Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0019283

Physical Location & Seq

Genomic Coordinates Forward (+)

25164169 .. 25166268

Gene Structure 2100 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1170600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 705 bp

ATGACAGTTCCGCCGTATCAGGATGATGTTAAGAATAATGAGAGGACGAATTACTTCAATTCCCCTGCATTGGATGTCTCAGTTGTTTTCCGCAAGCAACGCCGGCTTCTACTTTTCCTCCCTGCATCATTGCTCGTGCACCTCTTTAGTTCTTCGGATTATTACAAGATTGATGATCTATTGACACCTGAGGAGCAGGCTATTAGGTTGAGTGTAAGAGAGTGTATGGAGAAAGACGTTGCTCCAATTATGGCAGAGTATTGGGAGAAGGCAGAGTTTCCATTTCAAATTATTCCAAAGCTTGGTGCATTGCGCATTGCTGGTGGGACAATCAAGGGTTATGGGTGTCCTGGTCTTTCTATCACGCCAAATGCTTTTGCTACAGCTGAAATTGCTAGAGTTGATGCAAGCTGTTCTACTTTTTTCTTGGTTCATTCCTCACTAGCAATGCTTACTATTGCATTATGTGGGTCGGAGGCTCAAAAGCAGAAATATCTACCTTCTTTGGCTGAATTTAAGACTGTCGCCTGCTGGGGTTTGACTGAGCCTGACTATGGAAGTGATGCGAGTGCCTTGAGAGCGACAGCAACAAAGGTGGAAGGAGGTTGGGTACTTGAAGGCCAAAAGCGATGGATAGGAAACAGTACATTTGCTGATATCTTGATTATTTTTGCTCGGAATACAATCAGATTAATGGGTATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0003997 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006082 GO:0006629 GO:0006631 GO:0006635 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009514 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0016042 GO:0016054 GO:0016491 GO:0016627 GO:0016634 GO:0019395 GO:0019752 GO:0022414 GO:0030258 GO:0032501 GO:0032502 GO:0032787 GO:0034440 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0046395 GO:0046459 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0055114 GO:0061458 GO:0071704 GO:0072329 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00071 ko00592 ko01040 ko01100 ko01110 ko01212 ko03320 ko04024 ko04146 map00071 map00592 map01040 map01100 map01110 map01212 map03320 map04024 map04146

Pfam Domains

Protein Families

Acyl-CoA_dh_1 Acyl-CoA_dh_M Acyl-CoA_dh_N

Protein Analysis

235

Amino Acids

25.97

Weight (kDa)

6.17

Isoelectric Point (pI)

44.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Acyl-CoA_dh_N	PF02771	62 - 171	9e-28	Acyl-CoA dehydrogenase, N-terminal domain
Acyl-CoA_dh_M	PF02770	177 - 226	3.2e-12	Acyl-CoA dehydrogenase, middle domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0019283)

Species	Orthologous Gene IDs
malus_domestica	MD12G1169800.v1.1 MD12G1170600.v1.1
pyrus_communis	pycom04g14040 pycom12g10520 pycom12g16040 pycom12g16090 pycom16g21080
rosa_multiflora	Rmu_sc0011275.1_g000001

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	314
AccB7I	CCANNNNNTGG	1 cut(s)	302
AciI	CCGC	2 cut(s)	11, 91
AcsI	RAATTY	1 cut(s)	512
AfaI	GTAC	2 cut(s)	612, 646
AfiI	CCNNNNNNNGG	3 cut(s)	70, 302, 554
AgsI	TTSAA	3 cut(s)	58, 287, 617
AjnI	CCWGG	1 cut(s)	349
AluBI	AGCT	3 cut(s)	301, 386, 411
AluI	AGCT	3 cut(s)	301, 386, 411
Alw21I	GWGCWC	1 cut(s)	141
Alw26I	GTCTC	1 cut(s)	82
Alw44I	GTGCAC	1 cut(s)	137
AoxI	GGCC	1 cut(s)	619
ApaLI	GTGCAC	1 cut(s)	137
ApoI	RAATTY	1 cut(s)	512
AseI	ATTAAT	1 cut(s)	692
Asp700I	GAANNNNTTC	1 cut(s)	53
AspLEI	GCGC	1 cut(s)	315
AxyI	CCTNAGG	1 cut(s)	189
BaeGI	GKGCMC	1 cut(s)	141
BauI	CACGAG	1 cut(s)	134
Bbv12I	GWGCWC	1 cut(s)	141
BccI	CCATC	1 cut(s)	624
BciT130I	CCWGG	1 cut(s)	351
BcoDI	GTCTC	1 cut(s)	82
BfaI	CTAG	2 cut(s)	396, 443
BfmI	CTRYAG	1 cut(s)	381
Bme1390I	CCNGG	1 cut(s)	351
BmrFI	CCNGG	1 cut(s)	351
BmsI	GCATC	3 cut(s)	134, 394, 553
BpuEI	CTTGAG	1 cut(s)	595
BsaXI	ACNNNNNCTCC	4 cut(s)	102, 132, 594, 624
Bsc4I	CCNNNNNNNGG	3 cut(s)	70, 302, 554
Bse118I	RCCGGY	1 cut(s)	102
Bse21I	CCTNAGG	1 cut(s)	189
Bse3DI	GCAATG	4 cut(s)	128, 308, 315, 453
BseBI	CCWGG	1 cut(s)	351
BseGI	GGATG	2 cut(s)	28, 79
BseLI	CCNNNNNNNGG	3 cut(s)	70, 302, 554
BseMI	GCAATG	4 cut(s)	128, 308, 315, 453
BseMII	CTCAG	3 cut(s)	93, 180, 534
BseRI	GAGGAG	1 cut(s)	206
BseSI	GKGCMC	1 cut(s)	141
BseYI	CCCAGC	1 cut(s)	531
BshFI	GGCC	1 cut(s)	621
BsiHKAI	GWGCWC	1 cut(s)	141
BsiSI	CCGG	1 cut(s)	103
BslFI	GGGAC	1 cut(s)	340
BslI	CCNNNNNNNGG	3 cut(s)	70, 302, 554
BsmAI	GTCTC	1 cut(s)	82
BsmFI	GGGAC	1 cut(s)	340
BsnI	GGCC	1 cut(s)	621
Bsp1286I	GDGCHC	1 cut(s)	141
Bsp143I	GATC	1 cut(s)	175
BspACI	CCGC	2 cut(s)	11, 91
BspANI	GGCC	1 cut(s)	621
BspCNI	CTCAG	3 cut(s)	92, 181, 535
BsrDI	GCAATG	4 cut(s)	128, 308, 315, 453
BsrFI	RCCGGY	1 cut(s)	102
BssAI	RCCGGY	1 cut(s)	102
BssMI	GATC	1 cut(s)	175
BssSI	CACGAG	1 cut(s)	134
Bst2BI	CACGAG	1 cut(s)	134
Bst2UI	CCWGG	1 cut(s)	351
Bst4CI	ACNGT	3 cut(s)	7, 523, 644
BstC8I	GCNNGC	5 cut(s)	95, 104, 198, 409, 529
BstDEI	CTNAG	3 cut(s)	79, 189, 543
BstF5I	GGATG	2 cut(s)	28, 79
BstHHI	GCGC	1 cut(s)	315
BstKTI	GATC	1 cut(s)	178
BstMAI	GTCTC	1 cut(s)	82
BstMBI	GATC	1 cut(s)	175
BstMWI	GCNNNNNNNGC	4 cut(s)	99, 103, 392, 578
BstNI	CCWGG	1 cut(s)	351
BstSCI	CCNGG	1 cut(s)	349
BstSFI	CTRYAG	1 cut(s)	381
BstSLI	GKGCMC	1 cut(s)	141
Bsu36I	CCTNAGG	1 cut(s)	189
BsuRI	GGCC	1 cut(s)	621
BtgZI	GCGATG	1 cut(s)	643
BtsCI	GGATG	2 cut(s)	28, 79
Cac8I	GCNNGC	5 cut(s)	95, 104, 198, 409, 529
CfoI	GCGC	1 cut(s)	315
Cfr10I	RCCGGY	1 cut(s)	102
Csp6I	GTAC	2 cut(s)	611, 645
CspCI	CAANNNNNGTGG	2 cut(s)	576, 611
CviJI	RGCY	9 cut(s)	106, 200, 301, 386, 411, 479, 509, 547, 621
CviKI_1	RGCY	9 cut(s)	106, 200, 301, 386, 411, 479, 509, 547, 621
CviQI	GTAC	2 cut(s)	611, 645
DdeI	CTNAG	3 cut(s)	79, 189, 543
DpnI	GATC	1 cut(s)	177
DpnII	GATC	1 cut(s)	175
Eco32I	GATATC	1 cut(s)	658
Eco81I	CCTNAGG	1 cut(s)	189
EcoRII	CCWGG	1 cut(s)	349
EcoRV	GATATC	1 cut(s)	658
FaiI	YATR	6 cut(s)	227, 251, 342, 466, 555, 701
FaqI	GGGAC	1 cut(s)	340
FokI	GGATG	2 cut(s)	35, 86
FspBI	CTAG	2 cut(s)	396, 443
FspI	TGCGCA	1 cut(s)	314
GlaI	GCGC	1 cut(s)	314
GsaI	CCCAGC	1 cut(s)	535
HaeIII	GGCC	1 cut(s)	621
HapII	CCGG	1 cut(s)	103
HhaI	GCGC	1 cut(s)	315
Hin6I	GCGC	1 cut(s)	313
HinP1I	GCGC	1 cut(s)	313
HindIII	AAGCTT	1 cut(s)	299
HpaII	CCGG	1 cut(s)	103
Hpy166II	GTNNAC	1 cut(s)	139
Hpy188I	TCNGA	4 cut(s)	157, 475, 678, 689
Hpy188III	TCNNGA	2 cut(s)	20, 661
Hpy8I	GTNNAC	1 cut(s)	139
HpyAV	CCTTC	4 cut(s)	262, 510, 593, 611
HpyCH4III	ACNGT	3 cut(s)	7, 523, 644
HpyCH4IV	ACGT	1 cut(s)	237
HpyCH4V	TGCA	6 cut(s)	68, 125, 139, 308, 407, 461
HpyF10VI	GCNNNNNNNGC	4 cut(s)	99, 103, 392, 578
HpyF3I	CTNAG	3 cut(s)	79, 189, 543
HpySE526I	ACGT	1 cut(s)	237
HspAI	GCGC	1 cut(s)	313
KroI	GCCGGC	1 cut(s)	102
KroNI	GCCGGC	1 cut(s)	104
Kzo9I	GATC	1 cut(s)	175
LmnI	GCTCC	2 cut(s)	193, 247
LweI	GCATC	3 cut(s)	134, 394, 553
MaeI	CTAG	2 cut(s)	396, 443
MaeII	ACGT	1 cut(s)	237
MalI	GATC	1 cut(s)	177
MboI	GATC	1 cut(s)	175
MboII	GAAGA	1 cut(s)	144
MhlI	GDGCHC	1 cut(s)	141
MluCI	AATT	6 cut(s)	49, 58, 246, 288, 390, 512
MmeI	TCCRAC	1 cut(s)	453
MnlI	CCTC	7 cut(s)	36, 128, 152, 184, 448, 469, 596
MroNI	GCCGGC	1 cut(s)	102
MroXI	GAANNNNTTC	1 cut(s)	53
MseI	TTAA	3 cut(s)	30, 516, 692
MspA1I	CMGCKG	1 cut(s)	386
MspI	CCGG	1 cut(s)	103
MspR9I	CCNGG	1 cut(s)	351
MvaI	CCWGG	1 cut(s)	351
MwoI	GCNNNNNNNGC	4 cut(s)	99, 103, 392, 578
NaeI	GCCGGC	1 cut(s)	104
NdeII	GATC	1 cut(s)	175
NgoMIV	GCCGGC	1 cut(s)	102
NsbI	TGCGCA	1 cut(s)	314
PdiI	GCCGGC	1 cut(s)	104
PdmI	GAANNNNTTC	1 cut(s)	53
PflMI	CCANNNNNTGG	1 cut(s)	302
PshBI	ATTAAT	1 cut(s)	692
Psp6I	CCWGG	1 cut(s)	349
PspFI	CCCAGC	1 cut(s)	531
PspGI	CCWGG	1 cut(s)	349
PvuII	CAGCTG	1 cut(s)	386
RsaI	GTAC	2 cut(s)	612, 646
RsaNI	GTAC	2 cut(s)	611, 645
SaqAI	TTAA	3 cut(s)	30, 516, 692
Sau3AI	GATC	1 cut(s)	175
ScrFI	CCNGG	1 cut(s)	351
SduI	GDGCHC	1 cut(s)	141
SfaNI	GCATC	3 cut(s)	134, 394, 553
SfcI	CTRYAG	1 cut(s)	381
SmlI	CTYRAG	1 cut(s)	574
SmoI	CTYRAG	1 cut(s)	574
Sse9I	AATT	6 cut(s)	49, 58, 246, 288, 390, 512
SsiI	CCGC	2 cut(s)	11, 91
SspMI	CTAG	2 cut(s)	396, 443
StyD4I	CCNGG	1 cut(s)	349
TaaI	ACNGT	3 cut(s)	7, 523, 644
TaiI	ACGT	1 cut(s)	240
TasI	AATT	6 cut(s)	49, 58, 246, 288, 390, 512
TatI	WGTACW	1 cut(s)	644
Tru1I	TTAA	3 cut(s)	30, 516, 692
Tru9I	TTAA	3 cut(s)	30, 516, 692
TspDTI	ATGAA	1 cut(s)	422
Van91I	CCANNNNNTGG	1 cut(s)	302
VneI	GTGCAC	1 cut(s)	137
VspI	ATTAAT	1 cut(s)	692
XapI	RAATTY	1 cut(s)	512
XmnI	GAANNNNTTC	1 cut(s)	53
XspI	CTAG	2 cut(s)	396, 443

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.