Prupe.7G127600_v2.0.a1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0015683
Physical Location & Seq
Forward (+)
14846058 .. 14849063
3006 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G127600.3

Sequence Viewer

Length: 741 bp
ATGCAAAACAATGGCGATCGAGACAACGAAAATCACTATGATGAAGACGAAGAAGTAGAAGAATTCGAAGAAGAACAAGAGGAAGTAGAAGAAGAAGACGAGGAGGAAGACTTCATTGATGCGGATTATGCTGCGTACCTTTCAGAACTATTACCCTCTAGTTTTGGTCACCACGCTGGCGGGATTTCTGGTGGAACAGAATCGAGAGATGGGGCAGAGAAAGGTGGAGAGAAGCGGAGAAGAATCAGAGGACCGGGAACGTTGTCATTGGGTGGAATTGGAAGCGCAGAAGGCTCCCAAGGCAATGAATGGAATCGGAGTGGAATCGATGGCCTATTTTGCCCTATCTGCTTGGACGCTTGGACCAATGACGGCGATCATCGCATCTGCTGTCTTCCCTGCGGCCACATATACGGCATGTCTTGCATAACAAAATGGCTACAGCGTAGAAATTCGAGAAAGTGTCCTCAATGCAACCAGAAATGCAAGTTGAAGGATGTTAGGAAACTTTTCGTATCACAGGTTCTTTCTGTTGATGAAGAATCACAAAAGAGGATTCGGGTACTTGAGGATAAGTGTGCTTCTCTTGAGGAGAAGGTTGCTGATCTTTCTAAGAAAGAAGCTGAATGGCTAAAGCGAGAGGGTGAACTGCAGCAAAAAGTTCAACAGTGTACAGAGCCATCGATGGACTCTGCTAAGATTATGCAGTCAATCACAGCTCAGTGCAATGACATACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000278 GO:0000724 GO:0000725 GO:0002039 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006302 GO:0006310 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009628 GO:0009987 GO:0010212 GO:0010564 GO:0010948 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0022402 GO:0031297 GO:0031570 GO:0031571 GO:0031974 GO:0031981 GO:0032446 GO:0033554 GO:0034641 GO:0034645 GO:0036211 GO:0036297 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0044773 GO:0044774 GO:0044783 GO:0044819 GO:0045005 GO:0045786 GO:0045930 GO:0046483 GO:0048519 GO:0048523 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0051726 GO:0065007 GO:0070013 GO:0070647 GO:0071704 GO:0080134 GO:0080135 GO:0090304 GO:0090734 GO:0097371 GO:0140096 GO:1901360 GO:1901564 GO:1901576 GO:1901976 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902806 GO:1902807 GO:1903047 GO:2000001 GO:2000045 GO:2000134 GO:2001020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

247

Amino Acids

27.73

Weight (kDa)

4.67

Isoelectric Point (pI)

65.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 122, 180, 235, 402
AclI AACGTT 1 cut(s) 260
AcoI YGGCCR 1 cut(s) 403
AcsI RAATTY 2 cut(s) 62, 451
AfaI GTAC 3 cut(s) 137, 564, 673
AgsI TTSAA 2 cut(s) 493, 665
AluBI AGCT 2 cut(s) 623, 719
AluI AGCT 2 cut(s) 623, 719
Alw26I GTCTC 1 cut(s) 15
AoxI GGCC 2 cut(s) 331, 403
ApeKI GCWGC 2 cut(s) 131, 652
ApoI RAATTY 2 cut(s) 62, 451
Asp700I GAANNNNTTC 1 cut(s) 509
AspLEI GCGC 1 cut(s) 287
AspS9I GGNCC 2 cut(s) 251, 363
AsuC2I CCSGG 1 cut(s) 255
AsuHPI GGTGA 2 cut(s) 161, 656
AsuII TTCGAA 1 cut(s) 66
AvaII GGWCC 2 cut(s) 251, 363
BbsI GAAGAC 4 cut(s) 51, 102, 114, 386
BbvI GCAGC 2 cut(s) 118, 664
BccI CCATC 4 cut(s) 203, 323, 679, 688
BceAI ACGGC 2 cut(s) 388, 430
BcnI CCSGG 1 cut(s) 255
BcoDI GTCTC 1 cut(s) 15
BfaI CTAG 1 cut(s) 159
BfmI CTRYAG 2 cut(s) 440, 650
BisI GCNGC 3 cut(s) 132, 403, 653
BlsI GCNGC 3 cut(s) 133, 404, 654
Bme1390I CCNGG 1 cut(s) 255
Bme18I GGWCC 2 cut(s) 251, 363
BmgT120I GGNCC 2 cut(s) 251, 363
BmiI GGNNCC 1 cut(s) 295
BmrFI CCNGG 1 cut(s) 255
BmsI GCATC 2 cut(s) 109, 393
BpiI GAAGAC 4 cut(s) 51, 102, 114, 386
Bpu14I TTCGAA 1 cut(s) 66
BpuEI CTTGAG 2 cut(s) 587, 608
BpuMI CCSGG 1 cut(s) 255
Bsa29I ATCGAT 2 cut(s) 327, 683
BsaJI CCNNGG 1 cut(s) 298
Bse3DI GCAATG 2 cut(s) 310, 733
BseCI ATCGAT 2 cut(s) 327, 683
BseDI CCNNGG 1 cut(s) 298
BseGI GGATG 1 cut(s) 502
BseMI GCAATG 2 cut(s) 310, 733
BseMII CTCAG 1 cut(s) 734
BseRI GAGGAG 2 cut(s) 116, 605
BseXI GCAGC 2 cut(s) 118, 664
Bsh1285I CGRYCG 1 cut(s) 19
BshFI GGCC 2 cut(s) 333, 405
BshVI ATCGAT 2 cut(s) 327, 683
BsiEI CGRYCG 1 cut(s) 19
BsiSI CCGG 1 cut(s) 254
BsmAI GTCTC 1 cut(s) 15
BsnI GGCC 2 cut(s) 333, 405
Bsp119I TTCGAA 1 cut(s) 66
Bsp1407I TGTACA 1 cut(s) 671
Bsp143I GATC 3 cut(s) 16, 376, 604
BspACI CCGC 4 cut(s) 122, 180, 235, 402
BspANI GGCC 2 cut(s) 333, 405
BspCNI CTCAG 1 cut(s) 733
BspDI ATCGAT 2 cut(s) 327, 683
BspLI GGNNCC 1 cut(s) 295
BspMAI CTGCAG 1 cut(s) 654
BspT104I TTCGAA 1 cut(s) 66
BsrDI GCAATG 2 cut(s) 310, 733
BsrGI TGTACA 1 cut(s) 671
BssECI CCNNGG 1 cut(s) 298
BssMI GATC 3 cut(s) 16, 376, 604
BssT1I CCWWGG 1 cut(s) 298
Bst4CI ACNGT 1 cut(s) 669
BstAPI GCANNNNNTGC 1 cut(s) 423
BstAUI TGTACA 1 cut(s) 671
BstBI TTCGAA 1 cut(s) 66
BstC8I GCNNGC 1 cut(s) 178
BstDEI CTNAG 3 cut(s) 612, 696, 720
BstEII GGTNACC 1 cut(s) 167
BstF5I GGATG 1 cut(s) 502
BstHHI GCGC 1 cut(s) 287
BstKTI GATC 3 cut(s) 19, 379, 607
BstMAI GTCTC 1 cut(s) 15
BstMBI GATC 3 cut(s) 16, 376, 604
BstMCI CGRYCG 1 cut(s) 19
BstMWI GCNNNNNNNGC 7 cut(s) 128, 291, 300, 339, 348, 381, 423
BstNSI RCATGY 1 cut(s) 421
BstPI GGTNACC 1 cut(s) 167
BstSCI CCNGG 1 cut(s) 253
BstSFI CTRYAG 2 cut(s) 440, 650
BstV1I GCAGC 2 cut(s) 118, 664
BstV2I GAAGAC 4 cut(s) 51, 102, 114, 386
Bsu15I ATCGAT 2 cut(s) 327, 683
BsuRI GGCC 2 cut(s) 333, 405
BsuTUI ATCGAT 2 cut(s) 327, 683
BtgZI GCGATG 1 cut(s) 365
BtsCI GGATG 1 cut(s) 502
BtsIMutI CAGTG 2 cut(s) 674, 728
Cac8I GCNNGC 1 cut(s) 178
CfoI GCGC 1 cut(s) 287
Cfr13I GGNCC 2 cut(s) 251, 363
ClaI ATCGAT 2 cut(s) 327, 683
CseI GACGC 1 cut(s) 365
Csp6I GTAC 3 cut(s) 136, 563, 672
CviAII CATG 1 cut(s) 418
CviJI RGCY 8 cut(s) 294, 333, 405, 439, 623, 631, 679, 719
CviKI_1 RGCY 8 cut(s) 294, 333, 405, 439, 623, 631, 679, 719
CviQI GTAC 3 cut(s) 136, 563, 672
DdeI CTNAG 3 cut(s) 612, 696, 720
DpnI GATC 3 cut(s) 18, 378, 606
DpnII GATC 3 cut(s) 16, 376, 604
EaeI YGGCCR 1 cut(s) 403
Eco130I CCWWGG 1 cut(s) 298
Eco47I GGWCC 2 cut(s) 251, 363
Eco91I GGTNACC 1 cut(s) 167
EcoO65I GGTNACC 1 cut(s) 167
EcoRI GAATTC 1 cut(s) 62
EcoT14I CCWWGG 1 cut(s) 298
ErhI CCWWGG 1 cut(s) 298
FaeI CATG 1 cut(s) 421
FaiI YATR 8 cut(s) 39, 129, 410, 412, 419, 428, 704, 734
FatI CATG 1 cut(s) 417
FauI CCCGC 1 cut(s) 173
Fnu4HI GCNGC 3 cut(s) 132, 403, 653
FokI GGATG 1 cut(s) 509
Fsp4HI GCNGC 3 cut(s) 132, 403, 653
FspBI CTAG 1 cut(s) 159
GlaI GCGC 1 cut(s) 286
GluI GCNGC 3 cut(s) 132, 403, 653
HaeIII GGCC 2 cut(s) 333, 405
HapII CCGG 1 cut(s) 254
HgaI GACGC 1 cut(s) 365
HhaI GCGC 1 cut(s) 287
Hin1II CATG 1 cut(s) 421
Hin6I GCGC 1 cut(s) 285
HinP1I GCGC 1 cut(s) 285
HinfI GANTC 7 cut(s) 200, 243, 313, 324, 542, 556, 689
HpaII CCGG 1 cut(s) 254
HphI GGTGA 2 cut(s) 161, 656
Hpy166II GTNNAC 2 cut(s) 647, 672
Hpy188I TCNGA 3 cut(s) 145, 248, 318
Hpy188III TCNNGA 4 cut(s) 20, 204, 456, 587
Hpy8I GTNNAC 2 cut(s) 647, 672
HpyAV CCTTC 3 cut(s) 284, 487, 589
HpyCH4III ACNGT 1 cut(s) 669
HpyCH4IV ACGT 1 cut(s) 260
HpyCH4V TGCA 7 cut(s) 4, 426, 474, 486, 652, 706, 726
HpyF10VI GCNNNNNNNGC 7 cut(s) 128, 291, 300, 339, 348, 381, 423
HpyF3I CTNAG 3 cut(s) 612, 696, 720
HpySE526I ACGT 1 cut(s) 260
Hsp92II CATG 1 cut(s) 421
HspAI GCGC 1 cut(s) 285
Kzo9I GATC 3 cut(s) 16, 376, 604
LmnI GCTCC 1 cut(s) 299
LpnPI CCDG 6 cut(s) 162, 174, 267, 412, 491, 506
Lsp1109I GCAGC 2 cut(s) 118, 664
LweI GCATC 2 cut(s) 109, 393
MaeI CTAG 1 cut(s) 159
MaeII ACGT 1 cut(s) 260
MaeIII GTNAC 1 cut(s) 167
MalI GATC 3 cut(s) 18, 378, 606
MboI GATC 3 cut(s) 16, 376, 604
MluCI AATT 3 cut(s) 62, 276, 451
MlyI GAGTC 1 cut(s) 683
MroXI GAANNNNTTC 1 cut(s) 509
MseI TTAA 1 cut(s) 739
MslI CAYNNNNRTG 1 cut(s) 39
MspI CCGG 1 cut(s) 254
MspR9I CCNGG 1 cut(s) 255
MwoI GCNNNNNNNGC 7 cut(s) 128, 291, 300, 339, 348, 381, 423
NciI CCSGG 1 cut(s) 255
NdeII GATC 3 cut(s) 16, 376, 604
NlaIII CATG 1 cut(s) 421
NlaIV GGNNCC 1 cut(s) 295
NmuCI GTSAC 1 cut(s) 167
NspI RCATGY 1 cut(s) 421
NspV TTCGAA 1 cut(s) 66
PdmI GAANNNNTTC 1 cut(s) 509
PfeI GAWTC 6 cut(s) 200, 243, 313, 324, 542, 556
PkrI GCNGC 3 cut(s) 133, 404, 654
Ple19I CGATCG 1 cut(s) 19
PleI GAGTC 1 cut(s) 683
PpsI GAGTC 1 cut(s) 683
Psp1406I AACGTT 1 cut(s) 260
PspEI GGTNACC 1 cut(s) 167
PspN4I GGNNCC 1 cut(s) 295
PspPI GGNCC 2 cut(s) 251, 363
PsrI GAACNNNNNNTAC 2 cut(s) 507, 539
PstI CTGCAG 1 cut(s) 654
PvuI CGATCG 1 cut(s) 19
RsaI GTAC 3 cut(s) 137, 564, 673
RsaNI GTAC 3 cut(s) 136, 563, 672
RseI CAYNNNNRTG 1 cut(s) 39
SaqAI TTAA 1 cut(s) 739
SatI GCNGC 3 cut(s) 132, 403, 653
Sau3AI GATC 3 cut(s) 16, 376, 604
Sau96I GGNCC 2 cut(s) 251, 363
SchI GAGTC 1 cut(s) 683
ScrFI CCNGG 1 cut(s) 255
SetI ASST 7 cut(s) 141, 226, 263, 525, 600, 625, 721
SfaNI GCATC 2 cut(s) 109, 393
SfcI CTRYAG 2 cut(s) 440, 650
SfuI TTCGAA 1 cut(s) 66
SinI GGWCC 2 cut(s) 251, 363
SmiMI CAYNNNNRTG 1 cut(s) 39
SmlI CTYRAG 2 cut(s) 566, 587
SmoI CTYRAG 2 cut(s) 566, 587
Sse9I AATT 3 cut(s) 62, 276, 451
SsiI CCGC 4 cut(s) 122, 180, 235, 402
SspMI CTAG 1 cut(s) 159
StyD4I CCNGG 1 cut(s) 253
StyI CCWWGG 1 cut(s) 298
TaaI ACNGT 1 cut(s) 669
TaiI ACGT 1 cut(s) 263
TaqI TCGA 6 cut(s) 19, 66, 203, 327, 455, 683
TasI AATT 3 cut(s) 62, 276, 451
TatI WGTACW 1 cut(s) 671
TauI GCSGC 1 cut(s) 405
TfiI GAWTC 6 cut(s) 200, 243, 313, 324, 542, 556
Tru1I TTAA 1 cut(s) 739
Tru9I TTAA 1 cut(s) 739
TscAI CASTG 2 cut(s) 674, 728
TseFI GTSAC 1 cut(s) 167
TseI GCWGC 2 cut(s) 131, 652
Tsp45I GTSAC 1 cut(s) 167
TspDTI ATGAA 4 cut(s) 57, 103, 321, 552
TspRI CASTG 2 cut(s) 674, 728
VpaK11BI GGWCC 2 cut(s) 251, 363
XapI RAATTY 2 cut(s) 62, 451
XceI RCATGY 1 cut(s) 421
XmnI GAANNNNTTC 1 cut(s) 509
XspI CTAG 1 cut(s) 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.