MD14G1001300.v1.1

Tryptophan/tyrosine permease family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0019590
Physical Location & Seq
Reverse (-)
272347 .. 273329
983 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1001300.v1.1.491

Sequence Viewer

Length: 690 bp
ATGTGCAAGATTGCTGGCAACACTGTAGCCGACGCTCGCAAGGCCATACTCATTGGTGGGGCTGTTCCATTGATCACGGTCTTGTCATGGAATTTGATTGTGTTGGGGCTTTCTGGATCCGGAACCAACGCCGCAGCACCCGGAGACCCTATATCCCTTCTGCTTTCCGTTAATCCATCTGCACTGTCTGCAGTGCAAGCATTTGCCTTCTCCGCTTTGGCAACGAGCTTGATCGGGTACGCTGTTAGCTTTCCGAAACAGCTTCTTGATACTGTGGAATTGATTGCAAAAATCAACCTCAAAAATAACAAAGTAGGAGTAGCTTCTTCTCAAGGCAATGGGAGGGCTTCTTTTACCACCGGTTCAGGTTCAGGGCGTTGTATTGATGCTTCTGATTCTAAACCGGGATGGAGCATTAATATGATTGTAATGCCGCTGGTGCTCGGTATTCCAGTGCTCATAGCATCTTTCTTTCCCTCCACCTTTTCCAGAGCTCTTGATTTCGCCGGACCTTGTGCAAACTGTTTTCTGTTTGGCATCCTTCCTCCAGCAATGGCTTACATACACCAATCGAGGAAGAAATCAAGTTGGTGCAGGTCATCCAACATTTTACCAGGGGGAAATGCTACACTTGCCAATCCTCTTTGGAATTGCTGTCATTTTGGGGATTTGGCATTAGCTAAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Trp_Tyr_perm

Protein Analysis

230

Amino Acids

23.64

Weight (kDa)

9.34

Isoelectric Point (pI)

34.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trp_Tyr_perm PF03222 1 - 209 6.2e-30 Tryptophan/tyrosine permease family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019590)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33260 AT2G33260
fragaria_vesca FvH4_2g12801
malus_domestica MD14G1001300.v1.1
prunus_persica Prupe.7G001500_v2.0.a1
pyrus_communis pycom12g00130 pycom49g00080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 585
AccIII TCCGGA 1 cut(s) 119
AciI CCGC 3 cut(s) 132, 213, 434
AclWI GGATC 2 cut(s) 111, 124
AcsI RAATTY 1 cut(s) 91
AfaI GTAC 1 cut(s) 239
AgeI ACCGGT 1 cut(s) 359
AjnI CCWGG 1 cut(s) 613
AluBI AGCT 6 cut(s) 228, 249, 262, 323, 494, 680
AluI AGCT 6 cut(s) 228, 249, 262, 323, 494, 680
Alw21I GWGCWC 3 cut(s) 444, 459, 496
Alw26I GTCTC 1 cut(s) 138
AlwI GGATC 2 cut(s) 111, 124
Aor13HI TCCGGA 1 cut(s) 119
AoxI GGCC 1 cut(s) 42
ApeKI GCWGC 1 cut(s) 134
ApoI RAATTY 1 cut(s) 91
AseI ATTAAT 1 cut(s) 417
AsiGI ACCGGT 1 cut(s) 359
AspS9I GGNCC 1 cut(s) 509
AsuC2I CCSGG 2 cut(s) 141, 405
AvaII GGWCC 1 cut(s) 509
BamHI GGATCC 1 cut(s) 116
BanII GRGCYC 1 cut(s) 496
BarI GAAGNNNNNNTAC 2 cut(s) 373, 405
Bbv12I GWGCWC 3 cut(s) 444, 459, 496
BbvI GCAGC 1 cut(s) 146
BccI CCATC 2 cut(s) 184, 402
BciT130I CCWGG 1 cut(s) 615
BclI TGATCA 1 cut(s) 72
BcnI CCSGG 2 cut(s) 141, 405
BcoDI GTCTC 1 cut(s) 138
BfmI CTRYAG 2 cut(s) 24, 189
BfuAI ACCTGC 1 cut(s) 585
BisI GCNGC 3 cut(s) 132, 135, 434
BlsI GCNGC 3 cut(s) 133, 136, 435
Bme1390I CCNGG 3 cut(s) 141, 405, 615
Bme18I GGWCC 1 cut(s) 509
BmgT120I GGNCC 1 cut(s) 509
BmiI GGNNCC 2 cut(s) 118, 124
BmrFI CCNGG 3 cut(s) 141, 405, 615
BmsI GCATC 3 cut(s) 376, 473, 546
BpmI CTGGAG 1 cut(s) 531
BpuEI CTTGAG 1 cut(s) 315
BpuMI CCSGG 2 cut(s) 141, 405
BsaI GGTCTC 1 cut(s) 138
BsaJI CCNNGG 1 cut(s) 614
BsaWI WCCGGW 2 cut(s) 119, 359
Bse118I RCCGGY 1 cut(s) 359
Bse1I ACTGG 1 cut(s) 452
Bse3DI GCAATG 2 cut(s) 343, 558
BseAI TCCGGA 1 cut(s) 119
BseBI CCWGG 1 cut(s) 615
BseDI CCNNGG 1 cut(s) 614
BseGI GGATG 3 cut(s) 413, 537, 599
BseMI GCAATG 2 cut(s) 343, 558
BseNI ACTGG 1 cut(s) 452
BseXI GCAGC 1 cut(s) 146
BsgI GTGCAG 2 cut(s) 165, 613
BshFI GGCC 1 cut(s) 44
BshTI ACCGGT 1 cut(s) 359
BsiHKAI GWGCWC 3 cut(s) 444, 459, 496
BsiSI CCGG 5 cut(s) 120, 141, 360, 404, 507
BsmAI GTCTC 1 cut(s) 138
BsnI GGCC 1 cut(s) 44
Bso31I GGTCTC 1 cut(s) 138
Bsp1286I GDGCHC 3 cut(s) 444, 459, 496
Bsp13I TCCGGA 1 cut(s) 119
Bsp143I GATC 3 cut(s) 72, 116, 231
BspACI CCGC 3 cut(s) 132, 213, 434
BspANI GGCC 1 cut(s) 44
BspEI TCCGGA 1 cut(s) 119
BspLI GGNNCC 2 cut(s) 118, 124
BspMAI CTGCAG 1 cut(s) 193
BspMI ACCTGC 1 cut(s) 585
BspPI GGATC 2 cut(s) 111, 124
BspTNI GGTCTC 1 cut(s) 138
BsrDI GCAATG 2 cut(s) 343, 558
BsrFI RCCGGY 1 cut(s) 359
BsrI ACTGG 1 cut(s) 452
BssAI RCCGGY 1 cut(s) 359
BssECI CCNNGG 1 cut(s) 614
BssMI GATC 3 cut(s) 72, 116, 231
Bst2UI CCWGG 1 cut(s) 615
Bst4CI ACNGT 5 cut(s) 25, 79, 186, 274, 524
BstAPI GCANNNNNTGC 1 cut(s) 188
BstC8I GCNNGC 3 cut(s) 16, 37, 198
BstF5I GGATG 3 cut(s) 413, 537, 599
BstKTI GATC 3 cut(s) 75, 119, 234
BstMAI GTCTC 1 cut(s) 138
BstMBI GATC 3 cut(s) 72, 116, 231
BstMWI GCNNNNNNNGC 6 cut(s) 41, 188, 197, 212, 439, 632
BstNI CCWGG 1 cut(s) 615
BstSCI CCNGG 3 cut(s) 139, 403, 613
BstSFI CTRYAG 2 cut(s) 24, 189
BstV1I GCAGC 1 cut(s) 146
BstX2I RGATCY 1 cut(s) 116
BstYI RGATCY 1 cut(s) 116
BsuRI GGCC 1 cut(s) 44
BtsCI GGATG 3 cut(s) 413, 537, 599
BtsI GCAGTG 1 cut(s) 198
BtsIMutI CAGTG 4 cut(s) 21, 182, 198, 459
BveI ACCTGC 1 cut(s) 585
Cac8I GCNNGC 3 cut(s) 16, 37, 198
Cfr10I RCCGGY 1 cut(s) 359
Cfr13I GGNCC 1 cut(s) 509
CseI GACGC 1 cut(s) 41
Csp6I GTAC 1 cut(s) 238
CspAI ACCGGT 1 cut(s) 359
CviAII CATG 1 cut(s) 87
CviQI GTAC 1 cut(s) 238
DpnI GATC 3 cut(s) 74, 118, 233
DpnII GATC 3 cut(s) 72, 116, 231
Ecl136II GAGCTC 1 cut(s) 494
Eco24I GRGCYC 1 cut(s) 496
Eco31I GGTCTC 1 cut(s) 138
Eco47I GGWCC 1 cut(s) 509
Eco53kI GAGCTC 1 cut(s) 494
EcoICRI GAGCTC 1 cut(s) 494
EcoRII CCWGG 1 cut(s) 613
EcoT38I GRGCYC 1 cut(s) 496
FaeI CATG 1 cut(s) 90
FaiI YATR 6 cut(s) 47, 88, 152, 422, 461, 563
FatI CATG 1 cut(s) 86
FbaI TGATCA 1 cut(s) 72
Fnu4HI GCNGC 3 cut(s) 132, 135, 434
FokI GGATG 3 cut(s) 420, 524, 586
FriOI GRGCYC 1 cut(s) 496
Fsp4HI GCNGC 3 cut(s) 132, 135, 434
GluI GCNGC 3 cut(s) 132, 135, 434
GsuI CTGGAG 1 cut(s) 531
HaeIII GGCC 1 cut(s) 44
HapII CCGG 5 cut(s) 120, 141, 360, 404, 507
HgaI GACGC 1 cut(s) 41
Hin1II CATG 1 cut(s) 90
HinfI GANTC 1 cut(s) 395
HpaII CCGG 5 cut(s) 120, 141, 360, 404, 507
Hpy188I TCNGA 2 cut(s) 255, 394
Hpy188III TCNNGA 6 cut(s) 114, 120, 266, 489, 497, 687
Hpy99I CGWCG 1 cut(s) 35
HpyAV CCTTC 3 cut(s) 167, 217, 551
HpyCH4III ACNGT 5 cut(s) 25, 79, 186, 274, 524
HpyCH4V TGCA 7 cut(s) 6, 182, 191, 196, 287, 518, 594
HpyF10VI GCNNNNNNNGC 6 cut(s) 41, 188, 197, 212, 439, 632
Hsp92II CATG 1 cut(s) 90
Kpn2I TCCGGA 1 cut(s) 119
Ksp22I TGATCA 1 cut(s) 72
Kzo9I GATC 3 cut(s) 72, 116, 231
LmnI GCTCC 1 cut(s) 411
Lsp1109I GCAGC 1 cut(s) 146
LweI GCATC 3 cut(s) 376, 473, 546
MalI GATC 3 cut(s) 74, 118, 233
MboI GATC 3 cut(s) 72, 116, 231
MboII GAAGA 2 cut(s) 318, 589
MflI RGATCY 1 cut(s) 116
MhlI GDGCHC 3 cut(s) 444, 459, 496
MluCI AATT 3 cut(s) 91, 278, 649
MmeI TCCRAC 1 cut(s) 627
MnlI CCTC 6 cut(s) 308, 336, 487, 555, 567, 651
MroI TCCGGA 1 cut(s) 119
MseI TTAA 2 cut(s) 171, 417
MslI CAYNNNNRTG 1 cut(s) 419
MspA1I CMGCKG 1 cut(s) 436
MspI CCGG 5 cut(s) 120, 141, 360, 404, 507
MspR9I CCNGG 3 cut(s) 141, 405, 615
MvaI CCWGG 1 cut(s) 615
MwoI GCNNNNNNNGC 6 cut(s) 41, 188, 197, 212, 439, 632
NciI CCSGG 2 cut(s) 141, 405
NdeII GATC 3 cut(s) 72, 116, 231
NlaIII CATG 1 cut(s) 90
NlaIV GGNNCC 2 cut(s) 118, 124
PfeI GAWTC 1 cut(s) 395
PinAI ACCGGT 1 cut(s) 359
PkrI GCNGC 3 cut(s) 133, 136, 435
PshBI ATTAAT 1 cut(s) 417
Psp124BI GAGCTC 1 cut(s) 496
Psp6I CCWGG 1 cut(s) 613
PspGI CCWGG 1 cut(s) 613
PspN4I GGNNCC 2 cut(s) 118, 124
PspPI GGNCC 1 cut(s) 509
PstI CTGCAG 1 cut(s) 193
PsuI RGATCY 1 cut(s) 116
RsaI GTAC 1 cut(s) 239
RsaNI GTAC 1 cut(s) 238
RseI CAYNNNNRTG 1 cut(s) 419
SacI GAGCTC 1 cut(s) 496
SaqAI TTAA 2 cut(s) 171, 417
SatI GCNGC 3 cut(s) 132, 135, 434
Sau3AI GATC 3 cut(s) 72, 116, 231
Sau96I GGNCC 1 cut(s) 509
ScrFI CCNGG 3 cut(s) 141, 405, 615
SduI GDGCHC 3 cut(s) 444, 459, 496
SfaNI GCATC 3 cut(s) 376, 473, 546
SfcI CTRYAG 2 cut(s) 24, 189
SinI GGWCC 1 cut(s) 509
SmiMI CAYNNNNRTG 1 cut(s) 419
SmlI CTYRAG 1 cut(s) 330
SmoI CTYRAG 1 cut(s) 330
Sse9I AATT 3 cut(s) 91, 278, 649
SsiI CCGC 3 cut(s) 132, 213, 434
SstI GAGCTC 1 cut(s) 496
StyD4I CCNGG 3 cut(s) 139, 403, 613
TaaI ACNGT 5 cut(s) 25, 79, 186, 274, 524
TaqI TCGA 1 cut(s) 572
TasI AATT 3 cut(s) 91, 278, 649
TauI GCSGC 2 cut(s) 134, 436
TfiI GAWTC 1 cut(s) 395
Tru1I TTAA 2 cut(s) 171, 417
Tru9I TTAA 2 cut(s) 171, 417
TscAI CASTG 4 cut(s) 28, 189, 198, 459
TseI GCWGC 1 cut(s) 134
TspGWI ACGGA 1 cut(s) 157
TspRI CASTG 4 cut(s) 28, 189, 198, 459
VpaK11BI GGWCC 1 cut(s) 509
VspI ATTAAT 1 cut(s) 417
XapI RAATTY 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.