Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0009911

Physical Location & Seq

Genomic Coordinates Forward (+)

18313416 .. 18318281

Gene Structure 4866 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1201400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1398 bp

ATGGCGTCCATGGCCACCCTTTTCGTCAAAACCCCCTACCCCTCTCACACTTTCCCCAAAACTTCAACTACCCATTTCTTCCCGGCCCTCAAGCTCTCAACCCATCTCGGGTCCAAGCCCAGGACCCCCCAGAGGCTCCGAATTTCAAACGGGTTGATCGAGCCGGACGGCGGGAAGCTCGTCGAGCTCTTCGTCCATGAGTCGAAGAAGGGCGAGACGAAGAAAGAGGCGGCGGCGCTGCCCAGGATCAAGCTTTCAAGGATCGACCTCCAATGGGTTCACGTGATCAGCGAAGGCTGGTCGAGCCCTCTCCGCGGATTCATGAGAGAATCCGAGTTCCTCCAAACTCTTCATTTCAATTCACTCCGACTCGATGACGGGTCGGTAGTGAATATGTCCGTGCCGATCGTTCTGGCCATCGACGACGCGCAGAAACGCCGGATCTGCGAATCGACCAGAGTCGCGCTTGTCGACTCGGATGATAATCTGATCGCTATTTTGAAAGACATTGAGATCTATAAGCACCATAAAGAAGAACGGATTGCCCGCACTTGGGGAACAACTGCCCCTGGTTTACCTTATGTTGATCAAGCCATAACCAATGCAGGAAATTGGCTGATTGGAGGTGACCTAGAGGTTCTAGAACCTATAAAGTACAATGATGGCCTTGATCGCTTCCGATTGTCTCCTGCACAACTCCGTGAAGAATTCACAAAGCGCAATGCTGACGCTGTATTTGCGTTTCAGCTGCGAAATCCTGTCCACAATGGACATGCTTTGTTGATGACTGATACCCGTCGTCGGCTTCTTGAGATGGGCTACAAGAACCCCATTCTGTTGCTTCATCCATTAGGAGGCTATACAAAGGCAGATGACGTTCCACTTAGTTGGCGAATGAAACAACATGAGAAGGTTCTTGAGGATGGTGTCCTTGATCCCGAGACGACTGTTGTTTCCATTTTTCCCTCTCCTATGCACTATGCTGGCCCAACTGAGGTGCAGTGGCATGCAAAGGCTAGGATCAATGCAGGGGCCGACTTTTACATTGTTGGTCGGGATCCTGCAGGTATGGGTCATCCAACTGAGAAGAGAGATCTGTATGATGCCGATCATGGCAAGAAAGTATTAAGCATGGCACCTGGACTGGAGCGACTTAACATCCTTCCCTTCAAGGTTGCTGCTTATGATAAAACTCAAGGCAAAATGGCATTTTTTGATCCCTCGAGGCCTCAGGACTTCCTTTTCATATCTGGCACAAAGATGCGAACACTTGCAAGGAACAAAGAGAACCCTCCCGAAGGATTCATGTGCCCTAGTGGCTGGGAGGTGCTAGTTGACTACTATGACAGTTTGGCTCCTGCGGACAATGGTAAAGTCCCAGAAGCTGTTCCAGCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000103 GO:0001887 GO:0003674 GO:0003824 GO:0004020 GO:0004779 GO:0004781 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005886 GO:0006790 GO:0006793 GO:0006796 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0009267 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009719 GO:0009725 GO:0009735 GO:0009970 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010038 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016779 GO:0022607 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0042221 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0050896 GO:0051259 GO:0051716 GO:0065003 GO:0070206 GO:0070566 GO:0071496 GO:0071840 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00230 ko00261 ko00450 ko00920 ko01100 ko01120 ko01130 map00230 map00261 map00450 map00920 map01100 map01120 map01130

Pfam Domains

Protein Families

ATP-sulfurylase PUA_2

Protein Analysis

466

Amino Acids

52.21

Weight (kDa)

7.81

Isoelectric Point (pI)

48.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PUA_2	PF14306	53 - 215	1.6e-46	PUA-like domain
ATP-sulfurylase	PF01747	225 - 446	2.4e-67	ATP-sulfurylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009911)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G22890 AT4G14680 AT4G14680 AT5G43780
fragaria_vesca	FvH4_4g01660
malus_domestica	MD13G1201500.v1.1 MD16G1201400.v1.1
prunus_persica	Prupe.1G023000_v2.0.a1
pyrus_communis	pycom13g17480 pycom16g17000
rosa_chinensis	RchiOBHm_Chr4g0388281
rosa_laevigata	RLG00000010068
rosa_multiflora	Rmu_sc0000753.1_g000031 Rmu_sc0001949.1_g000019
rosa_roxburghii	Rroxscaffold_5G00334740
rosa_rugosa	Rorug03G0316600
rosa_samantha	Rh4AG022000 Rh4BG015900 Rh4CG022500 Rh4DG016800
rosa_wichuraiana	Rw4G001450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	1222
Acc36I	ACCTGC	1 cut(s)	1055
AccB1I	GGYRCC	1 cut(s)	1135
AccI	GTMKAC	1 cut(s)	471
AccII	CGCG	3 cut(s)	315, 428, 464
AciI	CCGC	7 cut(s)	171, 230, 233, 313, 315, 547, 1361
AclWI	GGATC	8 cut(s)	254, 269, 449, 929, 1028, 1052, 1065, 1211
AcoI	YGGCCR	2 cut(s)	12, 414
AcsI	RAATTY	2 cut(s)	141, 707
AcvI	CACGTG	1 cut(s)	283
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	1 cut(s)	656
AgsI	TTSAA	6 cut(s)	66, 147, 258, 358, 502, 1171
AjnI	CCWGG	4 cut(s)	119, 242, 568, 1138
AluBI	AGCT	6 cut(s)	94, 178, 187, 253, 748, 1385
AluI	AGCT	6 cut(s)	94, 178, 187, 253, 748, 1385
Alw21I	GWGCWC	1 cut(s)	189
Alw26I	GTCTC	3 cut(s)	209, 690, 935
AlwI	GGATC	8 cut(s)	254, 269, 449, 929, 1028, 1052, 1065, 1211
AlwNI	CAGNNNCTG	1 cut(s)	1385
Ama87I	CYCGRG	3 cut(s)	107, 938, 1222
AoxI	GGCC	7 cut(s)	12, 84, 414, 664, 985, 1032, 1226
ApeKI	GCWGC	3 cut(s)	238, 748, 1178
ApoI	RAATTY	2 cut(s)	141, 707
ArsI	GACNNNNNNTTYG	2 cut(s)	719, 751
Asp700I	GAANNNNTTC	1 cut(s)	1386
AspLEI	GCGC	4 cut(s)	238, 430, 466, 720
AspS9I	GGNCC	5 cut(s)	85, 111, 123, 986, 1032
AsuC2I	CCSGG	1 cut(s)	83
AsuHPI	GGTGA	1 cut(s)	638
AvaI	CYCGRG	3 cut(s)	107, 938, 1222
AvaII	GGWCC	2 cut(s)	111, 123
AxyI	CCTNAGG	1 cut(s)	1230
BaeGI	GKGCMC	1 cut(s)	1313
BalI	TGGCCA	2 cut(s)	14, 416
BamHI	GGATCC	1 cut(s)	1057
BanI	GGYRCC	1 cut(s)	1135
BanII	GRGCYC	2 cut(s)	189, 308
BbrPI	CACGTG	1 cut(s)	283
Bbv12I	GWGCWC	1 cut(s)	189
BbvI	GCAGC	3 cut(s)	225, 735, 1165
BccI	CCATC	5 cut(s)	111, 425, 656, 808, 917
BceAI	ACGGC	1 cut(s)	184
BciT130I	CCWGG	4 cut(s)	121, 244, 570, 1140
BclI	TGATCA	2 cut(s)	285, 586
BcnI	CCSGG	1 cut(s)	83
BcoDI	GTCTC	3 cut(s)	209, 690, 935
BfaI	CTAG	6 cut(s)	632, 641, 1017, 1314, 1331, 1396
BfmI	CTRYAG	1 cut(s)	1062
BfoI	RGCGCY	1 cut(s)	239
BfuAI	ACCTGC	1 cut(s)	1055
BglI	GCCNNNNNGGC	1 cut(s)	1317
BglII	AGATCT	2 cut(s)	513, 1093
BisI	GCNGC	5 cut(s)	231, 234, 239, 749, 1179
BlsI	GCNGC	5 cut(s)	232, 235, 240, 750, 1180
Bme1390I	CCNGG	5 cut(s)	83, 121, 244, 570, 1140
Bme18I	GGWCC	2 cut(s)	111, 123
BmeT110I	CYCGRG	3 cut(s)	107, 938, 1222
BmgT120I	GGNCC	5 cut(s)	85, 111, 123, 986, 1032
BmiI	GGNNCC	7 cut(s)	112, 125, 137, 1033, 1059, 1137, 1356
BmrFI	CCNGG	5 cut(s)	83, 121, 244, 570, 1140
BmsI	GCATC	2 cut(s)	1093, 1251
BoxI	GACNNNNGTC	1 cut(s)	458
BpmI	CTGGAG	1 cut(s)	1166
BpuEI	CTTGAG	4 cut(s)	74, 830, 938, 1179
BpuMI	CCSGG	1 cut(s)	83
BsaAI	YACGTR	1 cut(s)	283
BsaBI	GATNNNNATC	2 cut(s)	483, 1107
BsaHI	GRCGYC	1 cut(s)	5
BsaJI	CCNNGG	5 cut(s)	9, 119, 242, 313, 568
Bse1I	ACTGG	1 cut(s)	1149
Bse21I	CCTNAGG	1 cut(s)	1230
Bse3DI	GCAATG	1 cut(s)	727
Bse8I	GATNNNNATC	2 cut(s)	483, 1107
BseBI	CCWGG	4 cut(s)	121, 244, 570, 1140
BseDI	CCNNGG	5 cut(s)	9, 119, 242, 313, 568
BseGI	GGATG	5 cut(s)	484, 844, 928, 1075, 1158
BseJI	GATNNNNATC	2 cut(s)	483, 1107
BseMI	GCAATG	1 cut(s)	727
BseMII	CTCAG	3 cut(s)	984, 1074, 1244
BseNI	ACTGG	1 cut(s)	1149
BseSI	GKGCMC	1 cut(s)	1313
BseXI	GCAGC	3 cut(s)	225, 735, 1165
BseYI	CCCAGC	1 cut(s)	1320
BsgI	GTGCAG	2 cut(s)	675, 1019
Bsh1236I	CGCG	3 cut(s)	315, 428, 464
Bsh1285I	CGRYCG	1 cut(s)	408
BshFI	GGCC	7 cut(s)	14, 86, 416, 666, 987, 1034, 1228
BshNI	GGYRCC	1 cut(s)	1135
BsiEI	CGRYCG	1 cut(s)	408
BsiHKAI	GWGCWC	1 cut(s)	189
BsiHKCI	CYCGRG	3 cut(s)	107, 938, 1222
BsiSI	CCGG	3 cut(s)	83, 164, 439
BslFI	GGGAC	1 cut(s)	1361
BsmAI	GTCTC	3 cut(s)	209, 690, 935
BsmBI	CGTCTC	2 cut(s)	209, 935
BsmFI	GGGAC	1 cut(s)	1361
BsnI	GGCC	7 cut(s)	14, 86, 416, 666, 987, 1034, 1228
BsoBI	CYCGRG	3 cut(s)	107, 938, 1222
Bsp1286I	GDGCHC	3 cut(s)	189, 308, 1313
Bsp19I	CCATGG	1 cut(s)	9
BspACI	CCGC	7 cut(s)	171, 230, 233, 313, 315, 547, 1361
BspANI	GGCC	7 cut(s)	14, 86, 416, 666, 987, 1034, 1228
BspCNI	CTCAG	3 cut(s)	985, 1075, 1243
BspFNI	CGCG	3 cut(s)	315, 428, 464
BspHI	TCATGA	1 cut(s)	321
BspLI	GGNNCC	7 cut(s)	112, 125, 137, 1033, 1059, 1137, 1356
BspMAI	CTGCAG	1 cut(s)	1066
BspMI	ACCTGC	1 cut(s)	1055
BspPI	GGATC	8 cut(s)	254, 269, 449, 929, 1028, 1052, 1065, 1211
BspQI	GCTCTTC	1 cut(s)	194
BspT107I	GGYRCC	1 cut(s)	1135
BsrDI	GCAATG	1 cut(s)	727
BsrI	ACTGG	1 cut(s)	1149
BssECI	CCNNGG	5 cut(s)	9, 119, 242, 313, 568
BssNI	GRCGYC	1 cut(s)	5
BssT1I	CCWWGG	1 cut(s)	9
Bst2UI	CCWGG	4 cut(s)	121, 244, 570, 1140
Bst4CI	ACNGT	2 cut(s)	949, 1349
Bst6I	CTCTTC	3 cut(s)	194, 354, 1082
BstACI	GRCGYC	1 cut(s)	5
BstBAI	YACGTR	1 cut(s)	283
BstC8I	GCNNGC	3 cut(s)	547, 985, 1008
BstDEI	CTNAG	4 cut(s)	884, 993, 1083, 1230
BstDSI	CCRYGG	2 cut(s)	9, 313
BstEII	GGTNACC	1 cut(s)	626
BstENI	CCTNNNNNAGG	1 cut(s)	1296
BstF5I	GGATG	5 cut(s)	484, 844, 928, 1075, 1158
BstFNI	CGCG	3 cut(s)	315, 428, 464
BstH2I	RGCGCY	1 cut(s)	239
BstHHI	GCGC	4 cut(s)	238, 430, 466, 720
BstMAI	GTCTC	3 cut(s)	209, 690, 935
BstMCI	CGRYCG	1 cut(s)	408
BstMWI	GCNNNNNNNGC	9 cut(s)	11, 184, 303, 312, 444, 672, 737, 1317, 1391
BstNI	CCWGG	4 cut(s)	121, 244, 570, 1140
BstNSI	RCATGY	2 cut(s)	776, 1010
BstPAI	GACNNNNGTC	1 cut(s)	458
BstPI	GGTNACC	1 cut(s)	626
BstSCI	CCNGG	5 cut(s)	81, 119, 242, 568, 1138
BstSFI	CTRYAG	1 cut(s)	1062
BstSLI	GKGCMC	1 cut(s)	1313
BstUI	CGCG	3 cut(s)	315, 428, 464
BstV1I	GCAGC	3 cut(s)	225, 735, 1165
BstX2I	RGATCY	4 cut(s)	441, 513, 1057, 1093
BstXI	CCANNNNNNTGG	1 cut(s)	888
BstYI	RGATCY	4 cut(s)	441, 513, 1057, 1093
Bsu36I	CCTNAGG	1 cut(s)	1230
BsuRI	GGCC	7 cut(s)	14, 86, 416, 666, 987, 1034, 1228
BtgI	CCRYGG	2 cut(s)	9, 313
BtsCI	GGATG	5 cut(s)	484, 844, 928, 1075, 1158
BtsI	GCAGTG	1 cut(s)	1007
BtsIMutI	CAGTG	1 cut(s)	1007
BveI	ACCTGC	1 cut(s)	1055
Cac8I	GCNNGC	3 cut(s)	547, 985, 1008
CaiI	CAGNNNCTG	1 cut(s)	1385
CciI	TCATGA	1 cut(s)	321
CfoI	GCGC	4 cut(s)	238, 430, 466, 720
Cfr13I	GGNCC	5 cut(s)	85, 111, 123, 986, 1032
Cfr42I	CCGCGG	1 cut(s)	316
CseI	GACGC	2 cut(s)	434, 737
Csp6I	GTAC	1 cut(s)	655
CviAII	CATG	9 cut(s)	10, 197, 322, 773, 905, 1007, 1112, 1132, 1306
CviQI	GTAC	1 cut(s)	655
DdeI	CTNAG	4 cut(s)	884, 993, 1083, 1230
EaeI	YGGCCR	2 cut(s)	12, 414
Eam1104I	CTCTTC	3 cut(s)	194, 354, 1082
EarI	CTCTTC	3 cut(s)	194, 354, 1082
Ecl136II	GAGCTC	1 cut(s)	187
Eco130I	CCWWGG	1 cut(s)	9
Eco147I	AGGCCT	1 cut(s)	1228
Eco24I	GRGCYC	2 cut(s)	189, 308
Eco47I	GGWCC	2 cut(s)	111, 123
Eco53kI	GAGCTC	1 cut(s)	187
Eco72I	CACGTG	1 cut(s)	283
Eco81I	CCTNAGG	1 cut(s)	1230
Eco88I	CYCGRG	3 cut(s)	107, 938, 1222
Eco91I	GGTNACC	1 cut(s)	626
EcoICRI	GAGCTC	1 cut(s)	187
EcoNI	CCTNNNNNAGG	1 cut(s)	1296
EcoO109I	RGGNCCY	1 cut(s)	123
EcoO65I	GGTNACC	1 cut(s)	626
EcoRI	GAATTC	1 cut(s)	707
EcoRII	CCWGG	4 cut(s)	119, 242, 568, 1138
EcoT14I	CCWWGG	1 cut(s)	9
EcoT38I	GRGCYC	2 cut(s)	189, 308
ErhI	CCWWGG	1 cut(s)	9
Esp3I	CGTCTC	2 cut(s)	209, 935
FaeI	CATG	9 cut(s)	13, 200, 325, 776, 908, 1010, 1115, 1135, 1309
FaqI	GGGAC	1 cut(s)	1361
FatI	CATG	9 cut(s)	9, 196, 321, 772, 904, 1006, 1111, 1131, 1305
FauI	CCCGC	2 cut(s)	164, 554
FbaI	TGATCA	2 cut(s)	285, 586
FblI	GTMKAC	1 cut(s)	471
Fnu4HI	GCNGC	5 cut(s)	231, 234, 239, 749, 1179
FokI	GGATG	5 cut(s)	491, 831, 935, 1062, 1145
FriOI	GRGCYC	2 cut(s)	189, 308
Fsp4HI	GCNGC	5 cut(s)	231, 234, 239, 749, 1179
FspBI	CTAG	6 cut(s)	632, 641, 1017, 1314, 1331, 1396
GlaI	GCGC	4 cut(s)	237, 429, 465, 719
GluI	GCNGC	5 cut(s)	231, 234, 239, 749, 1179
GsaI	CCCAGC	1 cut(s)	1324
GsuI	CTGGAG	1 cut(s)	1166
HaeII	RGCGCY	1 cut(s)	239
HaeIII	GGCC	7 cut(s)	14, 86, 416, 666, 987, 1034, 1228
HapII	CCGG	3 cut(s)	83, 164, 439
HgaI	GACGC	2 cut(s)	434, 737
HhaI	GCGC	4 cut(s)	238, 430, 466, 720
Hin1I	GRCGYC	1 cut(s)	5
Hin1II	CATG	9 cut(s)	13, 200, 325, 776, 908, 1010, 1115, 1135, 1309
Hin6I	GCGC	4 cut(s)	236, 428, 464, 718
HinP1I	GCGC	4 cut(s)	236, 428, 464, 718
HincII	GTYRAC	2 cut(s)	472, 1336
HindII	GTYRAC	2 cut(s)	472, 1336
HindIII	AAGCTT	1 cut(s)	251
HinfI	GANTC	8 cut(s)	200, 318, 329, 369, 449, 459, 473, 1302
HpaII	CCGG	3 cut(s)	83, 164, 439
HphI	GGTGA	1 cut(s)	638
Hpy166II	GTNNAC	5 cut(s)	280, 472, 575, 763, 1336
Hpy188I	TCNGA	6 cut(s)	140, 334, 368, 478, 489, 680
Hpy188III	TCNNGA	8 cut(s)	322, 641, 809, 917, 938, 1055, 1232, 1295
Hpy8I	GTNNAC	5 cut(s)	280, 472, 575, 763, 1336
Hpy99I	CGWCG	5 cut(s)	185, 425, 428, 801, 804
HpyAV	CCTTC	6 cut(s)	202, 287, 904, 1172, 1177, 1292
HpyCH4III	ACNGT	2 cut(s)	949, 1349
HpyCH4IV	ACGT	2 cut(s)	282, 876
HpyCH4V	TGCA	8 cut(s)	605, 692, 976, 1000, 1010, 1028, 1064, 1274
HpyF10VI	GCNNNNNNNGC	9 cut(s)	11, 184, 303, 312, 444, 672, 737, 1317, 1391
HpyF3I	CTNAG	4 cut(s)	884, 993, 1083, 1230
HpySE526I	ACGT	2 cut(s)	282, 876
Hsp92I	GRCGYC	1 cut(s)	5
Hsp92II	CATG	9 cut(s)	13, 200, 325, 776, 908, 1010, 1115, 1135, 1309
HspAI	GCGC	4 cut(s)	236, 428, 464, 718
Ksp22I	TGATCA	2 cut(s)	285, 586
KspI	CCGCGG	1 cut(s)	316
LguI	GCTCTTC	1 cut(s)	194
LmnI	GCTCC	3 cut(s)	141, 1147, 1360
Lsp1109I	GCAGC	3 cut(s)	225, 735, 1165
LweI	GCATC	2 cut(s)	1093, 1251
MaeI	CTAG	6 cut(s)	632, 641, 1017, 1314, 1331, 1396
MaeII	ACGT	2 cut(s)	282, 876
MaeIII	GTNAC	1 cut(s)	626
MboII	GAAGA	8 cut(s)	70, 181, 217, 232, 341, 545, 716, 1099
MflI	RGATCY	4 cut(s)	441, 513, 1057, 1093
MhlI	GDGCHC	3 cut(s)	189, 308, 1313
MlsI	TGGCCA	2 cut(s)	14, 416
MluCI	AATT	4 cut(s)	141, 358, 610, 707
MluNI	TGGCCA	2 cut(s)	14, 416
MlyI	GAGTC	4 cut(s)	209, 363, 467, 468
MmeI	TCCRAC	2 cut(s)	391, 1103
Mox20I	TGGCCA	2 cut(s)	14, 416
MroXI	GAANNNNTTC	1 cut(s)	1386
MscI	TGGCCA	2 cut(s)	14, 416
MseI	TTAA	2 cut(s)	1127, 1155
MslI	CAYNNNNRTG	1 cut(s)	1259
Msp20I	TGGCCA	2 cut(s)	14, 416
MspA1I	CMGCKG	2 cut(s)	315, 748
MspI	CCGG	3 cut(s)	83, 164, 439
MspR9I	CCNGG	5 cut(s)	83, 121, 244, 570, 1140
MvaI	CCWGG	4 cut(s)	121, 244, 570, 1140
MvnI	CGCG	3 cut(s)	315, 428, 464
MwoI	GCNNNNNNNGC	9 cut(s)	11, 184, 303, 312, 444, 672, 737, 1317, 1391
NciI	CCSGG	1 cut(s)	83
NcoI	CCATGG	1 cut(s)	9
NlaIII	CATG	9 cut(s)	13, 200, 325, 776, 908, 1010, 1115, 1135, 1309
NlaIV	GGNNCC	7 cut(s)	112, 125, 137, 1033, 1059, 1137, 1356
NmuCI	GTSAC	1 cut(s)	626
NspI	RCATGY	2 cut(s)	776, 1010
PaeI	GCATGC	1 cut(s)	1010
PaeR7I	CTCGAG	1 cut(s)	1222
PagI	TCATGA	1 cut(s)	321
PceI	AGGCCT	1 cut(s)	1228
PciSI	GCTCTTC	1 cut(s)	194
PcsI	WCGNNNNNNNCGW	4 cut(s)	156, 189, 288, 468
PdmI	GAANNNNTTC	1 cut(s)	1386
PfeI	GAWTC	4 cut(s)	318, 329, 449, 1302
PkrI	GCNGC	5 cut(s)	232, 235, 240, 750, 1180
Ple19I	CGATCG	1 cut(s)	408
PleI	GAGTC	4 cut(s)	208, 363, 467, 467
PmaCI	CACGTG	1 cut(s)	283
PmlI	CACGTG	1 cut(s)	283
PpsI	GAGTC	4 cut(s)	208, 363, 467, 467
Ppu21I	YACGTR	1 cut(s)	283
PpuMI	RGGWCCY	1 cut(s)	123
PshAI	GACNNNNGTC	1 cut(s)	458
Psp124BI	GAGCTC	1 cut(s)	189
Psp5II	RGGWCCY	1 cut(s)	123
Psp6I	CCWGG	4 cut(s)	119, 242, 568, 1138
PspCI	CACGTG	1 cut(s)	283
PspEI	GGTNACC	1 cut(s)	626
PspFI	CCCAGC	1 cut(s)	1320
PspGI	CCWGG	4 cut(s)	119, 242, 568, 1138
PspN4I	GGNNCC	7 cut(s)	112, 125, 137, 1033, 1059, 1137, 1356
PspPI	GGNCC	5 cut(s)	85, 111, 123, 986, 1032
PspPPI	RGGWCCY	1 cut(s)	123
PspXI	VCTCGAGB	1 cut(s)	1222
PstI	CTGCAG	1 cut(s)	1066
PstNI	CAGNNNCTG	1 cut(s)	1385
PsuI	RGATCY	4 cut(s)	441, 513, 1057, 1093
PvuI	CGATCG	1 cut(s)	408
PvuII	CAGCTG	1 cut(s)	748
RsaI	GTAC	1 cut(s)	656
RsaNI	GTAC	1 cut(s)	655
RseI	CAYNNNNRTG	1 cut(s)	1259
SacI	GAGCTC	1 cut(s)	189
SacII	CCGCGG	1 cut(s)	316
SalI	GTCGAC	1 cut(s)	470
SapI	GCTCTTC	1 cut(s)	194
SaqAI	TTAA	2 cut(s)	1127, 1155
SatI	GCNGC	5 cut(s)	231, 234, 239, 749, 1179
Sau96I	GGNCC	5 cut(s)	85, 111, 123, 986, 1032
SbfI	CCTGCAGG	1 cut(s)	1066
SchI	GAGTC	4 cut(s)	209, 363, 467, 468
ScrFI	CCNGG	5 cut(s)	83, 121, 244, 570, 1140
SdaI	CCTGCAGG	1 cut(s)	1066
SduI	GDGCHC	3 cut(s)	189, 308, 1313
SfaNI	GCATC	2 cut(s)	1093, 1251
SfcI	CTRYAG	1 cut(s)	1062
Sfr274I	CTCGAG	1 cut(s)	1222
Sfr303I	CCGCGG	1 cut(s)	316
SgrBI	CCGCGG	1 cut(s)	316
SinI	GGWCC	2 cut(s)	111, 123
SlaI	CTCGAG	1 cut(s)	1222
SmiMI	CAYNNNNRTG	1 cut(s)	1259
SmlI	CTYRAG	5 cut(s)	89, 809, 917, 1194, 1222
SmoI	CTYRAG	5 cut(s)	89, 809, 917, 1194, 1222
SphI	GCATGC	1 cut(s)	1010
Sse8387I	CCTGCAGG	1 cut(s)	1066
Sse9I	AATT	4 cut(s)	141, 358, 610, 707
SseBI	AGGCCT	1 cut(s)	1228
SsiI	CCGC	7 cut(s)	171, 230, 233, 313, 315, 547, 1361
SspMI	CTAG	6 cut(s)	632, 641, 1017, 1314, 1331, 1396
SstI	GAGCTC	1 cut(s)	189
StuI	AGGCCT	1 cut(s)	1228
StyD4I	CCNGG	5 cut(s)	81, 119, 242, 568, 1138
StyI	CCWWGG	1 cut(s)	9
TaaI	ACNGT	2 cut(s)	949, 1349
TaiI	ACGT	2 cut(s)	285, 879
TasI	AATT	4 cut(s)	141, 358, 610, 707
TatI	WGTACW	1 cut(s)	654
TauI	GCSGC	2 cut(s)	233, 236
TfiI	GAWTC	4 cut(s)	318, 329, 449, 1302
Tru1I	TTAA	2 cut(s)	1127, 1155
Tru9I	TTAA	2 cut(s)	1127, 1155
TscAI	CASTG	1 cut(s)	1007
TseFI	GTSAC	1 cut(s)	626
TseI	GCWGC	3 cut(s)	238, 748, 1178
Tsp45I	GTSAC	1 cut(s)	626
TspDTI	ATGAA	6 cut(s)	310, 341, 833, 911, 1234, 1294
TspGWI	ACGGA	3 cut(s)	388, 553, 689
TspRI	CASTG	1 cut(s)	1007
VpaK11BI	GGWCC	2 cut(s)	111, 123
XagI	CCTNNNNNAGG	1 cut(s)	1296
XapI	RAATTY	2 cut(s)	141, 707
XbaI	TCTAGA	1 cut(s)	640
XceI	RCATGY	2 cut(s)	776, 1010
XhoI	CTCGAG	1 cut(s)	1222
XmiI	GTMKAC	1 cut(s)	471
XmnI	GAANNNNTTC	1 cut(s)	1386
XspI	CTAG	6 cut(s)	632, 641, 1017, 1314, 1331, 1396

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.