MD13G1188500.v1.1

membrane-associated kinase regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0016041
Physical Location & Seq
Reverse (-)
16051583 .. 16052785
1203 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1188500.v1.1.491

Sequence Viewer

Length: 1203 bp
ATGGAGTCGTCGAGGACGAGAAAAGATCCGACCAATGATATGACCAAACCCAATCCTCACCCTCACCGCCGTCACCTCCACCAAAAATCACAAACACTACCACCCTCACCCTCCCACTCCTTCCATTCCTCCTCCTCCTCCTCCGATTTCGAGTTCACCATCTCTCTCTCTCCTCGCAAATCCTCCAACGCCATCTGCCCCGCCGACGAGCTCTTCTACAAAGGCCAGATCCTCCCACTCCACCTCTCCCCTCGCCTCTCCATGGTCCGCAACCTCCGCCTCCCCTCCTCCGCATCCTACTCTACCTTCTCCGACACCACCACGTCGCGTGACTCCACCGGCAGCTCCTCCAACGAGTCACGTGACTCCACCTCCTCTTTTGCCAGCGACCTCGTACTCTTGGCCGACCAGTCATGCGACTCCTCCCGCCGCGACAGCTCTGTCGCCGAGGAAAACAACGAGGACTCGTTGTTCAAGCGCGCCTTCAACTACAACAACAACAAGTACGTGAGCGTCCACTCCAACGACAGCGTTTCGAAGAAGAAGTACTTTTCCTTGTCGATATTCTCCTCCGTGTTTAAGAAGGAGCCGAAAGTACAGAAGAACAACACGACAGATCCTCACAACAACGCTGCCAACCCCGCCACGGCGGGACTCGGGTCTAACAATGCAGTGAAACCTAAACGCATGAGCACTACTGCCAAGGAGGTTATTAGTAAGTACTTGAAGAAGGTGAAGCCCTTGTATGAAAAACTCTCCCAAAAACAACAACAACAACAACAAATGGGGGGAAGAGGAGGAGGATTGGGGATCATGACCACAACATCAATATCAACACCGAAAACATCCGACAAATGCACGGGGGTGGCGACGGCGAGGAACAATAATACAGATTGTGGTTCTAACAATCATGTATTAGTTTCTCATTCCTTCTCGGGTAACCTCAGGTACCCAAGAAGAAGAGAGCACATTTCTAGCTGCCCTTCTTCAATGAGGGGATCCCCGAGCCACTCTGGGGTTCTTTCCCGCACTTCGGGTCTAACGGGCTCGGGTGGTGGCGGCGCTAGTAGTAGCACGTCGTCGTACGGCGACAGATCGTCGATGGAGGAGCTTCAGAGTGCAATTCAAGGCGCAATTGCTCATTGCAAGAACTCCTTGGTTCATGGCAAGAGCACTATAACAATGGTCAGTCATGAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

401

Amino Acids

43.38

Weight (kDa)

9.74

Isoelectric Point (pI)

57.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016041)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G26230
fragaria_vesca FvH4_4g15080
malus_domestica MD13G1188500.v1.1 MD16G1188900.v1.1
prunus_persica Prupe.1G155600_v2.0.a1
pyrus_communis pycom13g16280
rosa_laevigata RLG00000007951
rosa_multiflora Rmu_co8515525.1_g000001
rosa_roxburghii Rroxscaffold_5G00360660
rosa_rugosa Rorug04G0144900
rosa_samantha Rh4AG207500 Rh4BG204600 Rh4CG218400 Rh4DG204500
rosa_wichuraiana Rw4G017540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 440
Acc65I GGTACC 1 cut(s) 948
AccB1I GGYRCC 1 cut(s) 948
AccII CGCG 3 cut(s) 328, 432, 480
AclWI GGATC 6 cut(s) 20, 223, 611, 818, 993, 1006
AcoI YGGCCR 1 cut(s) 402
AcuI CTGAAG 1 cut(s) 1097
AcvI CACGTG 1 cut(s) 362
AfaI GTAC 7 cut(s) 396, 506, 548, 597, 722, 950, 1085
AfiI CCNNNNNNNGG 4 cut(s) 262, 646, 1015, 1033
AgsI TTSAA 5 cut(s) 475, 487, 727, 990, 1127
AhdI GACNNNNNGTC 1 cut(s) 1096
AjiI CACGTC 2 cut(s) 324, 1077
AleI CACNNNNGTG 1 cut(s) 863
AloI GAACNNNNNNTCC 2 cut(s) 455, 487
AluBI AGCT 5 cut(s) 211, 345, 438, 978, 1111
AluI AGCT 5 cut(s) 211, 345, 438, 978, 1111
Alw21I GWGCWC 4 cut(s) 213, 695, 969, 1175
AlwI GGATC 6 cut(s) 20, 223, 611, 818, 993, 1006
Ama87I CYCGRG 4 cut(s) 656, 934, 1003, 1048
AoxI GGCC 2 cut(s) 223, 402
ApeKI GCWGC 3 cut(s) 342, 632, 978
Asp718I GGTACC 1 cut(s) 948
AspLEI GCGC 4 cut(s) 480, 482, 1064, 1133
AspS9I GGNCC 1 cut(s) 265
AsuHPI GGTGA 6 cut(s) 50, 56, 65, 99, 148, 745
AsuII TTCGAA 1 cut(s) 536
AvaI CYCGRG 4 cut(s) 656, 934, 1003, 1048
AvaII GGWCC 1 cut(s) 265
AxyI CCTNAGG 1 cut(s) 944
BamHI GGATCC 1 cut(s) 998
BanI GGYRCC 1 cut(s) 948
BanII GRGCYC 2 cut(s) 213, 1049
BarI GAAGNNNNNNTAC 2 cut(s) 530, 562
BbrPI CACGTG 1 cut(s) 362
Bbv12I GWGCWC 4 cut(s) 213, 695, 969, 1175
BbvI GCAGC 3 cut(s) 354, 619, 965
BccI CCATC 3 cut(s) 167, 200, 1096
BceAI ACGGC 4 cut(s) 54, 663, 888, 1102
BfaI CTAG 2 cut(s) 975, 1065
BfoI RGCGCY 1 cut(s) 1065
BisI GCNGC 5 cut(s) 343, 430, 633, 979, 1060
BlsI GCNGC 5 cut(s) 344, 431, 634, 980, 1061
BmcAI AGTACT 2 cut(s) 548, 722
Bme18I GGWCC 1 cut(s) 265
BmeRI GACNNNNNGTC 1 cut(s) 1096
BmeT110I CYCGRG 4 cut(s) 656, 934, 1003, 1048
BmgBI CACGTC 2 cut(s) 324, 1077
BmgT120I GGNCC 1 cut(s) 265
BmiI GGNNCC 3 cut(s) 588, 950, 1000
BmsI GCATC 1 cut(s) 302
BoxI GACNNNNGTC 1 cut(s) 658
Bpu14I TTCGAA 1 cut(s) 536
BsaAI YACGTR 2 cut(s) 362, 508
BsaJI CCNNGG 5 cut(s) 261, 447, 645, 702, 1155
BsaXI ACNNNNNCTCC 2 cut(s) 356, 386
Bsc4I CCNNNNNNNGG 4 cut(s) 262, 646, 1015, 1033
Bse118I RCCGGY 1 cut(s) 338
Bse1I ACTGG 1 cut(s) 409
Bse21I CCTNAGG 1 cut(s) 944
Bse3DI GCAATG 1 cut(s) 1141
BseDI CCNNGG 5 cut(s) 261, 447, 645, 702, 1155
BseGI GGATG 2 cut(s) 293, 845
BseLI CCNNNNNNNGG 4 cut(s) 262, 646, 1015, 1033
BseMI GCAATG 1 cut(s) 1141
BseMII CTCAG 1 cut(s) 958
BseNI ACTGG 1 cut(s) 409
BsePI GCGCGC 1 cut(s) 478
BseXI GCAGC 3 cut(s) 354, 619, 965
Bsh1236I CGCG 3 cut(s) 328, 432, 480
BshFI GGCC 2 cut(s) 225, 404
BshNI GGYRCC 1 cut(s) 948
BsiHKAI GWGCWC 4 cut(s) 213, 695, 969, 1175
BsiHKCI CYCGRG 4 cut(s) 656, 934, 1003, 1048
BsiSI CCGG 1 cut(s) 339
BsiWI CGTACG 1 cut(s) 1083
BslFI GGGAC 1 cut(s) 666
BslI CCNNNNNNNGG 4 cut(s) 262, 646, 1015, 1033
BsmFI GGGAC 1 cut(s) 666
BsnI GGCC 2 cut(s) 225, 404
BsoBI CYCGRG 4 cut(s) 656, 934, 1003, 1048
Bsp119I TTCGAA 1 cut(s) 536
Bsp1286I GDGCHC 5 cut(s) 213, 695, 969, 1049, 1175
Bsp143I GATC 6 cut(s) 25, 228, 616, 810, 998, 1094
Bsp19I CCATGG 1 cut(s) 261
BspANI GGCC 2 cut(s) 225, 404
BspCNI CTCAG 1 cut(s) 957
BspFNI CGCG 3 cut(s) 328, 432, 480
BspHI TCATGA 2 cut(s) 813, 1192
BspLI GGNNCC 3 cut(s) 588, 950, 1000
BspPI GGATC 6 cut(s) 20, 223, 611, 818, 993, 1006
BspQI GCTCTTC 1 cut(s) 218
BspT104I TTCGAA 1 cut(s) 536
BspT107I GGYRCC 1 cut(s) 948
BsrDI GCAATG 1 cut(s) 1141
BsrFI RCCGGY 1 cut(s) 338
BsrI ACTGG 1 cut(s) 409
BssAI RCCGGY 1 cut(s) 338
BssECI CCNNGG 5 cut(s) 261, 447, 645, 702, 1155
BssHII GCGCGC 1 cut(s) 478
BssMI GATC 6 cut(s) 25, 228, 616, 810, 998, 1094
BssT1I CCWWGG 3 cut(s) 261, 702, 1155
Bst6I CTCTTC 3 cut(s) 218, 787, 955
BstBAI YACGTR 2 cut(s) 362, 508
BstBI TTCGAA 1 cut(s) 536
BstC8I GCNNGC 2 cut(s) 385, 480
BstDEI CTNAG 1 cut(s) 944
BstDSI CCRYGG 2 cut(s) 261, 645
BstEII GGTNACC 1 cut(s) 938
BstF5I GGATG 2 cut(s) 293, 845
BstFNI CGCG 3 cut(s) 328, 432, 480
BstH2I RGCGCY 1 cut(s) 1065
BstHHI GCGC 4 cut(s) 480, 482, 1064, 1133
BstKTI GATC 6 cut(s) 28, 231, 619, 813, 1001, 1097
BstMBI GATC 6 cut(s) 25, 228, 616, 810, 998, 1094
BstMWI GCNNNNNNNGC 3 cut(s) 276, 435, 641
BstPAI GACNNNNGTC 1 cut(s) 658
BstPI GGTNACC 1 cut(s) 938
BstUI CGCG 3 cut(s) 328, 432, 480
BstV1I GCAGC 3 cut(s) 354, 619, 965
BstX2I RGATCY 4 cut(s) 25, 228, 616, 998
BstYI RGATCY 4 cut(s) 25, 228, 616, 998
Bsu36I CCTNAGG 1 cut(s) 944
BsuRI GGCC 2 cut(s) 225, 404
BtgI CCRYGG 2 cut(s) 261, 645
BtrI CACGTC 2 cut(s) 324, 1077
BtsCI GGATG 2 cut(s) 293, 845
BtsI GCAGTG 1 cut(s) 678
BtsIMutI CAGTG 1 cut(s) 678
Cac8I GCNNGC 2 cut(s) 385, 480
CciI TCATGA 2 cut(s) 813, 1192
CfoI GCGC 4 cut(s) 480, 482, 1064, 1133
Cfr10I RCCGGY 1 cut(s) 338
Cfr13I GGNCC 1 cut(s) 265
CseI GACGC 1 cut(s) 502
Csp6I GTAC 7 cut(s) 395, 505, 547, 596, 721, 949, 1084
CviAII CATG 7 cut(s) 262, 414, 688, 814, 911, 1163, 1193
CviQI GTAC 7 cut(s) 395, 505, 547, 596, 721, 949, 1084
DdeI CTNAG 1 cut(s) 944
DpnI GATC 6 cut(s) 27, 230, 618, 812, 1000, 1096
DpnII GATC 6 cut(s) 25, 228, 616, 810, 998, 1094
DrdI GACNNNNNNGTC 1 cut(s) 440
DriI GACNNNNNGTC 1 cut(s) 1096
DseDI GACNNNNNNGTC 1 cut(s) 440
EaeI YGGCCR 1 cut(s) 402
Eam1104I CTCTTC 3 cut(s) 218, 787, 955
Eam1105I GACNNNNNGTC 1 cut(s) 1096
EarI CTCTTC 3 cut(s) 218, 787, 955
EciI GGCGGA 1 cut(s) 266
Ecl136II GAGCTC 1 cut(s) 211
Eco130I CCWWGG 3 cut(s) 261, 702, 1155
Eco24I GRGCYC 2 cut(s) 213, 1049
Eco47I GGWCC 1 cut(s) 265
Eco53kI GAGCTC 1 cut(s) 211
Eco57I CTGAAG 1 cut(s) 1097
Eco72I CACGTG 1 cut(s) 362
Eco81I CCTNAGG 1 cut(s) 944
Eco88I CYCGRG 4 cut(s) 656, 934, 1003, 1048
Eco91I GGTNACC 1 cut(s) 938
EcoICRI GAGCTC 1 cut(s) 211
EcoO65I GGTNACC 1 cut(s) 938
EcoT14I CCWWGG 3 cut(s) 261, 702, 1155
EcoT38I GRGCYC 2 cut(s) 213, 1049
ErhI CCWWGG 3 cut(s) 261, 702, 1155
FaeI CATG 7 cut(s) 265, 417, 691, 817, 914, 1166, 1196
FalI AAGNNNNNCTT 6 cut(s) 467, 499, 533, 565, 1139, 1171
FaqI GGGAC 1 cut(s) 666
FatI CATG 7 cut(s) 261, 413, 687, 813, 910, 1162, 1192
FauI CCCGC 5 cut(s) 208, 434, 643, 649, 1034
Fnu4HI GCNGC 5 cut(s) 343, 430, 633, 979, 1060
FokI GGATG 2 cut(s) 280, 832
FriOI GRGCYC 2 cut(s) 213, 1049
Fsp4HI GCNGC 5 cut(s) 343, 430, 633, 979, 1060
FspBI CTAG 2 cut(s) 975, 1065
GlaI GCGC 4 cut(s) 479, 481, 1063, 1132
GluI GCNGC 5 cut(s) 343, 430, 633, 979, 1060
HaeII RGCGCY 1 cut(s) 1065
HaeIII GGCC 2 cut(s) 225, 404
HapII CCGG 1 cut(s) 339
HgaI GACGC 1 cut(s) 502
HhaI GCGC 4 cut(s) 480, 482, 1064, 1133
Hin1II CATG 7 cut(s) 265, 417, 691, 817, 914, 1166, 1196
Hin6I GCGC 4 cut(s) 478, 480, 1062, 1131
HinP1I GCGC 4 cut(s) 478, 480, 1062, 1131
HinfI GANTC 7 cut(s) 5, 332, 356, 365, 419, 464, 654
HpaII CCGG 1 cut(s) 339
HphI GGTGA 6 cut(s) 50, 56, 65, 99, 148, 745
Hpy166II GTNNAC 2 cut(s) 156, 517
Hpy188I TCNGA 5 cut(s) 30, 145, 313, 850, 1116
Hpy188III TCNNGA 2 cut(s) 814, 1193
Hpy8I GTNNAC 2 cut(s) 156, 517
Hpy99I CGWCG 7 cut(s) 13, 209, 328, 874, 1081, 1084, 1102
HpyAV CCTTC 7 cut(s) 130, 316, 493, 577, 724, 940, 993
HpyCH4IV ACGT 4 cut(s) 323, 361, 507, 1076
HpyCH4V TGCA 4 cut(s) 671, 858, 1121, 1146
HpyF10VI GCNNNNNNNGC 3 cut(s) 276, 435, 641
HpyF3I CTNAG 1 cut(s) 944
HpySE526I ACGT 4 cut(s) 323, 361, 507, 1076
Hsp92II CATG 7 cut(s) 265, 417, 691, 817, 914, 1166, 1196
HspAI GCGC 4 cut(s) 478, 480, 1062, 1131
KpnI GGTACC 1 cut(s) 952
Kzo9I GATC 6 cut(s) 25, 228, 616, 810, 998, 1094
LguI GCTCTTC 1 cut(s) 218
LmnI GCTCC 3 cut(s) 350, 586, 1108
LpnPI CCDG 6 cut(s) 239, 352, 397, 422, 931, 999
Lsp1109I GCAGC 3 cut(s) 354, 619, 965
LweI GCATC 1 cut(s) 302
MaeI CTAG 2 cut(s) 975, 1065
MaeII ACGT 4 cut(s) 323, 361, 507, 1076
MaeIII GTNAC 5 cut(s) 71, 329, 357, 362, 938
MalI GATC 6 cut(s) 27, 230, 618, 812, 1000, 1096
MboI GATC 6 cut(s) 25, 228, 616, 810, 998, 1094
MboII GAAGA 9 cut(s) 205, 550, 553, 613, 739, 804, 969, 972, 978
MfeI CAATTG 1 cut(s) 1134
MflI RGATCY 4 cut(s) 25, 228, 616, 998
MhlI GDGCHC 5 cut(s) 213, 695, 969, 1049, 1175
MluCI AATT 2 cut(s) 1122, 1134
MlyI GAGTC 7 cut(s) 14, 326, 359, 365, 413, 458, 648
MmeI TCCRAC 6 cut(s) 53, 210, 336, 375, 546, 873
MseI TTAA 1 cut(s) 579
MslI CAYNNNNRTG 1 cut(s) 863
MspI CCGG 1 cut(s) 339
MunI CAATTG 1 cut(s) 1134
MvnI CGCG 3 cut(s) 328, 432, 480
MwoI GCNNNNNNNGC 3 cut(s) 276, 435, 641
NcoI CCATGG 1 cut(s) 261
NdeII GATC 6 cut(s) 25, 228, 616, 810, 998, 1094
NlaIII CATG 7 cut(s) 265, 417, 691, 817, 914, 1166, 1196
NlaIV GGNNCC 3 cut(s) 588, 950, 1000
NmeAIII GCCGAG 1 cut(s) 472
NmuCI GTSAC 4 cut(s) 71, 329, 357, 362
NspV TTCGAA 1 cut(s) 536
OliI CACNNNNGTG 1 cut(s) 863
PagI TCATGA 2 cut(s) 813, 1192
PauI GCGCGC 1 cut(s) 478
PciSI GCTCTTC 1 cut(s) 218
PcsI WCGNNNNNNNCGW 2 cut(s) 14, 866
Pfl23II CGTACG 1 cut(s) 1083
PkrI GCNGC 5 cut(s) 344, 431, 634, 980, 1061
PleI GAGTC 7 cut(s) 13, 326, 359, 364, 413, 458, 648
PmaCI CACGTG 1 cut(s) 362
PmlI CACGTG 1 cut(s) 362
PpsI GAGTC 7 cut(s) 13, 326, 359, 364, 413, 458, 648
Ppu21I YACGTR 2 cut(s) 362, 508
PshAI GACNNNNGTC 1 cut(s) 658
Psp124BI GAGCTC 1 cut(s) 213
PspCI CACGTG 1 cut(s) 362
PspEI GGTNACC 1 cut(s) 938
PspLI CGTACG 1 cut(s) 1083
PspN4I GGNNCC 3 cut(s) 588, 950, 1000
PspPI GGNCC 1 cut(s) 265
PsuI RGATCY 4 cut(s) 25, 228, 616, 998
PteI GCGCGC 1 cut(s) 478
RsaI GTAC 7 cut(s) 396, 506, 548, 597, 722, 950, 1085
RsaNI GTAC 7 cut(s) 395, 505, 547, 596, 721, 949, 1084
RseI CAYNNNNRTG 1 cut(s) 863
SacI GAGCTC 1 cut(s) 213
SapI GCTCTTC 1 cut(s) 218
SaqAI TTAA 1 cut(s) 579
SatI GCNGC 5 cut(s) 343, 430, 633, 979, 1060
Sau3AI GATC 6 cut(s) 25, 228, 616, 810, 998, 1094
Sau96I GGNCC 1 cut(s) 265
ScaI AGTACT 2 cut(s) 548, 722
SchI GAGTC 7 cut(s) 14, 326, 359, 365, 413, 458, 648
SduI GDGCHC 5 cut(s) 213, 695, 969, 1049, 1175
SfaNI GCATC 1 cut(s) 302
SfuI TTCGAA 1 cut(s) 536
SinI GGWCC 1 cut(s) 265
SmiMI CAYNNNNRTG 1 cut(s) 863
Sse9I AATT 2 cut(s) 1122, 1134
SspMI CTAG 2 cut(s) 975, 1065
SstI GAGCTC 1 cut(s) 213
StyI CCWWGG 3 cut(s) 261, 702, 1155
TaiI ACGT 4 cut(s) 326, 364, 510, 1079
TaqI TCGA 5 cut(s) 11, 150, 536, 560, 1100
TasI AATT 2 cut(s) 1122, 1134
TatI WGTACW 3 cut(s) 546, 595, 720
TauI GCSGC 2 cut(s) 432, 1062
Tru1I TTAA 1 cut(s) 579
Tru9I TTAA 1 cut(s) 579
TscAI CASTG 1 cut(s) 678
TseFI GTSAC 4 cut(s) 71, 329, 357, 362
TseI GCWGC 3 cut(s) 342, 632, 978
Tsp45I GTSAC 4 cut(s) 71, 329, 357, 362
TspDTI ATGAA 2 cut(s) 762, 1151
TspGWI ACGGA 1 cut(s) 562
TspRI CASTG 1 cut(s) 678
VpaK11BI GGWCC 1 cut(s) 265
XspI CTAG 2 cut(s) 975, 1065
ZrmI AGTACT 2 cut(s) 548, 722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.