MD13G1088200.v1.1

MLP-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0013005
Physical Location & Seq
Forward (+)
6177671 .. 6178671
1001 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1088200.v1.1.491

Sequence Viewer

Length: 462 bp
ATGGCTTCATGTGATGGAAAGCTTGAAGTAGAAGTTGAGGTGAAGTCTCCTGCACAAAAGTTTTGGGAAGCCCTTAGGGACTCCACCACTGTCTTCCCCAAGGCATTTCCTCATGACTACAAAAGCATCGATGTCCTCGAAGGTGATGGCAAAGCTGTTGGATCCGTTCGGCTTATTACATATGCTGAAGGTTCTGCACTTGTGAAAGTATCAAAGGAGACGATCGATGCAGTGGATGAAGTCGGAAAGGCGGTTGCTTATAAGGTGATCGACGGAGATCTGCTCAAGTACTACAAGAGCTTCAAGTGCACCCTAACCGTGACTCCCAAGGGAGACGGGAGCTTGGTGAAATGGTCGTCTGTGTATGAGAAAGCACACGAACAGGTTCCCGAACCAAGCATCATCAAGGATTTTGCTGCCAAGAACTTCCTGGAGCTCGATGCCTATGTTCTGGTTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bet_v_1

Protein Analysis

154

Amino Acids

16.83

Weight (kDa)

5.26

Isoelectric Point (pI)

22.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 5 - 151 3e-40 Pathogenesis-related protein Bet v 1 family
Polyketide_cyc2 PF10604 7 - 140 2.9e-09 Polyketide cyclase / dehydrase and lipid transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013005)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G24020 AT1G24020
fragaria_vesca FvH4_4g34890
malus_domestica MD13G1088200.v1.1 MD16G1088600.v1.1
prunus_persica Prupe.1G258100_v2.0.a1
pyrus_communis pycom13g07600 pycom16g07580
rosa_chinensis RchiOBHm_Chr4g0444321
rosa_laevigata RLG00000005841
rosa_multiflora Rmu_sc0002841.1_g000013
rosa_roxburghii Rroxscaffold_5G00384870
rosa_rugosa Rorug04G0351500
rosa_samantha Rh4AG412200 Rh4BG423700 Rh4CG437900 Rh4DG419000
rosa_wichuraiana Rw4G035410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 261
AciI CCGC 1 cut(s) 251
AclWI GGATC 2 cut(s) 156, 169
AcuI CTGAAG 1 cut(s) 207
AfaI GTAC 1 cut(s) 290
AgsI TTSAA 2 cut(s) 26, 304
AjnI CCWGG 1 cut(s) 429
AluBI AGCT 5 cut(s) 22, 155, 300, 342, 436
AluI AGCT 5 cut(s) 22, 155, 300, 342, 436
Alw21I GWGCWC 2 cut(s) 311, 438
Alw26I GTCTC 3 cut(s) 51, 212, 327
Alw44I GTGCAC 1 cut(s) 307
AlwI GGATC 2 cut(s) 156, 169
ApaLI GTGCAC 1 cut(s) 307
ApeKI GCWGC 1 cut(s) 416
Asp700I GAANNNNTTC 1 cut(s) 384
AsuHPI GGTGA 4 cut(s) 52, 155, 277, 358
AxyI CCTNAGG 1 cut(s) 74
BaeGI GKGCMC 1 cut(s) 311
BamHI GGATCC 1 cut(s) 161
BanII GRGCYC 1 cut(s) 438
BbsI GAAGAC 1 cut(s) 85
Bbv12I GWGCWC 2 cut(s) 311, 438
BbvI GCAGC 1 cut(s) 403
BccI CCATC 2 cut(s) 8, 140
BciT130I CCWGG 1 cut(s) 431
BcoDI GTCTC 3 cut(s) 51, 212, 327
BglII AGATCT 1 cut(s) 277
BisI GCNGC 1 cut(s) 417
BlsI GCNGC 1 cut(s) 418
BmcAI AGTACT 1 cut(s) 290
Bme1390I CCNGG 1 cut(s) 431
BmiI GGNNCC 2 cut(s) 163, 387
BmrFI CCNGG 1 cut(s) 431
BmsI GCATC 4 cut(s) 135, 217, 408, 430
BpiI GAAGAC 1 cut(s) 85
BplI GAGNNNNNCTC 2 cut(s) 267, 299
BpmI CTGGAG 1 cut(s) 452
BpuEI CTTGAG 1 cut(s) 269
Bsa29I ATCGAT 2 cut(s) 129, 225
BsaJI CCNNGG 2 cut(s) 99, 327
BsaXI ACNNNNNCTCC 2 cut(s) 307, 337
Bse21I CCTNAGG 1 cut(s) 74
BseBI CCWGG 1 cut(s) 431
BseCI ATCGAT 2 cut(s) 129, 225
BseDI CCNNGG 2 cut(s) 99, 327
BseGI GGATG 1 cut(s) 241
BseSI GKGCMC 1 cut(s) 311
BseXI GCAGC 1 cut(s) 403
BsgI GTGCAG 2 cut(s) 36, 180
Bsh1285I CGRYCG 1 cut(s) 225
BshVI ATCGAT 2 cut(s) 129, 225
BsiEI CGRYCG 1 cut(s) 225
BsiHKAI GWGCWC 2 cut(s) 311, 438
BslFI GGGAC 1 cut(s) 92
BsmAI GTCTC 3 cut(s) 51, 212, 327
BsmBI CGTCTC 2 cut(s) 212, 327
BsmFI GGGAC 1 cut(s) 92
Bsp1286I GDGCHC 2 cut(s) 311, 438
Bsp143I GATC 4 cut(s) 161, 222, 267, 277
BspACI CCGC 1 cut(s) 251
BspDI ATCGAT 2 cut(s) 129, 225
BspHI TCATGA 1 cut(s) 112
BspLI GGNNCC 2 cut(s) 163, 387
BspPI GGATC 2 cut(s) 156, 169
BssECI CCNNGG 2 cut(s) 99, 327
BssMI GATC 4 cut(s) 161, 222, 267, 277
BssT1I CCWWGG 2 cut(s) 99, 327
Bst2UI CCWGG 1 cut(s) 431
Bst4CI ACNGT 2 cut(s) 91, 319
BstDEI CTNAG 1 cut(s) 74
BstF5I GGATG 1 cut(s) 241
BstKTI GATC 4 cut(s) 164, 225, 270, 280
BstMAI GTCTC 3 cut(s) 51, 212, 327
BstMBI GATC 4 cut(s) 161, 222, 267, 277
BstMCI CGRYCG 1 cut(s) 225
BstMWI GCNNNNNNNGC 1 cut(s) 306
BstNI CCWGG 1 cut(s) 431
BstSCI CCNGG 1 cut(s) 429
BstSLI GKGCMC 1 cut(s) 311
BstV1I GCAGC 1 cut(s) 403
BstV2I GAAGAC 1 cut(s) 85
BstX2I RGATCY 2 cut(s) 161, 277
BstYI RGATCY 2 cut(s) 161, 277
Bsu15I ATCGAT 2 cut(s) 129, 225
Bsu36I CCTNAGG 1 cut(s) 74
BsuTUI ATCGAT 2 cut(s) 129, 225
BtsCI GGATG 1 cut(s) 241
BtsI GCAGTG 1 cut(s) 237
BtsIMutI CAGTG 2 cut(s) 87, 237
CciI TCATGA 1 cut(s) 112
ClaI ATCGAT 2 cut(s) 129, 225
Csp6I GTAC 1 cut(s) 289
CviAII CATG 2 cut(s) 9, 113
CviJI RGCY 8 cut(s) 5, 22, 71, 155, 172, 300, 342, 436
CviKI_1 RGCY 8 cut(s) 5, 22, 71, 155, 172, 300, 342, 436
CviQI GTAC 1 cut(s) 289
DdeI CTNAG 1 cut(s) 74
DpnI GATC 4 cut(s) 163, 224, 269, 279
DpnII GATC 4 cut(s) 161, 222, 267, 277
Ecl136II GAGCTC 1 cut(s) 436
Eco130I CCWWGG 2 cut(s) 99, 327
Eco24I GRGCYC 1 cut(s) 438
Eco53kI GAGCTC 1 cut(s) 436
Eco57I CTGAAG 1 cut(s) 207
Eco81I CCTNAGG 1 cut(s) 74
EcoICRI GAGCTC 1 cut(s) 436
EcoRII CCWGG 1 cut(s) 429
EcoT14I CCWWGG 2 cut(s) 99, 327
EcoT38I GRGCYC 1 cut(s) 438
ErhI CCWWGG 2 cut(s) 99, 327
Esp3I CGTCTC 2 cut(s) 212, 327
FaeI CATG 2 cut(s) 12, 116
FaiI YATR 7 cut(s) 10, 114, 181, 183, 261, 366, 447
FaqI GGGAC 1 cut(s) 92
FatI CATG 2 cut(s) 8, 112
FauNDI CATATG 1 cut(s) 181
Fnu4HI GCNGC 1 cut(s) 417
FokI GGATG 1 cut(s) 248
FriOI GRGCYC 1 cut(s) 438
Fsp4HI GCNGC 1 cut(s) 417
GluI GCNGC 1 cut(s) 417
GsuI CTGGAG 1 cut(s) 452
Hin1II CATG 2 cut(s) 12, 116
HindIII AAGCTT 1 cut(s) 20
HinfI GANTC 2 cut(s) 80, 322
HphI GGTGA 4 cut(s) 52, 155, 277, 358
Hpy166II GTNNAC 1 cut(s) 309
Hpy188I TCNGA 1 cut(s) 245
Hpy188III TCNNGA 2 cut(s) 113, 389
Hpy8I GTNNAC 1 cut(s) 309
Hpy99I CGWCG 1 cut(s) 275
HpyAV CCTTC 2 cut(s) 134, 182
HpyCH4III ACNGT 2 cut(s) 91, 319
HpyCH4V TGCA 4 cut(s) 53, 197, 230, 309
HpyF10VI GCNNNNNNNGC 1 cut(s) 306
HpyF3I CTNAG 1 cut(s) 74
Hsp92II CATG 2 cut(s) 12, 116
Kzo9I GATC 4 cut(s) 161, 222, 267, 277
LmnI GCTCC 2 cut(s) 339, 433
LpnPI CCDG 5 cut(s) 63, 368, 416, 437, 443
Lsp1109I GCAGC 1 cut(s) 403
LweI GCATC 4 cut(s) 135, 217, 408, 430
MaeIII GTNAC 1 cut(s) 319
MalI GATC 4 cut(s) 163, 224, 269, 279
MboI GATC 4 cut(s) 161, 222, 267, 277
MboII GAAGA 1 cut(s) 85
MflI RGATCY 2 cut(s) 161, 277
MhlI GDGCHC 2 cut(s) 311, 438
MluCI AATT 1 cut(s) 457
MlyI GAGTC 2 cut(s) 74, 316
MmeI TCCRAC 2 cut(s) 139, 223
MnlI CCTC 3 cut(s) 31, 120, 146
MroXI GAANNNNTTC 1 cut(s) 384
MseI TTAA 2 cut(s) 456, 460
MspR9I CCNGG 1 cut(s) 431
MvaI CCWGG 1 cut(s) 431
MwoI GCNNNNNNNGC 1 cut(s) 306
NdeI CATATG 1 cut(s) 181
NdeII GATC 4 cut(s) 161, 222, 267, 277
NlaIII CATG 2 cut(s) 12, 116
NlaIV GGNNCC 2 cut(s) 163, 387
NmuCI GTSAC 1 cut(s) 319
PacI TTAATTAA 1 cut(s) 460
PagI TCATGA 1 cut(s) 112
PcsI WCGNNNNNNNCGW 1 cut(s) 135
PdmI GAANNNNTTC 1 cut(s) 384
PfoI TCCNGGA 1 cut(s) 429
PkrI GCNGC 1 cut(s) 418
Ple19I CGATCG 1 cut(s) 225
PleI GAGTC 2 cut(s) 74, 316
PpsI GAGTC 2 cut(s) 74, 316
PsiI TTATAA 1 cut(s) 261
Psp124BI GAGCTC 1 cut(s) 438
Psp6I CCWGG 1 cut(s) 429
PspGI CCWGG 1 cut(s) 429
PspN4I GGNNCC 2 cut(s) 163, 387
PsuI RGATCY 2 cut(s) 161, 277
PvuI CGATCG 1 cut(s) 225
RsaI GTAC 1 cut(s) 290
RsaNI GTAC 1 cut(s) 289
SacI GAGCTC 1 cut(s) 438
SaqAI TTAA 2 cut(s) 456, 460
SatI GCNGC 1 cut(s) 417
Sau3AI GATC 4 cut(s) 161, 222, 267, 277
ScaI AGTACT 1 cut(s) 290
SchI GAGTC 2 cut(s) 74, 316
ScrFI CCNGG 1 cut(s) 431
SduI GDGCHC 2 cut(s) 311, 438
SfaNI GCATC 4 cut(s) 135, 217, 408, 430
SmlI CTYRAG 1 cut(s) 284
SmoI CTYRAG 1 cut(s) 284
Sse9I AATT 1 cut(s) 457
SsiI CCGC 1 cut(s) 251
SstI GAGCTC 1 cut(s) 438
StyD4I CCNGG 1 cut(s) 429
StyI CCWWGG 2 cut(s) 99, 327
TaaI ACNGT 2 cut(s) 91, 319
TaqI TCGA 5 cut(s) 129, 138, 225, 270, 438
TasI AATT 1 cut(s) 457
TatI WGTACW 1 cut(s) 288
Tru1I TTAA 2 cut(s) 456, 460
Tru9I TTAA 2 cut(s) 456, 460
TscAI CASTG 2 cut(s) 94, 237
TseFI GTSAC 1 cut(s) 319
TseI GCWGC 1 cut(s) 416
Tsp45I GTSAC 1 cut(s) 319
TspDTI ATGAA 1 cut(s) 252
TspGWI ACGGA 2 cut(s) 154, 288
TspRI CASTG 2 cut(s) 94, 237
VneI GTGCAC 1 cut(s) 307
XcmI CCANNNNNNNNNTGG 1 cut(s) 427
XmnI GAANNNNTTC 1 cut(s) 384
ZrmI AGTACT 1 cut(s) 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.