Basic Information

Type: gene

Biological Identity

Species

prunus_persica

Chromosome

Pp01

Orthogroup ID

OG0016312

Physical Location & Seq

Genomic Coordinates Reverse (-)

22589433 .. 22593229

Gene Structure 3797 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Prupe.1G212500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3078 bp

ATGGCCACAATGGACTTCAATATCGGAATTGAGGATGTAGGAGTGGCAGTACTACAGGAATTATGGAATAAGGTGGGATTTCAGGCCACGGGGCTCGTGGGCGAAACGAAGGATCTATTGTTTGAAAAGGACAGCTTTCTGGAGTTCTCCAGGAGTATTTCTGAGCTCAATATCCTCCTCAGTAGCTTGAATGCCCGAAAAGTTGAAAATGCACTGGGATTAGAGTCCACAAAGGCTGCACTGACAACATTGAGCATACAGTTGAAGAAAGCAAGCAAGATCATCAAGGATTACAAATGTGGAAGCCGCCTCCGCCTCTTGCTTAAATCACACTCAATGCTCTTGCAGATGGAGGATGTAGCTAAAGATATTGCCAAGACTGTCTCCTCTTTTCAGCTGATCAATCTTGATATCTCCCTGAGCTTGAATACTATGACCAAACAGATCATCAACAATCTAGGATCAATGGAATTCAGGTCAGCATGTGCAACGGAATCAATTGCTTCAGAGATGGAGAATTCAATTTCTCAAAATGCTAGGAATAGGGAGAATTCTCAAAAGCTTCTGGAGAAGGTTGCAGAAGCCGTTGGTGCTCGAGCGAATGCGTTCTTGGTACAAAATGAGTTGGCACTCTTGAAGCAAGAGAAAGAAGACATGGAAGCTCAAAAGAAGCAGGCGGAGGCACTTCAGCTATCTCAACTGATTGACTTCCTCTACAGTACAGAAATTGTTACCAGACCAAATGATGAGGAAACCTCTACATACCACCAGCAGTATCCAATTGATTCATTCATGTGTGAACTTTGCAAAAAGATGATGGAAGACCCAGTAGCAGTTACATGTGGCCACAGCTTTGAGAGGAAGGCAATTCAGGAACACTTTGGGCGTGGAGAGAGGAACTGCCCCATCTGCAGACAGGAGCTTTCATCACTAGAGCTCACGCCTAATGTTGTGCTGCGAAACTCTATTGAAGAATGGAATCAGAGAGACAAGGATTTAAAATTCCAAGCTGCAGTCCATGGAGTCAAATCCAGTGACCGCTCTAAAATGGACAAGGCTTTAGAGGACATGCAGTTTCTTTTGGAAATGCCTAGATATGCAACCAAAGCTGCAGAGGAAGGACTTGCAACAAAGTTGGTAGTGATCTTAAAAGACGATACAGTAAATTCTGTGGCAGTATTAAAATGTCTCTACTACCTAGCTAAACTTAATGAGGATCAAAAGGAAGCCATTGTCAGAGCAGGGGCCATTCGAAGAATTGTGAAGTATATCTACAAAGGAGGAAGTAAAAGGGATGCCATTGCAGTCTTGTTGGAGTTGTCAGCAAAGGAAACCATTGGGCAGAAAATAGGAGATACAAAAGATTGTATTCCTCTTCTGGTTTCTTTACTCCATAAGAATAACCCTGATGTGTCACAGGAAGCTTGTAAGGTGTTGCAGAACCTCTCGTCAAACACACATTTTGTTGTCAAGATGGCTGAAGCAGGACACTTCCAACCTTTTGTTGCTCGCTTCAATGAAGCACCTCAGGAGACCAGAACATTGATGGCTGCAGCCTTGATAAAGATGCAACTCAAGGAGAATAGCGTTGAAGAATTAAAGGACTGGCAATTTATACAGAGTTTACTTCAGATGCTCTCTTCGAGCTCACCAGCATGCAAATCTGCTTGCCTAAAATGTATGAAGAAACTTGTAGCACACCACAAGATTGTAAAGCGACTTCTGAAAGACCCTGCCACTGTACCACATTTGCTTGGCCTCATCTCATTTAACATGTCTGATCCACACTTGAAACAAGAAGCTGCTGAGATTCTTGCTAACATGATTGGAGCCAGCAAACAATTTGAACAACAAAAGTATCAGGGCTTGCAAGAGCTCCAATCAAAGCACAATGTCTGTCTACTGTTGCAGCTTGTAACCAGTGCTGAAGATCAAACCAAGATTCAATTCTTGCACCTACTGGTTGCACTGAGCTATAAATCAGAGATAGCTCGAGATATAATACGATCTGAACAGGATGCAATTGCTCACCTCTTCTCTTCCCTTCACAGTGATCATCGTGTGGTGAAAAGATGGGCAATGAAGCTCATATATTGCATATCAGAAGGTCATACTGCTGGGGTTCCACTACCACCTTCCCCTGCAAAAGAAACTGCTATTAACACCCTGGCAACCATCCTCATTAATTCACCAGACATTGAAGAAAGGTCCACTGTTGCAGGAATTATCAGCCAGCTTCCGAGGGATGATAGTAGTATTGATGAGATACTCCGCAAATCAGAAGTACTAAAAGCCATTCATGAGGTAATTTGCAGCATGGATGAGGAAAATTGGGGGAACATAGCACCTTCCATCCAAGGCACATCTCTACTGGAGAATGCTCTAGCAGCCCTTCTGCGCTACACAGAACCCACCAAGCCTGAACTTCAGAGACAATTGGGCAAGCTTGAAGTGTACCCATCATTAGTTCGTGTCTTAACCAGGGGTAGCTCACTAGCTAAGCAGAGAACAGCCATTGCACTTGCCCAACTATCCCAGTCTACTAGTCTGTCAGTCTCCGAAGAAACCATCAGGCAAACCAAGCCCTCGACGCCCCTCTTTGATCTGATGAAGCTTTTCTTGTGCTTTTCAGCATCATCAGAGAATGGAAGTATATGTTCTGTTCATGGTGCTGCTTGTTCACCAAGAGATACCTTCTGCCTGGTCAAGGCAGACGCGGTGAGGCCATTGGTGCGGACTTTGAGTAACACAGAATCTGGTGTGGCAGAGGCTGCTCTGATGGCACTTGAAACATTGCTGACAGACCACAGCACACTTTCACATGCAACTGCTGCCATTGTGGACAACCAGGGTGTGGTGGCCATTCTGCAAGTCTTGGACAGAGGATCTTTATCTGCCAAAACCAAAGCCTTAGACCTCTTTCAGAAGATCCTGGTTCATACAACGATCAGTGATACATTAAAGCAGAGGTTCGAGAGGATCCTTATCCAACTCCTCCATGATGATGAGCTTAAGAAAAAAGCAGCTTTGGTGCTTAGGCAGATGGAAATCATCCCGGAGCAGTCCTCATACTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006807 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009889 GO:0009890 GO:0009892 GO:0009894 GO:0009987 GO:0010115 GO:0010150 GO:0010271 GO:0010380 GO:0010565 GO:0016020 GO:0016567 GO:0016740 GO:0019216 GO:0019222 GO:0019538 GO:0019747 GO:0019787 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031329 GO:0032446 GO:0032501 GO:0032502 GO:0033612 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043455 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045827 GO:0045833 GO:0046890 GO:0048366 GO:0048367 GO:0048519 GO:0048523 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0051055 GO:0051171 GO:0051193 GO:0062012 GO:0062014 GO:0065007 GO:0070647 GO:0070696 GO:0071704 GO:0071944 GO:0080090 GO:0090056 GO:0090359 GO:0090693 GO:0099402 GO:0140096 GO:1901401 GO:1901404 GO:1901463 GO:1901564 GO:1902930 GO:1902931

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

U-box

Protein Analysis

1026

Amino Acids

114.0

Weight (kDa)

6.69

Isoelectric Point (pI)

45.9

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016312)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g22300 FvH4_4g22300
malus_domestica	MD13G1133000.v1.1
prunus_persica	Prupe.1G212500_v2.0.a1
pyrus_communis	pycom13g11540
rosa_chinensis	RchiOBHm_Chr4g0428541
rosa_laevigata	RLG00000007144
rosa_multiflora	Rmu_sc0002072.1_g000017
rosa_roxburghii	Rroxscaffold_5G00370240
rosa_rugosa	Rorug04G0226100
rosa_samantha	Rh4AG283600 Rh4BG289300 Rh4CG305200 Rh4DG286300
rosa_wichuraiana	Rw4G024620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	2854
AccBSI	CCGCTC	1 cut(s)	1041
AccI	GTMKAC	2 cut(s)	1900, 2540
AccII	CGCG	1 cut(s)	2717
AciI	CCGC	7 cut(s)	307, 313, 677, 1039, 2272, 2717, 2734
AclWI	GGATC	8 cut(s)	120, 469, 1224, 1775, 2893, 2923, 2974, 2987
AcoI	YGGCCR	3 cut(s)	3, 844, 2859
AcsI	RAATTY	5 cut(s)	470, 517, 550, 1001, 1165
AcuI	CTGAAG	6 cut(s)	489, 671, 1500, 1613, 1947, 2411
AcyI	GRCGYC	1 cut(s)	2591
AfaI	GTAC	6 cut(s)	51, 615, 721, 1743, 2286, 2456
AfiI	CCNNNNNNNGG	2 cut(s)	2707, 2854
AflII	CTTAAG	1 cut(s)	3011
AflIII	ACRYGT	2 cut(s)	839, 1773
AhlI	ACTAGT	1 cut(s)	2543
AjnI	CCWGG	6 cut(s)	149, 2166, 2480, 2700, 2847, 2931
AjuI	GAANNNNNNNTTGG	4 cut(s)	593, 625, 1931, 1963
Alw21I	GWGCWC	5 cut(s)	168, 595, 939, 1649, 1878
Alw26I	GTCTC	6 cut(s)	388, 981, 1193, 1526, 2425, 2560
AlwI	GGATC	8 cut(s)	120, 469, 1224, 1775, 2893, 2923, 2974, 2987
AlwNI	CAGNNNCTG	2 cut(s)	2756, 2771
Ama87I	CYCGRG	2 cut(s)	594, 1992
AoxI	GGCC	7 cut(s)	3, 84, 844, 1245, 1756, 2723, 2859
ApoI	RAATTY	5 cut(s)	470, 517, 550, 1001, 1165
AseI	ATTAAT	1 cut(s)	2184
Asp700I	GAANNNNTTC	2 cut(s)	605, 2615
AspLEI	GCGC	1 cut(s)	2400
AspS9I	GGNCC	2 cut(s)	1245, 2208
AsuC2I	CCSGG	1 cut(s)	3056
AsuHPI	GGTGA	6 cut(s)	1641, 2021, 2077, 2181, 2673, 2731
AsuII	TTCGAA	1 cut(s)	1252
AvaI	CYCGRG	2 cut(s)	594, 1992
AvaII	GGWCC	1 cut(s)	2208
AxyI	CCTNAGG	1 cut(s)	1527
BaeI	ACNNNNGTAYC	2 cut(s)	1841, 1874
BalI	TGGCCA	3 cut(s)	5, 846, 2861
BamHI	GGATCC	1 cut(s)	2979
BanII	GRGCYC	5 cut(s)	96, 168, 939, 1649, 1878
BarI	GAAGNNNNNNTAC	4 cut(s)	1256, 1288, 1704, 1736
BauI	CACGAG	1 cut(s)	95
BbsI	GAAGAC	2 cut(s)	657, 828
Bbv12I	GWGCWC	5 cut(s)	168, 595, 939, 1649, 1878
BceAI	ACGGC	1 cut(s)	569
BciT130I	CCWGG	6 cut(s)	151, 2168, 2482, 2702, 2849, 2933
BciVI	GTATCC	1 cut(s)	786
BclI	TGATCA	2 cut(s)	399, 2053
BcnI	CCSGG	1 cut(s)	3056
BcoDI	GTCTC	6 cut(s)	388, 981, 1193, 1526, 2425, 2560
BcuI	ACTAGT	1 cut(s)	2543
BfaI	CTAG	8 cut(s)	458, 537, 932, 1092, 1199, 2384, 2495, 2544
BfmI	CTRYAG	6 cut(s)	53, 715, 910, 1011, 1110, 1551
BfrI	CTTAAG	1 cut(s)	3011
BfuI	GTATCC	1 cut(s)	786
BlpI	GCTNAGC	1 cut(s)	2499
BmcAI	AGTACT	2 cut(s)	51, 2286
Bme1390I	CCNGG	7 cut(s)	151, 2168, 2482, 2702, 2849, 2933, 3056
Bme18I	GGWCC	1 cut(s)	2208
BmeT110I	CYCGRG	2 cut(s)	594, 1992
BmgT120I	GGNCC	2 cut(s)	1245, 2208
BmiI	GGNNCC	4 cut(s)	1246, 1831, 2124, 2981
BmrFI	CCNGG	7 cut(s)	151, 2168, 2482, 2702, 2849, 2933, 3056
BmrI	ACTGGG	3 cut(s)	224, 821, 2530
BmsI	GCATC	5 cut(s)	1285, 1557, 1623, 2008, 2642
BmuI	ACTGGG	3 cut(s)	224, 821, 2530
BpiI	GAAGAC	2 cut(s)	657, 828
BplI	GAGNNNNNCTC	3 cut(s)	740, 772, 3050
BpmI	CTGGAG	4 cut(s)	133, 161, 587, 2393
Bpu10I	CCTNAGC	2 cut(s)	419, 3035
Bpu1102I	GCTNAGC	1 cut(s)	2499
Bpu14I	TTCGAA	1 cut(s)	1252
BpuEI	CTTGAG	1 cut(s)	1559
BpuMI	CCSGG	1 cut(s)	3056
BsaBI	GATNNNNATC	3 cut(s)	2890, 2984, 3047
BsaHI	GRCGYC	1 cut(s)	2591
BsaI	GGTCTC	1 cut(s)	1526
BsaJI	CCNNGG	7 cut(s)	87, 1018, 2166, 2240, 2356, 2481, 2848
BsaXI	ACNNNNNCTCC	2 cut(s)	33, 63
Bsc4I	CCNNNNNNNGG	2 cut(s)	2707, 2854
Bse1I	ACTGG	8 cut(s)	219, 827, 1032, 1610, 1920, 1965, 2376, 2536
Bse21I	CCTNAGG	1 cut(s)	1527
Bse3DI	GCAATG	4 cut(s)	1299, 2085, 2514, 2792
Bse8I	GATNNNNATC	3 cut(s)	2890, 2984, 3047
BseBI	CCWGG	6 cut(s)	151, 2168, 2482, 2702, 2849, 2933
BseDI	CCNNGG	7 cut(s)	87, 1018, 2166, 2240, 2356, 2481, 2848
BseGI	GGATG	9 cut(s)	40, 361, 1300, 2023, 2175, 2251, 2326, 2352, 3051
BseJI	GATNNNNATC	3 cut(s)	2890, 2984, 3047
BseLI	CCNNNNNNNGG	2 cut(s)	2707, 2854
BseMI	GCAATG	4 cut(s)	1299, 2085, 2514, 2792
BseMII	CTCAG	6 cut(s)	153, 193, 410, 1541, 1797, 1961
BseNI	ACTGG	8 cut(s)	219, 827, 1032, 1610, 1920, 1965, 2376, 2536
BseRI	GAGGAG	3 cut(s)	167, 376, 2984
BseYI	CCCAGC	1 cut(s)	2117
BsgI	GTGCAG	1 cut(s)	222
Bsh1236I	CGCG	1 cut(s)	2717
BshFI	GGCC	7 cut(s)	5, 86, 846, 1247, 1758, 2725, 2861
BsiHKAI	GWGCWC	5 cut(s)	168, 595, 939, 1649, 1878
BsiHKCI	CYCGRG	2 cut(s)	594, 1992
BsiSI	CCGG	1 cut(s)	3056
BslI	CCNNNNNNNGG	2 cut(s)	2707, 2854
BsmAI	GTCTC	6 cut(s)	388, 981, 1193, 1526, 2425, 2560
BsmI	GAATGC	3 cut(s)	196, 607, 2383
BsnI	GGCC	7 cut(s)	5, 86, 846, 1247, 1758, 2725, 2861
Bso31I	GGTCTC	1 cut(s)	1526
BsoBI	CYCGRG	2 cut(s)	594, 1992
Bsp119I	TTCGAA	1 cut(s)	1252
Bsp1286I	GDGCHC	6 cut(s)	96, 168, 595, 939, 1649, 1878
Bsp1720I	GCTNAGC	1 cut(s)	2499
Bsp19I	CCATGG	1 cut(s)	1018
BspACI	CCGC	7 cut(s)	307, 313, 677, 1039, 2272, 2717, 2734
BspANI	GGCC	7 cut(s)	5, 86, 846, 1247, 1758, 2725, 2861
BspCNI	CTCAG	6 cut(s)	154, 192, 411, 1540, 1798, 1962
BspFNI	CGCG	1 cut(s)	2717
BspHI	TCATGA	1 cut(s)	2299
BspLI	GGNNCC	4 cut(s)	1246, 1831, 2124, 2981
BspMAI	CTGCAG	4 cut(s)	914, 1015, 1114, 1555
BspPI	GGATC	8 cut(s)	120, 469, 1224, 1775, 2893, 2923, 2974, 2987
BspT104I	TTCGAA	1 cut(s)	1252
BspTI	CTTAAG	1 cut(s)	3011
BspTNI	GGTCTC	1 cut(s)	1526
BsrBI	CCGCTC	1 cut(s)	1041
BsrDI	GCAATG	4 cut(s)	1299, 2085, 2514, 2792
BsrI	ACTGG	8 cut(s)	219, 827, 1032, 1610, 1920, 1965, 2376, 2536
BssECI	CCNNGG	7 cut(s)	87, 1018, 2166, 2240, 2356, 2481, 2848
BssNI	GRCGYC	1 cut(s)	2591
BssSI	CACGAG	1 cut(s)	95
BssT1I	CCWWGG	2 cut(s)	1018, 2356
Bst2BI	CACGAG	1 cut(s)	95
Bst2UI	CCWGG	6 cut(s)	151, 2168, 2482, 2702, 2849, 2933
Bst4CI	ACNGT	8 cut(s)	261, 382, 719, 1162, 1741, 1905, 2051, 2215
Bst6I	CTCTTC	4 cut(s)	1380, 1645, 2039, 2044
BstACI	GRCGYC	1 cut(s)	2591
BstAFI	CTTAAG	1 cut(s)	3011
BstAPI	GCANNNNNTGC	2 cut(s)	2771, 2831
BstBI	TTCGAA	1 cut(s)	1252
BstC8I	GCNNGC	9 cut(s)	274, 675, 1510, 1657, 1669, 1834, 1868, 2234, 2444
BstDEI	CTNAG	9 cut(s)	162, 179, 419, 1527, 1806, 1970, 2499, 2911, 3035
BstDSI	CCRYGG	2 cut(s)	87, 1018
BstENI	CCTNNNNNAGG	1 cut(s)	2705
BstF5I	GGATG	9 cut(s)	40, 361, 1300, 2023, 2175, 2251, 2326, 2352, 3051
BstFNI	CGCG	1 cut(s)	2717
BstHHI	GCGC	1 cut(s)	2400
BstMAI	GTCTC	6 cut(s)	388, 981, 1193, 1526, 2425, 2560
BstNI	CCWGG	6 cut(s)	151, 2168, 2482, 2702, 2849, 2933
BstNSI	RCATGY	6 cut(s)	486, 843, 1072, 1659, 1777, 2825
BstSCI	CCNGG	7 cut(s)	149, 2166, 2480, 2700, 2847, 2931, 3054
BstSFI	CTRYAG	6 cut(s)	53, 715, 910, 1011, 1110, 1551
BstUI	CGCG	1 cut(s)	2717
BstV2I	GAAGAC	2 cut(s)	657, 828
BstX2I	RGATCY	4 cut(s)	112, 2885, 2928, 2979
BstYI	RGATCY	4 cut(s)	112, 2885, 2928, 2979
Bsu36I	CCTNAGG	1 cut(s)	1527
BsuI	GTATCC	1 cut(s)	786
BsuRI	GGCC	7 cut(s)	5, 86, 846, 1247, 1758, 2725, 2861
BtgI	CCRYGG	2 cut(s)	87, 1018
BtsCI	GGATG	9 cut(s)	40, 361, 1300, 2023, 2175, 2251, 2326, 2352, 3051
BtsIMutI	CAGTG	9 cut(s)	212, 239, 1039, 1737, 1927, 1967, 2056, 2211, 2956
Cac8I	GCNNGC	9 cut(s)	274, 675, 1510, 1657, 1669, 1834, 1868, 2234, 2444
CaiI	CAGNNNCTG	2 cut(s)	2756, 2771
CciI	TCATGA	1 cut(s)	2299
CfoI	GCGC	1 cut(s)	2400
Cfr13I	GGNCC	2 cut(s)	1245, 2208
CseI	GACGC	2 cut(s)	2599, 2723
Csp6I	GTAC	6 cut(s)	50, 614, 720, 1742, 2285, 2455
CviQI	GTAC	6 cut(s)	50, 614, 720, 1742, 2285, 2455
DdeI	CTNAG	9 cut(s)	162, 179, 419, 1527, 1806, 1970, 2499, 2911, 3035
DraI	TTTAAA	1 cut(s)	999
EaeI	YGGCCR	3 cut(s)	3, 844, 2859
Eam1104I	CTCTTC	4 cut(s)	1380, 1645, 2039, 2044
EarI	CTCTTC	4 cut(s)	1380, 1645, 2039, 2044
EciI	GGCGGA	2 cut(s)	302, 692
Ecl136II	GAGCTC	4 cut(s)	166, 937, 1647, 1876
Eco130I	CCWWGG	2 cut(s)	1018, 2356
Eco24I	GRGCYC	5 cut(s)	96, 168, 939, 1649, 1878
Eco31I	GGTCTC	1 cut(s)	1526
Eco32I	GATATC	1 cut(s)	412
Eco47I	GGWCC	1 cut(s)	2208
Eco53kI	GAGCTC	4 cut(s)	166, 937, 1647, 1876
Eco57I	CTGAAG	6 cut(s)	489, 671, 1500, 1613, 1947, 2411
Eco81I	CCTNAGG	1 cut(s)	1527
Eco88I	CYCGRG	2 cut(s)	594, 1992
EcoICRI	GAGCTC	4 cut(s)	166, 937, 1647, 1876
EcoNI	CCTNNNNNAGG	1 cut(s)	2705
EcoRI	GAATTC	3 cut(s)	470, 517, 550
EcoRII	CCWGG	6 cut(s)	149, 2166, 2480, 2700, 2847, 2931
EcoRV	GATATC	1 cut(s)	412
EcoT14I	CCWWGG	2 cut(s)	1018, 2356
EcoT38I	GRGCYC	5 cut(s)	96, 168, 939, 1649, 1878
ErhI	CCWWGG	2 cut(s)	1018, 2356
FalI	AAGNNNNNCTT	4 cut(s)	119, 151, 2603, 2635
FbaI	TGATCA	2 cut(s)	399, 2053
FblI	GTMKAC	2 cut(s)	1900, 2540
FokI	GGATG	9 cut(s)	47, 368, 1307, 2030, 2162, 2258, 2333, 2339, 3038
FriOI	GRGCYC	5 cut(s)	96, 168, 939, 1649, 1878
FspBI	CTAG	8 cut(s)	458, 537, 932, 1092, 1199, 2384, 2495, 2544
GlaI	GCGC	1 cut(s)	2399
GsaI	CCCAGC	1 cut(s)	2121
GsuI	CTGGAG	4 cut(s)	133, 161, 587, 2393
HaeIII	GGCC	7 cut(s)	5, 86, 846, 1247, 1758, 2725, 2861
HapII	CCGG	1 cut(s)	3056
HgaI	GACGC	2 cut(s)	2599, 2723
HhaI	GCGC	1 cut(s)	2400
Hin1I	GRCGYC	1 cut(s)	2591
Hin6I	GCGC	1 cut(s)	2398
HinP1I	GCGC	1 cut(s)	2398
HindIII	AAGCTT	4 cut(s)	560, 1422, 2444, 2612
HinfI	GANTC	8 cut(s)	224, 494, 785, 979, 1023, 1810, 1942, 2753
HpaII	CCGG	1 cut(s)	3056
HphI	GGTGA	6 cut(s)	1641, 2021, 2077, 2181, 2673, 2731
Hpy166II	GTNNAC	9 cut(s)	228, 800, 1625, 1901, 2211, 2455, 2541, 2681, 2842
Hpy8I	GTNNAC	9 cut(s)	228, 800, 1625, 1901, 2211, 2455, 2541, 2681, 2842
Hpy99I	CGWCG	1 cut(s)	2593
HpyCH4III	ACNGT	8 cut(s)	261, 382, 719, 1162, 1741, 1905, 2051, 2215
HpyF3I	CTNAG	9 cut(s)	162, 179, 419, 1527, 1806, 1970, 2499, 2911, 3035
Hsp92I	GRCGYC	1 cut(s)	2591
HspAI	GCGC	1 cut(s)	2398
Ksp22I	TGATCA	2 cut(s)	399, 2053
LmnI	GCTCC	4 cut(s)	919, 1829, 1881, 3058
LweI	GCATC	5 cut(s)	1285, 1557, 1623, 2008, 2642
MaeI	CTAG	8 cut(s)	458, 537, 932, 1092, 1199, 2384, 2495, 2544
MaeIII	GTNAC	6 cut(s)	730, 835, 1034, 1413, 1915, 2744
MbiI	CCGCTC	1 cut(s)	1041
MfeI	CAATTG	4 cut(s)	498, 780, 2022, 2435
MflI	RGATCY	4 cut(s)	112, 2885, 2928, 2979
MhlI	GDGCHC	6 cut(s)	96, 168, 595, 939, 1649, 1878
MlsI	TGGCCA	3 cut(s)	5, 846, 2861
MluNI	TGGCCA	3 cut(s)	5, 846, 2861
MlyI	GAGTC	2 cut(s)	233, 1032
MmeI	TCCRAC	3 cut(s)	1293, 1519, 3013
Mox20I	TGGCCA	3 cut(s)	5, 846, 2861
MroXI	GAANNNNTTC	2 cut(s)	605, 2615
MscI	TGGCCA	3 cut(s)	5, 846, 2861
MslI	CAYNNNNRTG	3 cut(s)	793, 1654, 3003
Msp20I	TGGCCA	3 cut(s)	5, 846, 2861
MspA1I	CMGCKG	1 cut(s)	397
MspCI	CTTAAG	1 cut(s)	3011
MspI	CCGG	1 cut(s)	3056
MspR9I	CCNGG	7 cut(s)	151, 2168, 2482, 2702, 2849, 2933, 3056
MunI	CAATTG	4 cut(s)	498, 780, 2022, 2435
Mva1269I	GAATGC	3 cut(s)	196, 607, 2383
MvaI	CCWGG	6 cut(s)	151, 2168, 2482, 2702, 2849, 2933
MvnI	CGCG	1 cut(s)	2717
NciI	CCSGG	1 cut(s)	3056
NcoI	CCATGG	1 cut(s)	1018
NlaIV	GGNNCC	4 cut(s)	1246, 1831, 2124, 2981
NmuCI	GTSAC	2 cut(s)	1034, 1413
NspI	RCATGY	6 cut(s)	486, 843, 1072, 1659, 1777, 2825
NspV	TTCGAA	1 cut(s)	1252
PaeI	GCATGC	1 cut(s)	1659
PaeR7I	CTCGAG	2 cut(s)	594, 1992
PagI	TCATGA	1 cut(s)	2299
PciI	ACATGT	2 cut(s)	839, 1773
PctI	GAATGC	3 cut(s)	196, 607, 2383
PdmI	GAANNNNTTC	2 cut(s)	605, 2615
PfeI	GAWTC	6 cut(s)	494, 785, 979, 1810, 1942, 2753
PflMI	CCANNNNNTGG	1 cut(s)	2854
PfoI	TCCNGGA	2 cut(s)	149, 3054
PleI	GAGTC	2 cut(s)	232, 1031
PpsI	GAGTC	2 cut(s)	232, 1031
PscI	ACATGT	2 cut(s)	839, 1773
PshBI	ATTAAT	1 cut(s)	2184
Psp124BI	GAGCTC	4 cut(s)	168, 939, 1649, 1878
Psp6I	CCWGG	6 cut(s)	149, 2166, 2480, 2700, 2847, 2931
PspFI	CCCAGC	1 cut(s)	2117
PspGI	CCWGG	6 cut(s)	149, 2166, 2480, 2700, 2847, 2931
PspN4I	GGNNCC	4 cut(s)	1246, 1831, 2124, 2981
PspPI	GGNCC	2 cut(s)	1245, 2208
PspXI	VCTCGAGB	1 cut(s)	594
PstI	CTGCAG	4 cut(s)	914, 1015, 1114, 1555
PstNI	CAGNNNCTG	2 cut(s)	2756, 2771
PsuI	RGATCY	4 cut(s)	112, 2885, 2928, 2979
PvuII	CAGCTG	1 cut(s)	397
RsaI	GTAC	6 cut(s)	51, 615, 721, 1743, 2286, 2456
RsaNI	GTAC	6 cut(s)	50, 614, 720, 1742, 2285, 2455
RseI	CAYNNNNRTG	3 cut(s)	793, 1654, 3003
SacI	GAGCTC	4 cut(s)	168, 939, 1649, 1878
Sau96I	GGNCC	2 cut(s)	1245, 2208
ScaI	AGTACT	2 cut(s)	51, 2286
SchI	GAGTC	2 cut(s)	233, 1032
ScrFI	CCNGG	7 cut(s)	151, 2168, 2482, 2702, 2849, 2933, 3056
SduI	GDGCHC	6 cut(s)	96, 168, 595, 939, 1649, 1878
SfaNI	GCATC	5 cut(s)	1285, 1557, 1623, 2008, 2642
SfcI	CTRYAG	6 cut(s)	53, 715, 910, 1011, 1110, 1551
Sfr274I	CTCGAG	2 cut(s)	594, 1992
SfuI	TTCGAA	1 cut(s)	1252
SinI	GGWCC	1 cut(s)	2208
SlaI	CTCGAG	2 cut(s)	594, 1992
SmiMI	CAYNNNNRTG	3 cut(s)	793, 1654, 3003
SmlI	CTYRAG	4 cut(s)	594, 1574, 1992, 3011
SmoI	CTYRAG	4 cut(s)	594, 1574, 1992, 3011
SpeI	ACTAGT	1 cut(s)	2543
SphI	GCATGC	1 cut(s)	1659
SsiI	CCGC	7 cut(s)	307, 313, 677, 1039, 2272, 2717, 2734
SspMI	CTAG	8 cut(s)	458, 537, 932, 1092, 1199, 2384, 2495, 2544
SstI	GAGCTC	4 cut(s)	168, 939, 1649, 1878
StyD4I	CCNGG	7 cut(s)	149, 2166, 2480, 2700, 2847, 2931, 3054
StyI	CCWWGG	2 cut(s)	1018, 2356
TaaI	ACNGT	8 cut(s)	261, 382, 719, 1162, 1741, 1905, 2051, 2215
TaqI	TCGA	6 cut(s)	595, 1252, 1643, 1993, 2588, 2973
TatI	WGTACW	3 cut(s)	49, 719, 2284
TauI	GCSGC	1 cut(s)	309
TfiI	GAWTC	6 cut(s)	494, 785, 979, 1810, 1942, 2753
TscAI	CASTG	9 cut(s)	219, 246, 1039, 1744, 1927, 1974, 2056, 2218, 2956
TseFI	GTSAC	2 cut(s)	1034, 1413
Tsp45I	GTSAC	2 cut(s)	1034, 1413
TspGWI	ACGGA	1 cut(s)	506
TspRI	CASTG	9 cut(s)	219, 246, 1039, 1744, 1927, 1974, 2056, 2218, 2956
Van91I	CCANNNNNTGG	1 cut(s)	2854
Vha464I	CTTAAG	1 cut(s)	3011
VpaK11BI	GGWCC	1 cut(s)	2208
VspI	ATTAAT	1 cut(s)	2184
XagI	CCTNNNNNAGG	1 cut(s)	2705
XapI	RAATTY	5 cut(s)	470, 517, 550, 1001, 1165
XceI	RCATGY	6 cut(s)	486, 843, 1072, 1659, 1777, 2825
XcmI	CCANNNNNNNNNTGG	2 cut(s)	94, 1543
XhoI	CTCGAG	2 cut(s)	594, 1992
XmiI	GTMKAC	2 cut(s)	1900, 2540
XmnI	GAANNNNTTC	2 cut(s)	605, 2615
XspI	CTAG	8 cut(s)	458, 537, 932, 1092, 1199, 2384, 2495, 2544
ZrmI	AGTACT	2 cut(s)	51, 2286

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.