GTATCC 1 cut(s) 444
BsuRI GGCC 8 cut(s) 117, 546, 1145, 1293, 1808, 2552, 2576, 3156
BsuTUI ATCGAT 1 cut(s) 1048
BtgI CCRYGG 3 cut(s) 37, 109, 2880
BtrI CACGTC 1 cut(s) 2282
BtsCI GGATG 7 cut(s) 949, 1044, 1292, 1756, 1930, 3091, 3121
BtsIMutI CAGTG 4 cut(s) 2125, 2205, 2401, 3205
BveI ACCTGC 2 cut(s) 982, 1244
Cac8I GCNNGC 7 cut(s) 491, 558, 1318, 2103, 2451, 2871, 2926
CaiI CAGNNNCTG 1 cut(s) 1821
CciI TCATGA 4 cut(s) 1483, 2128, 2224, 2710
CfoI GCGC 5 cut(s) 278, 1842, 2483, 2800, 3205
ClaI ATCGAT 1 cut(s) 1048
Csp6I GTAC 2 cut(s) 1096, 1413
CviQI GTAC 2 cut(s) 1096, 1413
DdeI CTNAG 7 cut(s) 1202, 1520, 1907, 1950, 2182, 2854, 3213
DrdI GACNNNNNNGTC 1 cut(s) 1397
DseDI GACNNNNNNGTC 1 cut(s) 1397
EaeI YGGCCR 1 cut(s) 115
Eam1104I CTCTTC 2 cut(s) 879, 2144
EarI CTCTTC 2 cut(s) 879, 2144
EciI GGCGGA 1 cut(s) 3128
Ecl136II GAGCTC 2 cut(s) 662, 2652
Eco130I CCWWGG 4 cut(s) 37, 109, 1416, 2880
Eco24I GRGCYC 4 cut(s) 664, 1295, 2654, 3158
Eco47I GGWCC 5 cut(s) 234, 1995, 2563, 2887, 3217
Eco53kI GAGCTC 2 cut(s) 662, 2652
Eco57I CTGAAG 6 cut(s) 582, 759, 804, 1498, 1511, 2402
Eco81I CCTNAGG 1 cut(s) 3213
Eco88I CYCGRG 2 cut(s) 354, 384
EcoICRI GAGCTC 2 cut(s) 662, 2652
EcoO109I RGGNCCY 4 cut(s) 544, 1291, 3154, 3217
EcoRI GAATTC 3 cut(s) 199, 1302, 1811
EcoRII CCWGG 5 cut(s) 370, 436, 1126, 1146, 1609
EcoT14I CCWWGG 4 cut(s) 37, 109, 1416, 2880
EcoT38I GRGCYC 4 cut(s) 664, 1295, 2654, 3158
ErhI CCWWGG 4 cut(s) 37, 109, 1416, 2880
FalI AAGNNNNNCTT 4 cut(s) 1003, 1035, 2691, 2723
FaqI GGGAC 3 cut(s) 666, 1488, 2927
FauI CCCGC 1 cut(s) 2098
FauNDI CATATG 2 cut(s) 2413, 2433
FokI GGATG 7 cut(s) 956, 1051, 1299, 1763, 1937, 3098, 3128
FriOI GRGCYC 4 cut(s) 664, 1295, 2654, 3158
FspBI CTAG 3 cut(s) 1082, 1224, 2870
GlaI GCGC 5 cut(s) 277, 1841, 2482, 2799, 3204
GsaI CCCAGC 1 cut(s) 71
GsuI CTGGAG 2 cut(s) 1149, 1622
HaeII RGCGCY 2 cut(s) 279, 2484
HaeIII GGCC 8 cut(s) 117, 546, 1145, 1293, 1808, 2552, 2576, 3156
HapII CCGG 4 cut(s) 189, 237, 794, 1781
HhaI GCGC 5 cut(s) 278, 1842, 2483, 2800, 3205
Hin1I GRCGYC 1 cut(s) 102
Hin6I GCGC 5 cut(s) 276, 1840, 2481, 2798, 3203
HinP1I GCGC 5 cut(s) 276, 1840, 2481, 2798, 3203
HincII GTYRAC 3 cut(s) 601, 712, 735
HindII GTYRAC 3 cut(s) 601, 712, 735
HindIII AAGCTT 2 cut(s) 1193, 1532
HinfI GANTC 9 cut(s) 130, 1115, 1232, 1543, 1712, 1874, 1889, 2642, 3093
HpaI GTTAAC 1 cut(s) 712
HpaII CCGG 4 cut(s) 189, 237, 794, 1781
HphI GGTGA 1 cut(s) 511
Hpy166II GTNNAC 7 cut(s) 140, 601, 712, 735, 2116, 2269, 2990
Hpy8I GTNNAC 7 cut(s) 140, 601, 712, 735, 2116, 2269, 2990
Hpy99I CGWCG 2 cut(s) 104, 107
HpyAV CCTTC 9 cut(s) 225, 334, 342, 1178, 1372, 1522, 1773, 2576, 3230
HpyCH4III ACNGT 6 cut(s) 403, 1479, 2120, 2209, 2296, 3193
HpyCH4IV ACGT 3 cut(s) 102, 2281, 2317
HpyF10VI GCNNNNNNNGC 3 cut(s) 39, 2797, 2978
HpyF3I CTNAG 7 cut(s) 1202, 1520, 1907, 1950, 2182, 2854, 3213
HpySE526I ACGT 3 cut(s) 102, 2281, 2317
Hsp92I GRCGYC 1 cut(s) 102
HspAI GCGC 5 cut(s) 276, 1840, 2481, 2798, 3203
Kpn2I TCCGGA 1 cut(s) 793
KspAI GTTAAC 1 cut(s) 712
LmnI GCTCC 8 cut(s) 659, 1130, 1462, 1764, 2254, 2649, 2921, 2969
Lsp1109I GCAGC 7 cut(s) 323, 337, 340, 1609, 1930, 1930, 3004
MaeI CTAG 3 cut(s) 1082, 1224, 2870
MaeII ACGT 3 cut(s) 102, 2281, 2317
MaeIII GTNAC 7 cut(s) 160, 565, 1219, 1817, 1824, 2290, 2812
MflI RGATCY 8 cut(s) 367, 422, 787, 813, 844, 1013, 1508, 2746
MhlI GDGCHC 5 cut(s) 664, 864, 1295, 2654, 3158
MlyI GAGTC 4 cut(s) 124, 1109, 1883, 3102
MmeI TCCRAC 1 cut(s) 2439
MroI TCCGGA 1 cut(s) 793
MroXI GAANNNNTTC 2 cut(s) 1231, 2589
MslI CAYNNNNRTG 5 cut(s) 905, 1139, 2355, 2448, 2558
MspA1I CMGCKG 4 cut(s) 314, 1064, 1600, 1921
MspI CCGG 4 cut(s) 189, 237, 794, 1781
MspR9I CCNGG 6 cut(s) 372, 438, 1128, 1148, 1611, 1781
Mva1269I GAATGC 2 cut(s) 868, 2800
MvaI CCWGG 5 cut(s) 372, 438, 1128, 1148, 1611
MwoI GCNNNNNNNGC 3 cut(s) 39, 2797, 2978
NciI CCSGG 1 cut(s) 1781
NcoI CCATGG 3 cut(s) 37, 109, 2880
NdeI CATATG 2 cut(s) 2413, 2433
NheI GCTAGC 1 cut(s) 2869
NmeAIII GCCGAG 1 cut(s) 979
NmuCI GTSAC 2 cut(s) 160, 2290
NspI RCATGY 1 cut(s) 3264
NspV TTCGAA 1 cut(s) 3104
PaeR7I CTCGAG 1 cut(s) 354
PagI TCATGA 4 cut(s) 1483, 2128, 2224, 2710
PctI GAATGC 2 cut(s) 868, 2800
PdmI GAANNNNTTC 2 cut(s) 1231, 2589
PfeI GAWTC 5 cut(s) 1232, 1543, 1712, 1889, 2642
PflFI GACNNNGTC 1 cut(s) 104
PflMI CCANNNNNTGG 2 cut(s) 689, 995
PfoI TCCNGGA 1 cut(s) 1126
PleI GAGTC 4 cut(s) 124, 1109, 1882, 3101
PpsI GAGTC 4 cut(s) 124, 1109, 1882, 3101
PpuMI RGGWCCY 1 cut(s) 3217
PshBI ATTAAT 1 cut(s) 1368
PsiI TTATAA 2 cut(s) 147, 984
Psp124BI GAGCTC 2 cut(s) 664, 2654
Psp1406I AACGTT 1 cut(s) 2317
Psp5II RGGWCCY 1 cut(s) 3217
Psp6I CCWGG 5 cut(s) 370, 436, 1126, 1146, 1609
PspFI CCCAGC 1 cut(s) 67
PspGI CCWGG 5 cut(s) 370, 436, 1126, 1146, 1609
PspOMI GGGCCC 2 cut(s) 1291, 3154
PspPPI RGGWCCY 1 cut(s) 3217
PstNI CAGNNNCTG 1 cut(s) 1821
PsuI RGATCY 8 cut(s) 367, 422, 787, 813, 844, 1013, 1508, 2746
PsyI GACNNNGTC 1 cut(s) 104
PvuII CAGCTG 4 cut(s) 314, 1064, 1600, 1921
RsaI GTAC 2 cut(s) 1097, 1414
RsaNI GTAC 2 cut(s) 1096, 1413
RseI CAYNNNNRTG 5 cut(s) 905, 1139, 2355, 2448, 2558
SacI GAGCTC 2 cut(s) 664, 2654
SchI GAGTC 4 cut(s) 124, 1109, 1883, 3102
ScrFI CCNGG 6 cut(s) 372, 438, 1128, 1148, 1611, 1781
SduI GDGCHC 5 cut(s) 664, 864, 1295, 2654, 3158
SfcI CTRYAG 1 cut(s) 849
Sfr274I CTCGAG 1 cut(s) 354
SfuI TTCGAA 1 cut(s) 3104
SinI GGWCC 5 cut(s) 234, 1995, 2563, 2887, 3217
SlaI CTCGAG 1 cut(s) 354
SmiMI CAYNNNNRTG 5 cut(s) 905, 1139, 2355, 2448, 2558
SmlI CTYRAG 4 cut(s) 354, 1009, 1091, 2030
SmoI CTYRAG 4 cut(s) 354, 1009, 1091, 2030
SsiI CCGC 8 cut(s) 56, 114, 487, 1915, 2105, 2215, 3113, 3245
SspI AATATT 2 cut(s) 633, 2636
SspMI CTAG 3 cut(s) 1082, 1224, 2870
SstI GAGCTC 2 cut(s) 664, 2654
StyD4I CCNGG 6 cut(s) 370, 436, 1126, 1146, 1609, 1779
StyI CCWWGG 4 cut(s) 37, 109, 1416, 2880
TaaI ACNGT 6 cut(s) 403, 1479, 2120, 2209, 2296, 3193
TaiI ACGT 3 cut(s) 105, 2284, 2320
TaqI TCGA 7 cut(s) 355, 1048, 1279, 1437, 1541, 1846, 3104
TatI WGTACW 1 cut(s) 1095
TauI GCSGC 3 cut(s) 59, 117, 1918
TfiI GAWTC 5 cut(s) 1232, 1543, 1712, 1889, 2642
TscAI CASTG 4 cut(s) 2125, 2212, 2401, 3205
TseFI GTSAC 2 cut(s) 160, 2290
TseI GCWGC 7 cut(s) 311, 328, 350, 1597, 1918, 1943, 3017
Tsp45I GTSAC 2 cut(s) 160, 2290
TspRI CASTG 4 cut(s) 2125, 2212, 2401, 3205
Tth111I GACNNNGTC 1 cut(s) 104
Van91I CCANNNNNTGG 2 cut(s) 689, 995
VpaK11BI GGWCC 5 cut(s) 234, 1995, 2563, 2887, 3217
VspI ATTAAT 1 cut(s) 1368
XapI RAATTY 6 cut(s) 199, 207, 701, 1302, 1811, 2622
XceI RCATGY 1 cut(s) 3264
XcmI CCANNNNNNNNNTGG 2 cut(s) 2989, 3079
XhoI CTCGAG 1 cut(s) 354
XmnI GAANNNNTTC 2 cut(s) 1231, 2589
XspI CTAG 3 cut(s) 1082, 1224, 2870
ZraI GACGTC 1 cut(s) 103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.