MD13G1086300.v1.1

IAA-amino acid hydrolase ILR1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0015613
Physical Location & Seq
Forward (+)
6072850 .. 6075128
2279 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1086300.v1.1.491

Sequence Viewer

Length: 1347 bp
ATGGACTTTCTTCTTCTGCAACTTTTGTTCCTAATAATCTCCATCGCCATGCCCTTGTGCTTTTCTCTGAAACTCCAAAGTAACCCAACCGATCATCTCAACTCGTTCTCCACATATCAGACTTATTCAGTGAAAGACCAGATAGTTAGGCTGGCCAATGAACCCAGCACAGTGAATTGGATGAAAAGTGTGAGGAGGGAAATGCATGAGAACCCAGAACTTTCATATGAAGAATTCGAAACAAGTGCACTCATTCGCCGGGAGCTCGAAAAACTCGGTGTTGCTTATAAATGGCCTGTGGCTAAAACTGGCGTTGTAGCCACGATTGGCTCTGGCTCTCCTCCCTTTGTTGCTCTGAGAGCTGATATGGATGCTTTGCCTATTGAGGAAATGGTGGACTGGGAGAACAAGAGCAAAGTGGAGGGAAAAATGCATGCTTGCGGCCACGACGGCCACGTCGCCATGCTTCTTGGCGCTGCAAAAATACTAAAACAACTGCAAGACACATTACAGGGAACTGTTGTACTTATATTTCAACCGGCTGAGGAACGCGGTGTAGGCGCAAAGGACATGATTGAAGAAGGGGTGCTTGATAATGTGGAGGCAGTTTTTGGGATGCACATAGTGCTTGATTATCCGACTGGCGTTGTTGGATTGAGGCCTGGAGAATTTCTAGCCGGTTGTGGGAGCTTCAAGGCGAAAATTCACGGGAAAGGGGGTCATGCAGCAATTCCTCAGCAATCCATTGATCCGATTTTGGCAGCATCAACATCTGTAATCAGTTTGCAGAGCATCGTCTCGAGGGAAACAGACCCTCTAGATTCCCAGGTGGTTTCTGTTTCTGTGATTAAAGCGGGGTCTTCCTTTAATGTCATCCCGGAATCAGCTACAATTTCTGGTACTTTTAGAGCTTTTAGCAAGAAGAACTTCAAGGGACTCATGAATAGAATCGAAGAGGTTGTAAAAGGGCAGGCAGCCGTGCACCGGTGCTCCGCTGAGGTCGAGTTTTTAGGGAAGGAACATCCTACCATCCCTCCTACTGTAAACGACGAGAGAATTTATGAACAAGTAAGGGGAATCTCGAGCGAAATAGTAGGAGAAGAAAACATACAACTAGCTCATACCTCCATGGGGAGTGAGGATTTTGCTTTTTACTTAGACAAGGTGCCTGGATTCATGCTGTTTTTAGGCACAAGAAATGAGAAGATAGGAGCCATACATGCACCACATAGTCCTAACTACATCATCGATGAGGATGTGTTTCCCATTGGGGCTGCCATACATGCAGCTTTTGCCCATTCTTTCCTATCAGATTCAACTGTAAAGTTGCACCTTCATAATTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
M20_dimer Peptidase_M20

Protein Analysis

449

Amino Acids

49.05

Weight (kDa)

5.58

Isoelectric Point (pI)

44.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M20 PF01546 118 - 432 1.3e-46 Peptidase family M20/M25/M40
M20_dimer PF07687 227 - 325 4.3e-12 Peptidase dimerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015613)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g35100
malus_domestica MD13G1086300.v1.1 MD16G1085700.v1.1
prunus_persica Prupe.1G260400_v2.0.a1
pyrus_communis pycom13g07450 pycom16g07400
rosa_chinensis RchiOBHm_Chr4g0444701
rosa_laevigata RLG00000005812
rosa_multiflora Rmu_sc0012443.1_g000011
rosa_roxburghii Rroxscaffold_5G00385200
rosa_rugosa Rorug04G0354600
rosa_samantha Rh4AG414700 Rh4BG426400 Rh4CG441300 Rh4DG422300
rosa_wichuraiana Rw4G035630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 288
AasI GACNNNNNNGTC 1 cut(s) 455
AccB1I GGYRCC 1 cut(s) 1165
AccII CGCG 1 cut(s) 552
AciI CCGC 4 cut(s) 441, 552, 854, 993
AclWI GGATC 1 cut(s) 743
AcoI YGGCCR 3 cut(s) 153, 442, 451
AcsI RAATTY 4 cut(s) 233, 668, 702, 1056
AdeI CACNNNGTG 1 cut(s) 625
AfaI GTAC 2 cut(s) 525, 901
AfiI CCNNNNNNNGG 3 cut(s) 684, 984, 1131
AgeI ACCGGT 1 cut(s) 984
AgsI TTSAA 5 cut(s) 536, 578, 694, 931, 1317
AjiI CACGTC 1 cut(s) 457
AjnI CCWGG 3 cut(s) 661, 825, 1168
AluBI AGCT 7 cut(s) 265, 362, 690, 887, 911, 1118, 1289
AluI AGCT 7 cut(s) 265, 362, 690, 887, 911, 1118, 1289
Alw21I GWGCWC 4 cut(s) 250, 267, 984, 992
Alw26I GTCTC 1 cut(s) 802
Alw44I GTGCAC 2 cut(s) 246, 980
AlwI GGATC 1 cut(s) 743
Ama87I CYCGRG 2 cut(s) 799, 1081
AoxI GGCC 5 cut(s) 153, 293, 442, 451, 659
ApaLI GTGCAC 2 cut(s) 246, 980
ApeKI GCWGC 6 cut(s) 476, 725, 761, 974, 1274, 1286
ApoI RAATTY 4 cut(s) 233, 668, 702, 1056
AsiGI ACCGGT 1 cut(s) 984
Asp700I GAANNNNTTC 1 cut(s) 926
AspLEI GCGC 2 cut(s) 476, 563
AsuC2I CCSGG 2 cut(s) 260, 878
AsuII TTCGAA 1 cut(s) 237
AvaI CYCGRG 2 cut(s) 799, 1081
BaeGI GKGCMC 2 cut(s) 250, 984
BalI TGGCCA 1 cut(s) 155
BanI GGYRCC 1 cut(s) 1165
BanII GRGCYC 1 cut(s) 267
BarI GAAGNNNNNNTAC 2 cut(s) 1092, 1124
BbsI GAAGAC 1 cut(s) 852
Bbv12I GWGCWC 4 cut(s) 250, 267, 984, 992
BbvCI CCTCAGC 3 cut(s) 543, 735, 996
BbvI GCAGC 6 cut(s) 463, 737, 773, 986, 1261, 1298
BccI CCATC 2 cut(s) 50, 1037
BceAI ACGGC 2 cut(s) 466, 962
BcgI CGANNNNNNTGC 2 cut(s) 227, 261
BciT130I CCWGG 3 cut(s) 663, 827, 1170
BcnI CCSGG 2 cut(s) 260, 878
BcoDI GTCTC 1 cut(s) 802
BfaI CTAG 3 cut(s) 674, 818, 1115
BfoI RGCGCY 1 cut(s) 477
BglI GCCNNNNNGGC 1 cut(s) 450
BisI GCNGC 7 cut(s) 442, 477, 726, 762, 975, 1275, 1287
BlsI GCNGC 7 cut(s) 443, 478, 727, 763, 976, 1276, 1288
Bme1390I CCNGG 5 cut(s) 260, 663, 827, 878, 1170
BmeT110I CYCGRG 2 cut(s) 799, 1081
BmgBI CACGTC 1 cut(s) 457
BmiI GGNNCC 2 cut(s) 1167, 1213
BmrFI CCNGG 5 cut(s) 260, 663, 827, 878, 1170
BmrI ACTGGG 1 cut(s) 409
BmsI GCATC 4 cut(s) 361, 606, 773, 801
BmuI ACTGGG 1 cut(s) 409
BpiI GAAGAC 1 cut(s) 852
BpmI CTGGAG 1 cut(s) 684
Bpu10I CCTNAGC 3 cut(s) 543, 735, 996
Bpu14I TTCGAA 1 cut(s) 237
BpuMI CCSGG 2 cut(s) 260, 878
Bsa29I ATCGAT 1 cut(s) 1248
BsaJI CCNNGG 2 cut(s) 825, 1128
BsaWI WCCGGW 1 cut(s) 984
BsaXI ACNNNNNCTCC 6 cut(s) 92, 122, 974, 1004, 1018, 1048
Bsc4I CCNNNNNNNGG 3 cut(s) 684, 984, 1131
Bse118I RCCGGY 3 cut(s) 538, 677, 984
Bse1I ACTGG 3 cut(s) 313, 404, 646
BseBI CCWGG 3 cut(s) 663, 827, 1170
BseCI ATCGAT 1 cut(s) 1248
BseDI CCNNGG 2 cut(s) 825, 1128
BseGI GGATG 7 cut(s) 186, 376, 621, 873, 1021, 1029, 1261
BseLI CCNNNNNNNGG 3 cut(s) 684, 984, 1131
BseMII CTCAG 4 cut(s) 347, 534, 749, 987
BseNI ACTGG 3 cut(s) 313, 404, 646
BseRI GAGGAG 2 cut(s) 208, 330
BseSI GKGCMC 2 cut(s) 250, 984
BseXI GCAGC 6 cut(s) 463, 737, 773, 986, 1261, 1298
BseYI CCCAGC 1 cut(s) 164
Bsh1236I CGCG 1 cut(s) 552
BshFI GGCC 5 cut(s) 155, 295, 444, 453, 661
BshNI GGYRCC 1 cut(s) 1165
BshTI ACCGGT 1 cut(s) 984
BshVI ATCGAT 1 cut(s) 1248
BsiHKAI GWGCWC 4 cut(s) 250, 267, 984, 992
BsiHKCI CYCGRG 2 cut(s) 799, 1081
BsiSI CCGG 5 cut(s) 259, 539, 678, 878, 985
BslFI GGGAC 1 cut(s) 948
BslI CCNNNNNNNGG 3 cut(s) 684, 984, 1131
BsmAI GTCTC 1 cut(s) 802
BsmBI CGTCTC 1 cut(s) 802
BsmFI GGGAC 1 cut(s) 948
BsnI GGCC 5 cut(s) 155, 295, 444, 453, 661
BsoBI CYCGRG 2 cut(s) 799, 1081
Bsp119I TTCGAA 1 cut(s) 237
Bsp1286I GDGCHC 4 cut(s) 250, 267, 984, 992
Bsp143I GATC 2 cut(s) 91, 748
Bsp19I CCATGG 1 cut(s) 1128
BspACI CCGC 4 cut(s) 441, 552, 854, 993
BspANI GGCC 5 cut(s) 155, 295, 444, 453, 661
BspCNI CTCAG 4 cut(s) 348, 535, 748, 988
BspDI ATCGAT 1 cut(s) 1248
BspFNI CGCG 1 cut(s) 552
BspHI TCATGA 1 cut(s) 939
BspLI GGNNCC 2 cut(s) 1167, 1213
BspPI GGATC 1 cut(s) 743
BspT104I TTCGAA 1 cut(s) 237
BspT107I GGYRCC 1 cut(s) 1165
BsrFI RCCGGY 3 cut(s) 538, 677, 984
BsrI ACTGG 3 cut(s) 313, 404, 646
BssAI RCCGGY 3 cut(s) 538, 677, 984
BssECI CCNNGG 2 cut(s) 825, 1128
BssMI GATC 2 cut(s) 91, 748
BssT1I CCWWGG 1 cut(s) 1128
Bst2UI CCWGG 3 cut(s) 663, 827, 1170
Bst4CI ACNGT 4 cut(s) 172, 520, 1042, 1321
Bst6I CTCTTC 1 cut(s) 948
BstAPI GCANNNNNTGC 2 cut(s) 625, 1292
BstBI TTCGAA 1 cut(s) 237
BstC8I GCNNGC 4 cut(s) 153, 435, 439, 972
BstDEI CTNAG 5 cut(s) 356, 543, 735, 996, 1156
BstDSI CCRYGG 1 cut(s) 1128
BstF5I GGATG 7 cut(s) 186, 376, 621, 873, 1021, 1029, 1261
BstFNI CGCG 1 cut(s) 552
BstH2I RGCGCY 1 cut(s) 477
BstHHI GCGC 2 cut(s) 476, 563
BstKTI GATC 2 cut(s) 94, 751
BstMAI GTCTC 1 cut(s) 802
BstMBI GATC 2 cut(s) 91, 748
BstMWI GCNNNNNNNGC 7 cut(s) 359, 450, 558, 625, 1220, 1283, 1292
BstNI CCWGG 3 cut(s) 663, 827, 1170
BstNSI RCATGY 3 cut(s) 437, 1223, 1286
BstSCI CCNGG 5 cut(s) 258, 661, 825, 876, 1168
BstSLI GKGCMC 2 cut(s) 250, 984
BstUI CGCG 1 cut(s) 552
BstV1I GCAGC 6 cut(s) 463, 737, 773, 986, 1261, 1298
BstV2I GAAGAC 1 cut(s) 852
Bsu15I ATCGAT 1 cut(s) 1248
BsuRI GGCC 5 cut(s) 155, 295, 444, 453, 661
BsuTUI ATCGAT 1 cut(s) 1248
BtgI CCRYGG 1 cut(s) 1128
BtgZI GCGATG 1 cut(s) 28
BtrI CACGTC 1 cut(s) 457
BtsCI GGATG 7 cut(s) 186, 376, 621, 873, 1021, 1029, 1261
BtsIMutI CAGTG 2 cut(s) 135, 177
Cac8I GCNNGC 4 cut(s) 153, 435, 439, 972
CciI TCATGA 1 cut(s) 939
CfoI GCGC 2 cut(s) 476, 563
Cfr10I RCCGGY 3 cut(s) 538, 677, 984
ClaI ATCGAT 1 cut(s) 1248
Csp6I GTAC 2 cut(s) 524, 900
CspAI ACCGGT 1 cut(s) 984
CviQI GTAC 2 cut(s) 524, 900
DdeI CTNAG 5 cut(s) 356, 543, 735, 996, 1156
DpnI GATC 2 cut(s) 93, 750
DpnII GATC 2 cut(s) 91, 748
DraIII CACNNNGTG 1 cut(s) 625
DrdI GACNNNNNNGTC 1 cut(s) 455
DseDI GACNNNNNNGTC 1 cut(s) 455
EaeI YGGCCR 3 cut(s) 153, 442, 451
Eam1104I CTCTTC 1 cut(s) 948
EarI CTCTTC 1 cut(s) 948
Ecl136II GAGCTC 1 cut(s) 265
Eco130I CCWWGG 1 cut(s) 1128
Eco147I AGGCCT 1 cut(s) 661
Eco24I GRGCYC 1 cut(s) 267
Eco53kI GAGCTC 1 cut(s) 265
Eco88I CYCGRG 2 cut(s) 799, 1081
EcoICRI GAGCTC 1 cut(s) 265
EcoRI GAATTC 1 cut(s) 233
EcoRII CCWGG 3 cut(s) 661, 825, 1168
EcoT14I CCWWGG 1 cut(s) 1128
EcoT22I ATGCAT 2 cut(s) 207, 435
EcoT38I GRGCYC 1 cut(s) 267
ErhI CCWWGG 1 cut(s) 1128
Esp3I CGTCTC 1 cut(s) 802
FalI AAGNNNNNCTT 4 cut(s) 573, 605, 911, 943
FaqI GGGAC 1 cut(s) 948
FauI CCCGC 1 cut(s) 847
FauNDI CATATG 1 cut(s) 226
Fnu4HI GCNGC 7 cut(s) 442, 477, 726, 762, 975, 1275, 1287
FokI GGATG 7 cut(s) 193, 383, 628, 860, 1008, 1016, 1268
FriOI GRGCYC 1 cut(s) 267
Fsp4HI GCNGC 7 cut(s) 442, 477, 726, 762, 975, 1275, 1287
FspBI CTAG 3 cut(s) 674, 818, 1115
GlaI GCGC 2 cut(s) 475, 562
GluI GCNGC 7 cut(s) 442, 477, 726, 762, 975, 1275, 1287
GsaI CCCAGC 1 cut(s) 168
GsuI CTGGAG 1 cut(s) 684
HaeII RGCGCY 1 cut(s) 477
HaeIII GGCC 5 cut(s) 155, 295, 444, 453, 661
HapII CCGG 5 cut(s) 259, 539, 678, 878, 985
HhaI GCGC 2 cut(s) 476, 563
Hin6I GCGC 2 cut(s) 474, 561
HinP1I GCGC 2 cut(s) 474, 561
HinfI GANTC 7 cut(s) 821, 881, 936, 948, 1077, 1173, 1313
HpaII CCGG 5 cut(s) 259, 539, 678, 878, 985
Hpy166II GTNNAC 4 cut(s) 248, 397, 982, 1045
Hpy188I TCNGA 7 cut(s) 69, 120, 357, 639, 753, 1312, 1346
Hpy188III TCNNGA 4 cut(s) 799, 818, 940, 1081
Hpy8I GTNNAC 4 cut(s) 248, 397, 982, 1045
Hpy99I CGWCG 3 cut(s) 452, 461, 1052
HpyAV CCTTC 3 cut(s) 575, 1009, 1343
HpyCH4III ACNGT 4 cut(s) 172, 520, 1042, 1321
HpyCH4IV ACGT 1 cut(s) 456
HpyF10VI GCNNNNNNNGC 7 cut(s) 359, 450, 558, 625, 1220, 1283, 1292
HpyF3I CTNAG 5 cut(s) 356, 543, 735, 996, 1156
HpySE526I ACGT 1 cut(s) 456
HspAI GCGC 2 cut(s) 474, 561
Kzo9I GATC 2 cut(s) 91, 748
LmnI GCTCC 4 cut(s) 262, 687, 995, 1211
Lsp1109I GCAGC 6 cut(s) 463, 737, 773, 986, 1261, 1298
LweI GCATC 4 cut(s) 361, 606, 773, 801
MaeI CTAG 3 cut(s) 674, 818, 1115
MaeII ACGT 1 cut(s) 456
MaeIII GTNAC 1 cut(s) 80
MalI GATC 2 cut(s) 93, 750
MboI GATC 2 cut(s) 91, 748
MboII GAAGA 8 cut(s) 5, 242, 590, 852, 934, 965, 1112, 1216
MhlI GDGCHC 4 cut(s) 250, 267, 984, 992
MlsI TGGCCA 1 cut(s) 155
MluCI AATT 8 cut(s) 175, 233, 668, 702, 729, 891, 1056, 1339
MluNI TGGCCA 1 cut(s) 155
MlyI GAGTC 1 cut(s) 930
MmeI TCCRAC 2 cut(s) 631, 662
Mox20I TGGCCA 1 cut(s) 155
Mph1103I ATGCAT 2 cut(s) 207, 435
MroXI GAANNNNTTC 1 cut(s) 926
MscI TGGCCA 1 cut(s) 155
MseI TTAA 2 cut(s) 849, 867
MslI CAYNNNNRTG 1 cut(s) 47
Msp20I TGGCCA 1 cut(s) 155
MspA1I CMGCKG 1 cut(s) 995
MspI CCGG 5 cut(s) 259, 539, 678, 878, 985
MspR9I CCNGG 5 cut(s) 260, 663, 827, 878, 1170
MvaI CCWGG 3 cut(s) 663, 827, 1170
MvnI CGCG 1 cut(s) 552
MwoI GCNNNNNNNGC 7 cut(s) 359, 450, 558, 625, 1220, 1283, 1292
NciI CCSGG 2 cut(s) 260, 878
NcoI CCATGG 1 cut(s) 1128
NdeI CATATG 1 cut(s) 226
NdeII GATC 2 cut(s) 91, 748
NlaIV GGNNCC 2 cut(s) 1167, 1213
NsiI ATGCAT 2 cut(s) 207, 435
NspI RCATGY 3 cut(s) 437, 1223, 1286
NspV TTCGAA 1 cut(s) 237
PaeI GCATGC 1 cut(s) 437
PaeR7I CTCGAG 2 cut(s) 799, 1081
PagI TCATGA 1 cut(s) 939
PceI AGGCCT 1 cut(s) 661
PcsI WCGNNNNNNNCGW 1 cut(s) 453
PdmI GAANNNNTTC 1 cut(s) 926
PfeI GAWTC 6 cut(s) 821, 881, 948, 1077, 1173, 1313
PfoI TCCNGGA 1 cut(s) 876
PinAI ACCGGT 1 cut(s) 984
PkrI GCNGC 7 cut(s) 443, 478, 727, 763, 976, 1276, 1288
PleI GAGTC 1 cut(s) 930
PpsI GAGTC 1 cut(s) 930
PsiI TTATAA 1 cut(s) 288
Psp124BI GAGCTC 1 cut(s) 267
Psp6I CCWGG 3 cut(s) 661, 825, 1168
PspFI CCCAGC 1 cut(s) 164
PspGI CCWGG 3 cut(s) 661, 825, 1168
PspN4I GGNNCC 2 cut(s) 1167, 1213
RsaI GTAC 2 cut(s) 525, 901
RsaNI GTAC 2 cut(s) 524, 900
RseI CAYNNNNRTG 1 cut(s) 47
SacI GAGCTC 1 cut(s) 267
SaqAI TTAA 2 cut(s) 849, 867
SatI GCNGC 7 cut(s) 442, 477, 726, 762, 975, 1275, 1287
Sau3AI GATC 2 cut(s) 91, 748
SchI GAGTC 1 cut(s) 930
ScrFI CCNGG 5 cut(s) 260, 663, 827, 878, 1170
SduI GDGCHC 4 cut(s) 250, 267, 984, 992
SfaNI GCATC 4 cut(s) 361, 606, 773, 801
SfiI GGCCNNNNNGGCC 1 cut(s) 450
Sfr274I CTCGAG 2 cut(s) 799, 1081
SfuI TTCGAA 1 cut(s) 237
SgrAI CRCCGGYG 1 cut(s) 984
SlaI CTCGAG 2 cut(s) 799, 1081
SmiMI CAYNNNNRTG 1 cut(s) 47
SmlI CTYRAG 2 cut(s) 799, 1081
SmoI CTYRAG 2 cut(s) 799, 1081
SphI GCATGC 1 cut(s) 437
Sse9I AATT 8 cut(s) 175, 233, 668, 702, 729, 891, 1056, 1339
SseBI AGGCCT 1 cut(s) 661
SsiI CCGC 4 cut(s) 441, 552, 854, 993
SspMI CTAG 3 cut(s) 674, 818, 1115
SstI GAGCTC 1 cut(s) 267
StuI AGGCCT 1 cut(s) 661
StyD4I CCNGG 5 cut(s) 258, 661, 825, 876, 1168
StyI CCWWGG 1 cut(s) 1128
TaaI ACNGT 4 cut(s) 172, 520, 1042, 1321
TaiI ACGT 1 cut(s) 459
TaqI TCGA 7 cut(s) 237, 267, 800, 951, 1002, 1082, 1248
TasI AATT 8 cut(s) 175, 233, 668, 702, 729, 891, 1056, 1339
TatI WGTACW 1 cut(s) 523
TauI GCSGC 1 cut(s) 444
TfiI GAWTC 6 cut(s) 821, 881, 948, 1077, 1173, 1313
Tru1I TTAA 2 cut(s) 849, 867
Tru9I TTAA 2 cut(s) 849, 867
TscAI CASTG 2 cut(s) 135, 177
TseI GCWGC 6 cut(s) 476, 725, 761, 974, 1274, 1286
TspDTI ATGAA 8 cut(s) 174, 197, 213, 243, 956, 1077, 1165, 1325
TspRI CASTG 2 cut(s) 135, 177
VneI GTGCAC 2 cut(s) 246, 980
XapI RAATTY 4 cut(s) 233, 668, 702, 1056
XbaI TCTAGA 1 cut(s) 817
XceI RCATGY 3 cut(s) 437, 1223, 1286
XhoI CTCGAG 2 cut(s) 799, 1081
XmnI GAANNNNTTC 1 cut(s) 926
XspI CTAG 3 cut(s) 674, 818, 1115
Zsp2I ATGCAT 2 cut(s) 207, 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.