Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0003312

Physical Location & Seq

Genomic Coordinates Reverse (-)

29445190 .. 29449610

Gene Structure 4421 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1216900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2805 bp

ATGGAGATGGAATCAGATCAGCCAGATGATGTTGGTAATTGCATCGAAGAGAAATCTGTTCAGCATTTACTTGATCCAGGTAGTCCTGATATAAGCAATATGTTCGGAGAGCAGCAAGTGGCTCCCCGAGTTGGTGATGCATACCAAGTGGAAATCCCTTCCCTGACTATGGACGCTGAGCAGTATAAGCTTTTGACTAATCCTGCTCATTCAAAAGTTGTTGATGGTTCCCATTACTTTCTAGTTGGTTTGCCCGTACCAATTGTATATTCTGACGAAGTGAATAACATTGAAGATCGCAGGCTGGAATCTCCAATTAACCCTGATAATGTGGTTAATGGAAAGAGTTCTAAGGAAGCTAGAAAGAGAAAGAAGGATCCTACTAGACTGAGGAAGAAAAGTTCAAAACTCAAAGTTGCAGCCTTGTTATTTGGGTTGGACAAGGTAGAAGAATCAAAAGCAGAAAATATTGAGCCGCCGGTGGTGGAAGAGAATGCAAATCAGTCGCTGAGAAGCAAGTGCTGTTATCCAGTTCCCGGTTCATCAAGAAGCACTTGGAGTGATGCTGAAGTGGATAGCTTTCTTCTGGGTTTATACATTTTCGGGAAGAGCTTTTACCAGGTGAAGAGATTCATGGAGAACAAAGCCATGGGAGAAATACTGTCATTTTATTATGGGAAGTTTTATAGGTCTGATGCACACCGCAGATGGTCAGAGTGCCGGAAAAGTAGAAGAAAGAAATGTATACTTGGGGAGAAAATTTTCACAGGGTGGAGGCAACGGGAGCTGTTATCTCGTATAATTCCTCATGTCTCAGAAGAATCTCAAAAATGTTTATCAGAGGGTTACAAATTATTTGCAGAGGGGACAACGTCTCTAGAAGAATATGTATCCTTCTTAAAGTCCATTGTTGGCATTCGTGTCCTTGTGGTAGCTATAGGAATTGGGAAAGGGAAAGAAGATCTCACAGGCTTTGCCCTGGAAGCTGGAAAGAACAATCAGGAGCTTCCAGCTTGTCCAAAATTACCAGCTTGCAAAGCATTTAGTTCTCTTACATTCAGTGAAATAATTAAATATTTGACCGGAGGAGTTCGGTTGAGCAAAGGGCGATCTAACGATATCTTTTGGGAGGCTGTTTGGCCGCGTTTGCTAGCAAATGGATGGCATTCTGAGCAACCTACATATTCTTTGGTTTTTCTTATGCCTGGCATAAAGAAGTTCAAAAGAAGAAAATTGACAAGGGGAGATCAGTATTTTGATTCTGTTAGCGATGTTCTGAACAAAGTGGCATCTGAACCAGAACTTATTCAACTTCAAGGTGAAGAAGAACTTGTTCCAGAGGCGACATCAGACCAGGATGATCTATCTAATCATCAACGCCATTGCTACCTCAAACCAAGGGTCGTTACTAGCAAACCAAACCGCATGCAGTTCACTGTTGTTGATACCAGTTTGGTCCATGGAGCAAAATCACGTGGCATAGTGGAACTGAAATACTCACCAGTTGAACTCCAAAGTAATTCAGAACAAACCAATTGTTCAAGAGAAAATGAAGGGGACTCTTGTGAGAATAAATTAAATGGACATGAAAGTGATACTGCTGAAATGCAATTGAACAGTAAAACCAATATGGCCAAGCACCTGAAGCGTAAGAGGTTCACAATTGTCGATACCAGTCTAGTTCATAGAGGAAAATCATCAACAGTTACAGAACTGAGATGCTCGCCAACTGTGTTTGAAAGTGTTTCTAAGTCAACTGGTCTTTTACAGGAAACTGAAGAAAATTCGTCTGCGGATTTACTGGCCAAGCATAAGCCTGATGCTGCTGATATTTCATTGGATGGTGAAGTGAATAATTTCAGCGACAACTGCCATAGGGATATATCTGATATTGGTGGTACAAACCAAATGGAAGACAAAAATAGCTCAGATTCTACAGAAAAAATAGAAGGCCACCCGAATAAAAAAAAGGGGATGTCTGATGACAAGAAGCCGAAGAGGACTACATTACTTAAGTTTAGTCGGAAACAGAAATATAGACATTCAAATTCTGTAGGTTCTCTCCCCAAACGCCGAAGATTAGCTGCTTGTGCTAAGGCAGAAACAGGTTGCCTCGTCAACAGTAGCTCTCAAGGTTTGCAGTCAGAACAAGTGGGATCCCATGGCCCATTGAATTCTCTAGATGCAGGCGAGCTTGTTGTTTCTCTAGCGGGTCCTCTTGAAAGGGAGCCATCAATTGCTTCTCTTGCTGAAGGTAGTCCAGTAAGGGAGAGTAGTTCTGAAACTCATGGTGGGAATTGTTCAGGTGAGCGCATGTCGCATGAGAAGAATGAGAATCATCAAGACCAGGAATCATCCATCCTAAACCTACATCAGGATTTGATGGACAGTAGAAACAGTGAAAACATTGTTGTGTTCAACTTGGAAACAAATGTAGACAGTCTGTGCCTCTCATCAACTGAAGCACATGTTGTAGACACTTTGAGTACCTCTAACATGATCTCTAGGAGGCAAAGCACAAGAAACCGACCGCTAACAGCTAGAGCATTGGAAGCTCTTGCAGACGGGTTATTAGGAATTAAAAAGAGAAAAAACCACTCAGAAGATGTCCCTGGGGAAAATCAAGTATCAAGACCCTCCCGCAAGGCCCGGAAGAGAGTCAGGGTAACTTCAAGTCATGCTGATACTGTTAGTGGAATTGTGGCTTCAGAAGAAAATGAGGTGAACGAAGGTTTGAATGTTAGCAAAGATACGGATAGCAAACCTCTTGATCAAACTGGAGAAAAGTGGCCAACTAGCCACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

935

Amino Acids

103.98

Weight (kDa)

6.96

Isoelectric Point (pI)

50.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF7952	PF25826	186 - 316	5.4e-60	Domain of unknown function (DUF7952)
DUF7650	PF24662	354 - 433	4.9e-37	Domain of unknown function (DUF7650)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003312)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09040 AT1G09050 AT1G09050 AT1G55050 AT1G55050
fragaria_vesca	FvH4_6g06260 FvH4_6g06260 FvH4_6g06260 FvH4_6g06260 FvH4_6g06260 FvH4_6g06260 FvH4_6g06260 FvH4_6g06260 FvH4_6g06260 FvH4_6g06260
malus_domestica	MD04G1203200.v1.1 MD12G1216900.v1.1
prunus_persica	Prupe.6G323200_v2.0.a1 Prupe.6G323200_v2.0.a1 Prupe.6G323200_v2.0.a1 Prupe.6G323200_v2.0.a1 Prupe.6G323200_v2.0.a1
pyrus_communis	pycom04g17940 pycom12g20240
rosa_chinensis	RchiOBHm_Chr3g0455281
rosa_laevigata	RLG00000025372
rosa_multiflora	Rmu_sc0002169.1_g000039 Rmu_sc0002169.1_g000040 Rmu_sc0029206.1_g000001
rosa_roxburghii	Rroxscaffold_6G00424400
rosa_rugosa	Rorug03G0005900
rosa_samantha	Rh3AG065700 Rh3BG067900 Rh3CG066300 Rh3DG067900
rosa_wichuraiana	Rw3G005180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	3 cut(s)	745, 2436, 2475
AccII	CGCG	1 cut(s)	1144
AciI	CCGC	8 cut(s)	476, 703, 1142, 1423, 1793, 2210, 2531, 2641
AclWI	GGATC	5 cut(s)	68, 371, 384, 2151, 2164
AcoI	YGGCCR	4 cut(s)	1139, 1632, 1803, 2789
AcsI	RAATTY	4 cut(s)	759, 1783, 2047, 2173
AcuI	CTGAAG	6 cut(s)	588, 1664, 1797, 2271, 2481, 2691
AcvI	CACGTG	1 cut(s)	1475
AdeI	CACNNNGTG	1 cut(s)	771
AfaI	GTAC	3 cut(s)	258, 1900, 2488
AfiI	CCNNNNNNNGG	4 cut(s)	131, 169, 536, 2374
AflII	CTTAAG	1 cut(s)	2012
AflIII	ACRYGT	1 cut(s)	2467
AjnI	CCWGG	7 cut(s)	76, 618, 978, 1204, 1353, 2346, 2611
AjuI	GAANNNNNNNTTGG	2 cut(s)	253, 285
AloI	GAACNNNNNNTCC	2 cut(s)	385, 417
Alw26I	GTCTC	2 cut(s)	817, 879
AlwI	GGATC	5 cut(s)	68, 371, 384, 2151, 2164
AlwNI	CAGNNNCTG	1 cut(s)	508
Ama87I	CYCGRG	1 cut(s)	126
AoxI	GGCC	7 cut(s)	1139, 1632, 1803, 1951, 2164, 2646, 2789
ApeKI	GCWGC	4 cut(s)	112, 419, 1823, 2084
ApoI	RAATTY	4 cut(s)	759, 1783, 2047, 2173
ArsI	GACNNNNNNTTYG	2 cut(s)	1386, 1418
Asp700I	GAANNNNTTC	6 cut(s)	346, 629, 761, 1305, 1332, 1856
AspLEI	GCGC	1 cut(s)	2313
AspS9I	GGNCC	4 cut(s)	1456, 2165, 2213, 2647
AsuC2I	CCSGG	2 cut(s)	537, 2650
AsuHPI	GGTGA	7 cut(s)	146, 634, 1331, 1491, 1856, 2318, 2734
AsuNHI	GCTAGC	1 cut(s)	1150
AvaI	CYCGRG	1 cut(s)	126
AvaII	GGWCC	2 cut(s)	1456, 2213
BalI	TGGCCA	3 cut(s)	1634, 1805, 2791
BamHI	GGATCC	2 cut(s)	376, 2156
BarI	GAAGNNNNNNTAC	2 cut(s)	599, 631
BbrPI	CACGTG	1 cut(s)	1475
BbsI	GAAGAC	1 cut(s)	1920
BbvI	GCAGC	4 cut(s)	124, 431, 1810, 2071
BccI	CCATC	7 cut(s)	218, 702, 1155, 1835, 2239, 2366, 2377
BcgI	CGANNNNNNTGC	4 cut(s)	85, 119, 486, 520
BciT130I	CCWGG	7 cut(s)	78, 620, 980, 1206, 1355, 2348, 2613
BciVI	GTATCC	1 cut(s)	901
BclI	TGATCA	1 cut(s)	2770
BcnI	CCSGG	2 cut(s)	537, 2650
BcoDI	GTCTC	2 cut(s)	817, 879
BfmI	CTRYAG	3 cut(s)	936, 1935, 2052
BfrI	CTTAAG	1 cut(s)	2012
BfuI	GTATCC	1 cut(s)	901
BglII	AGATCT	1 cut(s)	961
BisI	GCNGC	6 cut(s)	113, 420, 476, 1142, 1824, 2085
BlpI	GCTNAGC	1 cut(s)	177
BlsI	GCNGC	6 cut(s)	114, 421, 477, 1143, 1825, 2086
Bme1390I	CCNGG	9 cut(s)	78, 537, 620, 980, 1206, 1355, 2348, 2613, 2650
Bme18I	GGWCC	2 cut(s)	1456, 2213
BmeT110I	CYCGRG	1 cut(s)	126
BmgT120I	GGNCC	4 cut(s)	1456, 2165, 2213, 2647
BmiI	GGNNCC	6 cut(s)	123, 229, 378, 2158, 2214, 2229
BmrFI	CCNGG	9 cut(s)	78, 537, 620, 980, 1206, 1355, 2348, 2613, 2650
BmsI	GCATC	8 cut(s)	51, 127, 553, 685, 1298, 1709, 1810, 2173
BmtI	GCTAGC	1 cut(s)	1154
BpiI	GAAGAC	1 cut(s)	1920
BpmI	CTGGAG	1 cut(s)	2799
Bpu10I	CCTNAGC	1 cut(s)	2094
Bpu1102I	GCTNAGC	1 cut(s)	177
BpuEI	CTTGAG	1 cut(s)	2115
BpuMI	CCSGG	2 cut(s)	537, 2650
BsaAI	YACGTR	1 cut(s)	1475
BsaJI	CCNNGG	7 cut(s)	648, 978, 1397, 1459, 2161, 2611, 2612
BsaWI	WCCGGW	1 cut(s)	1082
Bsc4I	CCNNNNNNNGG	4 cut(s)	131, 169, 536, 2374
Bse118I	RCCGGY	1 cut(s)	478
Bse1I	ACTGG	8 cut(s)	530, 1449, 1502, 1674, 1762, 1806, 2261, 2782
Bse3DI	GCAATG	1 cut(s)	1381
BseBI	CCWGG	7 cut(s)	78, 620, 980, 1206, 1355, 2348, 2613
BseDI	CCNNGG	7 cut(s)	648, 978, 1397, 1459, 2161, 2611, 2612
BseGI	GGATG	6 cut(s)	1166, 1363, 1846, 1980, 2354, 2358
BseLI	CCNNNNNNNGG	4 cut(s)	131, 169, 536, 2374
BseMI	GCAATG	1 cut(s)	1381
BseMII	CTCAG	8 cut(s)	168, 380, 500, 828, 1161, 1706, 1941, 2613
BseNI	ACTGG	8 cut(s)	530, 1449, 1502, 1674, 1762, 1806, 2261, 2782
BseRI	GAGGAG	1 cut(s)	1101
BseXI	GCAGC	4 cut(s)	124, 431, 1810, 2071
Bsh1236I	CGCG	1 cut(s)	1144
Bsh1285I	CGRYCG	1 cut(s)	2531
BshFI	GGCC	7 cut(s)	1141, 1634, 1805, 1953, 2166, 2648, 2791
BsiEI	CGRYCG	1 cut(s)	2531
BsiHKCI	CYCGRG	1 cut(s)	126
BsiSI	CCGG	5 cut(s)	479, 537, 721, 1083, 2650
BslFI	GGGAC	3 cut(s)	880, 1571, 2594
BslI	CCNNNNNNNGG	4 cut(s)	131, 169, 536, 2374
BsmAI	GTCTC	2 cut(s)	817, 879
BsmBI	CGTCTC	1 cut(s)	879
BsmFI	GGGAC	3 cut(s)	880, 1571, 2594
BsmI	GAATGC	3 cut(s)	499, 915, 1165
BsnI	GGCC	7 cut(s)	1141, 1634, 1805, 1953, 2166, 2648, 2791
BsoBI	CYCGRG	1 cut(s)	126
Bsp1720I	GCTNAGC	1 cut(s)	177
Bsp19I	CCATGG	3 cut(s)	648, 1459, 2161
BspACI	CCGC	8 cut(s)	476, 703, 1142, 1423, 1793, 2210, 2531, 2641
BspANI	GGCC	7 cut(s)	1141, 1634, 1805, 1953, 2166, 2648, 2791
BspCNI	CTCAG	8 cut(s)	169, 381, 501, 827, 1162, 1707, 1940, 2612
BspFNI	CGCG	1 cut(s)	1144
BspLI	GGNNCC	6 cut(s)	123, 229, 378, 2158, 2214, 2229
BspOI	GCTAGC	1 cut(s)	1154
BspPI	GGATC	5 cut(s)	68, 371, 384, 2151, 2164
BspQI	GCTCTTC	1 cut(s)	602
BspTI	CTTAAG	1 cut(s)	2012
BsrDI	GCAATG	1 cut(s)	1381
BsrFI	RCCGGY	1 cut(s)	478
BsrI	ACTGG	8 cut(s)	530, 1449, 1502, 1674, 1762, 1806, 2261, 2782
BssAI	RCCGGY	1 cut(s)	478
BssECI	CCNNGG	7 cut(s)	648, 978, 1397, 1459, 2161, 2611, 2612
BssNAI	GTATAC	1 cut(s)	746
BssT1I	CCWWGG	4 cut(s)	648, 1397, 1459, 2161
Bst1107I	GTATAC	1 cut(s)	746
Bst2UI	CCWGG	7 cut(s)	78, 620, 980, 1206, 1355, 2348, 2613
Bst6I	CTCTTC	6 cut(s)	42, 483, 602, 620, 1991, 2648
BstAFI	CTTAAG	1 cut(s)	2012
BstBAI	YACGTR	1 cut(s)	1475
BstC8I	GCNNGC	7 cut(s)	302, 1033, 1152, 1427, 1724, 2188, 2192
BstDSI	CCRYGG	3 cut(s)	648, 1459, 2161
BstENI	CCTNNNNNAGG	1 cut(s)	2372
BstF5I	GGATG	6 cut(s)	1166, 1363, 1846, 1980, 2354, 2358
BstFNI	CGCG	1 cut(s)	1144
BstHHI	GCGC	1 cut(s)	2313
BstMAI	GTCTC	2 cut(s)	817, 879
BstMCI	CGRYCG	1 cut(s)	2531
BstNI	CCWGG	7 cut(s)	78, 620, 980, 1206, 1355, 2348, 2613
BstNSI	RCATGY	3 cut(s)	1429, 2317, 2471
BstSCI	CCNGG	9 cut(s)	76, 535, 618, 978, 1204, 1353, 2346, 2611, 2648
BstSFI	CTRYAG	3 cut(s)	936, 1935, 2052
BstUI	CGCG	1 cut(s)	1144
BstV1I	GCAGC	4 cut(s)	124, 431, 1810, 2071
BstV2I	GAAGAC	1 cut(s)	1920
BstX2I	RGATCY	3 cut(s)	376, 961, 2156
BstYI	RGATCY	3 cut(s)	376, 961, 2156
BstZ17I	GTATAC	1 cut(s)	746
BsuI	GTATCC	1 cut(s)	901
BsuRI	GGCC	7 cut(s)	1141, 1634, 1805, 1953, 2166, 2648, 2791
BtgI	CCRYGG	3 cut(s)	648, 1459, 2161
BtgZI	GCGATG	1 cut(s)	1284
BtsCI	GGATG	6 cut(s)	1166, 1363, 1846, 1980, 2354, 2358
BtsIMutI	CAGTG	3 cut(s)	1066, 1434, 2404
Cac8I	GCNNGC	7 cut(s)	302, 1033, 1152, 1427, 1724, 2188, 2192
CaiI	CAGNNNCTG	1 cut(s)	508
CfoI	GCGC	1 cut(s)	2313
Cfr10I	RCCGGY	1 cut(s)	478
Cfr13I	GGNCC	4 cut(s)	1456, 2165, 2213, 2647
CseI	GACGC	1 cut(s)	182
CsiI	ACCWGGT	1 cut(s)	618
Csp6I	GTAC	3 cut(s)	257, 1899, 2487
CviQI	GTAC	3 cut(s)	257, 1899, 2487
DraIII	CACNNNGTG	1 cut(s)	771
EaeI	YGGCCR	4 cut(s)	1139, 1632, 1803, 2789
Eam1104I	CTCTTC	6 cut(s)	42, 483, 602, 620, 1991, 2648
EarI	CTCTTC	6 cut(s)	42, 483, 602, 620, 1991, 2648
Eco130I	CCWWGG	4 cut(s)	648, 1397, 1459, 2161
Eco32I	GATATC	1 cut(s)	1120
Eco47I	GGWCC	2 cut(s)	1456, 2213
Eco57I	CTGAAG	6 cut(s)	588, 1664, 1797, 2271, 2481, 2691
Eco72I	CACGTG	1 cut(s)	1475
Eco88I	CYCGRG	1 cut(s)	126
EcoNI	CCTNNNNNAGG	1 cut(s)	2372
EcoO109I	RGGNCCY	1 cut(s)	2213
EcoRI	GAATTC	1 cut(s)	2173
EcoRII	CCWGG	7 cut(s)	76, 618, 978, 1204, 1353, 2346, 2611
EcoRV	GATATC	1 cut(s)	1120
EcoT14I	CCWWGG	4 cut(s)	648, 1397, 1459, 2161
EcoT22I	ATGCAT	1 cut(s)	142
ErhI	CCWWGG	4 cut(s)	648, 1397, 1459, 2161
Esp3I	CGTCTC	1 cut(s)	879
FalI	AAGNNNNNCTT	4 cut(s)	538, 570, 1314, 1346
FaqI	GGGAC	3 cut(s)	880, 1571, 2594
FauI	CCCGC	2 cut(s)	2203, 2648
FbaI	TGATCA	1 cut(s)	2770
FblI	GTMKAC	3 cut(s)	745, 2436, 2475
Fnu4HI	GCNGC	6 cut(s)	113, 420, 476, 1142, 1824, 2085
FokI	GGATG	6 cut(s)	1173, 1370, 1853, 1987, 2341, 2345
Fsp4HI	GCNGC	6 cut(s)	113, 420, 476, 1142, 1824, 2085
GlaI	GCGC	1 cut(s)	2312
GluI	GCNGC	6 cut(s)	113, 420, 476, 1142, 1824, 2085
GsuI	CTGGAG	1 cut(s)	2799
HaeIII	GGCC	7 cut(s)	1141, 1634, 1805, 1953, 2166, 2648, 2791
HapII	CCGG	5 cut(s)	479, 537, 721, 1083, 2650
HgaI	GACGC	1 cut(s)	182
HhaI	GCGC	1 cut(s)	2313
Hin6I	GCGC	1 cut(s)	2311
HinP1I	GCGC	1 cut(s)	2311
HincII	GTYRAC	2 cut(s)	1755, 2119
HindII	GTYRAC	2 cut(s)	1755, 2119
HindIII	AAGCTT	1 cut(s)	188
HpaII	CCGG	5 cut(s)	479, 537, 721, 1083, 2650
HphI	GGTGA	7 cut(s)	146, 634, 1331, 1491, 1856, 2318, 2734
Hpy166II	GTNNAC	8 cut(s)	746, 1434, 1659, 1755, 2119, 2437, 2476, 2725
Hpy8I	GTNNAC	8 cut(s)	746, 1434, 1659, 1755, 2119, 2437, 2476, 2725
HpyAV	CCTTC	7 cut(s)	168, 367, 904, 1547, 1943, 2246, 2723
HpyCH4IV	ACGT	2 cut(s)	872, 1474
HpySE526I	ACGT	2 cut(s)	872, 1474
HspAI	GCGC	1 cut(s)	2311
Ksp22I	TGATCA	1 cut(s)	2770
LguI	GCTCTTC	1 cut(s)	602
LmnI	GCTCC	5 cut(s)	127, 784, 1003, 1463, 2227
Lsp1109I	GCAGC	4 cut(s)	124, 431, 1810, 2071
LweI	GCATC	8 cut(s)	51, 127, 553, 685, 1298, 1709, 1810, 2173
MabI	ACCWGGT	1 cut(s)	618
MaeII	ACGT	2 cut(s)	872, 1474
MaeIII	GTNAC	4 cut(s)	845, 1405, 1705, 2665
MfeI	CAATTG	5 cut(s)	261, 1534, 1610, 1662, 2235
MflI	RGATCY	3 cut(s)	376, 961, 2156
MlsI	TGGCCA	3 cut(s)	1634, 1805, 2791
MluNI	TGGCCA	3 cut(s)	1634, 1805, 2791
MlyI	GAGTC	2 cut(s)	1553, 2667
MmeI	TCCRAC	2 cut(s)	417, 2003
Mox20I	TGGCCA	3 cut(s)	1634, 1805, 2791
Mph1103I	ATGCAT	1 cut(s)	142
MroXI	GAANNNNTTC	6 cut(s)	346, 629, 761, 1305, 1332, 1856
MscI	TGGCCA	3 cut(s)	1634, 1805, 2791
MseI	TTAA	7 cut(s)	318, 336, 899, 1071, 1577, 2013, 2580
MslI	CAYNNNNRTG	2 cut(s)	1590, 2411
Msp20I	TGGCCA	3 cut(s)	1634, 1805, 2791
MspCI	CTTAAG	1 cut(s)	2012
MspI	CCGG	5 cut(s)	479, 537, 721, 1083, 2650
MspR9I	CCNGG	9 cut(s)	78, 537, 620, 980, 1206, 1355, 2348, 2613, 2650
MunI	CAATTG	5 cut(s)	261, 1534, 1610, 1662, 2235
Mva1269I	GAATGC	3 cut(s)	499, 915, 1165
MvaI	CCWGG	7 cut(s)	78, 620, 980, 1206, 1355, 2348, 2613
MvnI	CGCG	1 cut(s)	1144
NciI	CCSGG	2 cut(s)	537, 2650
NcoI	CCATGG	3 cut(s)	648, 1459, 2161
NheI	GCTAGC	1 cut(s)	1150
NlaIV	GGNNCC	6 cut(s)	123, 229, 378, 2158, 2214, 2229
NsiI	ATGCAT	1 cut(s)	142
NspI	RCATGY	3 cut(s)	1429, 2317, 2471
PaeI	GCATGC	1 cut(s)	1429
PasI	CCCWGGG	1 cut(s)	2612
PciI	ACATGT	1 cut(s)	2467
PciSI	GCTCTTC	1 cut(s)	602
PctI	GAATGC	3 cut(s)	499, 915, 1165
PdmI	GAANNNNTTC	6 cut(s)	346, 629, 761, 1305, 1332, 1856
PfeI	GAWTC	9 cut(s)	11, 308, 452, 630, 821, 1259, 1931, 2335, 2351
PflFI	GACNNNGTC	1 cut(s)	871
PkrI	GCNGC	6 cut(s)	114, 421, 477, 1143, 1825, 2086
PleI	GAGTC	2 cut(s)	1553, 2666
PmaCI	CACGTG	1 cut(s)	1475
PmlI	CACGTG	1 cut(s)	1475
PpsI	GAGTC	2 cut(s)	1553, 2666
Ppu21I	YACGTR	1 cut(s)	1475
PpuMI	RGGWCCY	1 cut(s)	2213
PscI	ACATGT	1 cut(s)	2467
Psp5II	RGGWCCY	1 cut(s)	2213
Psp6I	CCWGG	7 cut(s)	76, 618, 978, 1204, 1353, 2346, 2611
PspCI	CACGTG	1 cut(s)	1475
PspGI	CCWGG	7 cut(s)	76, 618, 978, 1204, 1353, 2346, 2611
PspN4I	GGNNCC	6 cut(s)	123, 229, 378, 2158, 2214, 2229
PspPI	GGNCC	4 cut(s)	1456, 2165, 2213, 2647
PspPPI	RGGWCCY	1 cut(s)	2213
PstNI	CAGNNNCTG	1 cut(s)	508
PsuI	RGATCY	3 cut(s)	376, 961, 2156
PsyI	GACNNNGTC	1 cut(s)	871
RsaI	GTAC	3 cut(s)	258, 1900, 2488
RsaNI	GTAC	3 cut(s)	257, 1899, 2487
RseI	CAYNNNNRTG	2 cut(s)	1590, 2411
SapI	GCTCTTC	1 cut(s)	602
SaqAI	TTAA	7 cut(s)	318, 336, 899, 1071, 1577, 2013, 2580
SatI	GCNGC	6 cut(s)	113, 420, 476, 1142, 1824, 2085
Sau96I	GGNCC	4 cut(s)	1456, 2165, 2213, 2647
SchI	GAGTC	2 cut(s)	1553, 2667
ScrFI	CCNGG	9 cut(s)	78, 537, 620, 980, 1206, 1355, 2348, 2613, 2650
SexAI	ACCWGGT	1 cut(s)	618
SfaNI	GCATC	8 cut(s)	51, 127, 553, 685, 1298, 1709, 1810, 2173
SfcI	CTRYAG	3 cut(s)	936, 1935, 2052
SgrAI	CRCCGGYG	1 cut(s)	478
SinI	GGWCC	2 cut(s)	1456, 2213
SmiMI	CAYNNNNRTG	2 cut(s)	1590, 2411
SmlI	CTYRAG	2 cut(s)	2012, 2130
SmoI	CTYRAG	2 cut(s)	2012, 2130
SphI	GCATGC	1 cut(s)	1429
SsiI	CCGC	8 cut(s)	476, 703, 1142, 1423, 1793, 2210, 2531, 2641
SspI	AATATT	2 cut(s)	469, 1076
StyD4I	CCNGG	9 cut(s)	76, 535, 618, 978, 1204, 1353, 2346, 2611, 2648
StyI	CCWWGG	4 cut(s)	648, 1397, 1459, 2161
TaiI	ACGT	2 cut(s)	875, 1477
TaqI	TCGA	2 cut(s)	45, 1668
TauI	GCSGC	2 cut(s)	478, 1144
TfiI	GAWTC	9 cut(s)	11, 308, 452, 630, 821, 1259, 1931, 2335, 2351
Tru1I	TTAA	7 cut(s)	318, 336, 899, 1071, 1577, 2013, 2580
Tru9I	TTAA	7 cut(s)	318, 336, 899, 1071, 1577, 2013, 2580
TscAI	CASTG	3 cut(s)	1066, 1441, 2404
TseI	GCWGC	4 cut(s)	112, 419, 1823, 2084
TspDTI	ATGAA	6 cut(s)	531, 622, 1566, 1602, 1673, 1824
TspGWI	ACGGA	1 cut(s)	2768
TspRI	CASTG	3 cut(s)	1066, 1441, 2404
Tth111I	GACNNNGTC	1 cut(s)	871
Vha464I	CTTAAG	1 cut(s)	2012
VpaK11BI	GGWCC	2 cut(s)	1456, 2213
XagI	CCTNNNNNAGG	1 cut(s)	2372
XapI	RAATTY	4 cut(s)	759, 1783, 2047, 2173
XbaI	TCTAGA	2 cut(s)	877, 2179
XceI	RCATGY	3 cut(s)	1429, 2317, 2471
XmiI	GTMKAC	3 cut(s)	745, 2436, 2475
XmnI	GAANNNNTTC	6 cut(s)	346, 629, 761, 1305, 1332, 1856
Zsp2I	ATGCAT	1 cut(s)	142

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.