Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0012413

Physical Location & Seq

Genomic Coordinates Reverse (-)

2472650 .. 2475036

Gene Structure 2387 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1035300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2151 bp

ATGTTTTATAATTTAGTTTTGGTACTTCTGCTGCTCTGCAACTCCCATTGCCTGGTGGATTCTTTGAACGACGAAGGCTATGCCCTTTTGTCGTTCAAGCATTCCATTGCAGAAGACCCAGAAGGGTCTCTAAGCAACTGGAACTCCTCTGATTCCAACCCCTGCACATGGAATGGGATTACATGCAAGGACCAAAGGGTTGTCTCTCTCAGCATTCCAAAGAAGAAACTTTCAGGGTCTCTTCCTTCCGCCATGGGAGCCCTCTCCGAGCTCCGCCATGTCAATTTAAGGAACAATATGTTGTACGGAAGCTTGCCCCTTGAGCTTTTCCAAGCTCTGGGGCTCCAAAGTTTGGTGCTTTATGGAAATTCCTTATCTGGGTCTGTCCCAAATGTGATTGGTAAGCTCATATACCTTCAATCCCTAGATTTATCAGAAAATTTGTTTAATGGGTCGTTGCCCTCGTCGATTATACAATGCAAGAGGCTCAGAACCATCGATCTTAGTCAGAATAATTTCACTGGCTTTCTACCAGAAGGGTTTGGAAGTAGTTTTGTTTCTCTGGAAAAACTTGATCTTTCTTTCAATAAGTTCAGTGGCTCGATTCCTAGCGATATGGGAAATCTGTCTAGCTTGCAAGGCACTGTTGATTTGTCTCATAATCTGTTTTCGGGTGCAATCCCAGCAAGCCTTGGAAATCTTCCTGAGAAGGTTTACATTGATCTTACTTACAACAATTTGAGTGGTCCGATACCGCAAAATGGTGCTCTGATGAACAGAGGACCAACTGCTTTTATTGGGAATCCTAATCTTTGTGGCCCCCCATTGAAGAACCCTTGTTCTTCTGAAATTTCCGGTGCTAGTCCACCATCCTCGATTCCGTATCTGCCAGATAACTCCCCCCCTCAGGATTCTGATGATAATGCTGGAAAGAGTGGGAAAGCAAGAGGGTTGAGTAAAAGAGCTGTCATTGCAATTATAGTGAGTGATGTAATTGGCATTTGCCTTGTTGGGCTGCTTTTCTCATATTGTTATTCGAGGTTTTGGGTTTGTAGTAAGGTTAAGGATGAAAATGGTTATGACAAGGGAGGTAAGCGAAGGAAAGAGTGCTTGTGCTTCAGGAAAGATGAATCGGAGACTTTATCGGAGAATATGGAGCACTATGATCTAGTGGCATTGGATACACTAGTGGCATTTGATCTGGATGAGCTTCTTAAGGCGTCTGCTTTTGTTCTTGGAAAAAGTGGAATTGGAATTGTTTACAAAGTTGTGCTTGAAGAAGGAATCACAGTAGCAGTGAGAAGATTGGGCGAAGGGGGCTCCCAGAGATTCAAGGAATTTCAGACCGAAGTTGAAGCAATTGGAAAGCTAAGACATCCTAATGTGGTTACTCTGAGGGCTTATTACTGGTCTGTTGATGAAAAGCTGCTCATATATGACTATATTCCTAATGGCAGCCTTGCAACCGCCATTCACGGAAAGCCAGGACCGGTATCATTTACACCAGTATCGTGGTCTGTTCGGTTGAATATCATGAAAGGAATTGCAAAAGGCTTGGTCTATCTTCATGAGTTTAGCCCCAAGAAATATGTCCATGGAGATTTGAAGCCGAATAACATACTGCTTGGACAGAACATGGAACCCCACATTTCTGATTTCGGACTTGGACGCCTTGCAAATATTGCTGGAGGGTCCCCGACACTGCAATCCAACCGAATCCCCACAGAAATATCACAAGAAAGGCAACAAACGAGTGCAGTGCCAACTGAAGCTTCTCTGGTTAGTTCGTCCAGCAATTTGGGATCTTGTTATCAGGCCCCAGAAGCTCTGAAAGTGGTGAAACCATCACAGAAGTGGGATGTGTATTCGTACGGGGTAATCTTACTCGAAATGATTACCGGAAGATTGCCAATAGTCCAAGTGGGTTCCGCAGAAATGGACCTGGTTCATTGGATTCAGCTCAACATTGAAGAGAAGAAGCCTCTTTTAGATGTTTTAGATCCAAATTTGATGCAAGATGTGGATAAGGAAGAAGAAATTATCGCGGTTCTGAAGATCGCAATGGCTTGCGTTCACAGCAGCCCTGAAAGGAGACCGATAATGAGGCACATATCCGATGCTTTGGACAGGTTGGCCACGTCTGCTGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000278 GO:0000281 GO:0000910 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007049 GO:0007275 GO:0008150 GO:0008152 GO:0008356 GO:0009505 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010069 GO:0010070 GO:0010154 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022402 GO:0022414 GO:0030312 GO:0032501 GO:0032502 GO:0036211 GO:0042995 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046777 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0051301 GO:0061458 GO:0061640 GO:0071704 GO:0071944 GO:0090406 GO:0120025 GO:0140096 GO:1901564 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

717

Amino Acids

78.1

Weight (kDa)

5.97

Isoelectric Point (pI)

41.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	22 - 62	1.1e-15	Leucine rich repeat N-terminal domain
LRR_14	PF23598	119 - 213	1.7e-06	Leucine-rich repeat region
PK_Tyr_Ser-Thr	PF07714	412 - 559	1e-20	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	412 - 704	2e-29	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	594 - 707	4.1e-07	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012413)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G01210
fragaria_vesca	FvH4_4g26710
malus_domestica	MD13G1035300.v1.1 MD16G1037100.v1.1
prunus_persica	Prupe.1G316900_v2.0.a1 Prupe.1G316900_v2.0.a1 Prupe.1G316900_v2.0.a1
pyrus_communis	pycom13g03090 pycom16g03210
rosa_chinensis	RchiOBHm_Chr4g0434401
rosa_laevigata	RLG00000006674
rosa_multiflora	Rmu_co8521181.1_g000003
rosa_roxburghii	Rroxscaffold_5G00375760
rosa_rugosa	Rorug04G0276300
rosa_samantha	Rh4AG331900 Rh4BG339700 Rh4CG354900 Rh4DG334900
rosa_wichuraiana	Rw4G028780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	9
AccB7I	CCANNNNNTGG	3 cut(s)	52, 337, 352
AccII	CGCG	1 cut(s)	2045
AciI	CCGC	6 cut(s)	249, 274, 755, 1467, 1929, 2045
AclWI	GGATC	2 cut(s)	1810, 1994
AcoI	YGGCCR	1 cut(s)	2133
AcsI	RAATTY	5 cut(s)	367, 439, 849, 1337, 2005
AcuI	CTGAAG	3 cut(s)	1102, 1788, 2072
AcyI	GRCGYC	2 cut(s)	1220, 1669
AfaI	GTAC	3 cut(s)	24, 305, 1871
AfiI	CCNNNNNNNGG	7 cut(s)	52, 168, 337, 352, 378, 761, 907
AflII	CTTAAG	1 cut(s)	1214
AgeI	ACCGGT	1 cut(s)	1489
AhlI	ACTAGT	1 cut(s)	1186
AjiI	CACGTC	1 cut(s)	2139
AjnI	CCWGG	3 cut(s)	51, 1483, 1941
AjuI	GAANNNNNNNTTGG	2 cut(s)	1756, 1788
AleI	CACNNNNGTG	1 cut(s)	1852
Alw21I	GWGCWC	3 cut(s)	273, 769, 1161
Alw26I	GTCTC	6 cut(s)	132, 208, 243, 660, 1130, 2086
AlwI	GGATC	2 cut(s)	1810, 1994
AoxI	GGCC	3 cut(s)	817, 1815, 2133
ApeKI	GCWGC	5 cut(s)	31, 1015, 1426, 1455, 2079
ApoI	RAATTY	5 cut(s)	367, 439, 849, 1337, 2005
AsiGI	ACCGGT	1 cut(s)	1489
Asp700I	GAANNNNTTC	1 cut(s)	515
AspS9I	GGNCC	8 cut(s)	190, 746, 782, 818, 1487, 1692, 1816, 1939
AsuHPI	GGTGA	1 cut(s)	1849
AvaII	GGWCC	6 cut(s)	190, 746, 782, 1487, 1692, 1939
AxyI	CCTNAGG	1 cut(s)	906
BaeI	ACNNNNGTAYC	4 cut(s)	1173, 1206, 1491, 1524
BalI	TGGCCA	1 cut(s)	2135
BanII	GRGCYC	4 cut(s)	262, 273, 345, 1322
BbsI	GAAGAC	1 cut(s)	120
Bbv12I	GWGCWC	3 cut(s)	273, 769, 1161
BbvI	GCAGC	5 cut(s)	18, 1002, 1413, 1467, 2091
BccI	CCATC	3 cut(s)	503, 877, 1852
BcgI	CGANNNNNNTGC	4 cut(s)	62, 96, 1741, 1775
BciT130I	CCWGG	3 cut(s)	53, 1485, 1943
BciVI	GTATCC	1 cut(s)	1174
BcoDI	GTCTC	6 cut(s)	132, 208, 243, 660, 1130, 2086
BcuI	ACTAGT	1 cut(s)	1186
BfaI	CTAG	6 cut(s)	425, 609, 630, 861, 1169, 1187
BfrI	CTTAAG	1 cut(s)	1214
BfuI	GTATCC	1 cut(s)	1174
BisI	GCNGC	5 cut(s)	32, 1016, 1427, 1456, 2080
BlsI	GCNGC	5 cut(s)	33, 1017, 1428, 1457, 2081
Bme1390I	CCNGG	3 cut(s)	53, 1485, 1943
Bme18I	GGWCC	6 cut(s)	190, 746, 782, 1487, 1692, 1939
BmgBI	CACGTC	1 cut(s)	2139
BmgT120I	GGNCC	8 cut(s)	190, 746, 782, 818, 1487, 1692, 1816, 1939
BmiI	GGNNCC	9 cut(s)	259, 344, 820, 1321, 1641, 1693, 1694, 1818, 1927
BmrFI	CCNGG	3 cut(s)	53, 1485, 1943
BmsI	GCATC	2 cut(s)	2001, 2107
BpiI	GAAGAC	1 cut(s)	120
BpmI	CTGGAG	1 cut(s)	1707
BpuEI	CTTGAG	1 cut(s)	341
Bsa29I	ATCGAT	1 cut(s)	498
BsaHI	GRCGYC	2 cut(s)	1220, 1669
BsaI	GGTCTC	3 cut(s)	132, 243, 2086
BsaJI	CCNNGG	3 cut(s)	252, 691, 1594
BsaWI	WCCGGW	3 cut(s)	854, 1489, 1898
BsaXI	ACNNNNNCTCC	2 cut(s)	128, 158
Bsc4I	CCNNNNNNNGG	7 cut(s)	52, 168, 337, 352, 378, 761, 907
Bse118I	RCCGGY	1 cut(s)	1489
Bse1I	ACTGG	4 cut(s)	143, 526, 1412, 1505
Bse21I	CCTNAGG	1 cut(s)	906
Bse3DI	GCAATG	4 cut(s)	46, 105, 969, 2067
BseBI	CCWGG	3 cut(s)	53, 1485, 1943
BseCI	ATCGAT	1 cut(s)	498
BseDI	CCNNGG	3 cut(s)	252, 691, 1594
BseGI	GGATG	5 cut(s)	869, 1072, 1210, 1375, 1864
BseLI	CCNNNNNNNGG	7 cut(s)	52, 168, 337, 352, 378, 761, 907
BseMI	GCAATG	4 cut(s)	46, 105, 969, 2067
BseMII	CTCAG	5 cut(s)	223, 502, 696, 920, 1385
BseNI	ACTGG	4 cut(s)	143, 526, 1412, 1505
BseRI	GAGGAG	1 cut(s)	136
BseXI	GCAGC	5 cut(s)	18, 1002, 1413, 1467, 2091
BseYI	CCCAGC	1 cut(s)	682
BsgI	GTGCAG	2 cut(s)	148, 1776
Bsh1236I	CGCG	1 cut(s)	2045
BshFI	GGCC	3 cut(s)	819, 1817, 2135
BshTI	ACCGGT	1 cut(s)	1489
BshVI	ATCGAT	1 cut(s)	498
BsiHKAI	GWGCWC	3 cut(s)	273, 769, 1161
BsiSI	CCGG	3 cut(s)	855, 1490, 1899
BsiWI	CGTACG	1 cut(s)	1869
BslFI	GGGAC	2 cut(s)	371, 1678
BslI	CCNNNNNNNGG	7 cut(s)	52, 168, 337, 352, 378, 761, 907
BsmAI	GTCTC	6 cut(s)	132, 208, 243, 660, 1130, 2086
BsmFI	GGGAC	2 cut(s)	371, 1678
BsmI	GAATGC	2 cut(s)	100, 213
BsnI	GGCC	3 cut(s)	819, 1817, 2135
Bso31I	GGTCTC	3 cut(s)	132, 243, 2086
Bsp1286I	GDGCHC	6 cut(s)	262, 273, 345, 769, 1161, 1322
Bsp143I	GATC	8 cut(s)	499, 574, 721, 1165, 1198, 1802, 1999, 2055
Bsp19I	CCATGG	2 cut(s)	252, 1594
BspACI	CCGC	6 cut(s)	249, 274, 755, 1467, 1929, 2045
BspANI	GGCC	3 cut(s)	819, 1817, 2135
BspCNI	CTCAG	5 cut(s)	222, 501, 697, 919, 1386
BspDI	ATCGAT	1 cut(s)	498
BspFNI	CGCG	1 cut(s)	2045
BspHI	TCATGA	2 cut(s)	1533, 1567
BspLI	GGNNCC	9 cut(s)	259, 344, 820, 1321, 1641, 1693, 1694, 1818, 1927
BspPI	GGATC	2 cut(s)	1810, 1994
BspTI	CTTAAG	1 cut(s)	1214
BspTNI	GGTCTC	3 cut(s)	132, 243, 2086
BsrDI	GCAATG	4 cut(s)	46, 105, 969, 2067
BsrFI	RCCGGY	1 cut(s)	1489
BsrI	ACTGG	4 cut(s)	143, 526, 1412, 1505
BssAI	RCCGGY	1 cut(s)	1489
BssECI	CCNNGG	3 cut(s)	252, 691, 1594
BssMI	GATC	8 cut(s)	499, 574, 721, 1165, 1198, 1802, 1999, 2055
BssNI	GRCGYC	2 cut(s)	1220, 1669
BssT1I	CCWWGG	3 cut(s)	252, 691, 1594
Bst2UI	CCWGG	3 cut(s)	53, 1485, 1943
Bst4CI	ACNGT	2 cut(s)	646, 1291
Bst6I	CTCTTC	2 cut(s)	246, 1965
BstACI	GRCGYC	2 cut(s)	1220, 1669
BstAFI	CTTAAG	1 cut(s)	1214
BstAPI	GCANNNNNTGC	1 cut(s)	1682
BstC8I	GCNNGC	4 cut(s)	314, 635, 688, 2068
BstDEI	CTNAG	8 cut(s)	131, 209, 488, 503, 705, 906, 1370, 1394
BstDSI	CCRYGG	2 cut(s)	252, 1594
BstF5I	GGATG	5 cut(s)	869, 1072, 1210, 1375, 1864
BstFNI	CGCG	1 cut(s)	2045
BstKTI	GATC	8 cut(s)	502, 577, 724, 1168, 1201, 1805, 2002, 2058
BstMAI	GTCTC	6 cut(s)	132, 208, 243, 660, 1130, 2086
BstMBI	GATC	8 cut(s)	499, 574, 721, 1165, 1198, 1802, 1999, 2055
BstNI	CCWGG	3 cut(s)	53, 1485, 1943
BstNSI	RCATGY	1 cut(s)	186
BstSCI	CCNGG	3 cut(s)	51, 1483, 1941
BstUI	CGCG	1 cut(s)	2045
BstV1I	GCAGC	5 cut(s)	18, 1002, 1413, 1467, 2091
BstV2I	GAAGAC	1 cut(s)	120
BstX2I	RGATCY	2 cut(s)	1802, 1999
BstXI	CCANNNNNNTGG	2 cut(s)	1512, 1798
BstYI	RGATCY	2 cut(s)	1802, 1999
Bsu15I	ATCGAT	1 cut(s)	498
Bsu36I	CCTNAGG	1 cut(s)	906
BsuI	GTATCC	1 cut(s)	1174
BsuRI	GGCC	3 cut(s)	819, 1817, 2135
BsuTUI	ATCGAT	1 cut(s)	498
BtgI	CCRYGG	2 cut(s)	252, 1594
BtrI	CACGTC	1 cut(s)	2139
BtsCI	GGATG	5 cut(s)	869, 1072, 1210, 1375, 1864
BtsI	GCAGTG	3 cut(s)	1302, 1700, 1764
BtsIMutI	CAGTG	6 cut(s)	519, 601, 642, 1302, 1700, 1764
Cac8I	GCNNGC	4 cut(s)	314, 635, 688, 2068
CciI	TCATGA	2 cut(s)	1533, 1567
Cfr10I	RCCGGY	1 cut(s)	1489
Cfr13I	GGNCC	8 cut(s)	190, 746, 782, 818, 1487, 1692, 1816, 1939
ClaI	ATCGAT	1 cut(s)	498
CseI	GACGC	2 cut(s)	1209, 1677
CsiI	ACCWGGT	1 cut(s)	1941
Csp6I	GTAC	3 cut(s)	23, 304, 1870
CspAI	ACCGGT	1 cut(s)	1489
CspCI	CAANNNNNGTGG	2 cut(s)	724, 759
CviAII	CATG	8 cut(s)	168, 183, 253, 278, 1534, 1568, 1595, 1636
CviQI	GTAC	3 cut(s)	23, 304, 1870
DdeI	CTNAG	8 cut(s)	131, 209, 488, 503, 705, 906, 1370, 1394
DpnI	GATC	8 cut(s)	501, 576, 723, 1167, 1200, 1804, 2001, 2057
DpnII	GATC	8 cut(s)	499, 574, 721, 1165, 1198, 1802, 1999, 2055
EaeI	YGGCCR	1 cut(s)	2133
Eam1104I	CTCTTC	2 cut(s)	246, 1965
EarI	CTCTTC	2 cut(s)	246, 1965
EciI	GGCGGA	2 cut(s)	238, 263
Ecl136II	GAGCTC	1 cut(s)	271
Eco130I	CCWWGG	3 cut(s)	252, 691, 1594
Eco24I	GRGCYC	4 cut(s)	262, 273, 345, 1322
Eco31I	GGTCTC	3 cut(s)	132, 243, 2086
Eco47I	GGWCC	6 cut(s)	190, 746, 782, 1487, 1692, 1939
Eco53kI	GAGCTC	1 cut(s)	271
Eco57I	CTGAAG	3 cut(s)	1102, 1788, 2072
Eco81I	CCTNAGG	1 cut(s)	906
EcoICRI	GAGCTC	1 cut(s)	271
EcoO109I	RGGNCCY	2 cut(s)	1692, 1816
EcoRII	CCWGG	3 cut(s)	51, 1483, 1941
EcoT14I	CCWWGG	3 cut(s)	252, 691, 1594
EcoT38I	GRGCYC	4 cut(s)	262, 273, 345, 1322
ErhI	CCWWGG	3 cut(s)	252, 691, 1594
FaeI	CATG	8 cut(s)	171, 186, 256, 281, 1537, 1571, 1598, 1639
FalI	AAGNNNNNCTT	2 cut(s)	1257, 1289
FaqI	GGGAC	2 cut(s)	371, 1678
FatI	CATG	8 cut(s)	167, 182, 252, 277, 1533, 1567, 1594, 1635
Fnu4HI	GCNGC	5 cut(s)	32, 1016, 1427, 1456, 2080
FokI	GGATG	5 cut(s)	856, 1079, 1217, 1362, 1871
FriOI	GRGCYC	4 cut(s)	262, 273, 345, 1322
Fsp4HI	GCNGC	5 cut(s)	32, 1016, 1427, 1456, 2080
FspBI	CTAG	6 cut(s)	425, 609, 630, 861, 1169, 1187
GluI	GCNGC	5 cut(s)	32, 1016, 1427, 1456, 2080
GsaI	CCCAGC	1 cut(s)	686
GsuI	CTGGAG	1 cut(s)	1707
HaeIII	GGCC	3 cut(s)	819, 1817, 2135
HapII	CCGG	3 cut(s)	855, 1490, 1899
HgaI	GACGC	2 cut(s)	1209, 1677
Hin1I	GRCGYC	2 cut(s)	1220, 1669
Hin1II	CATG	8 cut(s)	171, 186, 256, 281, 1537, 1571, 1598, 1639
HindIII	AAGCTT	2 cut(s)	310, 1770
HpaII	CCGG	3 cut(s)	855, 1490, 1899
HphI	GGTGA	1 cut(s)	1849
Hpy166II	GTNNAC	4 cut(s)	715, 866, 1261, 2074
Hpy188III	TCNNGA	7 cut(s)	563, 704, 908, 1120, 1202, 1534, 1568
Hpy8I	GTNNAC	4 cut(s)	715, 866, 1261, 2074
Hpy99I	CGWCG	2 cut(s)	74, 469
HpyAV	CCTTC	9 cut(s)	68, 116, 255, 425, 530, 703, 1092, 1274, 1307
HpyCH4III	ACNGT	2 cut(s)	646, 1291
HpyCH4IV	ACGT	1 cut(s)	2138
HpyF3I	CTNAG	8 cut(s)	131, 209, 488, 503, 705, 906, 1370, 1394
HpySE526I	ACGT	1 cut(s)	2138
Hsp92I	GRCGYC	2 cut(s)	1220, 1669
Hsp92II	CATG	8 cut(s)	171, 186, 256, 281, 1537, 1571, 1598, 1639
KflI	GGGWCCC	1 cut(s)	1692
Kzo9I	GATC	8 cut(s)	499, 574, 721, 1165, 1198, 1802, 1999, 2055
LmnI	GCTCC	5 cut(s)	257, 276, 348, 1156, 1325
Lsp1109I	GCAGC	5 cut(s)	18, 1002, 1413, 1467, 2091
LweI	GCATC	2 cut(s)	2001, 2107
MabI	ACCWGGT	1 cut(s)	1941
MaeI	CTAG	6 cut(s)	425, 609, 630, 861, 1169, 1187
MaeII	ACGT	1 cut(s)	2138
MaeIII	GTNAC	1 cut(s)	1387
MalI	GATC	8 cut(s)	501, 576, 723, 1167, 1200, 1804, 2001, 2057
MboI	GATC	8 cut(s)	499, 574, 721, 1165, 1198, 1802, 1999, 2055
MfeI	CAATTG	1 cut(s)	1359
MflI	RGATCY	2 cut(s)	1802, 1999
MhlI	GDGCHC	6 cut(s)	262, 273, 345, 769, 1161, 1322
MlsI	TGGCCA	1 cut(s)	2135
MluNI	TGGCCA	1 cut(s)	2135
MmeI	TCCRAC	2 cut(s)	180, 1734
Mox20I	TGGCCA	1 cut(s)	2135
MroXI	GAANNNNTTC	1 cut(s)	515
MscI	TGGCCA	1 cut(s)	2135
MseI	TTAA	4 cut(s)	287, 447, 1062, 1215
MslI	CAYNNNNRTG	2 cut(s)	1380, 1852
Msp20I	TGGCCA	1 cut(s)	2135
MspCI	CTTAAG	1 cut(s)	1214
MspI	CCGG	3 cut(s)	855, 1490, 1899
MspR9I	CCNGG	3 cut(s)	53, 1485, 1943
MunI	CAATTG	1 cut(s)	1359
Mva1269I	GAATGC	2 cut(s)	100, 213
MvaI	CCWGG	3 cut(s)	53, 1485, 1943
MvnI	CGCG	1 cut(s)	2045
NcoI	CCATGG	2 cut(s)	252, 1594
NdeII	GATC	8 cut(s)	499, 574, 721, 1165, 1198, 1802, 1999, 2055
NlaIII	CATG	8 cut(s)	171, 186, 256, 281, 1537, 1571, 1598, 1639
NlaIV	GGNNCC	9 cut(s)	259, 344, 820, 1321, 1641, 1693, 1694, 1818, 1927
NspI	RCATGY	1 cut(s)	186
OliI	CACNNNNGTG	1 cut(s)	1852
PagI	TCATGA	2 cut(s)	1533, 1567
PcsI	WCGNNNNNNNCGW	1 cut(s)	461
PctI	GAATGC	2 cut(s)	100, 213
PdmI	GAANNNNTTC	1 cut(s)	515
Pfl23II	CGTACG	1 cut(s)	1869
PflMI	CCANNNNNTGG	3 cut(s)	52, 337, 352
PinAI	ACCGGT	1 cut(s)	1489
PkrI	GCNGC	5 cut(s)	33, 1017, 1428, 1457, 2081
PpuMI	RGGWCCY	1 cut(s)	1692
PsiI	TTATAA	1 cut(s)	9
Psp124BI	GAGCTC	1 cut(s)	273
Psp5II	RGGWCCY	1 cut(s)	1692
Psp6I	CCWGG	3 cut(s)	51, 1483, 1941
PspFI	CCCAGC	1 cut(s)	682
PspGI	CCWGG	3 cut(s)	51, 1483, 1941
PspLI	CGTACG	1 cut(s)	1869
PspN4I	GGNNCC	9 cut(s)	259, 344, 820, 1321, 1641, 1693, 1694, 1818, 1927
PspPI	GGNCC	8 cut(s)	190, 746, 782, 818, 1487, 1692, 1816, 1939
PspPPI	RGGWCCY	1 cut(s)	1692
PsuI	RGATCY	2 cut(s)	1802, 1999
RsaI	GTAC	3 cut(s)	24, 305, 1871
RsaNI	GTAC	3 cut(s)	23, 304, 1870
RseI	CAYNNNNRTG	2 cut(s)	1380, 1852
SacI	GAGCTC	1 cut(s)	273
SaqAI	TTAA	4 cut(s)	287, 447, 1062, 1215
SatI	GCNGC	5 cut(s)	32, 1016, 1427, 1456, 2080
Sau3AI	GATC	8 cut(s)	499, 574, 721, 1165, 1198, 1802, 1999, 2055
Sau96I	GGNCC	8 cut(s)	190, 746, 782, 818, 1487, 1692, 1816, 1939
ScrFI	CCNGG	3 cut(s)	53, 1485, 1943
SduI	GDGCHC	6 cut(s)	262, 273, 345, 769, 1161, 1322
SexAI	ACCWGGT	1 cut(s)	1941
SfaNI	GCATC	2 cut(s)	2001, 2107
SinI	GGWCC	6 cut(s)	190, 746, 782, 1487, 1692, 1939
SmiMI	CAYNNNNRTG	2 cut(s)	1380, 1852
SmlI	CTYRAG	2 cut(s)	320, 1214
SmoI	CTYRAG	2 cut(s)	320, 1214
SpeI	ACTAGT	1 cut(s)	1186
SsiI	CCGC	6 cut(s)	249, 274, 755, 1467, 1929, 2045
SspI	AATATT	1 cut(s)	1681
SspMI	CTAG	6 cut(s)	425, 609, 630, 861, 1169, 1187
SstI	GAGCTC	1 cut(s)	273
StyD4I	CCNGG	3 cut(s)	51, 1483, 1941
StyI	CCWWGG	3 cut(s)	252, 691, 1594
TaaI	ACNGT	2 cut(s)	646, 1291
TaiI	ACGT	1 cut(s)	2141
TaqI	TCGA	6 cut(s)	467, 498, 602, 875, 1037, 1887
TaqII	GACCGA	2 cut(s)	1361, 2110
Tru1I	TTAA	4 cut(s)	287, 447, 1062, 1215
Tru9I	TTAA	4 cut(s)	287, 447, 1062, 1215
TscAI	CASTG	6 cut(s)	526, 601, 649, 1302, 1707, 1764
TseI	GCWGC	5 cut(s)	31, 1015, 1426, 1455, 2079
TspDTI	ATGAA	7 cut(s)	788, 1083, 1143, 1434, 1550, 1556, 1937
TspGWI	ACGGA	3 cut(s)	321, 870, 1491
TspRI	CASTG	6 cut(s)	526, 601, 649, 1302, 1707, 1764
Van91I	CCANNNNNTGG	3 cut(s)	52, 337, 352
Vha464I	CTTAAG	1 cut(s)	1214
VpaK11BI	GGWCC	6 cut(s)	190, 746, 782, 1487, 1692, 1939
XapI	RAATTY	5 cut(s)	367, 439, 849, 1337, 2005
XceI	RCATGY	1 cut(s)	186
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1851
XmnI	GAANNNNTTC	1 cut(s)	515
XspI	CTAG	6 cut(s)	425, 609, 630, 861, 1169, 1187

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.