Prupe.1G155600_v2.0.a1

membrane-associated kinase regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0016041
Physical Location & Seq
Reverse (-)
12290310 .. 12292340
2031 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G155600.1

Sequence Viewer

Length: 1176 bp
ATGGAGATGAGGATGAAGAGAAAAGATGATGAGATGAACATGATCAAAGTGAAGAAGCATCATCATATTCATCATCCCCACCAAAAATCCCAAACCCTACCACCCTCCCCAACCCACTCTTTCTCTTCCTCCTCCTCCTCAGATTTCGAGTTCACAATCTCTCTCTCTCCTCGCAAATCCTCCAACACCCTCTGCCCCGCCGACGAGCTCTTCTACAAAGGCCAGCTCCTCCCCCTCCACCTCTCCCCCCGCCTCTCCATGGTCCGCACCCTCCACCTGCCCTCCTCCACGTCATCGTCTTACAACTGCTCCTCCTCCGAAACCACCACCTCACGTGACTCCACAGCCAGCAGCTCCAATGAGTCACGTGACTCCACCTCCTCCTTCGCCAGCGACCTCGGCCTCCTCGCCGACCAGTCCTGTGGCTCCTCTCGCCCCAGCTCCGTCGCCGAAGACACCAACGACGAGTCTTACAAGCGCCTCGGCAGCAGCAGCAAGTACGCGGTGCAGCACACCGCCAGCAACAACGATGCCTTTTCGAGGAAGAAGTACTTTTCCTTGTCGAGATTCTCGTCCGTGTTCAAGAAGGAGCCGAAAGTACAGAAAGACAACAATGTTCCAGATCACCAGCAGCAAAACAACAACAACATGGTGGGGCCCGGATCCAATAATGCATCATCAGCGAAGCCGAAACGTATGAGTTTGACTGCCAAGGAGGTTTTTAGCAAATACTTGAAGAAGGTGAAGCCCTTGTATGAAAAACTCTCCCAAAAACAGCAACAGAAAATGGGAGGAGGAGGCGGCGGCTTAAGCATCATCACGACCACAAAAACCTCAGCAGAAAAATGCGCCACGAGAAATAATAATAATAAAGATTGTGGTGGCAGCAGTCATGGTTTAGTTTCTCAGTCCTTCTCGGGTAACCTGAGGTACCCGAGAAGGAGGGGTAGCGTTTCGAGCTGCCCTTCTTCTATGAGGTGCTCCCCAAGCCACTCAGGCGTTCTCTCCCGTACTACCGGGTTAACGGGTTCGGGCTCAGGTTTGGGTACGAGTGGTGGCAACAGTTCAGCATCGTCCATGGAGGAGCTTCAGAGCGCAATTCAAGGCGCAATTGCTCATTGCAAGAACTCCTTGGTCGTTCAGAGCAAGAGCGCTGCTACGTGCCATGATAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

392

Amino Acids

42.17

Weight (kDa)

9.7

Isoelectric Point (pI)

58.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016041)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G26230
fragaria_vesca FvH4_4g15080
malus_domestica MD13G1188500.v1.1 MD16G1188900.v1.1
prunus_persica Prupe.1G155600_v2.0.a1
pyrus_communis pycom13g16280
rosa_laevigata RLG00000007951
rosa_multiflora Rmu_co8515525.1_g000001
rosa_roxburghii Rroxscaffold_5G00360660
rosa_rugosa Rorug04G0144900
rosa_samantha Rh4AG207500 Rh4BG204600 Rh4CG218400 Rh4DG204500
rosa_wichuraiana Rw4G017540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 285
Acc36I ACCTGC 1 cut(s) 285
Acc65I GGTACC 1 cut(s) 930
AccB1I GGYRCC 1 cut(s) 930
AccII CGCG 1 cut(s) 503
AciI CCGC 7 cut(s) 198, 250, 265, 503, 516, 801, 804
AclWI GGATC 2 cut(s) 657, 670
AcuI CTGAAG 1 cut(s) 1073
AcvI CACGTG 2 cut(s) 335, 368
AfaI GTAC 6 cut(s) 500, 551, 600, 932, 1012, 1048
AfeI AGCGCT 1 cut(s) 1153
AfiI CCNNNNNNNGG 2 cut(s) 259, 540
AflII CTTAAG 1 cut(s) 808
AgsI TTSAA 3 cut(s) 583, 736, 1103
AjiI CACGTC 1 cut(s) 291
AluBI AGCT 6 cut(s) 208, 226, 354, 441, 960, 1087
AluI AGCT 6 cut(s) 208, 226, 354, 441, 960, 1087
Alw21I GWGCWC 2 cut(s) 210, 983
AlwI GGATC 2 cut(s) 657, 670
Ama87I CYCGRG 2 cut(s) 916, 934
Aor51HI AGCGCT 1 cut(s) 1153
AoxI GGCC 3 cut(s) 220, 400, 656
ApaI GGGCCC 1 cut(s) 660
ApeKI GCWGC 9 cut(s) 351, 486, 489, 492, 508, 631, 885, 960, 1154
Asp718I GGTACC 1 cut(s) 930
AspLEI GCGC 5 cut(s) 480, 851, 1097, 1109, 1154
AspS9I GGNCC 3 cut(s) 262, 656, 657
AsuC2I CCSGG 2 cut(s) 660, 1018
AsuHPI GGTGA 2 cut(s) 617, 754
AvaI CYCGRG 2 cut(s) 916, 934
AvaII GGWCC 1 cut(s) 262
AxyI CCTNAGG 1 cut(s) 926
BaeGI GKGCMC 1 cut(s) 660
BamHI GGATCC 1 cut(s) 662
BanI GGYRCC 1 cut(s) 930
BanII GRGCYC 3 cut(s) 210, 660, 1037
BauI CACGAG 1 cut(s) 853
BbrPI CACGTG 2 cut(s) 335, 368
BbsI GAAGAC 1 cut(s) 459
Bbv12I GWGCWC 2 cut(s) 210, 983
BbvCI CCTCAGC 1 cut(s) 835
BbvI GCAGC 9 cut(s) 363, 498, 501, 504, 520, 643, 897, 947, 1141
BclI TGATCA 1 cut(s) 42
BcnI CCSGG 2 cut(s) 660, 1018
BfoI RGCGCY 2 cut(s) 481, 1155
BfrI CTTAAG 1 cut(s) 808
BfuAI ACCTGC 1 cut(s) 285
BglI GCCNNNNNGGC 1 cut(s) 996
BmcAI AGTACT 1 cut(s) 551
Bme1390I CCNGG 2 cut(s) 660, 1018
Bme18I GGWCC 1 cut(s) 262
BmeT110I CYCGRG 2 cut(s) 916, 934
BmgBI CACGTC 1 cut(s) 291
BmgT120I GGNCC 3 cut(s) 262, 656, 657
BmiI GGNNCC 6 cut(s) 427, 591, 657, 658, 664, 932
BmrFI CCNGG 2 cut(s) 660, 1018
BmsI GCATC 5 cut(s) 67, 520, 683, 822, 1079
BpiI GAAGAC 1 cut(s) 459
Bpu10I CCTNAGC 2 cut(s) 835, 1036
BpuMI CCSGG 2 cut(s) 660, 1018
BsaAI YACGTR 3 cut(s) 335, 368, 1161
BsaJI CCNNGG 6 cut(s) 258, 397, 481, 711, 1077, 1131
BsaXI ACNNNNNCTCC 8 cut(s) 266, 293, 296, 296, 323, 326, 362, 392
Bsc4I CCNNNNNNNGG 2 cut(s) 259, 540
Bse1I ACTGG 1 cut(s) 415
Bse21I CCTNAGG 1 cut(s) 926
Bse3DI GCAATG 1 cut(s) 1117
BseDI CCNNGG 6 cut(s) 258, 397, 481, 711, 1077, 1131
BseGI GGATG 2 cut(s) 18, 73
BseLI CCNNNNNNNGG 2 cut(s) 259, 540
BseMI GCAATG 1 cut(s) 1117
BseMII CTCAG 6 cut(s) 153, 849, 917, 920, 1008, 1050
BseNI ACTGG 1 cut(s) 415
BseSI GKGCMC 1 cut(s) 660
BseXI GCAGC 9 cut(s) 363, 498, 501, 504, 520, 643, 897, 947, 1141
BseYI CCCAGC 1 cut(s) 437
BsgI GTGCAG 1 cut(s) 527
Bsh1236I CGCG 1 cut(s) 503
BshFI GGCC 3 cut(s) 222, 402, 658
BshNI GGYRCC 1 cut(s) 930
BsiHKAI GWGCWC 2 cut(s) 210, 983
BsiHKCI CYCGRG 2 cut(s) 916, 934
BsiSI CCGG 2 cut(s) 660, 1017
BslI CCNNNNNNNGG 2 cut(s) 259, 540
BsnI GGCC 3 cut(s) 222, 402, 658
BsoBI CYCGRG 2 cut(s) 916, 934
Bsp120I GGGCCC 1 cut(s) 656
Bsp1286I GDGCHC 4 cut(s) 210, 660, 983, 1037
Bsp143I GATC 3 cut(s) 42, 622, 662
Bsp19I CCATGG 2 cut(s) 258, 1077
BspACI CCGC 7 cut(s) 198, 250, 265, 503, 516, 801, 804
BspANI GGCC 3 cut(s) 222, 402, 658
BspCNI CTCAG 6 cut(s) 152, 848, 918, 919, 1007, 1049
BspFNI CGCG 1 cut(s) 503
BspLI GGNNCC 6 cut(s) 427, 591, 657, 658, 664, 932
BspMI ACCTGC 1 cut(s) 285
BspPI GGATC 2 cut(s) 657, 670
BspQI GCTCTTC 1 cut(s) 215
BspT107I GGYRCC 1 cut(s) 930
BspTI CTTAAG 1 cut(s) 808
BsrDI GCAATG 1 cut(s) 1117
BsrI ACTGG 1 cut(s) 415
BssECI CCNNGG 6 cut(s) 258, 397, 481, 711, 1077, 1131
BssMI GATC 3 cut(s) 42, 622, 662
BssSI CACGAG 1 cut(s) 853
BssT1I CCWWGG 4 cut(s) 258, 711, 1077, 1131
Bst2BI CACGAG 1 cut(s) 853
Bst4CI ACNGT 1 cut(s) 1064
Bst6I CTCTTC 3 cut(s) 11, 130, 215
BstAFI CTTAAG 1 cut(s) 808
BstBAI YACGTR 3 cut(s) 335, 368, 1161
BstC8I GCNNGC 4 cut(s) 224, 349, 391, 520
BstDEI CTNAG 6 cut(s) 139, 835, 906, 926, 994, 1036
BstDSI CCRYGG 2 cut(s) 258, 1077
BstEII GGTNACC 1 cut(s) 920
BstENI CCTNNNNNAGG 1 cut(s) 538
BstF5I GGATG 2 cut(s) 18, 73
BstFNI CGCG 1 cut(s) 503
BstH2I RGCGCY 2 cut(s) 481, 1155
BstHHI GCGC 5 cut(s) 480, 851, 1097, 1109, 1154
BstKTI GATC 3 cut(s) 45, 625, 665
BstMBI GATC 3 cut(s) 42, 622, 662
BstMWI GCNNNNNNNGC 9 cut(s) 399, 432, 486, 492, 680, 810, 957, 987, 996
BstPI GGTNACC 1 cut(s) 920
BstSCI CCNGG 2 cut(s) 658, 1016
BstSLI GKGCMC 1 cut(s) 660
BstUI CGCG 1 cut(s) 503
BstV1I GCAGC 9 cut(s) 363, 498, 501, 504, 520, 643, 897, 947, 1141
BstV2I GAAGAC 1 cut(s) 459
BstX2I RGATCY 1 cut(s) 662
BstXI CCANNNNNNTGG 1 cut(s) 422
BstYI RGATCY 1 cut(s) 662
Bsu36I CCTNAGG 1 cut(s) 926
BsuRI GGCC 3 cut(s) 222, 402, 658
BtgI CCRYGG 2 cut(s) 258, 1077
BtrI CACGTC 1 cut(s) 291
BtsCI GGATG 2 cut(s) 18, 73
BveI ACCTGC 1 cut(s) 285
Cac8I GCNNGC 4 cut(s) 224, 349, 391, 520
CfoI GCGC 5 cut(s) 480, 851, 1097, 1109, 1154
Cfr13I GGNCC 3 cut(s) 262, 656, 657
Csp6I GTAC 6 cut(s) 499, 550, 599, 931, 1011, 1047
CviAII CATG 6 cut(s) 40, 259, 649, 893, 1078, 1166
CviQI GTAC 6 cut(s) 499, 550, 599, 931, 1011, 1047
DdeI CTNAG 6 cut(s) 139, 835, 906, 926, 994, 1036
DpnI GATC 3 cut(s) 44, 624, 664
DpnII GATC 3 cut(s) 42, 622, 662
Eam1104I CTCTTC 3 cut(s) 11, 130, 215
EarI CTCTTC 3 cut(s) 11, 130, 215
Ecl136II GAGCTC 1 cut(s) 208
Eco130I CCWWGG 4 cut(s) 258, 711, 1077, 1131
Eco24I GRGCYC 3 cut(s) 210, 660, 1037
Eco47I GGWCC 1 cut(s) 262
Eco47III AGCGCT 1 cut(s) 1153
Eco53kI GAGCTC 1 cut(s) 208
Eco57I CTGAAG 1 cut(s) 1073
Eco72I CACGTG 2 cut(s) 335, 368
Eco81I CCTNAGG 1 cut(s) 926
Eco88I CYCGRG 2 cut(s) 916, 934
Eco91I GGTNACC 1 cut(s) 920
EcoICRI GAGCTC 1 cut(s) 208
EcoNI CCTNNNNNAGG 1 cut(s) 538
EcoO109I RGGNCCY 1 cut(s) 656
EcoO65I GGTNACC 1 cut(s) 920
EcoT14I CCWWGG 4 cut(s) 258, 711, 1077, 1131
EcoT22I ATGCAT 1 cut(s) 676
EcoT38I GRGCYC 3 cut(s) 210, 660, 1037
ErhI CCWWGG 4 cut(s) 258, 711, 1077, 1131
FaeI CATG 6 cut(s) 43, 262, 652, 896, 1081, 1169
FalI AAGNNNNNCTT 4 cut(s) 536, 568, 1115, 1147
FatI CATG 6 cut(s) 39, 258, 648, 892, 1077, 1165
FauI CCCGC 2 cut(s) 205, 257
FbaI TGATCA 1 cut(s) 42
FokI GGATG 2 cut(s) 25, 60
FriOI GRGCYC 3 cut(s) 210, 660, 1037
GlaI GCGC 5 cut(s) 479, 850, 1096, 1108, 1153
GsaI CCCAGC 1 cut(s) 441
HaeII RGCGCY 2 cut(s) 481, 1155
HaeIII GGCC 3 cut(s) 222, 402, 658
HapII CCGG 2 cut(s) 660, 1017
HhaI GCGC 5 cut(s) 480, 851, 1097, 1109, 1154
Hin1II CATG 6 cut(s) 43, 262, 652, 896, 1081, 1169
Hin6I GCGC 5 cut(s) 478, 849, 1095, 1107, 1152
HinP1I GCGC 5 cut(s) 478, 849, 1095, 1107, 1152
HincII GTYRAC 1 cut(s) 1023
HindII GTYRAC 1 cut(s) 1023
HinfI GANTC 5 cut(s) 338, 362, 371, 467, 567
HpaI GTTAAC 1 cut(s) 1023
HpaII CCGG 2 cut(s) 660, 1017
HphI GGTGA 2 cut(s) 617, 754
Hpy166II GTNNAC 2 cut(s) 153, 1023
Hpy188I TCNGA 4 cut(s) 142, 319, 1092, 1143
Hpy188III TCNNGA 4 cut(s) 564, 583, 620, 820
Hpy8I GTNNAC 2 cut(s) 153, 1023
Hpy99I CGWCG 3 cut(s) 206, 449, 467
HpyAV CCTTC 6 cut(s) 394, 580, 733, 922, 933, 975
HpyCH4III ACNGT 1 cut(s) 1064
HpyCH4IV ACGT 5 cut(s) 290, 334, 367, 694, 1160
HpyCH4V TGCA 3 cut(s) 508, 674, 1122
HpyF10VI GCNNNNNNNGC 9 cut(s) 399, 432, 486, 492, 680, 810, 957, 987, 996
HpyF3I CTNAG 6 cut(s) 139, 835, 906, 926, 994, 1036
HpySE526I ACGT 5 cut(s) 290, 334, 367, 694, 1160
Hsp92II CATG 6 cut(s) 43, 262, 652, 896, 1081, 1169
HspAI GCGC 5 cut(s) 478, 849, 1095, 1107, 1152
KpnI GGTACC 1 cut(s) 934
Ksp22I TGATCA 1 cut(s) 42
KspAI GTTAAC 1 cut(s) 1023
Kzo9I GATC 3 cut(s) 42, 622, 662
LguI GCTCTTC 1 cut(s) 215
LmnI GCTCC 8 cut(s) 231, 314, 359, 431, 446, 589, 986, 1084
Lsp1109I GCAGC 9 cut(s) 363, 498, 501, 504, 520, 643, 897, 947, 1141
LweI GCATC 5 cut(s) 67, 520, 683, 822, 1079
MaeII ACGT 5 cut(s) 290, 334, 367, 694, 1160
MaeIII GTNAC 4 cut(s) 335, 363, 368, 920
MalI GATC 3 cut(s) 44, 624, 664
MboI GATC 3 cut(s) 42, 622, 662
MboII GAAGA 8 cut(s) 28, 64, 117, 202, 464, 556, 748, 960
MfeI CAATTG 1 cut(s) 1110
MflI RGATCY 1 cut(s) 662
MhlI GDGCHC 4 cut(s) 210, 660, 983, 1037
MluCI AATT 2 cut(s) 1098, 1110
MlyI GAGTC 4 cut(s) 332, 365, 371, 476
MmeI TCCRAC 1 cut(s) 207
Mph1103I ATGCAT 1 cut(s) 676
MseI TTAA 2 cut(s) 809, 1022
MspCI CTTAAG 1 cut(s) 808
MspI CCGG 2 cut(s) 660, 1017
MspR9I CCNGG 2 cut(s) 660, 1018
MunI CAATTG 1 cut(s) 1110
MvnI CGCG 1 cut(s) 503
MwoI GCNNNNNNNGC 9 cut(s) 399, 432, 486, 492, 680, 810, 957, 987, 996
NciI CCSGG 2 cut(s) 660, 1018
NcoI CCATGG 2 cut(s) 258, 1077
NdeII GATC 3 cut(s) 42, 622, 662
NlaIII CATG 6 cut(s) 43, 262, 652, 896, 1081, 1169
NlaIV GGNNCC 6 cut(s) 427, 591, 657, 658, 664, 932
NmeAIII GCCGAG 2 cut(s) 378, 462
NmuCI GTSAC 3 cut(s) 335, 363, 368
NsiI ATGCAT 1 cut(s) 676
PaqCI CACCTGC 1 cut(s) 285
PciSI GCTCTTC 1 cut(s) 215
PcsI WCGNNNNNNNCGW 1 cut(s) 569
PfeI GAWTC 1 cut(s) 567
PleI GAGTC 4 cut(s) 332, 365, 370, 475
PmaCI CACGTG 2 cut(s) 335, 368
PmlI CACGTG 2 cut(s) 335, 368
PpsI GAGTC 4 cut(s) 332, 365, 370, 475
Ppu21I YACGTR 3 cut(s) 335, 368, 1161
Psp124BI GAGCTC 1 cut(s) 210
PspCI CACGTG 2 cut(s) 335, 368
PspEI GGTNACC 1 cut(s) 920
PspFI CCCAGC 1 cut(s) 437
PspN4I GGNNCC 6 cut(s) 427, 591, 657, 658, 664, 932
PspOMI GGGCCC 1 cut(s) 656
PspPI GGNCC 3 cut(s) 262, 656, 657
PsuI RGATCY 1 cut(s) 662
RsaI GTAC 6 cut(s) 500, 551, 600, 932, 1012, 1048
RsaNI GTAC 6 cut(s) 499, 550, 599, 931, 1011, 1047
SacI GAGCTC 1 cut(s) 210
SapI GCTCTTC 1 cut(s) 215
SaqAI TTAA 2 cut(s) 809, 1022
Sau3AI GATC 3 cut(s) 42, 622, 662
Sau96I GGNCC 3 cut(s) 262, 656, 657
ScaI AGTACT 1 cut(s) 551
SchI GAGTC 4 cut(s) 332, 365, 371, 476
ScrFI CCNGG 2 cut(s) 660, 1018
SduI GDGCHC 4 cut(s) 210, 660, 983, 1037
SfaNI GCATC 5 cut(s) 67, 520, 683, 822, 1079
SinI GGWCC 1 cut(s) 262
SmlI CTYRAG 1 cut(s) 808
SmoI CTYRAG 1 cut(s) 808
Sse9I AATT 2 cut(s) 1098, 1110
SsiI CCGC 7 cut(s) 198, 250, 265, 503, 516, 801, 804
SstI GAGCTC 1 cut(s) 210
StyD4I CCNGG 2 cut(s) 658, 1016
StyI CCWWGG 4 cut(s) 258, 711, 1077, 1131
TaaI ACNGT 1 cut(s) 1064
TaiI ACGT 5 cut(s) 293, 337, 370, 697, 1163
TaqI TCGA 4 cut(s) 147, 539, 563, 956
TasI AATT 2 cut(s) 1098, 1110
TatI WGTACW 2 cut(s) 549, 598
TauI GCSGC 2 cut(s) 804, 807
TfiI GAWTC 1 cut(s) 567
Tru1I TTAA 2 cut(s) 809, 1022
Tru9I TTAA 2 cut(s) 809, 1022
TseFI GTSAC 3 cut(s) 335, 363, 368
TseI GCWGC 9 cut(s) 351, 486, 489, 492, 508, 631, 885, 960, 1154
Tsp45I GTSAC 3 cut(s) 335, 363, 368
TspDTI ATGAA 4 cut(s) 29, 50, 59, 771
TspGWI ACGGA 2 cut(s) 433, 565
Vha464I CTTAAG 1 cut(s) 808
VpaK11BI GGWCC 1 cut(s) 262
XagI CCTNNNNNAGG 1 cut(s) 538
ZrmI AGTACT 1 cut(s) 551
Zsp2I ATGCAT 1 cut(s) 676
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.