Prupe.1G260400_v2.0.a1

IAA-amino acid hydrolase ILR1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0015613
Physical Location & Seq
Reverse (-)
26939949 .. 26942739
2791 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G260400.1

Sequence Viewer

Length: 1299 bp
ATGGACCTTCTGCAACTTTTGTTCCTAATCTCCATTGCCACAACCACAACCTTGTGCTTGTCTACCACATATCAGGATTATTCATTGAAAAAGCAGATAGTTGGGCTGGCCAATGAGTTTCACACAGTAAATTGGATGAAGAATGTGAGGAGAGAAATCCATGAAAACCCAGAACTTGCATATGAAGAATTCAAAACAAGCGCACTCATTAGGCGCGAGCTTGACAAGATTGGTGTTGCATATACATGGCCTGTGGCGCATACTGGTGTGGTGGCCACTATTGGCTCTGGCTCTCCTCCCTTTGTTGCTCTGAGAGCAGATATGGATGCTTTGCCCATTCAGGAATTGGTGGAATGGGAGCACAAGAGCAAAGTGGACGGAAAAATGCATGCTTGTGGCCACGACGCACATGTTGCCATGCTTCTTGGTGCTGCTAAAGTACTTCAACAATTGAAAGACACATTACAGGGAACTGTTGTTCTTATATTTCAACCAGCTGAGGAACGCGGCATAGGTGCGAAGGACATGATCAAAGAAGGGGTGCTTGATAATGTGGAGGCCATCTTTGGGTTGCACGTAGTGCATAGATATCCAAGTGGTGTTGTTGCATCAAGGCCTGGAGAATTTCTAGCTGGTTGTGGGAGCTTCAAAGCAAAGATTCATGGAAAAGGGGGTCATGCAGCAATTCCTCAACAATCCATTGATCCAATTGTGGCAGCATCAGCATCAGTGATTAGCTTGCAAAACATCGTCTCGAGAGAGGCAGACCCTTTAGATTCCCAGGTGGTTTCTGTAGCTATGATTCAAGCAGGGACTTCATTTAACATCATCCCAGAATCAGCTACAATTTCCGGTACTTTTAGAGCTTTTAGCAAGAAGAGCTTCAATGCAATCAGGGAAAGAATAGAAGAGGTTGTAAAAGGGCAGGCAGCTGTACATAGGTGCTCTGCTGAGATTGAATTCTTAGGAAATGAACACCCTAGCATCCCCCCTACTATAAACGATGAGAGAATTTACGAACAAGCAAGACGCATCTCAACCAAAATAGTAGGAAAAGAAAACACAAAATTAGCTCCTACCTTCATGGGGAGTGAGGATTTTGCTTTCTACTTGGATAAGGTACCTGGGTCAATGCTATTTTTAGGCACAGGAAATGAAAAGAAAGGAGCCATATATGCGCCACATAGTCCTTACTTTTTCATTGATGAGGATGTGCTTCCTATTGGTTCTGCAATACATGCAGCATTTGCCCATTCTTACCTAGCAGATTCAACTGGGAAGTTGCACCTTCATATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
M20_dimer Peptidase_M20

Protein Analysis

433

Amino Acids

47.12

Weight (kDa)

6.22

Isoelectric Point (pI)

36.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015613)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g35100
malus_domestica MD13G1086300.v1.1 MD16G1085700.v1.1
prunus_persica Prupe.1G260400_v2.0.a1
pyrus_communis pycom13g07450 pycom16g07400
rosa_chinensis RchiOBHm_Chr4g0444701
rosa_laevigata RLG00000005812
rosa_multiflora Rmu_sc0012443.1_g000011
rosa_roxburghii Rroxscaffold_5G00385200
rosa_rugosa Rorug04G0354600
rosa_samantha Rh4AG414700 Rh4BG426400 Rh4CG441300 Rh4DG422300
rosa_wichuraiana Rw4G035630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1120
AccB1I GGYRCC 1 cut(s) 1120
AccI GTMKAC 1 cut(s) 62
AccII CGCG 2 cut(s) 216, 507
AciI CCGC 1 cut(s) 507
AclWI GGATC 1 cut(s) 698
AcoI YGGCCR 3 cut(s) 108, 273, 397
AcsI RAATTY 4 cut(s) 188, 623, 959, 1011
AdeI CACNNNGTG 1 cut(s) 580
AfaI GTAC 4 cut(s) 441, 856, 936, 1122
AfiI CCNNNNNNNGG 2 cut(s) 567, 1086
AflIII ACRYGT 1 cut(s) 409
AjnI CCWGG 3 cut(s) 616, 780, 1123
AloI GAACNNNNNNTCC 2 cut(s) 462, 494
Alw21I GWGCWC 2 cut(s) 363, 947
Alw26I GTCTC 1 cut(s) 757
AlwI GGATC 1 cut(s) 698
Ama87I CYCGRG 1 cut(s) 754
AoxI GGCC 6 cut(s) 108, 248, 273, 397, 558, 614
ApeKI GCWGC 5 cut(s) 431, 680, 716, 929, 1241
ApoI RAATTY 4 cut(s) 188, 623, 959, 1011
Asp700I GAANNNNTTC 1 cut(s) 881
Asp718I GGTACC 1 cut(s) 1120
AspLEI GCGC 4 cut(s) 203, 216, 259, 1180
AspS9I GGNCC 1 cut(s) 4
AvaI CYCGRG 1 cut(s) 754
AvaII GGWCC 1 cut(s) 4
BaeI ACNNNNGTAYC 2 cut(s) 1112, 1145
BalI TGGCCA 3 cut(s) 110, 275, 399
BanI GGYRCC 1 cut(s) 1120
Bbv12I GWGCWC 2 cut(s) 363, 947
BbvCI CCTCAGC 1 cut(s) 498
BbvI GCAGC 5 cut(s) 418, 692, 728, 941, 1253
BccI CCATC 1 cut(s) 569
BciT130I CCWGG 3 cut(s) 618, 782, 1125
BclI TGATCA 1 cut(s) 528
BcoDI GTCTC 1 cut(s) 757
BfaI CTAG 3 cut(s) 629, 981, 1262
BfmI CTRYAG 1 cut(s) 792
BisI GCNGC 6 cut(s) 432, 508, 681, 717, 930, 1242
BlsI GCNGC 6 cut(s) 433, 509, 682, 718, 931, 1243
BmcAI AGTACT 1 cut(s) 441
Bme1390I CCNGG 3 cut(s) 618, 782, 1125
Bme18I GGWCC 1 cut(s) 4
BmeT110I CYCGRG 1 cut(s) 754
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 2 cut(s) 1122, 1168
BmrFI CCNGG 3 cut(s) 618, 782, 1125
BmrI ACTGGG 1 cut(s) 1284
BmsI GCATC 6 cut(s) 316, 617, 728, 734, 993, 1041
BmuI ACTGGG 1 cut(s) 1284
BpmI CTGGAG 1 cut(s) 639
Bpu10I CCTNAGC 1 cut(s) 498
BsaAI YACGTR 1 cut(s) 577
BsaJI CCNNGG 2 cut(s) 780, 1124
BsaWI WCCGGW 1 cut(s) 851
Bsc4I CCNNNNNNNGG 2 cut(s) 567, 1086
Bse1I ACTGG 2 cut(s) 268, 1279
Bse3DI GCAATG 1 cut(s) 33
BseBI CCWGG 3 cut(s) 618, 782, 1125
BseDI CCNNGG 2 cut(s) 780, 1124
BseGI GGATG 5 cut(s) 141, 331, 828, 984, 1216
BseLI CCNNNNNNNGG 2 cut(s) 567, 1086
BseMI GCAATG 1 cut(s) 33
BseMII CTCAG 3 cut(s) 302, 489, 942
BseNI ACTGG 2 cut(s) 268, 1279
BseRI GAGGAG 2 cut(s) 163, 285
BseXI GCAGC 5 cut(s) 418, 692, 728, 941, 1253
Bsh1236I CGCG 2 cut(s) 216, 507
BshFI GGCC 6 cut(s) 110, 250, 275, 399, 560, 616
BshNI GGYRCC 1 cut(s) 1120
BsiHKAI GWGCWC 2 cut(s) 363, 947
BsiHKCI CYCGRG 1 cut(s) 754
BsiSI CCGG 1 cut(s) 852
BslFI GGGAC 1 cut(s) 826
BslI CCNNNNNNNGG 2 cut(s) 567, 1086
BsmAI GTCTC 1 cut(s) 757
BsmBI CGTCTC 1 cut(s) 757
BsmFI GGGAC 1 cut(s) 826
BsnI GGCC 6 cut(s) 110, 250, 275, 399, 560, 616
BsoBI CYCGRG 1 cut(s) 754
Bsp1286I GDGCHC 2 cut(s) 363, 947
Bsp1407I TGTACA 1 cut(s) 934
Bsp143I GATC 2 cut(s) 528, 703
BspACI CCGC 1 cut(s) 507
BspANI GGCC 6 cut(s) 110, 250, 275, 399, 560, 616
BspCNI CTCAG 3 cut(s) 303, 490, 943
BspFNI CGCG 2 cut(s) 216, 507
BspLI GGNNCC 2 cut(s) 1122, 1168
BspPI GGATC 1 cut(s) 698
BspQI GCTCTTC 1 cut(s) 872
BspT107I GGYRCC 1 cut(s) 1120
BsrDI GCAATG 1 cut(s) 33
BsrGI TGTACA 1 cut(s) 934
BsrI ACTGG 2 cut(s) 268, 1279
BssECI CCNNGG 2 cut(s) 780, 1124
BssMI GATC 2 cut(s) 528, 703
Bst2UI CCWGG 3 cut(s) 618, 782, 1125
Bst4CI ACNGT 2 cut(s) 127, 475
Bst6I CTCTTC 2 cut(s) 872, 903
BstAPI GCANNNNNTGC 4 cut(s) 413, 580, 1238, 1247
BstAUI TGTACA 1 cut(s) 934
BstBAI YACGTR 1 cut(s) 577
BstC8I GCNNGC 5 cut(s) 108, 218, 390, 740, 927
BstDEI CTNAG 4 cut(s) 311, 498, 951, 964
BstF5I GGATG 5 cut(s) 141, 331, 828, 984, 1216
BstFNI CGCG 2 cut(s) 216, 507
BstHHI GCGC 4 cut(s) 203, 216, 259, 1180
BstKTI GATC 2 cut(s) 531, 706
BstMAI GTCTC 1 cut(s) 757
BstMBI GATC 2 cut(s) 528, 703
BstMWI GCNNNNNNNGC 9 cut(s) 256, 314, 413, 580, 722, 879, 1175, 1238, 1247
BstNI CCWGG 3 cut(s) 618, 782, 1125
BstNSI RCATGY 3 cut(s) 392, 413, 1241
BstSCI CCNGG 3 cut(s) 616, 780, 1123
BstSFI CTRYAG 1 cut(s) 792
BstUI CGCG 2 cut(s) 216, 507
BstV1I GCAGC 5 cut(s) 418, 692, 728, 941, 1253
BsuRI GGCC 6 cut(s) 110, 250, 275, 399, 560, 616
BtsCI GGATG 5 cut(s) 141, 331, 828, 984, 1216
BtsIMutI CAGTG 1 cut(s) 735
Cac8I GCNNGC 5 cut(s) 108, 218, 390, 740, 927
CfoI GCGC 4 cut(s) 203, 216, 259, 1180
Cfr13I GGNCC 1 cut(s) 4
CseI GACGC 2 cut(s) 413, 1038
Csp6I GTAC 4 cut(s) 440, 855, 935, 1121
CviQI GTAC 4 cut(s) 440, 855, 935, 1121
DdeI CTNAG 4 cut(s) 311, 498, 951, 964
DpnI GATC 2 cut(s) 530, 705
DpnII GATC 2 cut(s) 528, 703
DraIII CACNNNGTG 1 cut(s) 580
EaeI YGGCCR 3 cut(s) 108, 273, 397
Eam1104I CTCTTC 2 cut(s) 872, 903
EarI CTCTTC 2 cut(s) 872, 903
Eco147I AGGCCT 1 cut(s) 616
Eco32I GATATC 1 cut(s) 590
Eco47I GGWCC 1 cut(s) 4
Eco88I CYCGRG 1 cut(s) 754
EcoRI GAATTC 2 cut(s) 188, 959
EcoRII CCWGG 3 cut(s) 616, 780, 1123
EcoRV GATATC 1 cut(s) 590
EcoT22I ATGCAT 1 cut(s) 390
Esp3I CGTCTC 1 cut(s) 757
FalI AAGNNNNNCTT 4 cut(s) 528, 560, 866, 898
FaqI GGGAC 1 cut(s) 826
FauNDI CATATG 1 cut(s) 181
FbaI TGATCA 1 cut(s) 528
FblI GTMKAC 1 cut(s) 62
Fnu4HI GCNGC 6 cut(s) 432, 508, 681, 717, 930, 1242
FokI GGATG 5 cut(s) 148, 338, 815, 971, 1223
Fsp4HI GCNGC 6 cut(s) 432, 508, 681, 717, 930, 1242
FspBI CTAG 3 cut(s) 629, 981, 1262
GlaI GCGC 4 cut(s) 202, 215, 258, 1179
GluI GCNGC 6 cut(s) 432, 508, 681, 717, 930, 1242
GsuI CTGGAG 1 cut(s) 639
HaeIII GGCC 6 cut(s) 110, 250, 275, 399, 560, 616
HapII CCGG 1 cut(s) 852
HgaI GACGC 2 cut(s) 413, 1038
HhaI GCGC 4 cut(s) 203, 216, 259, 1180
Hin6I GCGC 4 cut(s) 201, 214, 257, 1178
HinP1I GCGC 4 cut(s) 201, 214, 257, 1178
HinfI GANTC 5 cut(s) 658, 776, 802, 836, 1268
HpaII CCGG 1 cut(s) 852
Hpy166II GTNNAC 2 cut(s) 63, 376
Hpy188I TCNGA 2 cut(s) 312, 1298
Hpy188III TCNNGA 4 cut(s) 74, 341, 754, 756
Hpy8I GTNNAC 2 cut(s) 63, 376
Hpy99I CGWCG 1 cut(s) 407
HpyAV CCTTC 5 cut(s) 17, 514, 530, 1090, 1298
HpyCH4III ACNGT 2 cut(s) 127, 475
HpyCH4IV ACGT 1 cut(s) 576
HpyF10VI GCNNNNNNNGC 9 cut(s) 256, 314, 413, 580, 722, 879, 1175, 1238, 1247
HpyF3I CTNAG 4 cut(s) 311, 498, 951, 964
HpySE526I ACGT 1 cut(s) 576
HspAI GCGC 4 cut(s) 201, 214, 257, 1178
KpnI GGTACC 1 cut(s) 1124
Ksp22I TGATCA 1 cut(s) 528
Kzo9I GATC 2 cut(s) 528, 703
LguI GCTCTTC 1 cut(s) 872
LmnI GCTCC 4 cut(s) 358, 642, 1078, 1166
Lsp1109I GCAGC 5 cut(s) 418, 692, 728, 941, 1253
LweI GCATC 6 cut(s) 316, 617, 728, 734, 993, 1041
MaeI CTAG 3 cut(s) 629, 981, 1262
MaeII ACGT 1 cut(s) 576
MalI GATC 2 cut(s) 530, 705
MboI GATC 2 cut(s) 528, 703
MboII GAAGA 4 cut(s) 151, 197, 889, 920
MfeI CAATTG 2 cut(s) 449, 708
MhlI GDGCHC 2 cut(s) 363, 947
MlsI TGGCCA 3 cut(s) 110, 275, 399
MluNI TGGCCA 3 cut(s) 110, 275, 399
MnlI CCTC 9 cut(s) 141, 306, 493, 550, 699, 754, 904, 1087, 1201
Mox20I TGGCCA 3 cut(s) 110, 275, 399
Mph1103I ATGCAT 1 cut(s) 390
MroXI GAANNNNTTC 1 cut(s) 881
MscI TGGCCA 3 cut(s) 110, 275, 399
MseI TTAA 1 cut(s) 822
MslI CAYNNNNRTG 3 cut(s) 244, 264, 393
Msp20I TGGCCA 3 cut(s) 110, 275, 399
MspA1I CMGCKG 2 cut(s) 497, 932
MspI CCGG 1 cut(s) 852
MspR9I CCNGG 3 cut(s) 618, 782, 1125
MunI CAATTG 2 cut(s) 449, 708
MvaI CCWGG 3 cut(s) 618, 782, 1125
MvnI CGCG 2 cut(s) 216, 507
MwoI GCNNNNNNNGC 9 cut(s) 256, 314, 413, 580, 722, 879, 1175, 1238, 1247
NdeI CATATG 1 cut(s) 181
NdeII GATC 2 cut(s) 528, 703
NlaIV GGNNCC 2 cut(s) 1122, 1168
NsiI ATGCAT 1 cut(s) 390
NspI RCATGY 3 cut(s) 392, 413, 1241
PaeI GCATGC 1 cut(s) 392
PaeR7I CTCGAG 1 cut(s) 754
PceI AGGCCT 1 cut(s) 616
PciI ACATGT 1 cut(s) 409
PciSI GCTCTTC 1 cut(s) 872
PdmI GAANNNNTTC 1 cut(s) 881
PfeI GAWTC 5 cut(s) 658, 776, 802, 836, 1268
PkrI GCNGC 6 cut(s) 433, 509, 682, 718, 931, 1243
Ppu21I YACGTR 1 cut(s) 577
PscI ACATGT 1 cut(s) 409
Psp6I CCWGG 3 cut(s) 616, 780, 1123
PspGI CCWGG 3 cut(s) 616, 780, 1123
PspN4I GGNNCC 2 cut(s) 1122, 1168
PspPI GGNCC 1 cut(s) 4
PvuII CAGCTG 2 cut(s) 497, 932
RsaI GTAC 4 cut(s) 441, 856, 936, 1122
RsaNI GTAC 4 cut(s) 440, 855, 935, 1121
RseI CAYNNNNRTG 3 cut(s) 244, 264, 393
SapI GCTCTTC 1 cut(s) 872
SaqAI TTAA 1 cut(s) 822
SatI GCNGC 6 cut(s) 432, 508, 681, 717, 930, 1242
Sau3AI GATC 2 cut(s) 528, 703
Sau96I GGNCC 1 cut(s) 4
ScaI AGTACT 1 cut(s) 441
ScrFI CCNGG 3 cut(s) 618, 782, 1125
SduI GDGCHC 2 cut(s) 363, 947
SfaNI GCATC 6 cut(s) 316, 617, 728, 734, 993, 1041
SfcI CTRYAG 1 cut(s) 792
Sfr274I CTCGAG 1 cut(s) 754
SinI GGWCC 1 cut(s) 4
SlaI CTCGAG 1 cut(s) 754
SmiMI CAYNNNNRTG 3 cut(s) 244, 264, 393
SmlI CTYRAG 1 cut(s) 754
SmoI CTYRAG 1 cut(s) 754
SphI GCATGC 1 cut(s) 392
SseBI AGGCCT 1 cut(s) 616
SsiI CCGC 1 cut(s) 507
SspMI CTAG 3 cut(s) 629, 981, 1262
StuI AGGCCT 1 cut(s) 616
StyD4I CCNGG 3 cut(s) 616, 780, 1123
TaaI ACNGT 2 cut(s) 127, 475
TaiI ACGT 1 cut(s) 579
TaqI TCGA 1 cut(s) 755
TatI WGTACW 2 cut(s) 439, 934
TauI GCSGC 1 cut(s) 510
TfiI GAWTC 5 cut(s) 658, 776, 802, 836, 1268
Tru1I TTAA 1 cut(s) 822
Tru9I TTAA 1 cut(s) 822
TscAI CASTG 1 cut(s) 735
TseI GCWGC 5 cut(s) 431, 680, 716, 929, 1241
TspGWI ACGGA 1 cut(s) 393
TspRI CASTG 1 cut(s) 735
VpaK11BI GGWCC 1 cut(s) 4
XapI RAATTY 4 cut(s) 188, 623, 959, 1011
XceI RCATGY 3 cut(s) 392, 413, 1241
XcmI CCANNNNNNNNNTGG 1 cut(s) 343
XhoI CTCGAG 1 cut(s) 754
XmiI GTMKAC 1 cut(s) 62
XmnI GAANNNNTTC 1 cut(s) 881
XspI CTAG 3 cut(s) 629, 981, 1262
ZrmI AGTACT 1 cut(s) 441
Zsp2I ATGCAT 1 cut(s) 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.