Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0007519

Physical Location & Seq

Genomic Coordinates Forward (+)

11539908 .. 11544551

Gene Structure 4644 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1090500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2610 bp

ATGACGACCCTCAAAATCTCCAAAAAACACCACAAGCATTTGAACAATCCATTCCCTTCCCCACCCACATCCCTCCCTCTCCTCCAAGGCAACCTCATCTTCAATTCCCAAACTCTCCGCCCTTCTTCGCCTCACCATTTCCCACTCGGCAACCATTTCCACCTCTCTTGGACCTCCACCGATGGCGGCTCTCTCTCCATTCACCACCGATCCCACCCCAAGAGAGCCCTCTGGTCCACCCTCCCCGGCCACCCTTTTGTTTCTGCAGCATTGGCCGAAACTGAGGTGGAAGAAAGCAGGGGCTCCTTCCTTGTCAGAGACACAAAGCTTCATCTGGTTTGCAATCACCAAACCATTCAGGAAATAAGGGTGATCAACAATCACGATCAGCTTCCAGATTCTCCATCTGGGTTTTTGGGATTCGATCGGAAACTGGATTCCCCAGTTACCCAATTCCAATTCCCGATCGTTCTTGTAACTGGCTGGGTTTTCAATACCAAAACAAGGACGAGGATTTCTCAGAGGGAATGTGAGGGGAAGGGGCCTTCTACTTGTGCAAAGTATTGGGTTTTCTTTGAGCAGAAAAACAGTGATCAGATTGGATTCCGGGTGAAGGTGGGACAGCCCAACTTTGAATTGAGCACAAAAGCTTGTCCAAGTAGTGGTGGTTCAGGAAGATACAAGGGTTTTCGGAGGAGGCTAGGCCGGTTTCAGAAGCGCCGGCTGCGGTGGTTCTGGTCATTTGCAAGGCCAAGAGGGTTTGTCCTGGTTTCCTCATCAGAGGAAGAGTTGGAGGAACTCAAGGCTCAAGAGTTCAAGGAATTCAACAGGGTTTGCTTGACATATTCGAGTGACGAAAATGAGAGGTTTTTCGGTTTTGGGGAGCAGTTTTCTCATATGGATTTTAAGGGGAAGAGGGTTCCCATTTTGGTCCAGGAACAAGGTATTGGGAGAGGTGATCAACCTATTACTTTTGCAGCTAACTTGATCAGCTATAGGGCTGGTGGCGATTGGAGTACAACGTACGCTCCTTCTCCATTCTATATGACTTCCAAGATGAGGTCTCTTTACCTCGAAGGATATGAATATTCCGTGTTTGATCTAACGAAGCACGATAGAGTTCAGATTCAGATACATGGAAACTCAGTTGAAGGAAGGATATTGCACGGGACCTCACCATCTGAGCTCATTGAATGTTTCACTGAAACCATTGGAAGACCTCCCAAACTTCCCGACTGGATTATTTCCGGAGCTGTTGTTGGCATGCAGGGCGGTACAGAATCAGCTCGCCATATTTGGAATGAGCTCAAGACTTATGATGCTCCCATTTCAGCATTTTGGCTGCAGGATTGGGTGGGCCAGAGAGAGACGTTGGTTGGATCACAACTATGGTGGAATTGGGAAGTAGATTCAACAAGGTATAAGGGATGGAAAGAACTGGTTAAAGATCTTAGTGCTCAGCATATTAGAGTGATGACTTACTGCAACCCATGTCTAGCTCCGTGTCATGAGAAGCCAAACAGAAGGAGAAATCTCTTTGAGGAAGCAAAGAAGTTGGACATATTAGTGAAAGACAGGCATGGAGAAACGTACATGGTTCCAAATACAGCTTTTGATGTAGGAATGTTGGATTTGACACATCCCGATACTGGAAGTTGGTTCAAGCAGATTCTTCGAGCAATGGTGGATGATGGAGTCAGAGGATGGATGGCTGATTTTGGTGAAGGTCTTCCTGTGGATGCAACCCTCTATTCAGGTGAAGATCCTATTTCAGCCCATAACAAATACCCAGAGCTGTGGGCAAAAATTAACAGAGAGTTTGTGGATGAATGGAAAGCTATCCGTTCGAATGAGGAGAATGAAGACCCCGAAGATGCATTGGTCTTTTTTATGAGGGCGGGTTACAGAGATAGTCCCAAATGGGGGATGCTATTTTGGGAAGGAGACCAAATGGTAAGTTGGCAGACTAATGATGGAATAAAGAGTGCTGTTGTTGGCCTACTCAGTAGTGGACTTTCTGGGTATGCATTTAATCACAGTGACATTGGAGGGTACTGTGCTGTAAACTTACCTTTCATTAACTATCGAAGAAGTGAAGAGTTGCTTTTGCGATGGATGGAGCTAAATGCTTTCACGACTGTTTTCCGGACGCATGAAGGAAACAAGCCATCTTGCAACAGCCAATTCTACTCAAATGACCGAACTCTGTCGCATTTTGCACGATTGGCTAAGATTTATAAAGCTTGGAAGTTCTACAGAATCCAGCTTGTACAGGAAGCCGCTGAAAAAGGTCTGCCTGTATGCCGCCACCTATTTCTGCATTACCCAGATGACGAACACGTCCATAACTTGACCTACCAGGAGTTTCTAATCGGCACCGAGATTTTAGTGGTGCCAGTTCTAGACAAAGGCAAGAATAATGTGAAGGCCTATTTTCCAAGAGGAGAAACTTCTTGTTGGGAACACATATGGAGTGGAAAACAATATTCAAAACAGGGTTCCGAAGCAACGGTAGACGCGCCGATTGGGTGTCCTGCTGTATTTGTTAAGACTGGCTCCATTGTTGGACAGACGTTTTTGAAAAATCTTAGAGATCTGAAGATTCTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Glyco_hydro_31

Protein Analysis

870

Amino Acids

99.57

Weight (kDa)

8.35

Isoelectric Point (pI)

48.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_31_2nd	PF01055	426 - 734	4.1e-36	Glycosyl hydrolases family 31 TIM-barrel domain
Glyco_hydro_31_3rd	PF21365	764 - 855	5.9e-17	Glycosyl hydrolase family 31 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007519)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g25360 FvH4_6g25360 FvH4_6g25360 FvH4_6g25360 FvH4_6g25360 FvH4_6g25360 FvH4_6g25360 FvH4_6g25360
malus_domestica	MD14G1090500.v1.1
prunus_persica	Prupe.7G033900_v2.0.a1 Prupe.7G033900_v2.0.a1 Prupe.7G033900_v2.0.a1 Prupe.7G033900_v2.0.a1
pyrus_communis	pycom14g08100
rosa_chinensis	RchiOBHm_Chr2g0125791
rosa_laevigata	RLG00000018880
rosa_multiflora	Rmu_sc0004466.1_g000001 Rmu_ssc0000055.1_g000006
rosa_roxburghii	Rroxscaffold_2G00117700
rosa_rugosa	Rorug02G0259300
rosa_samantha	Rh2AG317400 Rh2BG325500 Rh2CG307200 Rh2DG343400
rosa_wichuraiana	Rw2G025680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2238
AasI	GACNNNNNNGTC	1 cut(s)	2339
AccB1I	GGYRCC	2 cut(s)	2375, 2392
AccB7I	CCANNNNNTGG	1 cut(s)	662
AccI	GTMKAC	1 cut(s)	2514
AccII	CGCG	1 cut(s)	2519
AccIII	TCCGGA	2 cut(s)	1247, 2145
AciI	CCGC	7 cut(s)	118, 186, 727, 1272, 1898, 2280, 2305
AclWI	GGATC	3 cut(s)	204, 1387, 1757
AcoI	YGGCCR	2 cut(s)	247, 273
AcsI	RAATTY	1 cut(s)	821
AfaI	GTAC	6 cut(s)	1018, 1025, 1276, 1592, 2054, 2271
AfiI	CCNNNNNNNGG	7 cut(s)	504, 613, 662, 1059, 1649, 1922, 1923
AflIII	ACRYGT	1 cut(s)	2338
AjiI	CACGTC	1 cut(s)	2341
AjnI	CCWGG	3 cut(s)	765, 933, 2358
AjuI	GAANNNNNNNTTGG	2 cut(s)	930, 962
Alw21I	GWGCWC	4 cut(s)	644, 1188, 1308, 1459
Alw26I	GTCTC	4 cut(s)	312, 1068, 1361, 1938
AlwI	GGATC	3 cut(s)	204, 1387, 1757
Aor13HI	TCCGGA	2 cut(s)	1247, 2145
AoxI	GGCC	8 cut(s)	247, 273, 542, 703, 749, 1357, 1996, 2427
ApeKI	GCWGC	4 cut(s)	266, 724, 977, 1342
ApoI	RAATTY	1 cut(s)	821
Asp700I	GAANNNNTTC	1 cut(s)	1728
AspLEI	GCGC	2 cut(s)	720, 2521
AspS9I	GGNCC	6 cut(s)	171, 234, 542, 931, 1170, 1357
AsuC2I	CCSGG	2 cut(s)	246, 608
AsuHPI	GGTGA	9 cut(s)	125, 194, 338, 382, 622, 968, 1167, 1733, 1769
AsuII	TTCGAA	1 cut(s)	1847
AvaII	GGWCC	4 cut(s)	171, 234, 931, 1170
BaeI	ACNNNNGTAYC	2 cut(s)	670, 703
BanI	GGYRCC	2 cut(s)	2375, 2392
BanII	GRGCYC	4 cut(s)	229, 305, 1188, 1308
BbsI	GAAGAC	3 cut(s)	1222, 1721, 1869
Bbv12I	GWGCWC	4 cut(s)	644, 1188, 1308, 1459
BbvI	GCAGC	4 cut(s)	278, 711, 989, 1329
BcgI	CGANNNNNNTGC	2 cut(s)	1655, 1689
BciT130I	CCWGG	3 cut(s)	767, 935, 2360
BclI	TGATCA	4 cut(s)	372, 592, 958, 987
BcnI	CCSGG	2 cut(s)	246, 608
BcoDI	GTCTC	4 cut(s)	312, 1068, 1361, 1938
BfaI	CTAG	3 cut(s)	701, 1496, 2402
BfmI	CTRYAG	4 cut(s)	264, 994, 1343, 2254
BfoI	RGCGCY	1 cut(s)	721
BglII	AGATCT	2 cut(s)	1447, 2593
BisI	GCNGC	7 cut(s)	187, 267, 725, 978, 1343, 2280, 2305
BlpI	GCTNAGC	1 cut(s)	1458
BlsI	GCNGC	7 cut(s)	188, 268, 726, 979, 1344, 2281, 2306
Bme1390I	CCNGG	5 cut(s)	246, 608, 767, 935, 2360
Bme18I	GGWCC	4 cut(s)	171, 234, 931, 1170
BmgBI	CACGTC	1 cut(s)	2341
BmgT120I	GGNCC	6 cut(s)	171, 234, 542, 931, 1170, 1357
BmiI	GGNNCC	9 cut(s)	304, 543, 921, 1171, 1599, 2377, 2394, 2500, 2557
BmrFI	CCNGG	5 cut(s)	246, 608, 767, 935, 2360
BmrI	ACTGGG	1 cut(s)	437
BmsI	GCATC	4 cut(s)	1309, 1729, 1864, 1917
BmuI	ACTGGG	1 cut(s)	437
BpiI	GAAGAC	3 cut(s)	1222, 1721, 1869
BplI	GAGNNNNNCTC	2 cut(s)	502, 534
Bpu1102I	GCTNAGC	1 cut(s)	1458
Bpu14I	TTCGAA	1 cut(s)	1847
BpuEI	CTTGAG	3 cut(s)	785, 792, 1292
BpuMI	CCSGG	2 cut(s)	246, 608
BsaI	GGTCTC	2 cut(s)	1068, 1938
BsaJI	CCNNGG	2 cut(s)	85, 244
BsaWI	WCCGGW	2 cut(s)	1247, 2145
BsaXI	ACNNNNNCTCC	4 cut(s)	1012, 1042, 1242, 1272
Bsc4I	CCNNNNNNNGG	7 cut(s)	504, 613, 662, 1059, 1649, 1922, 1923
Bse118I	RCCGGY	2 cut(s)	705, 720
Bse1I	ACTGG	8 cut(s)	438, 443, 484, 1241, 1443, 1654, 2396, 2557
Bse3DI	GCAATG	1 cut(s)	1686
BseAI	TCCGGA	2 cut(s)	1247, 2145
BseBI	CCWGG	3 cut(s)	767, 935, 2360
BseDI	CCNNGG	2 cut(s)	85, 244
BseLI	CCNNNNNNNGG	7 cut(s)	504, 613, 662, 1059, 1649, 1922, 1923
BseMI	GCAATG	1 cut(s)	1686
BseMII	CTCAG	6 cut(s)	273, 533, 1158, 1173, 1472, 2017
BseNI	ACTGG	8 cut(s)	438, 443, 484, 1241, 1443, 1654, 2396, 2557
BseRI	GAGGAG	4 cut(s)	71, 709, 1868, 2457
BseXI	GCAGC	4 cut(s)	278, 711, 989, 1329
BseYI	CCCAGC	1 cut(s)	483
Bsh1236I	CGCG	1 cut(s)	2519
Bsh1285I	CGRYCG	2 cut(s)	427, 468
BshFI	GGCC	8 cut(s)	249, 275, 544, 705, 751, 1359, 1998, 2429
BshNI	GGYRCC	2 cut(s)	2375, 2392
BsiEI	CGRYCG	2 cut(s)	427, 468
BsiHKAI	GWGCWC	4 cut(s)	644, 1188, 1308, 1459
BsiSI	CCGG	6 cut(s)	246, 607, 706, 721, 1248, 2146
BsiWI	CGTACG	1 cut(s)	1023
BslFI	GGGAC	3 cut(s)	633, 1183, 1899
BslI	CCNNNNNNNGG	7 cut(s)	504, 613, 662, 1059, 1649, 1922, 1923
BsmAI	GTCTC	4 cut(s)	312, 1068, 1361, 1938
BsmBI	CGTCTC	1 cut(s)	1361
BsmFI	GGGAC	3 cut(s)	633, 1183, 1899
BsnI	GGCC	8 cut(s)	249, 275, 544, 705, 751, 1359, 1998, 2429
Bso31I	GGTCTC	2 cut(s)	1068, 1938
Bsp119I	TTCGAA	1 cut(s)	1847
Bsp1286I	GDGCHC	6 cut(s)	229, 305, 644, 1188, 1308, 1459
Bsp13I	TCCGGA	2 cut(s)	1247, 2145
Bsp1407I	TGTACA	1 cut(s)	2269
Bsp1720I	GCTNAGC	1 cut(s)	1458
BspACI	CCGC	7 cut(s)	118, 186, 727, 1272, 1898, 2280, 2305
BspANI	GGCC	8 cut(s)	249, 275, 544, 705, 751, 1359, 1998, 2429
BspCNI	CTCAG	6 cut(s)	274, 532, 1157, 1174, 1471, 2016
BspEI	TCCGGA	2 cut(s)	1247, 2145
BspFNI	CGCG	1 cut(s)	2519
BspHI	TCATGA	1 cut(s)	1507
BspLI	GGNNCC	9 cut(s)	304, 543, 921, 1171, 1599, 2377, 2394, 2500, 2557
BspMAI	CTGCAG	2 cut(s)	268, 1347
BspPI	GGATC	3 cut(s)	204, 1387, 1757
BspT104I	TTCGAA	1 cut(s)	1847
BspT107I	GGYRCC	2 cut(s)	2375, 2392
BspTNI	GGTCTC	2 cut(s)	1068, 1938
BsrDI	GCAATG	1 cut(s)	1686
BsrFI	RCCGGY	2 cut(s)	705, 720
BsrGI	TGTACA	1 cut(s)	2269
BsrI	ACTGG	8 cut(s)	438, 443, 484, 1241, 1443, 1654, 2396, 2557
BssAI	RCCGGY	2 cut(s)	705, 720
BssECI	CCNNGG	2 cut(s)	85, 244
BssT1I	CCWWGG	1 cut(s)	85
Bst2UI	CCWGG	3 cut(s)	767, 935, 2360
Bst4CI	ACNGT	5 cut(s)	590, 2039, 2057, 2140, 2512
Bst6I	CTCTTC	3 cut(s)	780, 908, 2091
BstAUI	TGTACA	1 cut(s)	2269
BstBI	TTCGAA	1 cut(s)	1847
BstC8I	GCNNGC	3 cut(s)	722, 1265, 1288
BstDEI	CTNAG	9 cut(s)	282, 519, 1144, 1182, 1451, 1458, 2003, 2229, 2588
BstFNI	CGCG	1 cut(s)	2519
BstH2I	RGCGCY	1 cut(s)	721
BstHHI	GCGC	2 cut(s)	720, 2521
BstMAI	GTCTC	4 cut(s)	312, 1068, 1361, 1938
BstMCI	CGRYCG	2 cut(s)	427, 468
BstMWI	GCNNNNNNNGC	4 cut(s)	272, 724, 1269, 2225
BstNI	CCWGG	3 cut(s)	767, 935, 2360
BstNSI	RCATGY	1 cut(s)	1267
BstSCI	CCNGG	5 cut(s)	244, 606, 765, 933, 2358
BstSFI	CTRYAG	4 cut(s)	264, 994, 1343, 2254
BstUI	CGCG	1 cut(s)	2519
BstV1I	GCAGC	4 cut(s)	278, 711, 989, 1329
BstV2I	GAAGAC	3 cut(s)	1222, 1721, 1869
BstX2I	RGATCY	3 cut(s)	1447, 1762, 2593
BstXI	CCANNNNNNTGG	1 cut(s)	1797
BstYI	RGATCY	3 cut(s)	1447, 1762, 2593
BsuRI	GGCC	8 cut(s)	249, 275, 544, 705, 751, 1359, 1998, 2429
BtgZI	GCGATG	1 cut(s)	2125
BtrI	CACGTC	1 cut(s)	2341
BtsIMutI	CAGTG	3 cut(s)	595, 1200, 2044
Cac8I	GCNNGC	3 cut(s)	722, 1265, 1288
CciI	TCATGA	1 cut(s)	1507
CfoI	GCGC	2 cut(s)	720, 2521
Cfr10I	RCCGGY	2 cut(s)	705, 720
Cfr13I	GGNCC	6 cut(s)	171, 234, 542, 931, 1170, 1357
CseI	GACGC	2 cut(s)	2158, 2525
Csp6I	GTAC	6 cut(s)	1017, 1024, 1275, 1591, 2053, 2270
CspCI	CAANNNNNGTGG	4 cut(s)	149, 184, 1373, 1408
CviAII	CATG	7 cut(s)	1136, 1264, 1491, 1508, 1580, 1594, 2153
CviQI	GTAC	6 cut(s)	1017, 1024, 1275, 1591, 2053, 2270
DdeI	CTNAG	9 cut(s)	282, 519, 1144, 1182, 1451, 1458, 2003, 2229, 2588
DrdI	GACNNNNNNGTC	1 cut(s)	2339
DseDI	GACNNNNNNGTC	1 cut(s)	2339
EaeI	YGGCCR	2 cut(s)	247, 273
Eam1104I	CTCTTC	3 cut(s)	780, 908, 2091
EarI	CTCTTC	3 cut(s)	780, 908, 2091
EciI	GGCGGA	1 cut(s)	107
Ecl136II	GAGCTC	2 cut(s)	1186, 1306
Eco130I	CCWWGG	1 cut(s)	85
Eco147I	AGGCCT	1 cut(s)	2429
Eco24I	GRGCYC	4 cut(s)	229, 305, 1188, 1308
Eco31I	GGTCTC	2 cut(s)	1068, 1938
Eco47I	GGWCC	4 cut(s)	171, 234, 931, 1170
Eco53kI	GAGCTC	2 cut(s)	1186, 1306
EcoICRI	GAGCTC	2 cut(s)	1186, 1306
EcoO109I	RGGNCCY	2 cut(s)	542, 1170
EcoRI	GAATTC	1 cut(s)	821
EcoRII	CCWGG	3 cut(s)	765, 933, 2358
EcoT14I	CCWWGG	1 cut(s)	85
EcoT22I	ATGCAT	2 cut(s)	1879, 2029
EcoT38I	GRGCYC	4 cut(s)	229, 305, 1188, 1308
ErhI	CCWWGG	1 cut(s)	85
Esp3I	CGTCTC	1 cut(s)	1361
FaeI	CATG	7 cut(s)	1139, 1267, 1494, 1511, 1583, 1597, 2156
FalI	AAGNNNNNCTT	2 cut(s)	2088, 2120
FaqI	GGGAC	3 cut(s)	633, 1183, 1899
FatI	CATG	7 cut(s)	1135, 1263, 1490, 1507, 1579, 1593, 2152
FauI	CCCGC	1 cut(s)	1891
FauNDI	CATATG	2 cut(s)	897, 2468
FbaI	TGATCA	4 cut(s)	372, 592, 958, 987
FblI	GTMKAC	1 cut(s)	2514
Fnu4HI	GCNGC	7 cut(s)	187, 267, 725, 978, 1343, 2280, 2305
FriOI	GRGCYC	4 cut(s)	229, 305, 1188, 1308
Fsp4HI	GCNGC	7 cut(s)	187, 267, 725, 978, 1343, 2280, 2305
FspBI	CTAG	3 cut(s)	701, 1496, 2402
GlaI	GCGC	2 cut(s)	719, 2520
GluI	GCNGC	7 cut(s)	187, 267, 725, 978, 1343, 2280, 2305
GsaI	CCCAGC	1 cut(s)	487
HaeII	RGCGCY	1 cut(s)	721
HaeIII	GGCC	8 cut(s)	249, 275, 544, 705, 751, 1359, 1998, 2429
HapII	CCGG	6 cut(s)	246, 607, 706, 721, 1248, 2146
HgaI	GACGC	2 cut(s)	2158, 2525
HhaI	GCGC	2 cut(s)	720, 2521
Hin1II	CATG	7 cut(s)	1139, 1267, 1494, 1511, 1583, 1597, 2156
Hin6I	GCGC	2 cut(s)	718, 2519
HinP1I	GCGC	2 cut(s)	718, 2519
HindIII	AAGCTT	3 cut(s)	326, 648, 2241
HpaII	CCGG	6 cut(s)	246, 607, 706, 721, 1248, 2146
HphI	GGTGA	9 cut(s)	125, 194, 338, 382, 622, 968, 1167, 1733, 1769
Hpy166II	GTNNAC	4 cut(s)	237, 2012, 2065, 2515
Hpy8I	GTNNAC	4 cut(s)	237, 2012, 2065, 2515
HpyCH4III	ACNGT	5 cut(s)	590, 2039, 2057, 2140, 2512
HpyCH4IV	ACGT	5 cut(s)	1022, 1370, 1589, 2340, 2573
HpyF10VI	GCNNNNNNNGC	4 cut(s)	272, 724, 1269, 2225
HpyF3I	CTNAG	9 cut(s)	282, 519, 1144, 1182, 1451, 1458, 2003, 2229, 2588
HpySE526I	ACGT	5 cut(s)	1022, 1370, 1589, 2340, 2573
Hsp92II	CATG	7 cut(s)	1139, 1267, 1494, 1511, 1583, 1597, 2156
HspAI	GCGC	2 cut(s)	718, 2519
Kpn2I	TCCGGA	2 cut(s)	1247, 2145
KroI	GCCGGC	1 cut(s)	720
KroNI	GCCGGC	1 cut(s)	722
Ksp22I	TGATCA	4 cut(s)	372, 592, 958, 987
LmnI	GCTCC	8 cut(s)	308, 883, 1033, 1250, 1327, 1504, 2119, 2561
Lsp1109I	GCAGC	4 cut(s)	278, 711, 989, 1329
LweI	GCATC	4 cut(s)	1309, 1729, 1864, 1917
MaeI	CTAG	3 cut(s)	701, 1496, 2402
MaeII	ACGT	5 cut(s)	1022, 1370, 1589, 2340, 2573
MaeIII	GTNAC	5 cut(s)	445, 475, 851, 1901, 2039
MflI	RGATCY	3 cut(s)	1447, 1762, 2593
MhlI	GDGCHC	6 cut(s)	229, 305, 644, 1188, 1308, 1459
MluCI	AATT	8 cut(s)	103, 452, 458, 635, 821, 1396, 1806, 2183
MlyI	GAGTC	1 cut(s)	1704
MmeI	TCCRAC	5 cut(s)	771, 1357, 1536, 1608, 2545
Mph1103I	ATGCAT	2 cut(s)	1879, 2029
MroI	TCCGGA	2 cut(s)	1247, 2145
MroNI	GCCGGC	1 cut(s)	720
MroXI	GAANNNNTTC	1 cut(s)	1728
MseI	TTAA	6 cut(s)	906, 1443, 1809, 2031, 2079, 2547
MslI	CAYNNNNRTG	2 cut(s)	1387, 1565
MspA1I	CMGCKG	1 cut(s)	2282
MspI	CCGG	6 cut(s)	246, 607, 706, 721, 1248, 2146
MspR9I	CCNGG	5 cut(s)	246, 608, 767, 935, 2360
MvaI	CCWGG	3 cut(s)	767, 935, 2360
MvnI	CGCG	1 cut(s)	2519
MwoI	GCNNNNNNNGC	4 cut(s)	272, 724, 1269, 2225
NaeI	GCCGGC	1 cut(s)	722
NciI	CCSGG	2 cut(s)	246, 608
NdeI	CATATG	2 cut(s)	897, 2468
NgoMIV	GCCGGC	1 cut(s)	720
NlaIII	CATG	7 cut(s)	1139, 1267, 1494, 1511, 1583, 1597, 2156
NlaIV	GGNNCC	9 cut(s)	304, 543, 921, 1171, 1599, 2377, 2394, 2500, 2557
NmeAIII	GCCGAG	1 cut(s)	126
NmuCI	GTSAC	2 cut(s)	851, 2039
NsiI	ATGCAT	2 cut(s)	1879, 2029
NspI	RCATGY	1 cut(s)	1267
NspV	TTCGAA	1 cut(s)	1847
PaeI	GCATGC	1 cut(s)	1267
PagI	TCATGA	1 cut(s)	1507
PceI	AGGCCT	1 cut(s)	2429
PdiI	GCCGGC	1 cut(s)	722
PdmI	GAANNNNTTC	1 cut(s)	1728
Pfl23II	CGTACG	1 cut(s)	1023
PflMI	CCANNNNNTGG	1 cut(s)	662
PfoI	TCCNGGA	1 cut(s)	933
PkrI	GCNGC	7 cut(s)	188, 268, 726, 979, 1344, 2281, 2306
Ple19I	CGATCG	2 cut(s)	427, 468
PleI	GAGTC	1 cut(s)	1703
PpsI	GAGTC	1 cut(s)	1703
PpuMI	RGGWCCY	1 cut(s)	1170
PsiI	TTATAA	1 cut(s)	2238
Psp124BI	GAGCTC	2 cut(s)	1188, 1308
Psp5II	RGGWCCY	1 cut(s)	1170
Psp6I	CCWGG	3 cut(s)	765, 933, 2358
PspFI	CCCAGC	1 cut(s)	483
PspGI	CCWGG	3 cut(s)	765, 933, 2358
PspLI	CGTACG	1 cut(s)	1023
PspN4I	GGNNCC	9 cut(s)	304, 543, 921, 1171, 1599, 2377, 2394, 2500, 2557
PspPI	GGNCC	6 cut(s)	171, 234, 542, 931, 1170, 1357
PspPPI	RGGWCCY	1 cut(s)	1170
PsrI	GAACNNNNNNTAC	2 cut(s)	652, 684
PstI	CTGCAG	2 cut(s)	268, 1347
PsuI	RGATCY	3 cut(s)	1447, 1762, 2593
PvuI	CGATCG	2 cut(s)	427, 468
RsaI	GTAC	6 cut(s)	1018, 1025, 1276, 1592, 2054, 2271
RsaNI	GTAC	6 cut(s)	1017, 1024, 1275, 1591, 2053, 2270
RseI	CAYNNNNRTG	2 cut(s)	1387, 1565
SacI	GAGCTC	2 cut(s)	1188, 1308
SaqAI	TTAA	6 cut(s)	906, 1443, 1809, 2031, 2079, 2547
SatI	GCNGC	7 cut(s)	187, 267, 725, 978, 1343, 2280, 2305
Sau96I	GGNCC	6 cut(s)	171, 234, 542, 931, 1170, 1357
SchI	GAGTC	1 cut(s)	1704
ScrFI	CCNGG	5 cut(s)	246, 608, 767, 935, 2360
SduI	GDGCHC	6 cut(s)	229, 305, 644, 1188, 1308, 1459
SfaNI	GCATC	4 cut(s)	1309, 1729, 1864, 1917
SfcI	CTRYAG	4 cut(s)	264, 994, 1343, 2254
SfuI	TTCGAA	1 cut(s)	1847
SinI	GGWCC	4 cut(s)	171, 234, 931, 1170
SmiMI	CAYNNNNRTG	2 cut(s)	1387, 1565
SmlI	CTYRAG	3 cut(s)	800, 807, 1307
SmoI	CTYRAG	3 cut(s)	800, 807, 1307
SphI	GCATGC	1 cut(s)	1267
Sse9I	AATT	8 cut(s)	103, 452, 458, 635, 821, 1396, 1806, 2183
SseBI	AGGCCT	1 cut(s)	2429
SsiI	CCGC	7 cut(s)	118, 186, 727, 1272, 1898, 2280, 2305
SspI	AATATT	2 cut(s)	1088, 2486
SspMI	CTAG	3 cut(s)	701, 1496, 2402
SstI	GAGCTC	2 cut(s)	1188, 1308
StuI	AGGCCT	1 cut(s)	2429
StyD4I	CCNGG	5 cut(s)	244, 606, 765, 933, 2358
StyI	CCWWGG	1 cut(s)	85
TaaI	ACNGT	5 cut(s)	590, 2039, 2057, 2140, 2512
TaiI	ACGT	5 cut(s)	1025, 1373, 1592, 2343, 2576
TaqI	TCGA	6 cut(s)	423, 848, 1074, 1675, 1847, 2086
TaqII	GACCGA	1 cut(s)	2214
TasI	AATT	8 cut(s)	103, 452, 458, 635, 821, 1396, 1806, 2183
TatI	WGTACW	2 cut(s)	1016, 2269
TauI	GCSGC	3 cut(s)	189, 2282, 2307
Tru1I	TTAA	6 cut(s)	906, 1443, 1809, 2031, 2079, 2547
Tru9I	TTAA	6 cut(s)	906, 1443, 1809, 2031, 2079, 2547
TscAI	CASTG	3 cut(s)	595, 1207, 2044
TseFI	GTSAC	2 cut(s)	851, 2039
TseI	GCWGC	4 cut(s)	266, 724, 977, 1342
Tsp45I	GTSAC	2 cut(s)	851, 2039
TspDTI	ATGAA	6 cut(s)	320, 1098, 1842, 1875, 2065, 2169
TspGWI	ACGGA	3 cut(s)	1081, 1491, 1832
TspRI	CASTG	3 cut(s)	595, 1207, 2044
Van91I	CCANNNNNTGG	1 cut(s)	662
VpaK11BI	GGWCC	4 cut(s)	171, 234, 931, 1170
XapI	RAATTY	1 cut(s)	821
XbaI	TCTAGA	1 cut(s)	2401
XceI	RCATGY	1 cut(s)	1267
XmiI	GTMKAC	1 cut(s)	2514
XmnI	GAANNNNTTC	1 cut(s)	1728
XspI	CTAG	3 cut(s)	701, 1496, 2402
Zsp2I	ATGCAT	2 cut(s)	1879, 2029

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.