Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0016022

Physical Location & Seq

Genomic Coordinates Reverse (-)

21317556 .. 21319145

Gene Structure 1590 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1136500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1590 bp

ATGAAATCCCTAATTTGCAGGTCCCCTTTCTCTCACTTGATACCGAGGAAATCTCACACTTCAGCTTGTTCCCTTTTTACTGAAACCCAGAAAGCACATACCCATTCTCTGCAAGCAAGAAACTCGGATGCGTTCTGGCATGCACGCCAACTGTTCGACGAAACGCCTGCCTTGAGCGTGGTATCGGCCACAACAATAATAGGCCAATTTGCTCGGCAGCACCGTCACGAAGAAGCTATTTATCTCTTTTCTAGGATGCTTGTTTTAAGTATTATCCCCAACGAATTCACATTCGGCACTGTCATTCACTCGTCGACCGCACTCGGAGACCTATCAATAGGCAAGCAGCTTCATGCCTGTGCAACAAAAACGGGTTTGCGTTCGAACGTCTTTGTGGGGAGTGCAATTTTGGATCTTTATTCCAAGTTGAGCGTTAGCCAAGATGCTCAAAGAGCTTTCGAAGATACCCAGTACCCGAATGTGGTTTCTTACACGACGTTGATATGCGCATACCTGAAAAAGGAGAAGCTTGAAGATGCCCTGCTTCTTTTCCGAGGGATGCCGGTGAGGAATGTTGTTTCGTGGAATGCAATGATTGGAGGCTACAGCCAAACAGGCCACAATGAAGAGGCTGTAAATCTTTTCGCCGAGATGCTTAGAGATGGATTGGTGCCTACCCATTCTACTTTTCCATGTGCCATCATTGCAGCTGCCAACATAGCAGCACTAGGAATGGGCAGGAGCTTTCATGCCTGTGCTGTCAAGTTCTTGGGTAAGCTTGATGTTTTCATTGGCAATTCATTAATTAGCTTTTATGCAAAGTGTGGAAGCATGGAAGATAGTCTCTTAGTTTTCGTTAAACTTGAGGGAAAAAACATTGTTTCTTGGAATGCTGTGATATGTGGTTATGCTCAAAATGGTAAAGGAGAGGAAGCCATTGGATTTTTCGAAAGGATGAGGGTTTCTGGTTGCAGACCGAATAGCGTTACACTTCTTGGCTTATTGTGGGCTTGTAATCATGCTGGTCTTGTCGATGAAGGTTACTCATATTTCAATCAGGCAAGAACTGAGGACTCTAGCATTCTAAAGCCTGAGCACTATGCTTGTATGGTTGATTTACTCTCGCGCTCGGGGTGTTTCACACAAGCTGAGGAGTTCATCTGCAATCTACCTTTTGAACCGGGTATTGGATTTTGGAAGGCATTGCTTGGTGGCTGCCAAATCCACTCGAATACGGAATTGGGGGAGTTTGCGGCTAGGAAAATCCTGGATTTGGATCCAGAAGATGTGTCATCGTATGTTATGGTATCCAATGCACATTCTGCAGCTGGGAGATGGCAGAGTGTCTCAACAGTACGGAGAGAAATGAAAGAAAAAGGACTAAAGAGGGTTCCAGGCTGCAGTTGGATAGAAGTTAGGAACAAAGTTCATGTATTTGTTACTGGGGATAAGAATCATCCGCAATTGAATGAAATTTTTACCATGTTAAAAATTTTGTATAGAGCATTTAAGGGAGAAGTATGTAGACAGGCTCCAGCATCTCCAATCGATATGTCAAATTGCCACAAGGCTTCTAAGCTCTTGCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

530

Amino Acids

58.56

Weight (kDa)

8.37

Isoelectric Point (pI)

45.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	59 - 103	4.8e-07	PPR repeat family
PPR_2	PF13041	159 - 192	9.8e-07	PPR repeat family
PPR_2	PF13041	190 - 231	4.9e-11	PPR repeat family
PPR	PF01535	193 - 223	2.6e-08	PPR repeat
PPR_2	PF13041	291 - 335	6.2e-12	PPR repeat family
PPR	PF01535	294 - 324	3.7e-09	PPR repeat
E_motif	PF20431	409 - 471	7.3e-22	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016022)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G42450 AT5G42450
fragaria_vesca	FvH4_6g14440 FvH4_6g14560
malus_domestica	MD12G1136500.v1.1
prunus_persica	Prupe.6G248900_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0467611
rosa_laevigata	RLG00000024485
rosa_multiflora	Rmu_sc0011632.1_g000010
rosa_roxburghii	Rroxscaffold_6G00413550
rosa_rugosa	Rorug03G0088500
rosa_samantha	Rh3AG136800 Rh3BG158600 Rh3DG159100
rosa_wichuraiana	Rw3G012960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	508
Acc36I	ACCTGC	1 cut(s)	9
AccB1I	GGYRCC	1 cut(s)	670
AccI	GTMKAC	2 cut(s)	314, 1525
AccII	CGCG	1 cut(s)	1126
AciI	CCGC	3 cut(s)	318, 1253, 1460
AclWI	GGATC	3 cut(s)	420, 1271, 1284
AcoI	YGGCCR	1 cut(s)	186
AcsI	RAATTY	3 cut(s)	284, 1473, 1491
AcuI	CTGAAG	1 cut(s)	45
AfaI	GTAC	2 cut(s)	473, 1356
AfiI	CCNNNNNNNGG	4 cut(s)	481, 520, 1187, 1273
AgsI	TTSAA	4 cut(s)	533, 1054, 1178, 1468
AjnI	CCWGG	2 cut(s)	1266, 1393
AjuI	GAANNNNNNNTTGG	4 cut(s)	1170, 1202, 1223, 1255
Alw21I	GWGCWC	1 cut(s)	1098
Alw26I	GTCTC	3 cut(s)	321, 848, 1351
AlwI	GGATC	3 cut(s)	420, 1271, 1284
Ama87I	CYCGRG	1 cut(s)	1129
AoxI	GGCC	3 cut(s)	186, 202, 616
ApeKI	GCWGC	8 cut(s)	217, 346, 707, 710, 722, 1215, 1325, 1398
ApoI	RAATTY	3 cut(s)	284, 1473, 1491
AseI	ATTAAT	1 cut(s)	803
AspLEI	GCGC	2 cut(s)	509, 1128
AspS9I	GGNCC	1 cut(s)	21
AsuC2I	CCSGG	1 cut(s)	1182
AsuHPI	GGTGA	1 cut(s)	577
AsuII	TTCGAA	3 cut(s)	383, 459, 948
AvaI	CYCGRG	1 cut(s)	1129
AvaII	GGWCC	1 cut(s)	21
BamHI	GGATCC	1 cut(s)	1276
BanI	GGYRCC	1 cut(s)	670
Bbv12I	GWGCWC	1 cut(s)	1098
BbvCI	CCTCAGC	1 cut(s)	1149
BbvI	GCAGC	8 cut(s)	229, 358, 697, 719, 734, 1202, 1337, 1385
BccI	CCATC	3 cut(s)	656, 707, 1329
BcgI	CGANNNNNNTGC	4 cut(s)	105, 139, 149, 183
BciT130I	CCWGG	2 cut(s)	1268, 1395
BciVI	GTATCC	1 cut(s)	1318
BcnI	CCSGG	1 cut(s)	1182
BcoDI	GTCTC	3 cut(s)	321, 848, 1351
BfaI	CTAG	4 cut(s)	252, 728, 1077, 1257
BfmI	CTRYAG	3 cut(s)	604, 1323, 1399
BfuAI	ACCTGC	1 cut(s)	9
BfuI	GTATCC	1 cut(s)	1318
BglI	GCCNNNNNGGC	1 cut(s)	615
BisI	GCNGC	9 cut(s)	218, 347, 708, 711, 723, 1216, 1254, 1326, 1399
BlsI	GCNGC	9 cut(s)	219, 348, 709, 712, 724, 1217, 1255, 1327, 1400
Bme1390I	CCNGG	3 cut(s)	1182, 1268, 1395
Bme18I	GGWCC	1 cut(s)	21
BmeT110I	CYCGRG	1 cut(s)	1129
BmgT120I	GGNCC	1 cut(s)	21
BmiI	GGNNCC	5 cut(s)	23, 672, 1278, 1392, 1533
BmrFI	CCNGG	3 cut(s)	1182, 1268, 1395
BmrI	ACTGGG	2 cut(s)	463, 1452
BmsI	GCATC	7 cut(s)	118, 246, 433, 526, 549, 642, 1547
BmuI	ACTGGG	2 cut(s)	463, 1452
BplI	GAGNNNNNCTC	2 cut(s)	37, 69
BpmI	CTGGAG	1 cut(s)	1518
Bpu10I	CCTNAGC	2 cut(s)	1092, 1149
Bpu14I	TTCGAA	3 cut(s)	383, 459, 948
BpuEI	CTTGAG	2 cut(s)	193, 884
BpuMI	CCSGG	1 cut(s)	1182
Bsa29I	ATCGAT	1 cut(s)	1548
BsaBI	GATNNNNATC	2 cut(s)	1275, 1452
BsaI	GGTCTC	1 cut(s)	321
BsaJI	CCNNGG	2 cut(s)	44, 553
Bsc4I	CCNNNNNNNGG	4 cut(s)	481, 520, 1187, 1273
Bse118I	RCCGGY	1 cut(s)	562
Bse1I	ACTGG	2 cut(s)	469, 1447
Bse3DI	GCAATG	3 cut(s)	597, 702, 1202
Bse8I	GATNNNNATC	2 cut(s)	1275, 1452
BseBI	CCWGG	2 cut(s)	1268, 1395
BseCI	ATCGAT	1 cut(s)	1548
BseDI	CCNNGG	2 cut(s)	44, 553
BseGI	GGATG	5 cut(s)	133, 261, 564, 960, 1456
BseJI	GATNNNNATC	2 cut(s)	1275, 1452
BseLI	CCNNNNNNNGG	4 cut(s)	481, 520, 1187, 1273
BseMI	GCAATG	3 cut(s)	597, 702, 1202
BseMII	CTCAG	3 cut(s)	1059, 1083, 1140
BseNI	ACTGG	2 cut(s)	469, 1447
BseRI	GAGGAG	1 cut(s)	1166
BseXI	GCAGC	8 cut(s)	229, 358, 697, 719, 734, 1202, 1337, 1385
BseYI	CCCAGC	1 cut(s)	1328
Bsh1236I	CGCG	1 cut(s)	1126
Bsh1285I	CGRYCG	1 cut(s)	318
BshFI	GGCC	3 cut(s)	188, 204, 618
BshNI	GGYRCC	1 cut(s)	670
BshVI	ATCGAT	1 cut(s)	1548
BsiEI	CGRYCG	1 cut(s)	318
BsiHKAI	GWGCWC	1 cut(s)	1098
BsiHKCI	CYCGRG	1 cut(s)	1129
BsiSI	CCGG	2 cut(s)	563, 1181
BslFI	GGGAC	1 cut(s)	7
BslI	CCNNNNNNNGG	4 cut(s)	481, 520, 1187, 1273
BsmAI	GTCTC	3 cut(s)	321, 848, 1351
BsmFI	GGGAC	1 cut(s)	7
BsmI	GAATGC	3 cut(s)	592, 895, 1080
BsnI	GGCC	3 cut(s)	188, 204, 618
Bso31I	GGTCTC	1 cut(s)	321
BsoBI	CYCGRG	1 cut(s)	1129
Bsp119I	TTCGAA	3 cut(s)	383, 459, 948
Bsp1286I	GDGCHC	1 cut(s)	1098
Bsp143I	GATC	2 cut(s)	412, 1276
BspACI	CCGC	3 cut(s)	318, 1253, 1460
BspANI	GGCC	3 cut(s)	188, 204, 618
BspCNI	CTCAG	3 cut(s)	1060, 1084, 1141
BspDI	ATCGAT	1 cut(s)	1548
BspFNI	CGCG	1 cut(s)	1126
BspLI	GGNNCC	5 cut(s)	23, 672, 1278, 1392, 1533
BspMAI	CTGCAG	2 cut(s)	1327, 1403
BspMI	ACCTGC	1 cut(s)	9
BspPI	GGATC	3 cut(s)	420, 1271, 1284
BspT104I	TTCGAA	3 cut(s)	383, 459, 948
BspT107I	GGYRCC	1 cut(s)	670
BspTNI	GGTCTC	1 cut(s)	321
BsrDI	GCAATG	3 cut(s)	597, 702, 1202
BsrFI	RCCGGY	1 cut(s)	562
BsrI	ACTGG	2 cut(s)	469, 1447
BssAI	RCCGGY	1 cut(s)	562
BssECI	CCNNGG	2 cut(s)	44, 553
BssMI	GATC	2 cut(s)	412, 1276
Bst2UI	CCWGG	2 cut(s)	1268, 1395
Bst4CI	ACNGT	4 cut(s)	153, 224, 301, 1354
Bst6I	CTCTTC	1 cut(s)	621
BstAPI	GCANNNNNTGC	1 cut(s)	1322
BstBI	TTCGAA	3 cut(s)	383, 459, 948
BstC8I	GCNNGC	5 cut(s)	114, 141, 145, 168, 344
BstDEI	CTNAG	6 cut(s)	656, 847, 1068, 1092, 1149, 1575
BstENI	CCTNNNNNAGG	1 cut(s)	518
BstF5I	GGATG	5 cut(s)	133, 261, 564, 960, 1456
BstFNI	CGCG	1 cut(s)	1126
BstHHI	GCGC	2 cut(s)	509, 1128
BstKTI	GATC	2 cut(s)	415, 1279
BstMAI	GTCTC	3 cut(s)	321, 848, 1351
BstMBI	GATC	2 cut(s)	412, 1276
BstMCI	CGRYCG	1 cut(s)	318
BstMWI	GCNNNNNNNGC	5 cut(s)	452, 615, 704, 719, 1322
BstNI	CCWGG	2 cut(s)	1268, 1395
BstNSI	RCATGY	1 cut(s)	143
BstSCI	CCNGG	3 cut(s)	1180, 1266, 1393
BstSFI	CTRYAG	3 cut(s)	604, 1323, 1399
BstUI	CGCG	1 cut(s)	1126
BstV1I	GCAGC	8 cut(s)	229, 358, 697, 719, 734, 1202, 1337, 1385
BstX2I	RGATCY	2 cut(s)	412, 1276
BstYI	RGATCY	2 cut(s)	412, 1276
Bsu15I	ATCGAT	1 cut(s)	1548
BsuI	GTATCC	1 cut(s)	1318
BsuRI	GGCC	3 cut(s)	188, 204, 618
BsuTUI	ATCGAT	1 cut(s)	1548
BtsCI	GGATG	5 cut(s)	133, 261, 564, 960, 1456
BtsIMutI	CAGTG	1 cut(s)	297
BveI	ACCTGC	1 cut(s)	9
Cac8I	GCNNGC	5 cut(s)	114, 141, 145, 168, 344
CfoI	GCGC	2 cut(s)	509, 1128
Cfr10I	RCCGGY	1 cut(s)	562
Cfr13I	GGNCC	1 cut(s)	21
ClaI	ATCGAT	1 cut(s)	1548
Csp6I	GTAC	2 cut(s)	472, 1355
CviAII	CATG	8 cut(s)	140, 353, 693, 749, 832, 1019, 1430, 1483
CviQI	GTAC	2 cut(s)	472, 1355
DdeI	CTNAG	6 cut(s)	656, 847, 1068, 1092, 1149, 1575
DpnI	GATC	2 cut(s)	414, 1278
DpnII	GATC	2 cut(s)	412, 1276
EaeI	YGGCCR	1 cut(s)	186
Eam1104I	CTCTTC	1 cut(s)	621
EarI	CTCTTC	1 cut(s)	621
Eco31I	GGTCTC	1 cut(s)	321
Eco47I	GGWCC	1 cut(s)	21
Eco57I	CTGAAG	1 cut(s)	45
Eco88I	CYCGRG	1 cut(s)	1129
EcoNI	CCTNNNNNAGG	1 cut(s)	518
EcoO109I	RGGNCCY	1 cut(s)	21
EcoRI	GAATTC	1 cut(s)	284
EcoRII	CCWGG	2 cut(s)	1266, 1393
FaeI	CATG	8 cut(s)	143, 356, 696, 752, 835, 1022, 1433, 1486
FaqI	GGGAC	1 cut(s)	7
FatI	CATG	8 cut(s)	139, 352, 692, 748, 831, 1018, 1429, 1482
FblI	GTMKAC	2 cut(s)	314, 1525
Fnu4HI	GCNGC	9 cut(s)	218, 347, 708, 711, 723, 1216, 1254, 1326, 1399
FokI	GGATG	5 cut(s)	140, 268, 571, 967, 1443
Fsp4HI	GCNGC	9 cut(s)	218, 347, 708, 711, 723, 1216, 1254, 1326, 1399
FspAI	RTGCGCAY	1 cut(s)	508
FspBI	CTAG	4 cut(s)	252, 728, 1077, 1257
FspI	TGCGCA	1 cut(s)	508
GlaI	GCGC	2 cut(s)	508, 1127
GluI	GCNGC	9 cut(s)	218, 347, 708, 711, 723, 1216, 1254, 1326, 1399
GsaI	CCCAGC	1 cut(s)	1332
GsuI	CTGGAG	1 cut(s)	1518
HaeIII	GGCC	3 cut(s)	188, 204, 618
HapII	CCGG	2 cut(s)	563, 1181
HhaI	GCGC	2 cut(s)	509, 1128
Hin1II	CATG	8 cut(s)	143, 356, 696, 752, 835, 1022, 1433, 1486
Hin6I	GCGC	2 cut(s)	507, 1126
HinP1I	GCGC	2 cut(s)	507, 1126
HincII	GTYRAC	1 cut(s)	315
HindII	GTYRAC	1 cut(s)	315
HindIII	AAGCTT	2 cut(s)	527, 776
HinfI	GANTC	2 cut(s)	1073, 1453
HpaII	CCGG	2 cut(s)	563, 1181
HphI	GGTGA	1 cut(s)	577
Hpy166II	GTNNAC	2 cut(s)	315, 1526
Hpy188I	TCNGA	3 cut(s)	127, 326, 554
Hpy188III	TCNNGA	2 cut(s)	227, 1280
Hpy8I	GTNNAC	2 cut(s)	315, 1526
Hpy99I	CGWCG	3 cut(s)	161, 316, 499
HpyAV	CCTTC	2 cut(s)	1031, 1192
HpyCH4III	ACNGT	4 cut(s)	153, 224, 301, 1354
HpyCH4IV	ACGT	2 cut(s)	387, 497
HpyF10VI	GCNNNNNNNGC	5 cut(s)	452, 615, 704, 719, 1322
HpyF3I	CTNAG	6 cut(s)	656, 847, 1068, 1092, 1149, 1575
HpySE526I	ACGT	2 cut(s)	387, 497
Hsp92II	CATG	8 cut(s)	143, 356, 696, 752, 835, 1022, 1433, 1486
HspAI	GCGC	2 cut(s)	507, 1126
Kzo9I	GATC	2 cut(s)	412, 1276
LmnI	GCTCC	2 cut(s)	741, 1537
Lsp1109I	GCAGC	8 cut(s)	229, 358, 697, 719, 734, 1202, 1337, 1385
LweI	GCATC	7 cut(s)	118, 246, 433, 526, 549, 642, 1547
MaeI	CTAG	4 cut(s)	252, 728, 1077, 1257
MaeII	ACGT	2 cut(s)	387, 497
MaeIII	GTNAC	4 cut(s)	224, 985, 1040, 1438
MalI	GATC	2 cut(s)	414, 1278
MboI	GATC	2 cut(s)	412, 1276
MboII	GAAGA	6 cut(s)	242, 473, 545, 638, 848, 1295
MfeI	CAATTG	1 cut(s)	1463
MflI	RGATCY	2 cut(s)	412, 1276
MhlI	GDGCHC	1 cut(s)	1098
MlyI	GAGTC	1 cut(s)	1067
MmeI	TCCRAC	1 cut(s)	1385
MseI	TTAA	5 cut(s)	266, 803, 858, 1487, 1509
MslI	CAYNNNNRTG	2 cut(s)	357, 753
MspA1I	CMGCKG	2 cut(s)	710, 1328
MspI	CCGG	2 cut(s)	563, 1181
MspR9I	CCNGG	3 cut(s)	1182, 1268, 1395
MunI	CAATTG	1 cut(s)	1463
Mva1269I	GAATGC	3 cut(s)	592, 895, 1080
MvaI	CCWGG	2 cut(s)	1268, 1395
MvnI	CGCG	1 cut(s)	1126
MwoI	GCNNNNNNNGC	5 cut(s)	452, 615, 704, 719, 1322
NciI	CCSGG	1 cut(s)	1182
NdeII	GATC	2 cut(s)	412, 1276
NlaIII	CATG	8 cut(s)	143, 356, 696, 752, 835, 1022, 1433, 1486
NlaIV	GGNNCC	5 cut(s)	23, 672, 1278, 1392, 1533
NmeAIII	GCCGAG	2 cut(s)	193, 673
NmuCI	GTSAC	1 cut(s)	224
NsbI	TGCGCA	1 cut(s)	508
NspI	RCATGY	1 cut(s)	143
NspV	TTCGAA	3 cut(s)	383, 459, 948
PaeI	GCATGC	1 cut(s)	143
PcsI	WCGNNNNNNNCGW	1 cut(s)	220
PctI	GAATGC	3 cut(s)	592, 895, 1080
PfeI	GAWTC	1 cut(s)	1453
PfoI	TCCNGGA	1 cut(s)	1266
PkrI	GCNGC	9 cut(s)	219, 348, 709, 712, 724, 1217, 1255, 1327, 1400
PleI	GAGTC	1 cut(s)	1067
PpsI	GAGTC	1 cut(s)	1067
PpuMI	RGGWCCY	1 cut(s)	21
PshBI	ATTAAT	1 cut(s)	803
Psp5II	RGGWCCY	1 cut(s)	21
Psp6I	CCWGG	2 cut(s)	1266, 1393
PspFI	CCCAGC	1 cut(s)	1328
PspGI	CCWGG	2 cut(s)	1266, 1393
PspN4I	GGNNCC	5 cut(s)	23, 672, 1278, 1392, 1533
PspPI	GGNCC	1 cut(s)	21
PspPPI	RGGWCCY	1 cut(s)	21
PstI	CTGCAG	2 cut(s)	1327, 1403
PsuI	RGATCY	2 cut(s)	412, 1276
PvuII	CAGCTG	2 cut(s)	710, 1328
RsaI	GTAC	2 cut(s)	473, 1356
RsaNI	GTAC	2 cut(s)	472, 1355
RseI	CAYNNNNRTG	2 cut(s)	357, 753
SalI	GTCGAC	1 cut(s)	313
SaqAI	TTAA	5 cut(s)	266, 803, 858, 1487, 1509
SatI	GCNGC	9 cut(s)	218, 347, 708, 711, 723, 1216, 1254, 1326, 1399
Sau3AI	GATC	2 cut(s)	412, 1276
Sau96I	GGNCC	1 cut(s)	21
SchI	GAGTC	1 cut(s)	1067
ScrFI	CCNGG	3 cut(s)	1182, 1268, 1395
SduI	GDGCHC	1 cut(s)	1098
SfaNI	GCATC	7 cut(s)	118, 246, 433, 526, 549, 642, 1547
SfcI	CTRYAG	3 cut(s)	604, 1323, 1399
SfuI	TTCGAA	3 cut(s)	383, 459, 948
SinI	GGWCC	1 cut(s)	21
SmiMI	CAYNNNNRTG	2 cut(s)	357, 753
SmlI	CTYRAG	2 cut(s)	172, 863
SmoI	CTYRAG	2 cut(s)	172, 863
SphI	GCATGC	1 cut(s)	143
SsiI	CCGC	3 cut(s)	318, 1253, 1460
SspMI	CTAG	4 cut(s)	252, 728, 1077, 1257
StyD4I	CCNGG	3 cut(s)	1180, 1266, 1393
TaaI	ACNGT	4 cut(s)	153, 224, 301, 1354
TaiI	ACGT	2 cut(s)	390, 500
TaqI	TCGA	8 cut(s)	156, 314, 383, 459, 948, 1032, 1229, 1548
TaqII	GACCGA	1 cut(s)	991
TauI	GCSGC	1 cut(s)	1256
TfiI	GAWTC	1 cut(s)	1453
Tru1I	TTAA	5 cut(s)	266, 803, 858, 1487, 1509
Tru9I	TTAA	5 cut(s)	266, 803, 858, 1487, 1509
TscAI	CASTG	1 cut(s)	304
TseFI	GTSAC	1 cut(s)	224
TseI	GCWGC	8 cut(s)	217, 346, 707, 710, 722, 1215, 1325, 1398
Tsp45I	GTSAC	1 cut(s)	224
TspGWI	ACGGA	2 cut(s)	1250, 1372
TspRI	CASTG	1 cut(s)	304
VpaK11BI	GGWCC	1 cut(s)	21
VspI	ATTAAT	1 cut(s)	803
XagI	CCTNNNNNAGG	1 cut(s)	518
XapI	RAATTY	3 cut(s)	284, 1473, 1491
XceI	RCATGY	1 cut(s)	143
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1401
XmiI	GTMKAC	2 cut(s)	314, 1525
XspI	CTAG	4 cut(s)	252, 728, 1077, 1257

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.