MD16G1085700.v1.1

IAA-amino acid hydrolase ILR1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0015613
Physical Location & Seq
Forward (+)
5994062 .. 5996080
2019 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1085700.v1.1.491

Sequence Viewer

Length: 1347 bp
ATGGACTTTCTTCTTCTGCAACTTTTGTTCCTAATAATCTCCCTTGCCATGCCCTTGTGCTTTTCTCTGAAACTCCAAATTAACCCTACCGATCATCTCAACTTATCCACCACATATCAGACTTATTCAGTGAAACACCAAATAGTTAGGCTGGCCAACGAACCCAGCACAGTGAATTGGATGAAAAACGTGAGAAGGGAAATGCACGAAAACCCAGAACTTTCATATGAAGAAATCGAAACAAGTGCACTCATTCGCCGCGAGCTTGATAAACTCGGTGTTGCTTATAAATGGCCTGTGGCTAAAACTGGTGTTGTTGCCACGATTGGGTCTGGCTCTCCTCCATTTGTTGCTCTAAGAGCTGATATGGATGCTTTGCCTATTCAGGAAATGGTGGACTGGGAGAACAAGAGCAAAGTGGAGGGAAAAATGCATGCTTGCGGCCACGACGGCCACGTCGCCATGCTTCTTGGCGCTGCAAAAGTACTAGAACAACTGCAAGACACATTACAGGGAACCGTTGTACTTATATTTCAACCGGCTGAAGAACGTGGTGCAGGCGCAAAGGACATGATTGAAGAAGGGGTGCTTGATAATGTGGAGGCAGTTTTTGGGATGCACGTAGCGCCTATATATCCGACTGGCGTTGTTGCATTGAGGCCTGGAGAATTTCTAGCCGGTTGTGGGAGCTTCAAAGCGAAAATTCACGGGAAAGGGGGTCATGCAGCAATTCCTCAGCAATCCATTGATCCGATTTTGGCAGCATCAACATCTGTAATTAGTTTGCAGAACATCATCTCGAGAGAAACAGACCCTCTAGATTCCCAGGTGGTTTCTGTTGCTGTGATTAAAGCGGGGACTTCCTTCAATGTCATCCCGCAGTCAGCTACAATTTCTGGTACGTTTAGAGCTTTTAGCAAGAAGAACCTCAATGGACTCATGAAAAGAATCGAAGAGGTTGTAAAAGGGCAGGCAGCCGTGCACCGGTGCTCCGCTGAGGTCGAATTCTTTGGGAAGGAACATCCTACCATCCTTCCTACTGTAAACGACGAGAGAATTTATGAACAAGTAAGGCGAATCTCGAGCGAAATAGTAGGAGAAGAAAACATAAAATTAGCTCATACCTTCATGGGGAGTGAGGATTTTGCTTTTTACTTAGACAAGGTGCCTGGATTCATGCTGTTTTTAGGCGCAAGAAATGAGAAGATAGGAGCCATACATGCACCGCACAGTCCTCACTACATCCTCGATGAGGATGTGCTTCCCATTGGGGCTGCCATACATGCAGCTTTTGCCCATTCTTTTCTATCAGATTCAACTGGGAAGTTGCACCTTCATAATTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
M20_dimer Peptidase_M20

Protein Analysis

449

Amino Acids

49.07

Weight (kDa)

5.95

Isoelectric Point (pI)

47.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M20 PF01546 118 - 432 3.6e-48 Peptidase family M20/M25/M40
M20_dimer PF07687 227 - 325 3.2e-11 Peptidase dimerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015613)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g35100
malus_domestica MD13G1086300.v1.1 MD16G1085700.v1.1
prunus_persica Prupe.1G260400_v2.0.a1
pyrus_communis pycom13g07450 pycom16g07400
rosa_chinensis RchiOBHm_Chr4g0444701
rosa_laevigata RLG00000005812
rosa_multiflora Rmu_sc0012443.1_g000011
rosa_roxburghii Rroxscaffold_5G00385200
rosa_rugosa Rorug04G0354600
rosa_samantha Rh4AG414700 Rh4BG426400 Rh4CG441300 Rh4DG422300
rosa_wichuraiana Rw4G035630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 288
AasI GACNNNNNNGTC 1 cut(s) 455
AccB1I GGYRCC 1 cut(s) 1165
AccII CGCG 1 cut(s) 261
AciI CCGC 6 cut(s) 259, 441, 854, 878, 993, 1226
AclWI GGATC 1 cut(s) 743
AcoI YGGCCR 3 cut(s) 153, 442, 451
AcsI RAATTY 4 cut(s) 668, 702, 1004, 1056
AcuI CTGAAG 1 cut(s) 564
AfaI GTAC 3 cut(s) 486, 525, 901
AfiI CCNNNNNNNGG 5 cut(s) 327, 684, 984, 1131, 1252
AgeI ACCGGT 1 cut(s) 984
AgsI TTSAA 5 cut(s) 536, 578, 694, 868, 1317
AjiI CACGTC 1 cut(s) 457
AjnI CCWGG 3 cut(s) 661, 825, 1168
AluBI AGCT 7 cut(s) 265, 362, 690, 887, 911, 1118, 1289
AluI AGCT 7 cut(s) 265, 362, 690, 887, 911, 1118, 1289
Alw21I GWGCWC 3 cut(s) 250, 984, 992
Alw44I GTGCAC 2 cut(s) 246, 980
AlwI GGATC 1 cut(s) 743
Ama87I CYCGRG 2 cut(s) 799, 1081
AoxI GGCC 5 cut(s) 153, 293, 442, 451, 659
ApaLI GTGCAC 2 cut(s) 246, 980
ApeKI GCWGC 6 cut(s) 476, 725, 761, 974, 1274, 1286
ApoI RAATTY 4 cut(s) 668, 702, 1004, 1056
AsiGI ACCGGT 1 cut(s) 984
AspLEI GCGC 4 cut(s) 476, 563, 628, 1193
AvaI CYCGRG 2 cut(s) 799, 1081
BaeGI GKGCMC 2 cut(s) 250, 984
BalI TGGCCA 1 cut(s) 155
BanI GGYRCC 1 cut(s) 1165
Bbv12I GWGCWC 3 cut(s) 250, 984, 992
BbvCI CCTCAGC 2 cut(s) 735, 996
BbvI GCAGC 6 cut(s) 463, 737, 773, 986, 1261, 1298
BccI CCATC 1 cut(s) 1037
BceAI ACGGC 2 cut(s) 466, 962
BcgI CGANNNNNNTGC 2 cut(s) 227, 261
BciT130I CCWGG 3 cut(s) 663, 827, 1170
BfaI CTAG 3 cut(s) 488, 674, 818
BfoI RGCGCY 2 cut(s) 477, 629
BglI GCCNNNNNGGC 1 cut(s) 450
BisI GCNGC 8 cut(s) 259, 442, 477, 726, 762, 975, 1275, 1287
BlsI GCNGC 8 cut(s) 260, 443, 478, 727, 763, 976, 1276, 1288
BmcAI AGTACT 1 cut(s) 486
Bme1390I CCNGG 3 cut(s) 663, 827, 1170
BmeT110I CYCGRG 2 cut(s) 799, 1081
BmgBI CACGTC 1 cut(s) 457
BmiI GGNNCC 3 cut(s) 517, 1167, 1213
BmrFI CCNGG 3 cut(s) 663, 827, 1170
BmrI ACTGGG 2 cut(s) 409, 1329
BmsI GCATC 3 cut(s) 361, 606, 773
BmuI ACTGGG 2 cut(s) 409, 1329
BpmI CTGGAG 1 cut(s) 684
Bpu10I CCTNAGC 2 cut(s) 735, 996
BsaAI YACGTR 1 cut(s) 622
BsaJI CCNNGG 1 cut(s) 825
BsaWI WCCGGW 1 cut(s) 984
BsaXI ACNNNNNCTCC 2 cut(s) 974, 1004
Bsc4I CCNNNNNNNGG 5 cut(s) 327, 684, 984, 1131, 1252
Bse118I RCCGGY 3 cut(s) 538, 677, 984
Bse1I ACTGG 4 cut(s) 313, 404, 646, 1324
BseBI CCWGG 3 cut(s) 663, 827, 1170
BseDI CCNNGG 1 cut(s) 825
BseGI GGATG 8 cut(s) 186, 376, 621, 873, 1021, 1029, 1242, 1261
BseLI CCNNNNNNNGG 5 cut(s) 327, 684, 984, 1131, 1252
BseMII CTCAG 2 cut(s) 749, 987
BseNI ACTGG 4 cut(s) 313, 404, 646, 1324
BseRI GAGGAG 1 cut(s) 330
BseSI GKGCMC 2 cut(s) 250, 984
BseXI GCAGC 6 cut(s) 463, 737, 773, 986, 1261, 1298
BseYI CCCAGC 1 cut(s) 164
BsgI GTGCAG 1 cut(s) 576
Bsh1236I CGCG 1 cut(s) 261
BshFI GGCC 5 cut(s) 155, 295, 444, 453, 661
BshNI GGYRCC 1 cut(s) 1165
BshTI ACCGGT 1 cut(s) 984
BsiHKAI GWGCWC 3 cut(s) 250, 984, 992
BsiHKCI CYCGRG 2 cut(s) 799, 1081
BsiSI CCGG 3 cut(s) 539, 678, 985
BslFI GGGAC 1 cut(s) 871
BslI CCNNNNNNNGG 5 cut(s) 327, 684, 984, 1131, 1252
BsmFI GGGAC 1 cut(s) 871
BsnI GGCC 5 cut(s) 155, 295, 444, 453, 661
BsoBI CYCGRG 2 cut(s) 799, 1081
Bsp1286I GDGCHC 3 cut(s) 250, 984, 992
Bsp143I GATC 2 cut(s) 91, 748
BspACI CCGC 6 cut(s) 259, 441, 854, 878, 993, 1226
BspANI GGCC 5 cut(s) 155, 295, 444, 453, 661
BspCNI CTCAG 2 cut(s) 748, 988
BspFNI CGCG 1 cut(s) 261
BspHI TCATGA 1 cut(s) 939
BspLI GGNNCC 3 cut(s) 517, 1167, 1213
BspPI GGATC 1 cut(s) 743
BspT107I GGYRCC 1 cut(s) 1165
BsrFI RCCGGY 3 cut(s) 538, 677, 984
BsrI ACTGG 4 cut(s) 313, 404, 646, 1324
BssAI RCCGGY 3 cut(s) 538, 677, 984
BssECI CCNNGG 1 cut(s) 825
BssMI GATC 2 cut(s) 91, 748
Bst2UI CCWGG 3 cut(s) 663, 827, 1170
Bst4CI ACNGT 4 cut(s) 172, 520, 1042, 1232
Bst6I CTCTTC 1 cut(s) 948
BstAPI GCANNNNNTGC 1 cut(s) 1292
BstBAI YACGTR 1 cut(s) 622
BstC8I GCNNGC 6 cut(s) 153, 263, 435, 439, 559, 972
BstDEI CTNAG 4 cut(s) 356, 735, 996, 1156
BstENI CCTNNNNNAGG 1 cut(s) 1250
BstF5I GGATG 8 cut(s) 186, 376, 621, 873, 1021, 1029, 1242, 1261
BstFNI CGCG 1 cut(s) 261
BstH2I RGCGCY 2 cut(s) 477, 629
BstHHI GCGC 4 cut(s) 476, 563, 628, 1193
BstKTI GATC 2 cut(s) 94, 751
BstMBI GATC 2 cut(s) 91, 748
BstMWI GCNNNNNNNGC 6 cut(s) 359, 450, 625, 1220, 1283, 1292
BstNI CCWGG 3 cut(s) 663, 827, 1170
BstNSI RCATGY 3 cut(s) 437, 1223, 1286
BstSCI CCNGG 3 cut(s) 661, 825, 1168
BstSLI GKGCMC 2 cut(s) 250, 984
BstUI CGCG 1 cut(s) 261
BstV1I GCAGC 6 cut(s) 463, 737, 773, 986, 1261, 1298
BsuRI GGCC 5 cut(s) 155, 295, 444, 453, 661
BtrI CACGTC 1 cut(s) 457
BtsCI GGATG 8 cut(s) 186, 376, 621, 873, 1021, 1029, 1242, 1261
BtsIMutI CAGTG 2 cut(s) 135, 177
Cac8I GCNNGC 6 cut(s) 153, 263, 435, 439, 559, 972
CciI TCATGA 1 cut(s) 939
CfoI GCGC 4 cut(s) 476, 563, 628, 1193
Cfr10I RCCGGY 3 cut(s) 538, 677, 984
Csp6I GTAC 3 cut(s) 485, 524, 900
CspAI ACCGGT 1 cut(s) 984
CviQI GTAC 3 cut(s) 485, 524, 900
DdeI CTNAG 4 cut(s) 356, 735, 996, 1156
DpnI GATC 2 cut(s) 93, 750
DpnII GATC 2 cut(s) 91, 748
DrdI GACNNNNNNGTC 1 cut(s) 455
DseDI GACNNNNNNGTC 1 cut(s) 455
EaeI YGGCCR 3 cut(s) 153, 442, 451
Eam1104I CTCTTC 1 cut(s) 948
EarI CTCTTC 1 cut(s) 948
Eco147I AGGCCT 1 cut(s) 661
Eco57I CTGAAG 1 cut(s) 564
Eco88I CYCGRG 2 cut(s) 799, 1081
EcoNI CCTNNNNNAGG 1 cut(s) 1250
EcoRI GAATTC 1 cut(s) 1004
EcoRII CCWGG 3 cut(s) 661, 825, 1168
EcoT22I ATGCAT 1 cut(s) 435
FalI AAGNNNNNCTT 2 cut(s) 573, 605
FaqI GGGAC 1 cut(s) 871
FauI CCCGC 2 cut(s) 847, 885
FauNDI CATATG 1 cut(s) 226
Fnu4HI GCNGC 8 cut(s) 259, 442, 477, 726, 762, 975, 1275, 1287
FokI GGATG 8 cut(s) 193, 383, 628, 860, 1008, 1016, 1229, 1268
Fsp4HI GCNGC 8 cut(s) 259, 442, 477, 726, 762, 975, 1275, 1287
FspBI CTAG 3 cut(s) 488, 674, 818
GlaI GCGC 4 cut(s) 475, 562, 627, 1192
GluI GCNGC 8 cut(s) 259, 442, 477, 726, 762, 975, 1275, 1287
GsaI CCCAGC 1 cut(s) 168
GsuI CTGGAG 1 cut(s) 684
HaeII RGCGCY 2 cut(s) 477, 629
HaeIII GGCC 5 cut(s) 155, 295, 444, 453, 661
HapII CCGG 3 cut(s) 539, 678, 985
HhaI GCGC 4 cut(s) 476, 563, 628, 1193
Hin6I GCGC 4 cut(s) 474, 561, 626, 1191
HinP1I GCGC 4 cut(s) 474, 561, 626, 1191
HinfI GANTC 6 cut(s) 821, 936, 948, 1077, 1173, 1313
HpaII CCGG 3 cut(s) 539, 678, 985
Hpy166II GTNNAC 4 cut(s) 248, 397, 982, 1045
Hpy188I TCNGA 5 cut(s) 69, 120, 639, 753, 1312
Hpy188III TCNNGA 6 cut(s) 386, 799, 801, 818, 940, 1081
Hpy8I GTNNAC 4 cut(s) 248, 397, 982, 1045
Hpy99I CGWCG 3 cut(s) 452, 461, 1052
HpyAV CCTTC 7 cut(s) 189, 575, 874, 1009, 1043, 1135, 1343
HpyCH4III ACNGT 4 cut(s) 172, 520, 1042, 1232
HpyCH4IV ACGT 5 cut(s) 189, 456, 550, 621, 902
HpyF10VI GCNNNNNNNGC 6 cut(s) 359, 450, 625, 1220, 1283, 1292
HpyF3I CTNAG 4 cut(s) 356, 735, 996, 1156
HpySE526I ACGT 5 cut(s) 189, 456, 550, 621, 902
HspAI GCGC 4 cut(s) 474, 561, 626, 1191
Kzo9I GATC 2 cut(s) 91, 748
LmnI GCTCC 3 cut(s) 687, 995, 1211
Lsp1109I GCAGC 6 cut(s) 463, 737, 773, 986, 1261, 1298
LweI GCATC 3 cut(s) 361, 606, 773
MaeI CTAG 3 cut(s) 488, 674, 818
MaeII ACGT 5 cut(s) 189, 456, 550, 621, 902
MalI GATC 2 cut(s) 93, 750
MboI GATC 2 cut(s) 91, 748
MboII GAAGA 8 cut(s) 5, 242, 557, 590, 934, 965, 1112, 1216
MhlI GDGCHC 3 cut(s) 250, 984, 992
MlsI TGGCCA 1 cut(s) 155
MluNI TGGCCA 1 cut(s) 155
MlyI GAGTC 1 cut(s) 930
MmeI TCCRAC 1 cut(s) 662
Mox20I TGGCCA 1 cut(s) 155
Mph1103I ATGCAT 1 cut(s) 435
MscI TGGCCA 1 cut(s) 155
MseI TTAA 2 cut(s) 81, 849
Msp20I TGGCCA 1 cut(s) 155
MspA1I CMGCKG 1 cut(s) 995
MspI CCGG 3 cut(s) 539, 678, 985
MspR9I CCNGG 3 cut(s) 663, 827, 1170
MvaI CCWGG 3 cut(s) 663, 827, 1170
MvnI CGCG 1 cut(s) 261
MwoI GCNNNNNNNGC 6 cut(s) 359, 450, 625, 1220, 1283, 1292
NdeI CATATG 1 cut(s) 226
NdeII GATC 2 cut(s) 91, 748
NlaIV GGNNCC 3 cut(s) 517, 1167, 1213
NsiI ATGCAT 1 cut(s) 435
NspI RCATGY 3 cut(s) 437, 1223, 1286
PaeI GCATGC 1 cut(s) 437
PaeR7I CTCGAG 2 cut(s) 799, 1081
PagI TCATGA 1 cut(s) 939
PceI AGGCCT 1 cut(s) 661
PcsI WCGNNNNNNNCGW 1 cut(s) 453
PfeI GAWTC 5 cut(s) 821, 948, 1077, 1173, 1313
PinAI ACCGGT 1 cut(s) 984
PkrI GCNGC 8 cut(s) 260, 443, 478, 727, 763, 976, 1276, 1288
PleI GAGTC 1 cut(s) 930
PpsI GAGTC 1 cut(s) 930
Ppu21I YACGTR 1 cut(s) 622
PsiI TTATAA 1 cut(s) 288
Psp6I CCWGG 3 cut(s) 661, 825, 1168
PspFI CCCAGC 1 cut(s) 164
PspGI CCWGG 3 cut(s) 661, 825, 1168
PspN4I GGNNCC 3 cut(s) 517, 1167, 1213
RsaI GTAC 3 cut(s) 486, 525, 901
RsaNI GTAC 3 cut(s) 485, 524, 900
SaqAI TTAA 2 cut(s) 81, 849
SatI GCNGC 8 cut(s) 259, 442, 477, 726, 762, 975, 1275, 1287
Sau3AI GATC 2 cut(s) 91, 748
ScaI AGTACT 1 cut(s) 486
SchI GAGTC 1 cut(s) 930
ScrFI CCNGG 3 cut(s) 663, 827, 1170
SduI GDGCHC 3 cut(s) 250, 984, 992
SfaNI GCATC 3 cut(s) 361, 606, 773
SfiI GGCCNNNNNGGCC 1 cut(s) 450
Sfr274I CTCGAG 2 cut(s) 799, 1081
SgrAI CRCCGGYG 1 cut(s) 984
SlaI CTCGAG 2 cut(s) 799, 1081
SmlI CTYRAG 2 cut(s) 799, 1081
SmoI CTYRAG 2 cut(s) 799, 1081
SphI GCATGC 1 cut(s) 437
SseBI AGGCCT 1 cut(s) 661
SsiI CCGC 6 cut(s) 259, 441, 854, 878, 993, 1226
SspMI CTAG 3 cut(s) 488, 674, 818
StuI AGGCCT 1 cut(s) 661
StyD4I CCNGG 3 cut(s) 661, 825, 1168
TaaI ACNGT 4 cut(s) 172, 520, 1042, 1232
TaiI ACGT 5 cut(s) 192, 459, 553, 624, 905
TaqI TCGA 6 cut(s) 237, 800, 951, 1002, 1082, 1248
TatI WGTACW 2 cut(s) 484, 523
TauI GCSGC 2 cut(s) 261, 444
TfiI GAWTC 5 cut(s) 821, 948, 1077, 1173, 1313
Tru1I TTAA 2 cut(s) 81, 849
Tru9I TTAA 2 cut(s) 81, 849
TscAI CASTG 2 cut(s) 135, 177
TseI GCWGC 6 cut(s) 476, 725, 761, 974, 1274, 1286
TspDTI ATGAA 8 cut(s) 197, 213, 243, 956, 1077, 1117, 1165, 1325
TspRI CASTG 2 cut(s) 135, 177
VneI GTGCAC 2 cut(s) 246, 980
XagI CCTNNNNNAGG 1 cut(s) 1250
XapI RAATTY 4 cut(s) 668, 702, 1004, 1056
XbaI TCTAGA 1 cut(s) 817
XceI RCATGY 3 cut(s) 437, 1223, 1286
XhoI CTCGAG 2 cut(s) 799, 1081
XspI CTAG 3 cut(s) 488, 674, 818
ZrmI AGTACT 1 cut(s) 486
Zsp2I ATGCAT 1 cut(s) 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.