Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0010365

Physical Location & Seq

Genomic Coordinates Reverse (-)

23360752 .. 23365561

Gene Structure 4810 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1153600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1860 bp

ATGGCTGAGGGTTTCGAACCCTACCATGTGCCCCAACAGAGCAGAAGGGACAAGCTCCGGGTCCTGGCGGGTCAAGCCCAGCCGGATTGCCTCCAACCCGCATCACCATCTCCTCCTCCACCACATCTACACCATGCTTTGCTCCCTCTCTCATACGATCCCACCACCTCTTTCATCTCCTCCTCCTCAGATTTGCTAATGTGCGCCGAGCCGGCCGTGAAAGAAGAGGGTAGTCATCATCACGTGATCAACCACTTGATGGGTTTTGCTTCCAATTCCACCTCCTCTTCTACTTTTCGCCACAACAACCACCACCATCCCTACATGGATCCACAATTCCCTCTCACTCTTACCGCCGTTCAAGAAATCAACGCCAACCCATTTCTCTGTCCCCCTCAAGACCTCCACCCCCTCAGAGATTTCGACCACCAACAACCTCCCTATGACGATCAAGTCGTCGTCTTCAAACCAGAGCCTCTCTCTCTGTCCCTCTCTTCTTCCCAACCCACCAACCACACCCACTACACCCAGCAAACCCAGAACAACACAAACAACAACAGTCTCCAGAGATTTTCCTCTCATGGGATTAATTATGATTCAATAAATTCTGGCGTTAGTGTTGCTTCTGTCCCTTTTGGACCGTTCACGGGGTACGCTTCGATTCTGAAGGGATCTAGGTTTTTGAAGCCGGCGCAGCAGTTGCTGGAGGAGTTTTGCGATGTGAGCAGTCGTGAAATTTACAGCGCGAAAGTCTTGACGGCCGATGATTCCTCTTCGTTTCTTGACCCTCCTATTGAGAGCTTTGGTGACGACGACTCACTGACGGCTGGGGGAGACGGCGGCCAGAGTACAAGAAAAAAGTCCAGACTAATTTCCATGCTCGACGAGGTTTATAGGAGGTACAAGCAATACTGTCAACAGATGCAGGCAGTGGCAAGGTCATTTGAGTATGTTGCTGGGCTTGGAAATGCAGCTCCTTATGCAAACTTAGCGATAAAAGCTATGGCTAGACATTTTAAGTGTTTGAAGAATGCTATCACTGATCAGCTCCACTCCGGAAATCGGAATGGTACTCATTTAAGACATGAAAAAGATGAAAATCCTATGCTTCATAGCAGTAGAAGTATATATGGCCAGAGATCCATTCATACCTCTGAGTTTGTGGATCACCAACCTGTTTGGAGACCACAAAGGGGTCTTCCTGAGCGCGCTGTTACTGTTCTTAGAGCCTGGTTGTTTGAACACTTCCTGCATCCTTATCCTACCGACTCGGATAAATTAATGTTGGCTAAACAAACTGGTCTATCACGAAGCCAGGTTTCAAATTGGTTTATTAATGCAAGAGTAAGGCTATGGAAACCAATGGTGGAAGAAATACACATGCTAGAAACACGGCAAGCCCAAAAAGCTTCACAAAGGGTGGATTTAAGTTCCAATAGATCAAGTGATGATCATTTACCTTCCCCAAACTCCCTGGGATCCGAGAATCCATCCACATCCACCCATAGAGTTCAAGACACCGCATCTAAGCGCACCAGAAGCGAACTTCATGATCATATCACTATGGGGGCTACTGAACAAAGCCCATCTTACAGTAATTTTCCAAGCCATATGCATGTAGGTCATGTTGGTATGAACATGGCAGGGGGGAGTAGTAGTGGTGTGTCTCTAACTCTGGGACTTCACCAAAATAATGGCATTGGTTTTTCAGCTCCCTTTCCTATCAATGCAGCTGAACGTTTTGGCCTTGGCCTTGACGGCAATAGCGAGAACTATGCAATGGTTGGTTTCGAAGCTCAAAATCGGCATTATGGAAGGGATGTCATGGGAGGGCAACTTTTGCACGATTTTGTGGGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001763 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0007275 GO:0007389 GO:0008150 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009908 GO:0010016 GO:0010051 GO:0010073 GO:0010074 GO:0010076 GO:0010077 GO:0010087 GO:0010089 GO:0010154 GO:0010223 GO:0010228 GO:0010346 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0019827 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044444 GO:0044464 GO:0045892 GO:0045934 GO:0048367 GO:0048438 GO:0048439 GO:0048457 GO:0048507 GO:0048519 GO:0048523 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0060255 GO:0061458 GO:0065007 GO:0080006 GO:0080090 GO:0090567 GO:0090698 GO:0097159 GO:0098727 GO:0140110 GO:1901363 GO:1902679 GO:1903506 GO:1903507 GO:1905393 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Homeobox_KN POX

Protein Analysis

620

Amino Acids

68.85

Weight (kDa)

6.54

Isoelectric Point (pI)

57.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
POX	PF07526	221 - 347	7.6e-38	Associated with HOX
Homeobox_KN	PF05920	411 - 450	1.1e-19	Homeobox KN domain
Homeodomain	PF00046	418 - 450	1.1e-06	Homeodomain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010365)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G02030
fragaria_vesca	FvH4_6g12810 FvH4_6g12810 FvH4_6g12810 FvH4_6g12810
malus_domestica	MD04G1138100.v1.1 MD12G1153600.v1.1
prunus_persica	Prupe.6G263900_v2.0.a1 Prupe.6G263900_v2.0.a1 Prupe.6G263900_v2.0.a1
pyrus_communis	pycom04g12630 pycom12g14630
rosa_chinensis	RchiOBHm_Chr3g0464821
rosa_laevigata	RLG00000024672
rosa_multiflora	Rmu_sc0007114.1_g000006
rosa_roxburghii	Rroxscaffold_6G00415420
rosa_rugosa	Rorug03G0074100.1
rosa_samantha	Rh3BG136800 Rh3CG139400 Rh3DG138200
rosa_wichuraiana	Rw3G011180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	452
AccB7I	CCANNNNNTGG	1 cut(s)	259
AccII	CGCG	2 cut(s)	746, 1209
AccIII	TCCGGA	1 cut(s)	1055
AciI	CCGC	5 cut(s)	68, 99, 354, 840, 1521
AclI	AACGTT	1 cut(s)	1738
AclWI	GGATC	8 cut(s)	152, 323, 336, 679, 1134, 1173, 1473, 1486
AcoI	YGGCCR	4 cut(s)	213, 759, 841, 1132
AcsI	RAATTY	2 cut(s)	604, 735
AcuI	CTGAAG	1 cut(s)	686
AcvI	CACGTG	1 cut(s)	244
AfaI	GTAC	4 cut(s)	653, 850, 902, 1072
AfiI	CCNNNNNNNGG	6 cut(s)	64, 259, 582, 647, 1062, 1193
AgsI	TTSAA	8 cut(s)	362, 466, 600, 685, 1027, 1241, 1323, 1514
AjnI	CCWGG	4 cut(s)	63, 1229, 1314, 1473
AluBI	AGCT	9 cut(s)	55, 801, 974, 1001, 1048, 1409, 1712, 1733, 1796
AluI	AGCT	9 cut(s)	55, 801, 974, 1001, 1048, 1409, 1712, 1733, 1796
Alw26I	GTCTC	4 cut(s)	566, 828, 1177, 1671
AlwI	GGATC	8 cut(s)	152, 323, 336, 679, 1134, 1173, 1473, 1486
AlwNI	CAGNNNCTG	1 cut(s)	703
Aor13HI	TCCGGA	1 cut(s)	1055
AoxI	GGCC	6 cut(s)	213, 759, 841, 1132, 1744, 1750
ApeKI	GCWGC	3 cut(s)	694, 971, 1730
ApoI	RAATTY	2 cut(s)	604, 735
AseI	ATTAAT	3 cut(s)	588, 1280, 1335
AspLEI	GCGC	6 cut(s)	206, 694, 746, 1209, 1211, 1533
AspS9I	GGNCC	2 cut(s)	61, 638
AsuC2I	CCSGG	1 cut(s)	59
AsuHPI	GGTGA	4 cut(s)	96, 818, 1160, 1676
AsuII	TTCGAA	2 cut(s)	15, 1791
AvaII	GGWCC	2 cut(s)	61, 638
BaeGI	GKGCMC	1 cut(s)	33
BalI	TGGCCA	1 cut(s)	1134
BamHI	GGATCC	2 cut(s)	328, 1478
BbrPI	CACGTG	1 cut(s)	244
BbsI	GAAGAC	2 cut(s)	454, 1190
BbvCI	CCTCAGC	1 cut(s)	6
BbvI	GCAGC	3 cut(s)	706, 983, 1742
BccI	CCATC	5 cut(s)	115, 253, 324, 1498, 1594
BceAI	ACGGC	7 cut(s)	200, 341, 774, 840, 853, 1409, 1774
BcgI	CGANNNNNNTGC	2 cut(s)	1757, 1791
BciT130I	CCWGG	4 cut(s)	65, 1231, 1316, 1475
BclI	TGATCA	4 cut(s)	246, 1042, 1450, 1552
BcnI	CCSGG	1 cut(s)	59
BcoDI	GTCTC	4 cut(s)	566, 828, 1177, 1671
BfaI	CTAG	3 cut(s)	675, 1008, 1385
BglI	GCCNNNNNGGC	2 cut(s)	212, 1758
BisI	GCNGC	4 cut(s)	695, 841, 972, 1731
BlsI	GCNGC	4 cut(s)	696, 842, 973, 1732
Bme1390I	CCNGG	5 cut(s)	59, 65, 1231, 1316, 1475
Bme18I	GGWCC	2 cut(s)	61, 638
BmgT120I	GGNCC	2 cut(s)	61, 638
BmiI	GGNNCC	3 cut(s)	62, 330, 1480
BmrFI	CCNGG	5 cut(s)	59, 65, 1231, 1316, 1475
BmsI	GCATC	4 cut(s)	110, 912, 1261, 1532
BpiI	GAAGAC	2 cut(s)	454, 1190
BplI	GAGNNNNNCTC	2 cut(s)	464, 496
BpmI	CTGGAG	2 cut(s)	548, 725
Bpu10I	CCTNAGC	2 cut(s)	6, 1203
Bpu14I	TTCGAA	2 cut(s)	15, 1791
BpuEI	CTTGAG	1 cut(s)	381
BpuMI	CCSGG	1 cut(s)	59
BsaAI	YACGTR	1 cut(s)	244
BsaBI	GATNNNNATC	1 cut(s)	1098
BsaI	GGTCTC	1 cut(s)	1177
BsaJI	CCNNGG	3 cut(s)	1473, 1474, 1747
BsaWI	WCCGGW	1 cut(s)	1055
BsaXI	ACNNNNNCTCC	2 cut(s)	1642, 1672
Bsc4I	CCNNNNNNNGG	6 cut(s)	64, 259, 582, 647, 1062, 1193
Bse118I	RCCGGY	2 cut(s)	211, 688
Bse1I	ACTGG	1 cut(s)	1303
Bse3DI	GCAATG	1 cut(s)	1785
Bse8I	GATNNNNATC	1 cut(s)	1098
BseAI	TCCGGA	1 cut(s)	1055
BseBI	CCWGG	4 cut(s)	65, 1231, 1316, 1475
BseDI	CCNNGG	3 cut(s)	1473, 1474, 1747
BseGI	GGATG	5 cut(s)	316, 1252, 1490, 1496, 1825
BseJI	GATNNNNATC	1 cut(s)	1098
BseLI	CCNNNNNNNGG	6 cut(s)	64, 259, 582, 647, 1062, 1193
BseMI	GCAATG	1 cut(s)	1785
BseMII	CTCAG	4 cut(s)	201, 427, 1146, 1194
BseNI	ACTGG	1 cut(s)	1303
BsePI	GCGCGC	1 cut(s)	1207
BseRI	GAGGAG	7 cut(s)	102, 105, 169, 172, 175, 274, 722
BseSI	GKGCMC	1 cut(s)	33
BseX3I	CGGCCG	2 cut(s)	213, 759
BseXI	GCAGC	3 cut(s)	706, 983, 1742
BseYI	CCCAGC	4 cut(s)	78, 528, 827, 956
Bsh1236I	CGCG	2 cut(s)	746, 1209
Bsh1285I	CGRYCG	2 cut(s)	216, 762
BshFI	GGCC	6 cut(s)	215, 761, 843, 1134, 1746, 1752
BsiEI	CGRYCG	2 cut(s)	216, 762
BsiSI	CCGG	5 cut(s)	58, 83, 212, 689, 1056
BslFI	GGGAC	5 cut(s)	62, 375, 472, 614, 1692
BslI	CCNNNNNNNGG	6 cut(s)	64, 259, 582, 647, 1062, 1193
BsmAI	GTCTC	4 cut(s)	566, 828, 1177, 1671
BsmBI	CGTCTC	1 cut(s)	828
BsmFI	GGGAC	5 cut(s)	62, 375, 472, 614, 1692
BsmI	GAATGC	1 cut(s)	1036
BsnI	GGCC	6 cut(s)	215, 761, 843, 1134, 1746, 1752
Bso31I	GGTCTC	1 cut(s)	1177
Bsp119I	TTCGAA	2 cut(s)	15, 1791
Bsp1286I	GDGCHC	1 cut(s)	33
Bsp13I	TCCGGA	1 cut(s)	1055
BspACI	CCGC	5 cut(s)	68, 99, 354, 840, 1521
BspANI	GGCC	6 cut(s)	215, 761, 843, 1134, 1746, 1752
BspCNI	CTCAG	4 cut(s)	200, 426, 1147, 1195
BspEI	TCCGGA	1 cut(s)	1055
BspFNI	CGCG	2 cut(s)	746, 1209
BspHI	TCATGA	1 cut(s)	1549
BspLI	GGNNCC	3 cut(s)	62, 330, 1480
BspPI	GGATC	8 cut(s)	152, 323, 336, 679, 1134, 1173, 1473, 1486
BspT104I	TTCGAA	2 cut(s)	15, 1791
BspTNI	GGTCTC	1 cut(s)	1177
BsrDI	GCAATG	1 cut(s)	1785
BsrFI	RCCGGY	2 cut(s)	211, 688
BsrI	ACTGG	1 cut(s)	1303
BssAI	RCCGGY	2 cut(s)	211, 688
BssECI	CCNNGG	3 cut(s)	1473, 1474, 1747
BssHII	GCGCGC	1 cut(s)	1207
BssT1I	CCWWGG	1 cut(s)	1747
Bst2UI	CCWGG	4 cut(s)	65, 1231, 1316, 1475
Bst4CI	ACNGT	5 cut(s)	560, 642, 914, 1219, 1595
Bst6I	CTCTTC	4 cut(s)	219, 292, 499, 778
BstAPI	GCANNNNNTGC	2 cut(s)	700, 1840
BstBAI	YACGTR	1 cut(s)	244
BstBI	TTCGAA	2 cut(s)	15, 1791
BstC8I	GCNNGC	5 cut(s)	213, 690, 927, 1209, 1398
BstDEI	CTNAG	8 cut(s)	6, 187, 413, 988, 1155, 1203, 1223, 1527
BstF5I	GGATG	5 cut(s)	316, 1252, 1490, 1496, 1825
BstFNI	CGCG	2 cut(s)	746, 1209
BstHHI	GCGC	6 cut(s)	206, 694, 746, 1209, 1211, 1533
BstMAI	GTCTC	4 cut(s)	566, 828, 1177, 1671
BstMCI	CGRYCG	2 cut(s)	216, 762
BstNI	CCWGG	4 cut(s)	65, 1231, 1316, 1475
BstNSI	RCATGY	2 cut(s)	1384, 1619
BstSCI	CCNGG	5 cut(s)	57, 63, 1229, 1314, 1473
BstSLI	GKGCMC	1 cut(s)	33
BstUI	CGCG	2 cut(s)	746, 1209
BstV1I	GCAGC	3 cut(s)	706, 983, 1742
BstV2I	GAAGAC	2 cut(s)	454, 1190
BstX2I	RGATCY	4 cut(s)	328, 671, 1139, 1478
BstXI	CCANNNNNNTGG	1 cut(s)	1694
BstYI	RGATCY	4 cut(s)	328, 671, 1139, 1478
BstZI	CGGCCG	2 cut(s)	213, 759
BsuRI	GGCC	6 cut(s)	215, 761, 843, 1134, 1746, 1752
BtgZI	GCGATG	1 cut(s)	732
BtsCI	GGATG	5 cut(s)	316, 1252, 1490, 1496, 1825
BtsI	GCAGTG	1 cut(s)	936
BtsIMutI	CAGTG	3 cut(s)	818, 936, 1038
Cac8I	GCNNGC	5 cut(s)	213, 690, 927, 1209, 1398
CaiI	CAGNNNCTG	1 cut(s)	703
CciI	TCATGA	1 cut(s)	1549
CfoI	GCGC	6 cut(s)	206, 694, 746, 1209, 1211, 1533
Cfr10I	RCCGGY	2 cut(s)	211, 688
Cfr13I	GGNCC	2 cut(s)	61, 638
Csp6I	GTAC	4 cut(s)	652, 849, 901, 1071
CviQI	GTAC	4 cut(s)	652, 849, 901, 1071
DdeI	CTNAG	8 cut(s)	6, 187, 413, 988, 1155, 1203, 1223, 1527
DrdI	GACNNNNNNGTC	1 cut(s)	452
DseDI	GACNNNNNNGTC	1 cut(s)	452
EaeI	YGGCCR	4 cut(s)	213, 759, 841, 1132
EagI	CGGCCG	2 cut(s)	213, 759
Eam1104I	CTCTTC	4 cut(s)	219, 292, 499, 778
EarI	CTCTTC	4 cut(s)	219, 292, 499, 778
EclXI	CGGCCG	2 cut(s)	213, 759
Eco130I	CCWWGG	1 cut(s)	1747
Eco31I	GGTCTC	1 cut(s)	1177
Eco47I	GGWCC	2 cut(s)	61, 638
Eco52I	CGGCCG	2 cut(s)	213, 759
Eco57I	CTGAAG	1 cut(s)	686
Eco72I	CACGTG	1 cut(s)	244
EcoO109I	RGGNCCY	1 cut(s)	61
EcoRII	CCWGG	4 cut(s)	63, 1229, 1314, 1473
EcoT14I	CCWWGG	1 cut(s)	1747
EcoT22I	ATGCAT	1 cut(s)	1617
ErhI	CCWWGG	1 cut(s)	1747
Esp3I	CGTCTC	1 cut(s)	828
FalI	AAGNNNNNCTT	2 cut(s)	1573, 1605
FaqI	GGGAC	5 cut(s)	62, 375, 472, 614, 1692
FauI	CCCGC	2 cut(s)	61, 106
FauNDI	CATATG	1 cut(s)	1611
FbaI	TGATCA	4 cut(s)	246, 1042, 1450, 1552
Fnu4HI	GCNGC	4 cut(s)	695, 841, 972, 1731
FokI	GGATG	5 cut(s)	303, 1239, 1477, 1483, 1832
Fsp4HI	GCNGC	4 cut(s)	695, 841, 972, 1731
FspBI	CTAG	3 cut(s)	675, 1008, 1385
GlaI	GCGC	6 cut(s)	205, 693, 745, 1208, 1210, 1532
GluI	GCNGC	4 cut(s)	695, 841, 972, 1731
GsaI	CCCAGC	4 cut(s)	82, 532, 831, 960
GsuI	CTGGAG	2 cut(s)	548, 725
HaeIII	GGCC	6 cut(s)	215, 761, 843, 1134, 1746, 1752
HapII	CCGG	5 cut(s)	58, 83, 212, 689, 1056
HhaI	GCGC	6 cut(s)	206, 694, 746, 1209, 1211, 1533
Hin6I	GCGC	6 cut(s)	204, 692, 744, 1207, 1209, 1531
HinP1I	GCGC	6 cut(s)	204, 692, 744, 1207, 1209, 1531
HincII	GTYRAC	1 cut(s)	917
HindII	GTYRAC	1 cut(s)	917
HindIII	AAGCTT	1 cut(s)	1407
HinfI	GANTC	6 cut(s)	596, 661, 767, 815, 1268, 1486
HpaII	CCGG	5 cut(s)	58, 83, 212, 689, 1056
HphI	GGTGA	4 cut(s)	96, 818, 1160, 1676
Hpy166II	GTNNAC	2 cut(s)	645, 917
Hpy188I	TCNGA	7 cut(s)	190, 416, 666, 1065, 1156, 1273, 1483
Hpy8I	GTNNAC	2 cut(s)	645, 917
Hpy99I	CGWCG	3 cut(s)	461, 815, 887
HpyAV	CCTTC	4 cut(s)	39, 661, 1470, 1809
HpyCH4III	ACNGT	5 cut(s)	560, 642, 914, 1219, 1595
HpyCH4IV	ACGT	2 cut(s)	243, 1738
HpyCH4V	TGCA	9 cut(s)	925, 971, 983, 1252, 1340, 1615, 1730, 1778, 1843
HpyF3I	CTNAG	8 cut(s)	6, 187, 413, 988, 1155, 1203, 1223, 1527
HpySE526I	ACGT	2 cut(s)	243, 1738
HspAI	GCGC	6 cut(s)	204, 692, 744, 1207, 1209, 1531
Kpn2I	TCCGGA	1 cut(s)	1055
KroI	GCCGGC	2 cut(s)	211, 688
KroNI	GCCGGC	2 cut(s)	213, 690
Ksp22I	TGATCA	4 cut(s)	246, 1042, 1450, 1552
LmnI	GCTCC	5 cut(s)	60, 147, 979, 1053, 1717
Lsp1109I	GCAGC	3 cut(s)	706, 983, 1742
LweI	GCATC	4 cut(s)	110, 912, 1261, 1532
MaeI	CTAG	3 cut(s)	675, 1008, 1385
MaeII	ACGT	2 cut(s)	243, 1738
MaeIII	GTNAC	2 cut(s)	806, 1213
MboII	GAAGA	9 cut(s)	236, 279, 454, 486, 489, 765, 1039, 1190, 1382
MflI	RGATCY	4 cut(s)	328, 671, 1139, 1478
MhlI	GDGCHC	1 cut(s)	33
MlsI	TGGCCA	1 cut(s)	1134
MluCI	AATT	9 cut(s)	274, 335, 589, 604, 735, 870, 1277, 1324, 1597
MluNI	TGGCCA	1 cut(s)	1134
MlyI	GAGTC	2 cut(s)	809, 1262
MmeI	TCCRAC	1 cut(s)	118
Mox20I	TGGCCA	1 cut(s)	1134
Mph1103I	ATGCAT	1 cut(s)	1617
MroI	TCCGGA	1 cut(s)	1055
MroNI	GCCGGC	2 cut(s)	211, 688
MscI	TGGCCA	1 cut(s)	1134
MseI	TTAA	6 cut(s)	588, 1017, 1079, 1280, 1335, 1427
MslI	CAYNNNNRTG	1 cut(s)	1614
Msp20I	TGGCCA	1 cut(s)	1134
MspA1I	CMGCKG	1 cut(s)	1733
MspI	CCGG	5 cut(s)	58, 83, 212, 689, 1056
MspR9I	CCNGG	5 cut(s)	59, 65, 1231, 1316, 1475
Mva1269I	GAATGC	1 cut(s)	1036
MvaI	CCWGG	4 cut(s)	65, 1231, 1316, 1475
MvnI	CGCG	2 cut(s)	746, 1209
NaeI	GCCGGC	2 cut(s)	213, 690
NciI	CCSGG	1 cut(s)	59
NdeI	CATATG	1 cut(s)	1611
NgoMIV	GCCGGC	2 cut(s)	211, 688
NlaIV	GGNNCC	3 cut(s)	62, 330, 1480
NmeAIII	GCCGAG	1 cut(s)	232
NmuCI	GTSAC	1 cut(s)	806
NsiI	ATGCAT	1 cut(s)	1617
NspI	RCATGY	2 cut(s)	1384, 1619
NspV	TTCGAA	2 cut(s)	15, 1791
PagI	TCATGA	1 cut(s)	1549
PasI	CCCWGGG	1 cut(s)	1474
PauI	GCGCGC	1 cut(s)	1207
PcsI	WCGNNNNNNNCGW	2 cut(s)	453, 1764
PctI	GAATGC	1 cut(s)	1036
PdiI	GCCGGC	2 cut(s)	213, 690
PfeI	GAWTC	4 cut(s)	596, 661, 767, 1486
PflMI	CCANNNNNTGG	1 cut(s)	259
PkrI	GCNGC	4 cut(s)	696, 842, 973, 1732
PleI	GAGTC	2 cut(s)	809, 1262
PmaCI	CACGTG	1 cut(s)	244
PmlI	CACGTG	1 cut(s)	244
PpsI	GAGTC	2 cut(s)	809, 1262
Ppu21I	YACGTR	1 cut(s)	244
PpuMI	RGGWCCY	1 cut(s)	61
PshBI	ATTAAT	3 cut(s)	588, 1280, 1335
Psp1406I	AACGTT	1 cut(s)	1738
Psp5II	RGGWCCY	1 cut(s)	61
Psp6I	CCWGG	4 cut(s)	63, 1229, 1314, 1473
PspCI	CACGTG	1 cut(s)	244
PspFI	CCCAGC	4 cut(s)	78, 528, 827, 956
PspGI	CCWGG	4 cut(s)	63, 1229, 1314, 1473
PspN4I	GGNNCC	3 cut(s)	62, 330, 1480
PspPI	GGNCC	2 cut(s)	61, 638
PspPPI	RGGWCCY	1 cut(s)	61
PstNI	CAGNNNCTG	1 cut(s)	703
PsuI	RGATCY	4 cut(s)	328, 671, 1139, 1478
PteI	GCGCGC	1 cut(s)	1207
PvuII	CAGCTG	1 cut(s)	1733
RsaI	GTAC	4 cut(s)	653, 850, 902, 1072
RsaNI	GTAC	4 cut(s)	652, 849, 901, 1071
RseI	CAYNNNNRTG	1 cut(s)	1614
SaqAI	TTAA	6 cut(s)	588, 1017, 1079, 1280, 1335, 1427
SatI	GCNGC	4 cut(s)	695, 841, 972, 1731
Sau96I	GGNCC	2 cut(s)	61, 638
SchI	GAGTC	2 cut(s)	809, 1262
ScrFI	CCNGG	5 cut(s)	59, 65, 1231, 1316, 1475
SduI	GDGCHC	1 cut(s)	33
SfaNI	GCATC	4 cut(s)	110, 912, 1261, 1532
SfuI	TTCGAA	2 cut(s)	15, 1791
SinI	GGWCC	2 cut(s)	61, 638
SmiMI	CAYNNNNRTG	1 cut(s)	1614
SmlI	CTYRAG	1 cut(s)	396
SmoI	CTYRAG	1 cut(s)	396
Sse9I	AATT	9 cut(s)	274, 335, 589, 604, 735, 870, 1277, 1324, 1597
SsiI	CCGC	5 cut(s)	68, 99, 354, 840, 1521
SspMI	CTAG	3 cut(s)	675, 1008, 1385
StyD4I	CCNGG	5 cut(s)	57, 63, 1229, 1314, 1473
StyI	CCWWGG	1 cut(s)	1747
TaaI	ACNGT	5 cut(s)	560, 642, 914, 1219, 1595
TaiI	ACGT	2 cut(s)	246, 1741
TaqI	TCGA	5 cut(s)	15, 423, 659, 882, 1791
TasI	AATT	9 cut(s)	274, 335, 589, 604, 735, 870, 1277, 1324, 1597
TatI	WGTACW	1 cut(s)	848
TauI	GCSGC	1 cut(s)	843
TfiI	GAWTC	4 cut(s)	596, 661, 767, 1486
Tru1I	TTAA	6 cut(s)	588, 1017, 1079, 1280, 1335, 1427
Tru9I	TTAA	6 cut(s)	588, 1017, 1079, 1280, 1335, 1427
TscAI	CASTG	3 cut(s)	825, 936, 1045
TseFI	GTSAC	1 cut(s)	806
TseI	GCWGC	3 cut(s)	694, 971, 1730
Tsp45I	GTSAC	1 cut(s)	806
TspDTI	ATGAA	7 cut(s)	163, 1100, 1101, 1110, 1136, 1538, 1649
TspRI	CASTG	3 cut(s)	825, 936, 1045
Van91I	CCANNNNNTGG	1 cut(s)	259
VpaK11BI	GGWCC	2 cut(s)	61, 638
VspI	ATTAAT	3 cut(s)	588, 1280, 1335
XapI	RAATTY	2 cut(s)	604, 735
XceI	RCATGY	2 cut(s)	1384, 1619
XspI	CTAG	3 cut(s)	675, 1008, 1385
Zsp2I	ATGCAT	1 cut(s)	1617

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.