Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0012265

Physical Location & Seq

Genomic Coordinates Forward (+)

54011132 .. 54016812

Gene Structure 5681 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00372600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2157 bp

ATGGCGGAGCTCCCAATCCTTCAGTTCGAAGAGAAAATCATCAAAACAGTAGAACGGAACCCCGTCGTTGTCATCATCGGAGAGACCGGCTCCGGAAAGAGCACGCAGCTCTCTCAAATCCTCCACAGAAGAGGCTACACCAACTCCGGCGTCGTCGGCGTCACCCAGCCTCGCCGTGTCGCCGCCGTCTCCGTCGCCAGACGAGTTTCGCAGGAGCTCAATGTTCGGCTCGGCGAGGAAGTCGGATACGCCATTAGATTCGAAGATAGAACTTCCGAAAGGACTCGGATCAAGTACTTGACCGATGGAGTTCTTCTTCGTGAGAGCCTATCCAATCCGGAGCTGGATCAGTACTCTGTAATTATACTTGACGAGGCTCACGAGAGAAGTCTCAACACGGACATATTGCTGGGACTGATGAAACGATTGGTGAAGAGGCGTGCTTCGAATTTGAAGGTTCTTATTACTTCGGCGACTCTTGATGGTGATAAAGTATCACAGTTCTTTTCGAATTGTCCGGTGCTGAATGTTCCGGGGAAGCTATACCCTGTGGAGATATTGTACAGCAATGAGCGTCCTAAGAGCTATCTTGAGTCGTCTTTGAAAACAGCTCTCGATATACATATTAAGGAACCTGAAGGAGATGTCTTGATATTCATGACTGGGCAGGATGACATAGAGAAGTTGGTCTCTAAGTTAGAAGATAGAGTACGTGTATTTAGTCCCCCACCTCCTAATTGTAGGAGATTTATTGTTGCCACAAACATTGCTGAAACTTCTTTGACTGTTGATGGTGTTGTGTATGTTATTGATTCTGGTTATGTGAAGCAACGGCAGTACAACCCCTCAACTGGCATGTACTCCCTCGAAGTTGTTCAAATTAGCAAGGTACAAGCTAATCAACGAGCAGGACGAGCTGGAAGGACACGTCCTGGGAAATGCTATCGGTTATATCCTTCCATTGCTTACCATGAGGAGCTGCTGGATGCAACAATTCCTGAAATTCAACGTTCTTCACTTGCTGGAAGTGTTCTTTATTTGAAATCCTTGGACCTCCCTGATATTGACATTCTCACATTCGATTTTCTTGATTCTCCTTCCTCTGAATCTTTACAAGATGCTCTGAAGCAGTTGTATCTTATTGATGCTATTGATGAAAATGGATTGATCACGAGAGTTGGGAAAACAATGGCTGAGCTTCCGCTAGAACCTTCACTGTCAAGAACCTTAATGGAGGCGAATGAGTGTGGTTGCTTGTCCCAGGCTTTGACAGTTGCTGCTATGTTATCTGCAGAAACTACATTGCTTCCTGGTCGAAGCAAGAGTACTGAGAAGAAAAGGAAACATGATCATTTGGACCTTCCTGATAGTTCTGGTTGGGGTGATCACATCCAGTTGCTACAGATCTATGAGTGCTGGCATCAAACTCACTATGACATTGATTGGTGCAAGGACCGTGGCTTGCAGGTAAGAGGGATGACGTTTGTCAAGGATGTAAGGAAACAGCTATGTCAAATAATGCAAAAAATAGCAAAGGGTTATCTAGAGGTTGTCTTCTTGCATTGTTCTCTGGTCCAAGTTGTTACTATTTACTTCCACGGTTCCACTGGAATATGTCATGTTGATGTTGTATTCCTTGCACTGACAAAACATTTTGTACCTGTAAACACAGGATCGTTAGATGTACAGACAAGCAGAAGAGGGAAACAAACCCAACAGGATTACAATAACTTGAGGAAGGCTTTGTGTGTGGGGTATGCAAATCAGCTTGCTGAGAGAATGGTATTTCACAATGGCTATCGAACTTTAGGTTTTAAGCCCCAAGTAGTACAGGTGCATCCATCCTCTCTGCTAAAACCAGATGATGAAGGGAAATTTCCAAATTATGTCGTGTACCAGGAACTTATTGCCACTTCTCGTCCATACTTGCGCACTGTATGTGCTGTAAATGTTGCATGGGTTACACCCATTTTGAACAAGGTTAATAAACTAAACATCAACAAGCTGAGTGGTGGGAGTGGTCATATTGAAGAGCATGCAGAGGGAAATATTTCTGATTTGCCAAAGAAAGATGCTGTTACAGCAGTTCCTGATGATCAAGAAAGTAAAATACAAGCAGCCAGAGAACGATTTCTTGCTCGTAAAGGAAAGAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010467 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

AAA_22 DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

718

Amino Acids

80.54

Weight (kDa)

8.5

Isoelectric Point (pI)

44.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	11 - 166	2.2e-08	DEAD/DEAH box helicase
AAA_22	PF13401	21 - 136	3.6e-07	AAA domain
Helicase_C	PF00271	234 - 309	2.9e-06	Helicase conserved C-terminal domain
WHD_HA2	PF04408	374 - 402	3.6e-08	Helicase associated domain (HA2), winged-helix
HA2_C	PF21010	403 - 466	6.1e-08	Helicase associated domain (HA2), ratchet-like
OB_NTP_bind	PF07717	578 - 658	2.3e-17	Oligonucleotide/oligosaccharide-binding (OB)-fold

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012265)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G27900 AT1G27900 AT1G27900
fragaria_vesca	FvH4_4g24140 FvH4_4g24140
malus_domestica	MD13G1115200.v1.1
prunus_persica	Prupe.1G232100_v2.0.a1 Prupe.1G232100_v2.0.a1
pyrus_communis	pycom13g09970
rosa_chinensis	RchiOBHm_Chr4g0430911
rosa_laevigata	RLG00000006939
rosa_multiflora	Rmu_sc0006263.1_g000011
rosa_roxburghii	Rroxscaffold_5G00372600
rosa_rugosa	Rorug04G0247300
rosa_samantha	Rh4AG302800 Rh4AG303700 Rh4BG310900 Rh4CG327300
rosa_wichuraiana	Rw4G026320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1931
Acc36I	ACCTGC	1 cut(s)	1456
AccIII	TCCGGA	2 cut(s)	92, 337
AciI	CCGC	3 cut(s)	5, 183, 1202
AclI	AACGTT	1 cut(s)	1009
AclWI	GGATC	3 cut(s)	296, 354, 1681
AcsI	RAATTY	3 cut(s)	448, 1002, 1874
AcuI	CTGAAG	3 cut(s)	5, 657, 1145
AcyI	GRCGYC	2 cut(s)	150, 159
AfiI	CCNNNNNNNGG	1 cut(s)	851
AflIII	ACRYGT	2 cut(s)	712, 926
AgsI	TTSAA	7 cut(s)	454, 604, 878, 1007, 1042, 1975, 2030
AjiI	CACGTC	1 cut(s)	929
AjnI	CCWGG	4 cut(s)	931, 1260, 1309, 1896
Alw21I	GWGCWC	3 cut(s)	12, 104, 219
Alw26I	GTCTC	4 cut(s)	77, 193, 395, 694
AlwI	GGATC	3 cut(s)	296, 354, 1681
AlwNI	CAGNNNCTG	2 cut(s)	1277, 2090
Aor13HI	TCCGGA	2 cut(s)	92, 337
ApeKI	GCWGC	4 cut(s)	106, 979, 1277, 2117
ApoI	RAATTY	3 cut(s)	448, 1002, 1874
Asp700I	GAANNNNTTC	3 cut(s)	873, 2050, 2130
AspLEI	GCGC	1 cut(s)	1932
AspS9I	GGNCC	4 cut(s)	1051, 1357, 1453, 1573
AsuC2I	CCSGG	1 cut(s)	534
AsuHPI	GGTGA	4 cut(s)	154, 442, 497, 1394
AsuII	TTCGAA	4 cut(s)	27, 261, 446, 509
AvaII	GGWCC	4 cut(s)	1051, 1357, 1453, 1573
BanII	GRGCYC	2 cut(s)	12, 219
BarI	GAAGNNNNNNTAC	4 cut(s)	693, 725, 1309, 1341
BauI	CACGAG	2 cut(s)	380, 1171
BbsI	GAAGAC	1 cut(s)	1546
Bbv12I	GWGCWC	3 cut(s)	12, 104, 219
BbvI	GCAGC	4 cut(s)	118, 966, 1264, 2129
BccI	CCATC	4 cut(s)	299, 476, 785, 1849
BceAI	ACGGC	3 cut(s)	159, 170, 848
BciT130I	CCWGG	4 cut(s)	933, 1262, 1311, 1898
BciVI	GTATCC	1 cut(s)	239
BclI	TGATCA	4 cut(s)	1167, 1348, 1384, 2095
BcnI	CCSGG	1 cut(s)	534
BcoDI	GTCTC	4 cut(s)	77, 193, 395, 694
BfaI	CTAG	2 cut(s)	1205, 1544
BfmI	CTRYAG	2 cut(s)	1290, 1400
BfuAI	ACCTGC	1 cut(s)	1456
BfuI	GTATCC	1 cut(s)	239
BglII	AGATCT	1 cut(s)	1404
BisI	GCNGC	5 cut(s)	107, 183, 980, 1278, 2118
BlpI	GCTNAGC	1 cut(s)	1194
BlsI	GCNGC	5 cut(s)	108, 184, 981, 1279, 2119
BmcAI	AGTACT	3 cut(s)	296, 353, 1327
Bme1390I	CCNGG	5 cut(s)	534, 933, 1262, 1311, 1898
Bme18I	GGWCC	4 cut(s)	1051, 1357, 1453, 1573
BmgBI	CACGTC	1 cut(s)	929
BmgT120I	GGNCC	4 cut(s)	1051, 1357, 1453, 1573
BmiI	GGNNCC	4 cut(s)	59, 91, 633, 1603
BmrFI	CCNGG	5 cut(s)	534, 933, 1262, 1311, 1898
BmrI	ACTGGG	1 cut(s)	672
BmsI	GCATC	6 cut(s)	976, 1108, 1135, 1429, 1846, 2062
BmuI	ACTGGG	1 cut(s)	672
BoxI	GACNNNNGTC	1 cut(s)	1484
BpiI	GAAGAC	1 cut(s)	1546
BplI	GAGNNNNNCTC	2 cut(s)	74, 106
Bpu1102I	GCTNAGC	1 cut(s)	1194
Bpu14I	TTCGAA	4 cut(s)	27, 261, 446, 509
BpuEI	CTTGAG	2 cut(s)	611, 1753
BpuMI	CCSGG	1 cut(s)	534
BsaAI	YACGTR	1 cut(s)	713
BsaHI	GRCGYC	2 cut(s)	150, 159
BsaI	GGTCTC	2 cut(s)	77, 694
BsaJI	CCNNGG	6 cut(s)	533, 932, 1047, 1260, 1456, 1597
BsaWI	WCCGGW	3 cut(s)	92, 337, 517
BsaXI	ACNNNNNCTCC	4 cut(s)	128, 158, 545, 575
Bsc4I	CCNNNNNNNGG	1 cut(s)	851
Bse118I	RCCGGY	1 cut(s)	86
Bse1I	ACTGG	4 cut(s)	667, 856, 1393, 1612
Bse3DI	GCAATG	4 cut(s)	574, 765, 960, 1301
BseAI	TCCGGA	2 cut(s)	92, 337
BseBI	CCWGG	4 cut(s)	933, 1262, 1311, 1898
BseDI	CCNNGG	6 cut(s)	533, 932, 1047, 1260, 1456, 1597
BseGI	GGATG	7 cut(s)	676, 991, 1389, 1482, 1498, 1837, 1841
BseLI	CCNNNNNNNGG	1 cut(s)	851
BseMI	GCAATG	4 cut(s)	574, 765, 960, 1301
BseMII	CTCAG	4 cut(s)	1185, 1320, 1764, 1997
BseNI	ACTGG	4 cut(s)	667, 856, 1393, 1612
BseRI	GAGGAG	1 cut(s)	989
BseXI	GCAGC	4 cut(s)	118, 966, 1264, 2129
BseYI	CCCAGC	2 cut(s)	165, 409
BsiHKAI	GWGCWC	3 cut(s)	12, 104, 219
BsiSI	CCGG	6 cut(s)	87, 93, 147, 338, 518, 533
BslFI	GGGAC	3 cut(s)	426, 708, 1243
BslI	CCNNNNNNNGG	1 cut(s)	851
BsmAI	GTCTC	4 cut(s)	77, 193, 395, 694
BsmBI	CGTCTC	1 cut(s)	193
BsmFI	GGGAC	3 cut(s)	426, 708, 1243
Bso31I	GGTCTC	2 cut(s)	77, 694
Bsp119I	TTCGAA	4 cut(s)	27, 261, 446, 509
Bsp1286I	GDGCHC	3 cut(s)	12, 104, 219
Bsp13I	TCCGGA	2 cut(s)	92, 337
Bsp1407I	TGTACA	2 cut(s)	561, 1684
Bsp143I	GATC	8 cut(s)	288, 346, 1167, 1348, 1384, 1404, 1673, 2095
Bsp1720I	GCTNAGC	1 cut(s)	1194
BspACI	CCGC	3 cut(s)	5, 183, 1202
BspCNI	CTCAG	4 cut(s)	1186, 1321, 1765, 1998
BspEI	TCCGGA	2 cut(s)	92, 337
BspHI	TCATGA	1 cut(s)	657
BspLI	GGNNCC	4 cut(s)	59, 91, 633, 1603
BspMAI	CTGCAG	1 cut(s)	1294
BspMI	ACCTGC	1 cut(s)	1456
BspPI	GGATC	3 cut(s)	296, 354, 1681
BspQI	GCTCTTC	1 cut(s)	2025
BspT104I	TTCGAA	4 cut(s)	27, 261, 446, 509
BspTNI	GGTCTC	2 cut(s)	77, 694
BsrDI	GCAATG	4 cut(s)	574, 765, 960, 1301
BsrFI	RCCGGY	1 cut(s)	86
BsrGI	TGTACA	2 cut(s)	561, 1684
BsrI	ACTGG	4 cut(s)	667, 856, 1393, 1612
BssAI	RCCGGY	1 cut(s)	86
BssECI	CCNNGG	6 cut(s)	533, 932, 1047, 1260, 1456, 1597
BssMI	GATC	8 cut(s)	288, 346, 1167, 1348, 1384, 1404, 1673, 2095
BssNI	GRCGYC	2 cut(s)	150, 159
BssSI	CACGAG	2 cut(s)	380, 1171
BssT1I	CCWWGG	1 cut(s)	1047
Bst2BI	CACGAG	2 cut(s)	380, 1171
Bst2UI	CCWGG	4 cut(s)	933, 1262, 1311, 1898
Bst4CI	ACNGT	8 cut(s)	49, 501, 787, 1218, 1273, 1457, 1601, 1936
Bst6I	CTCTTC	5 cut(s)	24, 124, 428, 1693, 2025
BstACI	GRCGYC	2 cut(s)	150, 159
BstAUI	TGTACA	2 cut(s)	561, 1684
BstBAI	YACGTR	1 cut(s)	713
BstBI	TTCGAA	4 cut(s)	27, 261, 446, 509
BstC8I	GCNNGC	6 cut(s)	104, 441, 1418, 1463, 1770, 2037
BstDEI	CTNAG	6 cut(s)	579, 693, 1194, 1329, 1773, 2006
BstDSI	CCRYGG	2 cut(s)	1456, 1597
BstF5I	GGATG	7 cut(s)	676, 991, 1389, 1482, 1498, 1837, 1841
BstHHI	GCGC	1 cut(s)	1932
BstKTI	GATC	8 cut(s)	291, 349, 1170, 1351, 1387, 1407, 1676, 2098
BstMAI	GTCTC	4 cut(s)	77, 193, 395, 694
BstMBI	GATC	8 cut(s)	288, 346, 1167, 1348, 1384, 1404, 1673, 2095
BstMWI	GCNNNNNNNGC	3 cut(s)	156, 914, 2081
BstNI	CCWGG	4 cut(s)	933, 1262, 1311, 1898
BstNSI	RCATGY	2 cut(s)	859, 2039
BstPAI	GACNNNNGTC	1 cut(s)	1484
BstSCI	CCNGG	5 cut(s)	532, 931, 1260, 1309, 1896
BstSFI	CTRYAG	2 cut(s)	1290, 1400
BstV1I	GCAGC	4 cut(s)	118, 966, 1264, 2129
BstV2I	GAAGAC	1 cut(s)	1546
BstX2I	RGATCY	1 cut(s)	1404
BstYI	RGATCY	1 cut(s)	1404
BsuI	GTATCC	1 cut(s)	239
BtgI	CCRYGG	2 cut(s)	1456, 1597
BtrI	CACGTC	1 cut(s)	929
BtsCI	GGATG	7 cut(s)	676, 991, 1389, 1482, 1498, 1837, 1841
BtsIMutI	CAGTG	4 cut(s)	1214, 1605, 1640, 1932
BveI	ACCTGC	1 cut(s)	1456
Cac8I	GCNNGC	6 cut(s)	104, 441, 1418, 1463, 1770, 2037
CaiI	CAGNNNCTG	2 cut(s)	1277, 2090
CciI	TCATGA	1 cut(s)	657
CfoI	GCGC	1 cut(s)	1932
Cfr10I	RCCGGY	1 cut(s)	86
Cfr13I	GGNCC	4 cut(s)	1051, 1357, 1453, 1573
CseI	GACGC	3 cut(s)	139, 148, 563
CspCI	CAANNNNNGTGG	6 cut(s)	748, 783, 1438, 1473, 1990, 2025
CviAII	CATG	7 cut(s)	658, 856, 971, 1346, 1619, 1956, 2036
DdeI	CTNAG	6 cut(s)	579, 693, 1194, 1329, 1773, 2006
DpnI	GATC	8 cut(s)	290, 348, 1169, 1350, 1386, 1406, 1675, 2097
DpnII	GATC	8 cut(s)	288, 346, 1167, 1348, 1384, 1404, 1673, 2095
Eam1104I	CTCTTC	5 cut(s)	24, 124, 428, 1693, 2025
EarI	CTCTTC	5 cut(s)	24, 124, 428, 1693, 2025
EciI	GGCGGA	1 cut(s)	20
Ecl136II	GAGCTC	2 cut(s)	10, 217
Eco130I	CCWWGG	1 cut(s)	1047
Eco24I	GRGCYC	2 cut(s)	12, 219
Eco31I	GGTCTC	2 cut(s)	77, 694
Eco47I	GGWCC	4 cut(s)	1051, 1357, 1453, 1573
Eco53kI	GAGCTC	2 cut(s)	10, 217
Eco57I	CTGAAG	3 cut(s)	5, 657, 1145
EcoICRI	GAGCTC	2 cut(s)	10, 217
EcoRII	CCWGG	4 cut(s)	931, 1260, 1309, 1896
EcoT14I	CCWWGG	1 cut(s)	1047
EcoT38I	GRGCYC	2 cut(s)	12, 219
ErhI	CCWWGG	1 cut(s)	1047
Esp3I	CGTCTC	1 cut(s)	193
FaeI	CATG	7 cut(s)	661, 859, 974, 1349, 1622, 1959, 2039
FaqI	GGGAC	3 cut(s)	426, 708, 1243
FatI	CATG	7 cut(s)	657, 855, 970, 1345, 1618, 1955, 2035
FbaI	TGATCA	4 cut(s)	1167, 1348, 1384, 2095
Fnu4HI	GCNGC	5 cut(s)	107, 183, 980, 1278, 2118
FokI	GGATG	7 cut(s)	683, 998, 1376, 1489, 1505, 1824, 1828
FriOI	GRGCYC	2 cut(s)	12, 219
Fsp4HI	GCNGC	5 cut(s)	107, 183, 980, 1278, 2118
FspBI	CTAG	2 cut(s)	1205, 1544
FspI	TGCGCA	1 cut(s)	1931
GlaI	GCGC	1 cut(s)	1931
GluI	GCNGC	5 cut(s)	107, 183, 980, 1278, 2118
GsaI	CCCAGC	2 cut(s)	169, 413
HapII	CCGG	6 cut(s)	87, 93, 147, 338, 518, 533
HgaI	GACGC	3 cut(s)	139, 148, 563
HhaI	GCGC	1 cut(s)	1932
Hin1I	GRCGYC	2 cut(s)	150, 159
Hin1II	CATG	7 cut(s)	661, 859, 974, 1349, 1622, 1959, 2039
Hin6I	GCGC	1 cut(s)	1930
HinP1I	GCGC	1 cut(s)	1930
HinfI	GANTC	7 cut(s)	258, 283, 475, 593, 812, 1091, 1106
HpaII	CCGG	6 cut(s)	87, 93, 147, 338, 518, 533
HphI	GGTGA	4 cut(s)	154, 442, 497, 1394
Hpy166II	GTNNAC	2 cut(s)	1666, 1894
Hpy188I	TCNGA	7 cut(s)	80, 245, 277, 288, 1105, 1125, 2056
Hpy8I	GTNNAC	2 cut(s)	1666, 1894
Hpy99I	CGWCG	4 cut(s)	68, 155, 158, 197
HpyCH4III	ACNGT	8 cut(s)	49, 501, 787, 1218, 1273, 1457, 1601, 1936
HpyCH4IV	ACGT	4 cut(s)	712, 928, 1009, 1481
HpyF10VI	GCNNNNNNNGC	3 cut(s)	156, 914, 2081
HpyF3I	CTNAG	6 cut(s)	579, 693, 1194, 1329, 1773, 2006
HpySE526I	ACGT	4 cut(s)	712, 928, 1009, 1481
Hsp92I	GRCGYC	2 cut(s)	150, 159
Hsp92II	CATG	7 cut(s)	661, 859, 974, 1349, 1622, 1959, 2039
HspAI	GCGC	1 cut(s)	1930
Kpn2I	TCCGGA	2 cut(s)	92, 337
Ksp22I	TGATCA	4 cut(s)	1167, 1348, 1384, 2095
Kzo9I	GATC	8 cut(s)	288, 346, 1167, 1348, 1384, 1404, 1673, 2095
LguI	GCTCTTC	1 cut(s)	2025
LmnI	GCTCC	6 cut(s)	7, 15, 95, 214, 340, 976
Lsp1109I	GCAGC	4 cut(s)	118, 966, 1264, 2129
LweI	GCATC	6 cut(s)	976, 1108, 1135, 1429, 1846, 2062
MaeI	CTAG	2 cut(s)	1205, 1544
MaeII	ACGT	4 cut(s)	712, 928, 1009, 1481
MaeIII	GTNAC	4 cut(s)	160, 1582, 1960, 2077
MalI	GATC	8 cut(s)	290, 348, 1169, 1350, 1386, 1406, 1675, 2097
MboI	GATC	8 cut(s)	288, 346, 1167, 1348, 1384, 1404, 1673, 2095
MflI	RGATCY	1 cut(s)	1404
MhlI	GDGCHC	3 cut(s)	12, 104, 219
MluCI	AATT	9 cut(s)	360, 448, 511, 736, 879, 993, 1002, 1874, 1882
MlyI	GAGTC	3 cut(s)	277, 469, 602
MmeI	TCCRAC	1 cut(s)	223
MroI	TCCGGA	2 cut(s)	92, 337
MroXI	GAANNNNTTC	3 cut(s)	873, 2050, 2130
MseI	TTAA	4 cut(s)	627, 1229, 1815, 1983
MslI	CAYNNNNRTG	1 cut(s)	1623
MspI	CCGG	6 cut(s)	87, 93, 147, 338, 518, 533
MspR9I	CCNGG	5 cut(s)	534, 933, 1262, 1311, 1898
MvaI	CCWGG	4 cut(s)	933, 1262, 1311, 1898
MwoI	GCNNNNNNNGC	3 cut(s)	156, 914, 2081
NciI	CCSGG	1 cut(s)	534
NdeII	GATC	8 cut(s)	288, 346, 1167, 1348, 1384, 1404, 1673, 2095
NlaIII	CATG	7 cut(s)	661, 859, 974, 1349, 1622, 1959, 2039
NlaIV	GGNNCC	4 cut(s)	59, 91, 633, 1603
NmeAIII	GCCGAG	1 cut(s)	210
NmuCI	GTSAC	1 cut(s)	160
NsbI	TGCGCA	1 cut(s)	1931
NspI	RCATGY	2 cut(s)	859, 2039
NspV	TTCGAA	4 cut(s)	27, 261, 446, 509
PaeI	GCATGC	1 cut(s)	2039
PagI	TCATGA	1 cut(s)	657
PciSI	GCTCTTC	1 cut(s)	2025
PcsI	WCGNNNNNNNCGW	2 cut(s)	378, 910
PdmI	GAANNNNTTC	3 cut(s)	873, 2050, 2130
PfeI	GAWTC	4 cut(s)	258, 812, 1091, 1106
PkrI	GCNGC	5 cut(s)	108, 184, 981, 1279, 2119
PleI	GAGTC	3 cut(s)	277, 469, 601
PpsI	GAGTC	3 cut(s)	277, 469, 601
Ppu21I	YACGTR	1 cut(s)	713
PshAI	GACNNNNGTC	1 cut(s)	1484
Psp124BI	GAGCTC	2 cut(s)	12, 219
Psp1406I	AACGTT	1 cut(s)	1009
Psp6I	CCWGG	4 cut(s)	931, 1260, 1309, 1896
PspFI	CCCAGC	2 cut(s)	165, 409
PspGI	CCWGG	4 cut(s)	931, 1260, 1309, 1896
PspN4I	GGNNCC	4 cut(s)	59, 91, 633, 1603
PspPI	GGNCC	4 cut(s)	1051, 1357, 1453, 1573
PstI	CTGCAG	1 cut(s)	1294
PstNI	CAGNNNCTG	2 cut(s)	1277, 2090
PsuI	RGATCY	1 cut(s)	1404
RseI	CAYNNNNRTG	1 cut(s)	1623
SacI	GAGCTC	2 cut(s)	12, 219
SapI	GCTCTTC	1 cut(s)	2025
SaqAI	TTAA	4 cut(s)	627, 1229, 1815, 1983
SatI	GCNGC	5 cut(s)	107, 183, 980, 1278, 2118
Sau3AI	GATC	8 cut(s)	288, 346, 1167, 1348, 1384, 1404, 1673, 2095
Sau96I	GGNCC	4 cut(s)	1051, 1357, 1453, 1573
ScaI	AGTACT	3 cut(s)	296, 353, 1327
SchI	GAGTC	3 cut(s)	277, 469, 602
ScrFI	CCNGG	5 cut(s)	534, 933, 1262, 1311, 1898
SduI	GDGCHC	3 cut(s)	12, 104, 219
SfaNI	GCATC	6 cut(s)	976, 1108, 1135, 1429, 1846, 2062
SfcI	CTRYAG	2 cut(s)	1290, 1400
SfuI	TTCGAA	4 cut(s)	27, 261, 446, 509
SinI	GGWCC	4 cut(s)	1051, 1357, 1453, 1573
SmiMI	CAYNNNNRTG	1 cut(s)	1623
SmlI	CTYRAG	2 cut(s)	590, 1732
SmoI	CTYRAG	2 cut(s)	590, 1732
SphI	GCATGC	1 cut(s)	2039
Sse9I	AATT	9 cut(s)	360, 448, 511, 736, 879, 993, 1002, 1874, 1882
SsiI	CCGC	3 cut(s)	5, 183, 1202
SspI	AATATT	1 cut(s)	2050
SspMI	CTAG	2 cut(s)	1205, 1544
SstI	GAGCTC	2 cut(s)	12, 219
StyD4I	CCNGG	5 cut(s)	532, 931, 1260, 1309, 1896
StyI	CCWWGG	1 cut(s)	1047
TaaI	ACNGT	8 cut(s)	49, 501, 787, 1218, 1273, 1457, 1601, 1936
TaiI	ACGT	4 cut(s)	715, 931, 1012, 1484
TaqI	TCGA	9 cut(s)	27, 261, 446, 509, 615, 867, 1080, 1315, 1801
TaqII	GACCGA	1 cut(s)	317
TasI	AATT	9 cut(s)	360, 448, 511, 736, 879, 993, 1002, 1874, 1882
TatI	WGTACW	8 cut(s)	294, 351, 561, 837, 858, 1325, 1684, 1828
TauI	GCSGC	1 cut(s)	185
TfiI	GAWTC	4 cut(s)	258, 812, 1091, 1106
Tru1I	TTAA	4 cut(s)	627, 1229, 1815, 1983
Tru9I	TTAA	4 cut(s)	627, 1229, 1815, 1983
TscAI	CASTG	4 cut(s)	1221, 1612, 1647, 1939
TseFI	GTSAC	1 cut(s)	160
TseI	GCWGC	4 cut(s)	106, 979, 1277, 2117
Tsp45I	GTSAC	1 cut(s)	160
TspDTI	ATGAA	4 cut(s)	434, 646, 1170, 1881
TspGWI	ACGGA	3 cut(s)	70, 181, 413
TspRI	CASTG	4 cut(s)	1221, 1612, 1647, 1939
VpaK11BI	GGWCC	4 cut(s)	1051, 1357, 1453, 1573
XapI	RAATTY	3 cut(s)	448, 1002, 1874
XbaI	TCTAGA	1 cut(s)	1543
XceI	RCATGY	2 cut(s)	859, 2039
XcmI	CCANNNNNNNNNTGG	2 cut(s)	340, 1604
XmnI	GAANNNNTTC	3 cut(s)	873, 2050, 2130
XspI	CTAG	2 cut(s)	1205, 1544
ZrmI	AGTACT	3 cut(s)	296, 353, 1327

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.