Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0015613

Physical Location & Seq

Genomic Coordinates Reverse (-)

62470624 .. 62473027

Gene Structure 2404 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G035630.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1326 bp

ATGGACCTTCTGCAACTTTGGTTCCTAATCTCCATTGCCACACCCTTGTGCTTTTCTCTCAACCTCCAAGCACCTGCTCTTGATATTTCCACCACTTACGAGAATCACTCACTGAAGGATGAGATAGTGAGGCTGGCAAAGGAACCCAGCACAGCAAATTGGATGAAGGATGTGAGGAGAGAAATCCATGAAAACCCAGAACTTGCATATGAAGAAATCAAAACAAGTGCAGTCATTCGTCGCGAGCTTGACAGATTAGGTGTTTCGTATAGATGGCCTGTGGCGCGGACCGGTGTAGTGGCCACTATTGGCTCTGGCTCTCCTCCTTTTGTTGCTCTAAGAGCTGATATGGATGCTCTGCCTATGCAGGAATTGGTGGAATGGGAGCACAAGAGCAAAGTGGATGGGAAATTCCATGCCTGTGGGCATGATGCACATGTTGCAATGCTTCTTGGTGCTGCAAAGGTACTCAAACAAGTCGAAGATGAATTGCAGGGAACTGTTGTTCTCATATTCCAGCCGGCTGAGGAACGTGGGGAAGGCGCAAAGGACATGATCAAAGAAGGGGTGCTTGACAATGTAGAGGCCATACTTGGGATTCACATTGTGCATAGATATCCTAGTGGAGTTTTTGCATCAAGGCCCGGAGAATTTCTAGCTGGGTGTGGGAGCTTCAAAGCAAAGATTATTGGGAAAGGGGGTCATGCAGCCCTTCCCCAACAGTCCATTGATCCAATTTTGGCAGCATCGGCATCAGTAGTTAGCTTGCAAAGCATTGTTTCTAGAGAAATAGACCCTCTAGATTCCGGGGTGGTTTCTGTAGCTATGATCCAAGCAGGGTCTGCATTTAACGTCATCCCAGACTCAGCTACAATAGCTGGTACTTATAGAGCTTTTAGCAAGAAAAGCTTCAGTGCACTCAGCCAAAGAATAGAAGAGGTTGTAAAAGGGCAGGCAGCTGTACATAGGTGCTCTGCTGAGATTGAATTTTTAGGGGAAGGAAATCCAAACCTTCCCCCTACCATAAATGATGAGAGAATTTATGAACAAGTTCGAGGATTATCAAGTGAACTTGTTGGAAATGAAAACACTGAGTTAGCTCCTACCTTCATGGGGAGTGAGGATTTTGCTTTCTTCTTAGAAAAGGTACCTGGATCAATGTTCTTCCTAGGCACAAGAAATGAGAAGAAAGGAGCGACACATCCCCCACACAGTCCTTACTATTTCATTGATGAAGATATCTTTCCAATTGGGGCTGCATTACATGCTAAGTTTGCCTATTCGTTCCTATCGGATTCGGCTGAGAAGTTGCAACTCCATTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0008150 GO:0008152 GO:0009850 GO:0009987 GO:0010178 GO:0010179 GO:0010817 GO:0012505 GO:0016787 GO:0016810 GO:0042445 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0065007 GO:0065008

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

M20_dimer Peptidase_M20

Protein Analysis

441

Amino Acids

48.17

Weight (kDa)

5.37

Isoelectric Point (pI)

40.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M20	PF01546	112 - 426	5.7e-46	Peptidase family M20/M25/M40
M20_dimer	PF07687	221 - 319	2.1e-09	Peptidase dimerisation domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015613)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g35100
malus_domestica	MD13G1086300.v1.1 MD16G1085700.v1.1
prunus_persica	Prupe.1G260400_v2.0.a1
pyrus_communis	pycom13g07450 pycom16g07400
rosa_chinensis	RchiOBHm_Chr4g0444701
rosa_laevigata	RLG00000005812
rosa_multiflora	Rmu_sc0012443.1_g000011
rosa_roxburghii	Rroxscaffold_5G00385200
rosa_rugosa	Rorug04G0354600
rosa_samantha	Rh4AG414700 Rh4BG426400 Rh4CG441300 Rh4DG422300
rosa_wichuraiana	Rw4G035630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	82
Acc36I	ACCTGC	1 cut(s)	82
Acc65I	GGTACC	1 cut(s)	1147
AccB1I	GGYRCC	1 cut(s)	1147
AccII	CGCG	2 cut(s)	243, 286
AciI	CCGC	1 cut(s)	286
AclWI	GGATC	3 cut(s)	725, 823, 1162
AcoI	YGGCCR	1 cut(s)	300
AcsI	RAATTY	4 cut(s)	410, 650, 986, 1038
AcuI	CTGAAG	2 cut(s)	134, 895
AdeI	CACNNNGTG	1 cut(s)	607
AfaI	GTAC	4 cut(s)	468, 883, 963, 1149
AfiI	CCNNNNNNNGG	2 cut(s)	594, 1113
AflIII	ACRYGT	1 cut(s)	436
AgeI	ACCGGT	1 cut(s)	290
AgsI	TTSAA	2 cut(s)	676, 986
AjnI	CCWGG	1 cut(s)	1150
AleI	CACNNNNGTG	1 cut(s)	46
AloI	GAACNNNNNNTCC	2 cut(s)	489, 521
Alw21I	GWGCWC	3 cut(s)	390, 919, 974
Alw44I	GTGCAC	1 cut(s)	915
AlwI	GGATC	3 cut(s)	725, 823, 1162
AlwNI	CAGNNNCTG	1 cut(s)	842
AoxI	GGCC	4 cut(s)	275, 300, 585, 641
ApaLI	GTGCAC	1 cut(s)	915
ApeKI	GCWGC	5 cut(s)	458, 707, 743, 956, 1256
ApoI	RAATTY	4 cut(s)	410, 650, 986, 1038
AsiGI	ACCGGT	1 cut(s)	290
Asp700I	GAANNNNTTC	2 cut(s)	908, 1050
Asp718I	GGTACC	1 cut(s)	1147
AspA2I	CCTAGG	1 cut(s)	1168
AspLEI	GCGC	2 cut(s)	286, 545
AspS9I	GGNCC	3 cut(s)	4, 288, 642
AsuC2I	CCSGG	2 cut(s)	645, 808
AvaII	GGWCC	2 cut(s)	4, 288
AvrII	CCTAGG	1 cut(s)	1168
BaeGI	GKGCMC	1 cut(s)	919
BalI	TGGCCA	1 cut(s)	302
BanI	GGYRCC	1 cut(s)	1147
Bbv12I	GWGCWC	3 cut(s)	390, 919, 974
BbvCI	CCTCAGC	1 cut(s)	525
BbvI	GCAGC	5 cut(s)	445, 719, 755, 968, 1243
BccI	CCATC	2 cut(s)	267, 398
BciT130I	CCWGG	1 cut(s)	1152
BclI	TGATCA	1 cut(s)	555
BcnI	CCSGG	2 cut(s)	645, 808
BfaI	CTAG	5 cut(s)	621, 656, 783, 800, 1169
BfmI	CTRYAG	1 cut(s)	819
BfuAI	ACCTGC	1 cut(s)	82
BisI	GCNGC	5 cut(s)	459, 708, 744, 957, 1257
BlnI	CCTAGG	1 cut(s)	1168
BlsI	GCNGC	5 cut(s)	460, 709, 745, 958, 1258
Bme1390I	CCNGG	3 cut(s)	645, 808, 1152
Bme18I	GGWCC	2 cut(s)	4, 288
BmgT120I	GGNCC	3 cut(s)	4, 288, 642
BmiI	GGNNCC	3 cut(s)	23, 144, 1149
BmrFI	CCNGG	3 cut(s)	645, 808, 1152
BmsI	GCATC	5 cut(s)	343, 421, 644, 755, 761
BplI	GAGNNNNNCTC	2 cut(s)	92, 124
Bpu10I	CCTNAGC	1 cut(s)	525
BpuMI	CCSGG	2 cut(s)	645, 808
BsaJI	CCNNGG	2 cut(s)	807, 1168
BsaWI	WCCGGW	1 cut(s)	290
Bsc4I	CCNNNNNNNGG	2 cut(s)	594, 1113
Bse118I	RCCGGY	2 cut(s)	290, 520
Bse3DI	GCAATG	2 cut(s)	33, 450
BseBI	CCWGG	1 cut(s)	1152
BseDI	CCNNGG	2 cut(s)	807, 1168
BseGI	GGATG	7 cut(s)	124, 168, 175, 358, 409, 855, 1201
BseLI	CCNNNNNNNGG	2 cut(s)	594, 1113
BseMI	GCAATG	2 cut(s)	33, 450
BseMII	CTCAG	6 cut(s)	516, 879, 934, 969, 1083, 1293
BseRI	GAGGAG	2 cut(s)	190, 312
BseSI	GKGCMC	1 cut(s)	919
BseXI	GCAGC	5 cut(s)	445, 719, 755, 968, 1243
BseYI	CCCAGC	2 cut(s)	146, 659
BsgI	GTGCAG	1 cut(s)	249
Bsh1236I	CGCG	2 cut(s)	243, 286
BshFI	GGCC	4 cut(s)	277, 302, 587, 643
BshNI	GGYRCC	1 cut(s)	1147
BshTI	ACCGGT	1 cut(s)	290
BsiHKAI	GWGCWC	3 cut(s)	390, 919, 974
BsiSI	CCGG	4 cut(s)	291, 521, 645, 807
BslI	CCNNNNNNNGG	2 cut(s)	594, 1113
BsnI	GGCC	4 cut(s)	277, 302, 587, 643
Bsp1286I	GDGCHC	3 cut(s)	390, 919, 974
Bsp1407I	TGTACA	1 cut(s)	961
Bsp143I	GATC	4 cut(s)	555, 730, 828, 1154
Bsp68I	TCGCGA	1 cut(s)	243
BspACI	CCGC	1 cut(s)	286
BspANI	GGCC	4 cut(s)	277, 302, 587, 643
BspCNI	CTCAG	6 cut(s)	517, 878, 933, 970, 1084, 1294
BspFNI	CGCG	2 cut(s)	243, 286
BspLI	GGNNCC	3 cut(s)	23, 144, 1149
BspMI	ACCTGC	1 cut(s)	82
BspPI	GGATC	3 cut(s)	725, 823, 1162
BspT107I	GGYRCC	1 cut(s)	1147
BsrDI	GCAATG	2 cut(s)	33, 450
BsrFI	RCCGGY	2 cut(s)	290, 520
BsrGI	TGTACA	1 cut(s)	961
BssAI	RCCGGY	2 cut(s)	290, 520
BssECI	CCNNGG	2 cut(s)	807, 1168
BssMI	GATC	4 cut(s)	555, 730, 828, 1154
BssT1I	CCWWGG	1 cut(s)	1168
Bst2UI	CCWGG	1 cut(s)	1152
Bst4CI	ACNGT	3 cut(s)	502, 723, 1214
Bst6I	CTCTTC	1 cut(s)	930
BstAPI	GCANNNNNTGC	3 cut(s)	440, 842, 1265
BstAUI	TGTACA	1 cut(s)	961
BstC8I	GCNNGC	5 cut(s)	135, 245, 522, 767, 954
BstDEI	CTNAG	9 cut(s)	338, 525, 865, 920, 978, 1092, 1138, 1269, 1302
BstF5I	GGATG	7 cut(s)	124, 168, 175, 358, 409, 855, 1201
BstFNI	CGCG	2 cut(s)	243, 286
BstHHI	GCGC	2 cut(s)	286, 545
BstKTI	GATC	4 cut(s)	558, 733, 831, 1157
BstMBI	GATC	4 cut(s)	555, 730, 828, 1154
BstNI	CCWGG	1 cut(s)	1152
BstNSI	RCATGY	2 cut(s)	440, 1268
BstSCI	CCNGG	3 cut(s)	643, 806, 1150
BstSFI	CTRYAG	1 cut(s)	819
BstSLI	GKGCMC	1 cut(s)	919
BstUI	CGCG	2 cut(s)	243, 286
BstV1I	GCAGC	5 cut(s)	445, 719, 755, 968, 1243
BstXI	CCANNNNNNTGG	1 cut(s)	422
BsuRI	GGCC	4 cut(s)	277, 302, 587, 643
BtsCI	GGATG	7 cut(s)	124, 168, 175, 358, 409, 855, 1201
BtsIMutI	CAGTG	3 cut(s)	110, 919, 1089
BtuMI	TCGCGA	1 cut(s)	243
BveI	ACCTGC	1 cut(s)	82
Cac8I	GCNNGC	5 cut(s)	135, 245, 522, 767, 954
CaiI	CAGNNNCTG	1 cut(s)	842
CfoI	GCGC	2 cut(s)	286, 545
Cfr10I	RCCGGY	2 cut(s)	290, 520
Cfr13I	GGNCC	3 cut(s)	4, 288, 642
CpoI	CGGWCCG	1 cut(s)	288
Csp6I	GTAC	4 cut(s)	467, 882, 962, 1148
CspAI	ACCGGT	1 cut(s)	290
CspI	CGGWCCG	1 cut(s)	288
CviAII	CATG	8 cut(s)	188, 416, 428, 437, 553, 704, 1111, 1265
CviQI	GTAC	4 cut(s)	467, 882, 962, 1148
DdeI	CTNAG	9 cut(s)	338, 525, 865, 920, 978, 1092, 1138, 1269, 1302
DpnI	GATC	4 cut(s)	557, 732, 830, 1156
DpnII	GATC	4 cut(s)	555, 730, 828, 1154
DraIII	CACNNNGTG	1 cut(s)	607
EaeI	YGGCCR	1 cut(s)	300
Eam1104I	CTCTTC	1 cut(s)	930
EarI	CTCTTC	1 cut(s)	930
Eco130I	CCWWGG	1 cut(s)	1168
Eco32I	GATATC	2 cut(s)	617, 1240
Eco47I	GGWCC	2 cut(s)	4, 288
Eco57I	CTGAAG	2 cut(s)	134, 895
EcoRII	CCWGG	1 cut(s)	1150
EcoRV	GATATC	2 cut(s)	617, 1240
EcoT14I	CCWWGG	1 cut(s)	1168
ErhI	CCWWGG	1 cut(s)	1168
FaeI	CATG	8 cut(s)	191, 419, 431, 440, 556, 707, 1114, 1268
FalI	AAGNNNNNCTT	4 cut(s)	555, 587, 893, 925
FatI	CATG	8 cut(s)	187, 415, 427, 436, 552, 703, 1110, 1264
FauNDI	CATATG	1 cut(s)	208
FbaI	TGATCA	1 cut(s)	555
Fnu4HI	GCNGC	5 cut(s)	459, 708, 744, 957, 1257
FokI	GGATG	7 cut(s)	131, 175, 182, 365, 416, 842, 1188
Fsp4HI	GCNGC	5 cut(s)	459, 708, 744, 957, 1257
FspBI	CTAG	5 cut(s)	621, 656, 783, 800, 1169
GlaI	GCGC	2 cut(s)	285, 544
GluI	GCNGC	5 cut(s)	459, 708, 744, 957, 1257
GsaI	CCCAGC	2 cut(s)	150, 663
HaeIII	GGCC	4 cut(s)	277, 302, 587, 643
HapII	CCGG	4 cut(s)	291, 521, 645, 807
HhaI	GCGC	2 cut(s)	286, 545
Hin1II	CATG	8 cut(s)	191, 419, 431, 440, 556, 707, 1114, 1268
Hin6I	GCGC	2 cut(s)	284, 543
HinP1I	GCGC	2 cut(s)	284, 543
HindIII	AAGCTT	1 cut(s)	907
HinfI	GANTC	5 cut(s)	103, 598, 803, 863, 1295
HpaII	CCGG	4 cut(s)	291, 521, 645, 807
Hpy166II	GTNNAC	2 cut(s)	917, 1070
Hpy188I	TCNGA	1 cut(s)	1294
Hpy188III	TCNNGA	4 cut(s)	80, 242, 783, 800
Hpy8I	GTNNAC	2 cut(s)	917, 1070
Hpy99I	CGWCG	1 cut(s)	243
HpyAV	CCTTC	9 cut(s)	17, 109, 160, 533, 557, 722, 992, 1022, 1117
HpyCH4III	ACNGT	3 cut(s)	502, 723, 1214
HpyCH4IV	ACGT	2 cut(s)	532, 852
HpyF3I	CTNAG	9 cut(s)	338, 525, 865, 920, 978, 1092, 1138, 1269, 1302
HpySE526I	ACGT	2 cut(s)	532, 852
Hsp92II	CATG	8 cut(s)	191, 419, 431, 440, 556, 707, 1114, 1268
HspAI	GCGC	2 cut(s)	284, 543
KpnI	GGTACC	1 cut(s)	1151
KroI	GCCGGC	1 cut(s)	520
KroNI	GCCGGC	1 cut(s)	522
Ksp22I	TGATCA	1 cut(s)	555
Kzo9I	GATC	4 cut(s)	555, 730, 828, 1154
LmnI	GCTCC	4 cut(s)	385, 669, 1105, 1193
Lsp1109I	GCAGC	5 cut(s)	445, 719, 755, 968, 1243
LweI	GCATC	5 cut(s)	343, 421, 644, 755, 761
MaeI	CTAG	5 cut(s)	621, 656, 783, 800, 1169
MaeII	ACGT	2 cut(s)	532, 852
MalI	GATC	4 cut(s)	557, 732, 830, 1156
MboI	GATC	4 cut(s)	555, 730, 828, 1154
MboII	GAAGA	7 cut(s)	224, 494, 947, 1126, 1156, 1198, 1247
MfeI	CAATTG	1 cut(s)	1248
MhlI	GDGCHC	3 cut(s)	390, 919, 974
MlsI	TGGCCA	1 cut(s)	302
MluCI	AATT	9 cut(s)	157, 371, 410, 488, 650, 735, 986, 1038, 1248
MluNI	TGGCCA	1 cut(s)	302
MlyI	GAGTC	1 cut(s)	857
MmeI	TCCRAC	1 cut(s)	1057
Mox20I	TGGCCA	1 cut(s)	302
MroNI	GCCGGC	1 cut(s)	520
MroXI	GAANNNNTTC	2 cut(s)	908, 1050
MscI	TGGCCA	1 cut(s)	302
MseI	TTAA	1 cut(s)	849
MslI	CAYNNNNRTG	2 cut(s)	46, 420
Msp20I	TGGCCA	1 cut(s)	302
MspA1I	CMGCKG	1 cut(s)	959
MspI	CCGG	4 cut(s)	291, 521, 645, 807
MspR9I	CCNGG	3 cut(s)	645, 808, 1152
MunI	CAATTG	1 cut(s)	1248
MvaI	CCWGG	1 cut(s)	1152
MvnI	CGCG	2 cut(s)	243, 286
NaeI	GCCGGC	1 cut(s)	522
NciI	CCSGG	2 cut(s)	645, 808
NdeI	CATATG	1 cut(s)	208
NdeII	GATC	4 cut(s)	555, 730, 828, 1154
NgoMIV	GCCGGC	1 cut(s)	520
NlaIII	CATG	8 cut(s)	191, 419, 431, 440, 556, 707, 1114, 1268
NlaIV	GGNNCC	3 cut(s)	23, 144, 1149
NruI	TCGCGA	1 cut(s)	243
NspI	RCATGY	2 cut(s)	440, 1268
OliI	CACNNNNGTG	1 cut(s)	46
PaqCI	CACCTGC	1 cut(s)	82
PciI	ACATGT	1 cut(s)	436
PdiI	GCCGGC	1 cut(s)	522
PdmI	GAANNNNTTC	2 cut(s)	908, 1050
PfeI	GAWTC	4 cut(s)	103, 598, 803, 1295
PinAI	ACCGGT	1 cut(s)	290
PkrI	GCNGC	5 cut(s)	460, 709, 745, 958, 1258
PleI	GAGTC	1 cut(s)	857
PpsI	GAGTC	1 cut(s)	857
PscI	ACATGT	1 cut(s)	436
Psp6I	CCWGG	1 cut(s)	1150
PspFI	CCCAGC	2 cut(s)	146, 659
PspGI	CCWGG	1 cut(s)	1150
PspN4I	GGNNCC	3 cut(s)	23, 144, 1149
PspPI	GGNCC	3 cut(s)	4, 288, 642
PstNI	CAGNNNCTG	1 cut(s)	842
PvuII	CAGCTG	1 cut(s)	959
RruI	TCGCGA	1 cut(s)	243
RsaI	GTAC	4 cut(s)	468, 883, 963, 1149
RsaNI	GTAC	4 cut(s)	467, 882, 962, 1148
RseI	CAYNNNNRTG	2 cut(s)	46, 420
Rsr2I	CGGWCCG	1 cut(s)	288
RsrII	CGGWCCG	1 cut(s)	288
SaqAI	TTAA	1 cut(s)	849
SatI	GCNGC	5 cut(s)	459, 708, 744, 957, 1257
Sau3AI	GATC	4 cut(s)	555, 730, 828, 1154
Sau96I	GGNCC	3 cut(s)	4, 288, 642
SchI	GAGTC	1 cut(s)	857
ScrFI	CCNGG	3 cut(s)	645, 808, 1152
SduI	GDGCHC	3 cut(s)	390, 919, 974
SfaNI	GCATC	5 cut(s)	343, 421, 644, 755, 761
SfcI	CTRYAG	1 cut(s)	819
SinI	GGWCC	2 cut(s)	4, 288
SmiMI	CAYNNNNRTG	2 cut(s)	46, 420
Sse9I	AATT	9 cut(s)	157, 371, 410, 488, 650, 735, 986, 1038, 1248
SsiI	CCGC	1 cut(s)	286
SspMI	CTAG	5 cut(s)	621, 656, 783, 800, 1169
StyD4I	CCNGG	3 cut(s)	643, 806, 1150
StyI	CCWWGG	1 cut(s)	1168
TaaI	ACNGT	3 cut(s)	502, 723, 1214
TaiI	ACGT	2 cut(s)	535, 855
TaqI	TCGA	2 cut(s)	480, 1054
TasI	AATT	9 cut(s)	157, 371, 410, 488, 650, 735, 986, 1038, 1248
TatI	WGTACW	1 cut(s)	961
TfiI	GAWTC	4 cut(s)	103, 598, 803, 1295
Tru1I	TTAA	1 cut(s)	849
Tru9I	TTAA	1 cut(s)	849
TscAI	CASTG	3 cut(s)	117, 919, 1096
TseI	GCWGC	5 cut(s)	458, 707, 743, 956, 1256
TspDTI	ATGAA	9 cut(s)	179, 204, 225, 501, 1059, 1098, 1099, 1216, 1248
TspRI	CASTG	3 cut(s)	117, 919, 1096
VneI	GTGCAC	1 cut(s)	915
VpaK11BI	GGWCC	2 cut(s)	4, 288
XapI	RAATTY	4 cut(s)	410, 650, 986, 1038
XbaI	TCTAGA	2 cut(s)	782, 799
XceI	RCATGY	2 cut(s)	440, 1268
XmaJI	CCTAGG	1 cut(s)	1168
XmnI	GAANNNNTTC	2 cut(s)	908, 1050
XspI	CTAG	5 cut(s)	621, 656, 783, 800, 1169

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.