MD13G1263000.v1.1

Protein GLUTAMINE DUMPER

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0020865
Physical Location & Seq
Forward (+)
33446627 .. 33447172
546 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1263000.v1.1.491

Sequence Viewer

Length: 546 bp
ATGTCAGGGAAAGAGCCTTTTAACGTGACAGCCAGAACATCGGGGATCACGACGACACAGAGTTCACCGTGGCATTCCCCGGTGCCCTACTTGTTCGGTGGGCTGGCAGCCATGTTGGGTCTAATTGCGTTTGCGCTTCTGATCTTAGCATGCTCCTACTGGAAGCTCTCCGGATACCTCGAAAACAACGAAAACGGATCAGAGCAGGACCTGGAAGCTGGAGAAGGCGGAAAAGGCAACGAGTCCGCCCAGAAGGTCCCGAAGGTTTTCGAAGAGAAAATCTTGGTGATCATGGCCGGAGACGCCAAGCCAACTTTCTTGGCCACACCCATGTCAAGTAGGACTTCATCTTTTGGCGACAATACTAACAGCACTTGTAGCTGTAGCCAAAAGACTGATAAGTCAGTGGAAATGAGTGAGACGACTGCTGACAATAAGCAAGGAAGTAGTGATGGCAGTGATCAGCATCAGGTACAAGTCACCGATGAGAACATGGACAGTACCCATGAGGCCTCAGATCACTCAGGTCAGAGAGCCAGTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

182

Amino Acids

19.28

Weight (kDa)

4.73

Isoelectric Point (pI)

45.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020865)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G57685
malus_domestica MD13G1263000.v1.1 MD16G1264600.v1.1
prunus_persica Prupe.1G094400_v2.0.a1
pyrus_communis pycom16g23390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 400
AccB1I GGYRCC 1 cut(s) 82
AccIII TCCGGA 1 cut(s) 170
AciI CCGC 2 cut(s) 228, 246
AclWI GGATC 2 cut(s) 53, 205
AcoI YGGCCR 2 cut(s) 294, 321
AcyI GRCGYC 1 cut(s) 303
AfaI GTAC 2 cut(s) 474, 502
AjnI CCWGG 1 cut(s) 210
AjuI GAANNNNNNNTTGG 2 cut(s) 299, 331
AluBI AGCT 3 cut(s) 166, 218, 381
AluI AGCT 3 cut(s) 166, 218, 381
Alw26I GTCTC 2 cut(s) 294, 413
AlwI GGATC 2 cut(s) 53, 205
AlwNI CAGNNNCTG 1 cut(s) 211
Aor13HI TCCGGA 1 cut(s) 170
AoxI GGCC 3 cut(s) 294, 321, 510
ApeKI GCWGC 1 cut(s) 107
Asp700I GAANNNNTTC 1 cut(s) 266
AspLEI GCGC 1 cut(s) 136
AspS9I GGNCC 2 cut(s) 208, 256
AsuC2I CCSGG 1 cut(s) 80
AsuHPI GGTGA 3 cut(s) 57, 298, 472
AsuII TTCGAA 1 cut(s) 270
AvaII GGWCC 2 cut(s) 208, 256
BaeGI GKGCMC 1 cut(s) 87
BalI TGGCCA 1 cut(s) 323
BanI GGYRCC 1 cut(s) 82
BbvI GCAGC 1 cut(s) 119
BccI CCATC 1 cut(s) 446
BciT130I CCWGG 1 cut(s) 212
BciVI GTATCC 1 cut(s) 167
BclI TGATCA 2 cut(s) 288, 460
BcnI CCSGG 1 cut(s) 80
BcoDI GTCTC 2 cut(s) 294, 413
BfmI CTRYAG 1 cut(s) 382
BfuI GTATCC 1 cut(s) 167
BisI GCNGC 1 cut(s) 108
BlsI GCNGC 1 cut(s) 109
Bme1390I CCNGG 2 cut(s) 80, 212
Bme18I GGWCC 2 cut(s) 208, 256
BmgT120I GGNCC 2 cut(s) 208, 256
BmiI GGNNCC 2 cut(s) 84, 258
BmrFI CCNGG 2 cut(s) 80, 212
BmsI GCATC 1 cut(s) 475
BpmI CTGGAG 1 cut(s) 240
Bpu14I TTCGAA 1 cut(s) 270
BpuMI CCSGG 1 cut(s) 80
BsaBI GATNNNNATC 1 cut(s) 465
BsaHI GRCGYC 1 cut(s) 303
BsaJI CCNNGG 2 cut(s) 68, 78
BsaWI WCCGGW 1 cut(s) 170
Bse1I ACTGG 2 cut(s) 164, 537
Bse8I GATNNNNATC 1 cut(s) 465
BseAI TCCGGA 1 cut(s) 170
BseBI CCWGG 1 cut(s) 212
BseDI CCNNGG 2 cut(s) 68, 78
BseJI GATNNNNATC 1 cut(s) 465
BseMII CTCAG 2 cut(s) 528, 537
BseNI ACTGG 2 cut(s) 164, 537
BseSI GKGCMC 1 cut(s) 87
BseXI GCAGC 1 cut(s) 119
BshFI GGCC 3 cut(s) 296, 323, 512
BshNI GGYRCC 1 cut(s) 82
BsiSI CCGG 3 cut(s) 80, 171, 297
BslFI GGGAC 1 cut(s) 242
BsmAI GTCTC 2 cut(s) 294, 413
BsmBI CGTCTC 2 cut(s) 294, 413
BsmFI GGGAC 1 cut(s) 242
BsmI GAATGC 1 cut(s) 73
BsnI GGCC 3 cut(s) 296, 323, 512
Bsp119I TTCGAA 1 cut(s) 270
Bsp1286I GDGCHC 1 cut(s) 87
Bsp13I TCCGGA 1 cut(s) 170
Bsp143I GATC 6 cut(s) 45, 141, 197, 288, 460, 517
BspACI CCGC 2 cut(s) 228, 246
BspANI GGCC 3 cut(s) 296, 323, 512
BspCNI CTCAG 2 cut(s) 527, 536
BspEI TCCGGA 1 cut(s) 170
BspLI GGNNCC 2 cut(s) 84, 258
BspPI GGATC 2 cut(s) 53, 205
BspT104I TTCGAA 1 cut(s) 270
BspT107I GGYRCC 1 cut(s) 82
BsrI ACTGG 2 cut(s) 164, 537
BssECI CCNNGG 2 cut(s) 68, 78
BssMI GATC 6 cut(s) 45, 141, 197, 288, 460, 517
BssNI GRCGYC 1 cut(s) 303
Bst2UI CCWGG 1 cut(s) 212
Bst4CI ACNGT 2 cut(s) 69, 500
Bst6I CTCTTC 1 cut(s) 267
BstACI GRCGYC 1 cut(s) 303
BstBI TTCGAA 1 cut(s) 270
BstC8I GCNNGC 2 cut(s) 105, 151
BstDEI CTNAG 3 cut(s) 145, 514, 523
BstDSI CCRYGG 1 cut(s) 68
BstHHI GCGC 1 cut(s) 136
BstKTI GATC 6 cut(s) 48, 144, 200, 291, 463, 520
BstMAI GTCTC 2 cut(s) 294, 413
BstMBI GATC 6 cut(s) 45, 141, 197, 288, 460, 517
BstMWI GCNNNNNNNGC 3 cut(s) 234, 302, 378
BstNI CCWGG 1 cut(s) 212
BstNSI RCATGY 1 cut(s) 153
BstSCI CCNGG 2 cut(s) 78, 210
BstSFI CTRYAG 1 cut(s) 382
BstSLI GKGCMC 1 cut(s) 87
BstV1I GCAGC 1 cut(s) 119
BsuI GTATCC 1 cut(s) 167
BsuRI GGCC 3 cut(s) 296, 323, 512
BtgI CCRYGG 1 cut(s) 68
BtsI GCAGTG 1 cut(s) 463
BtsIMutI CAGTG 2 cut(s) 411, 463
Cac8I GCNNGC 2 cut(s) 105, 151
CaiI CAGNNNCTG 1 cut(s) 211
CfoI GCGC 1 cut(s) 136
Cfr13I GGNCC 2 cut(s) 208, 256
CseI GACGC 1 cut(s) 311
Csp6I GTAC 2 cut(s) 473, 501
CviAII CATG 6 cut(s) 112, 150, 292, 331, 493, 506
CviQI GTAC 2 cut(s) 473, 501
DdeI CTNAG 3 cut(s) 145, 514, 523
DpnI GATC 6 cut(s) 47, 143, 199, 290, 462, 519
DpnII GATC 6 cut(s) 45, 141, 197, 288, 460, 517
DrdI GACNNNNNNGTC 1 cut(s) 400
DseDI GACNNNNNNGTC 1 cut(s) 400
EaeI YGGCCR 2 cut(s) 294, 321
Eam1104I CTCTTC 1 cut(s) 267
EarI CTCTTC 1 cut(s) 267
EciI GGCGGA 2 cut(s) 235, 243
Eco147I AGGCCT 1 cut(s) 512
Eco47I GGWCC 2 cut(s) 208, 256
EcoO109I RGGNCCY 2 cut(s) 208, 256
EcoRII CCWGG 1 cut(s) 210
Esp3I CGTCTC 2 cut(s) 294, 413
FaeI CATG 6 cut(s) 115, 153, 295, 334, 496, 509
FaiI YATR 6 cut(s) 113, 151, 293, 332, 494, 507
FalI AAGNNNNNCTT 2 cut(s) 328, 360
FaqI GGGAC 1 cut(s) 242
FatI CATG 6 cut(s) 111, 149, 291, 330, 492, 505
FbaI TGATCA 2 cut(s) 288, 460
Fnu4HI GCNGC 1 cut(s) 108
Fsp4HI GCNGC 1 cut(s) 108
GlaI GCGC 1 cut(s) 135
GluI GCNGC 1 cut(s) 108
GsuI CTGGAG 1 cut(s) 240
HaeIII GGCC 3 cut(s) 296, 323, 512
HapII CCGG 3 cut(s) 80, 171, 297
HgaI GACGC 1 cut(s) 311
HhaI GCGC 1 cut(s) 136
Hin1I GRCGYC 1 cut(s) 303
Hin1II CATG 6 cut(s) 115, 153, 295, 334, 496, 509
Hin6I GCGC 1 cut(s) 134
HinP1I GCGC 1 cut(s) 134
HinfI GANTC 1 cut(s) 242
HpaII CCGG 3 cut(s) 80, 171, 297
HphI GGTGA 3 cut(s) 57, 298, 472
Hpy166II GTNNAC 1 cut(s) 65
Hpy188I TCNGA 4 cut(s) 141, 202, 517, 531
Hpy188III TCNNGA 3 cut(s) 49, 171, 259
Hpy8I GTNNAC 1 cut(s) 65
Hpy99I CGWCG 1 cut(s) 55
HpyAV CCTTC 3 cut(s) 218, 247, 256
HpyCH4III ACNGT 2 cut(s) 69, 500
HpyCH4IV ACGT 1 cut(s) 24
HpyF10VI GCNNNNNNNGC 3 cut(s) 234, 302, 378
HpyF3I CTNAG 3 cut(s) 145, 514, 523
HpySE526I ACGT 1 cut(s) 24
Hsp92I GRCGYC 1 cut(s) 303
Hsp92II CATG 6 cut(s) 115, 153, 295, 334, 496, 509
HspAI GCGC 1 cut(s) 134
Kpn2I TCCGGA 1 cut(s) 170
Ksp22I TGATCA 2 cut(s) 288, 460
Kzo9I GATC 6 cut(s) 45, 141, 197, 288, 460, 517
LmnI GCTCC 1 cut(s) 158
Lsp1109I GCAGC 1 cut(s) 119
LweI GCATC 1 cut(s) 475
MaeII ACGT 1 cut(s) 24
MaeIII GTNAC 2 cut(s) 25, 478
MalI GATC 6 cut(s) 47, 143, 199, 290, 462, 519
MboI GATC 6 cut(s) 45, 141, 197, 288, 460, 517
MboII GAAGA 1 cut(s) 284
MhlI GDGCHC 1 cut(s) 87
MlsI TGGCCA 1 cut(s) 323
MluCI AATT 2 cut(s) 123, 541
MluNI TGGCCA 1 cut(s) 323
MlyI GAGTC 1 cut(s) 251
MnlI CCTC 3 cut(s) 188, 502, 523
Mox20I TGGCCA 1 cut(s) 323
MroI TCCGGA 1 cut(s) 170
MroXI GAANNNNTTC 1 cut(s) 266
MscI TGGCCA 1 cut(s) 323
MseI TTAA 2 cut(s) 21, 544
MslI CAYNNNNRTG 1 cut(s) 329
Msp20I TGGCCA 1 cut(s) 323
MspI CCGG 3 cut(s) 80, 171, 297
MspR9I CCNGG 2 cut(s) 80, 212
Mva1269I GAATGC 1 cut(s) 73
MvaI CCWGG 1 cut(s) 212
MwoI GCNNNNNNNGC 3 cut(s) 234, 302, 378
NciI CCSGG 1 cut(s) 80
NdeII GATC 6 cut(s) 45, 141, 197, 288, 460, 517
NlaIII CATG 6 cut(s) 115, 153, 295, 334, 496, 509
NlaIV GGNNCC 2 cut(s) 84, 258
NmuCI GTSAC 2 cut(s) 25, 478
NspI RCATGY 1 cut(s) 153
NspV TTCGAA 1 cut(s) 270
PaeI GCATGC 1 cut(s) 153
PceI AGGCCT 1 cut(s) 512
PcsI WCGNNNNNNNCGW 2 cut(s) 47, 186
PctI GAATGC 1 cut(s) 73
PdmI GAANNNNTTC 1 cut(s) 266
PkrI GCNGC 1 cut(s) 109
PleI GAGTC 1 cut(s) 250
PpsI GAGTC 1 cut(s) 250
PpuMI RGGWCCY 2 cut(s) 208, 256
Psp5II RGGWCCY 2 cut(s) 208, 256
Psp6I CCWGG 1 cut(s) 210
PspGI CCWGG 1 cut(s) 210
PspN4I GGNNCC 2 cut(s) 84, 258
PspPI GGNCC 2 cut(s) 208, 256
PspPPI RGGWCCY 2 cut(s) 208, 256
PstNI CAGNNNCTG 1 cut(s) 211
RsaI GTAC 2 cut(s) 474, 502
RsaNI GTAC 2 cut(s) 473, 501
RseI CAYNNNNRTG 1 cut(s) 329
SaqAI TTAA 2 cut(s) 21, 544
SatI GCNGC 1 cut(s) 108
Sau3AI GATC 6 cut(s) 45, 141, 197, 288, 460, 517
Sau96I GGNCC 2 cut(s) 208, 256
SchI GAGTC 1 cut(s) 251
ScrFI CCNGG 2 cut(s) 80, 212
SduI GDGCHC 1 cut(s) 87
SfaNI GCATC 1 cut(s) 475
SfcI CTRYAG 1 cut(s) 382
SfuI TTCGAA 1 cut(s) 270
SinI GGWCC 2 cut(s) 208, 256
SmiMI CAYNNNNRTG 1 cut(s) 329
SphI GCATGC 1 cut(s) 153
Sse9I AATT 2 cut(s) 123, 541
SseBI AGGCCT 1 cut(s) 512
SsiI CCGC 2 cut(s) 228, 246
StuI AGGCCT 1 cut(s) 512
StyD4I CCNGG 2 cut(s) 78, 210
TaaI ACNGT 2 cut(s) 69, 500
TaiI ACGT 1 cut(s) 27
TaqI TCGA 2 cut(s) 180, 270
TasI AATT 2 cut(s) 123, 541
Tru1I TTAA 2 cut(s) 21, 544
Tru9I TTAA 2 cut(s) 21, 544
TscAI CASTG 2 cut(s) 411, 463
TseFI GTSAC 2 cut(s) 25, 478
TseI GCWGC 1 cut(s) 107
Tsp45I GTSAC 2 cut(s) 25, 478
TspDTI ATGAA 1 cut(s) 336
TspGWI ACGGA 1 cut(s) 210
TspRI CASTG 2 cut(s) 411, 463
VpaK11BI GGWCC 2 cut(s) 208, 256
XceI RCATGY 1 cut(s) 153
XmnI GAANNNNTTC 1 cut(s) 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.