Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0009911

Physical Location & Seq

Genomic Coordinates Forward (+)

18085851 .. 18090573

Gene Structure 4723 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1201500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1398 bp

ATGGCGTCCATGGCCACCCTTTTCGTCAACACCCCCTACCACTCTCACTCTTTCCCCAAGCCGTCATCTGCCCATTTCGCCCCCGTCCTCAAGCTCTCAACCCATCTCGGGTCCAAGCCCAGGAACCGCCAAAGGCTCCGAATTTCAAACGGCTTGATCGAGCCGGACGGCGGGAAGCTGGCGGAGCTCTTCGTCGAGGAGTCGAAGAAAGGCGAGATGAAGAAAAAGGCGGCGGCGCTGCCGAGGATCAAGCTTTCAAGGATCGACCTCCAATGGGTTCACGTGATCAGCGAAGGCTGGTCGAGCCCTCTCCGCGGATTCATGAGAGAATCCGAGTTCCTCCAAACTCTTCATTTCAATTCGCTCCGACTCGATGACGGGTCGGTCGTGAATATGTCGGTGCCGATAGTCCTCGCCATCGACGACGCGCAGAAACGCCGTATCGGCCAGTCGACCCGGGTCGCGCTCGTCGACTCGGATGATAATCTGATCGCTATTTTGAAGGATATTGAGATCTTTAAGCACCATAAAGAAGAACGTATTGCCCGCACTTGGGGAACAACTGCCCCTGGTTTGCCTTATGTTGATCAAGCCATAACCAATGCTGGAAACTGGCTGATTGGAGGTGACCTGGAGGTTCTAGAACCTATAAAGTACAACGATGGCCTTGATCGCTTCCGACTGTCTCCTGCACAACTCCGTGATGAATTTACAAAGCGCAATGCTGATGCTGTATTTGCGTTTCAGCTGCGAAATCCTGTTCACAATGGACATGCTTTGCTGATGACCGATACCCGTCGTCGGCTTCTTGAGATGGGATACAAGAACCCCATTCTCTTGCTTCATCCATTGGGAGGCTATACAAAGGCAGATGACGTTCCACTTAGTTGGCGAATGAAACAACATGAGAAGGTTCTTGAGGATGGTGTTCTTGATCCTGAGACGACTGTGGTTTCCATATTCCCCTCTCCCATGCACTATGCTGGCCCAACTGAGGTGCAGTGGCACGCAAAGGCTAGGATTAATGCAGGTGCTAACTTTTACATTGTTGGTCGGGACCCTGCAGGGATGGATCATCCAACAGAGAAGAGGGATCTGTATGATGCCGATCATGGCAAGAAAGTATTGAGTATGGCACCTGGACTGGAGAGACTTAACATCCTTCCCTTCAAGGTTGCTGCTTATGATAAAACTCAAGGTAAAATGGCATTTTTTGATCCCTCAAGGCCTCAGGACTTCCTTTTCATATCCGGCACAAAGATGCGAACGCTTGCAAGGAACAAAGAGAACCCTCCCGAAGGATTCATGTGCCCTAATGGCTGGAAGGTGCTTGTCGACTACTATGATAGTTTGGTTCCTGCGGACAACGACAAAGTCCCAGAAGCAGTTCCAGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000103 GO:0001887 GO:0003674 GO:0003824 GO:0004020 GO:0004779 GO:0004781 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005886 GO:0006790 GO:0006793 GO:0006796 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0009267 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009719 GO:0009725 GO:0009735 GO:0009970 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010038 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016779 GO:0022607 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0042221 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0050896 GO:0051259 GO:0051716 GO:0065003 GO:0070206 GO:0070566 GO:0071496 GO:0071840 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00230 ko00261 ko00450 ko00920 ko01100 ko01120 ko01130 map00230 map00261 map00450 map00920 map01100 map01120 map01130

Pfam Domains

Protein Families

ATP-sulfurylase PUA_2

Protein Analysis

466

Amino Acids

52.28

Weight (kDa)

8.82

Isoelectric Point (pI)

48.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PUA_2	PF14306	53 - 215	6.8e-46	PUA-like domain
ATP-sulfurylase	PF01747	225 - 446	1e-66	ATP-sulfurylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009911)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G22890 AT4G14680 AT4G14680 AT5G43780
fragaria_vesca	FvH4_4g01660
malus_domestica	MD13G1201500.v1.1 MD16G1201400.v1.1
prunus_persica	Prupe.1G023000_v2.0.a1
pyrus_communis	pycom13g17480 pycom16g17000
rosa_chinensis	RchiOBHm_Chr4g0388281
rosa_laevigata	RLG00000010068
rosa_multiflora	Rmu_sc0000753.1_g000031 Rmu_sc0001949.1_g000019
rosa_roxburghii	Rroxscaffold_5G00334740
rosa_rugosa	Rorug03G0316600
rosa_samantha	Rh4AG022000 Rh4BG015900 Rh4CG022500 Rh4DG016800
rosa_wichuraiana	Rw4G001450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1019
AasI	GACNNNNNNGTC	1 cut(s)	383
Acc36I	ACCTGC	1 cut(s)	1019
AccB1I	GGYRCC	2 cut(s)	400, 1135
AccI	GTMKAC	3 cut(s)	452, 471, 1335
AccII	CGCG	3 cut(s)	315, 428, 464
AciI	CCGC	9 cut(s)	127, 171, 182, 230, 233, 313, 315, 547, 1361
AclWI	GGATC	6 cut(s)	254, 269, 929, 1080, 1101, 1211
AcoI	YGGCCR	2 cut(s)	12, 445
AcsI	RAATTY	2 cut(s)	141, 707
AcvI	CACGTG	1 cut(s)	283
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	1 cut(s)	656
AgsI	TTSAA	5 cut(s)	147, 258, 358, 502, 1171
AjnI	CCWGG	4 cut(s)	119, 568, 630, 1138
AluBI	AGCT	6 cut(s)	94, 178, 187, 253, 748, 1394
AluI	AGCT	6 cut(s)	94, 178, 187, 253, 748, 1394
Alw21I	GWGCWC	1 cut(s)	189
Alw26I	GTCTC	3 cut(s)	690, 935, 1144
AlwI	GGATC	6 cut(s)	254, 269, 929, 1080, 1101, 1211
Ama87I	CYCGRG	2 cut(s)	107, 456
AoxI	GGCC	5 cut(s)	12, 445, 664, 985, 1226
ApeKI	GCWGC	3 cut(s)	238, 748, 1178
ApoI	RAATTY	2 cut(s)	141, 707
AseI	ATTAAT	1 cut(s)	1023
Asp700I	GAANNNNTTC	1 cut(s)	1386
AspLEI	GCGC	4 cut(s)	238, 430, 466, 720
AspS9I	GGNCC	3 cut(s)	111, 986, 1057
AsuC2I	CCSGG	2 cut(s)	457, 458
AsuHPI	GGTGA	1 cut(s)	638
AvaI	CYCGRG	2 cut(s)	107, 456
AvaII	GGWCC	2 cut(s)	111, 1057
AxyI	CCTNAGG	1 cut(s)	1230
BaeGI	GKGCMC	1 cut(s)	1313
BalI	TGGCCA	1 cut(s)	14
BanI	GGYRCC	2 cut(s)	400, 1135
BanII	GRGCYC	2 cut(s)	189, 308
BbrPI	CACGTG	1 cut(s)	283
Bbv12I	GWGCWC	1 cut(s)	189
BbvI	GCAGC	3 cut(s)	225, 735, 1165
BccI	CCATC	6 cut(s)	111, 425, 656, 808, 917, 1063
BceAI	ACGGC	4 cut(s)	46, 166, 184, 423
BciT130I	CCWGG	4 cut(s)	121, 570, 632, 1140
BciVI	GTATCC	1 cut(s)	812
BclI	TGATCA	2 cut(s)	285, 586
BcnI	CCSGG	2 cut(s)	457, 458
BcoDI	GTCTC	3 cut(s)	690, 935, 1144
BfaI	CTAG	2 cut(s)	641, 1017
BfmI	CTRYAG	1 cut(s)	1062
BfoI	RGCGCY	1 cut(s)	239
BfuAI	ACCTGC	1 cut(s)	1019
BfuI	GTATCC	1 cut(s)	812
BglI	GCCNNNNNGGC	2 cut(s)	444, 1317
BglII	AGATCT	1 cut(s)	513
BisI	GCNGC	5 cut(s)	231, 234, 239, 749, 1179
BlsI	GCNGC	5 cut(s)	232, 235, 240, 750, 1180
Bme1390I	CCNGG	6 cut(s)	121, 457, 458, 570, 632, 1140
Bme18I	GGWCC	2 cut(s)	111, 1057
BmeT110I	CYCGRG	2 cut(s)	107, 456
BmgT120I	GGNCC	3 cut(s)	111, 986, 1057
BmiI	GGNNCC	8 cut(s)	112, 125, 137, 402, 1058, 1059, 1137, 1356
BmrFI	CCNGG	6 cut(s)	121, 457, 458, 570, 632, 1140
BmsI	GCATC	3 cut(s)	718, 1093, 1251
BoxI	GACNNNNGTC	1 cut(s)	458
BpmI	CTGGAG	2 cut(s)	653, 1166
BpuEI	CTTGAG	5 cut(s)	74, 830, 938, 1179, 1207
BpuMI	CCSGG	2 cut(s)	457, 458
BsaAI	YACGTR	1 cut(s)	283
BsaBI	GATNNNNATC	2 cut(s)	483, 1107
BsaHI	GRCGYC	1 cut(s)	5
BsaJI	CCNNGG	6 cut(s)	9, 119, 242, 313, 456, 568
Bse1I	ACTGG	3 cut(s)	448, 617, 1149
Bse21I	CCTNAGG	1 cut(s)	1230
Bse3DI	GCAATG	1 cut(s)	727
Bse8I	GATNNNNATC	2 cut(s)	483, 1107
BseBI	CCWGG	4 cut(s)	121, 570, 632, 1140
BseDI	CCNNGG	6 cut(s)	9, 119, 242, 313, 456, 568
BseGI	GGATG	6 cut(s)	484, 844, 928, 1074, 1075, 1158
BseJI	GATNNNNATC	2 cut(s)	483, 1107
BseMI	GCAATG	1 cut(s)	727
BseMII	CTCAG	3 cut(s)	930, 984, 1244
BseNI	ACTGG	3 cut(s)	448, 617, 1149
BseRI	GAGGAG	1 cut(s)	212
BseSI	GKGCMC	1 cut(s)	1313
BseXI	GCAGC	3 cut(s)	225, 735, 1165
BsgI	GTGCAG	2 cut(s)	675, 1019
Bsh1236I	CGCG	3 cut(s)	315, 428, 464
Bsh1285I	CGRYCG	1 cut(s)	387
BshFI	GGCC	5 cut(s)	14, 447, 666, 987, 1228
BshNI	GGYRCC	2 cut(s)	400, 1135
BsiEI	CGRYCG	1 cut(s)	387
BsiHKAI	GWGCWC	1 cut(s)	189
BsiHKCI	CYCGRG	2 cut(s)	107, 456
BsiSI	CCGG	3 cut(s)	164, 457, 1251
BslFI	GGGAC	2 cut(s)	1070, 1361
BsmAI	GTCTC	3 cut(s)	690, 935, 1144
BsmBI	CGTCTC	1 cut(s)	935
BsmFI	GGGAC	2 cut(s)	1070, 1361
BsnI	GGCC	5 cut(s)	14, 447, 666, 987, 1228
BsoBI	CYCGRG	2 cut(s)	107, 456
Bsp1286I	GDGCHC	3 cut(s)	189, 308, 1313
Bsp19I	CCATGG	1 cut(s)	9
BspACI	CCGC	9 cut(s)	127, 171, 182, 230, 233, 313, 315, 547, 1361
BspANI	GGCC	5 cut(s)	14, 447, 666, 987, 1228
BspCNI	CTCAG	3 cut(s)	931, 985, 1243
BspFNI	CGCG	3 cut(s)	315, 428, 464
BspHI	TCATGA	1 cut(s)	321
BspLI	GGNNCC	8 cut(s)	112, 125, 137, 402, 1058, 1059, 1137, 1356
BspMAI	CTGCAG	1 cut(s)	1066
BspMI	ACCTGC	1 cut(s)	1019
BspPI	GGATC	6 cut(s)	254, 269, 929, 1080, 1101, 1211
BspQI	GCTCTTC	1 cut(s)	194
BspT107I	GGYRCC	2 cut(s)	400, 1135
BsrDI	GCAATG	1 cut(s)	727
BsrI	ACTGG	3 cut(s)	448, 617, 1149
BssECI	CCNNGG	6 cut(s)	9, 119, 242, 313, 456, 568
BssNI	GRCGYC	1 cut(s)	5
BssT1I	CCWWGG	1 cut(s)	9
Bst2UI	CCWGG	4 cut(s)	121, 570, 632, 1140
Bst4CI	ACNGT	2 cut(s)	684, 949
Bst6I	CTCTTC	3 cut(s)	194, 354, 1082
BstACI	GRCGYC	1 cut(s)	5
BstBAI	YACGTR	1 cut(s)	283
BstC8I	GCNNGC	5 cut(s)	180, 547, 985, 1008, 1272
BstDEI	CTNAG	5 cut(s)	884, 939, 993, 1230, 1395
BstDSI	CCRYGG	2 cut(s)	9, 313
BstEII	GGTNACC	1 cut(s)	626
BstENI	CCTNNNNNAGG	1 cut(s)	1296
BstF5I	GGATG	6 cut(s)	484, 844, 928, 1074, 1075, 1158
BstFNI	CGCG	3 cut(s)	315, 428, 464
BstH2I	RGCGCY	1 cut(s)	239
BstHHI	GCGC	4 cut(s)	238, 430, 466, 720
BstMAI	GTCTC	3 cut(s)	690, 935, 1144
BstMCI	CGRYCG	1 cut(s)	387
BstNI	CCWGG	4 cut(s)	121, 570, 632, 1140
BstNSI	RCATGY	1 cut(s)	776
BstPAI	GACNNNNGTC	1 cut(s)	458
BstPI	GGTNACC	1 cut(s)	626
BstSCI	CCNGG	6 cut(s)	119, 455, 456, 568, 630, 1138
BstSFI	CTRYAG	1 cut(s)	1062
BstSLI	GKGCMC	1 cut(s)	1313
BstUI	CGCG	3 cut(s)	315, 428, 464
BstV1I	GCAGC	3 cut(s)	225, 735, 1165
BstX2I	RGATCY	2 cut(s)	513, 1093
BstXI	CCANNNNNNTGG	1 cut(s)	888
BstYI	RGATCY	2 cut(s)	513, 1093
Bsu36I	CCTNAGG	1 cut(s)	1230
BsuI	GTATCC	1 cut(s)	812
BsuRI	GGCC	5 cut(s)	14, 447, 666, 987, 1228
BtgI	CCRYGG	2 cut(s)	9, 313
BtsCI	GGATG	6 cut(s)	484, 844, 928, 1074, 1075, 1158
BtsI	GCAGTG	1 cut(s)	1007
BtsIMutI	CAGTG	1 cut(s)	1007
BveI	ACCTGC	1 cut(s)	1019
Cac8I	GCNNGC	5 cut(s)	180, 547, 985, 1008, 1272
CciI	TCATGA	1 cut(s)	321
CfoI	GCGC	4 cut(s)	238, 430, 466, 720
Cfr13I	GGNCC	3 cut(s)	111, 986, 1057
Cfr42I	CCGCGG	1 cut(s)	316
Cfr9I	CCCGGG	1 cut(s)	456
CseI	GACGC	1 cut(s)	434
Csp6I	GTAC	1 cut(s)	655
CviAII	CATG	7 cut(s)	10, 322, 773, 905, 973, 1112, 1306
CviQI	GTAC	1 cut(s)	655
DdeI	CTNAG	5 cut(s)	884, 939, 993, 1230, 1395
DrdI	GACNNNNNNGTC	1 cut(s)	383
DseDI	GACNNNNNNGTC	1 cut(s)	383
EaeI	YGGCCR	2 cut(s)	12, 445
Eam1104I	CTCTTC	3 cut(s)	194, 354, 1082
EarI	CTCTTC	3 cut(s)	194, 354, 1082
EciI	GGCGGA	1 cut(s)	197
Ecl136II	GAGCTC	1 cut(s)	187
Eco130I	CCWWGG	1 cut(s)	9
Eco147I	AGGCCT	1 cut(s)	1228
Eco24I	GRGCYC	2 cut(s)	189, 308
Eco47I	GGWCC	2 cut(s)	111, 1057
Eco53kI	GAGCTC	1 cut(s)	187
Eco72I	CACGTG	1 cut(s)	283
Eco81I	CCTNAGG	1 cut(s)	1230
Eco88I	CYCGRG	2 cut(s)	107, 456
Eco91I	GGTNACC	1 cut(s)	626
EcoICRI	GAGCTC	1 cut(s)	187
EcoNI	CCTNNNNNAGG	1 cut(s)	1296
EcoO109I	RGGNCCY	1 cut(s)	1057
EcoO65I	GGTNACC	1 cut(s)	626
EcoRII	CCWGG	4 cut(s)	119, 568, 630, 1138
EcoT14I	CCWWGG	1 cut(s)	9
EcoT38I	GRGCYC	2 cut(s)	189, 308
ErhI	CCWWGG	1 cut(s)	9
Esp3I	CGTCTC	1 cut(s)	935
FaeI	CATG	7 cut(s)	13, 325, 776, 908, 976, 1115, 1309
FaqI	GGGAC	2 cut(s)	1070, 1361
FatI	CATG	7 cut(s)	9, 321, 772, 904, 972, 1111, 1305
FauI	CCCGC	2 cut(s)	164, 554
FbaI	TGATCA	2 cut(s)	285, 586
FblI	GTMKAC	3 cut(s)	452, 471, 1335
Fnu4HI	GCNGC	5 cut(s)	231, 234, 239, 749, 1179
FokI	GGATG	6 cut(s)	491, 831, 935, 1062, 1081, 1145
FriOI	GRGCYC	2 cut(s)	189, 308
Fsp4HI	GCNGC	5 cut(s)	231, 234, 239, 749, 1179
FspBI	CTAG	2 cut(s)	641, 1017
GlaI	GCGC	4 cut(s)	237, 429, 465, 719
GluI	GCNGC	5 cut(s)	231, 234, 239, 749, 1179
GsuI	CTGGAG	2 cut(s)	653, 1166
HaeII	RGCGCY	1 cut(s)	239
HaeIII	GGCC	5 cut(s)	14, 447, 666, 987, 1228
HapII	CCGG	3 cut(s)	164, 457, 1251
HgaI	GACGC	1 cut(s)	434
HhaI	GCGC	4 cut(s)	238, 430, 466, 720
Hin1I	GRCGYC	1 cut(s)	5
Hin1II	CATG	7 cut(s)	13, 325, 776, 908, 976, 1115, 1309
Hin6I	GCGC	4 cut(s)	236, 428, 464, 718
HinP1I	GCGC	4 cut(s)	236, 428, 464, 718
HincII	GTYRAC	4 cut(s)	28, 453, 472, 1336
HindII	GTYRAC	4 cut(s)	28, 453, 472, 1336
HindIII	AAGCTT	1 cut(s)	251
HinfI	GANTC	6 cut(s)	200, 318, 329, 369, 473, 1302
HpaII	CCGG	3 cut(s)	164, 457, 1251
HphI	GGTGA	1 cut(s)	638
Hpy166II	GTNNAC	6 cut(s)	28, 280, 453, 472, 763, 1336
Hpy188I	TCNGA	6 cut(s)	140, 334, 368, 478, 489, 680
Hpy8I	GTNNAC	6 cut(s)	28, 280, 453, 472, 763, 1336
Hpy99I	CGWCG	6 cut(s)	197, 425, 428, 473, 801, 804
HpyAV	CCTTC	7 cut(s)	287, 496, 904, 1172, 1177, 1292, 1318
HpyCH4III	ACNGT	2 cut(s)	684, 949
HpyCH4IV	ACGT	3 cut(s)	282, 538, 876
HpyCH4V	TGCA	6 cut(s)	692, 976, 1000, 1028, 1064, 1274
HpyF3I	CTNAG	5 cut(s)	884, 939, 993, 1230, 1395
HpySE526I	ACGT	3 cut(s)	282, 538, 876
Hsp92I	GRCGYC	1 cut(s)	5
Hsp92II	CATG	7 cut(s)	13, 325, 776, 908, 976, 1115, 1309
HspAI	GCGC	4 cut(s)	236, 428, 464, 718
KflI	GGGWCCC	1 cut(s)	1057
Ksp22I	TGATCA	2 cut(s)	285, 586
KspI	CCGCGG	1 cut(s)	316
LguI	GCTCTTC	1 cut(s)	194
LmnI	GCTCC	3 cut(s)	141, 184, 369
Lsp1109I	GCAGC	3 cut(s)	225, 735, 1165
LweI	GCATC	3 cut(s)	718, 1093, 1251
MaeI	CTAG	2 cut(s)	641, 1017
MaeII	ACGT	3 cut(s)	282, 538, 876
MaeIII	GTNAC	1 cut(s)	626
MboII	GAAGA	6 cut(s)	181, 217, 232, 341, 545, 1099
MflI	RGATCY	2 cut(s)	513, 1093
MhlI	GDGCHC	3 cut(s)	189, 308, 1313
MlsI	TGGCCA	1 cut(s)	14
MluCI	AATT	3 cut(s)	141, 358, 707
MluNI	TGGCCA	1 cut(s)	14
MlyI	GAGTC	3 cut(s)	209, 363, 467
MmeI	TCCRAC	3 cut(s)	391, 703, 1103
Mox20I	TGGCCA	1 cut(s)	14
MroXI	GAANNNNTTC	1 cut(s)	1386
MscI	TGGCCA	1 cut(s)	14
MseI	TTAA	3 cut(s)	519, 1023, 1155
MslI	CAYNNNNRTG	1 cut(s)	1259
Msp20I	TGGCCA	1 cut(s)	14
MspA1I	CMGCKG	2 cut(s)	315, 748
MspI	CCGG	3 cut(s)	164, 457, 1251
MspR9I	CCNGG	6 cut(s)	121, 457, 458, 570, 632, 1140
MvaI	CCWGG	4 cut(s)	121, 570, 632, 1140
MvnI	CGCG	3 cut(s)	315, 428, 464
NciI	CCSGG	2 cut(s)	457, 458
NcoI	CCATGG	1 cut(s)	9
NlaIII	CATG	7 cut(s)	13, 325, 776, 908, 976, 1115, 1309
NlaIV	GGNNCC	8 cut(s)	112, 125, 137, 402, 1058, 1059, 1137, 1356
NmeAIII	GCCGAG	1 cut(s)	267
NmuCI	GTSAC	1 cut(s)	626
NspI	RCATGY	1 cut(s)	776
PagI	TCATGA	1 cut(s)	321
PaqCI	CACCTGC	1 cut(s)	1019
PceI	AGGCCT	1 cut(s)	1228
PciSI	GCTCTTC	1 cut(s)	194
PcsI	WCGNNNNNNNCGW	5 cut(s)	156, 288, 384, 420, 468
PdmI	GAANNNNTTC	1 cut(s)	1386
PfeI	GAWTC	3 cut(s)	318, 329, 1302
PflFI	GACNNNGTC	1 cut(s)	1373
PkrI	GCNGC	5 cut(s)	232, 235, 240, 750, 1180
PleI	GAGTC	3 cut(s)	208, 363, 467
PmaCI	CACGTG	1 cut(s)	283
PmlI	CACGTG	1 cut(s)	283
PpsI	GAGTC	3 cut(s)	208, 363, 467
Ppu21I	YACGTR	1 cut(s)	283
PpuMI	RGGWCCY	1 cut(s)	1057
PshAI	GACNNNNGTC	1 cut(s)	458
PshBI	ATTAAT	1 cut(s)	1023
Psp124BI	GAGCTC	1 cut(s)	189
Psp5II	RGGWCCY	1 cut(s)	1057
Psp6I	CCWGG	4 cut(s)	119, 568, 630, 1138
PspCI	CACGTG	1 cut(s)	283
PspEI	GGTNACC	1 cut(s)	626
PspGI	CCWGG	4 cut(s)	119, 568, 630, 1138
PspN4I	GGNNCC	8 cut(s)	112, 125, 137, 402, 1058, 1059, 1137, 1356
PspPI	GGNCC	3 cut(s)	111, 986, 1057
PspPPI	RGGWCCY	1 cut(s)	1057
PstI	CTGCAG	1 cut(s)	1066
PsuI	RGATCY	2 cut(s)	513, 1093
PsyI	GACNNNGTC	1 cut(s)	1373
PvuII	CAGCTG	1 cut(s)	748
RsaI	GTAC	1 cut(s)	656
RsaNI	GTAC	1 cut(s)	655
RseI	CAYNNNNRTG	1 cut(s)	1259
SacI	GAGCTC	1 cut(s)	189
SacII	CCGCGG	1 cut(s)	316
SalI	GTCGAC	3 cut(s)	451, 470, 1334
SapI	GCTCTTC	1 cut(s)	194
SaqAI	TTAA	3 cut(s)	519, 1023, 1155
SatI	GCNGC	5 cut(s)	231, 234, 239, 749, 1179
Sau96I	GGNCC	3 cut(s)	111, 986, 1057
SbfI	CCTGCAGG	1 cut(s)	1066
SchI	GAGTC	3 cut(s)	209, 363, 467
ScrFI	CCNGG	6 cut(s)	121, 457, 458, 570, 632, 1140
SdaI	CCTGCAGG	1 cut(s)	1066
SduI	GDGCHC	3 cut(s)	189, 308, 1313
SfaNI	GCATC	3 cut(s)	718, 1093, 1251
SfcI	CTRYAG	1 cut(s)	1062
Sfr303I	CCGCGG	1 cut(s)	316
SgrBI	CCGCGG	1 cut(s)	316
SinI	GGWCC	2 cut(s)	111, 1057
SmaI	CCCGGG	1 cut(s)	458
SmiMI	CAYNNNNRTG	1 cut(s)	1259
SmlI	CTYRAG	5 cut(s)	89, 809, 917, 1194, 1222
SmoI	CTYRAG	5 cut(s)	89, 809, 917, 1194, 1222
Sse8387I	CCTGCAGG	1 cut(s)	1066
Sse9I	AATT	3 cut(s)	141, 358, 707
SseBI	AGGCCT	1 cut(s)	1228
SsiI	CCGC	9 cut(s)	127, 171, 182, 230, 233, 313, 315, 547, 1361
SspMI	CTAG	2 cut(s)	641, 1017
SstI	GAGCTC	1 cut(s)	189
StuI	AGGCCT	1 cut(s)	1228
StyD4I	CCNGG	6 cut(s)	119, 455, 456, 568, 630, 1138
StyI	CCWWGG	1 cut(s)	9
TaaI	ACNGT	2 cut(s)	684, 949
TaiI	ACGT	3 cut(s)	285, 541, 879
TaqII	GACCGA	2 cut(s)	373, 803
TasI	AATT	3 cut(s)	141, 358, 707
TatI	WGTACW	1 cut(s)	654
TauI	GCSGC	2 cut(s)	233, 236
TfiI	GAWTC	3 cut(s)	318, 329, 1302
Tru1I	TTAA	3 cut(s)	519, 1023, 1155
Tru9I	TTAA	3 cut(s)	519, 1023, 1155
TscAI	CASTG	1 cut(s)	1007
TseFI	GTSAC	1 cut(s)	626
TseI	GCWGC	3 cut(s)	238, 748, 1178
Tsp45I	GTSAC	1 cut(s)	626
TspDTI	ATGAA	8 cut(s)	233, 310, 341, 720, 833, 911, 1234, 1294
TspGWI	ACGGA	1 cut(s)	689
TspMI	CCCGGG	1 cut(s)	456
TspRI	CASTG	1 cut(s)	1007
Tth111I	GACNNNGTC	1 cut(s)	1373
VpaK11BI	GGWCC	2 cut(s)	111, 1057
VspI	ATTAAT	1 cut(s)	1023
XagI	CCTNNNNNAGG	1 cut(s)	1296
XapI	RAATTY	2 cut(s)	141, 707
XbaI	TCTAGA	1 cut(s)	640
XceI	RCATGY	1 cut(s)	776
XmaI	CCCGGG	1 cut(s)	456
XmiI	GTMKAC	3 cut(s)	452, 471, 1335
XmnI	GAANNNNTTC	1 cut(s)	1386
XspI	CTAG	2 cut(s)	641, 1017

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.