Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0016312

Physical Location & Seq

Genomic Coordinates Forward (+)

10122234 .. 10127037

Gene Structure 4804 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1133000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3087 bp

ATGAATAAAATGGATTTCAATATCGGAATCGAGGATGTAGGAGTGGCAGTGCTGCAGGAATTATGGAATAAGGTTGCAATTCAAGCCACAGGGCTTGTTAGCGAAACGAAGGATCTGTTGTTTGAAAAGGACAGCTTTCTGGAGTTCTCCAGGAGTATATCTGAGCTAAATATTCTCCTCGACAAGTTGAATGCCCGGAAGGTTGAAAACGCGCTGGGTTTAGAGTCCACAAAGGCTAAACTGACCACATTGAACCGTCAGTTGAAAAAAGCTAGCAAGATAATCAAGGATTACAAATATGGAAGCCGCCTCCGTCTCTTGCTTAAATCCCACTCAATGCTCCTGCAGATGCAGGAAGTAGCTAAAGATATTGCTAATACCATCTCCTCATTTCAGCTGGTCAATCTTGATATCGCGCTGCACTTGAATTCGATGACTAACCAGATCATCAACAATCTGGAGTCAATGGAGTTCAGGTCAGCGGCTGCAACAGAATCAATTGCTTCAGAGATGGAGAATTCGATGTCACAAAATGCTACGAATAGGGAGAATGCTCAAAAGCTTTTGGAAAAGATTGCAGATGCCGTTGGTGCTAGGGCAAATTCATCCTTGGTACAAAATGAGCTGCAACTTTTGAAGCAAGAGAAAGAAGAGATGGAAGCACAAAAGAAGCAGGCAGAGGCACTCCAGCTATCGCAACTGATTGACTTCCTCTATGCTACAGAAGTTGTTACTAGACCAGATGACGAGGGAACCTCTACTTACCACCAGCAGTACCCAATTGGTTCGTTCATATGCGAACTTTGCAAAAAGATGATGACAGACCCCGTAGCAATTATCTGCGGTCACAGCTTTGAGAGGAAGGCAATTCAGGAGCACTTTAGACGAGGAGAGAAGAACTGCCCCACCTGCAGACAGGAGCTTTCATCTCTAGAGCTCACACCTAACCTTTTGCTTCGAAACTCTATAGAAGAATGGAATCAAAGAGACCAGGACCTGAAATTCCAAGCTGCAGTTCATGGACTCAAATCCATTGACAACTCGAGACAGGACACGGCTTTAGAGGACATGCAAGTTCTTTTAAAAATGCCAAGATATGTAACCAAAGCTGCAGAGGAAGGACTTGCAACAAAGCTGGCGGTAATCTTAAAAGATGAAGGGGTAAATGCTGTGGCAGCATTAAAATGTCTCTACTACTTAGCTAAACACAACAATGAACAAAAGGAAACCATTGTCAAAGCAGGGGGCATTCGGAGAATTGTGAAGCATATCTACAAAGCCGGAAGCAAACATGATGCCGTTGCAGTCTTGTTGGAGTTGTCAGCAAAGGAAACCCTTGGGGAGAAAATAGGCAATGCAAAAGATTGCATTCCTCTTCTGGTTTCTTTACTCCATAAAGATAACACTGAAGTGTCACAGAAATCTCGTAAGGTATTGCAGAACCTCTCCTTGAACACACATTTTGTTGTCAAGATGGCTGAAGCAGGACACTTTCAACCTTTCGTTGCTCGCTTCAATGAAGCACCACAGGAGACCAGAACATTGATGGCTGCAGCCTTGGTGAATATGCAACTCAAGGAGAATAGTGTTGAAGACTTAAAGGAGCCGCAATTTATACAGAATTTGGTTCAGATGCTGTCTTCAAGCTCTCCAGCATGCAAATCAGCTTGCCTTAAATCCATCAAGAAACTCGTAGCATATCCCAGGATCGTAAAACGACTTCTTAAAGACCCTGCCACTATACCACATTTGCTTGGTCTCATCTCATTTAACAAGTCTGATCCACACTTGAAACAAGAAGCAGCTGAGATTCTTGCTAACATGATTGGAGCGAGTCAACAATTTGAACAACTAAAATATCAGGGCTTGGAGGAGCTGCAATCGAAGTACAATGTCTGTGTACTATTGCATCTTGTAACCAGCTCCGAAGATCAAACCAAGATTCAATTCTTGCACCTACTGGTTGAACTAAGCTATAAATCAGAGATAGCTCGAGATATAATACGATCTGAACAGGAGGCAACAGCTCACCTTTTCTCTTCCCTTTATAGCGATCATCCTGCTGTGAGAAGATGGGCAATGAAACTTATATATTGCATATCCAAAGATCATCCAGATGGGGTTCCCCTTCCACCATCTCCTGCAAAAGAAAGTGCCATTAACACCCTGTCAATGATCTTCATTAATTCACCGGATATTGAAGAAAGGTCAACTGCGGCAGGAATTATCAGCCAGCTTCCCAGGGAAGATAGTAGTATTGATGAGATACTCCGAAAATCAGAAGTACTAAAAGCCATTCATGAGGTAATTTGCAGCATGGATGAGGAAAATTGGGGGAGCAGAGCACAATCCAACCAAGGCACATCTCTACTGGAGAATGCTCTAGCAGCCCTTTTGCGCTACACAGAACCCAACAAACCCGAACTTCAGAAACAATTGGGCAGGCTTGAAGTGTACCCATCACTAGTTCGTGTTCTAACCCGTGGCAGCTCACTAGCTAAGCAGAGAACAGCCATTGCACTTGCGCAACTTTCCCAGTCTGCCAGCCTGTCAGTCTCCAATGAAACCATCAAGCAAACGAAGCACCCTGTGCCCCTCTTTGATTTAATGAAGAATGTTTCCATTATGTTGTGCTTTTCGGCATCATCAGAGAATGCAAACTCCTGTCCTGTTCATGGTGCTGCTTGTTCACCAAGAGATACCTTCTGCCTGGTTAAGGCAGATGCTGTGAAGCCGCTTGTGCGCACTTTGAGTGAGACGGAGTCTGGTGTGGCAGAGGCAGCTCTGACGGCACTTGAAACATTGCTAATTGACCACAGCACACTAACAGATGCAACTGCTGCCATTGTGGACAACCAGGGTGTGGTTGCCATTCTGCAACTCTTGGACAAAGGATCCTCATCTGCAAAAACCAAAGCCTTAGACCTGTTTCAGAAGATCTTGGTACATACAACTATCGCTGAGACGTTAAAGAAGAGGTTTGAGAATATCCTTGTCCAACTTCTCCAGGATGATGAACTAAAGAAAAAAGCAGCTTTGGTACTTAGGCAGATGGGAATCATCCCCGATCAGTCCTCATACTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006807 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009889 GO:0009890 GO:0009892 GO:0009894 GO:0009987 GO:0010115 GO:0010150 GO:0010271 GO:0010380 GO:0010565 GO:0016020 GO:0016567 GO:0016740 GO:0019216 GO:0019222 GO:0019538 GO:0019747 GO:0019787 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031329 GO:0032446 GO:0032501 GO:0032502 GO:0033612 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043455 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045827 GO:0045833 GO:0046890 GO:0048366 GO:0048367 GO:0048519 GO:0048523 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0051055 GO:0051171 GO:0051193 GO:0062012 GO:0062014 GO:0065007 GO:0070647 GO:0070696 GO:0071704 GO:0071944 GO:0080090 GO:0090056 GO:0090359 GO:0090693 GO:0099402 GO:0140096 GO:1901401 GO:1901404 GO:1901463 GO:1901564 GO:1902930 GO:1902931

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

U-box

Protein Analysis

1029

Amino Acids

114.38

Weight (kDa)

6.81

Isoelectric Point (pI)

45.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
U-box	PF04564	264 - 329	3.1e-13	U-box domain
ARM_ARMC5	PF24768	374 - 512	1.2e-06	ARMC5

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0016312)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g22300 FvH4_4g22300
malus_domestica	MD13G1133000.v1.1
prunus_persica	Prupe.1G212500_v2.0.a1
pyrus_communis	pycom13g11540
rosa_chinensis	RchiOBHm_Chr4g0428541
rosa_laevigata	RLG00000007144
rosa_multiflora	Rmu_sc0002072.1_g000017
rosa_roxburghii	Rroxscaffold_5G00370240
rosa_rugosa	Rorug04G0226100
rosa_samantha	Rh4AG283600 Rh4BG289300 Rh4CG305200 Rh4DG286300
rosa_wichuraiana	Rw4G024620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	917
Acc16I	TGCGCA	2 cut(s)	2526, 2744
Acc36I	ACCTGC	1 cut(s)	917
AccB7I	CCANNNNNTGG	1 cut(s)	2863
AccII	CGCG	2 cut(s)	212, 416
AciI	CCGC	7 cut(s)	307, 482, 843, 1139, 1607, 2216, 2735
AclWI	GGATC	5 cut(s)	120, 1715, 1775, 2889, 2902
AcsI	RAATTY	5 cut(s)	427, 517, 601, 1001, 1621
AcuI	CTGAAG	4 cut(s)	489, 1428, 1500, 2411
AdeI	CACNNNGTG	1 cut(s)	2590
AfaI	GTAC	8 cut(s)	615, 776, 1889, 1902, 2286, 2456, 2946, 3042
AfiI	CCNNNNNNNGG	3 cut(s)	2675, 2716, 2863
AhlI	ACTAGT	1 cut(s)	2464
AjnI	CCWGG	7 cut(s)	149, 990, 1703, 2240, 2709, 2856, 3006
AjuI	GAANNNNNNNTTGG	4 cut(s)	999, 1031, 1931, 1963
AleI	CACNNNNGTG	1 cut(s)	1409
Alw21I	GWGCWC	3 cut(s)	879, 939, 2347
Alw26I	GTCTC	9 cut(s)	320, 981, 1039, 1193, 1526, 1763, 2560, 2750, 2957
AlwI	GGATC	5 cut(s)	120, 1715, 1775, 2889, 2902
AlwNI	CAGNNNCTG	4 cut(s)	485, 997, 1636, 2726
Ama87I	CYCGRG	2 cut(s)	1042, 1992
ApoI	RAATTY	5 cut(s)	427, 517, 601, 1001, 1621
AseI	ATTAAT	1 cut(s)	2184
AspLEI	GCGC	5 cut(s)	214, 418, 2400, 2527, 2745
AspS9I	GGNCC	1 cut(s)	994
AsuC2I	CCSGG	1 cut(s)	196
AsuHPI	GGTGA	4 cut(s)	1573, 2021, 2181, 2682
AsuII	TTCGAA	1 cut(s)	958
AsuNHI	GCTAGC	1 cut(s)	272
AvaI	CYCGRG	2 cut(s)	1042, 1992
AvaII	GGWCC	1 cut(s)	994
BaeGI	GKGCMC	1 cut(s)	2595
BamHI	GGATCC	1 cut(s)	2894
BanII	GRGCYC	1 cut(s)	939
BarI	GAAGNNNNNNTAC	4 cut(s)	1256, 1288, 1704, 1736
BbsI	GAAGAC	2 cut(s)	1599, 1632
Bbv12I	GWGCWC	3 cut(s)	879, 939, 2347
BceAI	ACGGC	4 cut(s)	569, 1071, 1283, 2805
BcgI	CGANNNNNNTGC	2 cut(s)	2042, 2076
BciT130I	CCWGG	7 cut(s)	151, 992, 1705, 2242, 2711, 2858, 3008
BcnI	CCSGG	1 cut(s)	196
BcoDI	GTCTC	9 cut(s)	320, 981, 1039, 1193, 1526, 1763, 2560, 2750, 2957
BcuI	ACTAGT	1 cut(s)	2464
BfaI	CTAG	7 cut(s)	273, 594, 735, 932, 2384, 2465, 2495
BfmI	CTRYAG	8 cut(s)	53, 344, 720, 910, 966, 1011, 1110, 1551
BfuAI	ACCTGC	1 cut(s)	917
BglII	AGATCT	1 cut(s)	2937
BlpI	GCTNAGC	1 cut(s)	2499
BmcAI	AGTACT	1 cut(s)	2286
Bme1390I	CCNGG	8 cut(s)	151, 196, 992, 1705, 2242, 2711, 2858, 3008
Bme18I	GGWCC	1 cut(s)	994
BmeT110I	CYCGRG	2 cut(s)	1042, 1992
BmgT120I	GGNCC	1 cut(s)	994
BmiI	GGNNCC	4 cut(s)	754, 1605, 2124, 2896
BmrFI	CCNGG	8 cut(s)	151, 196, 992, 1705, 2242, 2711, 2858, 3008
BmrI	ACTGGG	1 cut(s)	2530
BmsI	GCATC	8 cut(s)	339, 571, 1285, 1623, 1918, 2651, 2713, 2821
BmtI	GCTAGC	1 cut(s)	276
BmuI	ACTGGG	1 cut(s)	2530
BpiI	GAAGAC	2 cut(s)	1599, 1632
BplI	GAGNNNNNCTC	2 cut(s)	740, 772
BpmI	CTGGAG	7 cut(s)	133, 161, 479, 671, 1635, 2393, 2990
Bpu1102I	GCTNAGC	1 cut(s)	2499
Bpu14I	TTCGAA	1 cut(s)	958
BpuEI	CTTGAG	1 cut(s)	1559
BpuMI	CCSGG	1 cut(s)	196
BsaBI	GATNNNNATC	2 cut(s)	2899, 3056
BsaI	GGTCTC	3 cut(s)	981, 1526, 1763
BsaJI	CCNNGG	9 cut(s)	609, 1336, 1557, 1703, 2240, 2241, 2356, 2482, 2857
BsaWI	WCCGGW	1 cut(s)	2191
BsaXI	ACNNNNNCTCC	4 cut(s)	33, 63, 461, 491
Bsc4I	CCNNNNNNNGG	3 cut(s)	2675, 2716, 2863
Bse1I	ACTGG	3 cut(s)	1965, 2376, 2536
Bse3DI	GCAATG	4 cut(s)	1360, 2085, 2514, 2801
Bse8I	GATNNNNATC	2 cut(s)	2899, 3056
BseBI	CCWGG	7 cut(s)	151, 992, 1705, 2242, 2711, 2858, 3008
BseDI	CCNNGG	9 cut(s)	609, 1336, 1557, 1703, 2240, 2241, 2356, 2482, 2857
BseGI	GGATG	7 cut(s)	40, 605, 2056, 2110, 2326, 3016, 3060
BseJI	GATNNNNATC	2 cut(s)	2899, 3056
BseLI	CCNNNNNNNGG	3 cut(s)	2675, 2716, 2863
BseMI	GCAATG	4 cut(s)	1360, 2085, 2514, 2801
BseMII	CTCAG	3 cut(s)	153, 1797, 2952
BseNI	ACTGG	3 cut(s)	1965, 2376, 2536
BseRI	GAGGAG	4 cut(s)	167, 376, 903, 1886
BseSI	GKGCMC	1 cut(s)	2595
BseYI	CCCAGC	1 cut(s)	214
BsgI	GTGCAG	1 cut(s)	404
Bsh1236I	CGCG	2 cut(s)	212, 416
BsiHKAI	GWGCWC	3 cut(s)	879, 939, 2347
BsiHKCI	CYCGRG	2 cut(s)	1042, 1992
BsiSI	CCGG	3 cut(s)	196, 1281, 2192
BslI	CCNNNNNNNGG	3 cut(s)	2675, 2716, 2863
BsmAI	GTCTC	9 cut(s)	320, 981, 1039, 1193, 1526, 1763, 2560, 2750, 2957
BsmBI	CGTCTC	3 cut(s)	320, 2750, 2957
BsmI	GAATGC	6 cut(s)	196, 556, 1248, 1368, 2383, 2659
Bso31I	GGTCTC	3 cut(s)	981, 1526, 1763
BsoBI	CYCGRG	2 cut(s)	1042, 1992
Bsp119I	TTCGAA	1 cut(s)	958
Bsp1286I	GDGCHC	4 cut(s)	879, 939, 2347, 2595
Bsp1720I	GCTNAGC	1 cut(s)	2499
BspACI	CCGC	7 cut(s)	307, 482, 843, 1139, 1607, 2216, 2735
BspCNI	CTCAG	3 cut(s)	154, 1798, 2953
BspFNI	CGCG	2 cut(s)	212, 416
BspHI	TCATGA	1 cut(s)	2299
BspLI	GGNNCC	4 cut(s)	754, 1605, 2124, 2896
BspMAI	CTGCAG	6 cut(s)	57, 348, 914, 1015, 1114, 1555
BspMI	ACCTGC	1 cut(s)	917
BspOI	GCTAGC	1 cut(s)	276
BspPI	GGATC	5 cut(s)	120, 1715, 1775, 2889, 2902
BspT104I	TTCGAA	1 cut(s)	958
BspTNI	GGTCTC	3 cut(s)	981, 1526, 1763
BsrDI	GCAATG	4 cut(s)	1360, 2085, 2514, 2801
BsrI	ACTGG	3 cut(s)	1965, 2376, 2536
BssECI	CCNNGG	9 cut(s)	609, 1336, 1557, 1703, 2240, 2241, 2356, 2482, 2857
BssT1I	CCWWGG	4 cut(s)	609, 1336, 1557, 2356
Bst2UI	CCWGG	7 cut(s)	151, 992, 1705, 2242, 2711, 2858, 3008
Bst4CI	ACNGT	1 cut(s)	257
Bst6I	CTCTTC	4 cut(s)	645, 1380, 2044, 2969
BstAPI	GCANNNNNTGC	2 cut(s)	2590, 2840
BstBI	TTCGAA	1 cut(s)	958
BstC8I	GCNNGC	9 cut(s)	274, 675, 1137, 1510, 1657, 1669, 2234, 2444, 2545
BstDEI	CTNAG	8 cut(s)	162, 1198, 1806, 1970, 2499, 2920, 2961, 3044
BstDSI	CCRYGG	1 cut(s)	2482
BstENI	CCTNNNNNAGG	1 cut(s)	2714
BstF5I	GGATG	7 cut(s)	40, 605, 2056, 2110, 2326, 3016, 3060
BstFNI	CGCG	2 cut(s)	212, 416
BstHHI	GCGC	5 cut(s)	214, 418, 2400, 2527, 2745
BstMAI	GTCTC	9 cut(s)	320, 981, 1039, 1193, 1526, 1763, 2560, 2750, 2957
BstNI	CCWGG	7 cut(s)	151, 992, 1705, 2242, 2711, 2858, 3008
BstNSI	RCATGY	2 cut(s)	1072, 1659
BstSCI	CCNGG	8 cut(s)	149, 194, 990, 1703, 2240, 2709, 2856, 3006
BstSFI	CTRYAG	8 cut(s)	53, 344, 720, 910, 966, 1011, 1110, 1551
BstSLI	GKGCMC	1 cut(s)	2595
BstUI	CGCG	2 cut(s)	212, 416
BstV2I	GAAGAC	2 cut(s)	1599, 1632
BstX2I	RGATCY	3 cut(s)	112, 2894, 2937
BstYI	RGATCY	3 cut(s)	112, 2894, 2937
BtgI	CCRYGG	1 cut(s)	2482
BtsCI	GGATG	7 cut(s)	40, 605, 2056, 2110, 2326, 3016, 3060
BtsI	GCAGTG	1 cut(s)	54
BtsIMutI	CAGTG	2 cut(s)	54, 1404
BveI	ACCTGC	1 cut(s)	917
Cac8I	GCNNGC	9 cut(s)	274, 675, 1137, 1510, 1657, 1669, 2234, 2444, 2545
CaiI	CAGNNNCTG	4 cut(s)	485, 997, 1636, 2726
CciI	TCATGA	1 cut(s)	2299
CfoI	GCGC	5 cut(s)	214, 418, 2400, 2527, 2745
Cfr13I	GGNCC	1 cut(s)	994
Csp6I	GTAC	8 cut(s)	614, 775, 1888, 1901, 2285, 2455, 2945, 3041
CspCI	CAANNNNNGTGG	2 cut(s)	76, 111
CviAII	CATG	8 cut(s)	1019, 1069, 1292, 1656, 1822, 2300, 2317, 2675
CviQI	GTAC	8 cut(s)	614, 775, 1888, 1901, 2285, 2455, 2945, 3041
DdeI	CTNAG	8 cut(s)	162, 1198, 1806, 1970, 2499, 2920, 2961, 3044
DraI	TTTAAA	1 cut(s)	1083
DraIII	CACNNNGTG	1 cut(s)	2590
Eam1104I	CTCTTC	4 cut(s)	645, 1380, 2044, 2969
EarI	CTCTTC	4 cut(s)	645, 1380, 2044, 2969
Ecl136II	GAGCTC	1 cut(s)	937
Eco130I	CCWWGG	4 cut(s)	609, 1336, 1557, 2356
Eco24I	GRGCYC	1 cut(s)	939
Eco31I	GGTCTC	3 cut(s)	981, 1526, 1763
Eco32I	GATATC	1 cut(s)	412
Eco47I	GGWCC	1 cut(s)	994
Eco53kI	GAGCTC	1 cut(s)	937
Eco57I	CTGAAG	4 cut(s)	489, 1428, 1500, 2411
Eco88I	CYCGRG	2 cut(s)	1042, 1992
EcoICRI	GAGCTC	1 cut(s)	937
EcoNI	CCTNNNNNAGG	1 cut(s)	2714
EcoO109I	RGGNCCY	1 cut(s)	994
EcoRI	GAATTC	2 cut(s)	427, 517
EcoRII	CCWGG	7 cut(s)	149, 990, 1703, 2240, 2709, 2856, 3006
EcoRV	GATATC	1 cut(s)	412
EcoT14I	CCWWGG	4 cut(s)	609, 1336, 1557, 2356
EcoT38I	GRGCYC	1 cut(s)	939
ErhI	CCWWGG	4 cut(s)	609, 1336, 1557, 2356
Esp3I	CGTCTC	3 cut(s)	320, 2750, 2957
FaeI	CATG	8 cut(s)	1022, 1072, 1295, 1659, 1825, 2303, 2320, 2678
FalI	AAGNNNNNCTT	2 cut(s)	119, 151
FatI	CATG	8 cut(s)	1018, 1068, 1291, 1655, 1821, 2299, 2316, 2674
FauNDI	CATATG	1 cut(s)	794
FokI	GGATG	7 cut(s)	47, 592, 2043, 2097, 2333, 3023, 3047
FriOI	GRGCYC	1 cut(s)	939
FspAI	RTGCGCAY	1 cut(s)	2744
FspBI	CTAG	7 cut(s)	273, 594, 735, 932, 2384, 2465, 2495
FspI	TGCGCA	2 cut(s)	2526, 2744
GlaI	GCGC	5 cut(s)	213, 417, 2399, 2526, 2744
GsaI	CCCAGC	1 cut(s)	218
GsuI	CTGGAG	7 cut(s)	133, 161, 479, 671, 1635, 2393, 2990
HapII	CCGG	3 cut(s)	196, 1281, 2192
HhaI	GCGC	5 cut(s)	214, 418, 2400, 2527, 2745
Hin1II	CATG	8 cut(s)	1022, 1072, 1295, 1659, 1825, 2303, 2320, 2678
Hin6I	GCGC	5 cut(s)	212, 416, 2398, 2525, 2743
HinP1I	GCGC	5 cut(s)	212, 416, 2398, 2525, 2743
HincII	GTYRAC	2 cut(s)	1838, 2211
HindII	GTYRAC	2 cut(s)	1838, 2211
HindIII	AAGCTT	1 cut(s)	560
HpaII	CCGG	3 cut(s)	196, 1281, 2192
HphI	GGTGA	4 cut(s)	1573, 2021, 2181, 2682
Hpy166II	GTNNAC	7 cut(s)	228, 1838, 1901, 2211, 2455, 2690, 2851
Hpy8I	GTNNAC	7 cut(s)	228, 1838, 1901, 2211, 2455, 2690, 2851
HpyAV	CCTTC	7 cut(s)	103, 193, 856, 1112, 1151, 2138, 2713
HpyCH4III	ACNGT	1 cut(s)	257
HpyCH4IV	ACGT	1 cut(s)	2966
HpyF3I	CTNAG	8 cut(s)	162, 1198, 1806, 1970, 2499, 2920, 2961, 3044
HpySE526I	ACGT	1 cut(s)	2966
Hsp92II	CATG	8 cut(s)	1022, 1072, 1295, 1659, 1825, 2303, 2320, 2678
HspAI	GCGC	5 cut(s)	212, 416, 2398, 2525, 2743
LmnI	GCTCC	8 cut(s)	345, 874, 919, 1603, 1829, 1873, 1928, 2337
LweI	GCATC	8 cut(s)	339, 571, 1285, 1623, 1918, 2651, 2713, 2821
MaeI	CTAG	7 cut(s)	273, 594, 735, 932, 2384, 2465, 2495
MaeII	ACGT	1 cut(s)	2966
MaeIII	GTNAC	6 cut(s)	525, 730, 845, 1099, 1413, 1915
MfeI	CAATTG	3 cut(s)	498, 780, 2435
MflI	RGATCY	3 cut(s)	112, 2894, 2937
MhlI	GDGCHC	4 cut(s)	879, 939, 2347, 2595
MlyI	GAGTC	5 cut(s)	233, 470, 1017, 1843, 2771
MmeI	TCCRAC	3 cut(s)	1293, 2376, 3022
MslI	CAYNNNNRTG	4 cut(s)	1183, 1212, 1409, 2115
MspA1I	CMGCKG	3 cut(s)	397, 482, 1805
MspI	CCGG	3 cut(s)	196, 1281, 2192
MspR9I	CCNGG	8 cut(s)	151, 196, 992, 1705, 2242, 2711, 2858, 3008
MunI	CAATTG	3 cut(s)	498, 780, 2435
Mva1269I	GAATGC	6 cut(s)	196, 556, 1248, 1368, 2383, 2659
MvaI	CCWGG	7 cut(s)	151, 992, 1705, 2242, 2711, 2858, 3008
MvnI	CGCG	2 cut(s)	212, 416
NciI	CCSGG	1 cut(s)	196
NdeI	CATATG	1 cut(s)	794
NheI	GCTAGC	1 cut(s)	272
NlaIII	CATG	8 cut(s)	1022, 1072, 1295, 1659, 1825, 2303, 2320, 2678
NlaIV	GGNNCC	4 cut(s)	754, 1605, 2124, 2896
NmuCI	GTSAC	3 cut(s)	525, 845, 1413
NsbI	TGCGCA	2 cut(s)	2526, 2744
NspI	RCATGY	2 cut(s)	1072, 1659
NspV	TTCGAA	1 cut(s)	958
OliI	CACNNNNGTG	1 cut(s)	1409
PaeI	GCATGC	1 cut(s)	1659
PaeR7I	CTCGAG	2 cut(s)	1042, 1992
PagI	TCATGA	1 cut(s)	2299
PaqCI	CACCTGC	1 cut(s)	917
PasI	CCCWGGG	1 cut(s)	2241
PctI	GAATGC	6 cut(s)	196, 556, 1248, 1368, 2383, 2659
PfeI	GAWTC	6 cut(s)	27, 494, 979, 1810, 1942, 3057
PflFI	GACNNNGTC	1 cut(s)	2761
PflMI	CCANNNNNTGG	1 cut(s)	2863
PfoI	TCCNGGA	2 cut(s)	149, 3006
PleI	GAGTC	5 cut(s)	232, 469, 1017, 1842, 2770
PpsI	GAGTC	5 cut(s)	232, 469, 1017, 1842, 2770
PpuMI	RGGWCCY	1 cut(s)	994
PshBI	ATTAAT	1 cut(s)	2184
Psp124BI	GAGCTC	1 cut(s)	939
Psp5II	RGGWCCY	1 cut(s)	994
Psp6I	CCWGG	7 cut(s)	149, 990, 1703, 2240, 2709, 2856, 3006
PspFI	CCCAGC	1 cut(s)	214
PspGI	CCWGG	7 cut(s)	149, 990, 1703, 2240, 2709, 2856, 3006
PspN4I	GGNNCC	4 cut(s)	754, 1605, 2124, 2896
PspPI	GGNCC	1 cut(s)	994
PspPPI	RGGWCCY	1 cut(s)	994
PstI	CTGCAG	6 cut(s)	57, 348, 914, 1015, 1114, 1555
PstNI	CAGNNNCTG	4 cut(s)	485, 997, 1636, 2726
PsuI	RGATCY	3 cut(s)	112, 2894, 2937
PsyI	GACNNNGTC	1 cut(s)	2761
PvuII	CAGCTG	2 cut(s)	397, 1805
RsaI	GTAC	8 cut(s)	615, 776, 1889, 1902, 2286, 2456, 2946, 3042
RsaNI	GTAC	8 cut(s)	614, 775, 1888, 1901, 2285, 2455, 2945, 3041
RseI	CAYNNNNRTG	4 cut(s)	1183, 1212, 1409, 2115
SacI	GAGCTC	1 cut(s)	939
Sau96I	GGNCC	1 cut(s)	994
ScaI	AGTACT	1 cut(s)	2286
SchI	GAGTC	5 cut(s)	233, 470, 1017, 1843, 2771
ScrFI	CCNGG	8 cut(s)	151, 196, 992, 1705, 2242, 2711, 2858, 3008
SduI	GDGCHC	4 cut(s)	879, 939, 2347, 2595
SfaNI	GCATC	8 cut(s)	339, 571, 1285, 1623, 1918, 2651, 2713, 2821
SfcI	CTRYAG	8 cut(s)	53, 344, 720, 910, 966, 1011, 1110, 1551
Sfr274I	CTCGAG	2 cut(s)	1042, 1992
SfuI	TTCGAA	1 cut(s)	958
SinI	GGWCC	1 cut(s)	994
SlaI	CTCGAG	2 cut(s)	1042, 1992
SmiMI	CAYNNNNRTG	4 cut(s)	1183, 1212, 1409, 2115
SmlI	CTYRAG	3 cut(s)	1042, 1574, 1992
SmoI	CTYRAG	3 cut(s)	1042, 1574, 1992
SpeI	ACTAGT	1 cut(s)	2464
SphI	GCATGC	1 cut(s)	1659
SsiI	CCGC	7 cut(s)	307, 482, 843, 1139, 1607, 2216, 2735
SspI	AATATT	1 cut(s)	172
SspMI	CTAG	7 cut(s)	273, 594, 735, 932, 2384, 2465, 2495
SstI	GAGCTC	1 cut(s)	939
StyD4I	CCNGG	8 cut(s)	149, 194, 990, 1703, 2240, 2709, 2856, 3006
StyI	CCWWGG	4 cut(s)	609, 1336, 1557, 2356
TaaI	ACNGT	1 cut(s)	257
TaiI	ACGT	1 cut(s)	2969
TaqI	TCGA	8 cut(s)	30, 180, 431, 521, 958, 1043, 1883, 1993
TatI	WGTACW	3 cut(s)	1887, 1900, 2284
TauI	GCSGC	5 cut(s)	309, 485, 1609, 2219, 2737
TfiI	GAWTC	6 cut(s)	27, 494, 979, 1810, 1942, 3057
TscAI	CASTG	2 cut(s)	54, 1411
TseFI	GTSAC	3 cut(s)	525, 845, 1413
Tsp45I	GTSAC	3 cut(s)	525, 845, 1413
TspGWI	ACGGA	2 cut(s)	302, 2774
TspRI	CASTG	2 cut(s)	54, 1411
Tth111I	GACNNNGTC	1 cut(s)	2761
Van91I	CCANNNNNTGG	1 cut(s)	2863
VpaK11BI	GGWCC	1 cut(s)	994
VspI	ATTAAT	1 cut(s)	2184
XagI	CCTNNNNNAGG	1 cut(s)	2714
XapI	RAATTY	5 cut(s)	427, 517, 601, 1001, 1621
XbaI	TCTAGA	1 cut(s)	931
XceI	RCATGY	2 cut(s)	1072, 1659
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1543
XhoI	CTCGAG	2 cut(s)	1042, 1992
XspI	CTAG	7 cut(s)	273, 594, 735, 932, 2384, 2465, 2495
ZrmI	AGTACT	1 cut(s)	2286

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.