Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0012265

Physical Location & Seq

Genomic Coordinates Reverse (-)

8347021 .. 8352470

Gene Structure 5450 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1115200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2103 bp

ATGGCGGACCTACCAATCCTCCAATTCGAGGACACAATCATGAAAGCAGTAGAAGAAAACCCCGTCGTAGTGGTGATCGGAGAGACAGGTTCGGGAAAGAGCACGCAGCTCTCTCAGATACTTCACAGAAGGGGCTACACCAAGTCCGGAGTCGTCGGCGTCACTCAGCCCCGCCGAGTCGCCGCCGTCTCCGTCGCCAGACGAGTTTCGCAGGAGCTCGGCGTTCGGCTTGGGGACGAAGTTGGATACGCCATCAGATTTGAAGATAGAACTTCGGAAAGGACGCGGATTAAGTACCTCACCGATGGAGTTCTTCTTCGTGAAAGCCTCTCCAATCCAGAGCTTGATCAGTACTCTGTAATCATACTAGATGAAGCTCACGAAAGGAGTCTAAACACGGACATACTGCTGGGACTGATGAAACGCTTGGTGAAAAGGCGTGCCTCGAATTTCAAGGTTCTAATCACTTCAGCAACTCTTGATGGCGAAAAAGTATCACAATTCTTTTCAAATTGCCCTGTAGTGAAAGTCCCCGGCAAGTTATTCCCGGTCGAAATAGCCTACAGCCGTGAGCGCCCTGCCAGCTATCTTGAGTCATCTCTAGAAACAGCTCTCAATATACATATTAAGCAAGGCGAAGGCGATGTCTTGATTTTCATGACTGGACAGGATGATATAGACAAGTTGGTGTTGAAGTTAGAAGAGAGAGTTCGAAGCCTGGAAGAAGGGTCTTGCATGGATGCCATAATCCTTCCTCTTCATGGTTCTTTGCAGCCCGAAATGCAGGTACGTGTTTTTGGTCCTCGGCCTCCAAATTGTAGGAGATTTATTGTTGCAACAAATATTGCTGAAACTTCTTTGACTGTAGATGGTGTTGTGTATGTTATTGATTCTGGTTACGTGAAGCAACGACAGTACAACCCATCAACTGGCATGTATTCCCTCGATGTTGTTCAAATTAGCAAGGTACAAGCAAATCAACGTGCAGGACGAGCAGGAAGAACACGTCCTGGGAAGTGCTATCGTTTATACCCTTCGACGGTTTACGAGGATGAGTTCCTTGATGCAACAATTCCTGAAATACAACGCTCTTCGCTTGCTGGAAGTGTTCTCTATTTGAAATCTTTGGACCTCCCTGATATTGACATTCTAAAATTTGATTTTCTTGATTCTCCTTCCCCTGAGTCTTTACAAGATGCTTTGAAGCAGTTGTATCTTATTGATGCTATTGATGAAAATGGATTGATCACAAGAGTTGGAAAAACAATGGCTGAGCTTCCGCTAGAACCTTCACTGTCAAAGACCTTAATGGAGGCAAATGAGTATGGCTGCTTATCGCAGGCTTTGACTGTTGCTGCTATGTTATCTGCAGAAACCACATTGCTTCCTGGTCGAAGCAAAAGTACGGAGAAGAAGAGGAAACATGATTATTTGGACCTTCCTGATGGCTCTGGTTGGGGTGATCATATCCAGTTGCTACAGATCTATGAGTGCTGGCATCAAACTGACTATGACATTAATTGGTGCAAGGATTATCAGTTGCAGGTGAGAGGGATGAAGTTTGTCAAGGATGTAAGGAAACAGTTATCTCAAATCATGCAGAAAATAGCAAAAGGATCATTAGATGTACAGACAGCCAGAAGACGGAATGAAAGCCAACAAGATTATCACAATTTGAGGAAGGCTTTGTGCGTGGGATATGCAAATCAACTTGCTGAGAGAATGATGCATCACAATGGTTATCGAACTTTAGGTTTTAAGCCCCAAGTAGTACAGGTGCATCCATCCTCTGTGCTAAAACCAGATGATGAAGGGAAATTGCCTGACTATGTTGTGTACCATGAACTCATTGCAACAACCCGCCCATTCCTGCGCACTGTATGTGCCGTAAATGTTGCTTGGGTCAGACCAATTTTGGACAAAGTGAATAATCTAAACATCAAGAAGCTGAGTGGCGGGATTGGTCGTATTGAAGAGCAACCGGAGGGAAATCTTTCCGATTTGCCAAAGAAAGAGGATGTTGTTGCGCCGGTCCTTGACGATCGGGAAAGTAGAGTACAGGCGGCTAGGGAACGATTTCTTGCTCGTAAAGGAAAGAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010467 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

AAA_22 DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

701

Amino Acids

78.75

Weight (kDa)

8.17

Isoelectric Point (pI)

46.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	13 - 167	2.7e-08	DEAD/DEAH box helicase
AAA_22	PF13401	21 - 136	1.8e-07	AAA domain
Helicase_C	PF00271	207 - 335	1.9e-10	Helicase conserved C-terminal domain
WHD_HA2	PF04408	400 - 428	3.5e-08	Helicase associated domain (HA2), winged-helix
HA2_C	PF21010	429 - 491	3.2e-08	Helicase associated domain (HA2), ratchet-like
OB_NTP_bind	PF07717	559 - 638	2.6e-19	Oligonucleotide/oligosaccharide-binding (OB)-fold

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0012265)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G27900 AT1G27900 AT1G27900
fragaria_vesca	FvH4_4g24140 FvH4_4g24140
malus_domestica	MD13G1115200.v1.1
prunus_persica	Prupe.1G232100_v2.0.a1 Prupe.1G232100_v2.0.a1
pyrus_communis	pycom13g09970
rosa_chinensis	RchiOBHm_Chr4g0430911
rosa_laevigata	RLG00000006939
rosa_multiflora	Rmu_sc0006263.1_g000011
rosa_roxburghii	Rroxscaffold_5G00372600
rosa_rugosa	Rorug04G0247300
rosa_samantha	Rh4AG302800 Rh4AG303700 Rh4BG310900 Rh4CG327300
rosa_wichuraiana	Rw4G026320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1534
Acc16I	TGCGCA	1 cut(s)	1874
Acc36I	ACCTGC	2 cut(s)	775, 1534
AccB7I	CCANNNNNTGG	1 cut(s)	929
AccII	CGCG	1 cut(s)	286
AccIII	TCCGGA	1 cut(s)	146
AciI	CCGC	8 cut(s)	5, 172, 183, 286, 1280, 1861, 1956, 2063
AclWI	GGATC	1 cut(s)	1624
AcsI	RAATTY	2 cut(s)	448, 1154
AcuI	CTGAAG	1 cut(s)	453
AcyI	GRCGYC	1 cut(s)	159
AfiI	CCNNNNNNNGG	5 cut(s)	28, 761, 929, 1039, 1644
AflIII	ACRYGT	2 cut(s)	790, 1004
AgsI	TTSAA	8 cut(s)	263, 454, 510, 694, 956, 1120, 1204, 1973
AjiI	CACGTC	1 cut(s)	1007
AjnI	CCWGG	3 cut(s)	717, 1009, 1387
AluBI	AGCT	8 cut(s)	109, 217, 343, 377, 585, 611, 1276, 1948
AluI	AGCT	8 cut(s)	109, 217, 343, 377, 585, 611, 1276, 1948
Alw21I	GWGCWC	2 cut(s)	104, 219
Alw26I	GTCTC	2 cut(s)	77, 193
AlwI	GGATC	1 cut(s)	1624
Aor13HI	TCCGGA	1 cut(s)	146
AoxI	GGCC	1 cut(s)	806
ApeKI	GCWGC	4 cut(s)	106, 772, 1329, 1355
ApoI	RAATTY	2 cut(s)	448, 1154
ArsI	GACNNNNNNTTYG	2 cut(s)	630, 662
AseI	ATTAAT	1 cut(s)	1518
Asp700I	GAANNNNTTC	2 cut(s)	1993, 2076
AspLEI	GCGC	3 cut(s)	576, 1875, 2029
AspS9I	GGNCC	5 cut(s)	7, 800, 1129, 1435, 2032
AsuC2I	CCSGG	2 cut(s)	534, 548
AsuHPI	GGTGA	5 cut(s)	85, 292, 442, 1472, 1558
AsuII	TTCGAA	1 cut(s)	712
AvaII	GGWCC	5 cut(s)	7, 800, 1129, 1435, 2032
BanII	GRGCYC	1 cut(s)	219
BarI	GAAGNNNNNNTAC	2 cut(s)	1387, 1419
BbsI	GAAGAC	1 cut(s)	1648
Bbv12I	GWGCWC	2 cut(s)	104, 219
BbvI	GCAGC	4 cut(s)	118, 784, 1316, 1342
BccI	CCATC	7 cut(s)	260, 299, 476, 863, 931, 1439, 1792
BceAI	ACGGC	3 cut(s)	170, 552, 1871
BciT130I	CCWGG	3 cut(s)	719, 1011, 1389
BciVI	GTATCC	1 cut(s)	239
BclI	TGATCA	3 cut(s)	346, 1245, 1462
BcnI	CCSGG	2 cut(s)	534, 548
BcoDI	GTCTC	2 cut(s)	77, 193
BfaI	CTAG	4 cut(s)	368, 602, 1283, 2067
BfmI	CTRYAG	5 cut(s)	519, 562, 864, 1368, 1478
BfoI	RGCGCY	1 cut(s)	577
BfuAI	ACCTGC	2 cut(s)	775, 1534
BfuI	GTATCC	1 cut(s)	239
BglII	AGATCT	1 cut(s)	1482
BisI	GCNGC	6 cut(s)	107, 183, 773, 1330, 1356, 2064
BlpI	GCTNAGC	1 cut(s)	1272
BlsI	GCNGC	6 cut(s)	108, 184, 774, 1331, 1357, 2065
BmcAI	AGTACT	1 cut(s)	353
Bme1390I	CCNGG	5 cut(s)	534, 548, 719, 1011, 1389
Bme18I	GGWCC	5 cut(s)	7, 800, 1129, 1435, 2032
BmgBI	CACGTC	1 cut(s)	1007
BmgT120I	GGNCC	5 cut(s)	7, 800, 1129, 1435, 2032
BmrFI	CCNGG	5 cut(s)	534, 548, 719, 1011, 1389
BmsI	GCATC	8 cut(s)	730, 1054, 1186, 1213, 1507, 1716, 1738, 1789
BpiI	GAAGAC	1 cut(s)	1648
Bpu1102I	GCTNAGC	1 cut(s)	1272
Bpu14I	TTCGAA	1 cut(s)	712
BpuEI	CTTGAG	1 cut(s)	611
BpuMI	CCSGG	2 cut(s)	534, 548
BsaAI	YACGTR	2 cut(s)	791, 901
BsaHI	GRCGYC	1 cut(s)	159
BsaJI	CCNNGG	3 cut(s)	532, 803, 1010
BsaWI	WCCGGW	2 cut(s)	146, 1981
BsaXI	ACNNNNNCTCC	1 cut(s)	33
Bsc4I	CCNNNNNNNGG	5 cut(s)	28, 761, 929, 1039, 1644
Bse118I	RCCGGY	1 cut(s)	2029
Bse1I	ACTGG	3 cut(s)	667, 934, 1471
Bse3DI	GCAATG	2 cut(s)	1379, 1848
BseAI	TCCGGA	1 cut(s)	146
BseBI	CCWGG	3 cut(s)	719, 1011, 1389
BseDI	CCNNGG	3 cut(s)	532, 803, 1010
BseGI	GGATG	8 cut(s)	676, 745, 1057, 1560, 1576, 1780, 1784, 2023
BseLI	CCNNNNNNNGG	5 cut(s)	28, 761, 929, 1039, 1644
BseMI	GCAATG	2 cut(s)	1379, 1848
BseMII	CTCAG	6 cut(s)	128, 179, 1173, 1263, 1707, 1940
BseNI	ACTGG	3 cut(s)	667, 934, 1471
BseXI	GCAGC	4 cut(s)	118, 784, 1316, 1342
BseYI	CCCAGC	1 cut(s)	409
BsgI	GTGCAG	1 cut(s)	1005
Bsh1236I	CGCG	1 cut(s)	286
Bsh1285I	CGRYCG	2 cut(s)	552, 2044
BshFI	GGCC	1 cut(s)	808
BsiEI	CGRYCG	2 cut(s)	552, 2044
BsiHKAI	GWGCWC	2 cut(s)	104, 219
BsiSI	CCGG	5 cut(s)	147, 534, 548, 1982, 2030
BslFI	GGGAC	3 cut(s)	248, 426, 515
BslI	CCNNNNNNNGG	5 cut(s)	28, 761, 929, 1039, 1644
BsmAI	GTCTC	2 cut(s)	77, 193
BsmBI	CGTCTC	1 cut(s)	193
BsmFI	GGGAC	3 cut(s)	248, 426, 515
BsnI	GGCC	1 cut(s)	808
Bsp119I	TTCGAA	1 cut(s)	712
Bsp1286I	GDGCHC	2 cut(s)	104, 219
Bsp13I	TCCGGA	1 cut(s)	146
Bsp1407I	TGTACA	1 cut(s)	1627
Bsp143I	GATC	7 cut(s)	75, 346, 1245, 1462, 1482, 1616, 2041
Bsp1720I	GCTNAGC	1 cut(s)	1272
BspACI	CCGC	8 cut(s)	5, 172, 183, 286, 1280, 1861, 1956, 2063
BspANI	GGCC	1 cut(s)	808
BspCNI	CTCAG	6 cut(s)	127, 178, 1174, 1264, 1708, 1941
BspEI	TCCGGA	1 cut(s)	146
BspFNI	CGCG	1 cut(s)	286
BspHI	TCATGA	2 cut(s)	39, 657
BspMAI	CTGCAG	1 cut(s)	1372
BspMI	ACCTGC	2 cut(s)	775, 1534
BspPI	GGATC	1 cut(s)	1624
BspQI	GCTCTTC	2 cut(s)	1096, 1968
BspT104I	TTCGAA	1 cut(s)	712
BsrDI	GCAATG	2 cut(s)	1379, 1848
BsrFI	RCCGGY	1 cut(s)	2029
BsrGI	TGTACA	1 cut(s)	1627
BsrI	ACTGG	3 cut(s)	667, 934, 1471
BssAI	RCCGGY	1 cut(s)	2029
BssECI	CCNNGG	3 cut(s)	532, 803, 1010
BssMI	GATC	7 cut(s)	75, 346, 1245, 1462, 1482, 1616, 2041
BssNI	GRCGYC	1 cut(s)	159
Bst2UI	CCWGG	3 cut(s)	719, 1011, 1389
Bst4CI	ACNGT	7 cut(s)	865, 915, 1042, 1296, 1351, 1584, 1879
Bst6I	CTCTTC	5 cut(s)	696, 762, 1096, 1409, 1968
BstACI	GRCGYC	1 cut(s)	159
BstAUI	TGTACA	1 cut(s)	1627
BstBAI	YACGTR	2 cut(s)	791, 901
BstBI	TTCGAA	1 cut(s)	712
BstC8I	GCNNGC	6 cut(s)	104, 441, 583, 1098, 1341, 1496
BstDEI	CTNAG	6 cut(s)	114, 165, 1182, 1272, 1716, 1949
BstF5I	GGATG	8 cut(s)	676, 745, 1057, 1560, 1576, 1780, 1784, 2023
BstFNI	CGCG	1 cut(s)	286
BstH2I	RGCGCY	1 cut(s)	577
BstHHI	GCGC	3 cut(s)	576, 1875, 2029
BstKTI	GATC	7 cut(s)	78, 349, 1248, 1465, 1485, 1619, 2044
BstMAI	GTCTC	2 cut(s)	77, 193
BstMBI	GATC	7 cut(s)	75, 346, 1245, 1462, 1482, 1616, 2041
BstMCI	CGRYCG	2 cut(s)	552, 2044
BstMWI	GCNNNNNNNGC	4 cut(s)	573, 582, 781, 992
BstNI	CCWGG	3 cut(s)	719, 1011, 1389
BstNSI	RCATGY	1 cut(s)	937
BstSCI	CCNGG	5 cut(s)	532, 546, 717, 1009, 1387
BstSFI	CTRYAG	5 cut(s)	519, 562, 864, 1368, 1478
BstUI	CGCG	1 cut(s)	286
BstV1I	GCAGC	4 cut(s)	118, 784, 1316, 1342
BstV2I	GAAGAC	1 cut(s)	1648
BstX2I	RGATCY	1 cut(s)	1482
BstYI	RGATCY	1 cut(s)	1482
BsuI	GTATCC	1 cut(s)	239
BsuRI	GGCC	1 cut(s)	808
BtgZI	GCGATG	1 cut(s)	657
BtrI	CACGTC	1 cut(s)	1007
BtsCI	GGATG	8 cut(s)	676, 745, 1057, 1560, 1576, 1780, 1784, 2023
BtsIMutI	CAGTG	2 cut(s)	1292, 1875
BveI	ACCTGC	2 cut(s)	775, 1534
Cac8I	GCNNGC	6 cut(s)	104, 441, 583, 1098, 1341, 1496
CciI	TCATGA	2 cut(s)	39, 657
CfoI	GCGC	3 cut(s)	576, 1875, 2029
Cfr10I	RCCGGY	1 cut(s)	2029
Cfr13I	GGNCC	5 cut(s)	7, 800, 1129, 1435, 2032
CseI	GACGC	2 cut(s)	148, 292
CviAII	CATG	8 cut(s)	40, 658, 736, 761, 934, 1424, 1597, 1841
DdeI	CTNAG	6 cut(s)	114, 165, 1182, 1272, 1716, 1949
DpnI	GATC	7 cut(s)	77, 348, 1247, 1464, 1484, 1618, 2043
DpnII	GATC	7 cut(s)	75, 346, 1245, 1462, 1482, 1616, 2041
Eam1104I	CTCTTC	5 cut(s)	696, 762, 1096, 1409, 1968
EarI	CTCTTC	5 cut(s)	696, 762, 1096, 1409, 1968
EciI	GGCGGA	1 cut(s)	20
Ecl136II	GAGCTC	1 cut(s)	217
Eco24I	GRGCYC	1 cut(s)	219
Eco47I	GGWCC	5 cut(s)	7, 800, 1129, 1435, 2032
Eco53kI	GAGCTC	1 cut(s)	217
Eco57I	CTGAAG	1 cut(s)	453
EcoICRI	GAGCTC	1 cut(s)	217
EcoRII	CCWGG	3 cut(s)	717, 1009, 1387
EcoT22I	ATGCAT	1 cut(s)	1731
EcoT38I	GRGCYC	1 cut(s)	219
Esp3I	CGTCTC	1 cut(s)	193
FaeI	CATG	8 cut(s)	43, 661, 739, 764, 937, 1427, 1600, 1844
FaqI	GGGAC	3 cut(s)	248, 426, 515
FatI	CATG	8 cut(s)	39, 657, 735, 760, 933, 1423, 1596, 1840
FauI	CCCGC	3 cut(s)	179, 1868, 1949
FbaI	TGATCA	3 cut(s)	346, 1245, 1462
Fnu4HI	GCNGC	6 cut(s)	107, 183, 773, 1330, 1356, 2064
FokI	GGATG	8 cut(s)	683, 752, 1064, 1567, 1583, 1767, 1771, 2030
FriOI	GRGCYC	1 cut(s)	219
Fsp4HI	GCNGC	6 cut(s)	107, 183, 773, 1330, 1356, 2064
FspBI	CTAG	4 cut(s)	368, 602, 1283, 2067
FspI	TGCGCA	1 cut(s)	1874
GlaI	GCGC	3 cut(s)	575, 1874, 2028
GluI	GCNGC	6 cut(s)	107, 183, 773, 1330, 1356, 2064
GsaI	CCCAGC	1 cut(s)	413
HaeII	RGCGCY	1 cut(s)	577
HaeIII	GGCC	1 cut(s)	808
HapII	CCGG	5 cut(s)	147, 534, 548, 1982, 2030
HgaI	GACGC	2 cut(s)	148, 292
HhaI	GCGC	3 cut(s)	576, 1875, 2029
Hin1I	GRCGYC	1 cut(s)	159
Hin1II	CATG	8 cut(s)	43, 661, 739, 764, 937, 1427, 1600, 1844
Hin6I	GCGC	3 cut(s)	574, 1873, 2027
HinP1I	GCGC	3 cut(s)	574, 1873, 2027
HinfI	GANTC	7 cut(s)	150, 177, 388, 593, 890, 1169, 1184
HpaII	CCGG	5 cut(s)	147, 534, 548, 1982, 2030
HphI	GGTGA	5 cut(s)	85, 292, 442, 1472, 1558
Hpy166II	GTNNAC	2 cut(s)	1045, 1837
Hpy188I	TCNGA	6 cut(s)	80, 117, 257, 277, 1907, 1999
Hpy8I	GTNNAC	2 cut(s)	1045, 1837
Hpy99I	CGWCG	4 cut(s)	68, 158, 197, 1042
HpyCH4III	ACNGT	7 cut(s)	865, 915, 1042, 1296, 1351, 1584, 1879
HpyCH4IV	ACGT	4 cut(s)	790, 900, 982, 1006
HpyF10VI	GCNNNNNNNGC	4 cut(s)	573, 582, 781, 992
HpyF3I	CTNAG	6 cut(s)	114, 165, 1182, 1272, 1716, 1949
HpySE526I	ACGT	4 cut(s)	790, 900, 982, 1006
Hsp92I	GRCGYC	1 cut(s)	159
Hsp92II	CATG	8 cut(s)	43, 661, 739, 764, 937, 1427, 1600, 1844
HspAI	GCGC	3 cut(s)	574, 1873, 2027
Kpn2I	TCCGGA	1 cut(s)	146
Ksp22I	TGATCA	3 cut(s)	346, 1245, 1462
Kzo9I	GATC	7 cut(s)	75, 346, 1245, 1462, 1482, 1616, 2041
LguI	GCTCTTC	2 cut(s)	1096, 1968
LmnI	GCTCC	1 cut(s)	214
Lsp1109I	GCAGC	4 cut(s)	118, 784, 1316, 1342
LweI	GCATC	8 cut(s)	730, 1054, 1186, 1213, 1507, 1716, 1738, 1789
MaeI	CTAG	4 cut(s)	368, 602, 1283, 2067
MaeII	ACGT	4 cut(s)	790, 900, 982, 1006
MaeIII	GTNAC	2 cut(s)	160, 896
MalI	GATC	7 cut(s)	77, 348, 1247, 1464, 1484, 1618, 2043
MboI	GATC	7 cut(s)	75, 346, 1245, 1462, 1482, 1616, 2041
MflI	RGATCY	1 cut(s)	1482
MhlI	GDGCHC	2 cut(s)	104, 219
MlyI	GAGTC	5 cut(s)	159, 186, 397, 602, 1193
MmeI	TCCRAC	2 cut(s)	223, 1237
Mph1103I	ATGCAT	1 cut(s)	1731
MroI	TCCGGA	1 cut(s)	146
MroXI	GAANNNNTTC	2 cut(s)	1993, 2076
MseI	TTAA	5 cut(s)	291, 627, 1307, 1518, 1758
MslI	CAYNNNNRTG	2 cut(s)	38, 1734
MspI	CCGG	5 cut(s)	147, 534, 548, 1982, 2030
MspR9I	CCNGG	5 cut(s)	534, 548, 719, 1011, 1389
MvaI	CCWGG	3 cut(s)	719, 1011, 1389
MvnI	CGCG	1 cut(s)	286
MwoI	GCNNNNNNNGC	4 cut(s)	573, 582, 781, 992
NciI	CCSGG	2 cut(s)	534, 548
NdeII	GATC	7 cut(s)	75, 346, 1245, 1462, 1482, 1616, 2041
NlaIII	CATG	8 cut(s)	43, 661, 739, 764, 937, 1427, 1600, 1844
NmeAIII	GCCGAG	3 cut(s)	198, 200, 784
NmuCI	GTSAC	1 cut(s)	160
NsbI	TGCGCA	1 cut(s)	1874
NsiI	ATGCAT	1 cut(s)	1731
NspI	RCATGY	1 cut(s)	937
NspV	TTCGAA	1 cut(s)	712
PagI	TCATGA	2 cut(s)	39, 657
PaqCI	CACCTGC	1 cut(s)	1534
PciSI	GCTCTTC	2 cut(s)	1096, 1968
PcsI	WCGNNNNNNNCGW	1 cut(s)	988
PdmI	GAANNNNTTC	2 cut(s)	1993, 2076
PfeI	GAWTC	2 cut(s)	890, 1169
PflMI	CCANNNNNTGG	1 cut(s)	929
PkrI	GCNGC	6 cut(s)	108, 184, 774, 1331, 1357, 2065
Ple19I	CGATCG	1 cut(s)	2044
PleI	GAGTC	5 cut(s)	158, 185, 396, 601, 1192
PpsI	GAGTC	5 cut(s)	158, 185, 396, 601, 1192
Ppu21I	YACGTR	2 cut(s)	791, 901
PshBI	ATTAAT	1 cut(s)	1518
Psp124BI	GAGCTC	1 cut(s)	219
Psp6I	CCWGG	3 cut(s)	717, 1009, 1387
PspFI	CCCAGC	1 cut(s)	409
PspGI	CCWGG	3 cut(s)	717, 1009, 1387
PspPI	GGNCC	5 cut(s)	7, 800, 1129, 1435, 2032
PstI	CTGCAG	1 cut(s)	1372
PsuI	RGATCY	1 cut(s)	1482
PvuI	CGATCG	1 cut(s)	2044
RseI	CAYNNNNRTG	2 cut(s)	38, 1734
SacI	GAGCTC	1 cut(s)	219
SapI	GCTCTTC	2 cut(s)	1096, 1968
SaqAI	TTAA	5 cut(s)	291, 627, 1307, 1518, 1758
SatI	GCNGC	6 cut(s)	107, 183, 773, 1330, 1356, 2064
Sau3AI	GATC	7 cut(s)	75, 346, 1245, 1462, 1482, 1616, 2041
Sau96I	GGNCC	5 cut(s)	7, 800, 1129, 1435, 2032
ScaI	AGTACT	1 cut(s)	353
SchI	GAGTC	5 cut(s)	159, 186, 397, 602, 1193
ScrFI	CCNGG	5 cut(s)	534, 548, 719, 1011, 1389
SduI	GDGCHC	2 cut(s)	104, 219
SfaNI	GCATC	8 cut(s)	730, 1054, 1186, 1213, 1507, 1716, 1738, 1789
SfcI	CTRYAG	5 cut(s)	519, 562, 864, 1368, 1478
SfuI	TTCGAA	1 cut(s)	712
SinI	GGWCC	5 cut(s)	7, 800, 1129, 1435, 2032
SmiMI	CAYNNNNRTG	2 cut(s)	38, 1734
SmlI	CTYRAG	1 cut(s)	590
SmoI	CTYRAG	1 cut(s)	590
SsiI	CCGC	8 cut(s)	5, 172, 183, 286, 1280, 1861, 1956, 2063
SspI	AATATT	1 cut(s)	844
SspMI	CTAG	4 cut(s)	368, 602, 1283, 2067
SstI	GAGCTC	1 cut(s)	219
StyD4I	CCNGG	5 cut(s)	532, 546, 717, 1009, 1387
TaaI	ACNGT	7 cut(s)	865, 915, 1042, 1296, 1351, 1584, 1879
TaiI	ACGT	4 cut(s)	793, 903, 985, 1009
TaqI	TCGA	8 cut(s)	27, 446, 552, 712, 945, 1037, 1393, 1744
TatI	WGTACW	5 cut(s)	351, 915, 1627, 1771, 2056
TauI	GCSGC	2 cut(s)	185, 2066
TfiI	GAWTC	2 cut(s)	890, 1169
Tru1I	TTAA	5 cut(s)	291, 627, 1307, 1518, 1758
Tru9I	TTAA	5 cut(s)	291, 627, 1307, 1518, 1758
TscAI	CASTG	2 cut(s)	1299, 1882
TseFI	GTSAC	1 cut(s)	160
TseI	GCWGC	4 cut(s)	106, 772, 1329, 1355
Tsp45I	GTSAC	1 cut(s)	160
TspGWI	ACGGA	4 cut(s)	181, 413, 1421, 1660
TspRI	CASTG	2 cut(s)	1299, 1882
Van91I	CCANNNNNTGG	1 cut(s)	929
VpaK11BI	GGWCC	5 cut(s)	7, 800, 1129, 1435, 2032
VspI	ATTAAT	1 cut(s)	1518
XapI	RAATTY	2 cut(s)	448, 1154
XbaI	TCTAGA	1 cut(s)	601
XceI	RCATGY	1 cut(s)	937
XmnI	GAANNNNTTC	2 cut(s)	1993, 2076
XspI	CTAG	4 cut(s)	368, 602, 1283, 2067
ZrmI	AGTACT	1 cut(s)	353
Zsp2I	ATGCAT	1 cut(s)	1731

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.