Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0012809

Physical Location & Seq

Genomic Coordinates Forward (+)

25244617 .. 25246841

Gene Structure 2225 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1172100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 978 bp

ATGGAAGAAAACCGAAATGAAAACCCCAATCCGACGAGCCCACCACAGCCGCCGCCGCCGCCGCCCAAGAAGCCGCCGATTCCGCCGTACGTGAAGGCCCTGTCGGGTTCGCTCGGCGGCATCATGGAGGCCGCGTGCCTACAGCCCATCGATGTCATCAAGACCCGGCTCCAGCTCGACCGGACCGGCACCTACAAGGGAATCATCCACTGCGGCGCCACCGTTGCCCGCACCGAGGGAGTCCGGGCTCTCTGGAAGGGCCTGACCCCCTTCGCCACCCACCTGACCTTGAAGTACGCGCTTCGGATGGGCTCCAACGCCGTGCTGCAGGGCGCGTTTAAGGACGCGGAGACCGGGAAGGTCAGCAACCATGGCCGGCTCATTTCTGGGTTCGGAGCTGGAGTTCTCGAAGCTCTCGTCATTGTCACGCCGTTTGAGGTGGTGAAAATTAGACTACAGCAGCAGAAAGGATTGAGTCTTGGGCTTCTGAAGTATAAGGGTCCTATTCACTGTGCTCGTACGATCATTCGGGAGGAAGGTATCCGTGGGCTTTGGTCAGGTGCTGCTCCGACTGTTATGCGCAATGGCACGAACCAGGCTGCCATGTTTACAGCCAAAAATGCGTTCGATGTTCTCTTGTGGAAGAAACATGAAGGAGATGGGAGAGTCCTGCTGCCATGGCAATCTATGATATCTGGTTTCCTTGCAGGCACAGCCGGCCCCATTTGCACGGGTCCCTTTGATGTTGTGAAAACGAGGCTGATGGCTCAGAGCCGAGGTGTGGATGGCAAGATGAAATACAAGGGCATGATTCATGCGATCAGGACAATATACGCAGAGGAAGGGCTTCTGGCTTTGTGGAAAGGACTCCTGCCTCGGCTCATGAGGATACCACCCGGGCAAGCCATTATGTGGACTGTGGCTGACCAAGTGATAGGATTGTATGAGAAACGATATCTTCGTAATGCACCCTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005310 GO:0005342 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0006810 GO:0006811 GO:0006820 GO:0006835 GO:0006839 GO:0006855 GO:0008150 GO:0008509 GO:0008514 GO:0015075 GO:0015141 GO:0015238 GO:0015318 GO:0015556 GO:0015711 GO:0015740 GO:0015741 GO:0015744 GO:0015849 GO:0015893 GO:0016020 GO:0016021 GO:0019866 GO:0022857 GO:0031090 GO:0031224 GO:0031966 GO:0031967 GO:0031975 GO:0034220 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046942 GO:0046943 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0071422 GO:0071702 GO:0098656 GO:1903825 GO:1905039

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Mito_carr

Protein Analysis

326

Amino Acids

35.43

Weight (kDa)

10.01

Isoelectric Point (pI)

35.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Mito_carr	PF00153	28 - 113	2.4e-17	Mitochondrial carrier protein
Mito_carr	PF00153	122 - 216	1.2e-20	Mitochondrial carrier protein
Mito_carr	PF00153	226 - 316	9.5e-24	Mitochondrial carrier protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012809)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01340
fragaria_vesca	FvH4_6g11130
malus_domestica	MD00G1062100.v1.1 MD12G1172100.v1.1
prunus_persica	Prupe.6G280400_v2.0.a1
pyrus_communis	pycom04g14300 pycom12g16330
rosa_chinensis	RchiOBHm_Chr3g0462571
rosa_laevigata	RLG00000024847
rosa_multiflora	Rmu_sc0002882.1_g000001 Rmu_sc0003624.1_g000023
rosa_roxburghii	Rroxscaffold_6G00417380
rosa_rugosa	Rorug03G0057600
rosa_samantha	Rh3AG115700 Rh3BG119200 Rh3CG122600 Rh3DG121300
rosa_wichuraiana	Rw3G009810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	581
AccB1I	GGYRCC	2 cut(s)	188, 215
AccB7I	CCANNNNNTGG	1 cut(s)	912
AccII	CGCG	4 cut(s)	134, 299, 335, 347
AcoI	YGGCCR	1 cut(s)	373
AcuI	CTGAAG	1 cut(s)	509
AcyI	GRCGYC	1 cut(s)	216
AfaI	GTAC	3 cut(s)	89, 296, 520
AfiI	CCNNNNNNNGG	3 cut(s)	235, 781, 912
AgsI	TTSAA	1 cut(s)	292
AjnI	CCWGG	1 cut(s)	594
AjuI	GAANNNNNNNTTGG	2 cut(s)	608, 640
AloI	GAACNNNNNNTCC	2 cut(s)	387, 419
AluBI	AGCT	3 cut(s)	175, 398, 413
AluI	AGCT	3 cut(s)	175, 398, 413
Alw21I	GWGCWC	1 cut(s)	517
Alw26I	GTCTC	1 cut(s)	344
AlwNI	CAGNNNCTG	1 cut(s)	563
Ama87I	CYCGRG	1 cut(s)	896
AoxI	GGCC	5 cut(s)	96, 129, 259, 373, 718
ApeKI	GCWGC	5 cut(s)	325, 460, 563, 599, 673
ArsI	GACNNNNNNTTYG	2 cut(s)	402, 434
Asp700I	GAANNNNTTC	1 cut(s)	846
AspLEI	GCGC	4 cut(s)	218, 301, 335, 582
AspS9I	GGNCC	6 cut(s)	97, 183, 259, 500, 719, 734
AsuC2I	CCSGG	5 cut(s)	166, 245, 355, 897, 898
AsuHPI	GGTGA	1 cut(s)	454
AvaI	CYCGRG	1 cut(s)	896
AvaII	GGWCC	3 cut(s)	183, 500, 734
BanI	GGYRCC	2 cut(s)	188, 215
BanII	GRGCYC	3 cut(s)	41, 250, 314
Bbv12I	GWGCWC	1 cut(s)	517
BbvI	GCAGC	5 cut(s)	312, 472, 550, 586, 660
BccI	CCATC	5 cut(s)	155, 301, 653, 757, 779
BceAI	ACGGC	3 cut(s)	70, 305, 415
BcgI	CGANNNNNNTGC	2 cut(s)	559, 593
BciT130I	CCWGG	1 cut(s)	596
BciVI	GTATCC	2 cut(s)	551, 882
BcnI	CCSGG	5 cut(s)	166, 245, 355, 897, 898
BcoDI	GTCTC	1 cut(s)	344
BfmI	CTRYAG	3 cut(s)	140, 326, 455
BfoI	RGCGCY	1 cut(s)	219
BfuI	GTATCC	2 cut(s)	551, 882
Bme1390I	CCNGG	6 cut(s)	166, 245, 355, 596, 897, 898
Bme18I	GGWCC	3 cut(s)	183, 500, 734
BmeT110I	CYCGRG	1 cut(s)	896
BmgT120I	GGNCC	6 cut(s)	97, 183, 259, 500, 719, 734
BmiI	GGNNCC	8 cut(s)	170, 190, 217, 313, 501, 721, 735, 736
BmrFI	CCNGG	6 cut(s)	166, 245, 355, 596, 897, 898
BmsI	GCATC	1 cut(s)	129
BpmI	CTGGAG	2 cut(s)	155, 420
BpuMI	CCSGG	5 cut(s)	166, 245, 355, 897, 898
Bsa29I	ATCGAT	1 cut(s)	150
BsaAI	YACGTR	1 cut(s)	91
BsaHI	GRCGYC	1 cut(s)	216
BsaI	GGTCTC	1 cut(s)	344
BsaJI	CCNNGG	7 cut(s)	234, 370, 544, 677, 775, 875, 896
BsaWI	WCCGGW	1 cut(s)	180
BsaXI	ACNNNNNCTCC	4 cut(s)	119, 149, 387, 417
Bsc4I	CCNNNNNNNGG	3 cut(s)	235, 781, 912
Bse118I	RCCGGY	3 cut(s)	185, 375, 716
Bse3DI	GCAATG	1 cut(s)	589
BseBI	CCWGG	1 cut(s)	596
BseCI	ATCGAT	1 cut(s)	150
BseDI	CCNNGG	7 cut(s)	234, 370, 544, 677, 775, 875, 896
BseGI	GGATG	3 cut(s)	204, 312, 790
BseLI	CCNNNNNNNGG	3 cut(s)	235, 781, 912
BseMI	GCAATG	1 cut(s)	589
BseMII	CTCAG	1 cut(s)	782
BseXI	GCAGC	5 cut(s)	312, 472, 550, 586, 660
Bsh1236I	CGCG	4 cut(s)	134, 299, 335, 347
Bsh1285I	CGRYCG	1 cut(s)	181
BshFI	GGCC	5 cut(s)	98, 131, 261, 375, 720
BshNI	GGYRCC	2 cut(s)	188, 215
BshVI	ATCGAT	1 cut(s)	150
BsiEI	CGRYCG	1 cut(s)	181
BsiHKAI	GWGCWC	1 cut(s)	517
BsiHKCI	CYCGRG	1 cut(s)	896
BsiSI	CCGG	8 cut(s)	166, 181, 186, 244, 354, 376, 717, 897
BsiWI	CGTACG	2 cut(s)	87, 518
BslFI	GGGAC	1 cut(s)	720
BslI	CCNNNNNNNGG	3 cut(s)	235, 781, 912
BsmAI	GTCTC	1 cut(s)	344
BsmFI	GGGAC	1 cut(s)	720
BsnI	GGCC	5 cut(s)	98, 131, 261, 375, 720
Bso31I	GGTCTC	1 cut(s)	344
BsoBI	CYCGRG	1 cut(s)	896
Bsp1286I	GDGCHC	4 cut(s)	41, 250, 314, 517
Bsp143I	GATC	2 cut(s)	522, 819
Bsp19I	CCATGG	2 cut(s)	370, 677
BspANI	GGCC	5 cut(s)	98, 131, 261, 375, 720
BspCNI	CTCAG	1 cut(s)	781
BspDI	ATCGAT	1 cut(s)	150
BspFNI	CGCG	4 cut(s)	134, 299, 335, 347
BspHI	TCATGA	1 cut(s)	882
BspLI	GGNNCC	8 cut(s)	170, 190, 217, 313, 501, 721, 735, 736
BspMAI	CTGCAG	1 cut(s)	330
BspT107I	GGYRCC	2 cut(s)	188, 215
BspTNI	GGTCTC	1 cut(s)	344
BsrDI	GCAATG	1 cut(s)	589
BsrFI	RCCGGY	3 cut(s)	185, 375, 716
BssAI	RCCGGY	3 cut(s)	185, 375, 716
BssECI	CCNNGG	7 cut(s)	234, 370, 544, 677, 775, 875, 896
BssMI	GATC	2 cut(s)	522, 819
BssNI	GRCGYC	1 cut(s)	216
BssT1I	CCWWGG	2 cut(s)	370, 677
Bst2UI	CCWGG	1 cut(s)	596
Bst4CI	ACNGT	4 cut(s)	223, 512, 574, 919
BstACI	GRCGYC	1 cut(s)	216
BstBAI	YACGTR	1 cut(s)	91
BstC8I	GCNNGC	6 cut(s)	136, 229, 377, 709, 718, 903
BstDEI	CTNAG	1 cut(s)	768
BstDSI	CCRYGG	3 cut(s)	370, 544, 677
BstF5I	GGATG	3 cut(s)	204, 312, 790
BstFNI	CGCG	4 cut(s)	134, 299, 335, 347
BstH2I	RGCGCY	1 cut(s)	219
BstHHI	GCGC	4 cut(s)	218, 301, 335, 582
BstKTI	GATC	2 cut(s)	525, 822
BstMAI	GTCTC	1 cut(s)	344
BstMBI	GATC	2 cut(s)	522, 819
BstMCI	CGRYCG	1 cut(s)	181
BstNI	CCWGG	1 cut(s)	596
BstSCI	CCNGG	6 cut(s)	164, 243, 353, 594, 895, 896
BstSFI	CTRYAG	3 cut(s)	140, 326, 455
BstUI	CGCG	4 cut(s)	134, 299, 335, 347
BstV1I	GCAGC	5 cut(s)	312, 472, 550, 586, 660
Bsu15I	ATCGAT	1 cut(s)	150
BsuI	GTATCC	2 cut(s)	551, 882
BsuRI	GGCC	5 cut(s)	98, 131, 261, 375, 720
BsuTUI	ATCGAT	1 cut(s)	150
BtgI	CCRYGG	3 cut(s)	370, 544, 677
BtsCI	GGATG	3 cut(s)	204, 312, 790
BtsI	GCAGTG	1 cut(s)	208
BtsIMutI	CAGTG	2 cut(s)	208, 508
Cac8I	GCNNGC	6 cut(s)	136, 229, 377, 709, 718, 903
CaiI	CAGNNNCTG	1 cut(s)	563
CciI	TCATGA	1 cut(s)	882
CfoI	GCGC	4 cut(s)	218, 301, 335, 582
Cfr10I	RCCGGY	3 cut(s)	185, 375, 716
Cfr13I	GGNCC	6 cut(s)	97, 183, 259, 500, 719, 734
Cfr9I	CCCGGG	1 cut(s)	896
ClaI	ATCGAT	1 cut(s)	150
CpoI	CGGWCCG	1 cut(s)	183
CseI	GACGC	1 cut(s)	353
Csp6I	GTAC	3 cut(s)	88, 295, 519
CspI	CGGWCCG	1 cut(s)	183
CviAII	CATG	8 cut(s)	124, 371, 604, 650, 678, 808, 815, 883
CviQI	GTAC	3 cut(s)	88, 295, 519
DdeI	CTNAG	1 cut(s)	768
DinI	GGCGCC	1 cut(s)	217
DpnI	GATC	2 cut(s)	524, 821
DpnII	GATC	2 cut(s)	522, 819
EaeI	YGGCCR	1 cut(s)	373
EciI	GGCGGA	1 cut(s)	72
Eco130I	CCWWGG	2 cut(s)	370, 677
Eco24I	GRGCYC	3 cut(s)	41, 250, 314
Eco31I	GGTCTC	1 cut(s)	344
Eco32I	GATATC	2 cut(s)	693, 956
Eco47I	GGWCC	3 cut(s)	183, 500, 734
Eco57I	CTGAAG	1 cut(s)	509
Eco88I	CYCGRG	1 cut(s)	896
EcoO109I	RGGNCCY	4 cut(s)	97, 259, 500, 734
EcoRII	CCWGG	1 cut(s)	594
EcoRV	GATATC	2 cut(s)	693, 956
EcoT14I	CCWWGG	2 cut(s)	370, 677
EcoT38I	GRGCYC	3 cut(s)	41, 250, 314
EgeI	GGCGCC	1 cut(s)	217
EheI	GGCGCC	1 cut(s)	217
ErhI	CCWWGG	2 cut(s)	370, 677
FaeI	CATG	8 cut(s)	127, 374, 607, 653, 681, 811, 818, 886
FaqI	GGGAC	1 cut(s)	720
FatI	CATG	8 cut(s)	123, 370, 603, 649, 677, 807, 814, 882
FauI	CCCGC	1 cut(s)	236
FokI	GGATG	3 cut(s)	191, 319, 797
FriOI	GRGCYC	3 cut(s)	41, 250, 314
FspI	TGCGCA	1 cut(s)	581
GlaI	GCGC	4 cut(s)	217, 300, 334, 581
GsuI	CTGGAG	2 cut(s)	155, 420
HaeII	RGCGCY	1 cut(s)	219
HaeIII	GGCC	5 cut(s)	98, 131, 261, 375, 720
HapII	CCGG	8 cut(s)	166, 181, 186, 244, 354, 376, 717, 897
HgaI	GACGC	1 cut(s)	353
HhaI	GCGC	4 cut(s)	218, 301, 335, 582
Hin1I	GRCGYC	1 cut(s)	216
Hin1II	CATG	8 cut(s)	127, 374, 607, 653, 681, 811, 818, 886
Hin6I	GCGC	4 cut(s)	216, 299, 333, 580
HinP1I	GCGC	4 cut(s)	216, 299, 333, 580
HinfI	GANTC	7 cut(s)	79, 201, 240, 475, 666, 811, 867
HpaII	CCGG	8 cut(s)	166, 181, 186, 244, 354, 376, 717, 897
HphI	GGTGA	1 cut(s)	454
Hpy166II	GTNNAC	2 cut(s)	609, 915
Hpy188I	TCNGA	6 cut(s)	33, 306, 395, 489, 570, 771
Hpy188III	TCNNGA	6 cut(s)	160, 253, 407, 530, 823, 883
Hpy8I	GTNNAC	2 cut(s)	609, 915
Hpy99I	CGWCG	1 cut(s)	37
HpyAV	CCTTC	7 cut(s)	88, 250, 280, 352, 530, 647, 836
HpyCH4III	ACNGT	4 cut(s)	223, 512, 574, 919
HpyCH4IV	ACGT	1 cut(s)	90
HpyCH4V	TGCA	4 cut(s)	328, 707, 729, 968
HpyF3I	CTNAG	1 cut(s)	768
HpySE526I	ACGT	1 cut(s)	90
Hsp92I	GRCGYC	1 cut(s)	216
Hsp92II	CATG	8 cut(s)	127, 374, 607, 653, 681, 811, 818, 886
HspAI	GCGC	4 cut(s)	216, 299, 333, 580
KasI	GGCGCC	1 cut(s)	215
KflI	GGGWCCC	1 cut(s)	734
KroI	GCCGGC	2 cut(s)	375, 716
KroNI	GCCGGC	2 cut(s)	377, 718
Kzo9I	GATC	2 cut(s)	522, 819
LmnI	GCTCC	4 cut(s)	174, 317, 395, 571
Lsp1109I	GCAGC	5 cut(s)	312, 472, 550, 586, 660
LweI	GCATC	1 cut(s)	129
MaeII	ACGT	1 cut(s)	90
MaeIII	GTNAC	1 cut(s)	424
MalI	GATC	2 cut(s)	524, 821
MboI	GATC	2 cut(s)	522, 819
MboII	GAAGA	3 cut(s)	17, 655, 950
MhlI	GDGCHC	4 cut(s)	41, 250, 314, 517
MluCI	AATT	1 cut(s)	447
Mly113I	GGCGCC	1 cut(s)	216
MlyI	GAGTC	4 cut(s)	249, 484, 675, 861
MmeI	TCCRAC	3 cut(s)	56, 339, 593
MnlI	CCTC	9 cut(s)	121, 229, 430, 526, 750, 770, 832, 879, 885
MroNI	GCCGGC	2 cut(s)	375, 716
MroXI	GAANNNNTTC	1 cut(s)	846
MseI	TTAA	1 cut(s)	339
MspI	CCGG	8 cut(s)	166, 181, 186, 244, 354, 376, 717, 897
MspR9I	CCNGG	6 cut(s)	166, 245, 355, 596, 897, 898
MvaI	CCWGG	1 cut(s)	596
MvnI	CGCG	4 cut(s)	134, 299, 335, 347
NaeI	GCCGGC	2 cut(s)	377, 718
NarI	GGCGCC	1 cut(s)	216
NciI	CCSGG	5 cut(s)	166, 245, 355, 897, 898
NcoI	CCATGG	2 cut(s)	370, 677
NdeII	GATC	2 cut(s)	522, 819
NgoMIV	GCCGGC	2 cut(s)	375, 716
NlaIII	CATG	8 cut(s)	127, 374, 607, 653, 681, 811, 818, 886
NlaIV	GGNNCC	8 cut(s)	170, 190, 217, 313, 501, 721, 735, 736
NmeAIII	GCCGAG	3 cut(s)	93, 800, 856
NmuCI	GTSAC	1 cut(s)	424
NsbI	TGCGCA	1 cut(s)	581
PagI	TCATGA	1 cut(s)	882
PcsI	WCGNNNNNNNCGW	2 cut(s)	414, 958
PdiI	GCCGGC	2 cut(s)	377, 718
PdmI	GAANNNNTTC	1 cut(s)	846
PfeI	GAWTC	3 cut(s)	79, 201, 811
Pfl23II	CGTACG	2 cut(s)	87, 518
PflMI	CCANNNNNTGG	1 cut(s)	912
PleI	GAGTC	4 cut(s)	248, 483, 674, 861
PluTI	GGCGCC	1 cut(s)	219
PpsI	GAGTC	4 cut(s)	248, 483, 674, 861
Ppu21I	YACGTR	1 cut(s)	91
PpuMI	RGGWCCY	2 cut(s)	500, 734
Psp5II	RGGWCCY	2 cut(s)	500, 734
Psp6I	CCWGG	1 cut(s)	594
PspGI	CCWGG	1 cut(s)	594
PspLI	CGTACG	2 cut(s)	87, 518
PspN4I	GGNNCC	8 cut(s)	170, 190, 217, 313, 501, 721, 735, 736
PspPI	GGNCC	6 cut(s)	97, 183, 259, 500, 719, 734
PspPPI	RGGWCCY	2 cut(s)	500, 734
PstI	CTGCAG	1 cut(s)	330
PstNI	CAGNNNCTG	1 cut(s)	563
RsaI	GTAC	3 cut(s)	89, 296, 520
RsaNI	GTAC	3 cut(s)	88, 295, 519
Rsr2I	CGGWCCG	1 cut(s)	183
RsrII	CGGWCCG	1 cut(s)	183
SaqAI	TTAA	1 cut(s)	339
Sau3AI	GATC	2 cut(s)	522, 819
Sau96I	GGNCC	6 cut(s)	97, 183, 259, 500, 719, 734
SchI	GAGTC	4 cut(s)	249, 484, 675, 861
ScrFI	CCNGG	6 cut(s)	166, 245, 355, 596, 897, 898
SduI	GDGCHC	4 cut(s)	41, 250, 314, 517
SfaNI	GCATC	1 cut(s)	129
SfcI	CTRYAG	3 cut(s)	140, 326, 455
SfoI	GGCGCC	1 cut(s)	217
SinI	GGWCC	3 cut(s)	183, 500, 734
SmaI	CCCGGG	1 cut(s)	898
Sse9I	AATT	1 cut(s)	447
SspDI	GGCGCC	1 cut(s)	215
StyD4I	CCNGG	6 cut(s)	164, 243, 353, 594, 895, 896
StyI	CCWWGG	2 cut(s)	370, 677
TaaI	ACNGT	4 cut(s)	223, 512, 574, 919
TaiI	ACGT	1 cut(s)	93
TaqI	TCGA	4 cut(s)	150, 177, 408, 627
TasI	AATT	1 cut(s)	447
TauI	GCSGC	9 cut(s)	52, 55, 58, 61, 64, 76, 120, 134, 216
TfiI	GAWTC	3 cut(s)	79, 201, 811
Tru1I	TTAA	1 cut(s)	339
Tru9I	TTAA	1 cut(s)	339
TscAI	CASTG	2 cut(s)	215, 515
TseFI	GTSAC	1 cut(s)	424
TseI	GCWGC	5 cut(s)	325, 460, 563, 599, 673
Tsp45I	GTSAC	1 cut(s)	424
TspDTI	ATGAA	4 cut(s)	33, 666, 803, 809
TspGWI	ACGGA	1 cut(s)	533
TspMI	CCCGGG	1 cut(s)	896
TspRI	CASTG	2 cut(s)	215, 515
Van91I	CCANNNNNTGG	1 cut(s)	912
VpaK11BI	GGWCC	3 cut(s)	183, 500, 734
XmaI	CCCGGG	1 cut(s)	896
XmnI	GAANNNNTTC	1 cut(s)	846

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.