Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0015476

Physical Location & Seq

Genomic Coordinates Forward (+)

19133805 .. 19137778

Gene Structure 3974 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1119400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2328 bp

ATGCAAACCCAGCAAGCCCTTTCTGGGTTATCTCGGTCCGTACCTCCTCTGCTTCTCCTCCTGCTCTTATGCTCCACCTCCACATCTGGGTTCGGATCAATGGGACCAATCTCGGCCTCTTTCGGCAAAGATGGCTTCTTTTGCGCCATAGACGCTAGCGGGAAGCAGGGCGTGGTTTGTTGGGGGTTCAACAGCTCTCCATCGACGTCGTCTTCGGCGTCATCCTCGTCCTCCGCCTCTTTCAGCAACATTCCGTCGATGGCTGCTCTCTCCGGTGGTGAAAATTTTCTTTGTGGTATTTTAGCGAACACCTCGCAGGCGTATTGTTGGATCTCCGTTTCCCCAGGTATAGATCTTGTTCCTCTACCATTCAAGTCCACCGGTTACTCTCATATTGCTGCTGGGAAGAGCCATGTGTGTGCTATTAGAGGGTCGTATTACTCTGACCATGAATATGGAACTGTAGATTGCTGGGAAATTTTTGAAACTTCGAATAGAAGTTTAAGTTCGAAACAGAGCACTCTGTTTTCTGACCAGGCAATTGCTAACCTTGTCTTCAAAGGGGTTGTTTCTGGTGAAGGGTTTAGCTGTGGAGCTGTTAGAGATGGAGGGCTGATTTGTTGGGGGCCAAATTCTTCGAATTTAGGTGTTTCGGGTCCGGTACTACAGAATTTCACAGCTTTAGCTTCAGGGAGGGCCTCAGTCTGTGGAATTTCAGATTTTGGTGAGTTGAATTGTTGGGGTGATGCTGATTTGTTGGATGGTCATCCAAATGGAACTCAGTATGCGTCTTTGGCTGCTGGTGATCATCATTATTGCGGGATTCGACTAGATAATCACAGAGTGGAGTGTTGGGGGATGTTTAACTCCTCTTTGATTCCAAAGGCTTCCGGATTTATGGCGATTGCCTCGTCGGATTATACCACTTGTGGGATCAGGGAAGATGATTTGGTGATTGATTGTTGGGCTGCCAATGCAACTTCCTCCCCAACCGATTATGATCCTCCATTGCAATTGTGCAGTCCAGGTCTTTGCACTCCTGGTTCTTGTGGGCAAGGTGAGTTTGCTTTCAATGCTAGCGCACTCAATGAGGTTGATTTGACAAGCTTGTGTGTTCGAAAAGATCTTACGATTTGTTCCTCGTGCGGGTCAAATTGCTCCGAAGGATTCTTCTTGTCTAGTTCATGTACTCAACATGCTGATAGAGTGTGCACAGCTTGCTCTCTTTGCCAGAACAGCTCTTGTTGGGATATTTGTGGGCTTCAAACACCTCCAGAAATGAGGAAAAAGCTCTGGAATCATGTGCGTAGGTTGGTGATCATATTCGGGTCTTCTGCTTTGGGTTTTTTGTTGATATTAATTTGTTGGTGCCTTCTTCCCCGTTTAACTTCTCGAAAAAAAGAAGGGAGCAAAAAGCAGTTTAAATCTTGCATTGGGAAAGCAGAGTTGGAAGCTGACAATAATGAGGATCCAAACCCTCCTCCATCCGTGATTCCCTGTCCTGGGATTGCTCAAGTGTTCCGTCTCTCAGAACTAAAAGACGCCACCAACGGATTCAAGGAGTTTAACGAGCTTGGCAGGGGAAGTTTTGGCTTTGTTTACAAAGCTGTGCTAGCAGATGGACAGCAAGTTGCAGTCAAGAGAGCAAATGCTGCCACTATAATTCACACAAACAGTCGAGACTTTGAAATGGAATTAGAGGTCCTTAGCAAAATCCGCCACTGTAACATTGTAAACTTATTGGGTTACTGCTCAGAGATGGGGGAAAGGCTGCTTGTATACGAGTATATGGCCCACGGGACGCTTTATGATCATCTCCATGGTGGGCTTTCAGCCCTGAATTGGAGCCTTCGGTTGAAAATTGCAATGCAGGCTGCTAAGGGGCTTGAGTACCTTCACAAGGAATTCATGCCTCCCATTGTTCATCGCAATGTCAAGACCTCGAACATTCTTTTAGACGCTGAATGGAGTGCGCGGATTGCAGATTTCGGACTCCTTACATCTAATGACAGGGATCTCAATGGAGATTTAGCAAGTGATGTTTACAACTTTGGAGTAGTACTGCTAGAGATTCTCAGTGGAAGGAAAGCTTATGACAGAGATTACACTCCGCCAAGTGTAGTCGAATGGGCACTGCCTTTAATCAAACAGAACAAGGCAGCTGCCATAATCGACCGTTATATAGCTCTCCCGAGAAATGTTGAGCCTTTGCTCAAGCTTGCTGATATAGCAGGACTGGCGATAAAGGAAAATCCAACCGAGCGTCCTACAATGTCGGATATTGCATCTTGGTTGGAGCACATTGTGAAAGATGGATTGACCTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0042802 GO:0042803 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0046983 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Pkinase_Tyr RCC1_2

Protein Analysis

776

Amino Acids

83.54

Weight (kDa)

5.69

Isoelectric Point (pI)

36.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	520 - 667	5.8e-30	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	523 - 669	2.5e-32	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015476)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G09780
fragaria_vesca	FvH4_6g15770
malus_domestica	MD04G1098700.v1.1 MD12G1119400.v1.1
prunus_persica	Prupe.6G237300_v2.0.a1
pyrus_communis	pycom12g11840
rosa_chinensis	RchiOBHm_Chr3g0469481
rosa_laevigata	RLG00000024347
rosa_multiflora	Rmu_sc0004871.1_g000013
rosa_roxburghii	Rroxscaffold_6G00411820
rosa_rugosa	Rorug03G0102200
rosa_samantha	Rh3AG151100 Rh3BG174700 Rh3CG165800 Rh3DG200600
rosa_wichuraiana	Rw3G014270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	209
AccB1I	GGYRCC	1 cut(s)	1368
AccI	GTMKAC	1 cut(s)	1779
AccII	CGCG	1 cut(s)	1975
AccIII	TCCGGA	1 cut(s)	890
AciI	CCGC	7 cut(s)	159, 234, 819, 1146, 1717, 1975, 2111
AclWI	GGATC	7 cut(s)	103, 338, 941, 995, 1463, 1476, 2022
AcsI	RAATTY	7 cut(s)	283, 477, 631, 640, 670, 711, 1904
AcuI	CTGAAG	1 cut(s)	672
AcyI	GRCGYC	3 cut(s)	206, 218, 1542
AdeI	CACNNNGTG	2 cut(s)	844, 2305
AfaI	GTAC	5 cut(s)	42, 663, 1189, 1892, 2061
AfiI	CCNNNNNNNGG	8 cut(s)	24, 87, 930, 1146, 1502, 1503, 1842, 1900
AgeI	ACCGGT	1 cut(s)	380
AjnI	CCWGG	5 cut(s)	343, 534, 1024, 1039, 1501
AjuI	GAANNNNNNNTTGG	2 cut(s)	1430, 1462
Alw21I	GWGCWC	3 cut(s)	521, 1214, 2301
Alw26I	GTCTC	2 cut(s)	1529, 1674
Alw44I	GTGCAC	1 cut(s)	1210
AlwI	GGATC	7 cut(s)	103, 338, 941, 995, 1463, 1476, 2022
Ama87I	CYCGRG	1 cut(s)	2191
Aor13HI	TCCGGA	1 cut(s)	890
AoxI	GGCC	4 cut(s)	114, 626, 696, 1791
ApaLI	GTGCAC	1 cut(s)	1210
ApeKI	GCWGC	9 cut(s)	263, 398, 797, 968, 1652, 1771, 1874, 2159, 2162
ApoI	RAATTY	7 cut(s)	283, 477, 631, 640, 670, 711, 1904
ArsI	GACNNNNNNTTYG	2 cut(s)	196, 228
AseI	ATTAAT	1 cut(s)	1358
AsiGI	ACCGGT	1 cut(s)	380
Asp700I	GAANNNNTTC	1 cut(s)	285
AspLEI	GCGC	3 cut(s)	146, 1082, 1975
AspS9I	GGNCC	7 cut(s)	36, 104, 626, 656, 696, 1702, 1792
AsuHPI	GGTGA	8 cut(s)	290, 587, 737, 755, 815, 964, 1070, 1327
AsuII	TTCGAA	4 cut(s)	491, 509, 638, 1117
AsuNHI	GCTAGC	3 cut(s)	155, 1076, 1612
AvaI	CYCGRG	1 cut(s)	2191
AvaII	GGWCC	4 cut(s)	36, 104, 656, 1702
BaeGI	GKGCMC	2 cut(s)	1214, 2134
BamHI	GGATCC	1 cut(s)	1468
BanI	GGYRCC	1 cut(s)	1368
BauI	CACGAG	1 cut(s)	1141
BbsI	GAAGAC	3 cut(s)	204, 547, 1323
Bbv12I	GWGCWC	3 cut(s)	521, 1214, 2301
BbvI	GCAGC	9 cut(s)	250, 385, 784, 955, 1639, 1758, 1861, 2149, 2171
BccI	CCATC	9 cut(s)	125, 208, 253, 599, 755, 1492, 1613, 1753, 2306
BciT130I	CCWGG	5 cut(s)	345, 536, 1026, 1041, 1503
BclI	TGATCA	3 cut(s)	805, 1317, 1810
BcoDI	GTCTC	2 cut(s)	1529, 1674
BfaI	CTAG	6 cut(s)	156, 830, 1077, 1179, 1613, 2066
BfmI	CTRYAG	2 cut(s)	462, 665
BglII	AGATCT	2 cut(s)	352, 1123
BisI	GCNGC	9 cut(s)	264, 399, 798, 969, 1653, 1772, 1875, 2160, 2163
BlsI	GCNGC	9 cut(s)	265, 400, 799, 970, 1654, 1773, 1876, 2161, 2164
BmcAI	AGTACT	1 cut(s)	2061
Bme1390I	CCNGG	5 cut(s)	345, 536, 1026, 1041, 1503
Bme18I	GGWCC	4 cut(s)	36, 104, 656, 1702
BmeT110I	CYCGRG	1 cut(s)	2191
BmgT120I	GGNCC	7 cut(s)	36, 104, 626, 656, 696, 1702, 1792
BmiI	GGNNCC	6 cut(s)	105, 627, 657, 1370, 1470, 1847
BmrFI	CCNGG	5 cut(s)	345, 536, 1026, 1041, 1503
BmsI	GCATC	2 cut(s)	736, 2294
BmtI	GCTAGC	3 cut(s)	159, 1080, 1616
BpiI	GAAGAC	3 cut(s)	204, 547, 1323
BpmI	CTGGAG	1 cut(s)	1257
Bpu10I	CCTNAGC	2 cut(s)	1706, 1878
Bpu14I	TTCGAA	4 cut(s)	491, 509, 638, 1117
BpuEI	CTTGAG	3 cut(s)	1497, 1907, 2198
BsaBI	GATNNNNATC	2 cut(s)	765, 999
BsaHI	GRCGYC	3 cut(s)	206, 218, 1542
BsaJI	CCNNGG	4 cut(s)	343, 1502, 1795, 1819
BsaWI	WCCGGW	4 cut(s)	272, 380, 658, 890
Bsc4I	CCNNNNNNNGG	8 cut(s)	24, 87, 930, 1146, 1502, 1503, 1842, 1900
Bse118I	RCCGGY	1 cut(s)	380
Bse1I	ACTGG	1 cut(s)	2241
Bse3DI	GCAATG	3 cut(s)	1007, 1872, 1936
Bse8I	GATNNNNATC	2 cut(s)	765, 999
BseAI	TCCGGA	1 cut(s)	890
BseBI	CCWGG	5 cut(s)	345, 536, 1026, 1041, 1503
BseDI	CCNNGG	4 cut(s)	343, 1502, 1795, 1819
BseGI	GGATG	5 cut(s)	221, 766, 766, 864, 1484
BseJI	GATNNNNATC	2 cut(s)	765, 999
BseLI	CCNNNNNNNGG	8 cut(s)	24, 87, 930, 1146, 1502, 1503, 1842, 1900
BseMI	GCAATG	3 cut(s)	1007, 1872, 1936
BseMII	CTCAG	5 cut(s)	714, 794, 1542, 1767, 2089
BseNI	ACTGG	1 cut(s)	2241
BseRI	GAGGAG	4 cut(s)	36, 47, 859, 1470
BseSI	GKGCMC	2 cut(s)	1214, 2134
BseXI	GCAGC	9 cut(s)	250, 385, 784, 955, 1639, 1758, 1861, 2149, 2171
BseYI	CCCAGC	3 cut(s)	9, 401, 471
BsgI	GTGCAG	1 cut(s)	1039
Bsh1236I	CGCG	1 cut(s)	1975
Bsh1285I	CGRYCG	1 cut(s)	2176
BshFI	GGCC	4 cut(s)	116, 628, 698, 1793
BshNI	GGYRCC	1 cut(s)	1368
BshTI	ACCGGT	1 cut(s)	380
BsiEI	CGRYCG	1 cut(s)	2176
BsiHKAI	GWGCWC	3 cut(s)	521, 1214, 2301
BsiHKCI	CYCGRG	1 cut(s)	2191
BsiSI	CCGG	4 cut(s)	273, 381, 659, 891
BslFI	GGGAC	2 cut(s)	117, 1813
BslI	CCNNNNNNNGG	8 cut(s)	24, 87, 930, 1146, 1502, 1503, 1842, 1900
BsmAI	GTCTC	2 cut(s)	1529, 1674
BsmBI	CGTCTC	1 cut(s)	1529
BsmFI	GGGAC	2 cut(s)	117, 1813
BsnI	GGCC	4 cut(s)	116, 628, 698, 1793
BsoBI	CYCGRG	1 cut(s)	2191
Bsp119I	TTCGAA	4 cut(s)	491, 509, 638, 1117
Bsp1286I	GDGCHC	4 cut(s)	521, 1214, 2134, 2301
Bsp13I	TCCGGA	1 cut(s)	890
Bsp19I	CCATGG	1 cut(s)	1819
BspACI	CCGC	7 cut(s)	159, 234, 819, 1146, 1717, 1975, 2111
BspANI	GGCC	4 cut(s)	116, 628, 698, 1793
BspCNI	CTCAG	5 cut(s)	713, 793, 1541, 1766, 2088
BspEI	TCCGGA	1 cut(s)	890
BspFNI	CGCG	1 cut(s)	1975
BspLI	GGNNCC	6 cut(s)	105, 627, 657, 1370, 1470, 1847
BspOI	GCTAGC	3 cut(s)	159, 1080, 1616
BspPI	GGATC	7 cut(s)	103, 338, 941, 995, 1463, 1476, 2022
BspQI	GCTCTTC	1 cut(s)	401
BspT104I	TTCGAA	4 cut(s)	491, 509, 638, 1117
BspT107I	GGYRCC	1 cut(s)	1368
BsrDI	GCAATG	3 cut(s)	1007, 1872, 1936
BsrFI	RCCGGY	1 cut(s)	380
BsrI	ACTGG	1 cut(s)	2241
BssAI	RCCGGY	1 cut(s)	380
BssECI	CCNNGG	4 cut(s)	343, 1502, 1795, 1819
BssNAI	GTATAC	1 cut(s)	1780
BssNI	GRCGYC	3 cut(s)	206, 218, 1542
BssSI	CACGAG	1 cut(s)	1141
BssT1I	CCWWGG	1 cut(s)	1819
Bst1107I	GTATAC	1 cut(s)	1780
Bst2BI	CACGAG	1 cut(s)	1141
Bst2UI	CCWGG	5 cut(s)	345, 536, 1026, 1041, 1503
Bst4CI	ACNGT	4 cut(s)	463, 1676, 1724, 2177
Bst6I	CTCTTC	1 cut(s)	401
BstACI	GRCGYC	3 cut(s)	206, 218, 1542
BstAPI	GCANNNNNTGC	2 cut(s)	1218, 1652
BstBI	TTCGAA	4 cut(s)	491, 509, 638, 1117
BstC8I	GCNNGC	8 cut(s)	15, 157, 318, 1078, 1219, 1614, 1872, 2220
BstDEI	CTNAG	7 cut(s)	700, 780, 1528, 1706, 1753, 1878, 2075
BstDSI	CCRYGG	2 cut(s)	1795, 1819
BstENI	CCTNNNNNAGG	1 cut(s)	1898
BstF5I	GGATG	5 cut(s)	221, 766, 766, 864, 1484
BstFNI	CGCG	1 cut(s)	1975
BstHHI	GCGC	3 cut(s)	146, 1082, 1975
BstMAI	GTCTC	2 cut(s)	1529, 1674
BstMCI	CGRYCG	1 cut(s)	2176
BstNI	CCWGG	5 cut(s)	345, 536, 1026, 1041, 1503
BstNSI	RCATGY	1 cut(s)	1199
BstSCI	CCNGG	5 cut(s)	343, 534, 1024, 1039, 1501
BstSFI	CTRYAG	2 cut(s)	462, 665
BstSLI	GKGCMC	2 cut(s)	1214, 2134
BstUI	CGCG	1 cut(s)	1975
BstV1I	GCAGC	9 cut(s)	250, 385, 784, 955, 1639, 1758, 1861, 2149, 2171
BstV2I	GAAGAC	3 cut(s)	204, 547, 1323
BstX2I	RGATCY	5 cut(s)	330, 352, 1123, 1468, 2014
BstXI	CCANNNNNNTGG	1 cut(s)	455
BstYI	RGATCY	5 cut(s)	330, 352, 1123, 1468, 2014
BstZ17I	GTATAC	1 cut(s)	1780
BsuRI	GGCC	4 cut(s)	116, 628, 698, 1793
BtgI	CCRYGG	2 cut(s)	1795, 1819
BtgZI	GCGATG	1 cut(s)	1910
BtsCI	GGATG	5 cut(s)	221, 766, 766, 864, 1484
BtsI	GCAGTG	1 cut(s)	2132
BtsIMutI	CAGTG	3 cut(s)	1720, 2083, 2132
Cac8I	GCNNGC	8 cut(s)	15, 157, 318, 1078, 1219, 1614, 1872, 2220
CfoI	GCGC	3 cut(s)	146, 1082, 1975
Cfr10I	RCCGGY	1 cut(s)	380
Cfr13I	GGNCC	7 cut(s)	36, 104, 626, 656, 696, 1702, 1792
CpoI	CGGWCCG	1 cut(s)	36
CseI	GACGC	7 cut(s)	161, 207, 777, 1550, 1810, 1967, 2252
Csp6I	GTAC	5 cut(s)	41, 662, 1188, 1891, 2060
CspAI	ACCGGT	1 cut(s)	380
CspI	CGGWCCG	1 cut(s)	36
CviAII	CATG	7 cut(s)	413, 449, 1185, 1196, 1301, 1820, 1909
CviQI	GTAC	5 cut(s)	41, 662, 1188, 1891, 2060
DdeI	CTNAG	7 cut(s)	700, 780, 1528, 1706, 1753, 1878, 2075
DraI	TTTAAA	1 cut(s)	1423
DraIII	CACNNNGTG	2 cut(s)	844, 2305
Eam1104I	CTCTTC	1 cut(s)	401
EarI	CTCTTC	1 cut(s)	401
EciI	GGCGGA	3 cut(s)	223, 1706, 2100
Eco130I	CCWWGG	1 cut(s)	1819
Eco47I	GGWCC	4 cut(s)	36, 104, 656, 1702
Eco57I	CTGAAG	1 cut(s)	672
Eco88I	CYCGRG	1 cut(s)	2191
EcoNI	CCTNNNNNAGG	1 cut(s)	1898
EcoO109I	RGGNCCY	2 cut(s)	696, 1702
EcoRI	GAATTC	1 cut(s)	1904
EcoRII	CCWGG	5 cut(s)	343, 534, 1024, 1039, 1501
EcoT14I	CCWWGG	1 cut(s)	1819
ErhI	CCWWGG	1 cut(s)	1819
Esp3I	CGTCTC	1 cut(s)	1529
FaeI	CATG	7 cut(s)	416, 452, 1188, 1199, 1304, 1823, 1912
FalI	AAGNNNNNCTT	2 cut(s)	2074, 2106
FaqI	GGGAC	2 cut(s)	117, 1813
FatI	CATG	7 cut(s)	412, 448, 1184, 1195, 1300, 1819, 1908
FauI	CCCGC	3 cut(s)	152, 812, 1139
FbaI	TGATCA	3 cut(s)	805, 1317, 1810
FblI	GTMKAC	1 cut(s)	1779
Fnu4HI	GCNGC	9 cut(s)	264, 399, 798, 969, 1653, 1772, 1875, 2160, 2163
FokI	GGATG	5 cut(s)	208, 753, 773, 871, 1471
Fsp4HI	GCNGC	9 cut(s)	264, 399, 798, 969, 1653, 1772, 1875, 2160, 2163
FspBI	CTAG	6 cut(s)	156, 830, 1077, 1179, 1613, 2066
GlaI	GCGC	3 cut(s)	145, 1081, 1974
GluI	GCNGC	9 cut(s)	264, 399, 798, 969, 1653, 1772, 1875, 2160, 2163
GsaI	CCCAGC	3 cut(s)	13, 405, 475
GsuI	CTGGAG	1 cut(s)	1257
HaeIII	GGCC	4 cut(s)	116, 628, 698, 1793
HapII	CCGG	4 cut(s)	273, 381, 659, 891
HgaI	GACGC	7 cut(s)	161, 207, 777, 1550, 1810, 1967, 2252
HhaI	GCGC	3 cut(s)	146, 1082, 1975
Hin1I	GRCGYC	3 cut(s)	206, 218, 1542
Hin1II	CATG	7 cut(s)	416, 452, 1188, 1199, 1304, 1823, 1912
Hin6I	GCGC	3 cut(s)	144, 1080, 1973
HinP1I	GCGC	3 cut(s)	144, 1080, 1973
HindIII	AAGCTT	3 cut(s)	1105, 2088, 2216
HinfI	GANTC	8 cut(s)	823, 877, 1167, 1297, 1492, 1554, 1992, 2071
HpaII	CCGG	4 cut(s)	273, 381, 659, 891
HphI	GGTGA	8 cut(s)	290, 587, 737, 755, 815, 964, 1070, 1327
Hpy166II	GTNNAC	6 cut(s)	378, 1212, 1600, 1735, 1780, 2044
Hpy188III	TCNNGA	8 cut(s)	891, 1274, 1294, 1392, 1639, 1679, 1936, 2191
Hpy8I	GTNNAC	6 cut(s)	378, 1212, 1600, 1735, 1780, 2044
Hpy99I	CGWCG	4 cut(s)	208, 211, 259, 916
HpyAV	CCTTC	7 cut(s)	572, 1157, 1382, 1397, 1859, 1904, 2076
HpyCH4III	ACNGT	4 cut(s)	463, 1676, 1724, 2177
HpyCH4IV	ACGT	1 cut(s)	206
HpyF3I	CTNAG	7 cut(s)	700, 780, 1528, 1706, 1753, 1878, 2075
HpySE526I	ACGT	1 cut(s)	206
Hsp92I	GRCGYC	3 cut(s)	206, 218, 1542
Hsp92II	CATG	7 cut(s)	416, 452, 1188, 1199, 1304, 1823, 1912
HspAI	GCGC	3 cut(s)	144, 1080, 1973
Kpn2I	TCCGGA	1 cut(s)	890
Ksp22I	TGATCA	3 cut(s)	805, 1317, 1810
LguI	GCTCTTC	1 cut(s)	401
LmnI	GCTCC	6 cut(s)	77, 593, 1163, 1407, 1845, 2296
Lsp1109I	GCAGC	9 cut(s)	250, 385, 784, 955, 1639, 1758, 1861, 2149, 2171
LweI	GCATC	2 cut(s)	736, 2294
MaeI	CTAG	6 cut(s)	156, 830, 1077, 1179, 1613, 2066
MaeII	ACGT	1 cut(s)	206
MaeIII	GTNAC	3 cut(s)	383, 1724, 1745
MboII	GAAGA	8 cut(s)	204, 418, 547, 627, 953, 1162, 1323, 1367
MfeI	CAATTG	2 cut(s)	540, 1013
MflI	RGATCY	5 cut(s)	330, 352, 1123, 1468, 2014
MhlI	GDGCHC	4 cut(s)	521, 1214, 2134, 2301
MlyI	GAGTC	1 cut(s)	1986
MmeI	TCCRAC	7 cut(s)	308, 738, 894, 1428, 2256, 2274, 2279
MroI	TCCGGA	1 cut(s)	890
MroXI	GAANNNNTTC	1 cut(s)	285
MseI	TTAA	7 cut(s)	503, 864, 1358, 1385, 1422, 1566, 2141
MslI	CAYNNNNRTG	5 cut(s)	417, 453, 771, 1818, 1929
MspA1I	CMGCKG	1 cut(s)	2162
MspI	CCGG	4 cut(s)	273, 381, 659, 891
MspR9I	CCNGG	5 cut(s)	345, 536, 1026, 1041, 1503
MunI	CAATTG	2 cut(s)	540, 1013
MvaI	CCWGG	5 cut(s)	345, 536, 1026, 1041, 1503
MvnI	CGCG	1 cut(s)	1975
NcoI	CCATGG	1 cut(s)	1819
NheI	GCTAGC	3 cut(s)	155, 1076, 1612
NlaIII	CATG	7 cut(s)	416, 452, 1188, 1199, 1304, 1823, 1912
NlaIV	GGNNCC	6 cut(s)	105, 627, 657, 1370, 1470, 1847
NmeAIII	GCCGAG	1 cut(s)	92
NspI	RCATGY	1 cut(s)	1199
NspV	TTCGAA	4 cut(s)	491, 509, 638, 1117
PciSI	GCTCTTC	1 cut(s)	401
PcsI	WCGNNNNNNNCGW	1 cut(s)	215
PdmI	GAANNNNTTC	1 cut(s)	285
PfeI	GAWTC	7 cut(s)	823, 877, 1167, 1297, 1492, 1554, 2071
PflFI	GACNNNGTC	1 cut(s)	208
PinAI	ACCGGT	1 cut(s)	380
PkrI	GCNGC	9 cut(s)	265, 400, 799, 970, 1654, 1773, 1876, 2161, 2164
PleI	GAGTC	1 cut(s)	1986
PpsI	GAGTC	1 cut(s)	1986
PpuMI	RGGWCCY	1 cut(s)	1702
PshBI	ATTAAT	1 cut(s)	1358
Psp5II	RGGWCCY	1 cut(s)	1702
Psp6I	CCWGG	5 cut(s)	343, 534, 1024, 1039, 1501
PspFI	CCCAGC	3 cut(s)	9, 401, 471
PspGI	CCWGG	5 cut(s)	343, 534, 1024, 1039, 1501
PspN4I	GGNNCC	6 cut(s)	105, 627, 657, 1370, 1470, 1847
PspPI	GGNCC	7 cut(s)	36, 104, 626, 656, 696, 1702, 1792
PspPPI	RGGWCCY	1 cut(s)	1702
PsuI	RGATCY	5 cut(s)	330, 352, 1123, 1468, 2014
PsyI	GACNNNGTC	1 cut(s)	208
PvuII	CAGCTG	1 cut(s)	2162
RsaI	GTAC	5 cut(s)	42, 663, 1189, 1892, 2061
RsaNI	GTAC	5 cut(s)	41, 662, 1188, 1891, 2060
RseI	CAYNNNNRTG	5 cut(s)	417, 453, 771, 1818, 1929
Rsr2I	CGGWCCG	1 cut(s)	36
RsrII	CGGWCCG	1 cut(s)	36
SapI	GCTCTTC	1 cut(s)	401
SaqAI	TTAA	7 cut(s)	503, 864, 1358, 1385, 1422, 1566, 2141
SatI	GCNGC	9 cut(s)	264, 399, 798, 969, 1653, 1772, 1875, 2160, 2163
Sau96I	GGNCC	7 cut(s)	36, 104, 626, 656, 696, 1702, 1792
ScaI	AGTACT	1 cut(s)	2061
SchI	GAGTC	1 cut(s)	1986
ScrFI	CCNGG	5 cut(s)	345, 536, 1026, 1041, 1503
SduI	GDGCHC	4 cut(s)	521, 1214, 2134, 2301
SfaNI	GCATC	2 cut(s)	736, 2294
SfcI	CTRYAG	2 cut(s)	462, 665
SfuI	TTCGAA	4 cut(s)	491, 509, 638, 1117
SinI	GGWCC	4 cut(s)	36, 104, 656, 1702
SmiMI	CAYNNNNRTG	5 cut(s)	417, 453, 771, 1818, 1929
SmlI	CTYRAG	3 cut(s)	1512, 1886, 2213
SmoI	CTYRAG	3 cut(s)	1512, 1886, 2213
SsiI	CCGC	7 cut(s)	159, 234, 819, 1146, 1717, 1975, 2111
SspMI	CTAG	6 cut(s)	156, 830, 1077, 1179, 1613, 2066
StyD4I	CCNGG	5 cut(s)	343, 534, 1024, 1039, 1501
StyI	CCWWGG	1 cut(s)	1819
TaaI	ACNGT	4 cut(s)	463, 1676, 1724, 2177
TaiI	ACGT	1 cut(s)	209
TaqII	GACCGA	1 cut(s)	24
TatI	WGTACW	2 cut(s)	1187, 2059
TfiI	GAWTC	7 cut(s)	823, 877, 1167, 1297, 1492, 1554, 2071
Tru1I	TTAA	7 cut(s)	503, 864, 1358, 1385, 1422, 1566, 2141
Tru9I	TTAA	7 cut(s)	503, 864, 1358, 1385, 1422, 1566, 2141
TscAI	CASTG	3 cut(s)	1727, 2083, 2139
TseI	GCWGC	9 cut(s)	263, 398, 797, 968, 1652, 1771, 1874, 2159, 2162
TspDTI	ATGAA	4 cut(s)	465, 1173, 1897, 1913
TspGWI	ACGGA	6 cut(s)	28, 243, 325, 1477, 1511, 1566
TspRI	CASTG	3 cut(s)	1727, 2083, 2139
Tth111I	GACNNNGTC	1 cut(s)	208
VneI	GTGCAC	1 cut(s)	1210
VpaK11BI	GGWCC	4 cut(s)	36, 104, 656, 1702
VspI	ATTAAT	1 cut(s)	1358
XagI	CCTNNNNNAGG	1 cut(s)	1898
XapI	RAATTY	7 cut(s)	283, 477, 631, 640, 670, 711, 1904
XceI	RCATGY	1 cut(s)	1199
XmiI	GTMKAC	1 cut(s)	1779
XmnI	GAANNNNTTC	1 cut(s)	285
XspI	CTAG	6 cut(s)	156, 830, 1077, 1179, 1613, 2066
ZraI	GACGTC	1 cut(s)	207
ZrmI	AGTACT	1 cut(s)	2061

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.