Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0011324

Physical Location & Seq

Genomic Coordinates Reverse (-)

20606255 .. 20610256

Gene Structure 4002 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1216300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2751 bp

ATGGCTCCGACGCCGTCTTCTTCCAAATTAAACCACTGCCACACAACCCAGTTCAAGACTCCGCTGTCCAAGCACCGCCTCCAGTTTCCGAAAGCCCACCCATCTCCGACCCCTAACTCAGCAGTAAAAGACCCACCGGCGGAGCACCCGGTCGAAGTCCTAGCCCGAATCCGAGACCACCCAGATCGAAAAGAACAACCCGCATCGGTTCTCCAGATCAATCCTCAGAGGCAGAGTATTCGGGTCCGGGCCGATTTTGGATATCGGGATTTCACGCTCGATGGGGTTTCTTTGTCGGAAGAAGACGACTTGGGGACGTTTTACAGAAAATTTGTTCAGTCCAGGATTGACAGTGTGAAATTGGGGGAAAAATGTACGATCATGATGTACGGACCCACTGGTTCAGGGAAGAGCCACACCATGTTCGGGTGTTGCAAGCAGCCTGGGATTGTTTACAGGTCGCTGAAAGATATTCTTGGGGACGGAGAAGACGATGGTGGCACGGCGGTTGGGTCGTTTGTGCAGGTCACTGTTTTGGAGATTTACAATGAGGAGATTTATGATTTGTTGTCGAGCAATGGCGGGGGAGGATTCGGGTTTGGATGGCCAAAGAGCAATGGCTCCAATTCTAAGGTTAGGCTTGAAGTGATGGGAAAGAAGGCAAAGAATGCAACCTACATTTCTGGAAATGAAGCTGGGAAAATTTCAAAAGAGATTCAGAAAGTGGAAAAGAGAAGGATTGTTAGGAGCACCCTTTGCAATGATAGAAGTTCTCGAAGCCATTGCATGATAATCCTTGATGTGCCAACAGTGGGAGGCCGCTTGATGCTTGTGGACATGGCTGGATCTGAAAATATCGAGCAAGCAGGCCAAATTGGGTTCGAGGCAAAAATGCAGACTGCAAAGATCAACCAAGGAAACATAGCTTTGAAAAGGGTGGTTGAATCCATTGCCAATGGCGATTCTCATGTGCCCTTCAGGGACAGCAAACTGACCATGCTTCTACAGGATTCTTTTGAGGACGACAAGACAAAAATTTTAATGGTCCTGTGTGCAAGTCCAGACCCAAAGGAGATACACAAGACAATCTCCACCCTTGAATATGGTGCAAAAGCAAAATGTATTGTCCGCGGCCCTCATACACCAATCAAGGACAAAACTGGGACTGAAGATGCATCTGCAGTTATTTTAGGATCAAGGATTGCTGCCATGGATGAATTTATCCTTAAACTGCAAAGGGAGAATAAGCTCAAAGAGAAAGAGCGGAATGAAGCACACAAGGAGCTAATGAAGAAAGAAGAGGAGGTTGCTGCTCTGAGATCTAAACTTGAGCTTACAGAAGGGAAGAGATCTGGGGCAAAGGAGGAAGAAATCAACTTGAAGGTGAATGAAGTGGCCCAGACTCTGAAATATGAGTTGGAAAAGAAATTGGCTGAATGCCAGAGAATGGCTAATGAGTTCGTTGAACTAGAGAGAAGGAGAATGGAAGAAAGGATTTTGCAACAGCAGCAGGAAGTTGAGATGCTGAGAAAGCGGTTGGAGGAAATTGAGCTTGAACTGTGCCGCTCAAGTGATGGAAATGGCAAGGAGAGCGGGTCAAAGGAGCCCGATGGAACGCAGTTTGCCAAAAGGTTATTAGGAATCTATGCTAGTGATGATGCAGGAGGAATGGTAAAGTCAATGGATTTAGACATGGATGATCAAGAACCGTTTGCTCGGGAGATCAAATATGTAGGAGTGGTACCTGGGGTTGCTTCTCAATCTGACAGCAGTATTGTAAATGCTGCACACATTGATTCTTTTGAACCAAAATATGCTGACAGGGTATGCCTAAGCACTGTTTTTGAGGAAGAAGAAATGGAAGAAGAGGAAGGAGCTAATGAAAATGTGGAAGCTGAAGAGGTTGAGAAAGAAGTTATAGAGGAAAAGAGAGTGTGTGTGGTTGATGGATCTAGCTTCAGGAGTAATTATAATGTGGGCTACCTGACATCTTCACCTCAGGAATATCAAGATACACCAACTGTGCATTGGCAAAGCAGAAATCCAGAGGAAGAACATAGCAATGGAAAACCAGATGATAGGCTTGTCAGAATCCGCAACATATTTACACTCTGTGGAAACTACAGAGAGCTATCTCAGCATATCCCAAGTCAAGCACCAACTGTTGCATCAGTTAAATCAGAAAATGAGCAAAAATTGGTGGAACCAGAGATAAACAAAAGTCGAGTGTGTGATGAGAGAGCATTGGCTTCCAAAGAAAACTGTGACCCTTCAAATGAGGGCACTGATTCGGATACAACTGAAGTGTATGTGAAATGGGAGGGCTCAAAAGAAAATCCAGGGAAGTTTATTACAACACTGAAGGTTGTAAAAGACGCAACCCTTGCTGAACTGCGAAAGTTGATTGAAATCTATCTCGGGGCAGACAATCAAGCATTCACTTTTCTCATGCTTGGGGACCCGAGTGGAGCTTCAGTAGCGAAGGAGAAGGAAGCAGCAACTCAGGCAACAAACCTGCCCCTATGCGACAATCAATCAAATGGCTACCTGGCTTGCCTGAGACCACTGAAGGGAATGCAAAGCCCAAGTCAGCTTCTCCCATTAAGTCCTCTTCCATTGAATTCACTGGAAAACCAACTACCACTCACTCCAACTTCCTGCTTCTCAGTCCAAGGTGGTGATTGTACGACACCCAAAGTAGCACCGAACTTAGGTTCTACTCCTTATATCACTGTCAGAAAACATAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005856 GO:0005871 GO:0005875 GO:0006810 GO:0006928 GO:0007017 GO:0007018 GO:0008017 GO:0008092 GO:0008150 GO:0008574 GO:0009524 GO:0009987 GO:0010970 GO:0015630 GO:0015631 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0030705 GO:0032991 GO:0042623 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0046907 GO:0047496 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051648 GO:0051649 GO:0051650 GO:0051656 GO:0072384 GO:0099111 GO:0099518 GO:1990939

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin

Protein Analysis

917

Amino Acids

101.75

Weight (kDa)

5.58

Isoelectric Point (pI)

52.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Kinesin	PF00225	76 - 375	6.2e-61	Kinesin motor domain
Microtub_bd	PF16796	86 - 190	1.7e-08	Microtubule binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011324)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G14330
fragaria_vesca	FvH4_4g04000 FvH4_4g04000
malus_domestica	MD13G1216300.v1.1 MD16G1221700.v1.1
prunus_persica	Prupe.1G042100_v2.0.a1 Prupe.4G184200_v2.0.a1
pyrus_communis	pycom13g18980 pycom16g18590
rosa_chinensis	RchiOBHm_Chr4g0394251
rosa_laevigata	RLG00000009711
rosa_multiflora	Rmu_sc0007246.1_g000005 Rmu_ssc0000473.1_g000031
rosa_roxburghii	Rroxscaffold_5G00339300
rosa_rugosa	Rorug03G0368300.1
rosa_samantha	Rh4AG051100 Rh4BG048100 Rh4CG054800 Rh4DG047800
rosa_wichuraiana	Rw4G004070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1971
AasI	GACNNNNNNGTC	1 cut(s)	64
Acc36I	ACCTGC	2 cut(s)	514, 2523
Acc65I	GGTACC	1 cut(s)	1741
AccB1I	GGYRCC	1 cut(s)	1741
AccB7I	CCANNNNNTGG	1 cut(s)	1447
AccBSI	CCGCTC	3 cut(s)	1264, 1566, 1593
AccII	CGCG	1 cut(s)	1131
AclWI	GGATC	3 cut(s)	853, 1201, 1957
AcoI	YGGCCR	1 cut(s)	605
AcsI	RAATTY	5 cut(s)	329, 702, 1035, 1217, 2620
AcuI	CTGAAG	8 cut(s)	961, 1188, 1917, 1942, 2322, 2381, 2457, 2588
AcyI	GRCGYC	1 cut(s)	11
AdeI	CACNNNGTG	1 cut(s)	2114
AfaI	GTAC	4 cut(s)	376, 389, 1743, 2686
AfiI	CCNNNNNNNGG	6 cut(s)	139, 426, 812, 1447, 2570, 2711
AjnI	CCWGG	5 cut(s)	341, 442, 1744, 2338, 2547
AjuI	GAANNNNNNNTTGG	2 cut(s)	1400, 1432
Alw21I	GWGCWC	2 cut(s)	147, 752
Alw26I	GTCTC	2 cut(s)	168, 2554
AlwI	GGATC	3 cut(s)	853, 1201, 1957
AlwNI	CAGNNNCTG	1 cut(s)	1405
Ama87I	CYCGRG	3 cut(s)	1716, 2417, 2461
AoxI	GGCC	6 cut(s)	249, 605, 817, 868, 1132, 1395
ApeKI	GCWGC	6 cut(s)	439, 1205, 1310, 1507, 1784, 2495
ApoI	RAATTY	5 cut(s)	329, 702, 1035, 1217, 2620
ArsI	GACNNNNNNTTYG	2 cut(s)	2572, 2604
Asp718I	GGTACC	1 cut(s)	1741
AspS9I	GGNCC	7 cut(s)	244, 249, 392, 1045, 1133, 1396, 2458
AsuC2I	CCSGG	2 cut(s)	149, 248
AsuHPI	GGTGA	3 cut(s)	1396, 1986, 2690
AvaI	CYCGRG	3 cut(s)	1716, 2417, 2461
AvaII	GGWCC	4 cut(s)	244, 392, 1045, 2458
AxyI	CCTNAGG	1 cut(s)	1998
BaeGI	GKGCMC	2 cut(s)	975, 2285
BalI	TGGCCA	1 cut(s)	607
BanI	GGYRCC	1 cut(s)	1741
BanII	GRGCYC	2 cut(s)	1608, 2327
BbsI	GAAGAC	3 cut(s)	9, 309, 495
Bbv12I	GWGCWC	2 cut(s)	147, 752
BbvI	GCAGC	6 cut(s)	451, 1192, 1297, 1519, 1771, 2507
BccI	CCATC	8 cut(s)	109, 275, 488, 597, 643, 1568, 1604, 1940
BceAI	ACGGC	1 cut(s)	519
BcgI	CGANNNNNNTGC	4 cut(s)	221, 255, 765, 799
BciT130I	CCWGG	5 cut(s)	343, 444, 1746, 2340, 2549
BciVI	GTATCC	1 cut(s)	2287
BclI	TGATCA	1 cut(s)	1699
BcnI	CCSGG	2 cut(s)	149, 248
BcoDI	GTCTC	2 cut(s)	168, 2554
BfaI	CTAG	4 cut(s)	161, 1469, 1650, 1953
BfmI	CTRYAG	3 cut(s)	1004, 1179, 2122
BfuAI	ACCTGC	2 cut(s)	514, 2523
BfuI	GTATCC	1 cut(s)	2287
BglII	AGATCT	2 cut(s)	1319, 1349
BisI	GCNGC	9 cut(s)	440, 820, 1132, 1206, 1311, 1508, 1564, 1785, 2496
BlsI	GCNGC	9 cut(s)	441, 821, 1133, 1207, 1312, 1509, 1565, 1786, 2497
Bme1390I	CCNGG	7 cut(s)	149, 248, 343, 444, 1746, 2340, 2549
Bme18I	GGWCC	4 cut(s)	244, 392, 1045, 2458
BmeT110I	CYCGRG	3 cut(s)	1716, 2417, 2461
BmgT120I	GGNCC	7 cut(s)	244, 249, 392, 1045, 1133, 1396, 2458
BmiI	GGNNCC	9 cut(s)	6, 245, 394, 622, 1605, 1743, 2205, 2459, 2460
BmrFI	CCNGG	7 cut(s)	149, 248, 343, 444, 1746, 2340, 2549
BmrI	ACTGGG	2 cut(s)	43, 1170
BmsI	GCATC	7 cut(s)	212, 816, 1162, 1184, 1512, 1648, 2177
BmuI	ACTGGG	2 cut(s)	43, 1170
BpiI	GAAGAC	3 cut(s)	9, 309, 495
BpmI	CTGGAG	2 cut(s)	65, 197
Bpu10I	CCTNAGC	1 cut(s)	1832
BpuEI	CTTGAG	2 cut(s)	1349, 1552
BpuMI	CCSGG	2 cut(s)	149, 248
BsaBI	GATNNNNATC	1 cut(s)	2408
BsaHI	GRCGYC	1 cut(s)	11
BsaI	GGTCTC	2 cut(s)	168, 2554
BsaJI	CCNNGG	7 cut(s)	443, 913, 1129, 1209, 1745, 2339, 2671
BsaXI	ACNNNNNCTCC	6 cut(s)	1580, 1610, 1656, 1686, 2460, 2490
Bsc4I	CCNNNNNNNGG	6 cut(s)	139, 426, 812, 1447, 2570, 2711
Bse118I	RCCGGY	1 cut(s)	136
Bse1I	ACTGG	5 cut(s)	49, 82, 403, 1165, 2631
Bse21I	CCTNAGG	1 cut(s)	1998
Bse3DI	GCAATG	6 cut(s)	583, 622, 766, 781, 948, 2068
Bse8I	GATNNNNATC	1 cut(s)	2408
BseBI	CCWGG	5 cut(s)	343, 444, 1746, 2340, 2549
BseDI	CCNNGG	7 cut(s)	443, 913, 1129, 1209, 1745, 2339, 2671
BseGI	GGATG	3 cut(s)	608, 1219, 1702
BseJI	GATNNNNATC	1 cut(s)	2408
BseLI	CCNNNNNNNGG	6 cut(s)	139, 426, 812, 1447, 2570, 2711
BseMI	GCAATG	6 cut(s)	583, 622, 766, 781, 948, 2068
BseMII	CTCAG	9 cut(s)	132, 239, 1307, 1517, 2012, 2150, 2516, 2549, 2679
BseNI	ACTGG	5 cut(s)	49, 82, 403, 1165, 2631
BseRI	GAGGAG	2 cut(s)	566, 1316
BseSI	GKGCMC	2 cut(s)	975, 2285
BseXI	GCAGC	6 cut(s)	451, 1192, 1297, 1519, 1771, 2507
BseYI	CCCAGC	1 cut(s)	695
BsgI	GTGCAG	2 cut(s)	542, 1770
Bsh1236I	CGCG	1 cut(s)	1131
Bsh1285I	CGRYCG	1 cut(s)	153
BshFI	GGCC	6 cut(s)	251, 607, 819, 870, 1134, 1397
BshNI	GGYRCC	1 cut(s)	1741
BsiEI	CGRYCG	1 cut(s)	153
BsiHKAI	GWGCWC	2 cut(s)	147, 752
BsiHKCI	CYCGRG	3 cut(s)	1716, 2417, 2461
BsiSI	CCGG	3 cut(s)	137, 149, 247
BslFI	GGGAC	5 cut(s)	328, 494, 995, 1177, 2471
BslI	CCNNNNNNNGG	6 cut(s)	139, 426, 812, 1447, 2570, 2711
BsmAI	GTCTC	2 cut(s)	168, 2554
BsmFI	GGGAC	5 cut(s)	328, 494, 995, 1177, 2471
BsmI	GAATGC	4 cut(s)	673, 1442, 2435, 2580
BsnI	GGCC	6 cut(s)	251, 607, 819, 870, 1134, 1397
Bso31I	GGTCTC	2 cut(s)	168, 2554
BsoBI	CYCGRG	3 cut(s)	1716, 2417, 2461
Bsp1286I	GDGCHC	6 cut(s)	147, 752, 975, 1608, 2285, 2327
Bsp19I	CCATGG	1 cut(s)	1209
BspANI	GGCC	6 cut(s)	251, 607, 819, 870, 1134, 1397
BspCNI	CTCAG	9 cut(s)	131, 238, 1308, 1518, 2011, 2149, 2515, 2550, 2678
BspFNI	CGCG	1 cut(s)	1131
BspHI	TCATGA	1 cut(s)	381
BspLI	GGNNCC	9 cut(s)	6, 245, 394, 622, 1605, 1743, 2205, 2459, 2460
BspMAI	CTGCAG	1 cut(s)	1183
BspMI	ACCTGC	2 cut(s)	514, 2523
BspPI	GGATC	3 cut(s)	853, 1201, 1957
BspQI	GCTCTTC	1 cut(s)	404
BspT107I	GGYRCC	1 cut(s)	1741
BspTNI	GGTCTC	2 cut(s)	168, 2554
BsrBI	CCGCTC	3 cut(s)	1264, 1566, 1593
BsrDI	GCAATG	6 cut(s)	583, 622, 766, 781, 948, 2068
BsrFI	RCCGGY	1 cut(s)	136
BsrI	ACTGG	5 cut(s)	49, 82, 403, 1165, 2631
BssAI	RCCGGY	1 cut(s)	136
BssECI	CCNNGG	7 cut(s)	443, 913, 1129, 1209, 1745, 2339, 2671
BssNI	GRCGYC	1 cut(s)	11
BssT1I	CCWWGG	3 cut(s)	913, 1209, 2671
Bst2UI	CCWGG	5 cut(s)	343, 444, 1746, 2340, 2549
Bst6I	CTCTTC	6 cut(s)	404, 1293, 1340, 1860, 1893, 2616
BstACI	GRCGYC	1 cut(s)	11
BstAPI	GCANNNNNTGC	3 cut(s)	668, 756, 2384
BstC8I	GCNNGC	4 cut(s)	437, 864, 868, 2554
BstDSI	CCRYGG	2 cut(s)	1129, 1209
BstF5I	GGATG	3 cut(s)	608, 1219, 1702
BstFNI	CGCG	1 cut(s)	1131
BstMAI	GTCTC	2 cut(s)	168, 2554
BstMCI	CGRYCG	1 cut(s)	153
BstNI	CCWGG	5 cut(s)	343, 444, 1746, 2340, 2549
BstSCI	CCNGG	7 cut(s)	147, 246, 341, 442, 1744, 2338, 2547
BstSFI	CTRYAG	3 cut(s)	1004, 1179, 2122
BstSLI	GKGCMC	2 cut(s)	975, 2285
BstUI	CGCG	1 cut(s)	1131
BstV1I	GCAGC	6 cut(s)	451, 1192, 1297, 1519, 1771, 2507
BstV2I	GAAGAC	3 cut(s)	9, 309, 495
BstX2I	RGATCY	4 cut(s)	845, 1319, 1349, 1949
BstYI	RGATCY	4 cut(s)	845, 1319, 1349, 1949
Bsu36I	CCTNAGG	1 cut(s)	1998
BsuI	GTATCC	1 cut(s)	2287
BsuRI	GGCC	6 cut(s)	251, 607, 819, 870, 1134, 1397
BtgI	CCRYGG	2 cut(s)	1129, 1209
BtsCI	GGATG	3 cut(s)	608, 1219, 1702
BtsI	GCAGTG	1 cut(s)	34
BveI	ACCTGC	2 cut(s)	514, 2523
Cac8I	GCNNGC	4 cut(s)	437, 864, 868, 2554
CaiI	CAGNNNCTG	1 cut(s)	1405
CciI	TCATGA	1 cut(s)	381
Cfr10I	RCCGGY	1 cut(s)	136
Cfr13I	GGNCC	7 cut(s)	244, 249, 392, 1045, 1133, 1396, 2458
Cfr42I	CCGCGG	1 cut(s)	1132
CseI	GACGC	2 cut(s)	19, 2384
Csp6I	GTAC	4 cut(s)	375, 388, 1742, 2685
CviAII	CATG	9 cut(s)	382, 421, 787, 838, 968, 997, 1210, 1693, 2449
CviQI	GTAC	4 cut(s)	375, 388, 1742, 2685
DraIII	CACNNNGTG	1 cut(s)	2114
DrdI	GACNNNNNNGTC	1 cut(s)	64
DseDI	GACNNNNNNGTC	1 cut(s)	64
EaeI	YGGCCR	1 cut(s)	605
Eam1104I	CTCTTC	6 cut(s)	404, 1293, 1340, 1860, 1893, 2616
EarI	CTCTTC	6 cut(s)	404, 1293, 1340, 1860, 1893, 2616
EciI	GGCGGA	1 cut(s)	155
Eco130I	CCWWGG	3 cut(s)	913, 1209, 2671
Eco24I	GRGCYC	2 cut(s)	1608, 2327
Eco31I	GGTCTC	2 cut(s)	168, 2554
Eco32I	GATATC	1 cut(s)	263
Eco47I	GGWCC	4 cut(s)	244, 392, 1045, 2458
Eco57I	CTGAAG	8 cut(s)	961, 1188, 1917, 1942, 2322, 2381, 2457, 2588
Eco81I	CCTNAGG	1 cut(s)	1998
Eco88I	CYCGRG	3 cut(s)	1716, 2417, 2461
EcoO109I	RGGNCCY	1 cut(s)	2458
EcoRI	GAATTC	1 cut(s)	2620
EcoRII	CCWGG	5 cut(s)	341, 442, 1744, 2338, 2547
EcoRV	GATATC	1 cut(s)	263
EcoT14I	CCWWGG	3 cut(s)	913, 1209, 2671
EcoT22I	ATGCAT	1 cut(s)	1177
EcoT38I	GRGCYC	2 cut(s)	1608, 2327
ErhI	CCWWGG	3 cut(s)	913, 1209, 2671
FaeI	CATG	9 cut(s)	385, 424, 790, 841, 971, 1000, 1213, 1696, 2452
FalI	AAGNNNNNCTT	2 cut(s)	459, 491
FaqI	GGGAC	5 cut(s)	328, 494, 995, 1177, 2471
FatI	CATG	9 cut(s)	381, 420, 786, 837, 967, 996, 1209, 1692, 2448
FauI	CCCGC	3 cut(s)	208, 575, 1586
FbaI	TGATCA	1 cut(s)	1699
Fnu4HI	GCNGC	9 cut(s)	440, 820, 1132, 1206, 1311, 1508, 1564, 1785, 2496
FokI	GGATG	3 cut(s)	615, 1226, 1709
FriOI	GRGCYC	2 cut(s)	1608, 2327
Fsp4HI	GCNGC	9 cut(s)	440, 820, 1132, 1206, 1311, 1508, 1564, 1785, 2496
FspBI	CTAG	4 cut(s)	161, 1469, 1650, 1953
GluI	GCNGC	9 cut(s)	440, 820, 1132, 1206, 1311, 1508, 1564, 1785, 2496
GsaI	CCCAGC	1 cut(s)	699
GsuI	CTGGAG	2 cut(s)	65, 197
HaeIII	GGCC	6 cut(s)	251, 607, 819, 870, 1134, 1397
HapII	CCGG	3 cut(s)	137, 149, 247
HgaI	GACGC	2 cut(s)	19, 2384
Hin1I	GRCGYC	1 cut(s)	11
Hin1II	CATG	9 cut(s)	385, 424, 790, 841, 971, 1000, 1213, 1696, 2452
HpaII	CCGG	3 cut(s)	137, 149, 247
HphI	GGTGA	3 cut(s)	1396, 1986, 2690
Hpy166II	GTNNAC	2 cut(s)	454, 835
Hpy8I	GTNNAC	2 cut(s)	454, 835
Hpy99I	CGWCG	1 cut(s)	13
HpyCH4IV	ACGT	1 cut(s)	317
HpySE526I	ACGT	1 cut(s)	317
Hsp92I	GRCGYC	1 cut(s)	11
Hsp92II	CATG	9 cut(s)	385, 424, 790, 841, 971, 1000, 1213, 1696, 2452
KflI	GGGWCCC	1 cut(s)	2458
KpnI	GGTACC	1 cut(s)	1745
Ksp22I	TGATCA	1 cut(s)	1699
KspI	CCGCGG	1 cut(s)	1132
LguI	GCTCTTC	1 cut(s)	404
LmnI	GCTCC	8 cut(s)	10, 142, 626, 747, 1282, 1603, 1874, 2468
Lsp1109I	GCAGC	6 cut(s)	451, 1192, 1297, 1519, 1771, 2507
LweI	GCATC	7 cut(s)	212, 816, 1162, 1184, 1512, 1648, 2177
MaeI	CTAG	4 cut(s)	161, 1469, 1650, 1953
MaeII	ACGT	1 cut(s)	317
MaeIII	GTNAC	2 cut(s)	526, 2264
MbiI	CCGCTC	3 cut(s)	1264, 1566, 1593
MflI	RGATCY	4 cut(s)	845, 1319, 1349, 1949
MhlI	GDGCHC	6 cut(s)	147, 752, 975, 1608, 2285, 2327
MlsI	TGGCCA	1 cut(s)	607
MluNI	TGGCCA	1 cut(s)	607
MlyI	GAGTC	2 cut(s)	52, 1396
MmeI	TCCRAC	6 cut(s)	32, 131, 276, 1398, 1518, 2675
Mox20I	TGGCCA	1 cut(s)	607
Mph1103I	ATGCAT	1 cut(s)	1177
MscI	TGGCCA	1 cut(s)	607
MseI	TTAA	5 cut(s)	29, 1040, 1227, 2175, 2603
MslI	CAYNNNNRTG	1 cut(s)	2061
Msp20I	TGGCCA	1 cut(s)	607
MspA1I	CMGCKG	2 cut(s)	64, 1131
MspI	CCGG	3 cut(s)	137, 149, 247
MspR9I	CCNGG	7 cut(s)	149, 248, 343, 444, 1746, 2340, 2549
Mva1269I	GAATGC	4 cut(s)	673, 1442, 2435, 2580
MvaI	CCWGG	5 cut(s)	343, 444, 1746, 2340, 2549
MvnI	CGCG	1 cut(s)	1131
NciI	CCSGG	2 cut(s)	149, 248
NcoI	CCATGG	1 cut(s)	1209
NlaIII	CATG	9 cut(s)	385, 424, 790, 841, 971, 1000, 1213, 1696, 2452
NlaIV	GGNNCC	9 cut(s)	6, 245, 394, 622, 1605, 1743, 2205, 2459, 2460
NmuCI	GTSAC	2 cut(s)	526, 2264
NsiI	ATGCAT	1 cut(s)	1177
PagI	TCATGA	1 cut(s)	381
PciSI	GCTCTTC	1 cut(s)	404
PcsI	WCGNNNNNNNCGW	1 cut(s)	489
PctI	GAATGC	4 cut(s)	673, 1442, 2435, 2580
PflFI	GACNNNGTC	1 cut(s)	13
PflMI	CCANNNNNTGG	1 cut(s)	1447
PfoI	TCCNGGA	1 cut(s)	341
PkrI	GCNGC	9 cut(s)	441, 821, 1133, 1207, 1312, 1509, 1565, 1786, 2497
PleI	GAGTC	2 cut(s)	52, 1396
PpsI	GAGTC	2 cut(s)	52, 1396
PpuMI	RGGWCCY	1 cut(s)	2458
PsiI	TTATAA	1 cut(s)	1971
Psp5II	RGGWCCY	1 cut(s)	2458
Psp6I	CCWGG	5 cut(s)	341, 442, 1744, 2338, 2547
PspFI	CCCAGC	1 cut(s)	695
PspGI	CCWGG	5 cut(s)	341, 442, 1744, 2338, 2547
PspN4I	GGNNCC	9 cut(s)	6, 245, 394, 622, 1605, 1743, 2205, 2459, 2460
PspPI	GGNCC	7 cut(s)	244, 249, 392, 1045, 1133, 1396, 2458
PspPPI	RGGWCCY	1 cut(s)	2458
PstI	CTGCAG	1 cut(s)	1183
PstNI	CAGNNNCTG	1 cut(s)	1405
PsuI	RGATCY	4 cut(s)	845, 1319, 1349, 1949
PsyI	GACNNNGTC	1 cut(s)	13
RsaI	GTAC	4 cut(s)	376, 389, 1743, 2686
RsaNI	GTAC	4 cut(s)	375, 388, 1742, 2685
RseI	CAYNNNNRTG	1 cut(s)	2061
SacII	CCGCGG	1 cut(s)	1132
SapI	GCTCTTC	1 cut(s)	404
SaqAI	TTAA	5 cut(s)	29, 1040, 1227, 2175, 2603
SatI	GCNGC	9 cut(s)	440, 820, 1132, 1206, 1311, 1508, 1564, 1785, 2496
Sau96I	GGNCC	7 cut(s)	244, 249, 392, 1045, 1133, 1396, 2458
SchI	GAGTC	2 cut(s)	52, 1396
ScrFI	CCNGG	7 cut(s)	149, 248, 343, 444, 1746, 2340, 2549
SduI	GDGCHC	6 cut(s)	147, 752, 975, 1608, 2285, 2327
SfaNI	GCATC	7 cut(s)	212, 816, 1162, 1184, 1512, 1648, 2177
SfcI	CTRYAG	3 cut(s)	1004, 1179, 2122
Sfr303I	CCGCGG	1 cut(s)	1132
SgrAI	CRCCGGYG	1 cut(s)	136
SgrBI	CCGCGG	1 cut(s)	1132
SinI	GGWCC	4 cut(s)	244, 392, 1045, 2458
SmiMI	CAYNNNNRTG	1 cut(s)	2061
SmlI	CTYRAG	2 cut(s)	1328, 1567
SmoI	CTYRAG	2 cut(s)	1328, 1567
SspMI	CTAG	4 cut(s)	161, 1469, 1650, 1953
StyD4I	CCNGG	7 cut(s)	147, 246, 341, 442, 1744, 2338, 2547
StyI	CCWWGG	3 cut(s)	913, 1209, 2671
TaiI	ACGT	1 cut(s)	320
TaqI	TCGA	8 cut(s)	153, 187, 279, 572, 775, 858, 882, 2224
TauI	GCSGC	3 cut(s)	822, 1134, 1566
Tru1I	TTAA	5 cut(s)	29, 1040, 1227, 2175, 2603
Tru9I	TTAA	5 cut(s)	29, 1040, 1227, 2175, 2603
TseFI	GTSAC	2 cut(s)	526, 2264
TseI	GCWGC	6 cut(s)	439, 1205, 1310, 1507, 1784, 2495
Tsp45I	GTSAC	2 cut(s)	526, 2264
TspDTI	ATGAA	6 cut(s)	705, 1230, 1284, 1304, 1404, 1896
TspGWI	ACGGA	2 cut(s)	405, 498
Tth111I	GACNNNGTC	1 cut(s)	13
Van91I	CCANNNNNTGG	1 cut(s)	1447
VpaK11BI	GGWCC	4 cut(s)	244, 392, 1045, 2458
XapI	RAATTY	5 cut(s)	329, 702, 1035, 1217, 2620
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2025
XspI	CTAG	4 cut(s)	161, 1469, 1650, 1953
Zsp2I	ATGCAT	1 cut(s)	1177

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.