Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0013846

Physical Location & Seq

Genomic Coordinates Reverse (-)

19281977 .. 19285003

Gene Structure 3027 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1120400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 801 bp

ATGAGCGTCATCGAAATCTTGACCAGAGTAGATGCGATCTGCCAGAAGTACGACCGATACGACGTCGAAAAGCAGAAGGATCTCAATGTCTCCGGCGACGACGCCTTTGCTCGCCTCTACGCCTCCGTCGAATCCGATATTGAAGCCGCCCTCCAGAAAGCGGAGATTGCTTCGAAAGAGAAAAATAGGGCATCTGCTGTTGCACTGAATGCTGAGATTCGTAGGACCAAGGCTCGATTACTCGAGGAGGTTCCCAAATTGCAGAGATTGGCTGTAAAGAAGGTTAAAGGGCTTTCATCCCAAGATTTTGCTGCTCGTAATGATTTGGTCCATGCATTGCCAGATAGGATCCAGGACATCCCAGATGGGACTCCAGCTGCACCCAAACAAACTGGAGGAGGTTGGGCAGCTTCAGCTTCTCGTACAGAAATCAAATTCGATTCTGAGGGACGGTTTGATAATGAATTCTTCCAACAGTCTGAGTCGTCGAGTAATTTCAGGCAAGAGTATGAAATGCGGAAAATGAAGCAGGATCAAGGTTTGGATGTGATATCAGATGGTTTGGATACGTTGAAAAATATGGCTCAGGATATGAACGAGGAATTCGATAGGCAAGTTCCTTTGATGGATGAGATTGACTCTAAGGTGGACAAGGCATCTGCGGACCTTAAGAATACCAATGTTAGACTGAAGGACACAGTCAACCAGCTGAGGTCCAGCCGAAATTTCTGTATTGATATTGTATTGCTGTGTATAATTTTGGGCATTGCTGCGTATTTGTACAATGTACTGAAGAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000149 GO:0003674 GO:0005215 GO:0005484 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0005911 GO:0006605 GO:0006612 GO:0006810 GO:0006886 GO:0006906 GO:0006996 GO:0008104 GO:0008150 GO:0008565 GO:0009506 GO:0009987 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016021 GO:0016043 GO:0016050 GO:0016192 GO:0022406 GO:0030054 GO:0031201 GO:0031224 GO:0032991 GO:0033036 GO:0034613 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0045184 GO:0046907 GO:0048278 GO:0048284 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051649 GO:0055044 GO:0061024 GO:0061025 GO:0070727 GO:0071702 GO:0071705 GO:0071840 GO:0071944 GO:0072657 GO:0090150 GO:0090174 GO:0098796 GO:0140056

KEGG Pathways

Metabolic & Signaling

ko04130 map04130

Pfam Domains

Protein Families

SNARE

Protein Analysis

267

Amino Acids

29.93

Weight (kDa)

5.36

Isoelectric Point (pI)

48.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SNARE	PF05739	208 - 257	3e-11	SNARE domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013846)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G09740
fragaria_vesca	FvH4_6g15660
malus_domestica	MD04G1100500.v1.1 MD12G1120400.v1.1
prunus_persica	Prupe.6G238700_v2.0.a1
pyrus_communis	pycom04g09550 pycom12g11940
rosa_chinensis	RchiOBHm_Chr3g0469361
rosa_laevigata	RLG00000024356
rosa_multiflora	Rmu_co8487771.1_g000001 Rmu_sc0013047.1_g000010
rosa_roxburghii	Rroxscaffold_6G00411960
rosa_rugosa	Rorug03G0100900
rosa_samantha	Rh3BG173800 Rh3CG164700 Rh3DG201700
rosa_wichuraiana	Rw3G014150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	66
AciI	CCGC	4 cut(s)	147, 161, 517, 662
AclWI	GGATC	4 cut(s)	87, 343, 356, 540
AcsI	RAATTY	4 cut(s)	434, 464, 602, 724
AcuI	CTGAAG	2 cut(s)	396, 710
AcyI	GRCGYC	2 cut(s)	63, 102
AfaI	GTAC	4 cut(s)	50, 424, 782, 789
AfiI	CCNNNNNNNGG	1 cut(s)	160
AflII	CTTAAG	1 cut(s)	668
AgsI	TTSAA	2 cut(s)	143, 574
AjnI	CCWGG	1 cut(s)	351
AluBI	AGCT	4 cut(s)	377, 410, 416, 709
AluI	AGCT	4 cut(s)	377, 410, 416, 709
Alw26I	GTCTC	1 cut(s)	94
AlwI	GGATC	4 cut(s)	87, 343, 356, 540
Ama87I	CYCGRG	1 cut(s)	242
ApeKI	GCWGC	4 cut(s)	311, 377, 407, 770
ApoI	RAATTY	4 cut(s)	434, 464, 602, 724
ArsI	GACNNNNNNTTYG	2 cut(s)	89, 121
AspS9I	GGNCC	4 cut(s)	225, 328, 664, 714
AsuII	TTCGAA	1 cut(s)	173
AvaI	CYCGRG	1 cut(s)	242
AvaII	GGWCC	4 cut(s)	225, 328, 664, 714
BamHI	GGATCC	1 cut(s)	348
BbvCI	CCTCAGC	1 cut(s)	710
BbvI	GCAGC	4 cut(s)	298, 364, 419, 757
BccI	CCATC	3 cut(s)	359, 551, 619
BcgI	CGANNNNNNTGC	2 cut(s)	89, 123
BciT130I	CCWGG	1 cut(s)	353
BciVI	GTATCC	1 cut(s)	559
BcoDI	GTCTC	1 cut(s)	94
BfrI	CTTAAG	1 cut(s)	668
BfuI	GTATCC	1 cut(s)	559
BisI	GCNGC	5 cut(s)	147, 312, 378, 408, 771
BlsI	GCNGC	5 cut(s)	148, 313, 379, 409, 772
Bme1390I	CCNGG	1 cut(s)	353
Bme18I	GGWCC	4 cut(s)	225, 328, 664, 714
BmeT110I	CYCGRG	1 cut(s)	242
BmgT120I	GGNCC	4 cut(s)	225, 328, 664, 714
BmiI	GGNNCC	2 cut(s)	252, 350
BmrFI	CCNGG	1 cut(s)	353
BmsI	GCATC	3 cut(s)	22, 200, 665
BplI	GAGNNNNNCTC	2 cut(s)	623, 655
BpmI	CTGGAG	3 cut(s)	137, 357, 414
Bpu10I	CCTNAGC	2 cut(s)	585, 710
Bpu14I	TTCGAA	1 cut(s)	173
BsaHI	GRCGYC	2 cut(s)	63, 102
BsaJI	CCNNGG	1 cut(s)	228
Bsc4I	CCNNNNNNNGG	1 cut(s)	160
Bse1I	ACTGG	1 cut(s)	397
Bse3DI	GCAATG	2 cut(s)	335, 765
BseBI	CCWGG	1 cut(s)	353
BseDI	CCNNGG	1 cut(s)	228
BseGI	GGATG	4 cut(s)	296, 357, 550, 634
BseLI	CCNNNNNNNGG	1 cut(s)	160
BseMI	GCAATG	2 cut(s)	335, 765
BseMII	CTCAG	5 cut(s)	204, 435, 471, 599, 701
BseNI	ACTGG	1 cut(s)	397
BseRI	GAGGAG	2 cut(s)	260, 411
BseXI	GCAGC	4 cut(s)	298, 364, 419, 757
BsgI	GTGCAG	1 cut(s)	363
Bsh1285I	CGRYCG	1 cut(s)	55
BsiEI	CGRYCG	1 cut(s)	55
BsiHKCI	CYCGRG	1 cut(s)	242
BsiSI	CCGG	1 cut(s)	93
BslFI	GGGAC	2 cut(s)	382, 462
BslI	CCNNNNNNNGG	1 cut(s)	160
BsmAI	GTCTC	1 cut(s)	94
BsmFI	GGGAC	2 cut(s)	382, 462
BsmI	GAATGC	1 cut(s)	214
BsoBI	CYCGRG	1 cut(s)	242
Bsp119I	TTCGAA	1 cut(s)	173
Bsp1407I	TGTACA	1 cut(s)	780
Bsp143I	GATC	4 cut(s)	36, 79, 348, 532
BspACI	CCGC	4 cut(s)	147, 161, 517, 662
BspCNI	CTCAG	5 cut(s)	205, 436, 472, 598, 702
BspLI	GGNNCC	2 cut(s)	252, 350
BspPI	GGATC	4 cut(s)	87, 343, 356, 540
BspT104I	TTCGAA	1 cut(s)	173
BspTI	CTTAAG	1 cut(s)	668
BsrDI	GCAATG	2 cut(s)	335, 765
BsrGI	TGTACA	1 cut(s)	780
BsrI	ACTGG	1 cut(s)	397
BssECI	CCNNGG	1 cut(s)	228
BssMI	GATC	4 cut(s)	36, 79, 348, 532
BssNI	GRCGYC	2 cut(s)	63, 102
BssT1I	CCWWGG	1 cut(s)	228
Bst2UI	CCWGG	1 cut(s)	353
Bst4CI	ACNGT	3 cut(s)	453, 477, 700
BstACI	GRCGYC	2 cut(s)	63, 102
BstAFI	CTTAAG	1 cut(s)	668
BstAPI	GCANNNNNTGC	1 cut(s)	209
BstAUI	TGTACA	1 cut(s)	780
BstBI	TTCGAA	1 cut(s)	173
BstC8I	GCNNGC	1 cut(s)	112
BstDEI	CTNAG	6 cut(s)	213, 444, 480, 585, 642, 710
BstF5I	GGATG	4 cut(s)	296, 357, 550, 634
BstKTI	GATC	4 cut(s)	39, 82, 351, 535
BstMAI	GTCTC	1 cut(s)	94
BstMBI	GATC	4 cut(s)	36, 79, 348, 532
BstMCI	CGRYCG	1 cut(s)	55
BstMWI	GCNNNNNNNGC	3 cut(s)	167, 209, 413
BstNI	CCWGG	1 cut(s)	353
BstSCI	CCNGG	1 cut(s)	351
BstV1I	GCAGC	4 cut(s)	298, 364, 419, 757
BstX2I	RGATCY	2 cut(s)	79, 348
BstYI	RGATCY	2 cut(s)	79, 348
BsuI	GTATCC	1 cut(s)	559
BtsCI	GGATG	4 cut(s)	296, 357, 550, 634
BtsIMutI	CAGTG	1 cut(s)	203
Cac8I	GCNNGC	1 cut(s)	112
Cfr13I	GGNCC	4 cut(s)	225, 328, 664, 714
CseI	GACGC	1 cut(s)	110
Csp6I	GTAC	4 cut(s)	49, 423, 781, 788
CviAII	CATG	1 cut(s)	332
CviQI	GTAC	4 cut(s)	49, 423, 781, 788
DdeI	CTNAG	6 cut(s)	213, 444, 480, 585, 642, 710
DpnI	GATC	4 cut(s)	38, 81, 350, 534
DpnII	GATC	4 cut(s)	36, 79, 348, 532
Eco130I	CCWWGG	1 cut(s)	228
Eco32I	GATATC	1 cut(s)	552
Eco47I	GGWCC	4 cut(s)	225, 328, 664, 714
Eco57I	CTGAAG	2 cut(s)	396, 710
Eco88I	CYCGRG	1 cut(s)	242
EcoRI	GAATTC	2 cut(s)	464, 602
EcoRII	CCWGG	1 cut(s)	351
EcoRV	GATATC	1 cut(s)	552
EcoT14I	CCWWGG	1 cut(s)	228
EcoT22I	ATGCAT	1 cut(s)	337
ErhI	CCWWGG	1 cut(s)	228
FaeI	CATG	1 cut(s)	335
FaiI	YATR	5 cut(s)	333, 510, 581, 593, 755
FaqI	GGGAC	2 cut(s)	382, 462
FatI	CATG	1 cut(s)	331
Fnu4HI	GCNGC	5 cut(s)	147, 312, 378, 408, 771
FokI	GGATG	4 cut(s)	283, 344, 557, 641
Fsp4HI	GCNGC	5 cut(s)	147, 312, 378, 408, 771
GluI	GCNGC	5 cut(s)	147, 312, 378, 408, 771
GsuI	CTGGAG	3 cut(s)	137, 357, 414
HapII	CCGG	1 cut(s)	93
HgaI	GACGC	1 cut(s)	110
Hin1I	GRCGYC	2 cut(s)	63, 102
Hin1II	CATG	1 cut(s)	335
HincII	GTYRAC	1 cut(s)	703
HindII	GTYRAC	1 cut(s)	703
HinfI	GANTC	6 cut(s)	131, 217, 370, 440, 482, 638
HpaII	CCGG	1 cut(s)	93
Hpy166II	GTNNAC	2 cut(s)	649, 703
Hpy188I	TCNGA	4 cut(s)	136, 445, 481, 556
Hpy188III	TCNNGA	3 cut(s)	19, 154, 587
Hpy8I	GTNNAC	2 cut(s)	649, 703
Hpy99I	CGWCG	6 cut(s)	65, 68, 101, 104, 131, 490
HpyAV	CCTTC	3 cut(s)	70, 274, 685
HpyCH4III	ACNGT	3 cut(s)	453, 477, 700
HpyCH4IV	ACGT	2 cut(s)	63, 569
HpyCH4V	TGCA	4 cut(s)	203, 262, 335, 380
HpyF10VI	GCNNNNNNNGC	3 cut(s)	167, 209, 413
HpyF3I	CTNAG	6 cut(s)	213, 444, 480, 585, 642, 710
HpySE526I	ACGT	2 cut(s)	63, 569
Hsp92I	GRCGYC	2 cut(s)	63, 102
Hsp92II	CATG	1 cut(s)	335
Kzo9I	GATC	4 cut(s)	36, 79, 348, 532
Lsp1109I	GCAGC	4 cut(s)	298, 364, 419, 757
LweI	GCATC	3 cut(s)	22, 200, 665
MaeII	ACGT	2 cut(s)	63, 569
MalI	GATC	4 cut(s)	38, 81, 350, 534
MboI	GATC	4 cut(s)	36, 79, 348, 532
MboII	GAAGA	1 cut(s)	460
MflI	RGATCY	2 cut(s)	79, 348
MluCI	AATT	7 cut(s)	257, 434, 464, 493, 602, 724, 756
MlyI	GAGTC	3 cut(s)	364, 491, 632
MmeI	TCCRAC	1 cut(s)	496
Mph1103I	ATGCAT	1 cut(s)	337
MseI	TTAA	2 cut(s)	285, 669
MspA1I	CMGCKG	2 cut(s)	377, 709
MspCI	CTTAAG	1 cut(s)	668
MspI	CCGG	1 cut(s)	93
MspR9I	CCNGG	1 cut(s)	353
Mva1269I	GAATGC	1 cut(s)	214
MvaI	CCWGG	1 cut(s)	353
MwoI	GCNNNNNNNGC	3 cut(s)	167, 209, 413
NdeII	GATC	4 cut(s)	36, 79, 348, 532
NlaIII	CATG	1 cut(s)	335
NlaIV	GGNNCC	2 cut(s)	252, 350
NsiI	ATGCAT	1 cut(s)	337
NspV	TTCGAA	1 cut(s)	173
PaeR7I	CTCGAG	1 cut(s)	242
PcsI	WCGNNNNNNNCGW	3 cut(s)	57, 126, 603
PctI	GAATGC	1 cut(s)	214
PfeI	GAWTC	3 cut(s)	131, 217, 440
PflFI	GACNNNGTC	1 cut(s)	698
PfoI	TCCNGGA	1 cut(s)	351
PkrI	GCNGC	5 cut(s)	148, 313, 379, 409, 772
PleI	GAGTC	3 cut(s)	364, 490, 632
PpsI	GAGTC	3 cut(s)	364, 490, 632
Psp6I	CCWGG	1 cut(s)	351
PspGI	CCWGG	1 cut(s)	351
PspN4I	GGNNCC	2 cut(s)	252, 350
PspPI	GGNCC	4 cut(s)	225, 328, 664, 714
PspXI	VCTCGAGB	1 cut(s)	242
PsuI	RGATCY	2 cut(s)	79, 348
PsyI	GACNNNGTC	1 cut(s)	698
PvuII	CAGCTG	2 cut(s)	377, 709
RsaI	GTAC	4 cut(s)	50, 424, 782, 789
RsaNI	GTAC	4 cut(s)	49, 423, 781, 788
SaqAI	TTAA	2 cut(s)	285, 669
SatI	GCNGC	5 cut(s)	147, 312, 378, 408, 771
Sau3AI	GATC	4 cut(s)	36, 79, 348, 532
Sau96I	GGNCC	4 cut(s)	225, 328, 664, 714
SchI	GAGTC	3 cut(s)	364, 491, 632
ScrFI	CCNGG	1 cut(s)	353
SfaNI	GCATC	3 cut(s)	22, 200, 665
Sfr274I	CTCGAG	1 cut(s)	242
SfuI	TTCGAA	1 cut(s)	173
SinI	GGWCC	4 cut(s)	225, 328, 664, 714
SlaI	CTCGAG	1 cut(s)	242
SmlI	CTYRAG	2 cut(s)	242, 668
SmoI	CTYRAG	2 cut(s)	242, 668
Sse9I	AATT	7 cut(s)	257, 434, 464, 493, 602, 724, 756
SsiI	CCGC	4 cut(s)	147, 161, 517, 662
StyD4I	CCNGG	1 cut(s)	351
StyI	CCWWGG	1 cut(s)	228
TaaI	ACNGT	3 cut(s)	453, 477, 700
TaiI	ACGT	2 cut(s)	66, 572
TaqI	TCGA	9 cut(s)	12, 66, 129, 173, 235, 243, 438, 488, 606
TaqII	GACCGA	1 cut(s)	69
TasI	AATT	7 cut(s)	257, 434, 464, 493, 602, 724, 756
TatI	WGTACW	2 cut(s)	780, 787
TauI	GCSGC	1 cut(s)	149
TfiI	GAWTC	3 cut(s)	131, 217, 440
Tru1I	TTAA	2 cut(s)	285, 669
Tru9I	TTAA	2 cut(s)	285, 669
TscAI	CASTG	1 cut(s)	210
TseI	GCWGC	4 cut(s)	311, 377, 407, 770
TspDTI	ATGAA	5 cut(s)	285, 477, 525, 539, 608
TspGWI	ACGGA	1 cut(s)	115
TspRI	CASTG	1 cut(s)	210
Tth111I	GACNNNGTC	1 cut(s)	698
Vha464I	CTTAAG	1 cut(s)	668
VpaK11BI	GGWCC	4 cut(s)	225, 328, 664, 714
XapI	RAATTY	4 cut(s)	434, 464, 602, 724
XhoI	CTCGAG	1 cut(s)	242
ZraI	GACGTC	1 cut(s)	64
Zsp2I	ATGCAT	1 cut(s)	337

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.