RchiOBHm_Chr7g0227361

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0020126
Physical Location & Seq
Reverse (-)
50617848 .. 50620691
2844 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20363

Sequence Viewer

Length: 1902 bp
ATGAGATTATTAGCCGCTGGAATATTCAAGAAGATTTGGTTTTGGACCTTTCGAGTTACTCGTACAAAAAGTCAGGGTTTCATTGTTAGTCGCCCTAGGGTTTCTTTCCCATATATAAGCAACCTTAAACGGCTGCAGAACTATCTTTTATCATATTATCAATCAAAATTGAGAGTTTTCTCTTTTATCTCTGGTGGACTCCAGAATCTTTTGCTTAGGTTTATTGCTAGTAAACCTAGTTATCAATCCGTCTGCTTCAGGTGGTATCAGAGCAGGTCTTCCCTGTCTCTTTTATTTACCTTGGTAGCAATGGGTGAAGTTACAAAAGCAGATATTGAAGCTCTTACAGCAGCGTTTACCGCTGCCATCAACTCCATGAACAATCAGATTGGAGAAATTTGTGGATTGTTGGGAGAGAGGAACAATAACAACAACAACAACAACAACAATCAGAATAGAGGCGGGGAAGGAGGCCAGAGGGCTAGAGCTCCACGTGGTGGTGGAGGTGATATTAATTATGATTCCGAGGTTCTCATAACCAAGGCTGATATTCCTTACTTTTCTGGTCACATGAGCGCGGAGGATTTTCTGGATTGGCAGGTTGAAGTGGATAGATTCTTTAAGACCATGGAGGTTCCAAAACACAAGCAGGCTAAGATGGTTTCTCAGAAGCTAAAGAAAGATGCTGCTTATTGGTGGGATCAGTTGCAAAGCTCTCGTCAGAGGCAAGGCAAAGAGCGTGTTCGGACATGGCGCAAGATGAAAGGTCTTCTTAGCGAAAAATTTATGCCAAGAGATTTTTCAATCAAGAATTATTCTCCTAAGGTTTTCGAGAAGAAGTTAGAGTTCAAACTTTCTTTGCCTGTTGAGATTAATAGTTTATCTGAAGAAAAACCAGAAGAGTTTCTAATGGAAGGAGCTCCACAAGAAATCATCCATGATAACCAAGAAGAGGTTAAAGATGAAATTGTTCATGTAGACATGGTTATTAATAATGAGGAAGTCTTGGAGCCTGAAATAAATCACTCAGAACCAGATATCATTCAAGAATGCAACCTTGAGAGCCGAGACAAATCTACGAAGGTTGCGTATGAGAGCCAAAATGCATATGACAGACTCATCTTTGGCGTTGGGTTCATGATTGTGCCATCAAAATTTGCAGAGGATCAGGCCAACAATCCACAGTTATCAATCCGTCTGCGTCAAAAAAGATATGGGATAGCGCTTGGAATGGCAAGGGGGCTTGCTTACTTGCACAGTGGATGCATTCCCAAGATAGTCCACTGTGACATCAAACCAGAATACATACTTCTGCATGATGATTTACAAGTGAAGATATCAGACTTTGGGGTTTCCAAGTTTATAAGTTACGAAAAATCCAAACTGTTGACGACACTTAGAGAAACTAGGGGATATCTTGCTCCGGAATGGCTTACTAGCTATGGCATCAGTGAAAAAATTGATGTATACAGTTATGGGATGGTGTTGCTAGAATTGGTGAGGGGAAGAAGAAATTGCTTGTTTCAAAGTAGTGGCACCAGAAATGATGACACTGATGGAAATGAAAAATGTTTCAATTCAATCAGTTCGGAAGAAAGAATGGTGTACTTCCCACAACTTGCACTCCAAATGCATAAGAAAATGAGGTATATGGAACTAGCTGACCCAAGGTTAGAGGGTTGTGTGAGGAGTGAGGAGGTTGAGAAGCTAGTGAGGGTAGCCTTGTGTTGTGTGCATGTAGGGCCAACACTGAGGCCAAGTATGACCAATGTGGTTGCTATGTTGGAAGGCAGATTACCTATAGCTGAGCCTAGCATTGAAGCATTAGAATTCTTGCAGTCCTATGGTGGAAATGTCTCAGAGGCATCCAGCAAGCTAACAAAAGTGGGATGTGCAGAATAA

Protein Analysis

633

Amino Acids

72.37

Weight (kDa)

8.54

Isoelectric Point (pI)

44.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 403 - 537 5.8e-17 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 407 - 593 7.4e-28 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020126)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g23981 FvH4_4g10392
malus_domestica MD14G1073100.v1.1
pyrus_communis pycom01g01230
rosa_chinensis RchiOBHm_Chr7g0227361
rosa_roxburghii Rroxscaffold_6G00402460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1364
Acc36I ACCTGC 2 cut(s) 264, 589
AccB1I GGYRCC 1 cut(s) 1535
AccB7I CCANNNNNTGG 1 cut(s) 497
AccI GTMKAC 2 cut(s) 978, 1467
AccII CGCG 1 cut(s) 578
AccIII TCCGGA 1 cut(s) 1423
AciI CCGC 4 cut(s) 15, 360, 462, 578
AclWI GGATC 2 cut(s) 708, 1173
AcsI RAATTY 4 cut(s) 396, 782, 1154, 1829
AcuI CTGAAG 2 cut(s) 241, 906
AcvI CACGTG 1 cut(s) 494
AdeI CACNNNGTG 1 cut(s) 497
AfaI GTAC 2 cut(s) 64, 1607
AfeI AGCGCT 1 cut(s) 1224
AfiI CCNNNNNNNGG 2 cut(s) 497, 952
AjuI GAANNNNNNNTTGG 2 cut(s) 784, 816
Alw21I GWGCWC 2 cut(s) 490, 922
Alw26I GTCTC 3 cut(s) 291, 1062, 1861
AlwI GGATC 2 cut(s) 708, 1173
Aor13HI TCCGGA 1 cut(s) 1423
Aor51HI AGCGCT 1 cut(s) 1224
AoxI GGCC 4 cut(s) 472, 1170, 1742, 1754
ApeKI GCWGC 4 cut(s) 133, 350, 362, 686
ApoI RAATTY 4 cut(s) 396, 782, 1154, 1829
ArsI GACNNNNNNTTYG 2 cut(s) 703, 735
AseI ATTAAT 3 cut(s) 513, 873, 990
Asp700I GAANNNNTTC 2 cut(s) 903, 969
AspA2I CCTAGG 1 cut(s) 95
AspLEI GCGC 3 cut(s) 578, 756, 1225
AspS9I GGNCC 2 cut(s) 45, 1742
AsuHPI GGTGA 3 cut(s) 326, 518, 1510
AvaII GGWCC 1 cut(s) 45
AvrII CCTAGG 1 cut(s) 95
AxyI CCTNAGG 1 cut(s) 822
BanI GGYRCC 1 cut(s) 1535
BanII GRGCYC 2 cut(s) 490, 922
BarI GAAGNNNNNNTAC 2 cut(s) 1779, 1811
BbrPI CACGTG 1 cut(s) 494
BbsI GAAGAC 2 cut(s) 270, 761
Bbv12I GWGCWC 2 cut(s) 490, 922
BbvI GCAGC 4 cut(s) 120, 349, 362, 673
BccI CCATC 5 cut(s) 374, 652, 1156, 1474, 1550
BceAI ACGGC 1 cut(s) 146
BcgI CGANNNNNNTGC 2 cut(s) 698, 732
BcoDI GTCTC 3 cut(s) 291, 1062, 1861
BfmI CTRYAG 2 cut(s) 134, 1800
BfoI RGCGCY 1 cut(s) 1226
BfuAI ACCTGC 2 cut(s) 264, 589
BisI GCNGC 5 cut(s) 15, 134, 351, 363, 687
BlnI CCTAGG 1 cut(s) 95
BlpI GCTNAGC 1 cut(s) 1806
BlsI GCNGC 5 cut(s) 16, 135, 352, 364, 688
Bme18I GGWCC 1 cut(s) 45
BmgT120I GGNCC 2 cut(s) 45, 1742
BmiI GGNNCC 3 cut(s) 636, 1011, 1537
BmsI GCATC 4 cut(s) 673, 1253, 1455, 1874
BpiI GAAGAC 2 cut(s) 270, 761
BpmI CTGGAG 1 cut(s) 185
Bpu10I CCTNAGC 1 cut(s) 215
Bpu1102I GCTNAGC 1 cut(s) 1806
BpuEI CTTGAG 1 cut(s) 1079
BsaAI YACGTR 1 cut(s) 494
BsaJI CCNNGG 6 cut(s) 95, 300, 525, 540, 627, 1667
BsaWI WCCGGW 1 cut(s) 1423
BsaXI ACNNNNNCTCC 2 cut(s) 1608, 1638
Bsc4I CCNNNNNNNGG 2 cut(s) 497, 952
Bse21I CCTNAGG 1 cut(s) 822
Bse3DI GCAATG 1 cut(s) 315
BseAI TCCGGA 1 cut(s) 1423
BseDI CCNNGG 6 cut(s) 95, 300, 525, 540, 627, 1667
BseGI GGATG 5 cut(s) 933, 1268, 1485, 1865, 1895
BseLI CCNNNNNNNGG 2 cut(s) 497, 952
BseMI GCAATG 1 cut(s) 315
BseMII CTCAG 5 cut(s) 680, 1041, 1742, 1797, 1872
BseRI GAGGAG 2 cut(s) 1702, 1709
BseXI GCAGC 4 cut(s) 120, 349, 362, 673
Bsh1236I CGCG 1 cut(s) 578
BshFI GGCC 4 cut(s) 474, 1172, 1744, 1756
BshNI GGYRCC 1 cut(s) 1535
BsiHKAI GWGCWC 2 cut(s) 490, 922
BsiSI CCGG 1 cut(s) 1424
BslI CCNNNNNNNGG 2 cut(s) 497, 952
BsmAI GTCTC 3 cut(s) 291, 1062, 1861
BsmI GAATGC 2 cut(s) 1055, 1266
BsnI GGCC 4 cut(s) 474, 1172, 1744, 1756
Bsp1286I GDGCHC 2 cut(s) 490, 922
Bsp13I TCCGGA 1 cut(s) 1423
Bsp143I GATC 2 cut(s) 700, 1165
Bsp1720I GCTNAGC 1 cut(s) 1806
Bsp19I CCATGG 1 cut(s) 627
BspACI CCGC 4 cut(s) 15, 360, 462, 578
BspANI GGCC 4 cut(s) 474, 1172, 1744, 1756
BspCNI CTCAG 5 cut(s) 679, 1040, 1743, 1798, 1871
BspEI TCCGGA 1 cut(s) 1423
BspFNI CGCG 1 cut(s) 578
BspHI TCATGA 1 cut(s) 1137
BspLI GGNNCC 3 cut(s) 636, 1011, 1537
BspMAI CTGCAG 1 cut(s) 138
BspMI ACCTGC 2 cut(s) 264, 589
BspPI GGATC 2 cut(s) 708, 1173
BspT107I GGYRCC 1 cut(s) 1535
BsrDI GCAATG 1 cut(s) 315
BssECI CCNNGG 6 cut(s) 95, 300, 525, 540, 627, 1667
BssMI GATC 2 cut(s) 700, 1165
BssNAI GTATAC 1 cut(s) 1468
BssT1I CCWWGG 5 cut(s) 95, 300, 540, 627, 1667
Bst1107I GTATAC 1 cut(s) 1468
Bst4CI ACNGT 5 cut(s) 1185, 1259, 1286, 1386, 1472
Bst6I CTCTTC 2 cut(s) 894, 945
BstBAI YACGTR 1 cut(s) 494
BstC8I GCNNGC 3 cut(s) 651, 1245, 1874
BstDSI CCRYGG 1 cut(s) 627
BstF5I GGATG 5 cut(s) 933, 1268, 1485, 1865, 1895
BstFNI CGCG 1 cut(s) 578
BstH2I RGCGCY 1 cut(s) 1226
BstHHI GCGC 3 cut(s) 578, 756, 1225
BstKTI GATC 2 cut(s) 703, 1168
BstMAI GTCTC 3 cut(s) 291, 1062, 1861
BstMBI GATC 2 cut(s) 700, 1165
BstMWI GCNNNNNNNGC 3 cut(s) 347, 359, 1741
BstNSI RCATGY 1 cut(s) 1739
BstSFI CTRYAG 2 cut(s) 134, 1800
BstUI CGCG 1 cut(s) 578
BstV1I GCAGC 4 cut(s) 120, 349, 362, 673
BstV2I GAAGAC 2 cut(s) 270, 761
BstZ17I GTATAC 1 cut(s) 1468
Bsu36I CCTNAGG 1 cut(s) 822
BsuRI GGCC 4 cut(s) 474, 1172, 1744, 1756
BtgI CCRYGG 1 cut(s) 627
BtsCI GGATG 5 cut(s) 933, 1268, 1485, 1865, 1895
BtsIMutI CAGTG 5 cut(s) 1264, 1282, 1456, 1551, 1748
BveI ACCTGC 2 cut(s) 264, 589
Cac8I GCNNGC 3 cut(s) 651, 1245, 1874
CciI TCATGA 1 cut(s) 1137
CfoI GCGC 3 cut(s) 578, 756, 1225
Cfr13I GGNCC 2 cut(s) 45, 1742
CseI GACGC 1 cut(s) 1190
Csp6I GTAC 2 cut(s) 63, 1606
CviQI GTAC 2 cut(s) 63, 1606
DpnI GATC 2 cut(s) 702, 1167
DpnII GATC 2 cut(s) 700, 1165
DraIII CACNNNGTG 1 cut(s) 497
Eam1104I CTCTTC 2 cut(s) 894, 945
EarI CTCTTC 2 cut(s) 894, 945
Ecl136II GAGCTC 2 cut(s) 488, 920
Eco130I CCWWGG 5 cut(s) 95, 300, 540, 627, 1667
Eco24I GRGCYC 2 cut(s) 490, 922
Eco32I GATATC 3 cut(s) 1039, 1338, 1415
Eco47I GGWCC 1 cut(s) 45
Eco47III AGCGCT 1 cut(s) 1224
Eco53kI GAGCTC 2 cut(s) 488, 920
Eco57I CTGAAG 2 cut(s) 241, 906
Eco72I CACGTG 1 cut(s) 494
Eco81I CCTNAGG 1 cut(s) 822
EcoICRI GAGCTC 2 cut(s) 488, 920
EcoRI GAATTC 1 cut(s) 1829
EcoRV GATATC 3 cut(s) 1039, 1338, 1415
EcoT14I CCWWGG 5 cut(s) 95, 300, 540, 627, 1667
EcoT22I ATGCAT 3 cut(s) 1108, 1268, 1635
EcoT38I GRGCYC 2 cut(s) 490, 922
ErhI CCWWGG 5 cut(s) 95, 300, 540, 627, 1667
FalI AAGNNNNNCTT 2 cut(s) 756, 788
FauI CCCGC 1 cut(s) 455
FauNDI CATATG 1 cut(s) 1108
FblI GTMKAC 2 cut(s) 978, 1467
Fnu4HI GCNGC 5 cut(s) 15, 134, 351, 363, 687
FokI GGATG 4 cut(s) 920, 1275, 1492, 1852
FriOI GRGCYC 2 cut(s) 490, 922
Fsp4HI GCNGC 5 cut(s) 15, 134, 351, 363, 687
GlaI GCGC 3 cut(s) 577, 755, 1224
GluI GCNGC 5 cut(s) 15, 134, 351, 363, 687
GsuI CTGGAG 1 cut(s) 185
HaeII RGCGCY 1 cut(s) 1226
HaeIII GGCC 4 cut(s) 474, 1172, 1744, 1756
HapII CCGG 1 cut(s) 1424
HgaI GACGC 1 cut(s) 1190
HhaI GCGC 3 cut(s) 578, 756, 1225
Hin6I GCGC 3 cut(s) 576, 754, 1223
HinP1I GCGC 3 cut(s) 576, 754, 1223
HincII GTYRAC 1 cut(s) 1389
HindII GTYRAC 1 cut(s) 1389
HinfI GANTC 5 cut(s) 198, 205, 521, 615, 1116
HpaII CCGG 1 cut(s) 1424
HphI GGTGA 3 cut(s) 326, 518, 1510
Hpy166II GTNNAC 8 cut(s) 197, 233, 357, 979, 1282, 1389, 1468, 1606
Hpy188III TCNNGA 8 cut(s) 28, 202, 590, 808, 832, 1046, 1138, 1424
Hpy8I GTNNAC 8 cut(s) 197, 233, 357, 979, 1282, 1389, 1468, 1606
HpyAV CCTTC 4 cut(s) 461, 908, 1075, 1781
HpyCH4III ACNGT 5 cut(s) 1185, 1259, 1286, 1386, 1472
HpyCH4IV ACGT 1 cut(s) 493
HpyF10VI GCNNNNNNNGC 3 cut(s) 347, 359, 1741
HpySE526I ACGT 1 cut(s) 493
HspAI GCGC 3 cut(s) 576, 754, 1223
Kpn2I TCCGGA 1 cut(s) 1423
Kzo9I GATC 2 cut(s) 700, 1165
LmnI GCTCC 5 cut(s) 493, 917, 925, 1009, 1426
Lsp1109I GCAGC 4 cut(s) 120, 349, 362, 673
LweI GCATC 4 cut(s) 673, 1253, 1455, 1874
MaeII ACGT 1 cut(s) 493
MaeIII GTNAC 5 cut(s) 55, 319, 566, 1286, 1367
MalI GATC 2 cut(s) 702, 1167
MboI GATC 2 cut(s) 700, 1165
MhlI GDGCHC 2 cut(s) 490, 922
MlyI GAGTC 2 cut(s) 192, 1110
MmeI TCCRAC 1 cut(s) 1764
Mph1103I ATGCAT 3 cut(s) 1108, 1268, 1635
MroI TCCGGA 1 cut(s) 1423
MroXI GAANNNNTTC 2 cut(s) 903, 969
MseI TTAA 6 cut(s) 126, 513, 621, 873, 957, 990
MslI CAYNNNNRTG 1 cut(s) 1142
MspA1I CMGCKG 2 cut(s) 17, 362
MspI CCGG 1 cut(s) 1424
Mva1269I GAATGC 2 cut(s) 1055, 1266
MvnI CGCG 1 cut(s) 578
MwoI GCNNNNNNNGC 3 cut(s) 347, 359, 1741
NcoI CCATGG 1 cut(s) 627
NdeI CATATG 1 cut(s) 1108
NdeII GATC 2 cut(s) 700, 1165
NlaIV GGNNCC 3 cut(s) 636, 1011, 1537
NmeAIII GCCGAG 1 cut(s) 1091
NmuCI GTSAC 2 cut(s) 566, 1286
NsiI ATGCAT 3 cut(s) 1108, 1268, 1635
NspI RCATGY 1 cut(s) 1739
PagI TCATGA 1 cut(s) 1137
PcsI WCGNNNNNNNCGW 2 cut(s) 58, 1085
PctI GAATGC 2 cut(s) 1055, 1266
PdmI GAANNNNTTC 2 cut(s) 903, 969
PfeI GAWTC 3 cut(s) 205, 521, 615
PflMI CCANNNNNTGG 1 cut(s) 497
PkrI GCNGC 5 cut(s) 16, 135, 352, 364, 688
PleI GAGTC 2 cut(s) 192, 1110
PmaCI CACGTG 1 cut(s) 494
PmlI CACGTG 1 cut(s) 494
PpsI GAGTC 2 cut(s) 192, 1110
Ppu21I YACGTR 1 cut(s) 494
PshBI ATTAAT 3 cut(s) 513, 873, 990
PsiI TTATAA 1 cut(s) 1364
Psp124BI GAGCTC 2 cut(s) 490, 922
PspCI CACGTG 1 cut(s) 494
PspN4I GGNNCC 3 cut(s) 636, 1011, 1537
PspPI GGNCC 2 cut(s) 45, 1742
PstI CTGCAG 1 cut(s) 138
RsaI GTAC 2 cut(s) 64, 1607
RsaNI GTAC 2 cut(s) 63, 1606
RseI CAYNNNNRTG 1 cut(s) 1142
SacI GAGCTC 2 cut(s) 490, 922
SaqAI TTAA 6 cut(s) 126, 513, 621, 873, 957, 990
SatI GCNGC 5 cut(s) 15, 134, 351, 363, 687
Sau3AI GATC 2 cut(s) 700, 1165
Sau96I GGNCC 2 cut(s) 45, 1742
SchI GAGTC 2 cut(s) 192, 1110
SduI GDGCHC 2 cut(s) 490, 922
SfaNI GCATC 4 cut(s) 673, 1253, 1455, 1874
SfcI CTRYAG 2 cut(s) 134, 1800
SinI GGWCC 1 cut(s) 45
SmiMI CAYNNNNRTG 1 cut(s) 1142
SmlI CTYRAG 1 cut(s) 1058
SmoI CTYRAG 1 cut(s) 1058
SsiI CCGC 4 cut(s) 15, 360, 462, 578
SspI AATATT 1 cut(s) 24
SstI GAGCTC 2 cut(s) 490, 922
StyI CCWWGG 5 cut(s) 95, 300, 540, 627, 1667
TaaI ACNGT 5 cut(s) 1185, 1259, 1286, 1386, 1472
TaiI ACGT 1 cut(s) 496
TaqI TCGA 2 cut(s) 52, 831
TatI WGTACW 1 cut(s) 1605
TauI GCSGC 1 cut(s) 17
TfiI GAWTC 3 cut(s) 205, 521, 615
Tru1I TTAA 6 cut(s) 126, 513, 621, 873, 957, 990
Tru9I TTAA 6 cut(s) 126, 513, 621, 873, 957, 990
TscAI CASTG 5 cut(s) 1264, 1289, 1456, 1558, 1755
TseFI GTSAC 2 cut(s) 566, 1286
TseI GCWGC 4 cut(s) 133, 350, 362, 686
Tsp45I GTSAC 2 cut(s) 566, 1286
TspDTI ATGAA 7 cut(s) 70, 392, 776, 962, 978, 1126, 1578
TspGWI ACGGA 2 cut(s) 238, 1184
TspRI CASTG 5 cut(s) 1264, 1289, 1456, 1558, 1755
Van91I CCANNNNNTGG 1 cut(s) 497
VpaK11BI GGWCC 1 cut(s) 45
VspI ATTAAT 3 cut(s) 513, 873, 990
XapI RAATTY 4 cut(s) 396, 782, 1154, 1829
XceI RCATGY 1 cut(s) 1739
XmaJI CCTAGG 1 cut(s) 95
XmiI GTMKAC 2 cut(s) 978, 1467
XmnI GAANNNNTTC 2 cut(s) 903, 969
Zsp2I ATGCAT 3 cut(s) 1108, 1268, 1635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.