Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0012732

Physical Location & Seq

Genomic Coordinates Forward (+)

16382002 .. 16386368

Gene Structure 4367 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1191200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2640 bp

ATGGAAGCATATTGTTTGAAGGACAAGGAGAAGGACAATGACGACGAGAAGCCAGATTACATGGTGAGTGGGAGCAACAGAATCAGAGGAAAAGTTGTGTTGATGAAGAAGAATGTGTTGGACTTCAACGACCTCAAAGCTTCGTTCGTTGATAGAATCCATGAGCTCTTGGGCAAAGGTGTTTCCATGCAGCTCATTAGTGCCACTCATCCTGAAGCAGCAGCAAATGGATTGAAACCGAGAGGGAAGCTTGGAAAGGTTGCTTACCTGGAGAAATGGGCTACAACCATTACATCGTTGTCGGCCGGTGAAACTAGTTTTACCATTTCCTTGGATTGGGATGAGTCCCATGGAGCTCCCGGCGCTTTGCTGATCAGAAACCATCACCATAGCCAATTCTACCTCAAGACAATAACATTAGAAGATGTTCCCGAGCACGGTCAACTCCATTTTGTCTGCAATTCCTGGGTATACCCAGCACATCGTTACAAGTACAATCGGATATTTTTCCCCAACAAGGCCTACCTGCCATCTCAAACACCTGAGCTACTACACCCTTACAGGGAAGAAGAACTCGCCAATCTCCGAGGGAGTGGATCAGGCGAGCTAAAGGAGTGGGACAGGGTCTATGACTATGCTTACTACAATGATTTAGGGAGTCCTGACAAGGGTCCAAAATATGCCCGCCCTATACTTGGTGGGTCGTACGAGTATCCATATCCAAGAAGAGGAAGAACTGGTCGAAAAACAACTAAAACCGACCCCAATTCCGAGAGCAGACTGCCGCTTCTAAGTCTAGACATTTATGTTCCAAGAGATGAGCGGTTTGGCCACGTGAAGTTTTCCGATTTTCTTGCTTATGGCCTGAAATCCCTAGTTCAAATATTGCTTCCAGAACTCAAGTCTTTGTGTGATAAAACTATCAATGAGTTTGACACCTTTGACGACGTAGTTAATCTCTACGAGGGAGGTATCAAGTTACCAAATGGGCCTACACTGAAGAAAATAAGGGAGCGCATCCCTTGGGAGCTGCTTAAGGAACTGATTCGCTCAGATGGCGAGCGATTTCTCAAGTTTCCAATGCCTGATGTGATTAAAAAAGATAGGTCTGCATGGAGGACAGATAAAGAATTTGGACGAGAAATGCTAGCAGGAGTGAACCCTGTTAACATCACCCGCCTCAAAGAGTTTCCCCCAGTCAGTAAGCTAGACCCTAAAGTATATGGGAATCAGAACAGTTCGATACGAGAAGAGTACATAAAAGGCAACATGAATGGCCTCACTGTTGAAGAGGCAACTGAAAAGAACAGGCTATTCATATTAGATCATCATGATGCATTAATGTTATACCTGAGGCAAATAAACTCAACGGACACAAAGACGTATGCCACAAGAACTCTCCTCTTGCTGCAAGAAGACGGGACGTTGAAGCCTTTGGCAATTGAGCTAAGCTTACCACATCCACAAGGGGACCGTCATGGGGCTGTCAGCAAAGTTTTCACTCCACCAGGACAAGGCAATGAACGCATCGAGGAGTCGGTGTGGCAGCTTGCCAAAGCTTATGCTGCTGTAAACGATTCTGGCTATCATCAGCTTATCAGCCACTGGTTAAACACACATGCTGTGATAGAGCCATTTGTGATCACAACAAATAGACAGTTGAGTGTACTTCACCCGATACATAAGCTTTTGCATCCGCACTTCCGGGACACAATGAACATAAATGCCCTGGCCAGGCAAATCCTCATCAATGCCGGTGGGGTGCTTGAGAAAACAGTCTTCCCAGGTCGATTCTCCATGGAAATGTCGGCTGTTATTTATAAGAGCTGGGTATTTACCGAGCAGGCATTACCTGCTGACTTGCTCAAGAGAGGAATGGCAGTTCCAGATACGACTTGTCCTCATGGCCTGAGACTTCTGATTGAAGACTACCCGTTTGCTGTTGATGGGCTAGAAATTTGGTCGGCGATTGAAACTTGGGTTAATGAATACTGCTCTTTGTACTACCAATCGGACGATGTGGTCCAAGGTGATTCTGAACTCCAGAACTGGTGGACAGAGCTCCGCAATATAGGCCACGGTGACAAGAAGAATGAACCATGGTGGCCTAAGATGCAAACAAGAGCTGAACTTATCGAATCATGTACCATAATCATATGGGTGGCTTCTGCCCTCCATGCAGCAGTCAATTTCGGGCAATACTCTTACGCTGGCTACCTCCCTAACCGCCCAACTGTAAGCCGACGTTTCATGCCTGAGCCAGGCACTGCTGAATATTATGAGCTTGTGTCCTCCCCTGACACAGCTTTCCTTAAAACAATTACAGCTCAGTTCCAAACTCTCCTTGGTGTATCTCTGATAGAGATTTTATCACGACATTCGACTGATGAGGTTTATCTAGGGCAAAACGATACTCCTGAGTGGACTTCAGATGCTGAAGCACTAGCAGCATTTGCAAGATTCGGAGAGAAGCTAATGGACATCGAAAAGAGAATCGATGCAAGGAACAAGGATAATAGATTGAAGAACAGAGTTGGGCCTGTAGACGTGCCTTATACCTTGCTTCACCCTAACACATCTGACAAGAGTAGAGAGGGTGGACTGACTGGAAAAGGAATTCCCAACAGTGTCTCAATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006629 GO:0007275 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009653 GO:0009791 GO:0009987 GO:0010015 GO:0010101 GO:0010102 GO:0010311 GO:0016491 GO:0016701 GO:0016702 GO:0022622 GO:0030258 GO:0031347 GO:0031348 GO:0032501 GO:0032502 GO:0034440 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044255 GO:0044424 GO:0044444 GO:0044464 GO:0048364 GO:0048519 GO:0048527 GO:0048528 GO:0048583 GO:0048585 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0051213 GO:0055114 GO:0065007 GO:0071704 GO:0080134 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:1900366 GO:1905392 GO:1905393 GO:1990136 GO:2000068

KEGG Pathways

Metabolic & Signaling

ko00591 ko00592 ko01100 ko01110 map00591 map00592 map01100 map01110

Pfam Domains

Protein Families

Lipoxygenase PLAT

Protein Analysis

880

Amino Acids

99.94

Weight (kDa)

6.41

Isoelectric Point (pI)

33.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PLAT	PF01477	79 - 170	1e-19	PLAT/LH2 domain
Lipoxygenase	PF00305	184 - 857	0.0	Lipoxygenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012732)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G22400
fragaria_vesca	FvH4_4g15250 FvH4_4g15250 FvH4_4g15250
malus_domestica	MD13G1191200.v1.1 MD16G1191300.v1.1
prunus_persica	Prupe.1G011400_v2.0.a1 Prupe.1G011400_v2.0.a1 Prupe.1G011400_v2.0.a1
pyrus_communis	pycom13g16410 pycom16g16100
rosa_chinensis	RchiOBHm_Chr4g0416591
rosa_laevigata	RLG00000007969
rosa_roxburghii	Rroxscaffold_5G00360470
rosa_rugosa	Rorug04G0143200
rosa_samantha	Rh4BG203100 Rh4CG216500
rosa_wichuraiana	Rw4G017400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1821
AasI	GACNNNNNNGTC	1 cut(s)	2021
Acc36I	ACCTGC	2 cut(s)	534, 1861
AccBSI	CCGCTC	1 cut(s)	823
AccI	GTMKAC	2 cut(s)	471, 2544
AciI	CCGC	7 cut(s)	685, 785, 823, 1177, 1697, 2065, 2227
AclWI	GGATC	1 cut(s)	604
AcoI	YGGCCR	3 cut(s)	303, 829, 1731
AcsI	RAATTY	3 cut(s)	1130, 1956, 2616
AcuI	CTGAAG	4 cut(s)	234, 1019, 2412, 2457
AcvI	CACGTG	1 cut(s)	835
AfaI	GTAC	6 cut(s)	494, 707, 1256, 1668, 2003, 2146
AflII	CTTAAG	1 cut(s)	1034
AgsI	TTSAA	9 cut(s)	19, 127, 235, 881, 1289, 1429, 1925, 1973, 2524
AhlI	ACTAGT	1 cut(s)	314
AjiI	CACGTC	1 cut(s)	2548
AjnI	CCWGG	7 cut(s)	267, 464, 1507, 1728, 1733, 1783, 2260
AjuI	GAANNNNNNNTTGG	2 cut(s)	101, 133
AloI	GAACNNNNNNTCC	2 cut(s)	1866, 1898
Alw21I	GWGCWC	4 cut(s)	168, 358, 438, 2064
Alw26I	GTCTC	2 cut(s)	1906, 2635
AlwI	GGATC	1 cut(s)	604
AlwNI	CAGNNNCTG	3 cut(s)	1605, 2267, 2435
Ama87I	CYCGRG	1 cut(s)	431
ApeKI	GCWGC	9 cut(s)	190, 218, 221, 1030, 1408, 1546, 1565, 2180, 2447
ApoI	RAATTY	3 cut(s)	1130, 1956, 2616
ArsI	GACNNNNNNTTYG	2 cut(s)	1919, 1951
AseI	ATTAAT	1 cut(s)	1340
Asp700I	GAANNNNTTC	2 cut(s)	426, 1044
AspLEI	GCGC	2 cut(s)	365, 1017
AspS9I	GGNCC	5 cut(s)	671, 989, 1471, 2023, 2537
AsuC2I	CCSGG	2 cut(s)	360, 1706
AsuHPI	GGTGA	8 cut(s)	76, 320, 377, 1165, 1664, 2042, 2093, 2558
AsuNHI	GCTAGC	1 cut(s)	1147
AvaI	CYCGRG	1 cut(s)	431
AvaII	GGWCC	3 cut(s)	671, 1471, 2023
AxyI	CCTNAGG	1 cut(s)	1352
BalI	TGGCCA	2 cut(s)	831, 1733
BanII	GRGCYC	3 cut(s)	168, 358, 2064
BarI	GAAGNNNNNNTAC	2 cut(s)	41, 73
BbrPI	CACGTG	1 cut(s)	835
BbsI	GAAGAC	3 cut(s)	1422, 1771, 1932
Bbv12I	GWGCWC	4 cut(s)	168, 358, 438, 2064
BbvI	GCAGC	9 cut(s)	202, 230, 233, 1017, 1395, 1552, 1558, 2192, 2459
BccI	CCATC	4 cut(s)	390, 538, 1049, 1940
BcgI	CGANNNNNNTGC	2 cut(s)	836, 870
BciT130I	CCWGG	7 cut(s)	269, 466, 1509, 1730, 1735, 1785, 2262
BciVI	GTATCC	1 cut(s)	723
BclI	TGATCA	2 cut(s)	372, 1641
BcnI	CCSGG	2 cut(s)	360, 1706
BcoDI	GTCTC	2 cut(s)	1906, 2635
BcuI	ACTAGT	1 cut(s)	314
BfaI	CTAG	8 cut(s)	315, 797, 875, 1148, 1208, 1952, 2399, 2444
BfmI	CTRYAG	1 cut(s)	2541
BfoI	RGCGCY	1 cut(s)	366
BfrI	CTTAAG	1 cut(s)	1034
BfuAI	ACCTGC	2 cut(s)	534, 1861
BfuI	GTATCC	1 cut(s)	723
BlpI	GCTNAGC	1 cut(s)	1448
Bme1390I	CCNGG	9 cut(s)	269, 360, 466, 1509, 1706, 1730, 1735, 1785, 2262
Bme18I	GGWCC	3 cut(s)	671, 1471, 2023
BmeT110I	CYCGRG	1 cut(s)	431
BmgBI	CACGTC	1 cut(s)	2548
BmgT120I	GGNCC	5 cut(s)	671, 989, 1471, 2023, 2537
BmiI	GGNNCC	2 cut(s)	672, 1472
BmrFI	CCNGG	9 cut(s)	269, 360, 466, 1509, 1706, 1730, 1735, 1785, 2262
BmrI	ACTGGG	1 cut(s)	1190
BmsI	GCATC	7 cut(s)	1026, 1324, 1536, 1702, 2103, 2422, 2488
BmtI	GCTAGC	1 cut(s)	1151
BmuI	ACTGGG	1 cut(s)	1190
BoxI	GACNNNNGTC	1 cut(s)	669
BpiI	GAAGAC	3 cut(s)	1422, 1771, 1932
BpmI	CTGGAG	2 cut(s)	290, 2027
Bpu10I	CCTNAGC	2 cut(s)	543, 2256
Bpu1102I	GCTNAGC	1 cut(s)	1448
BpuEI	CTTGAG	5 cut(s)	389, 884, 1055, 1787, 1850
BpuMI	CCSGG	2 cut(s)	360, 1706
Bsa29I	ATCGAT	1 cut(s)	2496
BsaAI	YACGTR	1 cut(s)	835
BsaXI	ACNNNNNCTCC	2 cut(s)	1526, 1556
Bse118I	RCCGGY	2 cut(s)	305, 1754
Bse1I	ACTGG	5 cut(s)	742, 1196, 1610, 2054, 2611
Bse21I	CCTNAGG	1 cut(s)	1352
Bse3DI	GCAATG	1 cut(s)	1525
BseBI	CCWGG	7 cut(s)	269, 466, 1509, 1730, 1735, 1785, 2262
BseCI	ATCGAT	1 cut(s)	2496
BseGI	GGATG	5 cut(s)	208, 346, 1017, 1459, 1693
BseMI	GCAATG	1 cut(s)	1525
BseMII	CTCAG	7 cut(s)	534, 1065, 1343, 1901, 2247, 2342, 2409
BseNI	ACTGG	5 cut(s)	742, 1196, 1610, 2054, 2611
BseRI	GAGGAG	2 cut(s)	1391, 1547
BseX3I	CGGCCG	1 cut(s)	303
BseXI	GCAGC	9 cut(s)	202, 230, 233, 1017, 1395, 1552, 1558, 2192, 2459
BseYI	CCCAGC	2 cut(s)	475, 1827
Bsh1285I	CGRYCG	1 cut(s)	306
BshVI	ATCGAT	1 cut(s)	2496
BsiEI	CGRYCG	1 cut(s)	306
BsiHKAI	GWGCWC	4 cut(s)	168, 358, 438, 2064
BsiHKCI	CYCGRG	1 cut(s)	431
BsiSI	CCGG	4 cut(s)	306, 360, 1705, 1755
BsiWI	CGTACG	1 cut(s)	705
BslFI	GGGAC	5 cut(s)	331, 632, 1435, 1484, 1721
BsmAI	GTCTC	2 cut(s)	1906, 2635
BsmFI	GGGAC	5 cut(s)	331, 632, 1435, 1484, 1721
BsoBI	CYCGRG	1 cut(s)	431
Bsp1286I	GDGCHC	4 cut(s)	168, 358, 438, 2064
Bsp143I	GATC	4 cut(s)	372, 596, 1324, 1641
Bsp1720I	GCTNAGC	1 cut(s)	1448
Bsp19I	CCATGG	3 cut(s)	349, 1797, 2099
BspACI	CCGC	7 cut(s)	685, 785, 823, 1177, 1697, 2065, 2227
BspCNI	CTCAG	7 cut(s)	535, 1064, 1344, 1902, 2248, 2341, 2410
BspDI	ATCGAT	1 cut(s)	2496
BspHI	TCATGA	1 cut(s)	1330
BspLI	GGNNCC	2 cut(s)	672, 1472
BspMI	ACCTGC	2 cut(s)	534, 1861
BspOI	GCTAGC	1 cut(s)	1151
BspPI	GGATC	1 cut(s)	604
BspTI	CTTAAG	1 cut(s)	1034
BsrBI	CCGCTC	1 cut(s)	823
BsrDI	GCAATG	1 cut(s)	1525
BsrFI	RCCGGY	2 cut(s)	305, 1754
BsrI	ACTGG	5 cut(s)	742, 1196, 1610, 2054, 2611
BssAI	RCCGGY	2 cut(s)	305, 1754
BssMI	GATC	4 cut(s)	372, 596, 1324, 1641
BssNAI	GTATAC	1 cut(s)	472
BssT1I	CCWWGG	7 cut(s)	330, 349, 1022, 1797, 2026, 2099, 2344
Bst1107I	GTATAC	1 cut(s)	472
Bst2UI	CCWGG	7 cut(s)	269, 466, 1509, 1730, 1735, 1785, 2262
Bst4CI	ACNGT	9 cut(s)	440, 1238, 1285, 1475, 1659, 1777, 2081, 2236, 2627
Bst6I	CTCTTC	3 cut(s)	721, 1245, 1284
BstAFI	CTTAAG	1 cut(s)	1034
BstAPI	GCANNNNNTGC	2 cut(s)	1853, 2453
BstBAI	YACGTR	1 cut(s)	835
BstC8I	GCNNGC	7 cut(s)	605, 685, 1061, 1149, 1551, 1845, 2212
BstDSI	CCRYGG	4 cut(s)	349, 1797, 2077, 2099
BstENI	CCTNNNNNAGG	1 cut(s)	2259
BstF5I	GGATG	5 cut(s)	208, 346, 1017, 1459, 1693
BstH2I	RGCGCY	1 cut(s)	366
BstHHI	GCGC	2 cut(s)	365, 1017
BstKTI	GATC	4 cut(s)	375, 599, 1327, 1644
BstMAI	GTCTC	2 cut(s)	1906, 2635
BstMBI	GATC	4 cut(s)	372, 596, 1324, 1641
BstMCI	CGRYCG	1 cut(s)	306
BstNI	CCWGG	7 cut(s)	269, 466, 1509, 1730, 1735, 1785, 2262
BstNSI	RCATGY	1 cut(s)	1622
BstPAI	GACNNNNGTC	1 cut(s)	669
BstSCI	CCNGG	9 cut(s)	267, 358, 464, 1507, 1704, 1728, 1733, 1783, 2260
BstSFI	CTRYAG	1 cut(s)	2541
BstV1I	GCAGC	9 cut(s)	202, 230, 233, 1017, 1395, 1552, 1558, 2192, 2459
BstV2I	GAAGAC	3 cut(s)	1422, 1771, 1932
BstXI	CCANNNNNNTGG	1 cut(s)	331
BstZ17I	GTATAC	1 cut(s)	472
BstZI	CGGCCG	1 cut(s)	303
Bsu15I	ATCGAT	1 cut(s)	2496
Bsu36I	CCTNAGG	1 cut(s)	1352
BsuI	GTATCC	1 cut(s)	723
BsuTUI	ATCGAT	1 cut(s)	2496
BtgI	CCRYGG	4 cut(s)	349, 1797, 2077, 2099
BtrI	CACGTC	1 cut(s)	2548
BtsCI	GGATG	5 cut(s)	208, 346, 1017, 1459, 1693
BtsI	GCAGTG	1 cut(s)	2265
BtsIMutI	CAGTG	5 cut(s)	995, 1281, 1603, 2265, 2632
BveI	ACCTGC	2 cut(s)	534, 1861
Cac8I	GCNNGC	7 cut(s)	605, 685, 1061, 1149, 1551, 1845, 2212
CaiI	CAGNNNCTG	3 cut(s)	1605, 2267, 2435
CciI	TCATGA	1 cut(s)	1330
CfoI	GCGC	2 cut(s)	365, 1017
Cfr10I	RCCGGY	2 cut(s)	305, 1754
Cfr13I	GGNCC	5 cut(s)	671, 989, 1471, 2023, 2537
ClaI	ATCGAT	1 cut(s)	2496
Csp6I	GTAC	6 cut(s)	493, 706, 1255, 1667, 2002, 2145
CspCI	CAANNNNNGTGG	2 cut(s)	1738, 1773
CviQI	GTAC	6 cut(s)	493, 706, 1255, 1667, 2002, 2145
DpnI	GATC	4 cut(s)	374, 598, 1326, 1643
DpnII	GATC	4 cut(s)	372, 596, 1324, 1641
DrdI	GACNNNNNNGTC	1 cut(s)	2021
DseDI	GACNNNNNNGTC	1 cut(s)	2021
EaeI	YGGCCR	3 cut(s)	303, 829, 1731
EagI	CGGCCG	1 cut(s)	303
Eam1104I	CTCTTC	3 cut(s)	721, 1245, 1284
EarI	CTCTTC	3 cut(s)	721, 1245, 1284
Ecl136II	GAGCTC	3 cut(s)	166, 356, 2062
EclXI	CGGCCG	1 cut(s)	303
Eco130I	CCWWGG	7 cut(s)	330, 349, 1022, 1797, 2026, 2099, 2344
Eco147I	AGGCCT	1 cut(s)	521
Eco24I	GRGCYC	3 cut(s)	168, 358, 2064
Eco47I	GGWCC	3 cut(s)	671, 1471, 2023
Eco52I	CGGCCG	1 cut(s)	303
Eco53kI	GAGCTC	3 cut(s)	166, 356, 2062
Eco57I	CTGAAG	4 cut(s)	234, 1019, 2412, 2457
Eco72I	CACGTG	1 cut(s)	835
Eco81I	CCTNAGG	1 cut(s)	1352
Eco88I	CYCGRG	1 cut(s)	431
EcoICRI	GAGCTC	3 cut(s)	166, 356, 2062
EcoNI	CCTNNNNNAGG	1 cut(s)	2259
EcoRI	GAATTC	1 cut(s)	2616
EcoRII	CCWGG	7 cut(s)	267, 464, 1507, 1728, 1733, 1783, 2260
EcoT14I	CCWWGG	7 cut(s)	330, 349, 1022, 1797, 2026, 2099, 2344
EcoT22I	ATGCAT	1 cut(s)	1339
EcoT38I	GRGCYC	3 cut(s)	168, 358, 2064
ErhI	CCWWGG	7 cut(s)	330, 349, 1022, 1797, 2026, 2099, 2344
FaqI	GGGAC	5 cut(s)	331, 632, 1435, 1484, 1721
FauI	CCCGC	2 cut(s)	692, 1184
FauNDI	CATATG	1 cut(s)	2156
FbaI	TGATCA	2 cut(s)	372, 1641
FblI	GTMKAC	2 cut(s)	471, 2544
FokI	GGATG	5 cut(s)	195, 353, 1004, 1446, 1680
FriOI	GRGCYC	3 cut(s)	168, 358, 2064
FspBI	CTAG	8 cut(s)	315, 797, 875, 1148, 1208, 1952, 2399, 2444
GlaI	GCGC	2 cut(s)	364, 1016
GsaI	CCCAGC	2 cut(s)	479, 1831
GsuI	CTGGAG	2 cut(s)	290, 2027
HaeII	RGCGCY	1 cut(s)	366
HapII	CCGG	4 cut(s)	306, 360, 1705, 1755
HhaI	GCGC	2 cut(s)	365, 1017
Hin6I	GCGC	2 cut(s)	363, 1015
HinP1I	GCGC	2 cut(s)	363, 1015
HincII	GTYRAC	2 cut(s)	443, 1168
HindII	GTYRAC	2 cut(s)	443, 1168
HindIII	AAGCTT	5 cut(s)	138, 248, 1450, 1557, 1685
HpaI	GTTAAC	1 cut(s)	1168
HpaII	CCGG	4 cut(s)	306, 360, 1705, 1755
HphI	GGTGA	8 cut(s)	76, 320, 377, 1165, 1664, 2042, 2093, 2558
Hpy99I	CGWCG	3 cut(s)	47, 950, 2247
HpyAV	CCTTC	2 cut(s)	13, 25
HpyCH4III	ACNGT	9 cut(s)	440, 1238, 1285, 1475, 1659, 1777, 2081, 2236, 2627
HpyCH4IV	ACGT	6 cut(s)	834, 948, 1382, 1424, 2245, 2547
HpySE526I	ACGT	6 cut(s)	834, 948, 1382, 1424, 2245, 2547
HspAI	GCGC	2 cut(s)	363, 1015
Ksp22I	TGATCA	2 cut(s)	372, 1641
KspAI	GTTAAC	1 cut(s)	1168
Kzo9I	GATC	4 cut(s)	372, 596, 1324, 1641
LmnI	GCTCC	6 cut(s)	72, 353, 361, 1012, 1027, 2067
Lsp1109I	GCAGC	9 cut(s)	202, 230, 233, 1017, 1395, 1552, 1558, 2192, 2459
LweI	GCATC	7 cut(s)	1026, 1324, 1536, 1702, 2103, 2422, 2488
MaeI	CTAG	8 cut(s)	315, 797, 875, 1148, 1208, 1952, 2399, 2444
MaeII	ACGT	6 cut(s)	834, 948, 1382, 1424, 2245, 2547
MaeIII	GTNAC	3 cut(s)	485, 978, 2081
MalI	GATC	4 cut(s)	374, 598, 1326, 1643
MbiI	CCGCTC	1 cut(s)	823
MboI	GATC	4 cut(s)	372, 596, 1324, 1641
MfeI	CAATTG	1 cut(s)	1440
MhlI	GDGCHC	4 cut(s)	168, 358, 438, 2064
MlsI	TGGCCA	2 cut(s)	831, 1733
MluCI	AATT	9 cut(s)	395, 460, 766, 1130, 1440, 1956, 2188, 2319, 2616
MluNI	TGGCCA	2 cut(s)	831, 1733
MlyI	GAGTC	3 cut(s)	353, 667, 1544
MmeI	TCCRAC	1 cut(s)	99
Mox20I	TGGCCA	2 cut(s)	831, 1733
Mph1103I	ATGCAT	1 cut(s)	1339
MroXI	GAANNNNTTC	2 cut(s)	426, 1044
MscI	TGGCCA	2 cut(s)	831, 1733
MseI	TTAA	8 cut(s)	954, 1035, 1095, 1167, 1340, 1610, 1983, 2313
MslI	CAYNNNNRTG	3 cut(s)	1332, 1802, 2159
Msp20I	TGGCCA	2 cut(s)	831, 1733
MspCI	CTTAAG	1 cut(s)	1034
MspI	CCGG	4 cut(s)	306, 360, 1705, 1755
MspR9I	CCNGG	9 cut(s)	269, 360, 466, 1509, 1706, 1730, 1735, 1785, 2262
MunI	CAATTG	1 cut(s)	1440
MvaI	CCWGG	7 cut(s)	269, 466, 1509, 1730, 1735, 1785, 2262
NciI	CCSGG	2 cut(s)	360, 1706
NcoI	CCATGG	3 cut(s)	349, 1797, 2099
NdeI	CATATG	1 cut(s)	2156
NdeII	GATC	4 cut(s)	372, 596, 1324, 1641
NheI	GCTAGC	1 cut(s)	1147
NlaIV	GGNNCC	2 cut(s)	672, 1472
NmuCI	GTSAC	1 cut(s)	2081
NsiI	ATGCAT	1 cut(s)	1339
NspI	RCATGY	1 cut(s)	1622
PagI	TCATGA	1 cut(s)	1330
PceI	AGGCCT	1 cut(s)	521
PdmI	GAANNNNTTC	2 cut(s)	426, 1044
Pfl23II	CGTACG	1 cut(s)	705
PflFI	GACNNNGTC	1 cut(s)	623
PfoI	TCCNGGA	1 cut(s)	1704
PleI	GAGTC	3 cut(s)	352, 666, 1543
PmaCI	CACGTG	1 cut(s)	835
PmlI	CACGTG	1 cut(s)	835
PpsI	GAGTC	3 cut(s)	352, 666, 1543
Ppu21I	YACGTR	1 cut(s)	835
PshAI	GACNNNNGTC	1 cut(s)	669
PshBI	ATTAAT	1 cut(s)	1340
PsiI	TTATAA	1 cut(s)	1821
Psp124BI	GAGCTC	3 cut(s)	168, 358, 2064
Psp6I	CCWGG	7 cut(s)	267, 464, 1507, 1728, 1733, 1783, 2260
PspCI	CACGTG	1 cut(s)	835
PspFI	CCCAGC	2 cut(s)	475, 1827
PspGI	CCWGG	7 cut(s)	267, 464, 1507, 1728, 1733, 1783, 2260
PspLI	CGTACG	1 cut(s)	705
PspN4I	GGNNCC	2 cut(s)	672, 1472
PspPI	GGNCC	5 cut(s)	671, 989, 1471, 2023, 2537
PstNI	CAGNNNCTG	3 cut(s)	1605, 2267, 2435
PsyI	GACNNNGTC	1 cut(s)	623
RsaI	GTAC	6 cut(s)	494, 707, 1256, 1668, 2003, 2146
RsaNI	GTAC	6 cut(s)	493, 706, 1255, 1667, 2002, 2145
RseI	CAYNNNNRTG	3 cut(s)	1332, 1802, 2159
SacI	GAGCTC	3 cut(s)	168, 358, 2064
SaqAI	TTAA	8 cut(s)	954, 1035, 1095, 1167, 1340, 1610, 1983, 2313
Sau3AI	GATC	4 cut(s)	372, 596, 1324, 1641
Sau96I	GGNCC	5 cut(s)	671, 989, 1471, 2023, 2537
SchI	GAGTC	3 cut(s)	353, 667, 1544
ScrFI	CCNGG	9 cut(s)	269, 360, 466, 1509, 1706, 1730, 1735, 1785, 2262
SduI	GDGCHC	4 cut(s)	168, 358, 438, 2064
SfaNI	GCATC	7 cut(s)	1026, 1324, 1536, 1702, 2103, 2422, 2488
SfcI	CTRYAG	1 cut(s)	2541
SinI	GGWCC	3 cut(s)	671, 1471, 2023
SmiMI	CAYNNNNRTG	3 cut(s)	1332, 1802, 2159
SmlI	CTYRAG	6 cut(s)	404, 899, 1034, 1070, 1766, 1865
SmoI	CTYRAG	6 cut(s)	404, 899, 1034, 1070, 1766, 1865
SpeI	ACTAGT	1 cut(s)	314
Sse9I	AATT	9 cut(s)	395, 460, 766, 1130, 1440, 1956, 2188, 2319, 2616
SseBI	AGGCCT	1 cut(s)	521
SsiI	CCGC	7 cut(s)	685, 785, 823, 1177, 1697, 2065, 2227
SspI	AATATT	2 cut(s)	885, 2276
SspMI	CTAG	8 cut(s)	315, 797, 875, 1148, 1208, 1952, 2399, 2444
SstI	GAGCTC	3 cut(s)	168, 358, 2064
StuI	AGGCCT	1 cut(s)	521
StyD4I	CCNGG	9 cut(s)	267, 358, 464, 1507, 1704, 1728, 1733, 1783, 2260
StyI	CCWWGG	7 cut(s)	330, 349, 1022, 1797, 2026, 2099, 2344
TaaI	ACNGT	9 cut(s)	440, 1238, 1285, 1475, 1659, 1777, 2081, 2236, 2627
TaiI	ACGT	6 cut(s)	837, 951, 1385, 1427, 2248, 2550
TaqI	TCGA	8 cut(s)	742, 1241, 1530, 1789, 2136, 2381, 2484, 2496
TasI	AATT	9 cut(s)	395, 460, 766, 1130, 1440, 1956, 2188, 2319, 2616
TatI	WGTACW	4 cut(s)	492, 1254, 1666, 2001
TauI	GCSGC	1 cut(s)	787
Tru1I	TTAA	8 cut(s)	954, 1035, 1095, 1167, 1340, 1610, 1983, 2313
Tru9I	TTAA	8 cut(s)	954, 1035, 1095, 1167, 1340, 1610, 1983, 2313
TscAI	CASTG	5 cut(s)	1002, 1288, 1610, 2272, 2632
TseFI	GTSAC	1 cut(s)	2081
TseI	GCWGC	9 cut(s)	190, 218, 221, 1030, 1408, 1546, 1565, 2180, 2447
Tsp45I	GTSAC	1 cut(s)	2081
TspDTI	ATGAA	8 cut(s)	119, 1286, 1306, 1536, 1730, 2001, 2109, 2239
TspGWI	ACGGA	1 cut(s)	1385
TspRI	CASTG	5 cut(s)	1002, 1288, 1610, 2272, 2632
Tth111I	GACNNNGTC	1 cut(s)	623
Vha464I	CTTAAG	1 cut(s)	1034
VpaK11BI	GGWCC	3 cut(s)	671, 1471, 2023
VspI	ATTAAT	1 cut(s)	1340
XagI	CCTNNNNNAGG	1 cut(s)	2259
XapI	RAATTY	3 cut(s)	1130, 1956, 2616
XbaI	TCTAGA	1 cut(s)	796
XceI	RCATGY	1 cut(s)	1622
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2342
XmiI	GTMKAC	2 cut(s)	471, 2544
XmnI	GAANNNNTTC	2 cut(s)	426, 1044
XspI	CTAG	8 cut(s)	315, 797, 875, 1148, 1208, 1952, 2399, 2444
Zsp2I	ATGCAT	1 cut(s)	1339

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.