Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0016312

Physical Location & Seq

Genomic Coordinates Reverse (-)

50475899 .. 50480558

Gene Structure 4660 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G024620.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3090 bp

ATGTCTGGACTGGACTTCAGTATCGGAATGGAGGATGTTGGAATTGCAGTACTACAGGAATTATGGAATAAGGTGGCGTTTCAGACCATGGGGCTTGTTAGCGAAACAAGGGATATGTTGTTTGAAAAGGAAAGCTTTTTGGAGTTCTCCAGGAGCATTTCTGAGCTGAATATCCTCCTTAGCAGCTTAAATGCCCAGAAGGTTGAAAATGCACTTGGTTTAGAGTCCACGAGAGCTGCACTGACAACATTGAACCAGCAGTTGAAGAAAGCAAGCAAGATCATCAAGGATTACAAATGTGGAAGCCGCCTCCGTCTCTTGCTTAAATCTCACTCTATTCTCTTGCAGATGGAGGATGTAGCTAAAGATATTGCCAAGACTATCTCCACATTTCAGCTGGTCAATCTTGATACTGCACTGAACTTGAATACTATGACTAGCCAGATCATCAACAATCTGGGATCAATGGAATTCAGGTCAGCAGCTGCAACAGAAAAAATTGCTTCAGAGATGGAGACTTCAATATCAGAAAATACGAAGAATAGAGAGAATGCTAAAAAGCTCCTGGAGAAGATTGCTGAAGCTGTTGGTGCTAGGCCAAGTGCATCCTTGGTCCAAAATGAGTTGGCACTTCTGAAGCAAGAGAAAGAAGAGATGGAAGCTCAAAAAAAGCAGGCAGAGGCGCTTCAGCTATCTCAACTGATTGAGTTCCTTTACAGTACTGAAGTCGTTACAAGGCCAGTTGATGAGGAAACAGCTTCTACTCATCACCAACTATACCCGATTGGTTCATTCATATGCCAGTTGTGCAACAAGATGATGACAGACCCAGTAGCAATTCACTGTGGCCACAGCTTTGAGAGGGAGGCGATTCAGGAACACTTCAGACGAGGAGAGAGGAACTGCCCCACCTGCAGACAGGAGCTTTCATCTTTAGAGCTCACTCCTAACCTTTTGCTACGAAACTCTATAGAAGAATGGAATGAGAGAGACATGGACTTAAAATTCCAAGCTGCAGTGCCTAGAATCAAATCCAGTGAGCATTCACAACAGGACAAGGCCTTGGAGGATCTGCAACTTTTCCTGGAAAGGCCCAGATATGTGACCAAAGCTGCAGAGGAAGGGCTTGCGAAAAAGTTGGTGGAAATCTTAAAAGATGATAGGGTAAATGCTGGTGCAGCATTAAAATGCCTGTACTATCTAGCCAAATACAATGATGATCAGAAGGAAGCTATTGTCATAGCAGGAGGCATTCGCAGAATAGTGAAGCATATCTGTAAAGGTGGAAATGAATGCCATGCCCTCGGAGTCTTGATGAAGTTGTCTGAAAAGGAAAGCCTTGCAGAAAAGATGGGAAATACAAAAGATTGTATTCCCCTTGTGGTTTCTTTACTCCATATAAATAACCCAGATGTGTCTCAAAAAGCTAGTAAGGTGCTTCAGAACCTCTCCTCCAATGTCCATTTTGTTGTCAAGATGGCTGAAGCAGGACACTTCCAACCTTTTGTAGCTCGCTTCAACGATGCGGCTGGAGAAACCAGAACCCTGATGGCTGCAGCCTTGGTAAATATACAACTGAAGGAGAATAACATTGATGACTTAAGAGAACAGCAATTCATCCACAATCTAATTCAGATGCTCTCTTCCAGCTCCCCAGCATCCAAATCAGCTTGCCTAAAATGTATCAAGAAACTTGTAGCACACCACAAGATTGTAGAGCGCCTTCTGGAAGATGCTGTAACTATACCACATTTGCTTGGTCTCATCTCATTTAACAAGTTTGACCTGCACTTGAAACAAGGAGCTGCGGAGATTCTGGCTAAACTGATTGGAGATAGTGAGCAATTTGAGCAACCAAAATATCAGGGCTTGCAAGAACTGCAATCCAAGCACAATGTTAGTCTGCTGTTAGAGATTATGACCAGTGCCGAAGATCAAACCAAGATTCAGTTTTTACACCTACTGGTTGAGCTAAGCTCTAAATCAGAGATAGCCCGTGATTTAATACGAACAGACAACGATGCAATTGCTCAGCTCTTTTCATCCCTTTACAGCGACCATCCTGAGGTGAGAAGGTGGGTCATGAAACTTATATATTGCATATCTGAAGGTCATCCTGATGGAGTTCCACTACCAACGTCACCTGAAAAAGAAACAGCCATTACCAACCTAGCAACCATCCTCGTTAATTCACCAGATACCAAAGAAAGATCTGCTGCTGCAGGAATTATTAGCCAGCTTCCTCAGAATGATAGTAGCATTGATGAGATACTCCGAAAATCGGATGCGCTGAAAGCCATTTATGGGGTAATTTGCAGCATGGATGACGAAATTAGTGGGAACATAGGATCTTCCGACCAAGGCACATCTCTACTGGAGAATGCTCTAGCAGCCCTGTTACGCTTTACAGAACCCTCCAAACCCGATCTTCAGAGGCAGTTGGGAAAGCTTGAACTATACCCATCACTAGTTCGTGTACTGACACGGGGCAGCTCACTAGCTAAGCGGAGAACAGCAACTGCACTTGCCCAGCTATCCCTCTCCACCAGCCGGTCATTCTCTGATGAAACCATCATGGCACCACAAACCAAGACCTCCATGCCCTTCTTTCTCAAGCATGTCACCAGTATGTGGTGCTGCACAGCATCGTCACATAATTTCAGTTCATGTTCTGTTCACGGTGATGCTTGTTCACCAAGAGATACCTTCTGCCTGGTCAAGGCTGATGCGGTGAGGCCACTTGTGATGACTTTGAGTGACACAGAGTCGGGTGTGGCAGAGGCAGCTCTAATGGCACTTGAAACAATGCTTATCGATCACAGCACACGATCACAATCAACTGCTGTGATCGTGGACAGCCAGGGTGTGGCTGCCATTCTACACGTCTTGGACAAGGGATCTGTATCTGCCAAAACCAAAGCCTTAGACCTCTTTCAGAAGATCCTTTTACATACAAAGATCAGTGTACCGGTGATGCAGAGGTTTGAGAGGATCCTCATCCAATTTCTCAGCGATGATGACCTTAAGAAAAAAGCAGCTTTGGTACTTAGGCAGATGGGAATCATCCCAGAGCAGTCCTCATACTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006807 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009889 GO:0009890 GO:0009892 GO:0009894 GO:0009987 GO:0010115 GO:0010150 GO:0010271 GO:0010380 GO:0010565 GO:0016020 GO:0016567 GO:0016740 GO:0019216 GO:0019222 GO:0019538 GO:0019747 GO:0019787 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031329 GO:0032446 GO:0032501 GO:0032502 GO:0033612 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043455 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045827 GO:0045833 GO:0046890 GO:0048366 GO:0048367 GO:0048519 GO:0048523 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0051055 GO:0051171 GO:0051193 GO:0062012 GO:0062014 GO:0065007 GO:0070647 GO:0070696 GO:0071704 GO:0071944 GO:0080090 GO:0090056 GO:0090359 GO:0090693 GO:0099402 GO:0140096 GO:1901401 GO:1901404 GO:1901463 GO:1901564 GO:1902930 GO:1902931

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

U-box

Protein Analysis

1029

Amino Acids

114.21

Weight (kDa)

6.18

Isoelectric Point (pI)

46.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-Nse	PF11789	264 - 305	7e-06	Zinc-finger of the MIZ type in Nse subunit
U-box	PF04564	265 - 329	3.6e-13	U-box domain
ARM_ARMC5	PF24768	367 - 513	4.4e-06	ARMC5

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016312)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g22300 FvH4_4g22300
malus_domestica	MD13G1133000.v1.1
prunus_persica	Prupe.1G212500_v2.0.a1
pyrus_communis	pycom13g11540
rosa_chinensis	RchiOBHm_Chr4g0428541
rosa_laevigata	RLG00000007144
rosa_multiflora	Rmu_sc0002072.1_g000017
rosa_roxburghii	Rroxscaffold_5G00370240
rosa_rugosa	Rorug04G0226100
rosa_samantha	Rh4AG283600 Rh4BG289300 Rh4CG305200 Rh4DG286300
rosa_wichuraiana	Rw4G024620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	920
Acc36I	ACCTGC	2 cut(s)	920, 1794
AccB1I	GGYRCC	1 cut(s)	2578
AccB7I	CCANNNNNTGG	2 cut(s)	2631, 2866
AciI	CCGC	5 cut(s)	307, 1526, 1808, 2506, 2729
AclWI	GGATC	7 cut(s)	469, 1077, 2356, 2905, 2935, 2986, 2999
AcoI	YGGCCR	1 cut(s)	847
AcsI	RAATTY	2 cut(s)	470, 1004
AfaI	GTAC	6 cut(s)	51, 721, 1196, 2478, 2967, 3045
AfiI	CCNNNNNNNGG	7 cut(s)	2065, 2281, 2304, 2550, 2631, 2719, 2866
AflII	CTTAAG	2 cut(s)	1600, 3023
AflIII	ACRYGT	1 cut(s)	2881
AgeI	ACCGGT	1 cut(s)	2968
AhdI	GACNNNNNGTC	1 cut(s)	2764
AhlI	ACTAGT	1 cut(s)	2467
AjiI	CACGTC	1 cut(s)	2884
AjnI	CCWGG	5 cut(s)	149, 564, 1083, 2712, 2859
AjuI	GAANNNNNNNTTGG	2 cut(s)	198, 230
AloI	GAACNNNNNNTCC	2 cut(s)	1436, 1468
Alw21I	GWGCWC	1 cut(s)	942
Alw26I	GTCTC	5 cut(s)	320, 509, 984, 1422, 1766
AlwI	GGATC	7 cut(s)	469, 1077, 2356, 2905, 2935, 2986, 2999
AlwNI	CAGNNNCTG	2 cut(s)	485, 2519
AoxI	GGCC	6 cut(s)	596, 737, 847, 1059, 1091, 2735
ApoI	RAATTY	2 cut(s)	470, 1004
AsiGI	ACCGGT	1 cut(s)	2968
AspLEI	GCGC	3 cut(s)	685, 1722, 2290
AspS9I	GGNCC	2 cut(s)	613, 1092
AsuHPI	GGTGA	9 cut(s)	761, 2080, 2133, 2184, 2614, 2685, 2693, 2743, 2983
AvaII	GGWCC	1 cut(s)	613
AxyI	CCTNAGG	1 cut(s)	2064
BaeI	ACNNNNGTAYC	1 cut(s)	37
BalI	TGGCCA	1 cut(s)	849
BamHI	GGATCC	1 cut(s)	2991
BanI	GGYRCC	1 cut(s)	2578
BanII	GRGCYC	1 cut(s)	942
BarI	GAAGNNNNNNTAC	2 cut(s)	1707, 1739
BauI	CACGAG	1 cut(s)	229
Bbv12I	GWGCWC	1 cut(s)	942
BcgI	CGANNNNNNTGC	2 cut(s)	2009, 2043
BciT130I	CCWGG	5 cut(s)	151, 566, 1085, 2714, 2861
BclI	TGATCA	1 cut(s)	1219
BcoDI	GTCTC	5 cut(s)	320, 509, 984, 1422, 1766
BcuI	ACTAGT	1 cut(s)	2467
BfaI	CTAG	9 cut(s)	438, 594, 1023, 1202, 1428, 2171, 2387, 2468, 2498
BfmI	CTRYAG	7 cut(s)	53, 913, 969, 1014, 1113, 1554, 2220
BfoI	RGCGCY	2 cut(s)	686, 1723
BfrI	CTTAAG	2 cut(s)	1600, 3023
BfuAI	ACCTGC	2 cut(s)	920, 1794
BglII	AGATCT	1 cut(s)	2210
BlpI	GCTNAGC	3 cut(s)	1973, 2031, 2502
BmcAI	AGTACT	2 cut(s)	51, 721
Bme1390I	CCNGG	5 cut(s)	151, 566, 1085, 2714, 2861
Bme18I	GGWCC	1 cut(s)	613
BmeRI	GACNNNNNGTC	1 cut(s)	2764
BmgBI	CACGTC	1 cut(s)	2884
BmgT120I	GGNCC	2 cut(s)	613, 1092
BmiI	GGNNCC	2 cut(s)	2580, 2993
BmrFI	CCNGG	5 cut(s)	151, 566, 1085, 2714, 2861
BmrI	ACTGGG	1 cut(s)	824
BmuI	ACTGGG	1 cut(s)	824
BplI	GAGNNNNNCTC	3 cut(s)	1961, 1993, 3062
BpmI	CTGGAG	4 cut(s)	133, 587, 1551, 2396
Bpu10I	CCTNAGC	1 cut(s)	179
Bpu1102I	GCTNAGC	3 cut(s)	1973, 2031, 2502
BpuEI	CTTGAG	1 cut(s)	2597
Bsa29I	ATCGAT	1 cut(s)	2814
BsaBI	GATNNNNATC	4 cut(s)	2833, 2902, 2996, 3059
BsaI	GGTCTC	1 cut(s)	1766
BsaJI	CCNNGG	7 cut(s)	87, 609, 1062, 1303, 1560, 2359, 2860
BsaWI	WCCGGW	1 cut(s)	2968
BsaXI	ACNNNNNCTCC	2 cut(s)	1436, 1466
Bsc4I	CCNNNNNNNGG	7 cut(s)	2065, 2281, 2304, 2550, 2631, 2719, 2866
Bse118I	RCCGGY	2 cut(s)	2550, 2968
Bse1I	ACTGG	9 cut(s)	15, 740, 802, 830, 1035, 1923, 1968, 2379, 2625
Bse21I	CCTNAGG	1 cut(s)	2064
Bse8I	GATNNNNATC	4 cut(s)	2833, 2902, 2996, 3059
BseBI	CCWGG	5 cut(s)	151, 566, 1085, 2714, 2861
BseCI	ATCGAT	1 cut(s)	2814
BseDI	CCNNGG	7 cut(s)	87, 609, 1062, 1303, 1560, 2359, 2860
BseJI	GATNNNNATC	4 cut(s)	2833, 2902, 2996, 3059
BseLI	CCNNNNNNNGG	7 cut(s)	2065, 2281, 2304, 2550, 2631, 2719, 2866
BseMII	CTCAG	5 cut(s)	153, 2045, 2055, 2258, 3022
BseNI	ACTGG	9 cut(s)	15, 740, 802, 830, 1035, 1923, 1968, 2379, 2625
BseRI	GAGGAG	2 cut(s)	906, 1441
BseYI	CCCAGC	2 cut(s)	1654, 2529
BsgI	GTGCAG	6 cut(s)	222, 399, 1197, 1772, 2505, 2623
BshFI	GGCC	6 cut(s)	598, 739, 849, 1061, 1093, 2737
BshNI	GGYRCC	1 cut(s)	2578
BshTI	ACCGGT	1 cut(s)	2968
BshVI	ATCGAT	1 cut(s)	2814
BsiHKAI	GWGCWC	1 cut(s)	942
BsiSI	CCGG	2 cut(s)	2551, 2969
BslI	CCNNNNNNNGG	7 cut(s)	2065, 2281, 2304, 2550, 2631, 2719, 2866
BsmAI	GTCTC	5 cut(s)	320, 509, 984, 1422, 1766
BsmBI	CGTCTC	1 cut(s)	320
BsmI	GAATGC	5 cut(s)	556, 1042, 1251, 1298, 2386
BsnI	GGCC	6 cut(s)	598, 739, 849, 1061, 1093, 2737
Bso31I	GGTCTC	1 cut(s)	1766
Bsp1286I	GDGCHC	1 cut(s)	942
Bsp1720I	GCTNAGC	3 cut(s)	1973, 2031, 2502
Bsp19I	CCATGG	1 cut(s)	87
BspACI	CCGC	5 cut(s)	307, 1526, 1808, 2506, 2729
BspANI	GGCC	6 cut(s)	598, 739, 849, 1061, 1093, 2737
BspCNI	CTCAG	5 cut(s)	154, 2044, 2056, 2257, 3021
BspDI	ATCGAT	1 cut(s)	2814
BspHI	TCATGA	1 cut(s)	2082
BspLI	GGNNCC	2 cut(s)	2580, 2993
BspMAI	CTGCAG	5 cut(s)	917, 1018, 1117, 1558, 2224
BspMI	ACCTGC	2 cut(s)	920, 1794
BspPI	GGATC	7 cut(s)	469, 1077, 2356, 2905, 2935, 2986, 2999
BspT107I	GGYRCC	1 cut(s)	2578
BspTI	CTTAAG	2 cut(s)	1600, 3023
BspTNI	GGTCTC	1 cut(s)	1766
BsrFI	RCCGGY	2 cut(s)	2550, 2968
BsrI	ACTGG	9 cut(s)	15, 740, 802, 830, 1035, 1923, 1968, 2379, 2625
BssAI	RCCGGY	2 cut(s)	2550, 2968
BssECI	CCNNGG	7 cut(s)	87, 609, 1062, 1303, 1560, 2359, 2860
BssSI	CACGAG	1 cut(s)	229
BssT1I	CCWWGG	5 cut(s)	87, 609, 1062, 1560, 2359
Bst2BI	CACGAG	1 cut(s)	229
Bst2UI	CCWGG	5 cut(s)	151, 566, 1085, 2714, 2861
Bst4CI	ACNGT	3 cut(s)	719, 845, 2681
Bst6I	CTCTTC	2 cut(s)	645, 1648
BstAFI	CTTAAG	2 cut(s)	1600, 3023
BstAPI	GCANNNNNTGC	1 cut(s)	1879
BstC8I	GCNNGC	7 cut(s)	274, 675, 1128, 1513, 1672, 1871, 2237
BstDSI	CCRYGG	1 cut(s)	87
BstENI	CCTNNNNNAGG	1 cut(s)	2717
BstH2I	RGCGCY	2 cut(s)	686, 1723
BstHHI	GCGC	3 cut(s)	685, 1722, 2290
BstMAI	GTCTC	5 cut(s)	320, 509, 984, 1422, 1766
BstNI	CCWGG	5 cut(s)	151, 566, 1085, 2714, 2861
BstNSI	RCATGY	1 cut(s)	2621
BstSCI	CCNGG	5 cut(s)	149, 564, 1083, 2712, 2859
BstSFI	CTRYAG	7 cut(s)	53, 913, 969, 1014, 1113, 1554, 2220
BstX2I	RGATCY	6 cut(s)	1069, 2210, 2348, 2897, 2940, 2991
BstYI	RGATCY	6 cut(s)	1069, 2210, 2348, 2897, 2940, 2991
Bsu15I	ATCGAT	1 cut(s)	2814
Bsu36I	CCTNAGG	1 cut(s)	2064
BsuRI	GGCC	6 cut(s)	598, 739, 849, 1061, 1093, 2737
BsuTUI	ATCGAT	1 cut(s)	2814
BtgI	CCRYGG	1 cut(s)	87
BtgZI	GCGATG	1 cut(s)	3027
BtrI	CACGTC	1 cut(s)	2884
BtsI	GCAGTG	1 cut(s)	1023
BtsIMutI	CAGTG	7 cut(s)	239, 416, 841, 1023, 1042, 1930, 2968
BveI	ACCTGC	2 cut(s)	920, 1794
Cac8I	GCNNGC	7 cut(s)	274, 675, 1128, 1513, 1672, 1871, 2237
CaiI	CAGNNNCTG	2 cut(s)	485, 2519
CciI	TCATGA	1 cut(s)	2082
CfoI	GCGC	3 cut(s)	685, 1722, 2290
Cfr10I	RCCGGY	2 cut(s)	2550, 2968
Cfr13I	GGNCC	2 cut(s)	613, 1092
ClaI	ATCGAT	1 cut(s)	2814
Csp6I	GTAC	6 cut(s)	50, 720, 1195, 2477, 2966, 3044
CspAI	ACCGGT	1 cut(s)	2968
CviAII	CATG	9 cut(s)	88, 994, 1298, 2083, 2320, 2575, 2599, 2618, 2667
CviQI	GTAC	6 cut(s)	50, 720, 1195, 2477, 2966, 3044
DriI	GACNNNNNGTC	1 cut(s)	2764
EaeI	YGGCCR	1 cut(s)	847
Eam1104I	CTCTTC	2 cut(s)	645, 1648
Eam1105I	GACNNNNNGTC	1 cut(s)	2764
EarI	CTCTTC	2 cut(s)	645, 1648
Ecl136II	GAGCTC	1 cut(s)	940
Eco130I	CCWWGG	5 cut(s)	87, 609, 1062, 1560, 2359
Eco147I	AGGCCT	1 cut(s)	1061
Eco24I	GRGCYC	1 cut(s)	942
Eco31I	GGTCTC	1 cut(s)	1766
Eco47I	GGWCC	1 cut(s)	613
Eco53kI	GAGCTC	1 cut(s)	940
Eco81I	CCTNAGG	1 cut(s)	2064
EcoICRI	GAGCTC	1 cut(s)	940
EcoNI	CCTNNNNNAGG	1 cut(s)	2717
EcoRI	GAATTC	1 cut(s)	470
EcoRII	CCWGG	5 cut(s)	149, 564, 1083, 2712, 2859
EcoT14I	CCWWGG	5 cut(s)	87, 609, 1062, 1560, 2359
EcoT38I	GRGCYC	1 cut(s)	942
ErhI	CCWWGG	5 cut(s)	87, 609, 1062, 1560, 2359
Esp3I	CGTCTC	1 cut(s)	320
FaeI	CATG	9 cut(s)	91, 997, 1301, 2086, 2323, 2578, 2602, 2621, 2670
FalI	AAGNNNNNCTT	2 cut(s)	119, 151
FatI	CATG	9 cut(s)	87, 993, 1297, 2082, 2319, 2574, 2598, 2617, 2666
FauNDI	CATATG	1 cut(s)	797
FbaI	TGATCA	1 cut(s)	1219
FriOI	GRGCYC	1 cut(s)	942
FspBI	CTAG	9 cut(s)	438, 594, 1023, 1202, 1428, 2171, 2387, 2468, 2498
GlaI	GCGC	3 cut(s)	684, 1721, 2289
GsaI	CCCAGC	2 cut(s)	1658, 2533
GsuI	CTGGAG	4 cut(s)	133, 587, 1551, 2396
HaeII	RGCGCY	2 cut(s)	686, 1723
HaeIII	GGCC	6 cut(s)	598, 739, 849, 1061, 1093, 2737
HapII	CCGG	2 cut(s)	2551, 2969
HhaI	GCGC	3 cut(s)	685, 1722, 2290
Hin1II	CATG	9 cut(s)	91, 997, 1301, 2086, 2323, 2578, 2602, 2621, 2670
Hin6I	GCGC	3 cut(s)	683, 1720, 2288
HinP1I	GCGC	3 cut(s)	683, 1720, 2288
HindIII	AAGCTT	2 cut(s)	133, 2447
HinfI	GANTC	8 cut(s)	224, 871, 1026, 1308, 1813, 1945, 2765, 3060
HpaII	CCGG	2 cut(s)	2551, 2969
HphI	GGTGA	9 cut(s)	761, 2080, 2133, 2184, 2614, 2685, 2693, 2743, 2983
Hpy166II	GTNNAC	6 cut(s)	228, 2477, 2677, 2693, 2854, 2966
Hpy8I	GTNNAC	6 cut(s)	228, 2477, 2677, 2693, 2854, 2966
HpyAV	CCTTC	9 cut(s)	193, 1115, 1219, 1573, 1733, 2067, 2102, 2614, 2716
HpyCH4III	ACNGT	3 cut(s)	719, 845, 2681
HpyCH4IV	ACGT	2 cut(s)	2138, 2883
HpySE526I	ACGT	2 cut(s)	2138, 2883
Hsp92II	CATG	9 cut(s)	91, 997, 1301, 2086, 2323, 2578, 2602, 2621, 2670
HspAI	GCGC	3 cut(s)	683, 1720, 2288
Ksp22I	TGATCA	1 cut(s)	1219
LmnI	GCTCC	5 cut(s)	153, 567, 922, 1655, 1802
MaeI	CTAG	9 cut(s)	438, 594, 1023, 1202, 1428, 2171, 2387, 2468, 2498
MaeII	ACGT	2 cut(s)	2138, 2883
MaeIII	GTNAC	8 cut(s)	730, 1102, 1738, 2139, 2397, 2620, 2649, 2756
MfeI	CAATTG	1 cut(s)	2025
MflI	RGATCY	6 cut(s)	1069, 2210, 2348, 2897, 2940, 2991
MhlI	GDGCHC	1 cut(s)	942
MlsI	TGGCCA	1 cut(s)	849
MluNI	TGGCCA	1 cut(s)	849
MlyI	GAGTC	3 cut(s)	233, 1317, 2774
MmeI	TCCRAC	3 cut(s)	19, 1522, 2379
Mox20I	TGGCCA	1 cut(s)	849
MscI	TGGCCA	1 cut(s)	849
MslI	CAYNNNNRTG	5 cut(s)	796, 1186, 2118, 2627, 2682
Msp20I	TGGCCA	1 cut(s)	849
MspA1I	CMGCKG	2 cut(s)	397, 485
MspCI	CTTAAG	2 cut(s)	1600, 3023
MspI	CCGG	2 cut(s)	2551, 2969
MspR9I	CCNGG	5 cut(s)	151, 566, 1085, 2714, 2861
MunI	CAATTG	1 cut(s)	2025
Mva1269I	GAATGC	5 cut(s)	556, 1042, 1251, 1298, 2386
MvaI	CCWGG	5 cut(s)	151, 566, 1085, 2714, 2861
NcoI	CCATGG	1 cut(s)	87
NdeI	CATATG	1 cut(s)	797
NlaIII	CATG	9 cut(s)	91, 997, 1301, 2086, 2323, 2578, 2602, 2621, 2670
NlaIV	GGNNCC	2 cut(s)	2580, 2993
NmuCI	GTSAC	5 cut(s)	1102, 2139, 2620, 2649, 2756
NspI	RCATGY	1 cut(s)	2621
PagI	TCATGA	1 cut(s)	2082
PaqCI	CACCTGC	1 cut(s)	920
PceI	AGGCCT	1 cut(s)	1061
PctI	GAATGC	5 cut(s)	556, 1042, 1251, 1298, 2386
PfeI	GAWTC	5 cut(s)	871, 1026, 1813, 1945, 3060
PflMI	CCANNNNNTGG	2 cut(s)	2631, 2866
PfoI	TCCNGGA	3 cut(s)	149, 564, 1083
PinAI	ACCGGT	1 cut(s)	2968
PleI	GAGTC	3 cut(s)	232, 1316, 2773
PpsI	GAGTC	3 cut(s)	232, 1316, 2773
Psp124BI	GAGCTC	1 cut(s)	942
Psp6I	CCWGG	5 cut(s)	149, 564, 1083, 2712, 2859
PspFI	CCCAGC	2 cut(s)	1654, 2529
PspGI	CCWGG	5 cut(s)	149, 564, 1083, 2712, 2859
PspN4I	GGNNCC	2 cut(s)	2580, 2993
PspPI	GGNCC	2 cut(s)	613, 1092
PstI	CTGCAG	5 cut(s)	917, 1018, 1117, 1558, 2224
PstNI	CAGNNNCTG	2 cut(s)	485, 2519
PsuI	RGATCY	6 cut(s)	1069, 2210, 2348, 2897, 2940, 2991
PvuII	CAGCTG	2 cut(s)	397, 485
RsaI	GTAC	6 cut(s)	51, 721, 1196, 2478, 2967, 3045
RsaNI	GTAC	6 cut(s)	50, 720, 1195, 2477, 2966, 3044
RseI	CAYNNNNRTG	5 cut(s)	796, 1186, 2118, 2627, 2682
SacI	GAGCTC	1 cut(s)	942
Sau96I	GGNCC	2 cut(s)	613, 1092
ScaI	AGTACT	2 cut(s)	51, 721
SchI	GAGTC	3 cut(s)	233, 1317, 2774
ScrFI	CCNGG	5 cut(s)	151, 566, 1085, 2714, 2861
SduI	GDGCHC	1 cut(s)	942
SfcI	CTRYAG	7 cut(s)	53, 913, 969, 1014, 1113, 1554, 2220
SinI	GGWCC	1 cut(s)	613
SmiMI	CAYNNNNRTG	5 cut(s)	796, 1186, 2118, 2627, 2682
SmlI	CTYRAG	3 cut(s)	1600, 2612, 3023
SmoI	CTYRAG	3 cut(s)	1600, 2612, 3023
SpeI	ACTAGT	1 cut(s)	2467
SseBI	AGGCCT	1 cut(s)	1061
SsiI	CCGC	5 cut(s)	307, 1526, 1808, 2506, 2729
SspMI	CTAG	9 cut(s)	438, 594, 1023, 1202, 1428, 2171, 2387, 2468, 2498
SstI	GAGCTC	1 cut(s)	942
StuI	AGGCCT	1 cut(s)	1061
StyD4I	CCNGG	5 cut(s)	149, 564, 1083, 2712, 2859
StyI	CCWWGG	5 cut(s)	87, 609, 1062, 1560, 2359
TaaI	ACNGT	3 cut(s)	719, 845, 2681
TaiI	ACGT	2 cut(s)	2141, 2886
TaqI	TCGA	1 cut(s)	2814
TatI	WGTACW	4 cut(s)	49, 719, 1194, 2476
TauI	GCSGC	2 cut(s)	309, 1529
TfiI	GAWTC	5 cut(s)	871, 1026, 1813, 1945, 3060
TscAI	CASTG	7 cut(s)	246, 423, 848, 1023, 1042, 1930, 2968
TseFI	GTSAC	5 cut(s)	1102, 2139, 2620, 2649, 2756
Tsp45I	GTSAC	5 cut(s)	1102, 2139, 2620, 2649, 2756
TspGWI	ACGGA	1 cut(s)	302
TspRI	CASTG	7 cut(s)	246, 423, 848, 1023, 1042, 1930, 2968
Van91I	CCANNNNNTGG	2 cut(s)	2631, 2866
Vha464I	CTTAAG	2 cut(s)	1600, 3023
VpaK11BI	GGWCC	1 cut(s)	613
XagI	CCTNNNNNAGG	1 cut(s)	2717
XapI	RAATTY	2 cut(s)	470, 1004
XceI	RCATGY	1 cut(s)	2621
XcmI	CCANNNNNNNNNTGG	2 cut(s)	394, 1546
XspI	CTAG	9 cut(s)	438, 594, 1023, 1202, 1428, 2171, 2387, 2468, 2498
ZrmI	AGTACT	2 cut(s)	51, 721

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.