MD16G1264600.v1.1

Protein GLUTAMINE DUMPER

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0020865
Physical Location & Seq
Forward (+)
33542495 .. 33543543
1049 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1264600.v1.1.491

Sequence Viewer

Length: 606 bp
ATGGCAGGGAGAGAGCCTTTTAACGTGACAGCCAGAGCACCGGAGATGACGACGGTACAGAGGTCGCCGTGGCATTCCCCGGTGCCCTACTTGTTCGGTGGGCTGGCAGCCATGTTGGGTTTAATTGCGTTTGCGCTTCTGATCTTAGCATGCTCCTACTGGAGGCTCTCCGGCTACCTCGAAAACCATGAAAACGGATCAGAGCGGGACCTGGAAGCCGGAGAAGGCGGCGAGTCCGCCCAGAAAGTCCCGCCGGTATTCGAAGAGAAAATCTTGGTGATCATGGCCGGAGACGCTAAGCCAACTTTCTTGGCCACACCCACGTCAAGTAGGTCTTCATCTTTTGGCGACAATACTAACAACACTTGTAGCTGTAGCCAAAAGACTGAAAAGTGGGTGAACGTGAGTGAGACGACTGCTGACAACAAGCAAGGAAGTAGTGATGGAAGTGATCAGCATCAGGTACAAGTTACTGATGAGAACAGGGAGAGTATGCATGAGGCCTCAGATCACTCGAATCAGAGAAAACCATTTATATATGAGAAAAAGAAGAAGATGGAGATGGATGCGTGTGGAACTTTGTATTGGGTGCAGAGAAGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

202

Amino Acids

22.37

Weight (kDa)

5.77

Isoelectric Point (pI)

54.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020865)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G57685
malus_domestica MD13G1263000.v1.1 MD16G1264600.v1.1
prunus_persica Prupe.1G094400_v2.0.a1
pyrus_communis pycom16g23390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 82
AccBSI CCGCTC 1 cut(s) 205
AciI CCGC 4 cut(s) 205, 228, 237, 251
AclWI GGATC 1 cut(s) 205
AcoI YGGCCR 2 cut(s) 285, 312
AfaI GTAC 2 cut(s) 57, 465
AfiI CCNNNNNNNGG 1 cut(s) 162
AjiI CACGTC 1 cut(s) 324
AjnI CCWGG 1 cut(s) 210
AjuI GAANNNNNNNTTGG 2 cut(s) 568, 600
AluBI AGCT 1 cut(s) 372
AluI AGCT 1 cut(s) 372
Alw21I GWGCWC 1 cut(s) 40
Alw26I GTCTC 2 cut(s) 285, 404
AlwI GGATC 1 cut(s) 205
AoxI GGCC 3 cut(s) 285, 312, 501
ApeKI GCWGC 1 cut(s) 107
AspLEI GCGC 1 cut(s) 136
AspS9I GGNCC 1 cut(s) 208
AsuC2I CCSGG 1 cut(s) 80
AsuHPI GGTGA 2 cut(s) 289, 409
AsuII TTCGAA 1 cut(s) 261
AvaII GGWCC 1 cut(s) 208
BaeGI GKGCMC 1 cut(s) 87
BaeI ACNNNNGTAYC 2 cut(s) 47, 80
BalI TGGCCA 1 cut(s) 314
BanI GGYRCC 1 cut(s) 82
BbsI GAAGAC 1 cut(s) 327
Bbv12I GWGCWC 1 cut(s) 40
BbvI GCAGC 1 cut(s) 119
BccI CCATC 3 cut(s) 437, 550, 556
BceAI ACGGC 1 cut(s) 52
BciT130I CCWGG 1 cut(s) 212
BclI TGATCA 2 cut(s) 279, 451
BcnI CCSGG 1 cut(s) 80
BcoDI GTCTC 2 cut(s) 285, 404
BfmI CTRYAG 1 cut(s) 373
BisI GCNGC 2 cut(s) 108, 229
BlpI GCTNAGC 1 cut(s) 297
BlsI GCNGC 2 cut(s) 109, 230
Bme1390I CCNGG 2 cut(s) 80, 212
Bme18I GGWCC 1 cut(s) 208
BmgBI CACGTC 1 cut(s) 324
BmgT120I GGNCC 1 cut(s) 208
BmiI GGNNCC 2 cut(s) 84, 209
BmrFI CCNGG 2 cut(s) 80, 212
BmsI GCATC 2 cut(s) 466, 556
BpiI GAAGAC 1 cut(s) 327
BpmI CTGGAG 1 cut(s) 181
Bpu1102I GCTNAGC 1 cut(s) 297
Bpu14I TTCGAA 1 cut(s) 261
BpuMI CCSGG 1 cut(s) 80
BsaBI GATNNNNATC 1 cut(s) 456
BsaJI CCNNGG 2 cut(s) 68, 78
BsaWI WCCGGW 1 cut(s) 40
Bsc4I CCNNNNNNNGG 1 cut(s) 162
Bse118I RCCGGY 1 cut(s) 253
Bse1I ACTGG 1 cut(s) 164
Bse8I GATNNNNATC 1 cut(s) 456
BseBI CCWGG 1 cut(s) 212
BseDI CCNNGG 2 cut(s) 68, 78
BseGI GGATG 1 cut(s) 571
BseJI GATNNNNATC 1 cut(s) 456
BseLI CCNNNNNNNGG 1 cut(s) 162
BseMII CTCAG 1 cut(s) 519
BseNI ACTGG 1 cut(s) 164
BseSI GKGCMC 1 cut(s) 87
BseXI GCAGC 1 cut(s) 119
BshFI GGCC 3 cut(s) 287, 314, 503
BshNI GGYRCC 1 cut(s) 82
BsiHKAI GWGCWC 1 cut(s) 40
BsiSI CCGG 6 cut(s) 41, 80, 171, 219, 254, 288
BslFI GGGAC 2 cut(s) 221, 233
BslI CCNNNNNNNGG 1 cut(s) 162
BsmAI GTCTC 2 cut(s) 285, 404
BsmBI CGTCTC 2 cut(s) 285, 404
BsmFI GGGAC 2 cut(s) 221, 233
BsmI GAATGC 1 cut(s) 73
BsnI GGCC 3 cut(s) 287, 314, 503
Bsp119I TTCGAA 1 cut(s) 261
Bsp1286I GDGCHC 2 cut(s) 40, 87
Bsp143I GATC 5 cut(s) 141, 197, 279, 451, 508
Bsp1720I GCTNAGC 1 cut(s) 297
BspACI CCGC 4 cut(s) 205, 228, 237, 251
BspANI GGCC 3 cut(s) 287, 314, 503
BspCNI CTCAG 1 cut(s) 518
BspLI GGNNCC 2 cut(s) 84, 209
BspPI GGATC 1 cut(s) 205
BspT104I TTCGAA 1 cut(s) 261
BspT107I GGYRCC 1 cut(s) 82
BsrBI CCGCTC 1 cut(s) 205
BsrFI RCCGGY 1 cut(s) 253
BsrI ACTGG 1 cut(s) 164
BssAI RCCGGY 1 cut(s) 253
BssECI CCNNGG 2 cut(s) 68, 78
BssMI GATC 5 cut(s) 141, 197, 279, 451, 508
Bst2UI CCWGG 1 cut(s) 212
Bst4CI ACNGT 1 cut(s) 55
Bst6I CTCTTC 1 cut(s) 258
BstBI TTCGAA 1 cut(s) 261
BstC8I GCNNGC 2 cut(s) 105, 151
BstDEI CTNAG 3 cut(s) 145, 297, 505
BstDSI CCRYGG 1 cut(s) 68
BstENI CCTNNNNNAGG 1 cut(s) 160
BstF5I GGATG 1 cut(s) 571
BstHHI GCGC 1 cut(s) 136
BstKTI GATC 5 cut(s) 144, 200, 282, 454, 511
BstMAI GTCTC 2 cut(s) 285, 404
BstMBI GATC 5 cut(s) 141, 197, 279, 451, 508
BstMWI GCNNNNNNNGC 1 cut(s) 293
BstNI CCWGG 1 cut(s) 212
BstNSI RCATGY 1 cut(s) 153
BstSCI CCNGG 2 cut(s) 78, 210
BstSFI CTRYAG 1 cut(s) 373
BstSLI GKGCMC 1 cut(s) 87
BstV1I GCAGC 1 cut(s) 119
BstV2I GAAGAC 1 cut(s) 327
BsuRI GGCC 3 cut(s) 287, 314, 503
BtgI CCRYGG 1 cut(s) 68
BtrI CACGTC 1 cut(s) 324
BtsCI GGATG 1 cut(s) 571
Cac8I GCNNGC 2 cut(s) 105, 151
CfoI GCGC 1 cut(s) 136
Cfr10I RCCGGY 1 cut(s) 253
Cfr13I GGNCC 1 cut(s) 208
CseI GACGC 1 cut(s) 302
Csp6I GTAC 2 cut(s) 56, 464
CviAII CATG 5 cut(s) 112, 150, 188, 283, 497
CviQI GTAC 2 cut(s) 56, 464
DdeI CTNAG 3 cut(s) 145, 297, 505
DpnI GATC 5 cut(s) 143, 199, 281, 453, 510
DpnII GATC 5 cut(s) 141, 197, 279, 451, 508
EaeI YGGCCR 2 cut(s) 285, 312
Eam1104I CTCTTC 1 cut(s) 258
EarI CTCTTC 1 cut(s) 258
EciI GGCGGA 1 cut(s) 226
Eco147I AGGCCT 1 cut(s) 503
Eco47I GGWCC 1 cut(s) 208
EcoNI CCTNNNNNAGG 1 cut(s) 160
EcoO109I RGGNCCY 1 cut(s) 208
EcoRII CCWGG 1 cut(s) 210
EcoT22I ATGCAT 1 cut(s) 498
Esp3I CGTCTC 2 cut(s) 285, 404
FaeI CATG 5 cut(s) 115, 153, 191, 286, 500
FaiI YATR 9 cut(s) 113, 151, 189, 284, 494, 498, 536, 538, 540
FalI AAGNNNNNCTT 2 cut(s) 319, 351
FaqI GGGAC 2 cut(s) 221, 233
FatI CATG 5 cut(s) 111, 149, 187, 282, 496
FauI CCCGC 2 cut(s) 198, 258
FbaI TGATCA 2 cut(s) 279, 451
Fnu4HI GCNGC 2 cut(s) 108, 229
FokI GGATG 1 cut(s) 578
Fsp4HI GCNGC 2 cut(s) 108, 229
GlaI GCGC 1 cut(s) 135
GluI GCNGC 2 cut(s) 108, 229
GsuI CTGGAG 1 cut(s) 181
HaeIII GGCC 3 cut(s) 287, 314, 503
HapII CCGG 6 cut(s) 41, 80, 171, 219, 254, 288
HgaI GACGC 1 cut(s) 302
HhaI GCGC 1 cut(s) 136
Hin1II CATG 5 cut(s) 115, 153, 191, 286, 500
Hin6I GCGC 1 cut(s) 134
HinP1I GCGC 1 cut(s) 134
HinfI GANTC 2 cut(s) 233, 517
HpaII CCGG 6 cut(s) 41, 80, 171, 219, 254, 288
HphI GGTGA 2 cut(s) 289, 409
Hpy166II GTNNAC 1 cut(s) 400
Hpy188I TCNGA 4 cut(s) 141, 202, 508, 522
Hpy8I GTNNAC 1 cut(s) 400
Hpy99I CGWCG 1 cut(s) 55
HpyAV CCTTC 1 cut(s) 218
HpyCH4III ACNGT 1 cut(s) 55
HpyCH4IV ACGT 3 cut(s) 24, 323, 402
HpyCH4V TGCA 2 cut(s) 496, 592
HpyF10VI GCNNNNNNNGC 1 cut(s) 293
HpyF3I CTNAG 3 cut(s) 145, 297, 505
HpySE526I ACGT 3 cut(s) 24, 323, 402
Hsp92II CATG 5 cut(s) 115, 153, 191, 286, 500
HspAI GCGC 1 cut(s) 134
Ksp22I TGATCA 2 cut(s) 279, 451
Kzo9I GATC 5 cut(s) 141, 197, 279, 451, 508
LmnI GCTCC 1 cut(s) 158
Lsp1109I GCAGC 1 cut(s) 119
LweI GCATC 2 cut(s) 466, 556
MaeII ACGT 3 cut(s) 24, 323, 402
MaeIII GTNAC 2 cut(s) 25, 469
MalI GATC 5 cut(s) 143, 199, 281, 453, 510
MbiI CCGCTC 1 cut(s) 205
MboI GATC 5 cut(s) 141, 197, 279, 451, 508
MboII GAAGA 4 cut(s) 275, 327, 562, 565
MhlI GDGCHC 2 cut(s) 40, 87
MlsI TGGCCA 1 cut(s) 314
MluCI AATT 1 cut(s) 123
MluNI TGGCCA 1 cut(s) 314
MlyI GAGTC 1 cut(s) 242
MnlI CCTC 5 cut(s) 54, 156, 188, 493, 514
Mox20I TGGCCA 1 cut(s) 314
Mph1103I ATGCAT 1 cut(s) 498
MscI TGGCCA 1 cut(s) 314
MseI TTAA 2 cut(s) 21, 122
Msp20I TGGCCA 1 cut(s) 314
MspI CCGG 6 cut(s) 41, 80, 171, 219, 254, 288
MspR9I CCNGG 2 cut(s) 80, 212
Mva1269I GAATGC 1 cut(s) 73
MvaI CCWGG 1 cut(s) 212
MwoI GCNNNNNNNGC 1 cut(s) 293
NciI CCSGG 1 cut(s) 80
NdeII GATC 5 cut(s) 141, 197, 279, 451, 508
NlaIII CATG 5 cut(s) 115, 153, 191, 286, 500
NlaIV GGNNCC 2 cut(s) 84, 209
NmuCI GTSAC 1 cut(s) 25
NsiI ATGCAT 1 cut(s) 498
NspI RCATGY 1 cut(s) 153
NspV TTCGAA 1 cut(s) 261
PaeI GCATGC 1 cut(s) 153
PceI AGGCCT 1 cut(s) 503
PctI GAATGC 1 cut(s) 73
PfeI GAWTC 1 cut(s) 517
PkrI GCNGC 2 cut(s) 109, 230
PleI GAGTC 1 cut(s) 241
PpsI GAGTC 1 cut(s) 241
PpuMI RGGWCCY 1 cut(s) 208
Psp5II RGGWCCY 1 cut(s) 208
Psp6I CCWGG 1 cut(s) 210
PspGI CCWGG 1 cut(s) 210
PspN4I GGNNCC 2 cut(s) 84, 209
PspPI GGNCC 1 cut(s) 208
PspPPI RGGWCCY 1 cut(s) 208
RsaI GTAC 2 cut(s) 57, 465
RsaNI GTAC 2 cut(s) 56, 464
SaqAI TTAA 2 cut(s) 21, 122
SatI GCNGC 2 cut(s) 108, 229
Sau3AI GATC 5 cut(s) 141, 197, 279, 451, 508
Sau96I GGNCC 1 cut(s) 208
SchI GAGTC 1 cut(s) 242
ScrFI CCNGG 2 cut(s) 80, 212
SduI GDGCHC 2 cut(s) 40, 87
SetI ASST 9 cut(s) 27, 65, 180, 213, 326, 335, 374, 405, 465
SfaNI GCATC 2 cut(s) 466, 556
SfcI CTRYAG 1 cut(s) 373
SfuI TTCGAA 1 cut(s) 261
SinI GGWCC 1 cut(s) 208
SphI GCATGC 1 cut(s) 153
Sse9I AATT 1 cut(s) 123
SseBI AGGCCT 1 cut(s) 503
SsiI CCGC 4 cut(s) 205, 228, 237, 251
StuI AGGCCT 1 cut(s) 503
StyD4I CCNGG 2 cut(s) 78, 210
TaaI ACNGT 1 cut(s) 55
TaiI ACGT 3 cut(s) 27, 326, 405
TaqI TCGA 3 cut(s) 180, 261, 515
TasI AATT 1 cut(s) 123
TauI GCSGC 1 cut(s) 231
TfiI GAWTC 1 cut(s) 517
Tru1I TTAA 2 cut(s) 21, 122
Tru9I TTAA 2 cut(s) 21, 122
TseFI GTSAC 1 cut(s) 25
TseI GCWGC 1 cut(s) 107
Tsp45I GTSAC 1 cut(s) 25
TspDTI ATGAA 2 cut(s) 204, 327
TspGWI ACGGA 1 cut(s) 210
VpaK11BI GGWCC 1 cut(s) 208
XagI CCTNNNNNAGG 1 cut(s) 160
XceI RCATGY 1 cut(s) 153
Zsp2I ATGCAT 1 cut(s) 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.