Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0013658

Physical Location & Seq

Genomic Coordinates Reverse (-)

25755329 .. 25757154

Gene Structure 1826 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1177100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1668 bp

ATGTCGACATCGGGGACGCCGCAGTTCCGGTACACGCAGACGCCGTCGAAGGTGCTCCACCTCCATAACCTCCCGTGGGAGTGCGCCGAGGAGGAGTTGATCGAGCTCTGCAAACCTTTCGGCAAGATCGTCAACACTAAGTGTAATGTCGGCGCCAATCGCAATCAGGCCTTCGTCGAATTCTCGGACCTAAACCAGGCCATCAATATGGTTTCGTATTATGCTTCATCCTCGGAGCCTGCGCAGGTCCGTGGTAAAACCGTGTACATTCAGTATTCAAATAGACACGAAATTGTCAATAACAAAAGTCCGGGAGATGTGCCCGGAAATGTCTTGTTGGTAACCATTGAGGGTGTTGAAGCTGTTGAAGCTGGCGATGTGAGCATTGATGTTATTCACTTGGTGTTCTCTGCTTTCGGCTTTGCATTGATTCAGTTCAGTGATGCTGTCACCGCATCTATGGCAAGGAATGCATTGGATGGAAGAAGTATACCCAGGTACTTGCTTCCAGAGAACGTCGGTTCATGCAACTTACGTATTTCATATTCCGCTCCCCCGATCTGCGAACACCACCGTCACTACACCACAACTGGCTTCCATGCTCCGCTCAGGTGGAGGGTCTTGGGTTCGAAGGTTGTCATCAGCAACGTCAGCGCCAGCAGCGTGATTCTCGACTCCGAGCTCGGCAAGCAACTCCATGCCCACGCTCACAAGCTCGGCTGCTCTGGTGACCGGTACGTGGTTGCTGCTCTCATTGAGTTGTACGGTCGCCTCGACAGCGCGGACTCCGCGAAATGGCCGTTTGACAAATCCCTGGTTAAGGACTATGTTTCATGGACAATGCTGGCCAGGCTGTACATTGCGGAAGGTAAACCCGGCAAGGCGGTTCATGTGTTTGAGAGAATGGTGGAGTCCGGAGCACAGATAGATTCTGTGGCTGTGGCGACGGCGGCTGGTGCCTGTGGCATGTTGAGATCGTTGATTGATGGAACAAAAGTCCACAGGGTTGCCAAGGAACGTGGATTGGGGTATGATGTGTTGGTGAGCAATACCCTCTTGAAAATGTACATGGACTGTGGCTGTGTCGACGAAGCTTGGGCAGTTTTCAACCAAATGCCGGAGAAAGATGTCATTTCTTGCACAGAAATGATTCGTGCCAATGTGAAGAGGGGATGCTTCAATGAGGGTCTTAAGCTGTTTAGGCAGATGGTTGGAGACGGGGTGAAACCTGATCCGCTCTCGGTCTCTAGTGTTCTCCCAGCATGCGCAAGAATGTCTGCAGGTAAACATGGCAAAGAGACTCGTGGATTCTTGCTGCGACATGGTATTCGAATGAATCTCACCGTTCTGAATGCTCTTGTGGACATGTATGTCAAGTCGGGATTTATCAAATCAGCTGCAAAAGTTTTTGCCAGGTTGAAGTACAGAGATCTTGTTTCGTGGACAGTGATGATAACTGGGTACAGCTTACACGGACAAGGAAAGATCGGAGTAGATTTATTCTGCCAAATGGAGAAGGAAACGAGTACACAGATCGATGAAATCACCTATGCTGCTGTCCTTCATGCTTGCGTTGCAGCACGCATGGTCGAGGAAGGGAAGTTTTACTTCAACTGCATCAAGACACCTACAGTTGCACATTGTGCTTTCGGATCCCGTCATGCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

556

Amino Acids

61.01

Weight (kDa)

8.56

Isoelectric Point (pI)

33.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RRM_1	PF00076	22 - 70	3.9e-07	RNA recognition motif
PPR_2	PF13041	375 - 424	1.9e-06	PPR repeat family
PPR_2	PF13041	477 - 525	1.6e-06	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013658)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g22160 FvH4_4g22160
malus_domestica	MD00G1011100.v1.1 MD00G1167900.v1.1 MD02G1251400.v1.1 MD11G1025800.v1.1 MD12G1177100.v1.1
prunus_persica	Prupe.1G211300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0428391
rosa_laevigata	RLG00000007155
rosa_multiflora	Rmu_sc0004698.1_g000005
rosa_roxburghii	Rroxscaffold_5G00370060
rosa_rugosa	Rorug04G0225200
rosa_samantha	Rh4AG282300 Rh4BG287900 Rh4CG303700 Rh4DG285000
rosa_wichuraiana	Rw4G024500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1370
Acc16I	TGCGCA	2 cut(s)	243, 1267
Acc36I	ACCTGC	2 cut(s)	235, 1271
AccB1I	GGYRCC	2 cut(s)	152, 956
AccBSI	CCGCTC	3 cut(s)	551, 607, 1237
AccI	GTMKAC	3 cut(s)	5, 490, 1086
AccII	CGCG	2 cut(s)	782, 791
AccIII	TCCGGA	1 cut(s)	914
AclWI	GGATC	3 cut(s)	1226, 1647, 1660
AcoI	YGGCCR	2 cut(s)	797, 846
AcsI	RAATTY	1 cut(s)	179
AcyI	GRCGYC	3 cut(s)	17, 41, 153
AdeI	CACNNNGTG	3 cut(s)	141, 403, 1643
AfiI	CCNNNNNNNGG	6 cut(s)	76, 196, 739, 795, 820, 1117
AflII	CTTAAG	1 cut(s)	1190
AflIII	ACRYGT	1 cut(s)	1365
AgeI	ACCGGT	1 cut(s)	732
AgsI	TTSAA	8 cut(s)	279, 359, 368, 1060, 1108, 1180, 1420, 1612
AjnI	CCWGG	5 cut(s)	195, 494, 813, 848, 1412
AluBI	AGCT	9 cut(s)	106, 362, 371, 682, 715, 1094, 1195, 1397, 1467
AluI	AGCT	9 cut(s)	106, 362, 371, 682, 715, 1094, 1195, 1397, 1467
Alw21I	GWGCWC	4 cut(s)	57, 108, 684, 922
Alw26I	GTCTC	3 cut(s)	1209, 1249, 1292
AlwI	GGATC	3 cut(s)	1226, 1647, 1660
Aor13HI	TCCGGA	1 cut(s)	914
AoxI	GGCC	4 cut(s)	168, 198, 797, 846
ApeKI	GCWGC	7 cut(s)	660, 720, 746, 1315, 1397, 1553, 1577
ApoI	RAATTY	1 cut(s)	179
ArsI	GACNNNNNNTTYG	2 cut(s)	1311, 1343
AsiGI	ACCGGT	1 cut(s)	732
Asp700I	GAANNNNTTC	1 cut(s)	1149
AspLEI	GCGC	6 cut(s)	86, 155, 244, 656, 782, 1268
AspS9I	GGNCC	2 cut(s)	187, 247
AsuC2I	CCSGG	3 cut(s)	312, 324, 876
AsuHPI	GGTGA	6 cut(s)	442, 740, 1054, 1234, 1333, 1537
AsuII	TTCGAA	2 cut(s)	629, 1330
AvaII	GGWCC	2 cut(s)	187, 247
BaeGI	GKGCMC	1 cut(s)	324
BalI	TGGCCA	1 cut(s)	848
BamHI	GGATCC	1 cut(s)	1652
BanI	GGYRCC	2 cut(s)	152, 956
BanII	GRGCYC	2 cut(s)	108, 684
BauI	CACGAG	1 cut(s)	1302
Bbv12I	GWGCWC	4 cut(s)	57, 108, 684, 922
BbvI	GCAGC	7 cut(s)	672, 707, 733, 1302, 1384, 1540, 1589
BccI	CCATC	4 cut(s)	209, 473, 980, 1201
BceAI	ACGGC	3 cut(s)	28, 784, 963
BcgI	CGANNNNNNTGC	2 cut(s)	100, 134
BciT130I	CCWGG	5 cut(s)	197, 496, 815, 850, 1414
BcnI	CCSGG	3 cut(s)	312, 324, 876
BcoDI	GTCTC	3 cut(s)	1209, 1249, 1292
BfaI	CTAG	1 cut(s)	1248
BfmI	CTRYAG	2 cut(s)	1278, 1629
BfoI	RGCGCY	2 cut(s)	156, 657
BfrI	CTTAAG	1 cut(s)	1190
BfuAI	ACCTGC	2 cut(s)	235, 1271
BglII	AGATCT	1 cut(s)	1429
BisI	GCNGC	9 cut(s)	20, 661, 721, 747, 951, 1316, 1398, 1554, 1578
BlsI	GCNGC	9 cut(s)	21, 662, 722, 748, 952, 1317, 1399, 1555, 1579
Bme1390I	CCNGG	8 cut(s)	197, 312, 324, 496, 815, 850, 876, 1414
Bme18I	GGWCC	2 cut(s)	187, 247
BmgT120I	GGNCC	2 cut(s)	187, 247
BmiI	GGNNCC	4 cut(s)	154, 237, 958, 1654
BmrFI	CCNGG	8 cut(s)	197, 312, 324, 496, 815, 850, 876, 1414
BmrI	ACTGGG	1 cut(s)	1467
BmsI	GCATC	4 cut(s)	433, 464, 1163, 1626
BmuI	ACTGGG	1 cut(s)	1467
Bpu10I	CCTNAGC	1 cut(s)	608
Bpu14I	TTCGAA	2 cut(s)	629, 1330
BpuMI	CCSGG	3 cut(s)	312, 324, 876
Bsa29I	ATCGAT	1 cut(s)	1536
BsaAI	YACGTR	2 cut(s)	536, 739
BsaHI	GRCGYC	3 cut(s)	17, 41, 153
BsaI	GGTCTC	1 cut(s)	1249
BsaJI	CCNNGG	7 cut(s)	74, 87, 231, 250, 494, 813, 1011
BsaWI	WCCGGW	3 cut(s)	27, 732, 914
BsaXI	ACNNNNNCTCC	2 cut(s)	1206, 1236
Bsc4I	CCNNNNNNNGG	6 cut(s)	76, 196, 739, 795, 820, 1117
Bse118I	RCCGGY	1 cut(s)	732
Bse1I	ACTGG	2 cut(s)	595, 1462
Bse3DI	GCAATG	1 cut(s)	858
BseAI	TCCGGA	1 cut(s)	914
BseBI	CCWGG	5 cut(s)	197, 496, 815, 850, 1414
BseCI	ATCGAT	1 cut(s)	1536
BseDI	CCNNGG	7 cut(s)	74, 87, 231, 250, 494, 813, 1011
BseGI	GGATG	3 cut(s)	227, 484, 1178
BseLI	CCNNNNNNNGG	6 cut(s)	76, 196, 739, 795, 820, 1117
BseMI	GCAATG	1 cut(s)	858
BseMII	CTCAG	1 cut(s)	622
BseNI	ACTGG	2 cut(s)	595, 1462
BseRI	GAGGAG	2 cut(s)	104, 107
BseSI	GKGCMC	1 cut(s)	324
BseXI	GCAGC	7 cut(s)	672, 707, 733, 1302, 1384, 1540, 1589
BseYI	CCCAGC	1 cut(s)	1258
Bsh1236I	CGCG	2 cut(s)	782, 791
Bsh1285I	CGRYCG	1 cut(s)	769
BshFI	GGCC	4 cut(s)	170, 200, 799, 848
BshNI	GGYRCC	2 cut(s)	152, 956
BshTI	ACCGGT	1 cut(s)	732
BshVI	ATCGAT	1 cut(s)	1536
BsiEI	CGRYCG	1 cut(s)	769
BsiHKAI	GWGCWC	4 cut(s)	57, 108, 684, 922
BsiSI	CCGG	7 cut(s)	28, 311, 324, 733, 876, 915, 1118
BslFI	GGGAC	1 cut(s)	28
BslI	CCNNNNNNNGG	6 cut(s)	76, 196, 739, 795, 820, 1117
BsmAI	GTCTC	3 cut(s)	1209, 1249, 1292
BsmBI	CGTCTC	1 cut(s)	1209
BsmFI	GGGAC	1 cut(s)	28
BsmI	GAATGC	2 cut(s)	475, 1357
BsnI	GGCC	4 cut(s)	170, 200, 799, 848
Bso31I	GGTCTC	1 cut(s)	1249
Bsp119I	TTCGAA	2 cut(s)	629, 1330
Bsp1286I	GDGCHC	5 cut(s)	57, 108, 324, 684, 922
Bsp13I	TCCGGA	1 cut(s)	914
Bsp1407I	TGTACA	3 cut(s)	264, 855, 1065
Bsp143I	GATC	9 cut(s)	99, 126, 558, 974, 1231, 1429, 1485, 1533, 1652
BspANI	GGCC	4 cut(s)	170, 200, 799, 848
BspCNI	CTCAG	1 cut(s)	621
BspDI	ATCGAT	1 cut(s)	1536
BspEI	TCCGGA	1 cut(s)	914
BspFNI	CGCG	2 cut(s)	782, 791
BspLI	GGNNCC	4 cut(s)	154, 237, 958, 1654
BspMAI	CTGCAG	1 cut(s)	1282
BspMI	ACCTGC	2 cut(s)	235, 1271
BspPI	GGATC	3 cut(s)	1226, 1647, 1660
BspT104I	TTCGAA	2 cut(s)	629, 1330
BspT107I	GGYRCC	2 cut(s)	152, 956
BspTI	CTTAAG	1 cut(s)	1190
BspTNI	GGTCTC	1 cut(s)	1249
BsrBI	CCGCTC	3 cut(s)	551, 607, 1237
BsrDI	GCAATG	1 cut(s)	858
BsrFI	RCCGGY	1 cut(s)	732
BsrGI	TGTACA	3 cut(s)	264, 855, 1065
BsrI	ACTGG	2 cut(s)	595, 1462
BssAI	RCCGGY	1 cut(s)	732
BssECI	CCNNGG	7 cut(s)	74, 87, 231, 250, 494, 813, 1011
BssMI	GATC	9 cut(s)	99, 126, 558, 974, 1231, 1429, 1485, 1533, 1652
BssNAI	GTATAC	1 cut(s)	491
BssNI	GRCGYC	3 cut(s)	17, 41, 153
BssSI	CACGAG	1 cut(s)	1302
BssT1I	CCWWGG	1 cut(s)	1011
Bst1107I	GTATAC	1 cut(s)	491
Bst2BI	CACGAG	1 cut(s)	1302
Bst2UI	CCWGG	5 cut(s)	197, 496, 815, 850, 1414
Bst4CI	ACNGT	7 cut(s)	262, 575, 767, 1076, 1345, 1447, 1633
Bst6I	CTCTTC	1 cut(s)	1160
BstACI	GRCGYC	3 cut(s)	17, 41, 153
BstAFI	CTTAAG	1 cut(s)	1190
BstAPI	GCANNNNNTGC	2 cut(s)	470, 1643
BstAUI	TGTACA	3 cut(s)	264, 855, 1065
BstBAI	YACGTR	2 cut(s)	536, 739
BstBI	TTCGAA	2 cut(s)	629, 1330
BstC8I	GCNNGC	8 cut(s)	240, 373, 658, 689, 846, 1264, 1570, 1582
BstDEI	CTNAG	2 cut(s)	138, 608
BstDSI	CCRYGG	2 cut(s)	74, 250
BstEII	GGTNACC	2 cut(s)	340, 728
BstENI	CCTNNNNNAGG	2 cut(s)	194, 818
BstF5I	GGATG	3 cut(s)	227, 484, 1178
BstFNI	CGCG	2 cut(s)	782, 791
BstH2I	RGCGCY	2 cut(s)	156, 657
BstHHI	GCGC	6 cut(s)	86, 155, 244, 656, 782, 1268
BstKTI	GATC	9 cut(s)	102, 129, 561, 977, 1234, 1432, 1488, 1536, 1655
BstMAI	GTCTC	3 cut(s)	1209, 1249, 1292
BstMBI	GATC	9 cut(s)	99, 126, 558, 974, 1231, 1429, 1485, 1533, 1652
BstMCI	CGRYCG	1 cut(s)	769
BstNI	CCWGG	5 cut(s)	197, 496, 815, 850, 1414
BstNSI	RCATGY	3 cut(s)	970, 1266, 1369
BstPI	GGTNACC	2 cut(s)	340, 728
BstSCI	CCNGG	8 cut(s)	195, 310, 322, 494, 813, 848, 874, 1412
BstSFI	CTRYAG	2 cut(s)	1278, 1629
BstSLI	GKGCMC	1 cut(s)	324
BstSNI	TACGTA	1 cut(s)	536
BstUI	CGCG	2 cut(s)	782, 791
BstV1I	GCAGC	7 cut(s)	672, 707, 733, 1302, 1384, 1540, 1589
BstX2I	RGATCY	2 cut(s)	1429, 1652
BstXI	CCANNNNNNTGG	1 cut(s)	208
BstYI	RGATCY	2 cut(s)	1429, 1652
BstZ17I	GTATAC	1 cut(s)	491
Bsu15I	ATCGAT	1 cut(s)	1536
BsuRI	GGCC	4 cut(s)	170, 200, 799, 848
BsuTUI	ATCGAT	1 cut(s)	1536
BtgI	CCRYGG	2 cut(s)	74, 250
BtgZI	GCGATG	1 cut(s)	390
BtsCI	GGATG	3 cut(s)	227, 484, 1178
BtsIMutI	CAGTG	2 cut(s)	445, 1452
BveI	ACCTGC	2 cut(s)	235, 1271
Cac8I	GCNNGC	8 cut(s)	240, 373, 658, 689, 846, 1264, 1570, 1582
CfoI	GCGC	6 cut(s)	86, 155, 244, 656, 782, 1268
Cfr10I	RCCGGY	1 cut(s)	732
Cfr13I	GGNCC	2 cut(s)	187, 247
ClaI	ATCGAT	1 cut(s)	1536
CseI	GACGC	2 cut(s)	25, 49
CspAI	ACCGGT	1 cut(s)	732
CspCI	CAANNNNNGTGG	2 cut(s)	1000, 1035
DdeI	CTNAG	2 cut(s)	138, 608
DinI	GGCGCC	1 cut(s)	154
DpnI	GATC	9 cut(s)	101, 128, 560, 976, 1233, 1431, 1487, 1535, 1654
DpnII	GATC	9 cut(s)	99, 126, 558, 974, 1231, 1429, 1485, 1533, 1652
DraIII	CACNNNGTG	3 cut(s)	141, 403, 1643
DrdI	GACNNNNNNGTC	1 cut(s)	1370
DseDI	GACNNNNNNGTC	1 cut(s)	1370
EaeI	YGGCCR	2 cut(s)	797, 846
Eam1104I	CTCTTC	1 cut(s)	1160
EarI	CTCTTC	1 cut(s)	1160
Ecl136II	GAGCTC	2 cut(s)	106, 682
Eco105I	TACGTA	1 cut(s)	536
Eco130I	CCWWGG	1 cut(s)	1011
Eco147I	AGGCCT	1 cut(s)	170
Eco24I	GRGCYC	2 cut(s)	108, 684
Eco31I	GGTCTC	1 cut(s)	1249
Eco47I	GGWCC	2 cut(s)	187, 247
Eco53kI	GAGCTC	2 cut(s)	106, 682
Eco91I	GGTNACC	2 cut(s)	340, 728
EcoICRI	GAGCTC	2 cut(s)	106, 682
EcoNI	CCTNNNNNAGG	2 cut(s)	194, 818
EcoO65I	GGTNACC	2 cut(s)	340, 728
EcoRI	GAATTC	1 cut(s)	179
EcoRII	CCWGG	5 cut(s)	195, 494, 813, 848, 1412
EcoT14I	CCWWGG	1 cut(s)	1011
EcoT22I	ATGCAT	1 cut(s)	475
EcoT38I	GRGCYC	2 cut(s)	108, 684
EgeI	GGCGCC	1 cut(s)	154
EheI	GGCGCC	1 cut(s)	154
ErhI	CCWWGG	1 cut(s)	1011
Esp3I	CGTCTC	1 cut(s)	1209
FalI	AAGNNNNNCTT	2 cut(s)	1592, 1624
FaqI	GGGAC	1 cut(s)	28
FblI	GTMKAC	3 cut(s)	5, 490, 1086
Fnu4HI	GCNGC	9 cut(s)	20, 661, 721, 747, 951, 1316, 1398, 1554, 1578
FokI	GGATG	3 cut(s)	214, 491, 1185
FriOI	GRGCYC	2 cut(s)	108, 684
Fsp4HI	GCNGC	9 cut(s)	20, 661, 721, 747, 951, 1316, 1398, 1554, 1578
FspBI	CTAG	1 cut(s)	1248
FspI	TGCGCA	2 cut(s)	243, 1267
GlaI	GCGC	6 cut(s)	85, 154, 243, 655, 781, 1267
GluI	GCNGC	9 cut(s)	20, 661, 721, 747, 951, 1316, 1398, 1554, 1578
GsaI	CCCAGC	1 cut(s)	1262
HaeII	RGCGCY	2 cut(s)	156, 657
HaeIII	GGCC	4 cut(s)	170, 200, 799, 848
HapII	CCGG	7 cut(s)	28, 311, 324, 733, 876, 915, 1118
HgaI	GACGC	2 cut(s)	25, 49
HhaI	GCGC	6 cut(s)	86, 155, 244, 656, 782, 1268
Hin1I	GRCGYC	3 cut(s)	17, 41, 153
Hin6I	GCGC	6 cut(s)	84, 153, 242, 654, 780, 1266
HinP1I	GCGC	6 cut(s)	84, 153, 242, 654, 780, 1266
HincII	GTYRAC	3 cut(s)	6, 133, 1087
HindII	GTYRAC	3 cut(s)	6, 133, 1087
HindIII	AAGCTT	1 cut(s)	1092
HpaII	CCGG	7 cut(s)	28, 311, 324, 733, 876, 915, 1118
HphI	GGTGA	6 cut(s)	442, 740, 1054, 1234, 1333, 1537
Hpy188I	TCNGA	6 cut(s)	187, 235, 679, 1350, 1490, 1652
Hpy188III	TCNNGA	6 cut(s)	509, 671, 915, 1057, 1380, 1621
Hpy99I	CGWCG	5 cut(s)	49, 179, 521, 949, 1091
HpyAV	CCTTC	7 cut(s)	43, 181, 625, 860, 1510, 1571, 1589
HpyCH4III	ACNGT	7 cut(s)	262, 575, 767, 1076, 1345, 1447, 1633
HpyCH4IV	ACGT	5 cut(s)	516, 535, 648, 738, 1018
HpyF3I	CTNAG	2 cut(s)	138, 608
HpySE526I	ACGT	5 cut(s)	516, 535, 648, 738, 1018
Hsp92I	GRCGYC	3 cut(s)	17, 41, 153
HspAI	GCGC	6 cut(s)	84, 153, 242, 654, 780, 1266
KasI	GGCGCC	1 cut(s)	152
Kpn2I	TCCGGA	1 cut(s)	914
Kzo9I	GATC	9 cut(s)	99, 126, 558, 974, 1231, 1429, 1485, 1533, 1652
LmnI	GCTCC	5 cut(s)	60, 235, 556, 607, 917
Lsp1109I	GCAGC	7 cut(s)	672, 707, 733, 1302, 1384, 1540, 1589
LweI	GCATC	4 cut(s)	433, 464, 1163, 1626
MaeI	CTAG	1 cut(s)	1248
MaeII	ACGT	5 cut(s)	516, 535, 648, 738, 1018
MaeIII	GTNAC	4 cut(s)	340, 448, 575, 728
MalI	GATC	9 cut(s)	101, 128, 560, 976, 1233, 1431, 1487, 1535, 1654
MbiI	CCGCTC	3 cut(s)	551, 607, 1237
MboI	GATC	9 cut(s)	99, 126, 558, 974, 1231, 1429, 1485, 1533, 1652
MboII	GAAGA	2 cut(s)	495, 1177
MflI	RGATCY	2 cut(s)	1429, 1652
MhlI	GDGCHC	5 cut(s)	57, 108, 324, 684, 922
MlsI	TGGCCA	1 cut(s)	848
MluCI	AATT	2 cut(s)	179, 291
MluNI	TGGCCA	1 cut(s)	848
Mly113I	GGCGCC	1 cut(s)	153
MlyI	GAGTC	4 cut(s)	668, 779, 920, 1294
MmeI	TCCRAC	1 cut(s)	1192
Mox20I	TGGCCA	1 cut(s)	848
Mph1103I	ATGCAT	1 cut(s)	475
MroI	TCCGGA	1 cut(s)	914
MroXI	GAANNNNTTC	1 cut(s)	1149
MscI	TGGCCA	1 cut(s)	848
MseI	TTAA	2 cut(s)	819, 1191
MslI	CAYNNNNRTG	2 cut(s)	206, 1145
Msp20I	TGGCCA	1 cut(s)	848
MspA1I	CMGCKG	1 cut(s)	1397
MspCI	CTTAAG	1 cut(s)	1190
MspI	CCGG	7 cut(s)	28, 311, 324, 733, 876, 915, 1118
MspR9I	CCNGG	8 cut(s)	197, 312, 324, 496, 815, 850, 876, 1414
Mva1269I	GAATGC	2 cut(s)	475, 1357
MvaI	CCWGG	5 cut(s)	197, 496, 815, 850, 1414
MvnI	CGCG	2 cut(s)	782, 791
NarI	GGCGCC	1 cut(s)	153
NciI	CCSGG	3 cut(s)	312, 324, 876
NdeII	GATC	9 cut(s)	99, 126, 558, 974, 1231, 1429, 1485, 1533, 1652
NlaIV	GGNNCC	4 cut(s)	154, 237, 958, 1654
NmeAIII	GCCGAG	3 cut(s)	112, 663, 696
NmuCI	GTSAC	3 cut(s)	448, 575, 728
NsbI	TGCGCA	2 cut(s)	243, 1267
NsiI	ATGCAT	1 cut(s)	475
NspI	RCATGY	3 cut(s)	970, 1266, 1369
NspV	TTCGAA	2 cut(s)	629, 1330
PaeI	GCATGC	1 cut(s)	1266
PceI	AGGCCT	1 cut(s)	170
PciI	ACATGT	1 cut(s)	1365
PcsI	WCGNNNNNNNCGW	3 cut(s)	41, 126, 771
PctI	GAATGC	2 cut(s)	475, 1357
PdmI	GAANNNNTTC	1 cut(s)	1149
PfeI	GAWTC	6 cut(s)	430, 667, 929, 1150, 1308, 1336
PflFI	GACNNNGTC	1 cut(s)	43
PfoI	TCCNGGA	1 cut(s)	310
PinAI	ACCGGT	1 cut(s)	732
PkrI	GCNGC	9 cut(s)	21, 662, 722, 748, 952, 1317, 1399, 1555, 1579
PleI	GAGTC	4 cut(s)	668, 779, 919, 1294
PluTI	GGCGCC	1 cut(s)	156
PpsI	GAGTC	4 cut(s)	668, 779, 919, 1294
Ppu21I	YACGTR	2 cut(s)	536, 739
PscI	ACATGT	1 cut(s)	1365
Psp124BI	GAGCTC	2 cut(s)	108, 684
Psp6I	CCWGG	5 cut(s)	195, 494, 813, 848, 1412
PspEI	GGTNACC	2 cut(s)	340, 728
PspFI	CCCAGC	1 cut(s)	1258
PspGI	CCWGG	5 cut(s)	195, 494, 813, 848, 1412
PspN4I	GGNNCC	4 cut(s)	154, 237, 958, 1654
PspPI	GGNCC	2 cut(s)	187, 247
PstI	CTGCAG	1 cut(s)	1282
PsuI	RGATCY	2 cut(s)	1429, 1652
PsyI	GACNNNGTC	1 cut(s)	43
PvuII	CAGCTG	1 cut(s)	1397
RseI	CAYNNNNRTG	2 cut(s)	206, 1145
SacI	GAGCTC	2 cut(s)	108, 684
SalI	GTCGAC	2 cut(s)	4, 1085
SaqAI	TTAA	2 cut(s)	819, 1191
SatI	GCNGC	9 cut(s)	20, 661, 721, 747, 951, 1316, 1398, 1554, 1578
Sau3AI	GATC	9 cut(s)	99, 126, 558, 974, 1231, 1429, 1485, 1533, 1652
Sau96I	GGNCC	2 cut(s)	187, 247
SchI	GAGTC	4 cut(s)	668, 779, 920, 1294
ScrFI	CCNGG	8 cut(s)	197, 312, 324, 496, 815, 850, 876, 1414
SduI	GDGCHC	5 cut(s)	57, 108, 324, 684, 922
SfaNI	GCATC	4 cut(s)	433, 464, 1163, 1626
SfcI	CTRYAG	2 cut(s)	1278, 1629
SfoI	GGCGCC	1 cut(s)	154
SfuI	TTCGAA	2 cut(s)	629, 1330
SinI	GGWCC	2 cut(s)	187, 247
SmiMI	CAYNNNNRTG	2 cut(s)	206, 1145
SmlI	CTYRAG	1 cut(s)	1190
SmoI	CTYRAG	1 cut(s)	1190
SnaBI	TACGTA	1 cut(s)	536
SphI	GCATGC	1 cut(s)	1266
Sse9I	AATT	2 cut(s)	179, 291
SseBI	AGGCCT	1 cut(s)	170
SspDI	GGCGCC	1 cut(s)	152
SspMI	CTAG	1 cut(s)	1248
SstI	GAGCTC	2 cut(s)	108, 684
StuI	AGGCCT	1 cut(s)	170
StyD4I	CCNGG	8 cut(s)	195, 310, 322, 494, 813, 848, 874, 1412
StyI	CCWWGG	1 cut(s)	1011
TaaI	ACNGT	7 cut(s)	262, 575, 767, 1076, 1345, 1447, 1633
TaiI	ACGT	5 cut(s)	519, 538, 651, 741, 1021
TaqII	GACCGA	1 cut(s)	1231
TasI	AATT	2 cut(s)	179, 291
TatI	WGTACW	5 cut(s)	264, 855, 1065, 1422, 1526
TauI	GCSGC	2 cut(s)	22, 953
TfiI	GAWTC	6 cut(s)	430, 667, 929, 1150, 1308, 1336
Tru1I	TTAA	2 cut(s)	819, 1191
Tru9I	TTAA	2 cut(s)	819, 1191
TscAI	CASTG	2 cut(s)	445, 1452
TseFI	GTSAC	3 cut(s)	448, 575, 728
TseI	GCWGC	7 cut(s)	660, 720, 746, 1315, 1397, 1553, 1577
Tsp45I	GTSAC	3 cut(s)	448, 575, 728
TspDTI	ATGAA	8 cut(s)	216, 513, 531, 822, 878, 1349, 1553, 1554
TspGWI	ACGGA	2 cut(s)	239, 1488
TspRI	CASTG	2 cut(s)	445, 1452
Tth111I	GACNNNGTC	1 cut(s)	43
Vha464I	CTTAAG	1 cut(s)	1190
VpaK11BI	GGWCC	2 cut(s)	187, 247
XagI	CCTNNNNNAGG	2 cut(s)	194, 818
XapI	RAATTY	1 cut(s)	179
XceI	RCATGY	3 cut(s)	970, 1266, 1369
XmiI	GTMKAC	3 cut(s)	5, 490, 1086
XmnI	GAANNNNTTC	1 cut(s)	1149
XspI	CTAG	1 cut(s)	1248
Zsp2I	ATGCAT	1 cut(s)	475

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.