MD16G1188900.v1.1

membrane-associated kinase regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0016041
Physical Location & Seq
Reverse (-)
16432434 .. 16433615
1182 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1188900.v1.1.491

Sequence Viewer

Length: 1182 bp
ATGGAGACGTTGAGCACGGCAAAAGATCCGACCAATAATATGACCAAGCCCAATCCTCACCCTCACCGCCGTCACCTCCACCAAAAATCTCAAACCTTACCGCCCTCACCCACCCACTCCTTCCATTCCTCCTCCTCCTCTTCCTCCGACTTCGAGTTCACCATCTCTCTCTCCCCTCGCAAATCCTCCAACGCCGTCTGCCCCGCCGACGAGCTCTTCTACAAAGGACAGATCCTCCCTCTCCACCTCTCCCCTCGCCTCTCCATGGTCCGGACCCTCCTCCTCCCCTCCTCCGCGTCCTACTCTTCCTCCTCCGACACCACCACGTCGCGTGACTCCACCGGCAGCTCCTCCAACGAGTCACGAGATTCTACCTCCTCTTTTGCCAGCGACCTCGTGCTCTTGGCAGACCAATCATGCGACTCCTCCCGCCGCCCCAGCTCCGTCACGGAGGAAAACAACGAAGAGTCGTTGTTCAAGCGCGCCTTCAACTATAACAACAACAATAAGTACGTGAGCGTCCACTCTAACGACGGCGTTTCGAAGAAGAAGTACTTCTCGTTATCGAGATTCTCCTCCGTGTTCAAGAAGGAGCCGAAAGTGCAGAAAGACAACACTCTAGATCCTCACAACAACGCCGCCACGGCGGGGCTCGGATCTAACAATGCAGTGAAACCTAAACGCATGAGCACTACTGCCAAGGAGGTTATTAGTAAGTACTTGAAGAAGGTGAAGCCCTTGTATGAAAAATTCTCCCAAAAACAACAACAACAAAAAATGGGGGCAGGAGGAGGAGGACTGGGGGTCACGACCACAACAACGAAAACACCCGACAAATGCACGGCGACGGCGGCAAGGAATACTAATAAAGATTATGGCTCTAATAATCATGGATTATTTTCCCATTCCTTCTCCGGTAACCTGAGGTACCCTAGAAAGAGAGGGTGCGTTTCTAGCTGCCCTTCTTCAATGCGGGGATCTCCGAGCCACTCGGGGATTCTTTCCCGCACTGGGGGTCTAACGGGCTCGGGTGGTGGTGGCCCTAGTAGTGGCACTTCATCGTACGGGGACAGATCGTCGATGGAGGAGCTTCAGAGTGCAATTCAAGGCGCAATTGCTCATTGCAAGAACTCCTTGGTTCATGGCAAGAGCACTACAACCATGGTCAGTCATGAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

394

Amino Acids

42.32

Weight (kDa)

9.72

Isoelectric Point (pI)

55.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016041)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G26230
fragaria_vesca FvH4_4g15080
malus_domestica MD13G1188500.v1.1 MD16G1188900.v1.1
prunus_persica Prupe.1G155600_v2.0.a1
pyrus_communis pycom13g16280
rosa_laevigata RLG00000007951
rosa_multiflora Rmu_co8515525.1_g000001
rosa_roxburghii Rroxscaffold_5G00360660
rosa_rugosa Rorug04G0144900
rosa_samantha Rh4AG207500 Rh4BG204600 Rh4CG218400 Rh4DG204500
rosa_wichuraiana Rw4G017540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 927
AccB1I GGYRCC 1 cut(s) 927
AccII CGCG 3 cut(s) 296, 331, 483
AccIII TCCGGA 1 cut(s) 270
AclWI GGATC 5 cut(s) 20, 226, 617, 664, 985
AcsI RAATTY 1 cut(s) 749
AcuI CTGAAG 1 cut(s) 1076
AfaI GTAC 5 cut(s) 512, 554, 719, 929, 1064
AfiI CCNNNNNNNGG 6 cut(s) 265, 270, 648, 1011, 1012, 1049
AgsI TTSAA 6 cut(s) 478, 490, 586, 724, 969, 1106
AhdI GACNNNNNGTC 2 cut(s) 803, 1075
AjiI CACGTC 1 cut(s) 327
AluBI AGCT 5 cut(s) 214, 348, 441, 957, 1090
AluI AGCT 5 cut(s) 214, 348, 441, 957, 1090
Alw21I GWGCWC 5 cut(s) 17, 216, 402, 692, 1154
AlwI GGATC 5 cut(s) 20, 226, 617, 664, 985
Ama87I CYCGRG 2 cut(s) 991, 1027
Aor13HI TCCGGA 1 cut(s) 270
AoxI GGCC 1 cut(s) 1039
ApeKI GCWGC 2 cut(s) 345, 957
ApoI RAATTY 1 cut(s) 749
Asp700I GAANNNNTTC 1 cut(s) 554
Asp718I GGTACC 1 cut(s) 927
AspLEI GCGC 3 cut(s) 483, 485, 1112
AspS9I GGNCC 3 cut(s) 268, 273, 1040
AsuHPI GGTGA 6 cut(s) 50, 56, 65, 99, 151, 742
AsuII TTCGAA 1 cut(s) 542
AvaI CYCGRG 2 cut(s) 991, 1027
AvaII GGWCC 2 cut(s) 268, 273
AxyI CCTNAGG 1 cut(s) 923
BanI GGYRCC 1 cut(s) 927
BanII GRGCYC 3 cut(s) 216, 654, 1028
BarI GAAGNNNNNNTAC 4 cut(s) 536, 568, 1039, 1071
BauI CACGAG 2 cut(s) 363, 395
Bbv12I GWGCWC 5 cut(s) 17, 216, 402, 692, 1154
BbvI GCAGC 2 cut(s) 357, 944
BccI CCATC 2 cut(s) 170, 1075
BceAI ACGGC 7 cut(s) 33, 54, 179, 550, 660, 858, 864
BfaI CTAG 4 cut(s) 620, 933, 954, 1044
BglI GCCNNNNNGGC 1 cut(s) 644
BisI GCNGC 5 cut(s) 346, 433, 639, 852, 958
BlsI GCNGC 5 cut(s) 347, 434, 640, 853, 959
BmcAI AGTACT 2 cut(s) 554, 719
Bme18I GGWCC 2 cut(s) 268, 273
BmeRI GACNNNNNGTC 2 cut(s) 803, 1075
BmeT110I CYCGRG 2 cut(s) 991, 1027
BmgBI CACGTC 1 cut(s) 327
BmgT120I GGNCC 3 cut(s) 268, 273, 1040
BmiI GGNNCC 3 cut(s) 275, 594, 929
BmrI ACTGGG 2 cut(s) 809, 1020
BmuI ACTGGG 2 cut(s) 809, 1020
Bpu14I TTCGAA 1 cut(s) 542
BsaAI YACGTR 1 cut(s) 514
BsaJI CCNNGG 5 cut(s) 264, 642, 699, 1134, 1161
BsaWI WCCGGW 2 cut(s) 270, 914
BsaXI ACNNNNNCTCC 4 cut(s) 219, 249, 293, 323
Bsc4I CCNNNNNNNGG 6 cut(s) 265, 270, 648, 1011, 1012, 1049
Bse118I RCCGGY 1 cut(s) 341
Bse1I ACTGG 2 cut(s) 804, 1015
Bse21I CCTNAGG 1 cut(s) 923
Bse3DI GCAATG 1 cut(s) 1120
BseAI TCCGGA 1 cut(s) 270
BseDI CCNNGG 5 cut(s) 264, 642, 699, 1134, 1161
BseLI CCNNNNNNNGG 6 cut(s) 265, 270, 648, 1011, 1012, 1049
BseMI GCAATG 1 cut(s) 1120
BseMII CTCAG 1 cut(s) 914
BseNI ACTGG 2 cut(s) 804, 1015
BsePI GCGCGC 1 cut(s) 481
BseXI GCAGC 2 cut(s) 357, 944
BseYI CCCAGC 1 cut(s) 437
BsgI GTGCAG 1 cut(s) 623
Bsh1236I CGCG 3 cut(s) 296, 331, 483
BshFI GGCC 1 cut(s) 1041
BshNI GGYRCC 1 cut(s) 927
BsiHKAI GWGCWC 5 cut(s) 17, 216, 402, 692, 1154
BsiHKCI CYCGRG 2 cut(s) 991, 1027
BsiSI CCGG 3 cut(s) 271, 342, 915
BsiWI CGTACG 1 cut(s) 1062
BslFI GGGAC 1 cut(s) 1082
BslI CCNNNNNNNGG 6 cut(s) 265, 270, 648, 1011, 1012, 1049
BsmFI GGGAC 1 cut(s) 1082
BsnI GGCC 1 cut(s) 1041
BsoBI CYCGRG 2 cut(s) 991, 1027
Bsp119I TTCGAA 1 cut(s) 542
Bsp1286I GDGCHC 7 cut(s) 17, 216, 402, 654, 692, 1028, 1154
Bsp13I TCCGGA 1 cut(s) 270
Bsp143I GATC 6 cut(s) 25, 231, 622, 656, 977, 1073
Bsp19I CCATGG 2 cut(s) 264, 1161
BspANI GGCC 1 cut(s) 1041
BspCNI CTCAG 1 cut(s) 915
BspEI TCCGGA 1 cut(s) 270
BspFNI CGCG 3 cut(s) 296, 331, 483
BspHI TCATGA 1 cut(s) 1171
BspLI GGNNCC 3 cut(s) 275, 594, 929
BspPI GGATC 5 cut(s) 20, 226, 617, 664, 985
BspQI GCTCTTC 1 cut(s) 221
BspT104I TTCGAA 1 cut(s) 542
BspT107I GGYRCC 1 cut(s) 927
BsrDI GCAATG 1 cut(s) 1120
BsrFI RCCGGY 1 cut(s) 341
BsrI ACTGG 2 cut(s) 804, 1015
BssAI RCCGGY 1 cut(s) 341
BssECI CCNNGG 5 cut(s) 264, 642, 699, 1134, 1161
BssHII GCGCGC 1 cut(s) 481
BssMI GATC 6 cut(s) 25, 231, 622, 656, 977, 1073
BssSI CACGAG 2 cut(s) 363, 395
BssT1I CCWWGG 4 cut(s) 264, 699, 1134, 1161
Bst2BI CACGAG 2 cut(s) 363, 395
Bst6I CTCTTC 4 cut(s) 145, 221, 310, 459
BstBAI YACGTR 1 cut(s) 514
BstBI TTCGAA 1 cut(s) 542
BstC8I GCNNGC 2 cut(s) 388, 483
BstDEI CTNAG 1 cut(s) 923
BstDSI CCRYGG 3 cut(s) 264, 642, 1161
BstEII GGTNACC 1 cut(s) 917
BstFNI CGCG 3 cut(s) 296, 331, 483
BstHHI GCGC 3 cut(s) 483, 485, 1112
BstKTI GATC 6 cut(s) 28, 234, 625, 659, 980, 1076
BstMBI GATC 6 cut(s) 25, 231, 622, 656, 977, 1073
BstMWI GCNNNNNNNGC 5 cut(s) 438, 601, 644, 851, 954
BstPI GGTNACC 1 cut(s) 917
BstUI CGCG 3 cut(s) 296, 331, 483
BstV1I GCAGC 2 cut(s) 357, 944
BstX2I RGATCY 5 cut(s) 25, 231, 622, 656, 977
BstYI RGATCY 5 cut(s) 25, 231, 622, 656, 977
Bsu36I CCTNAGG 1 cut(s) 923
BsuRI GGCC 1 cut(s) 1041
BtgI CCRYGG 3 cut(s) 264, 642, 1161
BtrI CACGTC 1 cut(s) 327
BtsI GCAGTG 1 cut(s) 675
BtsIMutI CAGTG 2 cut(s) 675, 1008
Cac8I GCNNGC 2 cut(s) 388, 483
CciI TCATGA 1 cut(s) 1171
CfoI GCGC 3 cut(s) 483, 485, 1112
Cfr10I RCCGGY 1 cut(s) 341
Cfr13I GGNCC 3 cut(s) 268, 273, 1040
CseI GACGC 2 cut(s) 285, 508
Csp6I GTAC 5 cut(s) 511, 553, 718, 928, 1063
CviAII CATG 7 cut(s) 265, 417, 685, 890, 1142, 1162, 1172
CviQI GTAC 5 cut(s) 511, 553, 718, 928, 1063
DdeI CTNAG 1 cut(s) 923
DpnI GATC 6 cut(s) 27, 233, 624, 658, 979, 1075
DpnII GATC 6 cut(s) 25, 231, 622, 656, 977, 1073
DriI GACNNNNNGTC 2 cut(s) 803, 1075
Eam1104I CTCTTC 4 cut(s) 145, 221, 310, 459
Eam1105I GACNNNNNGTC 2 cut(s) 803, 1075
EarI CTCTTC 4 cut(s) 145, 221, 310, 459
Ecl136II GAGCTC 1 cut(s) 214
Eco130I CCWWGG 4 cut(s) 264, 699, 1134, 1161
Eco24I GRGCYC 3 cut(s) 216, 654, 1028
Eco47I GGWCC 2 cut(s) 268, 273
Eco53kI GAGCTC 1 cut(s) 214
Eco57I CTGAAG 1 cut(s) 1076
Eco81I CCTNAGG 1 cut(s) 923
Eco88I CYCGRG 2 cut(s) 991, 1027
Eco91I GGTNACC 1 cut(s) 917
EcoICRI GAGCTC 1 cut(s) 214
EcoO65I GGTNACC 1 cut(s) 917
EcoT14I CCWWGG 4 cut(s) 264, 699, 1134, 1161
EcoT38I GRGCYC 3 cut(s) 216, 654, 1028
ErhI CCWWGG 4 cut(s) 264, 699, 1134, 1161
FaeI CATG 7 cut(s) 268, 420, 688, 893, 1145, 1165, 1175
FalI AAGNNNNNCTT 6 cut(s) 470, 502, 539, 571, 1118, 1150
FaqI GGGAC 1 cut(s) 1082
FatI CATG 7 cut(s) 264, 416, 684, 889, 1141, 1161, 1171
FauI CCCGC 5 cut(s) 211, 437, 640, 966, 1013
Fnu4HI GCNGC 5 cut(s) 346, 433, 639, 852, 958
FriOI GRGCYC 3 cut(s) 216, 654, 1028
Fsp4HI GCNGC 5 cut(s) 346, 433, 639, 852, 958
FspBI CTAG 4 cut(s) 620, 933, 954, 1044
GlaI GCGC 3 cut(s) 482, 484, 1111
GluI GCNGC 5 cut(s) 346, 433, 639, 852, 958
GsaI CCCAGC 1 cut(s) 441
HaeIII GGCC 1 cut(s) 1041
HapII CCGG 3 cut(s) 271, 342, 915
HgaI GACGC 2 cut(s) 285, 508
HhaI GCGC 3 cut(s) 483, 485, 1112
Hin1II CATG 7 cut(s) 268, 420, 688, 893, 1145, 1165, 1175
Hin6I GCGC 3 cut(s) 481, 483, 1110
HinP1I GCGC 3 cut(s) 481, 483, 1110
HinfI GANTC 7 cut(s) 335, 359, 368, 422, 467, 570, 997
HpaII CCGG 3 cut(s) 271, 342, 915
HphI GGTGA 6 cut(s) 50, 56, 65, 99, 151, 742
Hpy166II GTNNAC 2 cut(s) 159, 523
Hpy188I TCNGA 6 cut(s) 30, 148, 316, 656, 984, 1095
Hpy188III TCNNGA 7 cut(s) 271, 363, 567, 586, 620, 808, 1172
Hpy8I GTNNAC 2 cut(s) 159, 523
Hpy99I CGWCG 5 cut(s) 212, 331, 536, 850, 1081
HpyAV CCTTC 6 cut(s) 130, 496, 583, 721, 919, 972
HpyCH4IV ACGT 3 cut(s) 8, 326, 513
HpyCH4V TGCA 5 cut(s) 604, 668, 840, 1100, 1125
HpyF10VI GCNNNNNNNGC 5 cut(s) 438, 601, 644, 851, 954
HpyF3I CTNAG 1 cut(s) 923
HpySE526I ACGT 3 cut(s) 8, 326, 513
Hsp92II CATG 7 cut(s) 268, 420, 688, 893, 1145, 1165, 1175
HspAI GCGC 3 cut(s) 481, 483, 1110
Kpn2I TCCGGA 1 cut(s) 270
KpnI GGTACC 1 cut(s) 931
Kzo9I GATC 6 cut(s) 25, 231, 622, 656, 977, 1073
LguI GCTCTTC 1 cut(s) 221
LmnI GCTCC 4 cut(s) 353, 446, 592, 1087
LpnPI CCDG 9 cut(s) 284, 355, 400, 451, 771, 785, 928, 935, 996
Lsp1109I GCAGC 2 cut(s) 357, 944
MaeI CTAG 4 cut(s) 620, 933, 954, 1044
MaeII ACGT 3 cut(s) 8, 326, 513
MaeIII GTNAC 6 cut(s) 71, 332, 360, 445, 805, 917
MalI GATC 6 cut(s) 27, 233, 624, 658, 979, 1075
MboI GATC 6 cut(s) 25, 231, 622, 656, 977, 1073
MboII GAAGA 8 cut(s) 132, 208, 297, 476, 556, 559, 736, 957
MfeI CAATTG 1 cut(s) 1113
MflI RGATCY 5 cut(s) 25, 231, 622, 656, 977
MhlI GDGCHC 7 cut(s) 17, 216, 402, 654, 692, 1028, 1154
MluCI AATT 3 cut(s) 749, 1101, 1113
MlyI GAGTC 4 cut(s) 329, 368, 416, 476
MmeI TCCRAC 5 cut(s) 53, 171, 213, 339, 378
MroI TCCGGA 1 cut(s) 270
MroXI GAANNNNTTC 1 cut(s) 554
MspI CCGG 3 cut(s) 271, 342, 915
MunI CAATTG 1 cut(s) 1113
MvnI CGCG 3 cut(s) 296, 331, 483
MwoI GCNNNNNNNGC 5 cut(s) 438, 601, 644, 851, 954
NcoI CCATGG 2 cut(s) 264, 1161
NdeII GATC 6 cut(s) 25, 231, 622, 656, 977, 1073
NlaIII CATG 7 cut(s) 268, 420, 688, 893, 1145, 1165, 1175
NlaIV GGNNCC 3 cut(s) 275, 594, 929
NmuCI GTSAC 5 cut(s) 71, 332, 360, 445, 805
NspV TTCGAA 1 cut(s) 542
PagI TCATGA 1 cut(s) 1171
PauI GCGCGC 1 cut(s) 481
PciSI GCTCTTC 1 cut(s) 221
PdmI GAANNNNTTC 1 cut(s) 554
PfeI GAWTC 3 cut(s) 368, 570, 997
Pfl23II CGTACG 1 cut(s) 1062
PkrI GCNGC 5 cut(s) 347, 434, 640, 853, 959
PleI GAGTC 4 cut(s) 329, 367, 416, 475
PpsI GAGTC 4 cut(s) 329, 367, 416, 475
Ppu21I YACGTR 1 cut(s) 514
Psp124BI GAGCTC 1 cut(s) 216
PspEI GGTNACC 1 cut(s) 917
PspFI CCCAGC 1 cut(s) 437
PspLI CGTACG 1 cut(s) 1062
PspN4I GGNNCC 3 cut(s) 275, 594, 929
PspPI GGNCC 3 cut(s) 268, 273, 1040
PsuI RGATCY 5 cut(s) 25, 231, 622, 656, 977
PteI GCGCGC 1 cut(s) 481
RsaI GTAC 5 cut(s) 512, 554, 719, 929, 1064
RsaNI GTAC 5 cut(s) 511, 553, 718, 928, 1063
SacI GAGCTC 1 cut(s) 216
SapI GCTCTTC 1 cut(s) 221
SatI GCNGC 5 cut(s) 346, 433, 639, 852, 958
Sau3AI GATC 6 cut(s) 25, 231, 622, 656, 977, 1073
Sau96I GGNCC 3 cut(s) 268, 273, 1040
ScaI AGTACT 2 cut(s) 554, 719
SchI GAGTC 4 cut(s) 329, 368, 416, 476
SduI GDGCHC 7 cut(s) 17, 216, 402, 654, 692, 1028, 1154
SfuI TTCGAA 1 cut(s) 542
SinI GGWCC 2 cut(s) 268, 273
Sse9I AATT 3 cut(s) 749, 1101, 1113
SspMI CTAG 4 cut(s) 620, 933, 954, 1044
SstI GAGCTC 1 cut(s) 216
StyI CCWWGG 4 cut(s) 264, 699, 1134, 1161
TaiI ACGT 3 cut(s) 11, 329, 516
TaqI TCGA 4 cut(s) 153, 542, 566, 1079
TasI AATT 3 cut(s) 749, 1101, 1113
TatI WGTACW 2 cut(s) 552, 717
TauI GCSGC 3 cut(s) 435, 641, 854
TfiI GAWTC 3 cut(s) 368, 570, 997
TscAI CASTG 2 cut(s) 675, 1015
TseFI GTSAC 5 cut(s) 71, 332, 360, 445, 805
TseI GCWGC 2 cut(s) 345, 957
Tsp45I GTSAC 5 cut(s) 71, 332, 360, 445, 805
TspDTI ATGAA 3 cut(s) 759, 1047, 1130
TspGWI ACGGA 3 cut(s) 433, 464, 568
TspRI CASTG 2 cut(s) 675, 1015
VpaK11BI GGWCC 2 cut(s) 268, 273
XapI RAATTY 1 cut(s) 749
XbaI TCTAGA 1 cut(s) 619
XmnI GAANNNNTTC 1 cut(s) 554
XspI CTAG 4 cut(s) 620, 933, 954, 1044
ZrmI AGTACT 2 cut(s) 554, 719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.