Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0009677

Physical Location & Seq

Genomic Coordinates Forward (+)

21465401 .. 21470592

Gene Structure 5192 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1221800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1425 bp

ATGGCCACCCCCTATGCCTTTCCCGTCACTGCTGCTCAGGTTGGGACGTACTTTGTGACACAGTACTACCAAGTGCTTCAGCAGCAGCCGGACTTCGTGCACCAGTTCTACTCCGAGGCCAGCACTATGGTTCGTGCCGATGGGAATTCCAGAGCCACCGCCGTCGCAATGCAGCAAATCCATGATCTTGTTATGTCACTTAATCTGACTGGATTTGAAATCAACAAAGCAAATTCCTTGGAATCCTGGAATGGAGGTGTTCTTGTGCTGGTTGAAGGCTACCTTGAAATGAAAACTTTCACTGGGAAGAGGAAATTTGCTCAAACGTTTTTCCTTGCACCTCAGGAGAGAGGTTTTTTCGTGCTGAATGATATATTTCACTTCGTTGATGAGGAACCGATTCATCAACATCCAGCAGTCTTATTAGCCCAAAACAATCTCGATGCCAAGCTAAGTGCTTCTGCTACAATTCCGCATCCAGTGGCTAACTATTATCTGGGTGAAGAAATTCAGACAAGAGACTTTGTGGCTCCTGCTGTCAAAGAAAATGGACCAGTTGAACGTTATGGTTTTGCCGAGCAGCAGTTGCAGCAAGTTGTTGAAACTGAGAACTTGTTGGATGATGGTGCTGTAGAACAATCAAATGGTTCACTTCAAAGCACGCCGAATACCGTACAAGATCACCTGCATGCATCTGTTGAGGAATCTATTGGAGAGCCCCAAAAGCAGACCTATGCTTCTATATTACGCGTTGCTAAAGGACAACCTGTGCCTTCTGTAACTCCCCACCATTCATCTAACAAAAGTGCACCAATGGCTTCAGAGTGGAATCACTATCCTAAGTCCTCTGCTCAGCAGCCCATTTCACCATCTAATACATTTGAAGGGCCTGTAGAAGAGACAGCAGATGAAGTTGCTGCCCCAGAAGATGAAGGCGAAATAAAGTCTGTTTATGTAAGAAACTTGCCGACTACTGTTTCCCCGTCTGAAGTTGAGGAGGAATTTGTGAATTTTGGAAAACTCAAGCAGCCTGAAGGAGTGGTTATTAGAAGTCGCAAGGATGTTGGTGTTTGCTATGCGTTTGTTGAATTTGAAGATATTACCGGTGTGCAGAATGCCGTCAAGGCTGGTAGTGTTCAGATTGCTGGACGACAAGTATACATTGAAGAGCGGAGACCAAACAGCAACATCCCGTCTCGAGTGGGAAGAAGGGGAAGAGGAAGGGGCAGCTACCAAGCAGAGGCACCGAGAGGTCATTTTGGCTCGCGGGGTTTTTGGAGGGGAGGTGGCTATGATGGAGGTGACCAGGAGTACAGTCGACCGAGGGGAAATGGCTACTATCGGTCAAGTCCTCGACAAGACACAGGGAACTCGGGTTATCAATCATCAAGAAGCGGACAAAATTCATCGGAGTTCACTAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006508 GO:0006807 GO:0006914 GO:0008150 GO:0008152 GO:0009056 GO:0009987 GO:0010494 GO:0016236 GO:0016579 GO:0019538 GO:0032386 GO:0032879 GO:0032991 GO:0034517 GO:0035770 GO:0035973 GO:0036211 GO:0036464 GO:0043021 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043412 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044877 GO:0050789 GO:0050794 GO:0051049 GO:0060341 GO:0060627 GO:0060628 GO:0061912 GO:0061919 GO:0065007 GO:0070646 GO:0070647 GO:0071704 GO:0097159 GO:1901363 GO:1901564 GO:1905538 GO:1990861 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NTF2 RRM_1

Protein Analysis

475

Amino Acids

52.36

Weight (kDa)

5.34

Isoelectric Point (pI)

55.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NTF2	PF02136	14 - 128	1.5e-24	Nuclear transport factor 2 (NTF2) domain
RRM_1	PF00076	317 - 382	5.8e-07	RNA recognition motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009677)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g04660 FvH4_4g04660
malus_domestica	MD13G1221800.v1.1 MD16G1226700.v1.1
prunus_persica	Prupe.1G048900_v2.0.a1 Prupe.1G048900_v2.0.a1
pyrus_communis	pycom13g19610 pycom16g18980
rosa_chinensis	RchiOBHm_Chr4g0395471
rosa_laevigata	RLG00000009620
rosa_multiflora	Rmu_sc0001951.1_g000003 Rmu_sc0011701.1_g000032
rosa_roxburghii	Rroxscaffold_5G00340410
rosa_rugosa	Rorug03G0356000 Rorug03G0356000 Rorug03G0356000
rosa_samantha	Rh4AG060400 Rh4AG062100 Rh4BG059300 Rh4CG065000 Rh4DG055300
rosa_wichuraiana	Rw4G005070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	693
Acc36I	ACCTGC	1 cut(s)	693
AccB1I	GGYRCC	1 cut(s)	1243
AccBSI	CCGCTC	1 cut(s)	1171
AccI	GTMKAC	2 cut(s)	1158, 1318
AccII	CGCG	2 cut(s)	750, 1267
AciI	CCGC	5 cut(s)	159, 473, 1171, 1267, 1395
AclI	AACGTT	2 cut(s)	326, 562
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	8 cut(s)	145, 232, 314, 507, 1001, 1009, 1088, 1402
AcuI	CTGAAG	4 cut(s)	62, 804, 1008, 1053
AfaI	GTAC	4 cut(s)	50, 65, 675, 1313
AfiI	CCNNNNNNNGG	1 cut(s)	1240
AflIII	ACRYGT	1 cut(s)	748
AgeI	ACCGGT	1 cut(s)	1103
AjnI	CCWGG	2 cut(s)	245, 1305
AluBI	AGCT	2 cut(s)	451, 1230
AluI	AGCT	2 cut(s)	451, 1230
Alw21I	GWGCWC	2 cut(s)	102, 811
Alw26I	GTCTC	4 cut(s)	513, 893, 1168, 1200
Alw44I	GTGCAC	2 cut(s)	98, 807
Ama87I	CYCGRG	2 cut(s)	1197, 1372
AoxI	GGCC	3 cut(s)	3, 117, 887
ApaLI	GTGCAC	2 cut(s)	98, 807
ApoI	RAATTY	8 cut(s)	145, 232, 314, 507, 1001, 1009, 1088, 1402
AsiGI	ACCGGT	1 cut(s)	1103
Asp700I	GAANNNNTTC	3 cut(s)	296, 399, 507
AspS9I	GGNCC	2 cut(s)	551, 887
AsuHPI	GGTGA	4 cut(s)	512, 674, 858, 1313
AvaI	CYCGRG	2 cut(s)	1197, 1372
AvaII	GGWCC	1 cut(s)	551
AxyI	CCTNAGG	1 cut(s)	342
BaeGI	GKGCMC	2 cut(s)	102, 811
BalI	TGGCCA	1 cut(s)	5
BanI	GGYRCC	1 cut(s)	1243
BanII	GRGCYC	1 cut(s)	720
Bbv12I	GWGCWC	2 cut(s)	102, 811
BccI	CCATC	4 cut(s)	134, 617, 877, 1289
BceAI	ACGGC	2 cut(s)	146, 1103
BciT130I	CCWGG	2 cut(s)	247, 1307
BcoDI	GTCTC	4 cut(s)	513, 893, 1168, 1200
BfmI	CTRYAG	2 cut(s)	630, 891
BfuAI	ACCTGC	1 cut(s)	693
BglI	GCCNNNNNGGC	1 cut(s)	1124
BlpI	GCTNAGC	1 cut(s)	852
BmcAI	AGTACT	1 cut(s)	65
Bme1390I	CCNGG	2 cut(s)	247, 1307
Bme18I	GGWCC	1 cut(s)	551
BmeT110I	CYCGRG	2 cut(s)	1197, 1372
BmgT120I	GGNCC	2 cut(s)	551, 887
BmiI	GGNNCC	3 cut(s)	396, 531, 1245
BmrFI	CCNGG	2 cut(s)	247, 1307
BmrI	ACTGGG	1 cut(s)	312
BmsI	GCATC	3 cut(s)	433, 484, 701
BmuI	ACTGGG	1 cut(s)	312
Bpu10I	CCTNAGC	1 cut(s)	36
Bpu1102I	GCTNAGC	1 cut(s)	852
BpuEI	CTTGAG	1 cut(s)	1007
BsaI	GGTCTC	1 cut(s)	1168
BsaJI	CCNNGG	3 cut(s)	114, 237, 1322
BsaWI	WCCGGW	1 cut(s)	1103
BsaXI	ACNNNNNCTCC	2 cut(s)	1270, 1300
Bsc4I	CCNNNNNNNGG	1 cut(s)	1240
Bse118I	RCCGGY	1 cut(s)	1103
Bse1I	ACTGG	5 cut(s)	103, 214, 307, 479, 554
Bse21I	CCTNAGG	1 cut(s)	342
Bse3DI	GCAATG	1 cut(s)	174
BseBI	CCWGG	2 cut(s)	247, 1307
BseDI	CCNNGG	3 cut(s)	114, 237, 1322
BseGI	GGATG	5 cut(s)	409, 475, 625, 1066, 1188
BseLI	CCNNNNNNNGG	1 cut(s)	1240
BseMI	GCAATG	1 cut(s)	174
BseMII	CTCAG	4 cut(s)	50, 356, 597, 866
BseNI	ACTGG	5 cut(s)	103, 214, 307, 479, 554
BseRI	GAGGAG	1 cut(s)	1010
BseSI	GKGCMC	2 cut(s)	102, 811
BsgI	GTGCAG	1 cut(s)	1130
Bsh1236I	CGCG	2 cut(s)	750, 1267
Bsh1285I	CGRYCG	1 cut(s)	1322
BshFI	GGCC	3 cut(s)	5, 119, 889
BshNI	GGYRCC	1 cut(s)	1243
BshTI	ACCGGT	1 cut(s)	1103
BsiEI	CGRYCG	1 cut(s)	1322
BsiHKAI	GWGCWC	2 cut(s)	102, 811
BsiHKCI	CYCGRG	2 cut(s)	1197, 1372
BsiSI	CCGG	2 cut(s)	89, 1104
BslFI	GGGAC	1 cut(s)	58
BslI	CCNNNNNNNGG	1 cut(s)	1240
BsmAI	GTCTC	4 cut(s)	513, 893, 1168, 1200
BsmBI	CGTCTC	1 cut(s)	1200
BsmFI	GGGAC	1 cut(s)	58
BsmI	GAATGC	1 cut(s)	1120
BsnI	GGCC	3 cut(s)	5, 119, 889
Bso31I	GGTCTC	1 cut(s)	1168
BsoBI	CYCGRG	2 cut(s)	1197, 1372
Bsp1286I	GDGCHC	3 cut(s)	102, 720, 811
Bsp143I	GATC	2 cut(s)	184, 679
Bsp1720I	GCTNAGC	1 cut(s)	852
BspACI	CCGC	5 cut(s)	159, 473, 1171, 1267, 1395
BspANI	GGCC	3 cut(s)	5, 119, 889
BspCNI	CTCAG	4 cut(s)	49, 355, 598, 865
BspFNI	CGCG	2 cut(s)	750, 1267
BspLI	GGNNCC	3 cut(s)	396, 531, 1245
BspMI	ACCTGC	1 cut(s)	693
BspQI	GCTCTTC	1 cut(s)	1161
BspT107I	GGYRCC	1 cut(s)	1243
BspTNI	GGTCTC	1 cut(s)	1168
BsrBI	CCGCTC	1 cut(s)	1171
BsrDI	GCAATG	1 cut(s)	174
BsrFI	RCCGGY	1 cut(s)	1103
BsrI	ACTGG	5 cut(s)	103, 214, 307, 479, 554
BssAI	RCCGGY	1 cut(s)	1103
BssECI	CCNNGG	3 cut(s)	114, 237, 1322
BssMI	GATC	2 cut(s)	184, 679
BssNAI	GTATAC	1 cut(s)	1159
BssT1I	CCWWGG	1 cut(s)	237
Bst1107I	GTATAC	1 cut(s)	1159
Bst2UI	CCWGG	2 cut(s)	247, 1307
Bst4CI	ACNGT	4 cut(s)	63, 673, 976, 1316
Bst6I	CTCTTC	4 cut(s)	302, 891, 1161, 1210
BstAPI	GCANNNNNTGC	1 cut(s)	586
BstC8I	GCNNGC	4 cut(s)	121, 662, 690, 1265
BstDEI	CTNAG	6 cut(s)	36, 342, 452, 606, 840, 852
BstEII	GGTNACC	1 cut(s)	1301
BstF5I	GGATG	5 cut(s)	409, 475, 625, 1066, 1188
BstFNI	CGCG	2 cut(s)	750, 1267
BstKTI	GATC	2 cut(s)	187, 682
BstMAI	GTCTC	4 cut(s)	513, 893, 1168, 1200
BstMBI	GATC	2 cut(s)	184, 679
BstMCI	CGRYCG	1 cut(s)	1322
BstMWI	GCNNNNNNNGC	6 cut(s)	82, 586, 589, 724, 815, 1124
BstNI	CCWGG	2 cut(s)	247, 1307
BstNSI	RCATGY	1 cut(s)	692
BstPI	GGTNACC	1 cut(s)	1301
BstSCI	CCNGG	2 cut(s)	245, 1305
BstSFI	CTRYAG	2 cut(s)	630, 891
BstSLI	GKGCMC	2 cut(s)	102, 811
BstUI	CGCG	2 cut(s)	750, 1267
BstXI	CCANNNNNNTGG	1 cut(s)	127
BstZ17I	GTATAC	1 cut(s)	1159
Bsu36I	CCTNAGG	1 cut(s)	342
BsuRI	GGCC	3 cut(s)	5, 119, 889
BtsCI	GGATG	5 cut(s)	409, 475, 625, 1066, 1188
BtsI	GCAGTG	1 cut(s)	27
BtsIMutI	CAGTG	3 cut(s)	27, 300, 486
BveI	ACCTGC	1 cut(s)	693
Cac8I	GCNNGC	4 cut(s)	121, 662, 690, 1265
Cfr10I	RCCGGY	1 cut(s)	1103
Cfr13I	GGNCC	2 cut(s)	551, 887
Csp6I	GTAC	4 cut(s)	49, 64, 674, 1312
CspAI	ACCGGT	1 cut(s)	1103
CviAII	CATG	2 cut(s)	182, 689
CviQI	GTAC	4 cut(s)	49, 64, 674, 1312
DdeI	CTNAG	6 cut(s)	36, 342, 452, 606, 840, 852
DpnI	GATC	2 cut(s)	186, 681
DpnII	GATC	2 cut(s)	184, 679
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	4 cut(s)	302, 891, 1161, 1210
EarI	CTCTTC	4 cut(s)	302, 891, 1161, 1210
Eco130I	CCWWGG	1 cut(s)	237
Eco24I	GRGCYC	1 cut(s)	720
Eco31I	GGTCTC	1 cut(s)	1168
Eco47I	GGWCC	1 cut(s)	551
Eco57I	CTGAAG	4 cut(s)	62, 804, 1008, 1053
Eco81I	CCTNAGG	1 cut(s)	342
Eco88I	CYCGRG	2 cut(s)	1197, 1372
Eco91I	GGTNACC	1 cut(s)	1301
EcoO109I	RGGNCCY	1 cut(s)	887
EcoO65I	GGTNACC	1 cut(s)	1301
EcoRI	GAATTC	1 cut(s)	145
EcoRII	CCWGG	2 cut(s)	245, 1305
EcoT14I	CCWWGG	1 cut(s)	237
EcoT22I	ATGCAT	1 cut(s)	694
EcoT38I	GRGCYC	1 cut(s)	720
ErhI	CCWWGG	1 cut(s)	237
Esp3I	CGTCTC	1 cut(s)	1200
FaeI	CATG	2 cut(s)	185, 692
FalI	AAGNNNNNCTT	2 cut(s)	267, 299
FaqI	GGGAC	1 cut(s)	58
FatI	CATG	2 cut(s)	181, 688
FauI	CCCGC	1 cut(s)	1260
FblI	GTMKAC	2 cut(s)	1158, 1318
FokI	GGATG	5 cut(s)	396, 462, 632, 1073, 1175
FriOI	GRGCYC	1 cut(s)	720
HaeIII	GGCC	3 cut(s)	5, 119, 889
HapII	CCGG	2 cut(s)	89, 1104
Hin1II	CATG	2 cut(s)	185, 692
HincII	GTYRAC	1 cut(s)	1319
HindII	GTYRAC	1 cut(s)	1319
HinfI	GANTC	4 cut(s)	242, 400, 704, 829
HpaII	CCGG	2 cut(s)	89, 1104
HphI	GGTGA	4 cut(s)	512, 674, 858, 1313
Hpy166II	GTNNAC	6 cut(s)	100, 650, 809, 1159, 1319, 1416
Hpy188I	TCNGA	7 cut(s)	115, 207, 513, 823, 988, 1140, 1411
Hpy188III	TCNNGA	5 cut(s)	150, 344, 440, 1197, 1389
Hpy8I	GTNNAC	6 cut(s)	100, 650, 809, 1159, 1319, 1416
Hpy99I	CGWCG	1 cut(s)	167
HpyAV	CCTTC	7 cut(s)	269, 783, 878, 926, 1028, 1203, 1215
HpyCH4III	ACNGT	4 cut(s)	63, 673, 976, 1316
HpyCH4IV	ACGT	3 cut(s)	47, 326, 562
HpyCH4V	TGCA	8 cut(s)	100, 172, 338, 589, 688, 692, 809, 1111
HpyF10VI	GCNNNNNNNGC	6 cut(s)	82, 586, 589, 724, 815, 1124
HpyF3I	CTNAG	6 cut(s)	36, 342, 452, 606, 840, 852
HpySE526I	ACGT	3 cut(s)	47, 326, 562
Hsp92II	CATG	2 cut(s)	185, 692
Kzo9I	GATC	2 cut(s)	184, 679
LguI	GCTCTTC	1 cut(s)	1161
LmnI	GCTCC	1 cut(s)	535
LweI	GCATC	3 cut(s)	433, 484, 701
MaeII	ACGT	3 cut(s)	47, 326, 562
MaeIII	GTNAC	5 cut(s)	25, 55, 195, 778, 1301
MalI	GATC	2 cut(s)	186, 681
MbiI	CCGCTC	1 cut(s)	1171
MboI	GATC	2 cut(s)	184, 679
MboII	GAAGA	8 cut(s)	319, 515, 908, 938, 1106, 1178, 1218, 1227
MhlI	GDGCHC	3 cut(s)	102, 720, 811
MlsI	TGGCCA	1 cut(s)	5
MluI	ACGCGT	1 cut(s)	748
MluNI	TGGCCA	1 cut(s)	5
MmeI	TCCRAC	1 cut(s)	597
Mox20I	TGGCCA	1 cut(s)	5
Mph1103I	ATGCAT	1 cut(s)	694
MroXI	GAANNNNTTC	3 cut(s)	296, 399, 507
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	2 cut(s)	201, 1423
MslI	CAYNNNNRTG	1 cut(s)	687
Msp20I	TGGCCA	1 cut(s)	5
MspI	CCGG	2 cut(s)	89, 1104
MspR9I	CCNGG	2 cut(s)	247, 1307
Mva1269I	GAATGC	1 cut(s)	1120
MvaI	CCWGG	2 cut(s)	247, 1307
MvnI	CGCG	2 cut(s)	750, 1267
MwoI	GCNNNNNNNGC	6 cut(s)	82, 586, 589, 724, 815, 1124
NdeII	GATC	2 cut(s)	184, 679
NlaIII	CATG	2 cut(s)	185, 692
NlaIV	GGNNCC	3 cut(s)	396, 531, 1245
NmeAIII	GCCGAG	1 cut(s)	601
NmuCI	GTSAC	4 cut(s)	25, 55, 195, 1301
NsiI	ATGCAT	1 cut(s)	694
NspI	RCATGY	1 cut(s)	692
PaeI	GCATGC	1 cut(s)	692
PaeR7I	CTCGAG	1 cut(s)	1197
PaqCI	CACCTGC	1 cut(s)	693
PciSI	GCTCTTC	1 cut(s)	1161
PctI	GAATGC	1 cut(s)	1120
PdmI	GAANNNNTTC	3 cut(s)	296, 399, 507
PfeI	GAWTC	4 cut(s)	242, 400, 704, 829
PfoI	TCCNGGA	1 cut(s)	245
PinAI	ACCGGT	1 cut(s)	1103
Psp1406I	AACGTT	2 cut(s)	326, 562
Psp6I	CCWGG	2 cut(s)	245, 1305
PspEI	GGTNACC	1 cut(s)	1301
PspGI	CCWGG	2 cut(s)	245, 1305
PspN4I	GGNNCC	3 cut(s)	396, 531, 1245
PspPI	GGNCC	2 cut(s)	551, 887
RsaI	GTAC	4 cut(s)	50, 65, 675, 1313
RsaNI	GTAC	4 cut(s)	49, 64, 674, 1312
RseI	CAYNNNNRTG	1 cut(s)	687
SalI	GTCGAC	1 cut(s)	1317
SapI	GCTCTTC	1 cut(s)	1161
SaqAI	TTAA	2 cut(s)	201, 1423
Sau3AI	GATC	2 cut(s)	184, 679
Sau96I	GGNCC	2 cut(s)	551, 887
ScaI	AGTACT	1 cut(s)	65
ScrFI	CCNGG	2 cut(s)	247, 1307
SduI	GDGCHC	3 cut(s)	102, 720, 811
SfaNI	GCATC	3 cut(s)	433, 484, 701
SfcI	CTRYAG	2 cut(s)	630, 891
Sfr274I	CTCGAG	1 cut(s)	1197
SinI	GGWCC	1 cut(s)	551
SlaI	CTCGAG	1 cut(s)	1197
SmiMI	CAYNNNNRTG	1 cut(s)	687
SmlI	CTYRAG	2 cut(s)	1022, 1197
SmoI	CTYRAG	2 cut(s)	1022, 1197
SphI	GCATGC	1 cut(s)	692
SsiI	CCGC	5 cut(s)	159, 473, 1171, 1267, 1395
StyD4I	CCNGG	2 cut(s)	245, 1305
StyI	CCWWGG	1 cut(s)	237
TaaI	ACNGT	4 cut(s)	63, 673, 976, 1316
TaiI	ACGT	3 cut(s)	50, 329, 565
TaqI	TCGA	4 cut(s)	441, 1198, 1318, 1354
TaqII	GACCGA	2 cut(s)	1332, 1336
TatI	WGTACW	2 cut(s)	63, 1311
TfiI	GAWTC	4 cut(s)	242, 400, 704, 829
Tru1I	TTAA	2 cut(s)	201, 1423
Tru9I	TTAA	2 cut(s)	201, 1423
TscAI	CASTG	3 cut(s)	34, 307, 486
TseFI	GTSAC	4 cut(s)	25, 55, 195, 1301
Tsp45I	GTSAC	4 cut(s)	25, 55, 195, 1301
TspDTI	ATGAA	6 cut(s)	305, 392, 783, 924, 945, 1395
TspRI	CASTG	3 cut(s)	34, 307, 486
VneI	GTGCAC	2 cut(s)	98, 807
VpaK11BI	GGWCC	1 cut(s)	551
XapI	RAATTY	8 cut(s)	145, 232, 314, 507, 1001, 1009, 1088, 1402
XceI	RCATGY	1 cut(s)	692
XhoI	CTCGAG	1 cut(s)	1197
XmiI	GTMKAC	2 cut(s)	1158, 1318
XmnI	GAANNNNTTC	3 cut(s)	296, 399, 507
ZrmI	AGTACT	1 cut(s)	65
Zsp2I	ATGCAT	1 cut(s)	694

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.