Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0009677

Physical Location & Seq

Genomic Coordinates Forward (+)

22991905 .. 22996598

Gene Structure 4694 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1226700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1425 bp

ATGGCCACCCCCTATGCATTTCCCGTCACAGCTGCTCAGGTGGGGACGTACTTTGTGACGCAGTACTACCAAGTGCTTCAGCAGCAGCCGGACTTTGTGCACCAGTTCTACTCCGAGGCCAGCACAATGGTTCGTGCCGACGGCAATTCCAGAGCCACCGCAGTTGCAATGCAGCAAATCCATGATCTTGTTATGTCACTCAATCTGACTGGATTCGAAATCAACAAAGCAAATTCCTTGGAATCTTGGAATGGAGGTGTTCTTGTTCTGGTTGAAGGCTACCTTGAAATGAAAACTTTCACTGGGAGGAGGAAATTTGCGCAAACATTTTTCCTTGCACCTCAGGAGAGAGGTTATTTTGTGCTGAACGATATATTTCACTTCGTTGATGAGGAACCGATTCATCATCATCCAGCAGTCTTATTAGCCCAAAACAATCTTGACGCCAAGCTAAGTGCTCCTGCTACAATTTCGCAGCCAGTGGCTAACTATTATGTGGGTGGAGAGATTCAGACAAGAGACTTTGTCGCTCCTGCTGTCAAAGAAAATGGTCCAGTTGAAAGTTATGGCTTTGCAGAGCAACAGTTGCAGCAAGTTGTTGAAACTGAGAACTTGTTGGATGATGGTGCTGTAGAGCAATCAAATGGTGCACTTCAAAGCATGCCGAATACCATACAAGATCACCTGCATGCATCTGTTGAGGAATCTATTGGAGAGCCTCAAAAGCAGACCTATGCTTCTATATTACGCGTTGCCAAGGGGCAACCTGTGCCTTCTGTAGCTCCCCAGCGTTCATCTAATAAGAGTGCACCAATGGCTTCAGACTGGAATCACTATCCTCAGTCCACTGCTCATCAATCCATTTCATCATCTAATACATTTGAAAGGCCAGTAGCAGAGACAGCAGATGAGGTTGCCGCCCTAGAAGATGAAGGTGAAATAAAGTCTGTTTATGTAAGAAACTTGCCGACTACTGTGTCCCCATCTGAAGTGGAGGAGGAATTTGTCAATTTTGGAAAACTCAAGCAGCCTGAAGGAGTGGTCATTAGGAGTCGCAAGGATGTTGGTGTTTGCTATGCGTTTGTTGAATTTGAAGATATTACCGGTGTTCAGAATGCCGTCAAGGCTGGTAGTGTTCAAATTGCTGGACGACAGGTATACATTGAAGAACGGAGACCAAACAGCAAGATCCCGTCTCGAGTGGGAAGAAGGGGAAGAGGAAGTGGCAGCTACCAAGCAGAGGCACCGAGGGGTCGTTTTGGTTCTCGGAGTTTTGGGAGGGGAGGTGGCTATGACGGAGGTGACCAGGACTACAGCCGACCAAGGGGAAATGGCTACTATAGGCCAAGCCCTCGACAAGACAGAGGGAACTCGGGTTATCAATCATCAAGAAGCGGACAAAATTCATCGGAGTTCACTAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006508 GO:0006807 GO:0006914 GO:0008150 GO:0008152 GO:0009056 GO:0009987 GO:0010494 GO:0016236 GO:0016579 GO:0019538 GO:0032386 GO:0032879 GO:0032991 GO:0034517 GO:0035770 GO:0035973 GO:0036211 GO:0036464 GO:0043021 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043412 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044877 GO:0050789 GO:0050794 GO:0051049 GO:0060341 GO:0060627 GO:0060628 GO:0061912 GO:0061919 GO:0065007 GO:0070646 GO:0070647 GO:0071704 GO:0097159 GO:1901363 GO:1901564 GO:1905538 GO:1990861 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NTF2 RRM_1

Protein Analysis

475

Amino Acids

52.22

Weight (kDa)

5.5

Isoelectric Point (pI)

59.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NTF2	PF02136	14 - 128	2.5e-25	Nuclear transport factor 2 (NTF2) domain
RRM_1	PF00076	317 - 382	5.8e-07	RNA recognition motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009677)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g04660 FvH4_4g04660
malus_domestica	MD13G1221800.v1.1 MD16G1226700.v1.1
prunus_persica	Prupe.1G048900_v2.0.a1 Prupe.1G048900_v2.0.a1
pyrus_communis	pycom13g19610 pycom16g18980
rosa_chinensis	RchiOBHm_Chr4g0395471
rosa_laevigata	RLG00000009620
rosa_multiflora	Rmu_sc0001951.1_g000003 Rmu_sc0011701.1_g000032
rosa_roxburghii	Rroxscaffold_5G00340410
rosa_rugosa	Rorug03G0356000 Rorug03G0356000 Rorug03G0356000
rosa_samantha	Rh4AG060400 Rh4AG062100 Rh4BG059300 Rh4CG065000 Rh4DG055300
rosa_wichuraiana	Rw4G005070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	693
AasI	GACNNNNNNGTC	1 cut(s)	976
Acc16I	TGCGCA	1 cut(s)	321
Acc36I	ACCTGC	1 cut(s)	693
AccB1I	GGYRCC	1 cut(s)	1243
AccI	GTMKAC	1 cut(s)	1158
AccII	CGCG	1 cut(s)	750
AciI	CCGC	3 cut(s)	159, 918, 1395
AclWI	GGATC	1 cut(s)	1183
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	5 cut(s)	232, 314, 1001, 1088, 1402
AcuI	CTGAAG	4 cut(s)	62, 804, 1008, 1053
AcyI	GRCGYC	1 cut(s)	444
AfaI	GTAC	2 cut(s)	50, 65
AfiI	CCNNNNNNNGG	2 cut(s)	1240, 1324
AflIII	ACRYGT	1 cut(s)	748
AgeI	ACCGGT	1 cut(s)	1103
AjnI	CCWGG	1 cut(s)	1305
AluBI	AGCT	4 cut(s)	32, 451, 782, 1230
AluI	AGCT	4 cut(s)	32, 451, 782, 1230
Alw21I	GWGCWC	4 cut(s)	102, 460, 652, 811
Alw26I	GTCTC	4 cut(s)	513, 893, 1168, 1200
Alw44I	GTGCAC	3 cut(s)	98, 648, 807
AlwI	GGATC	1 cut(s)	1183
Ama87I	CYCGRG	2 cut(s)	1197, 1372
AoxI	GGCC	4 cut(s)	3, 117, 887, 1343
ApaLI	GTGCAC	3 cut(s)	98, 648, 807
ApeKI	GCWGC	8 cut(s)	32, 82, 85, 172, 475, 589, 1027, 1227
ApoI	RAATTY	5 cut(s)	232, 314, 1001, 1088, 1402
AsiGI	ACCGGT	1 cut(s)	1103
Asp700I	GAANNNNTTC	2 cut(s)	296, 399
AspLEI	GCGC	1 cut(s)	322
AspS9I	GGNCC	1 cut(s)	551
AsuHPI	GGTGA	3 cut(s)	674, 947, 1313
AsuII	TTCGAA	1 cut(s)	216
AvaI	CYCGRG	2 cut(s)	1197, 1372
AvaII	GGWCC	1 cut(s)	551
AxyI	CCTNAGG	1 cut(s)	342
BaeGI	GKGCMC	3 cut(s)	102, 652, 811
BalI	TGGCCA	1 cut(s)	5
BanI	GGYRCC	1 cut(s)	1243
Bbv12I	GWGCWC	4 cut(s)	102, 460, 652, 811
BbvI	GCAGC	8 cut(s)	19, 94, 97, 184, 487, 601, 1039, 1239
BccI	CCATC	2 cut(s)	617, 991
BceAI	ACGGC	2 cut(s)	157, 1103
BciT130I	CCWGG	1 cut(s)	1307
BcoDI	GTCTC	4 cut(s)	513, 893, 1168, 1200
BfaI	CTAG	1 cut(s)	923
BfmI	CTRYAG	4 cut(s)	630, 777, 1312, 1339
BfuAI	ACCTGC	1 cut(s)	693
BglI	GCCNNNNNGGC	1 cut(s)	1124
BisI	GCNGC	9 cut(s)	33, 83, 86, 173, 476, 590, 918, 1028, 1228
BlsI	GCNGC	9 cut(s)	34, 84, 87, 174, 477, 591, 919, 1029, 1229
BmcAI	AGTACT	1 cut(s)	65
Bme1390I	CCNGG	1 cut(s)	1307
Bme18I	GGWCC	1 cut(s)	551
BmeT110I	CYCGRG	2 cut(s)	1197, 1372
BmgT120I	GGNCC	1 cut(s)	551
BmiI	GGNNCC	2 cut(s)	396, 1245
BmrFI	CCNGG	1 cut(s)	1307
BmrI	ACTGGG	1 cut(s)	312
BmsI	GCATC	1 cut(s)	701
BmuI	ACTGGG	1 cut(s)	312
Bpu10I	CCTNAGC	1 cut(s)	36
Bpu14I	TTCGAA	1 cut(s)	216
BpuEI	CTTGAG	1 cut(s)	1007
BsaHI	GRCGYC	1 cut(s)	444
BsaI	GGTCTC	1 cut(s)	1168
BsaJI	CCNNGG	5 cut(s)	114, 237, 756, 1247, 1322
BsaWI	WCCGGW	1 cut(s)	1103
BsaXI	ACNNNNNCTCC	2 cut(s)	1270, 1300
Bsc4I	CCNNNNNNNGG	2 cut(s)	1240, 1324
Bse118I	RCCGGY	1 cut(s)	1103
Bse1I	ACTGG	7 cut(s)	103, 214, 307, 479, 554, 830, 890
Bse21I	CCTNAGG	1 cut(s)	342
Bse3DI	GCAATG	1 cut(s)	174
BseBI	CCWGG	1 cut(s)	1307
BseDI	CCNNGG	5 cut(s)	114, 237, 756, 1247, 1322
BseGI	GGATG	3 cut(s)	409, 625, 1066
BseLI	CCNNNNNNNGG	2 cut(s)	1240, 1324
BseMI	GCAATG	1 cut(s)	174
BseMII	CTCAG	4 cut(s)	50, 356, 597, 854
BseNI	ACTGG	7 cut(s)	103, 214, 307, 479, 554, 830, 890
BseRI	GAGGAG	2 cut(s)	322, 1010
BseSI	GKGCMC	3 cut(s)	102, 652, 811
BseXI	GCAGC	8 cut(s)	19, 94, 97, 184, 487, 601, 1039, 1239
BseYI	CCCAGC	1 cut(s)	786
Bsh1236I	CGCG	1 cut(s)	750
BshFI	GGCC	4 cut(s)	5, 119, 889, 1345
BshNI	GGYRCC	1 cut(s)	1243
BshTI	ACCGGT	1 cut(s)	1103
BsiHKAI	GWGCWC	4 cut(s)	102, 460, 652, 811
BsiHKCI	CYCGRG	2 cut(s)	1197, 1372
BsiSI	CCGG	2 cut(s)	89, 1104
BslFI	GGGAC	2 cut(s)	58, 964
BslI	CCNNNNNNNGG	2 cut(s)	1240, 1324
BsmAI	GTCTC	4 cut(s)	513, 893, 1168, 1200
BsmBI	CGTCTC	1 cut(s)	1200
BsmFI	GGGAC	2 cut(s)	58, 964
BsmI	GAATGC	1 cut(s)	1120
BsnI	GGCC	4 cut(s)	5, 119, 889, 1345
Bso31I	GGTCTC	1 cut(s)	1168
BsoBI	CYCGRG	2 cut(s)	1197, 1372
Bsp119I	TTCGAA	1 cut(s)	216
Bsp1286I	GDGCHC	4 cut(s)	102, 460, 652, 811
Bsp143I	GATC	3 cut(s)	184, 679, 1188
BspACI	CCGC	3 cut(s)	159, 918, 1395
BspANI	GGCC	4 cut(s)	5, 119, 889, 1345
BspCNI	CTCAG	4 cut(s)	49, 355, 598, 853
BspFNI	CGCG	1 cut(s)	750
BspLI	GGNNCC	2 cut(s)	396, 1245
BspMI	ACCTGC	1 cut(s)	693
BspPI	GGATC	1 cut(s)	1183
BspT104I	TTCGAA	1 cut(s)	216
BspT107I	GGYRCC	1 cut(s)	1243
BspTNI	GGTCTC	1 cut(s)	1168
BsrDI	GCAATG	1 cut(s)	174
BsrFI	RCCGGY	1 cut(s)	1103
BsrI	ACTGG	7 cut(s)	103, 214, 307, 479, 554, 830, 890
BssAI	RCCGGY	1 cut(s)	1103
BssECI	CCNNGG	5 cut(s)	114, 237, 756, 1247, 1322
BssMI	GATC	3 cut(s)	184, 679, 1188
BssNAI	GTATAC	1 cut(s)	1159
BssNI	GRCGYC	1 cut(s)	444
BssT1I	CCWWGG	3 cut(s)	237, 756, 1322
Bst1107I	GTATAC	1 cut(s)	1159
Bst2UI	CCWGG	1 cut(s)	1307
Bst4CI	ACNGT	2 cut(s)	585, 976
Bst6I	CTCTTC	1 cut(s)	1210
BstACI	GRCGYC	1 cut(s)	444
BstAPI	GCANNNNNTGC	2 cut(s)	586, 769
BstBI	TTCGAA	1 cut(s)	216
BstC8I	GCNNGC	3 cut(s)	121, 662, 690
BstDEI	CTNAG	5 cut(s)	36, 342, 452, 606, 840
BstEII	GGTNACC	1 cut(s)	1301
BstF5I	GGATG	3 cut(s)	409, 625, 1066
BstFNI	CGCG	1 cut(s)	750
BstHHI	GCGC	1 cut(s)	322
BstKTI	GATC	3 cut(s)	187, 682, 1191
BstMAI	GTCTC	4 cut(s)	513, 893, 1168, 1200
BstMBI	GATC	3 cut(s)	184, 679, 1188
BstMWI	GCNNNNNNNGC	7 cut(s)	82, 586, 724, 769, 815, 902, 1124
BstNI	CCWGG	1 cut(s)	1307
BstNSI	RCATGY	2 cut(s)	664, 692
BstPI	GGTNACC	1 cut(s)	1301
BstSCI	CCNGG	1 cut(s)	1305
BstSFI	CTRYAG	4 cut(s)	630, 777, 1312, 1339
BstSLI	GKGCMC	3 cut(s)	102, 652, 811
BstUI	CGCG	1 cut(s)	750
BstV1I	GCAGC	8 cut(s)	19, 94, 97, 184, 487, 601, 1039, 1239
BstX2I	RGATCY	1 cut(s)	1188
BstXI	CCANNNNNNTGG	1 cut(s)	127
BstYI	RGATCY	1 cut(s)	1188
BstZ17I	GTATAC	1 cut(s)	1159
Bsu36I	CCTNAGG	1 cut(s)	342
BsuRI	GGCC	4 cut(s)	5, 119, 889, 1345
BtsCI	GGATG	3 cut(s)	409, 625, 1066
BtsI	GCAGTG	1 cut(s)	846
BtsIMutI	CAGTG	3 cut(s)	300, 486, 846
BveI	ACCTGC	1 cut(s)	693
Cac8I	GCNNGC	3 cut(s)	121, 662, 690
CfoI	GCGC	1 cut(s)	322
Cfr10I	RCCGGY	1 cut(s)	1103
Cfr13I	GGNCC	1 cut(s)	551
CseI	GACGC	2 cut(s)	67, 452
Csp6I	GTAC	2 cut(s)	49, 64
CspAI	ACCGGT	1 cut(s)	1103
CspCI	CAANNNNNGTGG	2 cut(s)	145, 180
CviAII	CATG	3 cut(s)	182, 661, 689
CviQI	GTAC	2 cut(s)	49, 64
DdeI	CTNAG	5 cut(s)	36, 342, 452, 606, 840
DpnI	GATC	3 cut(s)	186, 681, 1190
DpnII	GATC	3 cut(s)	184, 679, 1188
DrdI	GACNNNNNNGTC	1 cut(s)	976
DseDI	GACNNNNNNGTC	1 cut(s)	976
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	1 cut(s)	1210
EarI	CTCTTC	1 cut(s)	1210
Eco130I	CCWWGG	3 cut(s)	237, 756, 1322
Eco31I	GGTCTC	1 cut(s)	1168
Eco47I	GGWCC	1 cut(s)	551
Eco57I	CTGAAG	4 cut(s)	62, 804, 1008, 1053
Eco81I	CCTNAGG	1 cut(s)	342
Eco88I	CYCGRG	2 cut(s)	1197, 1372
Eco91I	GGTNACC	1 cut(s)	1301
EcoO65I	GGTNACC	1 cut(s)	1301
EcoRII	CCWGG	1 cut(s)	1305
EcoT14I	CCWWGG	3 cut(s)	237, 756, 1322
EcoT22I	ATGCAT	2 cut(s)	19, 694
ErhI	CCWWGG	3 cut(s)	237, 756, 1322
Esp3I	CGTCTC	1 cut(s)	1200
FaeI	CATG	3 cut(s)	185, 664, 692
FalI	AAGNNNNNCTT	2 cut(s)	267, 299
FaqI	GGGAC	2 cut(s)	58, 964
FatI	CATG	3 cut(s)	181, 660, 688
FblI	GTMKAC	1 cut(s)	1158
Fnu4HI	GCNGC	9 cut(s)	33, 83, 86, 173, 476, 590, 918, 1028, 1228
FokI	GGATG	3 cut(s)	396, 632, 1073
Fsp4HI	GCNGC	9 cut(s)	33, 83, 86, 173, 476, 590, 918, 1028, 1228
FspBI	CTAG	1 cut(s)	923
FspI	TGCGCA	1 cut(s)	321
GlaI	GCGC	1 cut(s)	321
GluI	GCNGC	9 cut(s)	33, 83, 86, 173, 476, 590, 918, 1028, 1228
GsaI	CCCAGC	1 cut(s)	790
HaeIII	GGCC	4 cut(s)	5, 119, 889, 1345
HapII	CCGG	2 cut(s)	89, 1104
HgaI	GACGC	2 cut(s)	67, 452
HhaI	GCGC	1 cut(s)	322
Hin1I	GRCGYC	1 cut(s)	444
Hin1II	CATG	3 cut(s)	185, 664, 692
Hin6I	GCGC	1 cut(s)	320
HinP1I	GCGC	1 cut(s)	320
HinfI	GANTC	7 cut(s)	213, 242, 400, 508, 704, 829, 1051
HpaII	CCGG	2 cut(s)	89, 1104
HphI	GGTGA	3 cut(s)	674, 947, 1313
Hpy166II	GTNNAC	6 cut(s)	100, 650, 809, 846, 1159, 1416
Hpy188I	TCNGA	8 cut(s)	115, 207, 513, 823, 988, 1113, 1269, 1411
Hpy188III	TCNNGA	5 cut(s)	150, 344, 440, 1197, 1389
Hpy8I	GTNNAC	6 cut(s)	100, 650, 809, 846, 1159, 1416
Hpy99I	CGWCG	1 cut(s)	143
HpyAV	CCTTC	5 cut(s)	269, 783, 926, 1028, 1203
HpyCH4III	ACNGT	2 cut(s)	585, 976
HpyCH4IV	ACGT	1 cut(s)	47
HpyF10VI	GCNNNNNNNGC	7 cut(s)	82, 586, 724, 769, 815, 902, 1124
HpyF3I	CTNAG	5 cut(s)	36, 342, 452, 606, 840
HpySE526I	ACGT	1 cut(s)	47
Hsp92I	GRCGYC	1 cut(s)	444
Hsp92II	CATG	3 cut(s)	185, 664, 692
HspAI	GCGC	1 cut(s)	320
Kzo9I	GATC	3 cut(s)	184, 679, 1188
LmnI	GCTCC	3 cut(s)	463, 535, 787
Lsp1109I	GCAGC	8 cut(s)	19, 94, 97, 184, 487, 601, 1039, 1239
LweI	GCATC	1 cut(s)	701
MaeI	CTAG	1 cut(s)	923
MaeII	ACGT	1 cut(s)	47
MaeIII	GTNAC	4 cut(s)	25, 55, 195, 1301
MalI	GATC	3 cut(s)	186, 681, 1190
MboI	GATC	3 cut(s)	184, 679, 1188
MboII	GAAGA	5 cut(s)	938, 1106, 1178, 1218, 1227
MflI	RGATCY	1 cut(s)	1188
MhlI	GDGCHC	4 cut(s)	102, 460, 652, 811
MlsI	TGGCCA	1 cut(s)	5
MluI	ACGCGT	1 cut(s)	748
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	1 cut(s)	1060
MmeI	TCCRAC	1 cut(s)	597
Mox20I	TGGCCA	1 cut(s)	5
Mph1103I	ATGCAT	2 cut(s)	19, 694
MroXI	GAANNNNTTC	2 cut(s)	296, 399
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	1 cut(s)	1423
MslI	CAYNNNNRTG	1 cut(s)	687
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	1 cut(s)	32
MspI	CCGG	2 cut(s)	89, 1104
MspR9I	CCNGG	1 cut(s)	1307
Mva1269I	GAATGC	1 cut(s)	1120
MvaI	CCWGG	1 cut(s)	1307
MvnI	CGCG	1 cut(s)	750
MwoI	GCNNNNNNNGC	7 cut(s)	82, 586, 724, 769, 815, 902, 1124
NdeII	GATC	3 cut(s)	184, 679, 1188
NlaIII	CATG	3 cut(s)	185, 664, 692
NlaIV	GGNNCC	2 cut(s)	396, 1245
NmuCI	GTSAC	4 cut(s)	25, 55, 195, 1301
NsbI	TGCGCA	1 cut(s)	321
NsiI	ATGCAT	2 cut(s)	19, 694
NspI	RCATGY	2 cut(s)	664, 692
NspV	TTCGAA	1 cut(s)	216
PaeI	GCATGC	2 cut(s)	664, 692
PaeR7I	CTCGAG	1 cut(s)	1197
PaqCI	CACCTGC	1 cut(s)	693
PctI	GAATGC	1 cut(s)	1120
PdmI	GAANNNNTTC	2 cut(s)	296, 399
PfeI	GAWTC	6 cut(s)	213, 242, 400, 508, 704, 829
PflFI	GACNNNGTC	1 cut(s)	524
PinAI	ACCGGT	1 cut(s)	1103
PkrI	GCNGC	9 cut(s)	34, 84, 87, 174, 477, 591, 919, 1029, 1229
PleI	GAGTC	1 cut(s)	1059
PpsI	GAGTC	1 cut(s)	1059
Psp6I	CCWGG	1 cut(s)	1305
PspEI	GGTNACC	1 cut(s)	1301
PspFI	CCCAGC	1 cut(s)	786
PspGI	CCWGG	1 cut(s)	1305
PspN4I	GGNNCC	2 cut(s)	396, 1245
PspPI	GGNCC	1 cut(s)	551
PsuI	RGATCY	1 cut(s)	1188
PsyI	GACNNNGTC	1 cut(s)	524
PvuII	CAGCTG	1 cut(s)	32
RsaI	GTAC	2 cut(s)	50, 65
RsaNI	GTAC	2 cut(s)	49, 64
RseI	CAYNNNNRTG	1 cut(s)	687
SaqAI	TTAA	1 cut(s)	1423
SatI	GCNGC	9 cut(s)	33, 83, 86, 173, 476, 590, 918, 1028, 1228
Sau3AI	GATC	3 cut(s)	184, 679, 1188
Sau96I	GGNCC	1 cut(s)	551
ScaI	AGTACT	1 cut(s)	65
SchI	GAGTC	1 cut(s)	1060
ScrFI	CCNGG	1 cut(s)	1307
SduI	GDGCHC	4 cut(s)	102, 460, 652, 811
SfaNI	GCATC	1 cut(s)	701
SfcI	CTRYAG	4 cut(s)	630, 777, 1312, 1339
Sfr274I	CTCGAG	1 cut(s)	1197
SfuI	TTCGAA	1 cut(s)	216
SinI	GGWCC	1 cut(s)	551
SlaI	CTCGAG	1 cut(s)	1197
SmiMI	CAYNNNNRTG	1 cut(s)	687
SmlI	CTYRAG	2 cut(s)	1022, 1197
SmoI	CTYRAG	2 cut(s)	1022, 1197
SphI	GCATGC	2 cut(s)	664, 692
SsiI	CCGC	3 cut(s)	159, 918, 1395
SspMI	CTAG	1 cut(s)	923
StyD4I	CCNGG	1 cut(s)	1305
StyI	CCWWGG	3 cut(s)	237, 756, 1322
TaaI	ACNGT	2 cut(s)	585, 976
TaiI	ACGT	1 cut(s)	50
TaqI	TCGA	3 cut(s)	216, 1198, 1354
TatI	WGTACW	1 cut(s)	63
TauI	GCSGC	1 cut(s)	920
TfiI	GAWTC	6 cut(s)	213, 242, 400, 508, 704, 829
Tru1I	TTAA	1 cut(s)	1423
Tru9I	TTAA	1 cut(s)	1423
TscAI	CASTG	3 cut(s)	307, 486, 853
TseFI	GTSAC	4 cut(s)	25, 55, 195, 1301
TseI	GCWGC	8 cut(s)	32, 82, 85, 172, 475, 589, 1027, 1227
Tsp45I	GTSAC	4 cut(s)	25, 55, 195, 1301
TspDTI	ATGAA	6 cut(s)	305, 392, 783, 855, 945, 1395
TspGWI	ACGGA	2 cut(s)	1186, 1311
TspRI	CASTG	3 cut(s)	307, 486, 853
Tth111I	GACNNNGTC	1 cut(s)	524
VneI	GTGCAC	3 cut(s)	98, 648, 807
VpaK11BI	GGWCC	1 cut(s)	551
XapI	RAATTY	5 cut(s)	232, 314, 1001, 1088, 1402
XceI	RCATGY	2 cut(s)	664, 692
XhoI	CTCGAG	1 cut(s)	1197
XmiI	GTMKAC	1 cut(s)	1158
XmnI	GAANNNNTTC	2 cut(s)	296, 399
XspI	CTAG	1 cut(s)	923
ZrmI	AGTACT	1 cut(s)	65
Zsp2I	ATGCAT	2 cut(s)	19, 694

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.