MD13G1121000.v1.1

Nuclear transport factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0013913
Physical Location & Seq
Forward (+)
8890265 .. 8892830
2566 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1121000.v1.1.491

Sequence Viewer

Length: 372 bp
ATGGATCCAGACGCACTTTCCAAGGCCTTCGTGGAGCACTACTACACGTTGTTCGACGCCAACCGCGGCGCCTTGGCGAATCTGTACCAGGAAACCTCGATGTTGACCTTCGAAGGTCAGAAGATCCAAGGGTCACAAAGCATCGTGGCCAAGCTCACCAGCCTCCCTTTCCAGCAGTGCCAGCACAGCATCACCACCGTCGATTGCCAGCCCTCTGGCCCCGCCGGCGGCATGCTCGTCTTCGTTAGCGGCAATCTCCAGCTCGCCGGCGAACAGCACGCTCTCAAGTTCAGCCAGATGTTCCATCTGATGCCGACTCAACAGGGAAGCTTCTACGTGCTCAATGACATTTTCCGGTTGAACTATGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NTF2

Protein Analysis

124

Amino Acids

13.64

Weight (kDa)

5.67

Isoelectric Point (pI)

38.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NTF2 PF02136 7 - 120 2.9e-39 Nuclear transport factor 2 (NTF2) domain
NXF_NTF2 PF22602 7 - 37 8.3e-06 Nuclear RNA export factor, NTF2 domain
NXF_NTF2 PF22602 43 - 118 4.3e-06 Nuclear RNA export factor, NTF2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013913)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G27310
fragaria_vesca FvH4_4g23580
malus_domestica MD13G1121000.v1.1 MD16G1121000.v1.1
prunus_persica Prupe.1G224200_v2.0.a1
pyrus_communis pycom13g10530 pycom16g10360
rosa_chinensis RchiOBHm_Chr4g0430001
rosa_laevigata RLG00000007015
rosa_multiflora Rmu_ssc0000150.1_g000036
rosa_roxburghii Rroxscaffold_5G00371810
rosa_rugosa Rorug04G0239800
rosa_samantha Rh4AG296300 Rh4BG302800 Rh4CG318300 Rh4DG300100
rosa_wichuraiana Rw4G025670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 68
AccII CGCG 1 cut(s) 66
AciI CCGC 5 cut(s) 64, 66, 222, 228, 249
AclWI GGATC 2 cut(s) 12, 118
AcoI YGGCCR 1 cut(s) 147
AcyI GRCGYC 2 cut(s) 57, 69
AfaI GTAC 1 cut(s) 86
AfiI CCNNNNNNNGG 1 cut(s) 227
AflIII ACRYGT 1 cut(s) 45
AgsI TTSAA 1 cut(s) 361
AjnI CCWGG 1 cut(s) 87
AluBI AGCT 3 cut(s) 154, 262, 330
AluI AGCT 3 cut(s) 154, 262, 330
Alw21I GWGCWC 2 cut(s) 39, 342
AlwI GGATC 2 cut(s) 12, 118
AoxI GGCC 3 cut(s) 24, 147, 217
AspLEI GCGC 1 cut(s) 71
AspS9I GGNCC 1 cut(s) 218
AsuHPI GGTGA 2 cut(s) 148, 184
AsuII TTCGAA 1 cut(s) 111
BalI TGGCCA 1 cut(s) 149
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 68
BbsI GAAGAC 1 cut(s) 232
Bbv12I GWGCWC 2 cut(s) 39, 342
BccI CCATC 1 cut(s) 312
BciT130I CCWGG 1 cut(s) 89
BfoI RGCGCY 1 cut(s) 72
BglI GCCNNNNNGGC 1 cut(s) 225
BisI GCNGC 3 cut(s) 67, 229, 250
BlsI GCNGC 3 cut(s) 68, 230, 251
Bme1390I CCNGG 1 cut(s) 89
BmgT120I GGNCC 1 cut(s) 218
BmiI GGNNCC 3 cut(s) 6, 70, 220
BmrFI CCNGG 1 cut(s) 89
BmsI GCATC 3 cut(s) 150, 198, 300
BpiI GAAGAC 1 cut(s) 232
BpmI CTGGAG 1 cut(s) 242
Bpu14I TTCGAA 1 cut(s) 111
BpuEI CTTGAG 1 cut(s) 269
BsaAI YACGTR 1 cut(s) 337
BsaHI GRCGYC 2 cut(s) 57, 69
BsaJI CCNNGG 4 cut(s) 21, 64, 72, 127
BsaWI WCCGGW 1 cut(s) 354
Bsc4I CCNNNNNNNGG 1 cut(s) 227
Bse118I RCCGGY 2 cut(s) 224, 266
BseBI CCWGG 1 cut(s) 89
BseDI CCNNGG 4 cut(s) 21, 64, 72, 127
BseLI CCNNNNNNNGG 1 cut(s) 227
Bsh1236I CGCG 1 cut(s) 66
BshFI GGCC 3 cut(s) 26, 149, 219
BshNI GGYRCC 1 cut(s) 68
BsiHKAI GWGCWC 2 cut(s) 39, 342
BsiSI CCGG 3 cut(s) 225, 267, 355
BslI CCNNNNNNNGG 1 cut(s) 227
BsnI GGCC 3 cut(s) 26, 149, 219
Bsp119I TTCGAA 1 cut(s) 111
Bsp1286I GDGCHC 2 cut(s) 39, 342
Bsp143I GATC 2 cut(s) 4, 123
BspACI CCGC 5 cut(s) 64, 66, 222, 228, 249
BspANI GGCC 3 cut(s) 26, 149, 219
BspFNI CGCG 1 cut(s) 66
BspLI GGNNCC 3 cut(s) 6, 70, 220
BspPI GGATC 2 cut(s) 12, 118
BspT104I TTCGAA 1 cut(s) 111
BspT107I GGYRCC 1 cut(s) 68
BsrFI RCCGGY 2 cut(s) 224, 266
BssAI RCCGGY 2 cut(s) 224, 266
BssECI CCNNGG 4 cut(s) 21, 64, 72, 127
BssMI GATC 2 cut(s) 4, 123
BssNI GRCGYC 2 cut(s) 57, 69
BssT1I CCWWGG 3 cut(s) 21, 72, 127
Bst2UI CCWGG 1 cut(s) 89
Bst4CI ACNGT 1 cut(s) 199
BstACI GRCGYC 2 cut(s) 57, 69
BstBAI YACGTR 1 cut(s) 337
BstBI TTCGAA 1 cut(s) 111
BstC8I GCNNGC 7 cut(s) 182, 209, 226, 233, 264, 268, 279
BstDSI CCRYGG 1 cut(s) 64
BstFNI CGCG 1 cut(s) 66
BstH2I RGCGCY 1 cut(s) 72
BstHHI GCGC 1 cut(s) 71
BstKTI GATC 2 cut(s) 7, 126
BstMBI GATC 2 cut(s) 4, 123
BstMWI GCNNNNNNNGC 3 cut(s) 181, 186, 225
BstNI CCWGG 1 cut(s) 89
BstNSI RCATGY 1 cut(s) 235
BstSCI CCNGG 1 cut(s) 87
BstUI CGCG 1 cut(s) 66
BstV2I GAAGAC 1 cut(s) 232
BstX2I RGATCY 2 cut(s) 4, 123
BstXI CCANNNNNNTGG 1 cut(s) 215
BstYI RGATCY 2 cut(s) 4, 123
BsuRI GGCC 3 cut(s) 26, 149, 219
BtgI CCRYGG 1 cut(s) 64
BtsI GCAGTG 1 cut(s) 182
BtsIMutI CAGTG 1 cut(s) 182
Cac8I GCNNGC 7 cut(s) 182, 209, 226, 233, 264, 268, 279
CfoI GCGC 1 cut(s) 71
Cfr10I RCCGGY 2 cut(s) 224, 266
Cfr13I GGNCC 1 cut(s) 218
Cfr42I CCGCGG 1 cut(s) 67
CseI GACGC 2 cut(s) 20, 65
Csp6I GTAC 1 cut(s) 85
CviAII CATG 1 cut(s) 232
CviJI RGCY 9 cut(s) 26, 149, 154, 162, 211, 219, 262, 294, 330
CviKI_1 RGCY 9 cut(s) 26, 149, 154, 162, 211, 219, 262, 294, 330
CviQI GTAC 1 cut(s) 85
DinI GGCGCC 1 cut(s) 70
DpnI GATC 2 cut(s) 6, 125
DpnII GATC 2 cut(s) 4, 123
EaeI YGGCCR 1 cut(s) 147
Eco130I CCWWGG 3 cut(s) 21, 72, 127
Eco147I AGGCCT 1 cut(s) 26
EcoRII CCWGG 1 cut(s) 87
EcoT14I CCWWGG 3 cut(s) 21, 72, 127
EgeI GGCGCC 1 cut(s) 70
EheI GGCGCC 1 cut(s) 70
ErhI CCWWGG 3 cut(s) 21, 72, 127
FaeI CATG 1 cut(s) 235
FaiI YATR 2 cut(s) 233, 366
FatI CATG 1 cut(s) 231
FauI CCCGC 1 cut(s) 229
Fnu4HI GCNGC 3 cut(s) 67, 229, 250
Fsp4HI GCNGC 3 cut(s) 67, 229, 250
GlaI GCGC 1 cut(s) 70
GluI GCNGC 3 cut(s) 67, 229, 250
GsuI CTGGAG 1 cut(s) 242
HaeII RGCGCY 1 cut(s) 72
HaeIII GGCC 3 cut(s) 26, 149, 219
HapII CCGG 3 cut(s) 225, 267, 355
HgaI GACGC 2 cut(s) 20, 65
HhaI GCGC 1 cut(s) 71
Hin1I GRCGYC 2 cut(s) 57, 69
Hin1II CATG 1 cut(s) 235
Hin6I GCGC 1 cut(s) 69
HinP1I GCGC 1 cut(s) 69
HincII GTYRAC 1 cut(s) 105
HindII GTYRAC 1 cut(s) 105
HindIII AAGCTT 1 cut(s) 328
HinfI GANTC 2 cut(s) 79, 316
HpaII CCGG 3 cut(s) 225, 267, 355
HphI GGTGA 2 cut(s) 148, 184
Hpy166II GTNNAC 1 cut(s) 105
Hpy188I TCNGA 2 cut(s) 120, 309
Hpy188III TCNNGA 1 cut(s) 8
Hpy8I GTNNAC 1 cut(s) 105
Hpy99I CGWCG 2 cut(s) 59, 203
HpyAV CCTTC 3 cut(s) 37, 107, 118
HpyCH4III ACNGT 1 cut(s) 199
HpyCH4IV ACGT 2 cut(s) 47, 336
HpyF10VI GCNNNNNNNGC 3 cut(s) 181, 186, 225
HpySE526I ACGT 2 cut(s) 47, 336
Hsp92I GRCGYC 2 cut(s) 57, 69
Hsp92II CATG 1 cut(s) 235
HspAI GCGC 1 cut(s) 69
KasI GGCGCC 1 cut(s) 68
KroI GCCGGC 2 cut(s) 224, 266
KroNI GCCGGC 2 cut(s) 226, 268
KspI CCGCGG 1 cut(s) 67
Kzo9I GATC 2 cut(s) 4, 123
LmnI GCTCC 1 cut(s) 34
LweI GCATC 3 cut(s) 150, 198, 300
MaeII ACGT 2 cut(s) 47, 336
MaeIII GTNAC 1 cut(s) 132
MalI GATC 2 cut(s) 6, 125
MboI GATC 2 cut(s) 4, 123
MboII GAAGA 2 cut(s) 133, 232
MflI RGATCY 2 cut(s) 4, 123
MhlI GDGCHC 2 cut(s) 39, 342
MlsI TGGCCA 1 cut(s) 149
MluNI TGGCCA 1 cut(s) 149
Mly113I GGCGCC 1 cut(s) 69
MlyI GAGTC 1 cut(s) 310
MnlI CCTC 3 cut(s) 106, 173, 223
Mox20I TGGCCA 1 cut(s) 149
MreI CGCCGGCG 2 cut(s) 224, 266
MroNI GCCGGC 2 cut(s) 224, 266
MscI TGGCCA 1 cut(s) 149
Msp20I TGGCCA 1 cut(s) 149
MspA1I CMGCKG 1 cut(s) 66
MspI CCGG 3 cut(s) 225, 267, 355
MspR9I CCNGG 1 cut(s) 89
MvaI CCWGG 1 cut(s) 89
MvnI CGCG 1 cut(s) 66
MwoI GCNNNNNNNGC 3 cut(s) 181, 186, 225
NaeI GCCGGC 2 cut(s) 226, 268
NarI GGCGCC 1 cut(s) 69
NdeII GATC 2 cut(s) 4, 123
NgoMIV GCCGGC 2 cut(s) 224, 266
NlaIII CATG 1 cut(s) 235
NlaIV GGNNCC 3 cut(s) 6, 70, 220
NmuCI GTSAC 1 cut(s) 132
NspI RCATGY 1 cut(s) 235
NspV TTCGAA 1 cut(s) 111
PaeI GCATGC 1 cut(s) 235
PceI AGGCCT 1 cut(s) 26
PdiI GCCGGC 2 cut(s) 226, 268
PfeI GAWTC 1 cut(s) 79
PkrI GCNGC 3 cut(s) 68, 230, 251
PleI GAGTC 1 cut(s) 310
PluTI GGCGCC 1 cut(s) 72
PpsI GAGTC 1 cut(s) 310
Ppu21I YACGTR 1 cut(s) 337
Psp6I CCWGG 1 cut(s) 87
PspGI CCWGG 1 cut(s) 87
PspN4I GGNNCC 3 cut(s) 6, 70, 220
PspPI GGNCC 1 cut(s) 218
PsuI RGATCY 2 cut(s) 4, 123
RsaI GTAC 1 cut(s) 86
RsaNI GTAC 1 cut(s) 85
SacII CCGCGG 1 cut(s) 67
SatI GCNGC 3 cut(s) 67, 229, 250
Sau3AI GATC 2 cut(s) 4, 123
Sau96I GGNCC 1 cut(s) 218
SchI GAGTC 1 cut(s) 310
ScrFI CCNGG 1 cut(s) 89
SduI GDGCHC 2 cut(s) 39, 342
SetI ASST 8 cut(s) 50, 98, 110, 118, 156, 264, 332, 339
SfaNI GCATC 3 cut(s) 150, 198, 300
SfoI GGCGCC 1 cut(s) 70
Sfr303I CCGCGG 1 cut(s) 67
SfuI TTCGAA 1 cut(s) 111
SgrAI CRCCGGYG 2 cut(s) 224, 266
SgrBI CCGCGG 1 cut(s) 67
SmlI CTYRAG 1 cut(s) 284
SmoI CTYRAG 1 cut(s) 284
SphI GCATGC 1 cut(s) 235
SseBI AGGCCT 1 cut(s) 26
SsiI CCGC 5 cut(s) 64, 66, 222, 228, 249
SspDI GGCGCC 1 cut(s) 68
StuI AGGCCT 1 cut(s) 26
StyD4I CCNGG 1 cut(s) 87
StyI CCWWGG 3 cut(s) 21, 72, 127
TaaI ACNGT 1 cut(s) 199
TaiI ACGT 2 cut(s) 50, 339
TaqI TCGA 4 cut(s) 54, 98, 111, 201
TauI GCSGC 3 cut(s) 69, 231, 252
TfiI GAWTC 1 cut(s) 79
TscAI CASTG 1 cut(s) 182
TseFI GTSAC 1 cut(s) 132
Tsp45I GTSAC 1 cut(s) 132
TspRI CASTG 1 cut(s) 182
XceI RCATGY 1 cut(s) 235
XcmI CCANNNNNNNNNTGG 1 cut(s) 28
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.