Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0012265

Physical Location & Seq

Genomic Coordinates Forward (+)

52483686 .. 52488710

Gene Structure 5025 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G026320.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2040 bp

ATGGCGGAGCTCCCAATCCTTCAGTTCGAAGAGAAAATCATCGAAACAGTAGAGCGGAACCCCGTCGTTGTCATCATCGGAGAGACCGGTTCCGGAAAGAGCACGCAGCTCTCTCAAATCCTCCACAGAAGAGGCTACACCGACTCCGGCGTCGTCGGCGTCACTCAGCCTCGCCGTGTCGCCGCCGTCTCCGTCGCCAGACGAGTTTCGCAGGAGCTCAATGTTCGGCTCGGCGAGGAAGTCGGATACGCCATCAGATTCGAAGATAGAACTTCCGAAAGGACTCGGATCAAGTACTTGACCGATGGAGTTCTTCTTCGTGAGAGCCTATCCAATCCGGAGCTCGATCAGTACTCTGTCATTATACTCGATGAGGCTCACGAGAGAAGTCTCAACACGGACATATTGCTGGGACTGATGAAACGATTGGTGAAGAGGCGTGCTTCGAATTTGAAGGTTCTTATTACTTCGGCGACTCTTGATGGTGATAAAGTATCACAGTTCTTTTCGAATTGCCCGGTGCTGAATGTTCCGGGGAAGCTATACCCTGTGGAGATATTATACAGCAATGAGCGTCCTAAGAGCTATCTTGAGTCGTCTTTGAAAACAGCTCTCGATATACATATTAAGGAACCTGAAGGAGATGTCTTGATATTCATGACTGGGCAGGATGACATAGAGAAGTTGGTCTCTAAGTTAGAAGATAGAGTTAGAAGCCTGGAAGAAGGATCTGTCTTGGATGCCATAATCCTTCCTCTTCACGGTTCTCTGCAGCCTGAAATGCAGGTACGTGTATTTAGTCCCCCACCTCCTAATTGTAGGAGATTTATTGTTGCCACAAACATTGCTGAAACTTCTTTGACTGTTGATGGTGTTGTGTATGTTATTGATTCTGGTTATGTGAAGCAACGGCAGTACAACCCCTCAACTGGCATGTACTCCCTCGAAGTTGTTCAAATTAGCAAGGTACAAGCTAATCAACGAGCAGGACGAGCTGGAAGGACACGTCCTGGGAAATGCTATCGGTTATATCCTTCCGTTGCTTACCATGAGGAGCTGCTGGATGCAACAATTCCTGAAATTCAACGTTCTTCACTTGCTGGAAGTGTTCTTTATTTGAAATCCTTGGACCTCCCTGATATTGACATTCTCACATTCGATTTTCTTGATTCTCCTTCCTCTGAATCTTTACAAGATGCTCTGAAGCAGTTGTATCTCATTGATGCTATTGATGAAAATGGATTGATCACGAGAGTTGGGAAAACAATGGCTGAGCTTCCACTAGAACCTTCACTGTCAAGAACCTTAATGGAGGCAAATGAGTGTGGTTGCTTGTCCCAGGCTTTGACAGTTGCTGCTATGTTATCTGCAGAAACTACATTGCTTCCTGGTCGAAGGTTATTGCTACAGATCTATGAGTGCTGGCATCAAACTCACTATGACATTGATTGGTGTAAGGACCGTGGCTTGCAGGTACCTTTTTATAGTAGAGGGATGACATTTGTCAGGGATGTAAGGAAACAGCTATGTCAAATAATGCAAAAAATAGCAAAGGGATCATTAGATGTACAGACAAGCAGAAGAGGGAAACAAACCCAACAGGATTATAATAACTTGAGGAAGGCTTTATGTGTGGGGTATGCAAATCAGCTTGCTGAGAGAATGGTATTTCACAATGGCTATCGAACTTTAGGTTTTAAGCCCCAAGTAGTACAGGTGCATCCATCCTCTGTGCTAAAACCAGATGATGAAGGGAAATTTCCAAATTATGTCGTGTACCAGGAACTTATTGCCACTTCTCGTCCATACTTGCGCACTGTATGTGCTGTAAATGTTGCATGGGTTACACCCATTTTGAACAAGGTTAATAAACTAAACATCAACAAGCTGAGCGGTGGGAGTGGTCATATTGAAGAGCATGCAGAGGGAAATATTTCTGTTTTGCCAAAGAAAGATGCTGTTACAGCAGTTCCTGATGATCAAGAAAGTAAAATACAAGCAGCCAGAGAACGATTTCTTGCTCGTAAAGGAAAGAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010467 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

AAA_22 DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

679

Amino Acids

76.13

Weight (kDa)

8.16

Isoelectric Point (pI)

49.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	11 - 166	2.5e-08	DEAD/DEAH box helicase
AAA_22	PF13401	21 - 136	3.6e-07	AAA domain
Helicase_C	PF00271	206 - 335	1.1e-11	Helicase conserved C-terminal domain
WHD_HA2	PF04408	400 - 428	3.4e-08	Helicase associated domain (HA2), winged-helix
HA2_C	PF21010	429 - 462	6.4e-06	Helicase associated domain (HA2), ratchet-like
OB_NTP_bind	PF07717	539 - 619	2.4e-18	Oligonucleotide/oligosaccharide-binding (OB)-fold

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012265)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G27900 AT1G27900 AT1G27900
fragaria_vesca	FvH4_4g24140 FvH4_4g24140
malus_domestica	MD13G1115200.v1.1
prunus_persica	Prupe.1G232100_v2.0.a1 Prupe.1G232100_v2.0.a1
pyrus_communis	pycom13g09970
rosa_chinensis	RchiOBHm_Chr4g0430911
rosa_laevigata	RLG00000006939
rosa_multiflora	Rmu_sc0006263.1_g000011
rosa_roxburghii	Rroxscaffold_5G00372600
rosa_rugosa	Rorug04G0247300
rosa_samantha	Rh4AG302800 Rh4AG303700 Rh4BG310900 Rh4CG327300
rosa_wichuraiana	Rw4G026320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1608
AasI	GACNNNNNNGTC	1 cut(s)	149
Acc16I	TGCGCA	1 cut(s)	1814
Acc36I	ACCTGC	2 cut(s)	775, 1462
Acc65I	GGTACC	1 cut(s)	1474
AccB1I	GGYRCC	1 cut(s)	1474
AccBSI	CCGCTC	2 cut(s)	55, 1893
AccIII	TCCGGA	2 cut(s)	92, 337
AciI	CCGC	4 cut(s)	5, 55, 183, 1893
AclI	AACGTT	1 cut(s)	1087
AclWI	GGATC	3 cut(s)	296, 736, 1564
AcsI	RAATTY	3 cut(s)	448, 1080, 1757
AcuI	CTGAAG	3 cut(s)	5, 657, 1223
AcyI	GRCGYC	2 cut(s)	150, 159
AfiI	CCNNNNNNNGG	2 cut(s)	761, 929
AflIII	ACRYGT	2 cut(s)	790, 1004
AgeI	ACCGGT	1 cut(s)	86
AgsI	TTSAA	7 cut(s)	454, 604, 956, 1085, 1120, 1858, 1913
AjiI	CACGTC	1 cut(s)	1007
AjnI	CCWGG	5 cut(s)	717, 1009, 1338, 1387, 1779
Alw21I	GWGCWC	4 cut(s)	12, 104, 219, 345
Alw26I	GTCTC	4 cut(s)	77, 193, 395, 694
AlwI	GGATC	3 cut(s)	296, 736, 1564
AlwNI	CAGNNNCTG	2 cut(s)	1355, 1973
Aor13HI	TCCGGA	2 cut(s)	92, 337
ApeKI	GCWGC	5 cut(s)	106, 772, 1057, 1355, 2000
ApoI	RAATTY	3 cut(s)	448, 1080, 1757
AsiGI	ACCGGT	1 cut(s)	86
Asp700I	GAANNNNTTC	3 cut(s)	951, 1933, 2013
Asp718I	GGTACC	1 cut(s)	1474
AspLEI	GCGC	1 cut(s)	1815
AspS9I	GGNCC	2 cut(s)	1129, 1459
AsuC2I	CCSGG	2 cut(s)	518, 534
AsuHPI	GGTGA	2 cut(s)	442, 497
AsuII	TTCGAA	4 cut(s)	27, 261, 446, 509
AvaII	GGWCC	2 cut(s)	1129, 1459
BanI	GGYRCC	1 cut(s)	1474
BanII	GRGCYC	3 cut(s)	12, 219, 345
BauI	CACGAG	2 cut(s)	380, 1249
Bbv12I	GWGCWC	4 cut(s)	12, 104, 219, 345
BbvI	GCAGC	5 cut(s)	118, 784, 1044, 1342, 2012
BccI	CCATC	5 cut(s)	260, 299, 476, 863, 1732
BceAI	ACGGC	3 cut(s)	159, 170, 926
BciT130I	CCWGG	5 cut(s)	719, 1011, 1340, 1389, 1781
BciVI	GTATCC	1 cut(s)	239
BclI	TGATCA	2 cut(s)	1245, 1978
BcnI	CCSGG	2 cut(s)	518, 534
BcoDI	GTCTC	4 cut(s)	77, 193, 395, 694
BfaI	CTAG	1 cut(s)	1283
BfmI	CTRYAG	3 cut(s)	770, 1368, 1406
BfuAI	ACCTGC	2 cut(s)	775, 1462
BfuI	GTATCC	1 cut(s)	239
BglII	AGATCT	1 cut(s)	1410
BisI	GCNGC	6 cut(s)	107, 183, 773, 1058, 1356, 2001
BlpI	GCTNAGC	2 cut(s)	1272, 1889
BlsI	GCNGC	6 cut(s)	108, 184, 774, 1059, 1357, 2002
BmcAI	AGTACT	2 cut(s)	296, 353
Bme1390I	CCNGG	7 cut(s)	518, 534, 719, 1011, 1340, 1389, 1781
Bme18I	GGWCC	2 cut(s)	1129, 1459
BmgBI	CACGTC	1 cut(s)	1007
BmgT120I	GGNCC	2 cut(s)	1129, 1459
BmiI	GGNNCC	4 cut(s)	59, 91, 633, 1476
BmrFI	CCNGG	7 cut(s)	518, 534, 719, 1011, 1340, 1389, 1781
BmrI	ACTGGG	1 cut(s)	672
BmsI	GCATC	7 cut(s)	730, 1054, 1186, 1213, 1435, 1729, 1945
BmuI	ACTGGG	1 cut(s)	672
BoxI	GACNNNNGTC	1 cut(s)	1502
Bpu1102I	GCTNAGC	2 cut(s)	1272, 1889
Bpu14I	TTCGAA	4 cut(s)	27, 261, 446, 509
BpuEI	CTTGAG	2 cut(s)	611, 1636
BpuMI	CCSGG	2 cut(s)	518, 534
BsaAI	YACGTR	1 cut(s)	791
BsaHI	GRCGYC	2 cut(s)	150, 159
BsaI	GGTCTC	2 cut(s)	77, 694
BsaJI	CCNNGG	5 cut(s)	533, 1010, 1125, 1338, 1462
BsaWI	WCCGGW	3 cut(s)	86, 92, 337
BsaXI	ACNNNNNCTCC	2 cut(s)	128, 158
Bsc4I	CCNNNNNNNGG	2 cut(s)	761, 929
Bse118I	RCCGGY	1 cut(s)	86
Bse1I	ACTGG	2 cut(s)	667, 934
Bse3DI	GCAATG	3 cut(s)	574, 843, 1379
BseAI	TCCGGA	2 cut(s)	92, 337
BseBI	CCWGG	5 cut(s)	719, 1011, 1340, 1389, 1781
BseDI	CCNNGG	5 cut(s)	533, 1010, 1125, 1338, 1462
BseGI	GGATG	7 cut(s)	676, 745, 1069, 1500, 1516, 1720, 1724
BseLI	CCNNNNNNNGG	2 cut(s)	761, 929
BseMI	GCAATG	3 cut(s)	574, 843, 1379
BseMII	CTCAG	4 cut(s)	179, 1263, 1647, 1880
BseNI	ACTGG	2 cut(s)	667, 934
BseRI	GAGGAG	1 cut(s)	1067
BseXI	GCAGC	5 cut(s)	118, 784, 1044, 1342, 2012
BseYI	CCCAGC	1 cut(s)	409
BshNI	GGYRCC	1 cut(s)	1474
BshTI	ACCGGT	1 cut(s)	86
BsiHKAI	GWGCWC	4 cut(s)	12, 104, 219, 345
BsiSI	CCGG	6 cut(s)	87, 93, 147, 338, 518, 533
BslFI	GGGAC	3 cut(s)	426, 786, 1321
BslI	CCNNNNNNNGG	2 cut(s)	761, 929
BsmAI	GTCTC	4 cut(s)	77, 193, 395, 694
BsmBI	CGTCTC	1 cut(s)	193
BsmFI	GGGAC	3 cut(s)	426, 786, 1321
Bso31I	GGTCTC	2 cut(s)	77, 694
Bsp119I	TTCGAA	4 cut(s)	27, 261, 446, 509
Bsp1286I	GDGCHC	4 cut(s)	12, 104, 219, 345
Bsp13I	TCCGGA	2 cut(s)	92, 337
Bsp1407I	TGTACA	1 cut(s)	1567
Bsp143I	GATC	7 cut(s)	288, 346, 728, 1245, 1410, 1556, 1978
Bsp1720I	GCTNAGC	2 cut(s)	1272, 1889
BspACI	CCGC	4 cut(s)	5, 55, 183, 1893
BspCNI	CTCAG	4 cut(s)	178, 1264, 1648, 1881
BspEI	TCCGGA	2 cut(s)	92, 337
BspHI	TCATGA	1 cut(s)	657
BspLI	GGNNCC	4 cut(s)	59, 91, 633, 1476
BspMAI	CTGCAG	2 cut(s)	774, 1372
BspMI	ACCTGC	2 cut(s)	775, 1462
BspPI	GGATC	3 cut(s)	296, 736, 1564
BspQI	GCTCTTC	1 cut(s)	1908
BspT104I	TTCGAA	4 cut(s)	27, 261, 446, 509
BspT107I	GGYRCC	1 cut(s)	1474
BspTNI	GGTCTC	2 cut(s)	77, 694
BsrBI	CCGCTC	2 cut(s)	55, 1893
BsrDI	GCAATG	3 cut(s)	574, 843, 1379
BsrFI	RCCGGY	1 cut(s)	86
BsrGI	TGTACA	1 cut(s)	1567
BsrI	ACTGG	2 cut(s)	667, 934
BssAI	RCCGGY	1 cut(s)	86
BssECI	CCNNGG	5 cut(s)	533, 1010, 1125, 1338, 1462
BssMI	GATC	7 cut(s)	288, 346, 728, 1245, 1410, 1556, 1978
BssNI	GRCGYC	2 cut(s)	150, 159
BssSI	CACGAG	2 cut(s)	380, 1249
BssT1I	CCWWGG	1 cut(s)	1125
Bst2BI	CACGAG	2 cut(s)	380, 1249
Bst2UI	CCWGG	5 cut(s)	719, 1011, 1340, 1389, 1781
Bst4CI	ACNGT	8 cut(s)	49, 501, 764, 865, 1296, 1351, 1463, 1819
Bst6I	CTCTTC	6 cut(s)	24, 124, 428, 762, 1576, 1908
BstACI	GRCGYC	2 cut(s)	150, 159
BstAUI	TGTACA	1 cut(s)	1567
BstBAI	YACGTR	1 cut(s)	791
BstBI	TTCGAA	4 cut(s)	27, 261, 446, 509
BstC8I	GCNNGC	6 cut(s)	104, 441, 1424, 1469, 1653, 1920
BstDEI	CTNAG	6 cut(s)	165, 579, 693, 1272, 1656, 1889
BstDSI	CCRYGG	1 cut(s)	1462
BstF5I	GGATG	7 cut(s)	676, 745, 1069, 1500, 1516, 1720, 1724
BstHHI	GCGC	1 cut(s)	1815
BstKTI	GATC	7 cut(s)	291, 349, 731, 1248, 1413, 1559, 1981
BstMAI	GTCTC	4 cut(s)	77, 193, 395, 694
BstMBI	GATC	7 cut(s)	288, 346, 728, 1245, 1410, 1556, 1978
BstMWI	GCNNNNNNNGC	4 cut(s)	156, 781, 992, 1964
BstNI	CCWGG	5 cut(s)	719, 1011, 1340, 1389, 1781
BstNSI	RCATGY	2 cut(s)	937, 1922
BstPAI	GACNNNNGTC	1 cut(s)	1502
BstSCI	CCNGG	7 cut(s)	516, 532, 717, 1009, 1338, 1387, 1779
BstSFI	CTRYAG	3 cut(s)	770, 1368, 1406
BstV1I	GCAGC	5 cut(s)	118, 784, 1044, 1342, 2012
BstX2I	RGATCY	2 cut(s)	728, 1410
BstYI	RGATCY	2 cut(s)	728, 1410
BsuI	GTATCC	1 cut(s)	239
BtgI	CCRYGG	1 cut(s)	1462
BtrI	CACGTC	1 cut(s)	1007
BtsCI	GGATG	7 cut(s)	676, 745, 1069, 1500, 1516, 1720, 1724
BtsIMutI	CAGTG	2 cut(s)	1292, 1815
BveI	ACCTGC	2 cut(s)	775, 1462
Cac8I	GCNNGC	6 cut(s)	104, 441, 1424, 1469, 1653, 1920
CaiI	CAGNNNCTG	2 cut(s)	1355, 1973
CciI	TCATGA	1 cut(s)	657
CfoI	GCGC	1 cut(s)	1815
Cfr10I	RCCGGY	1 cut(s)	86
Cfr13I	GGNCC	2 cut(s)	1129, 1459
CseI	GACGC	3 cut(s)	139, 148, 563
CspAI	ACCGGT	1 cut(s)	86
CspCI	CAANNNNNGTGG	2 cut(s)	826, 861
CviAII	CATG	5 cut(s)	658, 934, 1049, 1839, 1919
DdeI	CTNAG	6 cut(s)	165, 579, 693, 1272, 1656, 1889
DpnI	GATC	7 cut(s)	290, 348, 730, 1247, 1412, 1558, 1980
DpnII	GATC	7 cut(s)	288, 346, 728, 1245, 1410, 1556, 1978
DrdI	GACNNNNNNGTC	1 cut(s)	149
DseDI	GACNNNNNNGTC	1 cut(s)	149
Eam1104I	CTCTTC	6 cut(s)	24, 124, 428, 762, 1576, 1908
EarI	CTCTTC	6 cut(s)	24, 124, 428, 762, 1576, 1908
EciI	GGCGGA	1 cut(s)	20
Ecl136II	GAGCTC	3 cut(s)	10, 217, 343
Eco130I	CCWWGG	1 cut(s)	1125
Eco24I	GRGCYC	3 cut(s)	12, 219, 345
Eco31I	GGTCTC	2 cut(s)	77, 694
Eco47I	GGWCC	2 cut(s)	1129, 1459
Eco53kI	GAGCTC	3 cut(s)	10, 217, 343
Eco57I	CTGAAG	3 cut(s)	5, 657, 1223
EcoICRI	GAGCTC	3 cut(s)	10, 217, 343
EcoRII	CCWGG	5 cut(s)	717, 1009, 1338, 1387, 1779
EcoT14I	CCWWGG	1 cut(s)	1125
EcoT38I	GRGCYC	3 cut(s)	12, 219, 345
ErhI	CCWWGG	1 cut(s)	1125
Esp3I	CGTCTC	1 cut(s)	193
FaeI	CATG	5 cut(s)	661, 937, 1052, 1842, 1922
FaqI	GGGAC	3 cut(s)	426, 786, 1321
FatI	CATG	5 cut(s)	657, 933, 1048, 1838, 1918
FbaI	TGATCA	2 cut(s)	1245, 1978
Fnu4HI	GCNGC	6 cut(s)	107, 183, 773, 1058, 1356, 2001
FokI	GGATG	7 cut(s)	683, 752, 1076, 1507, 1523, 1707, 1711
FriOI	GRGCYC	3 cut(s)	12, 219, 345
Fsp4HI	GCNGC	6 cut(s)	107, 183, 773, 1058, 1356, 2001
FspBI	CTAG	1 cut(s)	1283
FspI	TGCGCA	1 cut(s)	1814
GlaI	GCGC	1 cut(s)	1814
GluI	GCNGC	6 cut(s)	107, 183, 773, 1058, 1356, 2001
GsaI	CCCAGC	1 cut(s)	413
HapII	CCGG	6 cut(s)	87, 93, 147, 338, 518, 533
HgaI	GACGC	3 cut(s)	139, 148, 563
HhaI	GCGC	1 cut(s)	1815
Hin1I	GRCGYC	2 cut(s)	150, 159
Hin1II	CATG	5 cut(s)	661, 937, 1052, 1842, 1922
Hin6I	GCGC	1 cut(s)	1813
HinP1I	GCGC	1 cut(s)	1813
HinfI	GANTC	8 cut(s)	143, 258, 283, 475, 593, 890, 1169, 1184
HpaII	CCGG	6 cut(s)	87, 93, 147, 338, 518, 533
HphI	GGTGA	2 cut(s)	442, 497
Hpy166II	GTNNAC	1 cut(s)	1777
Hpy188I	TCNGA	7 cut(s)	80, 245, 257, 277, 288, 1183, 1203
Hpy8I	GTNNAC	1 cut(s)	1777
Hpy99I	CGWCG	4 cut(s)	68, 155, 158, 197
HpyCH4III	ACNGT	8 cut(s)	49, 501, 764, 865, 1296, 1351, 1463, 1819
HpyCH4IV	ACGT	3 cut(s)	790, 1006, 1087
HpyF10VI	GCNNNNNNNGC	4 cut(s)	156, 781, 992, 1964
HpyF3I	CTNAG	6 cut(s)	165, 579, 693, 1272, 1656, 1889
HpySE526I	ACGT	3 cut(s)	790, 1006, 1087
Hsp92I	GRCGYC	2 cut(s)	150, 159
Hsp92II	CATG	5 cut(s)	661, 937, 1052, 1842, 1922
HspAI	GCGC	1 cut(s)	1813
Kpn2I	TCCGGA	2 cut(s)	92, 337
KpnI	GGTACC	1 cut(s)	1478
Ksp22I	TGATCA	2 cut(s)	1245, 1978
Kzo9I	GATC	7 cut(s)	288, 346, 728, 1245, 1410, 1556, 1978
LguI	GCTCTTC	1 cut(s)	1908
LmnI	GCTCC	5 cut(s)	7, 15, 214, 340, 1054
Lsp1109I	GCAGC	5 cut(s)	118, 784, 1044, 1342, 2012
LweI	GCATC	7 cut(s)	730, 1054, 1186, 1213, 1435, 1729, 1945
MaeI	CTAG	1 cut(s)	1283
MaeII	ACGT	3 cut(s)	790, 1006, 1087
MaeIII	GTNAC	3 cut(s)	160, 1843, 1960
MalI	GATC	7 cut(s)	290, 348, 730, 1247, 1412, 1558, 1980
MbiI	CCGCTC	2 cut(s)	55, 1893
MboI	GATC	7 cut(s)	288, 346, 728, 1245, 1410, 1556, 1978
MflI	RGATCY	2 cut(s)	728, 1410
MhlI	GDGCHC	4 cut(s)	12, 104, 219, 345
MluCI	AATT	8 cut(s)	448, 511, 814, 957, 1071, 1080, 1757, 1765
MlyI	GAGTC	4 cut(s)	137, 277, 469, 602
MmeI	TCCRAC	1 cut(s)	223
MroI	TCCGGA	2 cut(s)	92, 337
MroXI	GAANNNNTTC	3 cut(s)	951, 1933, 2013
MseI	TTAA	4 cut(s)	627, 1307, 1698, 1866
MspI	CCGG	6 cut(s)	87, 93, 147, 338, 518, 533
MspR9I	CCNGG	7 cut(s)	518, 534, 719, 1011, 1340, 1389, 1781
MvaI	CCWGG	5 cut(s)	719, 1011, 1340, 1389, 1781
MwoI	GCNNNNNNNGC	4 cut(s)	156, 781, 992, 1964
NciI	CCSGG	2 cut(s)	518, 534
NdeII	GATC	7 cut(s)	288, 346, 728, 1245, 1410, 1556, 1978
NlaIII	CATG	5 cut(s)	661, 937, 1052, 1842, 1922
NlaIV	GGNNCC	4 cut(s)	59, 91, 633, 1476
NmeAIII	GCCGAG	1 cut(s)	210
NmuCI	GTSAC	1 cut(s)	160
NsbI	TGCGCA	1 cut(s)	1814
NspI	RCATGY	2 cut(s)	937, 1922
NspV	TTCGAA	4 cut(s)	27, 261, 446, 509
PaeI	GCATGC	1 cut(s)	1922
PagI	TCATGA	1 cut(s)	657
PciSI	GCTCTTC	1 cut(s)	1908
PcsI	WCGNNNNNNNCGW	1 cut(s)	988
PdmI	GAANNNNTTC	3 cut(s)	951, 1933, 2013
PfeI	GAWTC	4 cut(s)	258, 890, 1169, 1184
PinAI	ACCGGT	1 cut(s)	86
PkrI	GCNGC	6 cut(s)	108, 184, 774, 1059, 1357, 2002
PleI	GAGTC	4 cut(s)	137, 277, 469, 601
PpsI	GAGTC	4 cut(s)	137, 277, 469, 601
Ppu21I	YACGTR	1 cut(s)	791
PshAI	GACNNNNGTC	1 cut(s)	1502
PsiI	TTATAA	1 cut(s)	1608
Psp124BI	GAGCTC	3 cut(s)	12, 219, 345
Psp1406I	AACGTT	1 cut(s)	1087
Psp6I	CCWGG	5 cut(s)	717, 1009, 1338, 1387, 1779
PspFI	CCCAGC	1 cut(s)	409
PspGI	CCWGG	5 cut(s)	717, 1009, 1338, 1387, 1779
PspN4I	GGNNCC	4 cut(s)	59, 91, 633, 1476
PspPI	GGNCC	2 cut(s)	1129, 1459
PstI	CTGCAG	2 cut(s)	774, 1372
PstNI	CAGNNNCTG	2 cut(s)	1355, 1973
PsuI	RGATCY	2 cut(s)	728, 1410
SacI	GAGCTC	3 cut(s)	12, 219, 345
SapI	GCTCTTC	1 cut(s)	1908
SaqAI	TTAA	4 cut(s)	627, 1307, 1698, 1866
SatI	GCNGC	6 cut(s)	107, 183, 773, 1058, 1356, 2001
Sau3AI	GATC	7 cut(s)	288, 346, 728, 1245, 1410, 1556, 1978
Sau96I	GGNCC	2 cut(s)	1129, 1459
ScaI	AGTACT	2 cut(s)	296, 353
SchI	GAGTC	4 cut(s)	137, 277, 469, 602
ScrFI	CCNGG	7 cut(s)	518, 534, 719, 1011, 1340, 1389, 1781
SduI	GDGCHC	4 cut(s)	12, 104, 219, 345
SfaNI	GCATC	7 cut(s)	730, 1054, 1186, 1213, 1435, 1729, 1945
SfcI	CTRYAG	3 cut(s)	770, 1368, 1406
SfuI	TTCGAA	4 cut(s)	27, 261, 446, 509
SinI	GGWCC	2 cut(s)	1129, 1459
SmlI	CTYRAG	2 cut(s)	590, 1615
SmoI	CTYRAG	2 cut(s)	590, 1615
SphI	GCATGC	1 cut(s)	1922
Sse9I	AATT	8 cut(s)	448, 511, 814, 957, 1071, 1080, 1757, 1765
SsiI	CCGC	4 cut(s)	5, 55, 183, 1893
SspI	AATATT	1 cut(s)	1933
SspMI	CTAG	1 cut(s)	1283
SstI	GAGCTC	3 cut(s)	12, 219, 345
StyD4I	CCNGG	7 cut(s)	516, 532, 717, 1009, 1338, 1387, 1779
StyI	CCWWGG	1 cut(s)	1125
TaaI	ACNGT	8 cut(s)	49, 501, 764, 865, 1296, 1351, 1463, 1819
TaiI	ACGT	3 cut(s)	793, 1009, 1090
TaqII	GACCGA	1 cut(s)	317
TasI	AATT	8 cut(s)	448, 511, 814, 957, 1071, 1080, 1757, 1765
TatI	WGTACW	6 cut(s)	294, 351, 915, 936, 1567, 1711
TauI	GCSGC	1 cut(s)	185
TfiI	GAWTC	4 cut(s)	258, 890, 1169, 1184
Tru1I	TTAA	4 cut(s)	627, 1307, 1698, 1866
Tru9I	TTAA	4 cut(s)	627, 1307, 1698, 1866
TscAI	CASTG	2 cut(s)	1299, 1822
TseFI	GTSAC	1 cut(s)	160
TseI	GCWGC	5 cut(s)	106, 772, 1057, 1355, 2000
Tsp45I	GTSAC	1 cut(s)	160
TspDTI	ATGAA	4 cut(s)	434, 646, 1248, 1764
TspGWI	ACGGA	3 cut(s)	181, 413, 1027
TspRI	CASTG	2 cut(s)	1299, 1822
VpaK11BI	GGWCC	2 cut(s)	1129, 1459
XapI	RAATTY	3 cut(s)	448, 1080, 1757
XceI	RCATGY	2 cut(s)	937, 1922
XmnI	GAANNNNTTC	3 cut(s)	951, 1933, 2013
XspI	CTAG	1 cut(s)	1283
ZrmI	AGTACT	2 cut(s)	296, 353

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.