pycom16g23390

Protein GLUTAMINE DUMPER

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0020865
Physical Location & Seq
Forward (+)
24301310 .. 24301846
537 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g23390.1

Sequence Viewer

Length: 537 bp
ATGGCAGGGAGAGAGCCTTTTAACGTGACAGCCAGAGCACCGGAGATGACGACGGCACAGAGGTCGCCGTGGCATTCCCCGGTGCCCTACTTGTTCGGTGGGCTGGCAGCCATGTTGGGTTTAATTGCGTTTGCGCTTCTGATCTTAGCATGCTCCTACTGGAGGCTCTCCGGCTACCTCGAAAACCACGAAAACGGATCAGAACGGGACCTGGAAGCCGGAGAAGGCGACGAGTCCGCCCAGAAAGTCCCGCCGGTATTCGAAGAGAAAATCTTGGTGATCATGGCCGGAGACGCTAAGCCAACTTTCTTGGCCACACCCATGTCGAGCAGGTCTTCATCTTTTGGCGACAATACTAACAACACTTGTAGCTGTAGCCAAAAGACTGAAAAGTGGGTGGACGTGAGTGAGACGACTGCTGGCAACAAGCAAGGAAATAGTCATGGAAGTGATCAGCATCAGGTACAAGTTACTGATGAAAAGGGGGAGAGTGCCCAGGAGGCCTCAGATCACTCGGATCAGAGAGTAAGTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

179

Amino Acids

19.28

Weight (kDa)

4.87

Isoelectric Point (pI)

47.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020865)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G57685
malus_domestica MD13G1263000.v1.1 MD16G1264600.v1.1
prunus_persica Prupe.1G094400_v2.0.a1
pyrus_communis pycom16g23390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 321
AccB1I GGYRCC 1 cut(s) 82
AciI CCGC 2 cut(s) 237, 251
AclWI GGATC 2 cut(s) 205, 525
AcoI YGGCCR 2 cut(s) 285, 312
AfaI GTAC 1 cut(s) 465
AfiI CCNNNNNNNGG 1 cut(s) 162
AjiI CACGTC 1 cut(s) 403
AjnI CCWGG 2 cut(s) 210, 495
AluBI AGCT 1 cut(s) 372
AluI AGCT 1 cut(s) 372
Alw21I GWGCWC 1 cut(s) 40
Alw26I GTCTC 2 cut(s) 285, 404
AlwI GGATC 2 cut(s) 205, 525
AoxI GGCC 3 cut(s) 285, 312, 501
ApeKI GCWGC 1 cut(s) 107
AspLEI GCGC 1 cut(s) 136
AspS9I GGNCC 1 cut(s) 208
AsuC2I CCSGG 1 cut(s) 80
AsuHPI GGTGA 1 cut(s) 289
AsuII TTCGAA 1 cut(s) 261
AvaII GGWCC 1 cut(s) 208
BaeGI GKGCMC 2 cut(s) 87, 496
BalI TGGCCA 1 cut(s) 314
BanI GGYRCC 1 cut(s) 82
BbsI GAAGAC 1 cut(s) 327
Bbv12I GWGCWC 1 cut(s) 40
BbvI GCAGC 1 cut(s) 119
BceAI ACGGC 2 cut(s) 52, 69
BcgI CGANNNNNNTGC 2 cut(s) 45, 79
BciT130I CCWGG 2 cut(s) 212, 497
BclI TGATCA 2 cut(s) 279, 451
BcnI CCSGG 1 cut(s) 80
BcoDI GTCTC 2 cut(s) 285, 404
BfmI CTRYAG 1 cut(s) 373
BfuAI ACCTGC 1 cut(s) 321
BglI GCCNNNNNGGC 1 cut(s) 500
BisI GCNGC 1 cut(s) 108
BlpI GCTNAGC 1 cut(s) 297
BlsI GCNGC 1 cut(s) 109
Bme1390I CCNGG 3 cut(s) 80, 212, 497
Bme18I GGWCC 1 cut(s) 208
BmgBI CACGTC 1 cut(s) 403
BmgT120I GGNCC 1 cut(s) 208
BmiI GGNNCC 2 cut(s) 84, 209
BmrFI CCNGG 3 cut(s) 80, 212, 497
BmsI GCATC 1 cut(s) 466
BpiI GAAGAC 1 cut(s) 327
BpmI CTGGAG 1 cut(s) 181
Bpu1102I GCTNAGC 1 cut(s) 297
Bpu14I TTCGAA 1 cut(s) 261
BpuMI CCSGG 1 cut(s) 80
BsaBI GATNNNNATC 1 cut(s) 456
BsaJI CCNNGG 3 cut(s) 68, 78, 495
BsaWI WCCGGW 1 cut(s) 40
Bsc4I CCNNNNNNNGG 1 cut(s) 162
Bse118I RCCGGY 1 cut(s) 253
Bse1I ACTGG 1 cut(s) 164
Bse8I GATNNNNATC 1 cut(s) 456
BseBI CCWGG 2 cut(s) 212, 497
BseDI CCNNGG 3 cut(s) 68, 78, 495
BseJI GATNNNNATC 1 cut(s) 456
BseLI CCNNNNNNNGG 1 cut(s) 162
BseMII CTCAG 1 cut(s) 519
BseNI ACTGG 1 cut(s) 164
BseSI GKGCMC 2 cut(s) 87, 496
BseXI GCAGC 1 cut(s) 119
BshFI GGCC 3 cut(s) 287, 314, 503
BshNI GGYRCC 1 cut(s) 82
BsiHKAI GWGCWC 1 cut(s) 40
BsiSI CCGG 6 cut(s) 41, 80, 171, 219, 254, 288
BslFI GGGAC 2 cut(s) 221, 233
BslI CCNNNNNNNGG 1 cut(s) 162
BsmAI GTCTC 2 cut(s) 285, 404
BsmBI CGTCTC 2 cut(s) 285, 404
BsmFI GGGAC 2 cut(s) 221, 233
BsmI GAATGC 1 cut(s) 73
BsnI GGCC 3 cut(s) 287, 314, 503
Bsp119I TTCGAA 1 cut(s) 261
Bsp1286I GDGCHC 3 cut(s) 40, 87, 496
Bsp143I GATC 6 cut(s) 141, 197, 279, 451, 508, 517
Bsp1720I GCTNAGC 1 cut(s) 297
BspACI CCGC 2 cut(s) 237, 251
BspANI GGCC 3 cut(s) 287, 314, 503
BspCNI CTCAG 1 cut(s) 518
BspLI GGNNCC 2 cut(s) 84, 209
BspMI ACCTGC 1 cut(s) 321
BspPI GGATC 2 cut(s) 205, 525
BspT104I TTCGAA 1 cut(s) 261
BspT107I GGYRCC 1 cut(s) 82
BsrFI RCCGGY 1 cut(s) 253
BsrI ACTGG 1 cut(s) 164
BssAI RCCGGY 1 cut(s) 253
BssECI CCNNGG 3 cut(s) 68, 78, 495
BssMI GATC 6 cut(s) 141, 197, 279, 451, 508, 517
Bst2UI CCWGG 2 cut(s) 212, 497
Bst6I CTCTTC 1 cut(s) 258
BstBI TTCGAA 1 cut(s) 261
BstC8I GCNNGC 3 cut(s) 105, 151, 421
BstDEI CTNAG 3 cut(s) 145, 297, 505
BstDSI CCRYGG 1 cut(s) 68
BstENI CCTNNNNNAGG 1 cut(s) 160
BstHHI GCGC 1 cut(s) 136
BstKTI GATC 6 cut(s) 144, 200, 282, 454, 511, 520
BstMAI GTCTC 2 cut(s) 285, 404
BstMBI GATC 6 cut(s) 141, 197, 279, 451, 508, 517
BstMWI GCNNNNNNNGC 2 cut(s) 293, 500
BstNI CCWGG 2 cut(s) 212, 497
BstNSI RCATGY 1 cut(s) 153
BstSCI CCNGG 3 cut(s) 78, 210, 495
BstSFI CTRYAG 1 cut(s) 373
BstSLI GKGCMC 2 cut(s) 87, 496
BstV1I GCAGC 1 cut(s) 119
BstV2I GAAGAC 1 cut(s) 327
BsuRI GGCC 3 cut(s) 287, 314, 503
BtgI CCRYGG 1 cut(s) 68
BtrI CACGTC 1 cut(s) 403
BveI ACCTGC 1 cut(s) 321
Cac8I GCNNGC 3 cut(s) 105, 151, 421
CfoI GCGC 1 cut(s) 136
Cfr10I RCCGGY 1 cut(s) 253
Cfr13I GGNCC 1 cut(s) 208
CseI GACGC 1 cut(s) 302
Csp6I GTAC 1 cut(s) 464
CviAII CATG 5 cut(s) 112, 150, 283, 322, 443
CviQI GTAC 1 cut(s) 464
DdeI CTNAG 3 cut(s) 145, 297, 505
DpnI GATC 6 cut(s) 143, 199, 281, 453, 510, 519
DpnII GATC 6 cut(s) 141, 197, 279, 451, 508, 517
EaeI YGGCCR 2 cut(s) 285, 312
Eam1104I CTCTTC 1 cut(s) 258
EarI CTCTTC 1 cut(s) 258
EciI GGCGGA 1 cut(s) 226
Eco147I AGGCCT 1 cut(s) 503
Eco47I GGWCC 1 cut(s) 208
EcoNI CCTNNNNNAGG 1 cut(s) 160
EcoO109I RGGNCCY 1 cut(s) 208
EcoRII CCWGG 2 cut(s) 210, 495
Esp3I CGTCTC 2 cut(s) 285, 404
FaeI CATG 5 cut(s) 115, 153, 286, 325, 446
FaiI YATR 5 cut(s) 113, 151, 284, 323, 444
FaqI GGGAC 2 cut(s) 221, 233
FatI CATG 5 cut(s) 111, 149, 282, 321, 442
FauI CCCGC 1 cut(s) 258
FbaI TGATCA 2 cut(s) 279, 451
Fnu4HI GCNGC 1 cut(s) 108
Fsp4HI GCNGC 1 cut(s) 108
GlaI GCGC 1 cut(s) 135
GluI GCNGC 1 cut(s) 108
GsuI CTGGAG 1 cut(s) 181
HaeIII GGCC 3 cut(s) 287, 314, 503
HapII CCGG 6 cut(s) 41, 80, 171, 219, 254, 288
HgaI GACGC 1 cut(s) 302
HhaI GCGC 1 cut(s) 136
Hin1II CATG 5 cut(s) 115, 153, 286, 325, 446
Hin6I GCGC 1 cut(s) 134
HinP1I GCGC 1 cut(s) 134
HinfI GANTC 1 cut(s) 233
HpaII CCGG 6 cut(s) 41, 80, 171, 219, 254, 288
HphI GGTGA 1 cut(s) 289
Hpy166II GTNNAC 1 cut(s) 400
Hpy188I TCNGA 5 cut(s) 141, 202, 508, 517, 522
Hpy8I GTNNAC 1 cut(s) 400
Hpy99I CGWCG 2 cut(s) 55, 233
HpyAV CCTTC 1 cut(s) 218
HpyCH4IV ACGT 2 cut(s) 24, 402
HpyF10VI GCNNNNNNNGC 2 cut(s) 293, 500
HpyF3I CTNAG 3 cut(s) 145, 297, 505
HpySE526I ACGT 2 cut(s) 24, 402
Hsp92II CATG 5 cut(s) 115, 153, 286, 325, 446
HspAI GCGC 1 cut(s) 134
Ksp22I TGATCA 2 cut(s) 279, 451
Kzo9I GATC 6 cut(s) 141, 197, 279, 451, 508, 517
LmnI GCTCC 1 cut(s) 158
Lsp1109I GCAGC 1 cut(s) 119
LweI GCATC 1 cut(s) 466
MaeII ACGT 2 cut(s) 24, 402
MaeIII GTNAC 2 cut(s) 25, 469
MalI GATC 6 cut(s) 143, 199, 281, 453, 510, 519
MboI GATC 6 cut(s) 141, 197, 279, 451, 508, 517
MboII GAAGA 2 cut(s) 275, 327
MhlI GDGCHC 3 cut(s) 40, 87, 496
MlsI TGGCCA 1 cut(s) 314
MluCI AATT 2 cut(s) 123, 532
MluNI TGGCCA 1 cut(s) 314
MlyI GAGTC 1 cut(s) 242
MnlI CCTC 5 cut(s) 54, 156, 188, 493, 514
Mox20I TGGCCA 1 cut(s) 314
MscI TGGCCA 1 cut(s) 314
MseI TTAA 3 cut(s) 21, 122, 535
MslI CAYNNNNRTG 2 cut(s) 320, 447
Msp20I TGGCCA 1 cut(s) 314
MspI CCGG 6 cut(s) 41, 80, 171, 219, 254, 288
MspR9I CCNGG 3 cut(s) 80, 212, 497
Mva1269I GAATGC 1 cut(s) 73
MvaI CCWGG 2 cut(s) 212, 497
MwoI GCNNNNNNNGC 2 cut(s) 293, 500
NciI CCSGG 1 cut(s) 80
NdeII GATC 6 cut(s) 141, 197, 279, 451, 508, 517
NlaIII CATG 5 cut(s) 115, 153, 286, 325, 446
NlaIV GGNNCC 2 cut(s) 84, 209
NmuCI GTSAC 1 cut(s) 25
NspI RCATGY 1 cut(s) 153
NspV TTCGAA 1 cut(s) 261
PaeI GCATGC 1 cut(s) 153
PceI AGGCCT 1 cut(s) 503
PcsI WCGNNNNNNNCGW 1 cut(s) 186
PctI GAATGC 1 cut(s) 73
PkrI GCNGC 1 cut(s) 109
PleI GAGTC 1 cut(s) 241
PpsI GAGTC 1 cut(s) 241
PpuMI RGGWCCY 1 cut(s) 208
Psp5II RGGWCCY 1 cut(s) 208
Psp6I CCWGG 2 cut(s) 210, 495
PspGI CCWGG 2 cut(s) 210, 495
PspN4I GGNNCC 2 cut(s) 84, 209
PspPI GGNCC 1 cut(s) 208
PspPPI RGGWCCY 1 cut(s) 208
RsaI GTAC 1 cut(s) 465
RsaNI GTAC 1 cut(s) 464
RseI CAYNNNNRTG 2 cut(s) 320, 447
SaqAI TTAA 3 cut(s) 21, 122, 535
SatI GCNGC 1 cut(s) 108
Sau3AI GATC 6 cut(s) 141, 197, 279, 451, 508, 517
Sau96I GGNCC 1 cut(s) 208
SchI GAGTC 1 cut(s) 242
ScrFI CCNGG 3 cut(s) 80, 212, 497
SduI GDGCHC 3 cut(s) 40, 87, 496
SetI ASST 8 cut(s) 27, 65, 180, 213, 335, 374, 405, 465
SfaNI GCATC 1 cut(s) 466
SfcI CTRYAG 1 cut(s) 373
SfuI TTCGAA 1 cut(s) 261
SinI GGWCC 1 cut(s) 208
SmiMI CAYNNNNRTG 2 cut(s) 320, 447
SphI GCATGC 1 cut(s) 153
Sse9I AATT 2 cut(s) 123, 532
SseBI AGGCCT 1 cut(s) 503
SsiI CCGC 2 cut(s) 237, 251
StuI AGGCCT 1 cut(s) 503
StyD4I CCNGG 3 cut(s) 78, 210, 495
TaiI ACGT 2 cut(s) 27, 405
TaqI TCGA 3 cut(s) 180, 261, 326
TasI AATT 2 cut(s) 123, 532
Tru1I TTAA 3 cut(s) 21, 122, 535
Tru9I TTAA 3 cut(s) 21, 122, 535
TseFI GTSAC 1 cut(s) 25
TseI GCWGC 1 cut(s) 107
Tsp45I GTSAC 1 cut(s) 25
TspDTI ATGAA 2 cut(s) 327, 492
TspGWI ACGGA 1 cut(s) 210
VpaK11BI GGWCC 1 cut(s) 208
XagI CCTNNNNNAGG 1 cut(s) 160
XceI RCATGY 1 cut(s) 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.