Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0007747

Physical Location & Seq

Genomic Coordinates Reverse (-)

31091558 .. 31095899

Gene Structure 4342 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1237300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1716 bp

ATGGCTTCTTGGGCTCTTTTGCCGGCAGCTCCAGTAACCGGTCGCTGTATAGTGACTCCTGCCAGGACAAAGCGGTCCTCCTCCTTCTGGGTTCGTTCTTCTCTGGACACAAATGTTTCTGACATGGGGGTTAACGCTCCAAAAGGGTTGTTTCCACCTGAACCGGAGCGCTACCGAGGTCCAAAGCTGAAAGTGGCCATTATCGGAGCTGGACTCGCGGGCATGTCAACCGCGGTTGAGCTTTTGGATCAAGGCCATGAGGTGGATATTTATGAGTCGAGGCCCTTCATTGGTGGCAAAGTGGGCTCTTTTGTTGATAAAAAAGGGAACCACATTGAAATGGGACTCCATGTTTTCTTTGGTTGCTACAGTAATCTTTTCCGATTAATGAAAAAGGTGGGTGCAGACGAAAATCTTCTTGTCAAGGATCATACTCACACTTTTGTAAACAAAGGGGGTAGCATTGGTGAACTTGATTTTCGGTTCCCAATTGGAGCACCAATACATGGGATTCTTGCATTTTTGTCTACTAATCAGATTAAGACTTACGATAAAGCAAGAAATGCAGTGGCTCTTGCCTTAAGTCCGGTTGTAAAGGCTCTTGTTAATCCAGATGGAGCGTTGCAGGATGTCCGGAATTTGGATAGTATAAGTTTCTCTGATTGGTTCTTGTCAAAAGGTGGCACGCGAATGAGTATCCAGCGGATGTGGGATCCTGTTGCATATGCACTTGGATTTATTGACTGTGATAATATTAGTGCTCGTTGTATGCTCACTATATTCACATTGTTTGCCACTAAGACCGAGGCTTCCCTTCTACGCATGCTCAAGGGTTCGCCAGATGTCTACTTAAGTGGCCCCATCAGAGATTATATCATTGCAAAGGGGGGCAGGTTTCATCTTAGGTGGGGGTGTAGAGAAATACTGTATGATAAATCTTCTGATGGAGAAACATATGTTGCCGGATTTTCGATGTCTAAGGCTACAAATAAGAAAGTTGTGAAAGCTGACGCTTATGTTGCAGCGTGTGATGTGCCTGGAATCAAGAGATTGCTTCCTTCTCAGTGGAGAGAATTGGATTTTTTCAATAATATTTATGAGCTAGTTGGAGTTCCTGTTGTCACTGTGCAACTTAGATACGACGGTTGGGTCACAGAGTTACAAGATCTAGAACGGTCAAGGAAATCGAAGCAAGCTTTGGGATTAGATAATCTCTTATATACTCCCGATGCAGATTTCTCTTGCTTTGCCGACCTAGCGCTTACTTCTCCTGAAGATTACTACATTGAGGGACAAGGTTCACTCCTCCAATGCGTTTTGACACCAGGTGATCCTTACATGCCTTTACCAAACGAAGAAATTATAGCAAGAGTGACGAAACAGGTTTTGGCTTTATTCCCATCATCCCAAGGTTTGGAAGTCACTTGGTCATCAGTTGTCAAAATTGGGCAATCTCTCTATCGGGAGGGACCTGGCAAAGATCCGTTTAGACCTGATCAGAAGACACCCGTGAAGAATTTCTTCCTTGCTGGCTCATACACAAAACAGGACTATATCGACAGCATGGAAGGGGCAACTTTGTCCGGCAGGCAAGCTTCTGCGTATATATGTGATGCCGGGGAAGAGTTGGTGGGATTGCGAAAGAAGTTTGCAGCCCAAGATTCTGGGGAATACACGAAAGCTGTTAATACTACCGATGAGCTGAGTCTTGTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006629 GO:0006720 GO:0006721 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009507 GO:0009509 GO:0009526 GO:0009536 GO:0009941 GO:0009987 GO:0016108 GO:0016109 GO:0016114 GO:0016116 GO:0016117 GO:0016119 GO:0016120 GO:0016491 GO:0016627 GO:0016635 GO:0031967 GO:0031975 GO:0042214 GO:0042440 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046148 GO:0046246 GO:0052886 GO:0052889 GO:0055114 GO:0071704 GO:1901175 GO:1901177 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00906 ko01100 ko01110 map00906 map01100 map01110

Pfam Domains

Protein Families

Amino_oxidase

Protein Analysis

572

Amino Acids

62.86

Weight (kDa)

7.03

Isoelectric Point (pI)

42.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DAO	PF01266	64 - 99	6.3e-06	FAD dependent oxidoreductase
NAD_binding_8	PF13450	67 - 132	6.8e-14	NAD(P)-binding Rossmann-like domain
Amino_oxidase	PF01593	72 - 536	3.4e-74	Flavin containing amine oxidoreductase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007747)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04870 AT3G04870 AT3G04870 AT3G04870
fragaria_vesca	FvH4_6g03420 FvH4_6g03420
malus_domestica	MD04G1220900.v1.1 MD12G1237300.v1.1
prunus_persica	Prupe.6G340000_v2.0.a1 Prupe.6G340000_v2.0.a1
pyrus_communis	pycom04g19540 pycom12g21820
rosa_chinensis	RchiOBHm_Chr3g0451921
rosa_laevigata	RLG00000025638
rosa_multiflora	Rmu_sc0002901.1_g000003
rosa_roxburghii	Rroxscaffold_164G00436190 Rroxscaffold_6G00429090
rosa_rugosa	Rorug02G0637300 Rorug02G0637400
rosa_samantha	Rh3AG039100 Rh3BG040400 Rh3CG038900 Rh3DG039600
rosa_wichuraiana	Rw3G002920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	73, 1148
Acc36I	ACCTGC	1 cut(s)	882
AccB7I	CCANNNNNTGG	3 cut(s)	262, 506, 1414
AccI	GTMKAC	2 cut(s)	527, 846
AccII	CGCG	3 cut(s)	218, 233, 688
AccIII	TCCGGA	1 cut(s)	633
AciI	CCGC	5 cut(s)	73, 218, 231, 233, 703
AclWI	GGATC	6 cut(s)	255, 435, 707, 720, 1325, 1475
AcoI	YGGCCR	1 cut(s)	195
AcsI	RAATTY	2 cut(s)	637, 1516
AcuI	CTGAAG	1 cut(s)	1293
AdeI	CACNNNGTG	1 cut(s)	1328
AfeI	AGCGCT	2 cut(s)	170, 1260
AfiI	CCNNNNNNNGG	7 cut(s)	38, 87, 262, 290, 506, 640, 1414
AflII	CTTAAG	2 cut(s)	580, 850
AgeI	ACCGGT	1 cut(s)	38
AgsI	TTSAA	2 cut(s)	338, 1087
AjnI	CCWGG	4 cut(s)	62, 1036, 1324, 1471
AjuI	GAANNNNNNNTTGG	4 cut(s)	1181, 1213, 1370, 1402
Alw21I	GWGCWC	2 cut(s)	499, 763
AlwI	GGATC	6 cut(s)	255, 435, 707, 720, 1325, 1475
Aor13HI	TCCGGA	1 cut(s)	633
Aor51HI	AGCGCT	2 cut(s)	170, 1260
AoxI	GGCC	4 cut(s)	195, 253, 281, 856
ApeKI	GCWGC	3 cut(s)	26, 1022, 1652
ApoI	RAATTY	2 cut(s)	637, 1516
AseI	ATTAAT	1 cut(s)	386
AsiGI	ACCGGT	1 cut(s)	38
Asp700I	GAANNNNTTC	3 cut(s)	414, 1517, 1520
AspLEI	GCGC	2 cut(s)	171, 1261
AspS9I	GGNCC	5 cut(s)	75, 179, 282, 857, 1469
AsuC2I	CCSGG	1 cut(s)	1618
AsuHPI	GGTGA	2 cut(s)	479, 1340
AvaII	GGWCC	3 cut(s)	75, 179, 1469
BaeI	ACNNNNGTAYC	2 cut(s)	1129, 1162
BalI	TGGCCA	1 cut(s)	197
BamHI	GGATCC	1 cut(s)	712
BanII	GRGCYC	2 cut(s)	16, 308
BbsI	GAAGAC	1 cut(s)	1508
Bbv12I	GWGCWC	2 cut(s)	499, 763
BbvI	GCAGC	3 cut(s)	38, 1034, 1664
BccI	CCATC	4 cut(s)	608, 869, 938, 1408
BciT130I	CCWGG	4 cut(s)	64, 1038, 1326, 1473
BciVI	GTATCC	1 cut(s)	707
BclI	TGATCA	1 cut(s)	1495
BcnI	CCSGG	1 cut(s)	1618
BfaI	CTAG	3 cut(s)	1103, 1169, 1256
BfmI	CTRYAG	1 cut(s)	367
BfoI	RGCGCY	2 cut(s)	172, 1262
BfrI	CTTAAG	2 cut(s)	580, 850
BfuAI	ACCTGC	1 cut(s)	882
BfuI	GTATCC	1 cut(s)	707
BglII	AGATCT	1 cut(s)	1165
BisI	GCNGC	3 cut(s)	27, 1023, 1653
BlsI	GCNGC	3 cut(s)	28, 1024, 1654
Bme1390I	CCNGG	5 cut(s)	64, 1038, 1326, 1473, 1618
Bme18I	GGWCC	3 cut(s)	75, 179, 1469
BmgT120I	GGNCC	5 cut(s)	75, 179, 282, 857, 1469
BmiI	GGNNCC	5 cut(s)	329, 485, 714, 859, 1470
BmrFI	CCNGG	5 cut(s)	64, 1038, 1326, 1473, 1618
BmsI	GCATC	2 cut(s)	1219, 1603
BpiI	GAAGAC	1 cut(s)	1508
BplI	GAGNNNNNCTC	2 cut(s)	198, 230
BpmI	CTGGAG	1 cut(s)	15
BpuEI	CTTGAG	1 cut(s)	812
BpuMI	CCSGG	1 cut(s)	1618
BsaJI	CCNNGG	5 cut(s)	175, 231, 804, 1408, 1617
BsaWI	WCCGGW	4 cut(s)	38, 163, 586, 633
BsaXI	ACNNNNNCTCC	2 cut(s)	1101, 1131
Bsc4I	CCNNNNNNNGG	7 cut(s)	38, 87, 262, 290, 506, 640, 1414
Bse118I	RCCGGY	2 cut(s)	22, 38
Bse1I	ACTGG	1 cut(s)	32
Bse3DI	GCAATG	1 cut(s)	876
BseAI	TCCGGA	1 cut(s)	633
BseBI	CCWGG	4 cut(s)	64, 1038, 1326, 1473
BseDI	CCNNGG	5 cut(s)	175, 231, 804, 1408, 1617
BseGI	GGATG	3 cut(s)	634, 711, 1403
BseLI	CCNNNNNNNGG	7 cut(s)	38, 87, 262, 290, 506, 640, 1414
BseMI	GCAATG	1 cut(s)	876
BseMII	CTCAG	2 cut(s)	1076, 1694
BseNI	ACTGG	1 cut(s)	32
BseRI	GAGGAG	2 cut(s)	70, 1295
BseXI	GCAGC	3 cut(s)	38, 1034, 1664
BsgI	GTGCAG	1 cut(s)	423
Bsh1236I	CGCG	3 cut(s)	218, 233, 688
Bsh1285I	CGRYCG	1 cut(s)	43
BshFI	GGCC	4 cut(s)	197, 255, 283, 858
BshTI	ACCGGT	1 cut(s)	38
BsiEI	CGRYCG	1 cut(s)	43
BsiHKAI	GWGCWC	2 cut(s)	499, 763
BsiSI	CCGG	8 cut(s)	23, 39, 164, 587, 634, 963, 1584, 1617
BslFI	GGGAC	3 cut(s)	357, 1305, 1482
BslI	CCNNNNNNNGG	7 cut(s)	38, 87, 262, 290, 506, 640, 1414
BsmFI	GGGAC	3 cut(s)	357, 1305, 1482
BsnI	GGCC	4 cut(s)	197, 255, 283, 858
Bsp1286I	GDGCHC	4 cut(s)	16, 308, 499, 763
Bsp13I	TCCGGA	1 cut(s)	633
Bsp143I	GATC	7 cut(s)	247, 427, 712, 1165, 1330, 1480, 1495
BspACI	CCGC	5 cut(s)	73, 218, 231, 233, 703
BspANI	GGCC	4 cut(s)	197, 255, 283, 858
BspCNI	CTCAG	2 cut(s)	1075, 1695
BspEI	TCCGGA	1 cut(s)	633
BspFNI	CGCG	3 cut(s)	218, 233, 688
BspLI	GGNNCC	5 cut(s)	329, 485, 714, 859, 1470
BspMI	ACCTGC	1 cut(s)	882
BspPI	GGATC	6 cut(s)	255, 435, 707, 720, 1325, 1475
BspTI	CTTAAG	2 cut(s)	580, 850
BsrDI	GCAATG	1 cut(s)	876
BsrFI	RCCGGY	2 cut(s)	22, 38
BsrI	ACTGG	1 cut(s)	32
BssAI	RCCGGY	2 cut(s)	22, 38
BssECI	CCNNGG	5 cut(s)	175, 231, 804, 1408, 1617
BssMI	GATC	7 cut(s)	247, 427, 712, 1165, 1330, 1480, 1495
BssT1I	CCWWGG	1 cut(s)	1408
Bst2UI	CCWGG	4 cut(s)	64, 1038, 1326, 1473
Bst4CI	ACNGT	6 cut(s)	371, 746, 927, 1126, 1145, 1176
Bst6I	CTCTTC	1 cut(s)	1617
BstAFI	CTTAAG	2 cut(s)	580, 850
BstAPI	GCANNNNNTGC	1 cut(s)	563
BstC8I	GCNNGC	8 cut(s)	24, 220, 686, 824, 1194, 1531, 1589, 1593
BstDEI	CTNAG	6 cut(s)	798, 902, 978, 1062, 1133, 1703
BstDSI	CCRYGG	1 cut(s)	231
BstF5I	GGATG	3 cut(s)	634, 711, 1403
BstFNI	CGCG	3 cut(s)	218, 233, 688
BstH2I	RGCGCY	2 cut(s)	172, 1262
BstHHI	GCGC	2 cut(s)	171, 1261
BstKTI	GATC	7 cut(s)	250, 430, 715, 1168, 1333, 1483, 1498
BstMBI	GATC	7 cut(s)	247, 427, 712, 1165, 1330, 1480, 1495
BstMCI	CGRYCG	1 cut(s)	43
BstMWI	GCNNNNNNNGC	6 cut(s)	11, 215, 303, 563, 1019, 1256
BstNI	CCWGG	4 cut(s)	64, 1038, 1326, 1473
BstNSI	RCATGY	3 cut(s)	226, 826, 1342
BstSCI	CCNGG	5 cut(s)	62, 1036, 1324, 1471, 1616
BstSFI	CTRYAG	1 cut(s)	367
BstUI	CGCG	3 cut(s)	218, 233, 688
BstV1I	GCAGC	3 cut(s)	38, 1034, 1664
BstV2I	GAAGAC	1 cut(s)	1508
BstX2I	RGATCY	3 cut(s)	712, 1165, 1480
BstXI	CCANNNNNNTGG	1 cut(s)	1664
BstYI	RGATCY	3 cut(s)	712, 1165, 1480
BsuI	GTATCC	1 cut(s)	707
BsuRI	GGCC	4 cut(s)	197, 255, 283, 858
BtgI	CCRYGG	1 cut(s)	231
BtsCI	GGATG	3 cut(s)	634, 711, 1403
BtsI	GCAGTG	1 cut(s)	573
BtsIMutI	CAGTG	3 cut(s)	573, 1070, 1122
BveI	ACCTGC	1 cut(s)	882
Cac8I	GCNNGC	8 cut(s)	24, 220, 686, 824, 1194, 1531, 1589, 1593
CfoI	GCGC	2 cut(s)	171, 1261
Cfr10I	RCCGGY	2 cut(s)	22, 38
Cfr13I	GGNCC	5 cut(s)	75, 179, 282, 857, 1469
Cfr42I	CCGCGG	1 cut(s)	234
CseI	GACGC	1 cut(s)	1019
CsiI	ACCWGGT	1 cut(s)	1324
CspAI	ACCGGT	1 cut(s)	38
CviAII	CATG	8 cut(s)	124, 223, 257, 350, 506, 823, 1339, 1564
DdeI	CTNAG	6 cut(s)	798, 902, 978, 1062, 1133, 1703
DpnI	GATC	7 cut(s)	249, 429, 714, 1167, 1332, 1482, 1497
DpnII	GATC	7 cut(s)	247, 427, 712, 1165, 1330, 1480, 1495
DraIII	CACNNNGTG	1 cut(s)	1328
DrdI	GACNNNNNNGTC	2 cut(s)	73, 1148
DseDI	GACNNNNNNGTC	2 cut(s)	73, 1148
EaeI	YGGCCR	1 cut(s)	195
Eam1104I	CTCTTC	1 cut(s)	1617
EarI	CTCTTC	1 cut(s)	1617
Eco130I	CCWWGG	1 cut(s)	1408
Eco24I	GRGCYC	2 cut(s)	16, 308
Eco47I	GGWCC	3 cut(s)	75, 179, 1469
Eco47III	AGCGCT	2 cut(s)	170, 1260
Eco57I	CTGAAG	1 cut(s)	1293
EcoO109I	RGGNCCY	2 cut(s)	282, 1469
EcoRII	CCWGG	4 cut(s)	62, 1036, 1324, 1471
EcoT14I	CCWWGG	1 cut(s)	1408
EcoT38I	GRGCYC	2 cut(s)	16, 308
ErhI	CCWWGG	1 cut(s)	1408
FaeI	CATG	8 cut(s)	127, 226, 260, 353, 509, 826, 1342, 1567
FalI	AAGNNNNNCTT	2 cut(s)	1505, 1537
FaqI	GGGAC	3 cut(s)	357, 1305, 1482
FatI	CATG	8 cut(s)	123, 222, 256, 349, 505, 822, 1338, 1563
FauI	CCCGC	1 cut(s)	211
FauNDI	CATATG	2 cut(s)	724, 955
FbaI	TGATCA	1 cut(s)	1495
FblI	GTMKAC	2 cut(s)	527, 846
Fnu4HI	GCNGC	3 cut(s)	27, 1023, 1653
FokI	GGATG	3 cut(s)	641, 718, 1390
FriOI	GRGCYC	2 cut(s)	16, 308
Fsp4HI	GCNGC	3 cut(s)	27, 1023, 1653
FspBI	CTAG	3 cut(s)	1103, 1169, 1256
GlaI	GCGC	2 cut(s)	170, 1260
GluI	GCNGC	3 cut(s)	27, 1023, 1653
GsuI	CTGGAG	1 cut(s)	15
HaeII	RGCGCY	2 cut(s)	172, 1262
HaeIII	GGCC	4 cut(s)	197, 255, 283, 858
HapII	CCGG	8 cut(s)	23, 39, 164, 587, 634, 963, 1584, 1617
HgaI	GACGC	1 cut(s)	1019
HhaI	GCGC	2 cut(s)	171, 1261
Hin1II	CATG	8 cut(s)	127, 226, 260, 353, 509, 826, 1342, 1567
Hin6I	GCGC	2 cut(s)	169, 1259
HinP1I	GCGC	2 cut(s)	169, 1259
HincII	GTYRAC	2 cut(s)	133, 228
HindII	GTYRAC	2 cut(s)	133, 228
HindIII	AAGCTT	2 cut(s)	1194, 1593
HinfI	GANTC	8 cut(s)	55, 213, 275, 345, 511, 1041, 1661, 1705
HpaI	GTTAAC	1 cut(s)	133
HpaII	CCGG	8 cut(s)	23, 39, 164, 587, 634, 963, 1584, 1617
HphI	GGTGA	2 cut(s)	479, 1340
Hpy166II	GTNNAC	7 cut(s)	133, 228, 448, 470, 528, 847, 1301
Hpy188I	TCNGA	8 cut(s)	121, 206, 383, 537, 661, 866, 943, 1500
Hpy188III	TCNNGA	8 cut(s)	104, 611, 634, 1045, 1169, 1226, 1271, 1463
Hpy8I	GTNNAC	7 cut(s)	133, 228, 448, 470, 528, 847, 1301
Hpy99I	CGWCG	1 cut(s)	1145
HpyAV	CCTTC	5 cut(s)	94, 295, 824, 1068, 1562
HpyCH4III	ACNGT	6 cut(s)	371, 746, 927, 1126, 1145, 1176
HpyF10VI	GCNNNNNNNGC	6 cut(s)	11, 215, 303, 563, 1019, 1256
HpyF3I	CTNAG	6 cut(s)	798, 902, 978, 1062, 1133, 1703
Hsp92II	CATG	8 cut(s)	127, 226, 260, 353, 509, 826, 1342, 1567
HspAI	GCGC	2 cut(s)	169, 1259
Kpn2I	TCCGGA	1 cut(s)	633
KroI	GCCGGC	1 cut(s)	22
KroNI	GCCGGC	1 cut(s)	24
Ksp22I	TGATCA	1 cut(s)	1495
KspAI	GTTAAC	1 cut(s)	133
KspI	CCGCGG	1 cut(s)	234
Kzo9I	GATC	7 cut(s)	247, 427, 712, 1165, 1330, 1480, 1495
LmnI	GCTCC	6 cut(s)	34, 142, 166, 206, 494, 617
Lsp1109I	GCAGC	3 cut(s)	38, 1034, 1664
LweI	GCATC	2 cut(s)	1219, 1603
MabI	ACCWGGT	1 cut(s)	1324
MaeI	CTAG	3 cut(s)	1103, 1169, 1256
MaeIII	GTNAC	7 cut(s)	34, 52, 1120, 1150, 1158, 1372, 1420
MalI	GATC	7 cut(s)	249, 429, 714, 1167, 1332, 1482, 1497
MboI	GATC	7 cut(s)	247, 427, 712, 1165, 1330, 1480, 1495
MboII	GAAGA	9 cut(s)	90, 407, 930, 1286, 1367, 1513, 1513, 1525, 1634
MfeI	CAATTG	1 cut(s)	489
MflI	RGATCY	3 cut(s)	712, 1165, 1480
MhlI	GDGCHC	4 cut(s)	16, 308, 499, 763
MlsI	TGGCCA	1 cut(s)	197
MluCI	AATT	6 cut(s)	489, 637, 1073, 1359, 1443, 1516
MluNI	TGGCCA	1 cut(s)	197
MlyI	GAGTC	5 cut(s)	49, 207, 284, 339, 1714
MmeI	TCCRAC	1 cut(s)	1087
MnlI	CCTC	9 cut(s)	88, 91, 170, 253, 273, 799, 1282, 1316, 1459
Mox20I	TGGCCA	1 cut(s)	197
MroI	TCCGGA	1 cut(s)	633
MroNI	GCCGGC	1 cut(s)	22
MroXI	GAANNNNTTC	3 cut(s)	414, 1517, 1520
MscI	TGGCCA	1 cut(s)	197
MseI	TTAA	7 cut(s)	132, 386, 540, 581, 606, 851, 1686
MslI	CAYNNNNRTG	2 cut(s)	338, 689
Msp20I	TGGCCA	1 cut(s)	197
MspA1I	CMGCKG	2 cut(s)	233, 703
MspCI	CTTAAG	2 cut(s)	580, 850
MspI	CCGG	8 cut(s)	23, 39, 164, 587, 634, 963, 1584, 1617
MspR9I	CCNGG	5 cut(s)	64, 1038, 1326, 1473, 1618
MunI	CAATTG	1 cut(s)	489
MvaI	CCWGG	4 cut(s)	64, 1038, 1326, 1473
MvnI	CGCG	3 cut(s)	218, 233, 688
MwoI	GCNNNNNNNGC	6 cut(s)	11, 215, 303, 563, 1019, 1256
NaeI	GCCGGC	1 cut(s)	24
NciI	CCSGG	1 cut(s)	1618
NdeI	CATATG	2 cut(s)	724, 955
NdeII	GATC	7 cut(s)	247, 427, 712, 1165, 1330, 1480, 1495
NgoMIV	GCCGGC	1 cut(s)	22
NlaIII	CATG	8 cut(s)	127, 226, 260, 353, 509, 826, 1342, 1567
NlaIV	GGNNCC	5 cut(s)	329, 485, 714, 859, 1470
NmuCI	GTSAC	5 cut(s)	52, 1120, 1150, 1372, 1420
NspI	RCATGY	3 cut(s)	226, 826, 1342
PaeI	GCATGC	1 cut(s)	826
PdiI	GCCGGC	1 cut(s)	24
PdmI	GAANNNNTTC	3 cut(s)	414, 1517, 1520
PfeI	GAWTC	3 cut(s)	511, 1041, 1661
PflMI	CCANNNNNTGG	3 cut(s)	262, 506, 1414
PinAI	ACCGGT	1 cut(s)	38
PkrI	GCNGC	3 cut(s)	28, 1024, 1654
PleI	GAGTC	5 cut(s)	49, 207, 283, 339, 1713
PpsI	GAGTC	5 cut(s)	49, 207, 283, 339, 1713
PpuMI	RGGWCCY	1 cut(s)	1469
PshBI	ATTAAT	1 cut(s)	386
Psp5II	RGGWCCY	1 cut(s)	1469
Psp6I	CCWGG	4 cut(s)	62, 1036, 1324, 1471
PspGI	CCWGG	4 cut(s)	62, 1036, 1324, 1471
PspN4I	GGNNCC	5 cut(s)	329, 485, 714, 859, 1470
PspPI	GGNCC	5 cut(s)	75, 179, 282, 857, 1469
PspPPI	RGGWCCY	1 cut(s)	1469
PsuI	RGATCY	3 cut(s)	712, 1165, 1480
RseI	CAYNNNNRTG	2 cut(s)	338, 689
SacII	CCGCGG	1 cut(s)	234
SaqAI	TTAA	7 cut(s)	132, 386, 540, 581, 606, 851, 1686
SatI	GCNGC	3 cut(s)	27, 1023, 1653
Sau3AI	GATC	7 cut(s)	247, 427, 712, 1165, 1330, 1480, 1495
Sau96I	GGNCC	5 cut(s)	75, 179, 282, 857, 1469
SchI	GAGTC	5 cut(s)	49, 207, 284, 339, 1714
ScrFI	CCNGG	5 cut(s)	64, 1038, 1326, 1473, 1618
SduI	GDGCHC	4 cut(s)	16, 308, 499, 763
SexAI	ACCWGGT	1 cut(s)	1324
SfaNI	GCATC	2 cut(s)	1219, 1603
SfcI	CTRYAG	1 cut(s)	367
Sfr303I	CCGCGG	1 cut(s)	234
SgrBI	CCGCGG	1 cut(s)	234
SinI	GGWCC	3 cut(s)	75, 179, 1469
SmiMI	CAYNNNNRTG	2 cut(s)	338, 689
SmlI	CTYRAG	3 cut(s)	580, 827, 850
SmoI	CTYRAG	3 cut(s)	580, 827, 850
SphI	GCATGC	1 cut(s)	826
Sse9I	AATT	6 cut(s)	489, 637, 1073, 1359, 1443, 1516
SsiI	CCGC	5 cut(s)	73, 218, 231, 233, 703
SspI	AATATT	2 cut(s)	754, 1093
SspMI	CTAG	3 cut(s)	1103, 1169, 1256
StyD4I	CCNGG	5 cut(s)	62, 1036, 1324, 1471, 1616
StyI	CCWWGG	1 cut(s)	1408
TaaI	ACNGT	6 cut(s)	371, 746, 927, 1126, 1145, 1176
TaqI	TCGA	4 cut(s)	278, 971, 1187, 1557
TaqII	GACCGA	1 cut(s)	818
TasI	AATT	6 cut(s)	489, 637, 1073, 1359, 1443, 1516
TfiI	GAWTC	3 cut(s)	511, 1041, 1661
Tru1I	TTAA	7 cut(s)	132, 386, 540, 581, 606, 851, 1686
Tru9I	TTAA	7 cut(s)	132, 386, 540, 581, 606, 851, 1686
TscAI	CASTG	3 cut(s)	573, 1070, 1129
TseFI	GTSAC	5 cut(s)	52, 1120, 1150, 1372, 1420
TseI	GCWGC	3 cut(s)	26, 1022, 1652
Tsp45I	GTSAC	5 cut(s)	52, 1120, 1150, 1372, 1420
TspDTI	ATGAA	3 cut(s)	277, 404, 887
TspGWI	ACGGA	1 cut(s)	1473
TspRI	CASTG	3 cut(s)	573, 1070, 1129
Van91I	CCANNNNNTGG	3 cut(s)	262, 506, 1414
Vha464I	CTTAAG	2 cut(s)	580, 850
VpaK11BI	GGWCC	3 cut(s)	75, 179, 1469
VspI	ATTAAT	1 cut(s)	386
XapI	RAATTY	2 cut(s)	637, 1516
XbaI	TCTAGA	1 cut(s)	1168
XceI	RCATGY	3 cut(s)	226, 826, 1342
XcmI	CCANNNNNNNNNTGG	1 cut(s)	356
XmiI	GTMKAC	2 cut(s)	527, 846
XmnI	GAANNNNTTC	3 cut(s)	414, 1517, 1520
XspI	CTAG	3 cut(s)	1103, 1169, 1256

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.