MD12G1236500.v1.1

Mediator-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0017895
Physical Location & Seq
Forward (+)
31041172 .. 31042473
1302 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1236500.v1.1.491

Sequence Viewer

Length: 1302 bp
ATGGCCCCCGATCAGGACGACGCCGTTCTCCCCGAAGGCGACCTCTCCTCCTCCTCCCAGGACGACGAAGAAGAAGAAGACGACGTCGTCGACGAAGACGAGGAAGAAGAAGACGTCGAAGACGATGATGACGTTTTGAACGACGATGTCATCAACTCCGCCTCCCCTTCCACGTCATCCGCCGGCGCCACGGATTCTATCCCCGTCGCCATCGCCACCGCCACTCCAACCGTCACCGTCGCCCTTCCCGCTCGGGCCCCGCCTACTGTGCCTCTGACCCTCACGTCTGCTCCAACCACCGTAATCGCAAATGACGCCGTCTTGTCATCCGATCCCAAACGGCACCCCGCGCTGTCGTCCCAGCCGGAGGAGAAAAAACCGGCGGCGCTGGACGATTCTCGGAGACTCTTCCAGAGGCTGTGGACCGACGAGGACGAGATCGAGCTGTTACAGGGCTTCCTCGACTACACGACGCAGCGAGGCAGCAGAGGCTCGCATGGCCAAAACGACACCGCTTTGTTCTACGACCAGATCAAGTCCAAGCTCCAGCTCGACTTCAACAAGAACCAGCTCGTCGAGAAGCTCCGGCGGCTGAAGAAGAAGTACCGGAACGTCCTCAGCAAAATCGCGAGCGGCAAAGACCTCAGCTTCAAGAGCCCTCATGATCAGGCGACGTTCGAGATCTCCCGCAAGATCTGGAGCAATATCGGCCGAATTGGCGCCGACGAGAACGCCCTCGACGACGAAGATCCCCCTAACAATCCTAACTTCAGCAGTTGTGAGATCAAGGCCGAGGAAGCTGCCGGTTTCGCCGGTGATAAGAAGTCCACGCCGAGATCGCGCAAGCGCTCGCGAATACAGCCAAGGGCCGACGAGAAACGGCCGCCTCCGCCGCTACGTGGCGGTTTCGTTGAGCCGTCGCCGGTAATTAAAGATAACAACAACAGCATTAATATTAGTAACAATGGTGGTGCCGGTGGTGGTAACAATTGTAACAATTGTAATGTTCAAGGGTTGATTGAGGAGACGGTGAAGAGTTGTTTGTCTCCATTGTTCAAGGAGTTGCTGAGTAATGCAATGGGTGGCGGGCCGTCTAGAGGCTTCGGAGGTTTGGCATTCAATCCGATTCCCTTGAGCTTCGGTGGGCTTCCGATGAACTTGAATTTCGGGGGCGGAGAAGTGGCGGATGACAAGTGGAGGAAGCAGCAGATTTTGGAGCTGGAGGTGTACTCGAAGCGGCTGGACTTGGTTCAGAATCAGATCAAAGTGGCATTGGACGAGTTGCGGTCCAATGGCGGGTGA

Protein Analysis

434

Amino Acids

47.04

Weight (kDa)

4.64

Isoelectric Point (pI)

59.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GeBP-like_DBD PF04504 137 - 234 2e-32 Glabrous-enhancer-binding protein-like family, DBD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017895)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04930 AT3G04930 AT5G28040 AT5G28040
fragaria_vesca FvH4_6g03660
malus_domestica MD04G1219700.v1.1 MD12G1236500.v1.1
prunus_persica Prupe.6G338800_v2.0.a1 Prupe.6G338800_v2.0.a1
pyrus_communis pycom04g19470
rosa_samantha Rh6BG252100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 86, 283
AatII GACGTC 2 cut(s) 87, 117
AccB1I GGYRCC 4 cut(s) 185, 342, 719, 971
AccBSI CCGCTC 2 cut(s) 251, 633
AccI GTMKAC 1 cut(s) 90
AccII CGCG 4 cut(s) 350, 629, 841, 853
AclWI GGATC 2 cut(s) 326, 743
AcoI YGGCCR 3 cut(s) 499, 709, 881
AcsI RAATTY 1 cut(s) 1162
AcuI CTGAAG 2 cut(s) 614, 754
AcyI GRCGYC 6 cut(s) 21, 84, 114, 186, 315, 720
AfaI GTAC 2 cut(s) 605, 1229
AfeI AGCGCT 1 cut(s) 848
AfiI CCNNNNNNNGG 5 cut(s) 13, 367, 899, 1097, 1296
AgsI TTSAA 7 cut(s) 139, 559, 652, 1010, 1057, 1120, 1162
AjiI CACGTC 2 cut(s) 174, 285
AjnI CCWGG 1 cut(s) 57
AluBI AGCT 9 cut(s) 445, 544, 550, 571, 583, 648, 800, 1137, 1219
AluI AGCT 9 cut(s) 445, 544, 550, 571, 583, 648, 800, 1137, 1219
Alw26I GTCTC 3 cut(s) 397, 1019, 1050
AlwI GGATC 2 cut(s) 326, 743
AlwNI CAGNNNCTG 1 cut(s) 418
Ama87I CYCGRG 1 cut(s) 252
Aor51HI AGCGCT 1 cut(s) 848
AoxI GGCC 8 cut(s) 3, 255, 499, 709, 789, 867, 881, 1088
ApaI GGGCCC 1 cut(s) 259
ApeKI GCWGC 4 cut(s) 475, 483, 800, 1204
ApoI RAATTY 1 cut(s) 1162
AseI ATTAAT 1 cut(s) 951
AspLEI GCGC 6 cut(s) 188, 352, 388, 722, 843, 849
AspS9I GGNCC 7 cut(s) 4, 255, 256, 423, 867, 1088, 1287
AsuHPI GGTGA 3 cut(s) 226, 827, 1042
AvaI CYCGRG 1 cut(s) 252
AvaII GGWCC 2 cut(s) 423, 1287
BaeGI GKGCMC 1 cut(s) 259
BalI TGGCCA 1 cut(s) 501
BanI GGYRCC 4 cut(s) 185, 342, 719, 971
BanII GRGCYC 2 cut(s) 259, 659
BarI GAAGNNNNNNTAC 2 cut(s) 587, 619
BbsI GAAGAC 4 cut(s) 84, 102, 117, 126
BbvCI CCTCAGC 2 cut(s) 617, 644
BbvI GCAGC 4 cut(s) 487, 495, 787, 1216
BccI CCATC 1 cut(s) 218
BceAI ACGGC 6 cut(s) 8, 302, 356, 896, 901, 1075
BcgI CGANNNNNNTGC 2 cut(s) 782, 816
BciT130I CCWGG 1 cut(s) 59
BclI TGATCA 1 cut(s) 664
BcoDI GTCTC 3 cut(s) 397, 1019, 1050
BfaI CTAG 1 cut(s) 1095
BfoI RGCGCY 4 cut(s) 189, 389, 723, 850
BglI GCCNNNNNGGC 1 cut(s) 717
BglII AGATCT 2 cut(s) 681, 693
Bme1390I CCNGG 1 cut(s) 59
Bme18I GGWCC 2 cut(s) 423, 1287
BmeT110I CYCGRG 1 cut(s) 252
BmgBI CACGTC 2 cut(s) 174, 285
BmgT120I GGNCC 7 cut(s) 4, 255, 256, 423, 867, 1088, 1287
BmiI GGNNCC 7 cut(s) 6, 187, 257, 258, 344, 721, 973
BmrFI CCNGG 1 cut(s) 59
BpiI GAAGAC 4 cut(s) 84, 102, 117, 126
BplI GAGNNNNNCTC 2 cut(s) 1214, 1246
BpmI CTGGAG 3 cut(s) 530, 718, 1241
Bpu10I CCTNAGC 2 cut(s) 617, 644
BpuEI CTTGAG 1 cut(s) 1153
BsaAI YACGTR 1 cut(s) 899
BsaHI GRCGYC 6 cut(s) 21, 84, 114, 186, 315, 720
BsaJI CCNNGG 4 cut(s) 57, 189, 792, 863
BsaWI WCCGGW 1 cut(s) 606
BsaXI ACNNNNNCTCC 8 cut(s) 32, 62, 146, 176, 208, 238, 274, 304
Bsc4I CCNNNNNNNGG 5 cut(s) 13, 367, 899, 1097, 1296
Bse118I RCCGGY 6 cut(s) 182, 379, 803, 812, 922, 974
Bse3DI GCAATG 1 cut(s) 1083
BseBI CCWGG 1 cut(s) 59
BseDI CCNNGG 4 cut(s) 57, 189, 792, 863
BseGI GGATG 3 cut(s) 176, 326, 1192
BseLI CCNNNNNNNGG 5 cut(s) 13, 367, 899, 1097, 1296
BseMI GCAATG 1 cut(s) 1083
BseMII CTCAG 3 cut(s) 631, 658, 1058
BseRI GAGGAG 5 cut(s) 37, 40, 43, 383, 1037
BseSI GKGCMC 1 cut(s) 259
BseX3I CGGCCG 2 cut(s) 709, 881
BseXI GCAGC 4 cut(s) 487, 495, 787, 1216
BseYI CCCAGC 1 cut(s) 360
Bsh1236I CGCG 4 cut(s) 350, 629, 841, 853
Bsh1285I CGRYCG 2 cut(s) 712, 884
BshFI GGCC 8 cut(s) 5, 257, 501, 711, 791, 869, 883, 1090
BshNI GGYRCC 4 cut(s) 185, 342, 719, 971
BsiEI CGRYCG 2 cut(s) 712, 884
BsiHKCI CYCGRG 1 cut(s) 252
BsiSI CCGG 9 cut(s) 183, 365, 380, 586, 607, 804, 813, 923, 975
BslFI GGGAC 1 cut(s) 343
BslI CCNNNNNNNGG 5 cut(s) 13, 367, 899, 1097, 1296
BsmAI GTCTC 3 cut(s) 397, 1019, 1050
BsmBI CGTCTC 1 cut(s) 1019
BsmFI GGGAC 1 cut(s) 343
BsmI GAATGC 1 cut(s) 1115
BsnI GGCC 8 cut(s) 5, 257, 501, 711, 791, 869, 883, 1090
BsoBI CYCGRG 1 cut(s) 252
Bsp120I GGGCCC 1 cut(s) 255
Bsp1286I GDGCHC 2 cut(s) 259, 659
Bsp68I TCGCGA 2 cut(s) 629, 853
BspANI GGCC 8 cut(s) 5, 257, 501, 711, 791, 869, 883, 1090
BspCNI CTCAG 3 cut(s) 630, 657, 1059
BspFNI CGCG 4 cut(s) 350, 629, 841, 853
BspHI TCATGA 1 cut(s) 661
BspLI GGNNCC 7 cut(s) 6, 187, 257, 258, 344, 721, 973
BspPI GGATC 2 cut(s) 326, 743
BspT107I GGYRCC 4 cut(s) 185, 342, 719, 971
BsrBI CCGCTC 2 cut(s) 251, 633
BsrDI GCAATG 1 cut(s) 1083
BsrFI RCCGGY 6 cut(s) 182, 379, 803, 812, 922, 974
BssAI RCCGGY 6 cut(s) 182, 379, 803, 812, 922, 974
BssECI CCNNGG 4 cut(s) 57, 189, 792, 863
BssNI GRCGYC 6 cut(s) 21, 84, 114, 186, 315, 720
BssT1I CCWWGG 1 cut(s) 863
Bst2UI CCWGG 1 cut(s) 59
Bst4CI ACNGT 5 cut(s) 232, 238, 268, 301, 1030
Bst6I CTCTTC 2 cut(s) 413, 1028
BstACI GRCGYC 6 cut(s) 21, 84, 114, 186, 315, 720
BstBAI YACGTR 1 cut(s) 899
BstC8I GCNNGC 6 cut(s) 184, 494, 631, 845, 851, 1088
BstDEI CTNAG 3 cut(s) 617, 644, 1067
BstDSI CCRYGG 1 cut(s) 189
BstF5I GGATG 3 cut(s) 176, 326, 1192
BstFNI CGCG 4 cut(s) 350, 629, 841, 853
BstH2I RGCGCY 4 cut(s) 189, 389, 723, 850
BstHHI GCGC 6 cut(s) 188, 352, 388, 722, 843, 849
BstMAI GTCTC 3 cut(s) 397, 1019, 1050
BstMCI CGRYCG 2 cut(s) 712, 884
BstNI CCWGG 1 cut(s) 59
BstSCI CCNGG 1 cut(s) 57
BstSLI GKGCMC 1 cut(s) 259
BstUI CGCG 4 cut(s) 350, 629, 841, 853
BstV1I GCAGC 4 cut(s) 487, 495, 787, 1216
BstV2I GAAGAC 4 cut(s) 84, 102, 117, 126
BstX2I RGATCY 3 cut(s) 681, 693, 748
BstYI RGATCY 3 cut(s) 681, 693, 748
BstZI CGGCCG 2 cut(s) 709, 881
BsuRI GGCC 8 cut(s) 5, 257, 501, 711, 791, 869, 883, 1090
BtgI CCRYGG 1 cut(s) 189
BtgZI GCGATG 1 cut(s) 196
BtrI CACGTC 2 cut(s) 174, 285
BtsCI GGATG 3 cut(s) 176, 326, 1192
BtuMI TCGCGA 2 cut(s) 629, 853
Cac8I GCNNGC 6 cut(s) 184, 494, 631, 845, 851, 1088
CaiI CAGNNNCTG 1 cut(s) 418
CciI TCATGA 1 cut(s) 661
CfoI GCGC 6 cut(s) 188, 352, 388, 722, 843, 849
Cfr10I RCCGGY 6 cut(s) 182, 379, 803, 812, 922, 974
Cfr13I GGNCC 7 cut(s) 4, 255, 256, 423, 867, 1088, 1287
CseI GACGC 3 cut(s) 29, 323, 481
Csp6I GTAC 2 cut(s) 604, 1228
CviAII CATG 2 cut(s) 497, 662
CviQI GTAC 2 cut(s) 604, 1228
DdeI CTNAG 3 cut(s) 617, 644, 1067
DinI GGCGCC 2 cut(s) 187, 721
DrdI GACNNNNNNGTC 2 cut(s) 86, 283
DseDI GACNNNNNNGTC 2 cut(s) 86, 283
EaeI YGGCCR 3 cut(s) 499, 709, 881
EagI CGGCCG 2 cut(s) 709, 881
Eam1104I CTCTTC 2 cut(s) 413, 1028
EarI CTCTTC 2 cut(s) 413, 1028
EciI GGCGGA 5 cut(s) 148, 169, 879, 1188, 1199
EclXI CGGCCG 2 cut(s) 709, 881
Eco130I CCWWGG 1 cut(s) 863
Eco24I GRGCYC 2 cut(s) 259, 659
Eco47I GGWCC 2 cut(s) 423, 1287
Eco47III AGCGCT 1 cut(s) 848
Eco52I CGGCCG 2 cut(s) 709, 881
Eco57I CTGAAG 2 cut(s) 614, 754
Eco88I CYCGRG 1 cut(s) 252
EcoO109I RGGNCCY 1 cut(s) 256
EcoRII CCWGG 1 cut(s) 57
EcoT14I CCWWGG 1 cut(s) 863
EcoT38I GRGCYC 2 cut(s) 259, 659
EgeI GGCGCC 2 cut(s) 187, 721
EheI GGCGCC 2 cut(s) 187, 721
ErhI CCWWGG 1 cut(s) 863
Esp3I CGTCTC 1 cut(s) 1019
FaeI CATG 2 cut(s) 500, 665
FaiI YATR 2 cut(s) 498, 663
FaqI GGGAC 1 cut(s) 343
FatI CATG 2 cut(s) 496, 661
FauI CCCGC 6 cut(s) 256, 267, 355, 695, 1079, 1289
FbaI TGATCA 1 cut(s) 664
FblI GTMKAC 1 cut(s) 90
FokI GGATG 3 cut(s) 163, 313, 1199
FriOI GRGCYC 2 cut(s) 259, 659
FspBI CTAG 1 cut(s) 1095
GlaI GCGC 6 cut(s) 187, 351, 387, 721, 842, 848
GsaI CCCAGC 1 cut(s) 364
GsuI CTGGAG 3 cut(s) 530, 718, 1241
HaeII RGCGCY 4 cut(s) 189, 389, 723, 850
HaeIII GGCC 8 cut(s) 5, 257, 501, 711, 791, 869, 883, 1090
HapII CCGG 9 cut(s) 183, 365, 380, 586, 607, 804, 813, 923, 975
HgaI GACGC 3 cut(s) 29, 323, 481
HhaI GCGC 6 cut(s) 188, 352, 388, 722, 843, 849
Hin1I GRCGYC 6 cut(s) 21, 84, 114, 186, 315, 720
Hin1II CATG 2 cut(s) 500, 665
Hin6I GCGC 6 cut(s) 186, 350, 386, 720, 841, 847
HinP1I GCGC 6 cut(s) 186, 350, 386, 720, 841, 847
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HinfI GANTC 5 cut(s) 194, 395, 405, 1126, 1255
HpaII CCGG 9 cut(s) 183, 365, 380, 586, 607, 804, 813, 923, 975
HphI GGTGA 3 cut(s) 226, 827, 1042
Hpy166II GTNNAC 4 cut(s) 91, 423, 828, 1228
Hpy188I TCNGA 8 cut(s) 276, 331, 402, 1106, 1125, 1152, 1254, 1260
Hpy8I GTNNAC 4 cut(s) 91, 423, 828, 1228
HpyAV CCTTC 3 cut(s) 29, 177, 254
HpyCH4III ACNGT 5 cut(s) 232, 238, 268, 301, 1030
HpyCH4IV ACGT 8 cut(s) 84, 114, 132, 173, 284, 612, 674, 898
HpyCH4V TGCA 1 cut(s) 1076
HpyF3I CTNAG 3 cut(s) 617, 644, 1067
HpySE526I ACGT 8 cut(s) 84, 114, 132, 173, 284, 612, 674, 898
Hsp92I GRCGYC 6 cut(s) 21, 84, 114, 186, 315, 720
Hsp92II CATG 2 cut(s) 500, 665
HspAI GCGC 6 cut(s) 186, 350, 386, 720, 841, 847
KasI GGCGCC 2 cut(s) 185, 719
KroI GCCGGC 1 cut(s) 182
KroNI GCCGGC 1 cut(s) 184
Ksp22I TGATCA 1 cut(s) 664
LmnI GCTCC 5 cut(s) 295, 549, 588, 699, 1216
Lsp1109I GCAGC 4 cut(s) 487, 495, 787, 1216
MaeI CTAG 1 cut(s) 1095
MaeII ACGT 8 cut(s) 84, 114, 132, 173, 284, 612, 674, 898
MaeIII GTNAC 5 cut(s) 232, 447, 959, 983, 992
MbiI CCGCTC 2 cut(s) 251, 633
MfeI CAATTG 2 cut(s) 988, 997
MflI RGATCY 3 cut(s) 681, 693, 748
MhlI GDGCHC 2 cut(s) 259, 659
MlsI TGGCCA 1 cut(s) 501
MluCI AATT 5 cut(s) 714, 927, 988, 997, 1162
MluNI TGGCCA 1 cut(s) 501
Mly113I GGCGCC 2 cut(s) 186, 720
MlyI GAGTC 1 cut(s) 399
MmeI TCCRAC 2 cut(s) 251, 317
Mox20I TGGCCA 1 cut(s) 501
MreI CGCCGGCG 1 cut(s) 182
MroNI GCCGGC 1 cut(s) 182
MscI TGGCCA 1 cut(s) 501
MseI TTAA 2 cut(s) 930, 951
Msp20I TGGCCA 1 cut(s) 501
MspI CCGG 9 cut(s) 183, 365, 380, 586, 607, 804, 813, 923, 975
MspR9I CCNGG 1 cut(s) 59
MunI CAATTG 2 cut(s) 988, 997
Mva1269I GAATGC 1 cut(s) 1115
MvaI CCWGG 1 cut(s) 59
MvnI CGCG 4 cut(s) 350, 629, 841, 853
NaeI GCCGGC 1 cut(s) 184
NarI GGCGCC 2 cut(s) 186, 720
NgoMIV GCCGGC 1 cut(s) 182
NlaIII CATG 2 cut(s) 500, 665
NlaIV GGNNCC 7 cut(s) 6, 187, 257, 258, 344, 721, 973
NmeAIII GCCGAG 2 cut(s) 817, 858
NmuCI GTSAC 1 cut(s) 232
NruI TCGCGA 2 cut(s) 629, 853
PagI TCATGA 1 cut(s) 661
PcsI WCGNNNNNNNCGW 7 cut(s) 87, 90, 96, 120, 129, 138, 738
PctI GAATGC 1 cut(s) 1115
PdiI GCCGGC 1 cut(s) 184
PfeI GAWTC 4 cut(s) 194, 395, 1126, 1255
PflFI GACNNNGTC 4 cut(s) 83, 86, 146, 317
PleI GAGTC 1 cut(s) 399
PluTI GGCGCC 2 cut(s) 189, 723
PpsI GAGTC 1 cut(s) 399
Ppu21I YACGTR 1 cut(s) 899
PshBI ATTAAT 1 cut(s) 951
Psp6I CCWGG 1 cut(s) 57
PspFI CCCAGC 1 cut(s) 360
PspGI CCWGG 1 cut(s) 57
PspN4I GGNNCC 7 cut(s) 6, 187, 257, 258, 344, 721, 973
PspOMI GGGCCC 1 cut(s) 255
PspPI GGNCC 7 cut(s) 4, 255, 256, 423, 867, 1088, 1287
PstNI CAGNNNCTG 1 cut(s) 418
PsuI RGATCY 3 cut(s) 681, 693, 748
PsyI GACNNNGTC 4 cut(s) 83, 86, 146, 317
RruI TCGCGA 2 cut(s) 629, 853
RsaI GTAC 2 cut(s) 605, 1229
RsaNI GTAC 2 cut(s) 604, 1228
SalI GTCGAC 1 cut(s) 89
SaqAI TTAA 2 cut(s) 930, 951
Sau96I GGNCC 7 cut(s) 4, 255, 256, 423, 867, 1088, 1287
SchI GAGTC 1 cut(s) 399
ScrFI CCNGG 1 cut(s) 59
SduI GDGCHC 2 cut(s) 259, 659
SfoI GGCGCC 2 cut(s) 187, 721
SgrAI CRCCGGYG 2 cut(s) 182, 812
SgrDI CGTCGACG 1 cut(s) 89
SinI GGWCC 2 cut(s) 423, 1287
SmlI CTYRAG 1 cut(s) 1132
SmoI CTYRAG 1 cut(s) 1132
Sse9I AATT 5 cut(s) 714, 927, 988, 997, 1162
SspDI GGCGCC 2 cut(s) 185, 719
SspI AATATT 1 cut(s) 955
SspMI CTAG 1 cut(s) 1095
StyD4I CCNGG 1 cut(s) 57
StyI CCWWGG 1 cut(s) 863
TaaI ACNGT 5 cut(s) 232, 238, 268, 301, 1030
TaiI ACGT 8 cut(s) 87, 117, 135, 176, 287, 615, 677, 901
TaqI TCGA 9 cut(s) 90, 117, 441, 462, 552, 576, 678, 738, 1232
TaqII GACCGA 1 cut(s) 440
TasI AATT 5 cut(s) 714, 927, 988, 997, 1162
TatI WGTACW 1 cut(s) 1227
TauI GCSGC 6 cut(s) 386, 592, 636, 886, 895, 1240
TfiI GAWTC 4 cut(s) 194, 395, 1126, 1255
Tru1I TTAA 2 cut(s) 930, 951
Tru9I TTAA 2 cut(s) 930, 951
TseFI GTSAC 1 cut(s) 232
TseI GCWGC 4 cut(s) 475, 483, 800, 1204
Tsp45I GTSAC 1 cut(s) 232
TspDTI ATGAA 1 cut(s) 1169
TspGWI ACGGA 1 cut(s) 206
Tth111I GACNNNGTC 4 cut(s) 83, 86, 146, 317
VpaK11BI GGWCC 2 cut(s) 423, 1287
VspI ATTAAT 1 cut(s) 951
XapI RAATTY 1 cut(s) 1162
XbaI TCTAGA 1 cut(s) 1094
XmiI GTMKAC 1 cut(s) 90
XspI CTAG 1 cut(s) 1095
ZraI GACGTC 2 cut(s) 85, 115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.