Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0011324

Physical Location & Seq

Genomic Coordinates Reverse (-)

22143283 .. 22147162

Gene Structure 3880 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1221700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2682 bp

ATGGCTCCGACGCCGTCTTCTTCCAAATCAAACCACTCCCACACAACCCAGTTCAAGACTCCGCTATCCAAGCACCGCCTCCAGTTCCCCAAAGTGCACCCATCTCCGAACCCAAACTCCGCCGTAAAAGATCCACCAGCGGAGCACCCGGTCGAAGTTCTAGCCCGAATCCGAGACCACCCAGATCGAAAAGAACAACCCGCATCGGTTCTCCAGATCAATCCTCAGAGGCAGAGTATTCGTGTCCGGGCCGATTTTGGGTACCGGGATTTCACGCTCGATGGGGTTTCGTTATCGGAAGAAGACGATTTGGGGACGTTTTACAGAAAATTTGTCCAGTCCAGGATTGACAGTGTGAAATTGGGAGAAAAATGTACGATCATGATGTACGGCCCAACTGGGTCGGGCAAGAGTCACACCATGTTCGGGTGTTGCAAGCAGCCTGGGATCGTTTACAGGTCGTTGAAAGATGTTCTTGGGAATGGGGAAGATGACGGTGGTGCTGCGGTTGGGTCGTTTGTGCAGGTCACTGTTTTGGAGATTTACAATGAGGAGATTTATGATTTGTTGCCGAGCAACAGCGGCGGGGGATTCGGGTATGGATGGCCAAAGGGCAATGGCTCCAATTCTAAGGTTAGGCTTGAAGTTATGGGGAAGAAGGCAAAGAATGCAACCTACATTTCTGGAAATGAAGCTGGGAAAATTTCAAAAGAGATTCAGAAAGTGGAGAAAAGAAGGATTGTTAAGAGCACCCTTTGCAACGATAGAAGTTCTCGAAGCCATTGCATGATAATCCTTGATGTCCCAACAGTGGGAGGACGCTTGATGCTCGTGGACATGGCTGGATCTGAAAATATTGAGCAAGCTGGCCAAATTGGGTTTGATGCAAAAATGCAGACTGCAAAGATCAACCAGGGAAACATAGCTCTGAAAAGGGTGGTTGAATCCATTGCCAATGGTGATTCTCATGTGCCCTTCAGGGACAGCAAACTGACCATGCTTCTACAGGATTCTTTTGAGGACGACAAGACAAAAATCTTAATGGTCCTGTGTGCAAGTCCAGACCCAAAGGAGATACACAAGACAATCTCCACCCTTGAATATGGTGCAAAAGCAAAATGTATTGTCCGCGGACCTCATACGCCAATCAAGGACAAAACTGGGACCGAAGATTCATCTGCAGCTATTTTAGGATCAAGGATTGCTGCCATGGATGAATTTATCCTTAAACTGCAAAGGGAGAATAAGCTCAAAGAGAAAGAGCGGAATGAAGCACACAAGGAGCTAATGAATAAAGAAGAAGAGGTTGCTGCATTGAGGTCTAAACTTGAGCTTGTGGAAGGGAAGGGATCAGGGGCAAAGGAGGAAGAAATCAACTTGAAGGTGAATGAAGTGGCCCAGACTCTGAAAAATGAGTTGGAAAAGAAGTTGGCAGAATGCCAGAGAATGGCGAATGAGTTTGTTGAACTCGAGAGAAGGAGAATGGAAGAAAGGATATTGCAACAGCAGCAGGAAGTTGAAATGCTGAGGCGGCGGTTGGAGGAAATTGAGCTTGAACTGTGCCGCTCAAGCGATGGAAATGGTAAGGAAAGCGGGTGGTCGAAGGAGCCTGATGGAACGCAGTTTGCCAAAAGGTTGTTAGGAATCTATGCCAGTGATGATGCAGGAGGAATGGTAAAGTCAATGGATTTAGACATGGATGATCAAGAAACGTTTACTCGGGAGATCAAATATGTAGGAGTGGTACCTGGGGTTGCTTATCCATCTGACAGCAATAATGTAAACACTGCACACATTGATTCTTTTGAGCCAAAATATGCTGACAGGGTATGCCTGAGCACTGTTTTTGAGGAAGAAGAAATGGAAGAAGAGGAAGGAGCTAACAAAAATGTGGAGGCTGAAGAGGTTGAGAAAGAAGTTATAGAGGAAGAGAGAGTGTGTATGGTTGATGGATCTAGCTTCAGTAGTAATTATAATGTGGACTCCCTGACATCGTTACCTCAGGATTATCAAGATACACCAACTGTGCTTTGGCAAAGCAGAAATCCGGAGGAAGAAGATAACAATGGAAAACCAGATGATAGGCTTGTCAGAATCTGCAACATATTTACACTCTGTGGAGATTACAGAGAGATCTCTCAGCATATCGCACGTCCAGCACCTACTGTTGCATCAGTTAAATCAGAAAATGAGCAGAAACTGGCAGAATCAGAGACAAACAAAAGCCGAGTGTGTGATGAGACAGCATTGGCCTCCAGGGAAAACTACGACCCTTCAAATGAGGGCACTGACTCGGATACTACTGAAGTGTATGTGAAATGGGAAGCTTCAAAAGAAAATCCAGGGAAGTTCATTACAACACTTAAGGTTGTAAAAGACGCAACCCTTGCTGACCTGCGAAAGTTGATTGAAATCTATGTCGGTGCAGAAAATCAAGCATTCACTTTTGTCATGCTTGGAGTTGGGGACCCTAGTGGAGCATCGGTAGAGAAGGAGAAGGAAGCAGCAACTCAGACAACAAAGCTTCCCCTACACAACAATCAATCAAATAGGTACCTGGCCTGCTTGAGGCCACTGAAAGGAATGCAAAGCCCAAGTAGGCTTCTCCCATTAAGTCCCCTCCCATTGAAATCGCTGGAAAACAGGCTACACTCCGACTTCCTGCTTCTCAGTCCATGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005856 GO:0005871 GO:0005875 GO:0006810 GO:0006928 GO:0007017 GO:0007018 GO:0008017 GO:0008092 GO:0008150 GO:0008574 GO:0009524 GO:0009987 GO:0010970 GO:0015630 GO:0015631 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0030705 GO:0032991 GO:0042623 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0046907 GO:0047496 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051648 GO:0051649 GO:0051650 GO:0051656 GO:0072384 GO:0099111 GO:0099518 GO:1990939

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin

Protein Analysis

894

Amino Acids

99.6

Weight (kDa)

5.37

Isoelectric Point (pI)

52.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Kinesin	PF00225	76 - 375	7.5e-60	Kinesin motor domain
Microtub_bd	PF16796	86 - 190	2.4e-08	Microtubule binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011324)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G14330
fragaria_vesca	FvH4_4g04000 FvH4_4g04000
malus_domestica	MD13G1216300.v1.1 MD16G1221700.v1.1
prunus_persica	Prupe.1G042100_v2.0.a1 Prupe.4G184200_v2.0.a1
pyrus_communis	pycom13g18980 pycom16g18590
rosa_chinensis	RchiOBHm_Chr4g0394251
rosa_laevigata	RLG00000009711
rosa_multiflora	Rmu_sc0007246.1_g000005 Rmu_ssc0000473.1_g000031
rosa_roxburghii	Rroxscaffold_5G00339300
rosa_rugosa	Rorug03G0368300.1
rosa_samantha	Rh4AG051100 Rh4BG048100 Rh4CG054800 Rh4DG047800
rosa_wichuraiana	Rw4G004070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1974
Acc36I	ACCTGC	2 cut(s)	514, 2403
Acc65I	GGTACC	3 cut(s)	261, 1744, 2553
AccB1I	GGYRCC	3 cut(s)	261, 1744, 2553
AccB7I	CCANNNNNTGG	1 cut(s)	1447
AccBSI	CCGCTC	2 cut(s)	1264, 1566
AccII	CGCG	1 cut(s)	1131
AccIII	TCCGGA	1 cut(s)	2047
AclI	AACGTT	1 cut(s)	1712
AclWI	GGATC	6 cut(s)	125, 455, 853, 1201, 1357, 1960
AcoI	YGGCCR	2 cut(s)	605, 868
AcsI	RAATTY	3 cut(s)	329, 702, 1217
AcuI	CTGAAG	4 cut(s)	961, 1920, 1945, 2325
AcyI	GRCGYC	1 cut(s)	11
AdeI	CACNNNGTG	1 cut(s)	2117
AfaI	GTAC	5 cut(s)	263, 376, 389, 1746, 2555
AfiI	CCNNNNNNNGG	7 cut(s)	258, 426, 811, 812, 1447, 2568, 2579
AflII	CTTAAG	1 cut(s)	2363
AjiI	CACGTC	1 cut(s)	2153
AjnI	CCWGG	7 cut(s)	341, 442, 912, 1747, 2255, 2341, 2556
AjuI	GAANNNNNNNTTGG	2 cut(s)	1400, 1432
AloI	GAACNNNNNNTCC	2 cut(s)	101, 133
Alw21I	GWGCWC	4 cut(s)	99, 147, 752, 1841
Alw26I	GTCTC	3 cut(s)	168, 2207, 2234
Alw44I	GTGCAC	1 cut(s)	95
AlwI	GGATC	6 cut(s)	125, 455, 853, 1201, 1357, 1960
AlwNI	CAGNNNCTG	3 cut(s)	1405, 2097, 2200
Ama87I	CYCGRG	2 cut(s)	1469, 1719
Aor13HI	TCCGGA	1 cut(s)	2047
AoxI	GGCC	8 cut(s)	249, 391, 605, 868, 1395, 2250, 2559, 2570
ApaLI	GTGCAC	1 cut(s)	95
ApeKI	GCWGC	7 cut(s)	439, 503, 1181, 1205, 1310, 1507, 2504
ApoI	RAATTY	3 cut(s)	329, 702, 1217
Asp718I	GGTACC	3 cut(s)	261, 1744, 2553
AspS9I	GGNCC	7 cut(s)	249, 392, 1045, 1133, 1164, 1396, 2467
AsuC2I	CCSGG	3 cut(s)	149, 248, 266
AsuHPI	GGTGA	2 cut(s)	971, 1396
AvaI	CYCGRG	2 cut(s)	1469, 1719
AvaII	GGWCC	4 cut(s)	1045, 1133, 1164, 2467
AxyI	CCTNAGG	1 cut(s)	2001
BaeGI	GKGCMC	3 cut(s)	99, 975, 2288
BalI	TGGCCA	2 cut(s)	607, 870
BanI	GGYRCC	3 cut(s)	261, 1744, 2553
BauI	CACGAG	1 cut(s)	830
BbsI	GAAGAC	2 cut(s)	9, 309
Bbv12I	GWGCWC	4 cut(s)	99, 147, 752, 1841
BbvCI	CCTCAGC	1 cut(s)	1526
BbvI	GCAGC	7 cut(s)	451, 490, 1192, 1193, 1297, 1519, 2516
BccI	CCATC	7 cut(s)	109, 275, 597, 1568, 1607, 1771, 1943
BceAI	ACGGC	2 cut(s)	107, 406
BcgI	CGANNNNNNTGC	4 cut(s)	221, 255, 765, 799
BciT130I	CCWGG	7 cut(s)	343, 444, 914, 1749, 2257, 2343, 2558
BciVI	GTATCC	1 cut(s)	2290
BclI	TGATCA	1 cut(s)	1702
BcnI	CCSGG	3 cut(s)	149, 248, 266
BcoDI	GTCTC	3 cut(s)	168, 2207, 2234
BfaI	CTAG	3 cut(s)	161, 1956, 2472
BfmI	CTRYAG	2 cut(s)	1004, 1179
BfrI	CTTAAG	1 cut(s)	2363
BfuAI	ACCTGC	2 cut(s)	514, 2403
BfuI	GTATCC	1 cut(s)	2290
BglII	AGATCT	1 cut(s)	2133
Bme18I	GGWCC	4 cut(s)	1045, 1133, 1164, 2467
BmeT110I	CYCGRG	2 cut(s)	1469, 1719
BmgBI	CACGTC	1 cut(s)	2153
BmgT120I	GGNCC	7 cut(s)	249, 392, 1045, 1133, 1164, 1396, 2467
BmiI	GGNNCC	9 cut(s)	6, 263, 622, 1165, 1608, 1746, 2468, 2469, 2555
BmrI	ACTGGG	3 cut(s)	43, 408, 1170
BmsI	GCATC	6 cut(s)	212, 816, 874, 1651, 2180, 2489
BmuI	ACTGGG	3 cut(s)	43, 408, 1170
BpiI	GAAGAC	2 cut(s)	9, 309
BpmI	CTGGAG	3 cut(s)	65, 197, 2239
Bpu10I	CCTNAGC	2 cut(s)	1526, 1835
BpuEI	CTTGAG	3 cut(s)	1349, 1552, 2587
BpuMI	CCSGG	3 cut(s)	149, 248, 266
BsaBI	GATNNNNATC	1 cut(s)	2411
BsaHI	GRCGYC	1 cut(s)	11
BsaI	GGTCTC	1 cut(s)	168
BsaJI	CCNNGG	8 cut(s)	443, 913, 1129, 1209, 1748, 2256, 2342, 2675
BsaWI	WCCGGW	1 cut(s)	2047
BsaXI	ACNNNNNCTCC	6 cut(s)	101, 131, 1659, 1689, 2469, 2499
Bsc4I	CCNNNNNNNGG	7 cut(s)	258, 426, 811, 812, 1447, 2568, 2579
Bse1I	ACTGG	7 cut(s)	49, 82, 337, 403, 1165, 1653, 2205
Bse21I	CCTNAGG	1 cut(s)	2001
Bse3DI	GCAATG	3 cut(s)	622, 781, 948
Bse8I	GATNNNNATC	1 cut(s)	2411
BseAI	TCCGGA	1 cut(s)	2047
BseBI	CCWGG	7 cut(s)	343, 444, 914, 1749, 2257, 2343, 2558
BseDI	CCNNGG	8 cut(s)	443, 913, 1129, 1209, 1748, 2256, 2342, 2675
BseGI	GGATG	3 cut(s)	608, 1219, 1705
BseJI	GATNNNNATC	1 cut(s)	2411
BseLI	CCNNNNNNNGG	7 cut(s)	258, 426, 811, 812, 1447, 2568, 2579
BseMI	GCAATG	3 cut(s)	622, 781, 948
BseMII	CTCAG	6 cut(s)	239, 1517, 1826, 2015, 2153, 2525
BseNI	ACTGG	7 cut(s)	49, 82, 337, 403, 1165, 1653, 2205
BseRI	GAGGAG	1 cut(s)	566
BseSI	GKGCMC	3 cut(s)	99, 975, 2288
BseXI	GCAGC	7 cut(s)	451, 490, 1192, 1193, 1297, 1519, 2516
BseYI	CCCAGC	1 cut(s)	695
BsgI	GTGCAG	3 cut(s)	542, 1773, 2445
Bsh1236I	CGCG	1 cut(s)	1131
Bsh1285I	CGRYCG	1 cut(s)	153
BshFI	GGCC	8 cut(s)	251, 393, 607, 870, 1397, 2252, 2561, 2572
BshNI	GGYRCC	3 cut(s)	261, 1744, 2553
BsiEI	CGRYCG	1 cut(s)	153
BsiHKAI	GWGCWC	4 cut(s)	99, 147, 752, 1841
BsiHKCI	CYCGRG	2 cut(s)	1469, 1719
BsiSI	CCGG	4 cut(s)	149, 247, 265, 2048
BslFI	GGGAC	6 cut(s)	328, 788, 995, 1177, 2480, 2601
BslI	CCNNNNNNNGG	7 cut(s)	258, 426, 811, 812, 1447, 2568, 2579
BsmAI	GTCTC	3 cut(s)	168, 2207, 2234
BsmFI	GGGAC	6 cut(s)	328, 788, 995, 1177, 2480, 2601
BsmI	GAATGC	4 cut(s)	673, 1442, 2438, 2589
BsnI	GGCC	8 cut(s)	251, 393, 607, 870, 1397, 2252, 2561, 2572
Bso31I	GGTCTC	1 cut(s)	168
BsoBI	CYCGRG	2 cut(s)	1469, 1719
Bsp1286I	GDGCHC	6 cut(s)	99, 147, 752, 975, 1841, 2288
Bsp13I	TCCGGA	1 cut(s)	2047
Bsp19I	CCATGG	2 cut(s)	1209, 2675
BspANI	GGCC	8 cut(s)	251, 393, 607, 870, 1397, 2252, 2561, 2572
BspCNI	CTCAG	7 cut(s)	238, 1518, 1827, 2014, 2152, 2524, 2682
BspEI	TCCGGA	1 cut(s)	2047
BspFNI	CGCG	1 cut(s)	1131
BspHI	TCATGA	1 cut(s)	381
BspLI	GGNNCC	9 cut(s)	6, 263, 622, 1165, 1608, 1746, 2468, 2469, 2555
BspMAI	CTGCAG	1 cut(s)	1183
BspMI	ACCTGC	2 cut(s)	514, 2403
BspPI	GGATC	6 cut(s)	125, 455, 853, 1201, 1357, 1960
BspT107I	GGYRCC	3 cut(s)	261, 1744, 2553
BspTI	CTTAAG	1 cut(s)	2363
BspTNI	GGTCTC	1 cut(s)	168
BsrBI	CCGCTC	2 cut(s)	1264, 1566
BsrDI	GCAATG	3 cut(s)	622, 781, 948
BsrI	ACTGG	7 cut(s)	49, 82, 337, 403, 1165, 1653, 2205
BssECI	CCNNGG	8 cut(s)	443, 913, 1129, 1209, 1748, 2256, 2342, 2675
BssNI	GRCGYC	1 cut(s)	11
BssSI	CACGAG	1 cut(s)	830
BssT1I	CCWWGG	2 cut(s)	1209, 2675
Bst2BI	CACGAG	1 cut(s)	830
Bst2UI	CCWGG	7 cut(s)	343, 444, 914, 1749, 2257, 2343, 2558
Bst4CI	ACNGT	8 cut(s)	353, 497, 532, 811, 1560, 1843, 2026, 2167
Bst6I	CTCTTC	4 cut(s)	1296, 1863, 1896, 1923
BstACI	GRCGYC	1 cut(s)	11
BstAFI	CTTAAG	1 cut(s)	2363
BstAPI	GCANNNNNTGC	3 cut(s)	668, 756, 2387
BstC8I	GCNNGC	4 cut(s)	437, 864, 868, 2563
BstDEI	CTNAG	8 cut(s)	225, 630, 1526, 1835, 2001, 2139, 2511, 2669
BstDSI	CCRYGG	3 cut(s)	1129, 1209, 2675
BstENI	CCTNNNNNAGG	1 cut(s)	2566
BstF5I	GGATG	3 cut(s)	608, 1219, 1705
BstFNI	CGCG	1 cut(s)	1131
BstMAI	GTCTC	3 cut(s)	168, 2207, 2234
BstMCI	CGRYCG	1 cut(s)	153
BstMWI	GCNNNNNNNGC	9 cut(s)	70, 582, 668, 756, 1507, 1531, 1569, 2156, 2387
BstNI	CCWGG	7 cut(s)	343, 444, 914, 1749, 2257, 2343, 2558
BstSFI	CTRYAG	2 cut(s)	1004, 1179
BstSLI	GKGCMC	3 cut(s)	99, 975, 2288
BstUI	CGCG	1 cut(s)	1131
BstV1I	GCAGC	7 cut(s)	451, 490, 1192, 1193, 1297, 1519, 2516
BstV2I	GAAGAC	2 cut(s)	9, 309
BstX2I	RGATCY	4 cut(s)	130, 845, 1952, 2133
BstYI	RGATCY	4 cut(s)	130, 845, 1952, 2133
Bsu36I	CCTNAGG	1 cut(s)	2001
BsuI	GTATCC	1 cut(s)	2290
BsuRI	GGCC	8 cut(s)	251, 393, 607, 870, 1397, 2252, 2561, 2572
BtgI	CCRYGG	3 cut(s)	1129, 1209, 2675
BtgZI	GCGATG	1 cut(s)	1587
BtrI	CACGTC	1 cut(s)	2153
BtsCI	GGATG	3 cut(s)	608, 1219, 1705
BtsI	GCAGTG	1 cut(s)	1785
BtsIMutI	CAGTG	8 cut(s)	358, 528, 816, 1660, 1785, 1839, 2286, 2573
BveI	ACCTGC	2 cut(s)	514, 2403
Cac8I	GCNNGC	4 cut(s)	437, 864, 868, 2563
CaiI	CAGNNNCTG	3 cut(s)	1405, 2097, 2200
CciI	TCATGA	1 cut(s)	381
Cfr13I	GGNCC	7 cut(s)	249, 392, 1045, 1133, 1164, 1396, 2467
Cfr42I	CCGCGG	1 cut(s)	1132
CseI	GACGC	3 cut(s)	19, 828, 2387
Csp6I	GTAC	5 cut(s)	262, 375, 388, 1745, 2554
CviQI	GTAC	5 cut(s)	262, 375, 388, 1745, 2554
DdeI	CTNAG	8 cut(s)	225, 630, 1526, 1835, 2001, 2139, 2511, 2669
DraIII	CACNNNGTG	1 cut(s)	2117
EaeI	YGGCCR	2 cut(s)	605, 868
Eam1104I	CTCTTC	4 cut(s)	1296, 1863, 1896, 1923
EarI	CTCTTC	4 cut(s)	1296, 1863, 1896, 1923
EciI	GGCGGA	1 cut(s)	109
Eco130I	CCWWGG	2 cut(s)	1209, 2675
Eco31I	GGTCTC	1 cut(s)	168
Eco47I	GGWCC	4 cut(s)	1045, 1133, 1164, 2467
Eco57I	CTGAAG	4 cut(s)	961, 1920, 1945, 2325
Eco81I	CCTNAGG	1 cut(s)	2001
Eco88I	CYCGRG	2 cut(s)	1469, 1719
EcoNI	CCTNNNNNAGG	1 cut(s)	2566
EcoO109I	RGGNCCY	1 cut(s)	2467
EcoRII	CCWGG	7 cut(s)	341, 442, 912, 1747, 2255, 2341, 2556
EcoT14I	CCWWGG	2 cut(s)	1209, 2675
ErhI	CCWWGG	2 cut(s)	1209, 2675
FalI	AAGNNNNNCTT	2 cut(s)	459, 491
FaqI	GGGAC	6 cut(s)	328, 788, 995, 1177, 2480, 2601
FauI	CCCGC	3 cut(s)	208, 578, 1586
FbaI	TGATCA	1 cut(s)	1702
FokI	GGATG	3 cut(s)	615, 1226, 1712
FspBI	CTAG	3 cut(s)	161, 1956, 2472
GsaI	CCCAGC	1 cut(s)	699
GsuI	CTGGAG	3 cut(s)	65, 197, 2239
HaeIII	GGCC	8 cut(s)	251, 393, 607, 870, 1397, 2252, 2561, 2572
HapII	CCGG	4 cut(s)	149, 247, 265, 2048
HgaI	GACGC	3 cut(s)	19, 828, 2387
Hin1I	GRCGYC	1 cut(s)	11
HindIII	AAGCTT	2 cut(s)	2325, 2522
HpaII	CCGG	4 cut(s)	149, 247, 265, 2048
HphI	GGTGA	2 cut(s)	971, 1396
Hpy166II	GTNNAC	6 cut(s)	97, 454, 835, 1716, 1783, 1981
Hpy8I	GTNNAC	6 cut(s)	97, 454, 835, 1716, 1783, 1981
Hpy99I	CGWCG	1 cut(s)	13
HpyCH4III	ACNGT	8 cut(s)	353, 497, 532, 811, 1560, 1843, 2026, 2167
HpyCH4IV	ACGT	3 cut(s)	317, 1712, 2152
HpyF10VI	GCNNNNNNNGC	9 cut(s)	70, 582, 668, 756, 1507, 1531, 1569, 2156, 2387
HpyF3I	CTNAG	8 cut(s)	225, 630, 1526, 1835, 2001, 2139, 2511, 2669
HpySE526I	ACGT	3 cut(s)	317, 1712, 2152
Hsp92I	GRCGYC	1 cut(s)	11
KflI	GGGWCCC	1 cut(s)	2467
Kpn2I	TCCGGA	1 cut(s)	2047
KpnI	GGTACC	3 cut(s)	265, 1748, 2557
Ksp22I	TGATCA	1 cut(s)	1702
KspI	CCGCGG	1 cut(s)	1132
LmnI	GCTCC	7 cut(s)	10, 142, 626, 1282, 1606, 1877, 2477
Lsp1109I	GCAGC	7 cut(s)	451, 490, 1192, 1193, 1297, 1519, 2516
LweI	GCATC	6 cut(s)	212, 816, 874, 1651, 2180, 2489
MaeI	CTAG	3 cut(s)	161, 1956, 2472
MaeII	ACGT	3 cut(s)	317, 1712, 2152
MaeIII	GTNAC	3 cut(s)	413, 526, 1995
MbiI	CCGCTC	2 cut(s)	1264, 1566
MflI	RGATCY	4 cut(s)	130, 845, 1952, 2133
MhlI	GDGCHC	6 cut(s)	99, 147, 752, 975, 1841, 2288
MlsI	TGGCCA	2 cut(s)	607, 870
MluCI	AATT	8 cut(s)	329, 359, 625, 702, 873, 1217, 1545, 1969
MluNI	TGGCCA	2 cut(s)	607, 870
MlyI	GAGTC	5 cut(s)	52, 421, 1396, 1976, 2285
MmeI	TCCRAC	4 cut(s)	32, 1398, 1518, 2679
Mox20I	TGGCCA	2 cut(s)	607, 870
MroI	TCCGGA	1 cut(s)	2047
MscI	TGGCCA	2 cut(s)	607, 870
MseI	TTAA	6 cut(s)	744, 1040, 1227, 2178, 2364, 2612
Msp20I	TGGCCA	2 cut(s)	607, 870
MspA1I	CMGCKG	3 cut(s)	140, 582, 1131
MspCI	CTTAAG	1 cut(s)	2363
MspI	CCGG	4 cut(s)	149, 247, 265, 2048
Mva1269I	GAATGC	4 cut(s)	673, 1442, 2438, 2589
MvaI	CCWGG	7 cut(s)	343, 444, 914, 1749, 2257, 2343, 2558
MvnI	CGCG	1 cut(s)	1131
MwoI	GCNNNNNNNGC	9 cut(s)	70, 582, 668, 756, 1507, 1531, 1569, 2156, 2387
NciI	CCSGG	3 cut(s)	149, 248, 266
NcoI	CCATGG	2 cut(s)	1209, 2675
NlaIV	GGNNCC	9 cut(s)	6, 263, 622, 1165, 1608, 1746, 2468, 2469, 2555
NmeAIII	GCCGAG	2 cut(s)	597, 2252
NmuCI	GTSAC	2 cut(s)	413, 526
PaeR7I	CTCGAG	1 cut(s)	1469
PagI	TCATGA	1 cut(s)	381
PctI	GAATGC	4 cut(s)	673, 1442, 2438, 2589
PflFI	GACNNNGTC	1 cut(s)	13
PflMI	CCANNNNNTGG	1 cut(s)	1447
PfoI	TCCNGGA	1 cut(s)	341
PleI	GAGTC	5 cut(s)	52, 420, 1396, 1976, 2285
PpsI	GAGTC	5 cut(s)	52, 420, 1396, 1976, 2285
PpuMI	RGGWCCY	1 cut(s)	2467
PsiI	TTATAA	1 cut(s)	1974
Psp1406I	AACGTT	1 cut(s)	1712
Psp5II	RGGWCCY	1 cut(s)	2467
Psp6I	CCWGG	7 cut(s)	341, 442, 912, 1747, 2255, 2341, 2556
PspFI	CCCAGC	1 cut(s)	695
PspGI	CCWGG	7 cut(s)	341, 442, 912, 1747, 2255, 2341, 2556
PspN4I	GGNNCC	9 cut(s)	6, 263, 622, 1165, 1608, 1746, 2468, 2469, 2555
PspPI	GGNCC	7 cut(s)	249, 392, 1045, 1133, 1164, 1396, 2467
PspPPI	RGGWCCY	1 cut(s)	2467
PstI	CTGCAG	1 cut(s)	1183
PstNI	CAGNNNCTG	3 cut(s)	1405, 2097, 2200
PsuI	RGATCY	4 cut(s)	130, 845, 1952, 2133
PsyI	GACNNNGTC	1 cut(s)	13
RsaI	GTAC	5 cut(s)	263, 376, 389, 1746, 2555
RsaNI	GTAC	5 cut(s)	262, 375, 388, 1745, 2554
SacII	CCGCGG	1 cut(s)	1132
SaqAI	TTAA	6 cut(s)	744, 1040, 1227, 2178, 2364, 2612
Sau96I	GGNCC	7 cut(s)	249, 392, 1045, 1133, 1164, 1396, 2467
SchI	GAGTC	5 cut(s)	52, 421, 1396, 1976, 2285
SduI	GDGCHC	6 cut(s)	99, 147, 752, 975, 1841, 2288
SfaNI	GCATC	6 cut(s)	212, 816, 874, 1651, 2180, 2489
SfcI	CTRYAG	2 cut(s)	1004, 1179
Sfr274I	CTCGAG	1 cut(s)	1469
Sfr303I	CCGCGG	1 cut(s)	1132
SgrBI	CCGCGG	1 cut(s)	1132
SinI	GGWCC	4 cut(s)	1045, 1133, 1164, 2467
SlaI	CTCGAG	1 cut(s)	1469
SmlI	CTYRAG	5 cut(s)	1328, 1469, 1567, 2363, 2566
SmoI	CTYRAG	5 cut(s)	1328, 1469, 1567, 2363, 2566
Sse9I	AATT	8 cut(s)	329, 359, 625, 702, 873, 1217, 1545, 1969
SspI	AATATT	1 cut(s)	856
SspMI	CTAG	3 cut(s)	161, 1956, 2472
StyI	CCWWGG	2 cut(s)	1209, 2675
TaaI	ACNGT	8 cut(s)	353, 497, 532, 811, 1560, 1843, 2026, 2167
TaiI	ACGT	3 cut(s)	320, 1715, 2155
TaqI	TCGA	6 cut(s)	153, 187, 279, 775, 1470, 1601
TaqII	GACCGA	1 cut(s)	1181
TasI	AATT	8 cut(s)	329, 359, 625, 702, 873, 1217, 1545, 1969
TauI	GCSGC	3 cut(s)	585, 1534, 1566
Tru1I	TTAA	6 cut(s)	744, 1040, 1227, 2178, 2364, 2612
Tru9I	TTAA	6 cut(s)	744, 1040, 1227, 2178, 2364, 2612
TscAI	CASTG	8 cut(s)	358, 535, 816, 1660, 1792, 1846, 2293, 2580
TseFI	GTSAC	2 cut(s)	413, 526
TseI	GCWGC	7 cut(s)	439, 503, 1181, 1205, 1310, 1507, 2504
Tsp45I	GTSAC	2 cut(s)	413, 526
TspDTI	ATGAA	7 cut(s)	705, 1164, 1230, 1284, 1304, 1404, 2341
TspRI	CASTG	8 cut(s)	358, 535, 816, 1660, 1792, 1846, 2293, 2580
Tth111I	GACNNNGTC	1 cut(s)	13
Van91I	CCANNNNNTGG	1 cut(s)	1447
Vha464I	CTTAAG	1 cut(s)	2363
VneI	GTGCAC	1 cut(s)	95
VpaK11BI	GGWCC	4 cut(s)	1045, 1133, 1164, 2467
XagI	CCTNNNNNAGG	1 cut(s)	2566
XapI	RAATTY	3 cut(s)	329, 702, 1217
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2028
XhoI	CTCGAG	1 cut(s)	1469
XspI	CTAG	3 cut(s)	161, 1956, 2472

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.