MD14G1073100.v1.1

Retroviral aspartyl protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0020126
Physical Location & Seq
Forward (+)
8133287 .. 8139983
6697 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1073100.v1.1.491

Sequence Viewer

Length: 2772 bp
ATGCCTTTGGCAGTCGAATTAAGTGCTTCACAGACTTCAGATGGACCTAAAAATCTTGAGGTCCTTCACATGCTAAATGTGACTATAGCTTCTCTTGCTTCATATGTGTCTTTGGGAGATGAGGTTCCCTCGGGCACTGTCATGTTGGCAGCAGCCTTGTTAAAATCTTCTCTGTGTATACTTCATGCTGGTGAATCGACATCATGGATTAATAGTCTTCCTATAGTTTGTAGTTCTGTGGCAGATCTGATAAGCCAATTTGTGGATAAAAAAAGTTTGCTGGTGGGTGAAAGCCAACCATCTGATGATGAAGGACATGAGACTATGAAAGCAAGTGCACTAATATTAATATGTACTGTTTTTGAGGATGCCCTTAGCACTTGTAAAGGAGTGCCAAATGAGCATCTATTGGGCGTTATATCTGCATTGTTCCTCAAGCTCGTTTTGGCAGCTAATTTCATGATCTATCGGGCTGAGAAACAATGCCATTTTCAGAAATGTATTGGAACTGCTGTAATTGCTATTGGACCTGAGAGGATACTTGAACTTGTTCCTATATCTCTCAATGCTAGTGATTTCACCAGTTCAAACATTTGGTTGATACCTATTTTGAAAGATTATGTTGTTGGAGCATCACTTGGTTACTATATGGAGCATATTATGCCTCTTGCAAGGTCCTTTTGTAGGGCCAGTGGTCAAGATCTACAGGCTCATGCACGTGATCTGTTGGGATTGCTACCTGCTTTCTGCAATCTTCCAACTGATATATGCCACAAGTTTCGGCCATTGGCTGGGATTTTAGTAACATTTATTCAGGACTCCTTAATGCATGAAAATACTGCAGTTGCCTTGCAGGTTATTGTGAATCAGAACAAAAGTGTACTTGATCAAAAAGATGGTGCTGGTGACACAAGTTCCTATGCAGTAAACAAATCTTTACTAGAGTTCTGCAACATACCCACGTATTCAAAGAAAACTGCTACCAGAAACATTAAGGCTTTGACATCATGGCAAATCCAATCTTCCGTTGCGACGATCGGGTCGACAATGCAGAATTTGTCCGCTGTAATCGCTTCAATGGAGTCTCGATTTGCAGCCTTGGAATTTCATTTTTCTGGTGCTGATCGTCCTCATCGTGCTCCCGTGGATCATGGAAATTCTTCAAATCCAATGGGTAGTTCTGAAGAAACCTTGTCGGATCGAGTTGATCATATGGGTCCGCGTTTTTTCCGTCAAGATTCGTCGTTTCAACCAAGGCCGCCATTGATCAAGCTTGAGTTTCCTCGGTTCTCAGATGGTGATGATCCTTTGGCATGGGTTTATCGGGCAGAGCATTATTTCGATTATTTCTCGGTTGACGATGCCCAAAAGGTACGGATGGCCTCTTTTCATATGGACAACGAAGCTTTACAATGGTTTCAATGGAGGAATTGCATCAAGAATCACCCAACATGGACAGATTTTGTTCATGTCTTCTGTAAAGAATTTGGGCCTTCTGAATTTGAAGACTTTACCGAAGCCCTAGTTCAATTGAAGCAGTTGGGTTCCCTCAAAGAGTACGTCTCGGAGTTTCGTCGATTGGCGAATCGGACTACGGAGATAGGTCCAGTCATGCTACGTGGTTGTTTCATCGGCGGACTGAAACCAGAGTTACGTCATGACGTCAAATTGCTTCGCCCATCTGATGTACATGAAGCAATTGCGCTGGCCTTCCAATTGGACATCAAACTATCAGATACCAAGGTTCGAAATTTCTCCAGAAATTCTGCTTCTTCTTTCAGTAATGCTACCAACTCAAAATCTGTGCCTCTGTTATCAGGTAGTAATTCTTATTCTAGCACATCTAGGGCTAGTAATGTGAGAAAAATGTCCTTTGAAGAACTTCAAGATCGTCGGAAAAAGGGGTTGTGTTATTCTTGTCCAGAGAAATGGGTTCGGGGGCATGTTTGTGCTACTCAACAACTACTATTATTAGATGTCTCTGCTGATAGGATTGAAGATTTCGATGGTGAGGACCATGATGACCAACAGGTGGAAATCACAGCTTGTGCAGTTTTTGGCTCATCGGCTCCCCAACATATTCAGACTATGAAGGTCTCTGGATTAATCAAGAATTGCCCAGTTATTGTATTGTTGGATTCGGGTAGCTCACACAACTTTATTAGTTTGTCTATTGCCAAACAATTGGGGTGGAAGGTTGACTCAAAACATTCTTTTGAGGTGATGATTGCAAATGGGGGTACTATTTCCAGTAAAGGTTGTTGTCCTCAAATTAAGTTGCAGATTCAACAATATGAGTATATTTCTGATTTCTATGTACTACAATTGGGTGGTTGTGATGTGGTTTTGGGAGCTCAGTGGTTAAGAACTTTAGGTCCTATTCCTTGGGATTTTGACAAACTCAAAATGGAATTTACATTGGGCAAGAAGCACTATTGTATTTCTAGTTCTCCACCTCCTGCACCAACGTCCATTTCAACCTGTCAAGTGGAGAAATTGCTTTCTCAAGGTCCTTTTGGTGTAGTTCTTTTCTTCATAGAACCCGAGACTCAGGTTGCTATTGTTGGGGATCTCACTGTTCTTCAAGAAACTGAGTTAGATGGCTTATTATCTCGATTTGCTTCTGTATTCCAAGCACCAACCACACTTCCTCCTTCCCGTTCTCATGACCATAGAATCCCTCTTTTGGAAGGCAGCAAGCCTCTAAGTGCTAGGCCTTATAGGTATGGTCCCTTACAAAAATCTGAAATCGAAAAGTGTGTTCAGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag

Protein Analysis

924

Amino Acids

102.09

Weight (kDa)

6.0

Isoelectric Point (pI)

42.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRP12_HEAT PF08161 159 - 336 3.6e-24 Ribosomal RNA-processing protein 12, HEAT repeats
Ty3_capsid PF19259 451 - 584 1.1e-10 Ty3 transposon capsid-like protein
Retrotrans_gag PF03732 459 - 549 4.5e-11 Retrotransposon gag protein
RVP_2 PF08284 700 - 789 1.4e-14 Retroviral aspartyl protease
gag-asp_proteas PF13975 700 - 789 2.5e-06 gag-polyprotein putative aspartyl protease
Asp_protease_2 PF13650 700 - 784 3.7e-06 Aspartyl protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020126)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g23981 FvH4_4g10392
malus_domestica MD14G1073100.v1.1
pyrus_communis pycom01g01230
rosa_chinensis RchiOBHm_Chr7g0227361
rosa_roxburghii Rroxscaffold_6G00402460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1039
AatII GACGTC 1 cut(s) 1665
Acc36I ACCTGC 2 cut(s) 748, 844
AccB7I CCANNNNNTGG 3 cut(s) 262, 791, 2032
AccI GTMKAC 2 cut(s) 178, 1043
AccII CGCG 1 cut(s) 1222
AciI CCGC 4 cut(s) 1062, 1220, 1259, 1635
AclWI GGATC 4 cut(s) 1155, 1206, 1298, 2577
AcoI YGGCCR 1 cut(s) 782
AcsI RAATTY 8 cut(s) 1054, 1103, 1156, 1484, 1499, 1750, 1762, 2411
AcuI CTGAAG 2 cut(s) 21, 1203
AcvI CACGTG 1 cut(s) 719
AcyI GRCGYC 1 cut(s) 1662
AfaI GTAC 7 cut(s) 355, 882, 1374, 1559, 1689, 2242, 2319
AfiI CCNNNNNNNGG 5 cut(s) 262, 684, 791, 2032, 2686
AjuI GAANNNNNNNTTGG 4 cut(s) 1162, 1194, 2458, 2490
AloI GAACNNNNNNTCC 4 cut(s) 108, 140, 2359, 2391
AluBI AGCT 8 cut(s) 89, 439, 452, 1273, 1406, 2045, 2148, 2354
AluI AGCT 8 cut(s) 89, 439, 452, 1273, 1406, 2045, 2148, 2354
Alw21I GWGCWC 3 cut(s) 340, 1141, 2356
Alw26I GTCTC 6 cut(s) 314, 1089, 1567, 1984, 2101, 2540
Alw44I GTGCAC 1 cut(s) 336
AlwI GGATC 4 cut(s) 1155, 1206, 1298, 2577
Ama87I CYCGRG 2 cut(s) 130, 2543
AoxI GGCC 7 cut(s) 687, 782, 1256, 1380, 1490, 1707, 2714
ApaLI GTGCAC 1 cut(s) 336
ApeKI GCWGC 5 cut(s) 149, 152, 449, 1094, 2694
ApoI RAATTY 8 cut(s) 1054, 1103, 1156, 1484, 1499, 1750, 1762, 2411
ArsI GACNNNNNNTTYG 2 cut(s) 1545, 1577
AseI ATTAAT 3 cut(s) 210, 347, 2105
Asp700I GAANNNNTTC 3 cut(s) 549, 1159, 1881
AspLEI GCGC 1 cut(s) 1705
AsuHPI GGTGA 8 cut(s) 203, 299, 571, 917, 1310, 1436, 2021, 2233
AsuII TTCGAA 1 cut(s) 1747
AvaI CYCGRG 2 cut(s) 130, 2543
BaeGI GKGCMC 2 cut(s) 137, 340
BaeI ACNNNNGTAYC 2 cut(s) 1728, 1761
BanII GRGCYC 1 cut(s) 2356
BbrPI CACGTG 1 cut(s) 719
BbsI GAAGAC 3 cut(s) 209, 1465, 1512
Bbv12I GWGCWC 3 cut(s) 340, 1141, 2356
BbvI GCAGC 5 cut(s) 161, 164, 461, 1106, 2706
BccI CCATC 8 cut(s) 35, 307, 890, 1289, 1372, 1687, 2000, 2594
BcgI CGANNNNNNTGC 2 cut(s) 5, 39
BciVI GTATCC 1 cut(s) 531
BclI TGATCA 3 cut(s) 886, 1207, 1266
BcoDI GTCTC 6 cut(s) 314, 1089, 1567, 1984, 2101, 2540
BfaI CTAG 8 cut(s) 570, 941, 1523, 1836, 1845, 1851, 2445, 2712
BfmI CTRYAG 4 cut(s) 84, 222, 704, 840
BfuAI ACCTGC 2 cut(s) 748, 844
BfuI GTATCC 1 cut(s) 531
BglII AGATCT 2 cut(s) 244, 700
BisI GCNGC 6 cut(s) 150, 153, 450, 1095, 1259, 2695
BlsI GCNGC 6 cut(s) 151, 154, 451, 1096, 1260, 2696
BmeT110I CYCGRG 2 cut(s) 130, 2543
BmiI GGNNCC 5 cut(s) 126, 1218, 1546, 2070, 2731
BmrI ACTGGG 1 cut(s) 2114
BmsI GCATC 5 cut(s) 358, 412, 641, 1351, 1443
BmuI ACTGGG 1 cut(s) 2114
BpiI GAAGAC 3 cut(s) 209, 1465, 1512
BplI GAGNNNNNCTC 4 cut(s) 113, 145, 1547, 1579
BpmI CTGGAG 1 cut(s) 1741
Bpu10I CCTNAGC 1 cut(s) 374
Bpu14I TTCGAA 1 cut(s) 1747
BpuEI CTTGAG 4 cut(s) 77, 419, 1295, 2490
BsaAI YACGTR 3 cut(s) 719, 963, 1619
BsaHI GRCGYC 1 cut(s) 1662
BsaI GGTCTC 1 cut(s) 2101
BsaJI CCNNGG 7 cut(s) 129, 1098, 1143, 1253, 1283, 1740, 2384
BsaXI ACNNNNNCTCC 4 cut(s) 108, 138, 2635, 2665
Bsc4I CCNNNNNNNGG 5 cut(s) 262, 684, 791, 2032, 2686
Bse1I ACTGG 5 cut(s) 582, 690, 1607, 2120, 2250
BseDI CCNNGG 7 cut(s) 129, 1098, 1143, 1253, 1283, 1740, 2384
BseGI GGATG 2 cut(s) 373, 1383
BseLI CCNNNNNNNGG 5 cut(s) 262, 684, 791, 2032, 2686
BseMII CTCAG 6 cut(s) 465, 522, 1305, 2369, 2564, 2583
BseNI ACTGG 5 cut(s) 582, 690, 1607, 2120, 2250
BseSI GKGCMC 2 cut(s) 137, 340
BseXI GCAGC 5 cut(s) 161, 164, 461, 1106, 2706
BseYI CCCAGC 1 cut(s) 791
BsgI GTGCAG 2 cut(s) 2070, 2445
Bsh1236I CGCG 1 cut(s) 1222
Bsh1285I CGRYCG 1 cut(s) 1038
BshFI GGCC 7 cut(s) 689, 784, 1258, 1382, 1492, 1709, 2716
BsiEI CGRYCG 1 cut(s) 1038
BsiHKAI GWGCWC 3 cut(s) 340, 1141, 2356
BsiHKCI CYCGRG 2 cut(s) 130, 2543
BslFI GGGAC 1 cut(s) 2715
BslI CCNNNNNNNGG 5 cut(s) 262, 684, 791, 2032, 2686
BsmAI GTCTC 6 cut(s) 314, 1089, 1567, 1984, 2101, 2540
BsmBI CGTCTC 1 cut(s) 1567
BsmFI GGGAC 1 cut(s) 2715
BsnI GGCC 7 cut(s) 689, 784, 1258, 1382, 1492, 1709, 2716
Bso31I GGTCTC 1 cut(s) 2101
BsoBI CYCGRG 2 cut(s) 130, 2543
Bsp119I TTCGAA 1 cut(s) 1747
Bsp1286I GDGCHC 4 cut(s) 137, 340, 1141, 2356
Bsp1407I TGTACA 1 cut(s) 1687
BspACI CCGC 4 cut(s) 1062, 1220, 1259, 1635
BspANI GGCC 7 cut(s) 689, 784, 1258, 1382, 1492, 1709, 2716
BspCNI CTCAG 6 cut(s) 466, 523, 1304, 2368, 2563, 2584
BspFNI CGCG 1 cut(s) 1222
BspHI TCATGA 3 cut(s) 459, 1657, 2665
BspLI GGNNCC 5 cut(s) 126, 1218, 1546, 2070, 2731
BspMAI CTGCAG 1 cut(s) 844
BspMI ACCTGC 2 cut(s) 748, 844
BspPI GGATC 4 cut(s) 1155, 1206, 1298, 2577
BspT104I TTCGAA 1 cut(s) 1747
BspTNI GGTCTC 1 cut(s) 2101
BsrGI TGTACA 1 cut(s) 1687
BsrI ACTGG 5 cut(s) 582, 690, 1607, 2120, 2250
BssECI CCNNGG 7 cut(s) 129, 1098, 1143, 1253, 1283, 1740, 2384
BssNAI GTATAC 1 cut(s) 179
BssNI GRCGYC 1 cut(s) 1662
BssT1I CCWWGG 4 cut(s) 1098, 1253, 1740, 2384
Bst1107I GTATAC 1 cut(s) 179
Bst4CI ACNGT 3 cut(s) 139, 358, 2578
BstACI GRCGYC 1 cut(s) 1662
BstAPI GCANNNNNTGC 1 cut(s) 661
BstAUI TGTACA 1 cut(s) 1687
BstBAI YACGTR 3 cut(s) 719, 963, 1619
BstBI TTCGAA 1 cut(s) 1747
BstC8I GCNNGC 2 cut(s) 1707, 2699
BstDEI CTNAG 8 cut(s) 374, 474, 531, 1291, 2355, 2550, 2592, 2705
BstDSI CCRYGG 1 cut(s) 1143
BstENI CCTNNNNNAGG 1 cut(s) 682
BstF5I GGATG 2 cut(s) 373, 1383
BstFNI CGCG 1 cut(s) 1222
BstHHI GCGC 1 cut(s) 1705
BstMAI GTCTC 6 cut(s) 314, 1089, 1567, 1984, 2101, 2540
BstMCI CGRYCG 1 cut(s) 1038
BstMWI GCNNNNNNNGC 5 cut(s) 95, 400, 518, 661, 1070
BstNSI RCATGY 2 cut(s) 73, 1946
BstSFI CTRYAG 4 cut(s) 84, 222, 704, 840
BstSLI GKGCMC 2 cut(s) 137, 340
BstUI CGCG 1 cut(s) 1222
BstV1I GCAGC 5 cut(s) 161, 164, 461, 1106, 2706
BstV2I GAAGAC 3 cut(s) 209, 1465, 1512
BstX2I RGATCY 3 cut(s) 244, 700, 2569
BstXI CCANNNNNNTGG 2 cut(s) 1929, 2185
BstYI RGATCY 3 cut(s) 244, 700, 2569
BstZ17I GTATAC 1 cut(s) 179
BsuI GTATCC 1 cut(s) 531
BsuRI GGCC 7 cut(s) 689, 784, 1258, 1382, 1492, 1709, 2716
BtgI CCRYGG 1 cut(s) 1143
BtsCI GGATG 2 cut(s) 373, 1383
BtsIMutI CAGTG 4 cut(s) 135, 697, 2363, 2574
BveI ACCTGC 2 cut(s) 748, 844
Cac8I GCNNGC 2 cut(s) 1707, 2699
CciI TCATGA 3 cut(s) 459, 1657, 2665
CfoI GCGC 1 cut(s) 1705
Csp6I GTAC 7 cut(s) 354, 881, 1373, 1558, 1688, 2241, 2318
CspCI CAANNNNNGTGG 2 cut(s) 2171, 2206
CviQI GTAC 7 cut(s) 354, 881, 1373, 1558, 1688, 2241, 2318
DdeI CTNAG 8 cut(s) 374, 474, 531, 1291, 2355, 2550, 2592, 2705
DrdI GACNNNNNNGTC 1 cut(s) 1039
DseDI GACNNNNNNGTC 1 cut(s) 1039
EaeI YGGCCR 1 cut(s) 782
EciI GGCGGA 1 cut(s) 1650
Ecl136II GAGCTC 1 cut(s) 2354
Eco130I CCWWGG 4 cut(s) 1098, 1253, 1740, 2384
Eco147I AGGCCT 1 cut(s) 2716
Eco24I GRGCYC 1 cut(s) 2356
Eco31I GGTCTC 1 cut(s) 2101
Eco53kI GAGCTC 1 cut(s) 2354
Eco57I CTGAAG 2 cut(s) 21, 1203
Eco72I CACGTG 1 cut(s) 719
Eco88I CYCGRG 2 cut(s) 130, 2543
EcoICRI GAGCTC 1 cut(s) 2354
EcoNI CCTNNNNNAGG 1 cut(s) 682
EcoO109I RGGNCCY 4 cut(s) 61, 675, 2375, 2510
EcoT14I CCWWGG 4 cut(s) 1098, 1253, 1740, 2384
EcoT22I ATGCAT 1 cut(s) 831
EcoT38I GRGCYC 1 cut(s) 2356
ErhI CCWWGG 4 cut(s) 1098, 1253, 1740, 2384
Esp3I CGTCTC 1 cut(s) 1567
FaqI GGGAC 1 cut(s) 2715
FauNDI CATATG 3 cut(s) 103, 1212, 1392
FbaI TGATCA 3 cut(s) 886, 1207, 1266
FblI GTMKAC 2 cut(s) 178, 1043
Fnu4HI GCNGC 6 cut(s) 150, 153, 450, 1095, 1259, 2695
FokI GGATG 2 cut(s) 380, 1390
FriOI GRGCYC 1 cut(s) 2356
Fsp4HI GCNGC 6 cut(s) 150, 153, 450, 1095, 1259, 2695
FspBI CTAG 8 cut(s) 570, 941, 1523, 1836, 1845, 1851, 2445, 2712
GlaI GCGC 1 cut(s) 1704
GluI GCNGC 6 cut(s) 150, 153, 450, 1095, 1259, 2695
GsaI CCCAGC 1 cut(s) 795
GsuI CTGGAG 1 cut(s) 1741
HaeIII GGCC 7 cut(s) 689, 784, 1258, 1382, 1492, 1709, 2716
HhaI GCGC 1 cut(s) 1705
Hin1I GRCGYC 1 cut(s) 1662
Hin6I GCGC 1 cut(s) 1703
HinP1I GCGC 1 cut(s) 1703
HincII GTYRAC 3 cut(s) 1044, 1357, 2200
HindII GTYRAC 3 cut(s) 1044, 1357, 2200
HindIII AAGCTT 2 cut(s) 1271, 1404
HphI GGTGA 8 cut(s) 203, 299, 571, 917, 1310, 1436, 2021, 2233
Hpy166II GTNNAC 7 cut(s) 179, 338, 881, 928, 1044, 1357, 2200
Hpy8I GTNNAC 7 cut(s) 179, 338, 881, 928, 1044, 1357, 2200
Hpy99I CGWCG 4 cut(s) 1036, 1246, 1578, 1896
HpyAV CCTTC 8 cut(s) 74, 305, 1503, 1720, 2086, 2188, 2664, 2684
HpyCH4III ACNGT 3 cut(s) 139, 358, 2578
HpyCH4IV ACGT 7 cut(s) 718, 962, 1560, 1618, 1654, 1662, 2468
HpyF10VI GCNNNNNNNGC 5 cut(s) 95, 400, 518, 661, 1070
HpyF3I CTNAG 8 cut(s) 374, 474, 531, 1291, 2355, 2550, 2592, 2705
HpySE526I ACGT 7 cut(s) 718, 962, 1560, 1618, 1654, 1662, 2468
Hsp92I GRCGYC 1 cut(s) 1662
HspAI GCGC 1 cut(s) 1703
Ksp22I TGATCA 3 cut(s) 886, 1207, 1266
LmnI GCTCC 5 cut(s) 629, 652, 1144, 2074, 2351
Lsp1109I GCAGC 5 cut(s) 161, 164, 461, 1106, 2706
LweI GCATC 5 cut(s) 358, 412, 641, 1351, 1443
MaeI CTAG 8 cut(s) 570, 941, 1523, 1836, 1845, 1851, 2445, 2712
MaeII ACGT 7 cut(s) 718, 962, 1560, 1618, 1654, 1662, 2468
MaeIII GTNAC 5 cut(s) 79, 641, 802, 905, 1650
MfeI CAATTG 5 cut(s) 1529, 1698, 1715, 2183, 2324
MflI RGATCY 3 cut(s) 244, 700, 2569
MhlI GDGCHC 4 cut(s) 137, 340, 1141, 2356
MlyI GAGTC 4 cut(s) 812, 1091, 2195, 2542
MmeI TCCRAC 5 cut(s) 607, 782, 1176, 1874, 2115
Mph1103I ATGCAT 1 cut(s) 831
MroXI GAANNNNTTC 3 cut(s) 549, 1159, 1881
MseI TTAA 9 cut(s) 20, 161, 210, 347, 824, 993, 2105, 2274, 2363
MslI CAYNNNNRTG 4 cut(s) 140, 189, 717, 1947
MspA1I CMGCKG 1 cut(s) 1064
MunI CAATTG 5 cut(s) 1529, 1698, 1715, 2183, 2324
MvnI CGCG 1 cut(s) 1222
MwoI GCNNNNNNNGC 5 cut(s) 95, 400, 518, 661, 1070
NdeI CATATG 3 cut(s) 103, 1212, 1392
NlaIV GGNNCC 5 cut(s) 126, 1218, 1546, 2070, 2731
NmuCI GTSAC 2 cut(s) 79, 905
NsiI ATGCAT 1 cut(s) 831
NspI RCATGY 2 cut(s) 73, 1946
NspV TTCGAA 1 cut(s) 1747
PagI TCATGA 3 cut(s) 459, 1657, 2665
PceI AGGCCT 1 cut(s) 2716
PcsI WCGNNNNNNNCGW 2 cut(s) 1040, 1132
PdmI GAANNNNTTC 3 cut(s) 549, 1159, 1881
PfeI GAWTC 8 cut(s) 194, 865, 1238, 1441, 1585, 2138, 2284, 2676
PflMI CCANNNNNTGG 3 cut(s) 262, 791, 2032
PkrI GCNGC 6 cut(s) 151, 154, 451, 1096, 1260, 2696
Ple19I CGATCG 1 cut(s) 1038
PleI GAGTC 4 cut(s) 812, 1090, 2195, 2542
PmaCI CACGTG 1 cut(s) 719
PmlI CACGTG 1 cut(s) 719
PpsI GAGTC 4 cut(s) 812, 1090, 2195, 2542
Ppu21I YACGTR 3 cut(s) 719, 963, 1619
PpuMI RGGWCCY 4 cut(s) 61, 675, 2375, 2510
PshBI ATTAAT 3 cut(s) 210, 347, 2105
Psp124BI GAGCTC 1 cut(s) 2356
Psp5II RGGWCCY 4 cut(s) 61, 675, 2375, 2510
PspCI CACGTG 1 cut(s) 719
PspFI CCCAGC 1 cut(s) 791
PspN4I GGNNCC 5 cut(s) 126, 1218, 1546, 2070, 2731
PspPPI RGGWCCY 4 cut(s) 61, 675, 2375, 2510
PsrI GAACNNNNNNTAC 2 cut(s) 2431, 2463
PstI CTGCAG 1 cut(s) 844
PsuI RGATCY 3 cut(s) 244, 700, 2569
PvuI CGATCG 1 cut(s) 1038
RsaI GTAC 7 cut(s) 355, 882, 1374, 1559, 1689, 2242, 2319
RsaNI GTAC 7 cut(s) 354, 881, 1373, 1558, 1688, 2241, 2318
RseI CAYNNNNRTG 4 cut(s) 140, 189, 717, 1947
SacI GAGCTC 1 cut(s) 2356
SalI GTCGAC 1 cut(s) 1042
SaqAI TTAA 9 cut(s) 20, 161, 210, 347, 824, 993, 2105, 2274, 2363
SatI GCNGC 6 cut(s) 150, 153, 450, 1095, 1259, 2695
SchI GAGTC 4 cut(s) 812, 1091, 2195, 2542
SduI GDGCHC 4 cut(s) 137, 340, 1141, 2356
SfaNI GCATC 5 cut(s) 358, 412, 641, 1351, 1443
SfcI CTRYAG 4 cut(s) 84, 222, 704, 840
SfuI TTCGAA 1 cut(s) 1747
SmiMI CAYNNNNRTG 4 cut(s) 140, 189, 717, 1947
SmlI CTYRAG 4 cut(s) 56, 434, 1274, 2505
SmoI CTYRAG 4 cut(s) 56, 434, 1274, 2505
SseBI AGGCCT 1 cut(s) 2716
SsiI CCGC 4 cut(s) 1062, 1220, 1259, 1635
SspI AATATT 1 cut(s) 345
SspMI CTAG 8 cut(s) 570, 941, 1523, 1836, 1845, 1851, 2445, 2712
SstI GAGCTC 1 cut(s) 2356
StuI AGGCCT 1 cut(s) 2716
StyI CCWWGG 4 cut(s) 1098, 1253, 1740, 2384
TaaI ACNGT 3 cut(s) 139, 358, 2578
TaiI ACGT 7 cut(s) 721, 965, 1563, 1621, 1657, 1665, 2471
TatI WGTACW 4 cut(s) 353, 880, 1687, 2317
TauI GCSGC 1 cut(s) 1261
TfiI GAWTC 8 cut(s) 194, 865, 1238, 1441, 1585, 2138, 2284, 2676
Tru1I TTAA 9 cut(s) 20, 161, 210, 347, 824, 993, 2105, 2274, 2363
Tru9I TTAA 9 cut(s) 20, 161, 210, 347, 824, 993, 2105, 2274, 2363
TscAI CASTG 4 cut(s) 142, 697, 2363, 2581
TseFI GTSAC 2 cut(s) 79, 905
TseI GCWGC 5 cut(s) 149, 152, 449, 1094, 2694
Tsp45I GTSAC 2 cut(s) 79, 905
TspGWI ACGGA 4 cut(s) 1015, 1220, 1390, 1610
TspRI CASTG 4 cut(s) 142, 697, 2363, 2581
Van91I CCANNNNNTGG 3 cut(s) 262, 791, 2032
VneI GTGCAC 1 cut(s) 336
VspI ATTAAT 3 cut(s) 210, 347, 2105
XagI CCTNNNNNAGG 1 cut(s) 682
XapI RAATTY 8 cut(s) 1054, 1103, 1156, 1484, 1499, 1750, 1762, 2411
XceI RCATGY 2 cut(s) 73, 1946
XmiI GTMKAC 2 cut(s) 178, 1043
XmnI GAANNNNTTC 3 cut(s) 549, 1159, 1881
XspI CTAG 8 cut(s) 570, 941, 1523, 1836, 1845, 1851, 2445, 2712
ZraI GACGTC 1 cut(s) 1663
Zsp2I ATGCAT 1 cut(s) 831
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.