MD12G1109500.v1.1

Protein of unknown function (DUF4050)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0012852
Physical Location & Seq
Forward (+)
17373046 .. 17376405
3360 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1109500.v1.1.491

Sequence Viewer

Length: 315 bp
ATGGAAACTAATGGTAGCAATTCTAATTCCAATGAAAATCAGCCTTCCGATGCTTCAAATATTGATAGGAGAGAGAAGCTTACTGAGAAAGAAGTCTTTATTAATCATGAGGTAGCTTGGCAAGAGAGAAGAAGAGAATGGGTCGGTGATCAGTCTCAGAAATCACAAAGAGCGCCAAGAGAACCAATTATGAGCTGGACCACAACGTATGAGGATCTTCTTTTATCTACCGAACCTTTTCAGGAGCCAATACCTTTAGCTGAAATGGTGGATTTTTTAGCTAATATCTGGCAAGAAGAAGGCCTCTATGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4050

Protein Analysis

105

Amino Acids

12.23

Weight (kDa)

4.43

Isoelectric Point (pI)

62.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
clamp_Gag1-like PF13259 65 - 104 3e-10 Gag1-like, clamp domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012852)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G03440 AT5G03440 AT5G03440
fragaria_vesca FvH4_6g16540
malus_domestica MD04G1091600.v1.1 MD12G1109500.v1.1
prunus_persica Prupe.7G128200_v2.0.a1
pyrus_communis pycom04g08630
rosa_chinensis RchiOBHm_Chr3g0470451
rosa_laevigata RLG00000024266
rosa_multiflora Rmu_sc0003381.1_g000005
rosa_roxburghii Rroxscaffold_6G00410790
rosa_rugosa Rorug03G0109200
rosa_samantha Rh3AG159600 Rh3BG184100 Rh3CG174600 Rh3DG190800
rosa_wichuraiana Rw3G015100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 222
AgsI TTSAA 1 cut(s) 57
AluBI AGCT 5 cut(s) 79, 116, 195, 260, 281
AluI AGCT 5 cut(s) 79, 116, 195, 260, 281
Alw26I GTCTC 1 cut(s) 159
AlwI GGATC 1 cut(s) 222
AoxI GGCC 1 cut(s) 301
AseI ATTAAT 1 cut(s) 102
Asp700I GAANNNNTTC 1 cut(s) 237
AspLEI GCGC 1 cut(s) 175
AspS9I GGNCC 1 cut(s) 198
AsuHPI GGTGA 1 cut(s) 158
AvaII GGWCC 1 cut(s) 198
BclI TGATCA 1 cut(s) 148
BcoDI GTCTC 1 cut(s) 159
BfaI CTAG 1 cut(s) 313
BfoI RGCGCY 1 cut(s) 176
Bme18I GGWCC 1 cut(s) 198
BmgT120I GGNCC 1 cut(s) 198
BmiI GGNNCC 1 cut(s) 246
BmsI GCATC 1 cut(s) 40
BseMII CTCAG 2 cut(s) 75, 170
BshFI GGCC 1 cut(s) 303
BsmAI GTCTC 1 cut(s) 159
BsnI GGCC 1 cut(s) 303
Bsp143I GATC 2 cut(s) 148, 214
BspANI GGCC 1 cut(s) 303
BspCNI CTCAG 2 cut(s) 76, 169
BspHI TCATGA 1 cut(s) 106
BspLI GGNNCC 1 cut(s) 246
BspPI GGATC 1 cut(s) 222
BssMI GATC 2 cut(s) 148, 214
Bst6I CTCTTC 1 cut(s) 127
BstDEI CTNAG 2 cut(s) 84, 156
BstH2I RGCGCY 1 cut(s) 176
BstHHI GCGC 1 cut(s) 175
BstKTI GATC 2 cut(s) 151, 217
BstMAI GTCTC 1 cut(s) 159
BstMBI GATC 2 cut(s) 148, 214
BstX2I RGATCY 1 cut(s) 214
BstYI RGATCY 1 cut(s) 214
BsuRI GGCC 1 cut(s) 303
CciI TCATGA 1 cut(s) 106
CfoI GCGC 1 cut(s) 175
Cfr13I GGNCC 1 cut(s) 198
CviAII CATG 1 cut(s) 107
CviJI RGCY 8 cut(s) 43, 79, 116, 195, 247, 260, 281, 303
CviKI_1 RGCY 8 cut(s) 43, 79, 116, 195, 247, 260, 281, 303
DdeI CTNAG 2 cut(s) 84, 156
DpnI GATC 2 cut(s) 150, 216
DpnII GATC 2 cut(s) 148, 214
Eam1104I CTCTTC 1 cut(s) 127
EarI CTCTTC 1 cut(s) 127
Eco147I AGGCCT 1 cut(s) 303
Eco47I GGWCC 1 cut(s) 198
FaeI CATG 1 cut(s) 110
FaiI YATR 4 cut(s) 108, 191, 210, 309
FatI CATG 1 cut(s) 106
FbaI TGATCA 1 cut(s) 148
FspBI CTAG 1 cut(s) 313
GlaI GCGC 1 cut(s) 174
HaeII RGCGCY 1 cut(s) 176
HaeIII GGCC 1 cut(s) 303
HhaI GCGC 1 cut(s) 175
Hin1II CATG 1 cut(s) 110
Hin6I GCGC 1 cut(s) 173
HinP1I GCGC 1 cut(s) 173
HindIII AAGCTT 1 cut(s) 77
HphI GGTGA 1 cut(s) 158
Hpy188I TCNGA 2 cut(s) 49, 159
Hpy188III TCNNGA 2 cut(s) 107, 242
HpyAV CCTTC 2 cut(s) 54, 293
HpyCH4IV ACGT 1 cut(s) 206
HpyF3I CTNAG 2 cut(s) 84, 156
HpySE526I ACGT 1 cut(s) 206
Hsp92II CATG 1 cut(s) 110
HspAI GCGC 1 cut(s) 173
Ksp22I TGATCA 1 cut(s) 148
Kzo9I GATC 2 cut(s) 148, 214
LmnI GCTCC 1 cut(s) 244
LpnPI CCDG 3 cut(s) 181, 227, 274
LweI GCATC 1 cut(s) 40
MaeI CTAG 1 cut(s) 313
MaeII ACGT 1 cut(s) 206
MalI GATC 2 cut(s) 150, 216
MboI GATC 2 cut(s) 148, 214
MboII GAAGA 4 cut(s) 141, 144, 209, 308
MflI RGATCY 1 cut(s) 214
MluCI AATT 3 cut(s) 19, 25, 186
MnlI CCTC 3 cut(s) 103, 205, 314
MroXI GAANNNNTTC 1 cut(s) 237
MseI TTAA 1 cut(s) 102
NdeII GATC 2 cut(s) 148, 214
NlaIII CATG 1 cut(s) 110
NlaIV GGNNCC 1 cut(s) 246
PagI TCATGA 1 cut(s) 106
PceI AGGCCT 1 cut(s) 303
PdmI GAANNNNTTC 1 cut(s) 237
PshBI ATTAAT 1 cut(s) 102
PspN4I GGNNCC 1 cut(s) 246
PspPI GGNCC 1 cut(s) 198
PsuI RGATCY 1 cut(s) 214
SaqAI TTAA 1 cut(s) 102
Sau3AI GATC 2 cut(s) 148, 214
Sau96I GGNCC 1 cut(s) 198
SetI ASST 9 cut(s) 81, 114, 118, 197, 209, 238, 256, 262, 283
SfaNI GCATC 1 cut(s) 40
SgeI CNNG 8 cut(s) 119, 129, 134, 189, 208, 254, 301, 305
SinI GGWCC 1 cut(s) 198
Sse9I AATT 3 cut(s) 19, 25, 186
SseBI AGGCCT 1 cut(s) 303
SspI AATATT 1 cut(s) 61
SspMI CTAG 1 cut(s) 313
StuI AGGCCT 1 cut(s) 303
TaiI ACGT 1 cut(s) 209
TasI AATT 3 cut(s) 19, 25, 186
Tru1I TTAA 1 cut(s) 102
Tru9I TTAA 1 cut(s) 102
TspDTI ATGAA 1 cut(s) 48
VpaK11BI GGWCC 1 cut(s) 198
VspI ATTAAT 1 cut(s) 102
XcmI CCANNNNNNNNNTGG 1 cut(s) 192
XmnI GAANNNNTTC 1 cut(s) 237
XspI CTAG 1 cut(s) 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.